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OPTIMIZATION OF KINETIN CONCENTRATIONS AND MEDIUM COMPOSITIONS FOR CITRUS SHOOT MULTIPLICATION FROM COTILEDONARY NODE Kristianto Nugroho; Mia Kosmiatin; Tri Joko Santoso; Dewi Sukma; Agus Purwito; Ali Husni; Chaireni Martasari
BIOTROPIA Vol. 31 No. 1 (2024): BIOTROPIA Vol. 31 No. 1 April 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.1.2136

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Plant regeneration post-genetic transformation play an important an role in genome editing activities that should be optimized via several factors such as the composition of the medium and the concentration of plant growth regulators. This study aimed to optimize the kinetin concentrations and medium compositions for shoot multiplication originating from cotyledon node explants of several local citrus cultivars. The cotyledonary nodes from three citrus cultivars (Batu 55, Siam Madu, and Proksi-1 Agrihorti) were incubated in MS medium with Morel and Wetmore vitamins (VMW) supplemented with several kinetin concentrations (0; 0.2; 0.4; 0.6; 0.8; and 1 mg/L). The best kinetin concentrations for number of shoots variable were then combined with MT medium. The results showed that kinetin concentration at 0.8 mg/L gave the best number of shoot in Batu 55 cultivar as well as 1 mg/L concentration in Siam Madu and Proksi-1 Agrihorti cultivars. The combination 1 mg/L kinetin with Murashige and Tucker (MT) medium showed the best number of shoots, percentage of shoot formation, number of leaves, number of nodes, and shoot length in this study. This medium composition could be further used for shoot multiplication in genetic transformation in those three citrus cultivars, including genome editing activities in development of new improved citrus varieties.
METABOLITE PROFILING OF MORINGA USING PY-GCMS AND TOLERANCE EVALUATION TO ALUMINUM ON IN VITRO CULTURE Rudiyanto Rudiyanto; Agus Purwito Purwito; Darda Efendi; Andri Martin
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2354

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Article Highlight: Four accessions of Moringa oleifera from different regions in Indonesia were evaluated to identify with tolerance to aluminium (Al) stress Eight metabolites show a very high correlation with acetic acid (one of the metabolites associated with AlCl3 stress) including cyclopentene, 2-allyphenol, 4-ethynyl-6-8-dioxane, vinyl ether, ethanone 1-oxiranyl, 2-methylpyridine, 2-butanone, and ethanesulfonic acid. This research makes a significant contribution to understanding aluminium tolerance in oleifera by identifying tolerant accessions, clustering relevant traits, and highlighting key metabolites to in vitro culture. ABSTRACT Moringa oleifera Linn. has received substantial scientific interest due to its numerous bioactive compounds and its function as a nutritional resource. The absorption of aluminum by plants hinders several metabolic and physiological processes, leading to inhibited plant development and decreased agricultural output. Some accessions from different regions in Indonesia were evaluated to identify those with tolerance to aluminum (Al) stress. Al-tolerant selection was carried out in vitro through the selection method for Al stress by adding 0, 50, 100, 250, and 500 mg/L of AlCl3 to the media. Furthermore, identifying the metabolite profile of four M. oleifera accessions from four distinct regions in Indonesia: Blora, Bogor, Enrekang, and Bima, has been done using Py-GCMS. Specific metabolites associated with tolerance to Al stress and organic acids need to be identified. The highest survival rate was observed in the Bogor and Blora accessions when exposed to AlCl₃ at concentrations ranging from 0 to 250 mg/L, demonstrating greater tolerance to AlCl₃ than other accessions based on various variable, such as shoot height, number of shoots, number of petioles on a medium containing 100 to 250 mg/L of AlCl₃. The mean value was not statistically different from the control. Acetic acid was identified as one of the metabolites associated with AlCl₃ stress. A total of 21 metabolites were specifically correlated with acetic acid in a positive manner, among which 8 metabolites including cyclopentene, 2-allyphenol, 4-ethynyl-6-8-dioxane, vinyl ether, ethanone 1-oxiranyl, 2-methylpyridine, 2-butanone, and ethanesulfonic acid exhibited a very high correlation.
Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches JANE KATHRYNE JOLANDA LAISINA; AWANG MAHARIJAYA; SOBIR SOBIR; AGUS PURWITO
Biodiversitas Journal of Biological Diversity Vol. 22 No. 2 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220204

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Abstract. Laisina JKJ, Maharijaya A, Sobir, Purwito A. 2021. Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches. Biodiversitas 22: 537-545. Prediction of drought adaptive potatoes requires selection indicators. This study aimed to determine in vitro selection indicators for obtaining drought adaptive potato genotypes in vitro and in vivo. The in vitro study was performed using single-node explants from seven genotypes cultured for six weeks on MS medium added with 0.2 M sorbitol to achieve the research aims. The cultures were partially observed and followed by watering the in vitro tubing medium. For the in vivo study, drought stress was applied for 21 days when the plants reached 25 days after planting (DAP) and for 14 days when the plants reached 50 DAP, then plants were irrigated again until harvesting. The results showed that plant height and root length can be used as selection indicators because they had a relative decrease below 50%, a close relationship and had the same variance with the in vitro number of tubers, in vitro tuber fresh weight and in vivo tuber fresh weight in drought stress at 25 DAP. These characters were determinant characters for the clustering pattern in the heat map cluster analysis. Based on these characters, SSI analysis was also performed and it was found that the PKHT4 was more adaptive than the PKHT6 in vitro, but the two genotypes were classified in the medium adaptive in vivo. In conclusion, plant height and root length were determined as the in vitro selection indicators in predicting drought adaptive genotypes in vivo at the plant age of 25 DAP.
Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms Halida Adistya Putri; Agus Purwito; Sudarsono Sudarsono; Dewi Sukma
Biodiversitas Journal of Biological Diversity Vol. 22 No. 3 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220301

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Abstract. Putri HA, Purwito A, Sudarsono, Sukma D. 2021. Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms. Biodiversitas 22: 1077-1090. Phalaenopsis amabilis (L.) Blume is a prominent donor for the white petal and sepal trait in Phalaenopsis breeding. However, it has an undesirable character, such as susceptible to soft-rot disease. Therefore, developing soft-rot resistance mutants through gamma irradiation could be explored. This study aimed to evaluate the variability of plantlets regenerated from irradiated and non-irradiated protocorms using morphology, stomatal size and molecular markers and to test responses of the plantlets against soft-rot disease. The plantlets were regenerated from irradiated (5, 10, 15 or 20 Gy) and non-irradiated protocorms. The results showed that P. amabilis plantlet variants were successfully identified based on their leaf morphology and stomatal size variations. A few plantlets have low stomatal densities, large stomatal size, and high chloroplast numbers, which indicated they were polyploids. Leaf disc assay for soft-rot disease response grouped most of the plantlets into very susceptible or susceptible. Moreover, four soft-rot resistant plantlets regenerated from irradiated and non-irradiated protocorms were successfully identified. The resistant plantlets were identified after three consecutive periods of inoculations with pathogens causing soft-rot disease. The evaluation also confirmed nucleotide variation in the Pto gene isolated from different levels of plantlet variant resistance responses.
Genetic diversity analysis of Puan Kalianda kopyor coconuts (Cocos nucifera) from South Lampung, Indonesia based on SSR markers Megayani Sri Rahayu; Asep Setiawan; Ismail Maskromo; Agus Purwito; Sudarsono Sudarsono
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230126

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Abstract. Rahayu MS, Setiawan A, Maskromo I, Purwito A, Sudarsono. 2021. Genetic diversity analysis of Puan Kalianda kopyor coconuts (Cocos nucifera) from South Lampung, Indonesia based on SSR markers. Biodiversitas 23: 205-211. Puan Kalianda kopyor coconut (Cocos nucifera L.) is a newly released tall kopyor coconut from Kalianda, South Lampung, Indonesia. The kopyor coconut is an exotic, highly economic value coconut mutant with an abnormal endosperm. This study aimed to analyze the genetic diversity of Puan Kalianda kopyor coconut from South Lampung, Indonesia using SSR markers. As many as 91 Puan Kalianda kopyor coconut accessions were genotyped using 10 SSR marker loci, and the generated data were used to evaluate their genetic diversity and population structure. The results showed a high degree of SSR marker polymorphism (PIC value = 67%), indicating the SSR marker loci are informative for revealing the genetic diversity within the evaluated Puan Kalianda kopyor coconut population. The Puan Kalianda coconut population showed a 70% expected heterozygosity (He) and 60% observed heterozygosity. The phylogenetic analysis formed two main clusters, and each cluster consisted of three sub-clusters. The Genetic structure analysis showed that the population most probably derived from two ancestral origins (K = 2) and can further be clustered into six sub-clusters (K = 6). Therefore, since genetic diversity within the population is relatively high, the Puan Kalianda tall kopyor coconut population can be considered an essential genetic resource for future kopyor coconut development.
Evaluation of agro-morphological and molecular characters of 22 rice landraces of East Timor LUIS MANUEL BRANCO; ENDAH RETNO PALUPI; SATRIYAS ILYAS; BAMBANG SAPTA PURWOKO; AGUS PURWITO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 5 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240505

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Abstract. Branco LM, Palupi ER, Ilyas S, Purwoko BS, Purwito A. 2023. Evaluation of agro-morphological and molecular characters of 22 rice landraces of East Timor. Biodiversitas 24: 2536-2546. Landraces play an important role in the local food security and sustainable development of agriculture as a source of genes that control important traits adaptive to environmental conditions. The study aimed to characterize the agro-morphological and molecular characteristics of 22 rice landraces of East Timor (Timor Leste). The research was conducted in April-August 2018 in Caibada-Baucau-East Timor. Soil analysis was conducted at the Soil Laboratory of the Agronomy and Horticulture Department, IPB University. Materials used were 22 rice landraces seeds and one check variety. The seeds were germinated in seedbed seedlings with a basin (30 cm x 25 cm). The seedlings were planted in the field 16 days after sowing in a plot of 1.5 m x 7.0 m and a spacing of 25 cm x 25 cm, with an isolation distance between plots of ±3 m. Data collection was based on nine growth phases of rice. The cluster analysis based on agro-morphological characters resulted in 6 groups with a similarity coefficient of 0.17 or 17%, showing a high level of diversity. The lowest yield was Ale Kukefuhu (290 g/m2), and the highest was Hare Nona Portu (979 g/m2). Using 10 RAPD markers, the 22 rice landraces samples produced 77 DNA fragment bands with sizes between 300 bp-1475 bp, averaging 3.35 alleles per landrace; polymorphic rates of 8.1. OPA8, OPH1, and OPH7 markers were the highest (100.00%), and OPF5 was the lowest (66.67%) polymorphic percentage. Based on the molecular marker, the coefficient of similarity of the landraces ranged from 0.12 to 0.51, and they were grouped into five clusters.
Unraveling metabolite profile variations among resistance and susceptible shallot genotypes related to anthracnose (Colletotrichum gloeosporioides) RIZKI ABI AMRULLAH; AWANG MAHARIJAYA; AGUS PURWITO; SURYO WIYONO
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240956

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Abstract. Amrullah RA, Maharijaya A, Purwito A, Wiyono S. 2023. Unraveling metabolite profile variations among resistance and susceptible shallot genotypes related to anthracnose (Colletotrichum gloeosporioides). Biodiversitas 24: 5113-5122.This study used a metabolomics approach to reveal the shallot metabolite profile under anthracnose disease conditions through GC-MS analysis. The aim of this study was to obtain candidate metabolite markers that differentiate shallot genotypes resistant to anthracnose. The results of non-targeted metabolite analysis with GC-MS revealed a shallot metabolite profiles of 54 compounds. The principal component analysis (PCA) model was conducted for reliable and accurate discrimination between uninoculated and inoculated genotypes. Four metabolites were revealed as putative biomarkers of Colletotrichum gloeosporioides Penz. infection, namely linoleic ethyl ester, squalene, octadecanoic acid, and nonacosane. At least some putative biomarkers were applicable for early resistance genotype detection in shallot. These metabolites may help characterize pathogen infection and plant defense responses. This study confirmed metabolomics as a tool to develop a strategy to clarify the mechanism of plant-pathogen interaction. Furthermore, the data presented may be helpful to developing a new method for detecting shallot, that may be resistant to anthracnose caused by C. gloeosporioides.
Characteristics and quality of cashew (Anacardium occidentale) seeds across various agroecological conditions MUHAMMAD H. ANUGRAH; MELATI MELATI; AGUS PURWITO; ENDAH RETNO PALUPI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 11 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251140

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Abstract. Anugrah MH, Melati, Purwito A, Palupi ER. 2024. Characteristics and quality of cashew (Anacardium occidentale) seeds across various agroecological conditions. Biodiversitas 25: 4406-4415. Cashew (Anacardium occidentale L.; Anacardiaceae) is a major plantation commodity developed mainly on marginal lands in Eastern Indonesia. This commodity is cultivated in various agroecological conditions, including wet, moderate, and dry climates. This research aims to provide information on the characteristics and quality of cashew seeds from different agroecological conditions and to explore the relationship between these conditions and seed storability. The research was conducted from November 2022 to December 2023. Seeds from two varieties were collected from seed orchards in various agroecological conditions. Seeds of Balakrisnan 02 variety were collected from a wet climate region (Bantul, Yogyakarta), and a moderate climate region (Sumbawa, West Nusa Tenggara). Seeds of Flotim variety were collected from a moderate climate region (Bangkalan, East Java), and a dry climate region (East Flores, East Nusa Tenggara). The seeds were stored at 16°C and 50% RH for six months. Physical, and physiological quality were observed during the storage. The result showed that drier agroecological conditions produce better physical quality seeds, such as larger seeds with thicker nut shells (fruit skin), and higher peroxidase activity and proline content. Planting the Balakrisnan 02 variety in moderate climate regions produces seeds with higher viability and vigor and longer storage capacity than those in wet climate regions. Meanwhile, Flotim, the drought-tolerant variety, when planted in the dry climate region produces seeds with higher viability but lower vigor and shorter storage capacity than those in moderate climate regions. Despite the differences in seed quality, planting the same variety in different agroecological conditions is still recommended to ensure seed availability due to different harvest times.
Analisis Ketahanan Klonal Anggrek Phalaenopsis Terhadap Bakteri Dickeya Dadantii Mira Humaira; Agus Purwito; Sudarsono Sudarsono; Dewi Sukma
JOURNAL OF SCIENCE AND SOCIAL RESEARCH Vol. 8 No. 4 (2025): November 2025
Publisher : Smart Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54314/jssr.v8i4.4857

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Abstract: Phalaenopsis orchids are one of the ornamental plants that are very popular with the public because they have attractive flower colors and shapes. One of the obstacles in the success of orchid cultivation is the attack of soft rot disease caused by Dickeya dadantii. Therefore, efforts are needed to find Phalaenopsis clones that are resistant to soft rot disease. This study aims to identify the resistance response of the hybrid orchid genotype Phal. ‘Salu Spot’ × Phal. bellina no. 1102-44. The plant material used was the hybrid orchid Phal. ‘Salu Spot’ × Phal. bellina no. 1102-44 inoculated with Dickeya dadantii bacteria. Identification of the resistance response was carried out through the infection method using the leaf disk technique. The results showed that the clone Phal. ‘Salu Spot’ × Phal. bellina no. 1102-44 was successfully grouped into five resistance classes at 24 JSI (hours after inoculation). A total of three accessions were identified as having resistance to soft rot disease, with two accessions originating from the 22.19 µM media treatment (T2-15 and T2-17) and one other accession originating from the 44.39 µM media treatment (T4-23). Keywords: orchid, Dickeya dadantii, plant resistance, leaf disk technique Abstrak: Anggrek Phalaenopsis merupakan salah satu tanaman hias yang sangat diminati masyarakat karena memiliki warna dan bentuk bunga yang menarik. Salah satu hambatan dalam keberhasilan budidaya tanaman anggrek adalah serangan penyakit busuk lunak yang disebabkan oleh Dickeya dadantii. Oleh karena itu, diperlukan upaya untuk menemukan klon Phalaenopsis yang memiliki ketahanan terhadap penyakit busuk lunak. Penelitian ini bertujuan mengidentifikasi respons ketahanan genotipe anggrek hibrida Phal. ‘Salu Spot’ × Phal. bellina no. 1102-44. Bahan tanaman yang digunakan berupa anggrek hibrida Phal. ‘Salu Spot’ × Phal. bellina no. 1102-44 yang diinokulasi dengan bakteri Dickeya dadantii. Identifikasi respons ketahanan dilakukan melalui metode infeksi menggunakan teknik leaf disk. Hasil penelitian menunjukkan bahwa klon Phal. ‘Salu Spot’ × Phal. bellina no. 1102-44 berhasil dikelompokkan ke dalam lima kelas ketahanan pada 24 JSI (jam setelah inokulasi). Sebanyak tiga aksesi teridentifikasi memiliki ketahanan terhadap penyakit busuk lunak, dengan dua aksesi berasal dari perlakuan media 22,19 µM (T2-15 dan T2-17) dan satu aksesi lainnya berasal dari perlakuan media 44,39 µM (T4-23). Kata kunci: Anggrek, Dickeya dadantii, ketahanan tanaman, tekhnik leaf disk
Studi In Silico Sekuen NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) pada Jeruk yang Terkait dengan Resistensi Huanglongbing Kristianto Nugroho; Tri Joko Santoso; Mia Kosmiatin; Dewi Sukma; Agus Purwito
Buitenzorg: Journal of Tropical Science Vol 2 No 2 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i2.25

Abstract

The evaluation of citrus resistance to Huanglongbing (HLB) disease is still challenging due to the incapability of the bacteria to be cultured purely in artificial medium, the complexity of inoculation methods, and the long duration required for phenotypic observation. Thus, the use of molecular markers is one of the alternatives to solve this problem. The focuses of this study were to perform in silico analysis of the nucleotide variations in NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) gene sequence among several citrus genotypes whose resistance information to HLB have been known previously and to employ phylogenetic analysis among them. The NPR1 gene sequences from 20 genotypes which consisted of 14 citrus and six relative genotypes were collected in silico from Citrus Genome Database and analyzed using multiple sequence alignment program. A total of six interesting SNPs that could distinguish between susceptible and resistant citrus genotypes were detected in this study. As many as five SNPs were non-synonymous, while only one synonymous SNP that did not cause the amino acid change was identified in this study. The phylogenetic analysis also revealed the separation between susceptible and tolerant/resistant citrus genotypes in two main clusters. The SNPs found in this study are expected to be useful for designing new functional markers as a selection tool in future studies. Keywords: in silico, molecular marker, phylogenetic analysis, selection tool, SNP
Co-Authors , Asnawatr , Nurhasanah , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Akhmad Kosasih Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alim Setiawan Slamet Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amrullah, Rizki Abi Anas D Susila Andri Ernawati Andri Martin Anjani, Imas Galuh Antonius Suwanto Arif Satria Arif Satria Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Asep Setiawan Atra Romeida Awang Maharijaya Bambang S. Purwoko Bambang Sapta Purwoko Branco, Luis Manuel Budi Marwoto Budi Marwoto Budi Marwoto Budi Winarto Chaireni Martasari Chaireni Martasari Darda Efendi Dewi Sukma Dewi Sukma Dian Puji Rahayu Didy Soepandi Didy Soepandi, Didy Didy Sopandie Dini Dinarti Diny Dinarti Dwi Andreas Santosa Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih ENDAH RETNO PALUPI ENDAH RETNO PALUPI Endah Retno Palupi Endang Gunawan ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fathur Rahmi, Atika Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Halida Adistya Putri Hartati, Raden Roro Sri Harti, Heri Heriansyah Putra Herison, Catur Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur Iskandar Z Siregar ISKANDAR ZULKARNAEN SIREGAR Ismail Maskromo ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu JANE KATHRYNE JOLANDA LAISINA Juang Gema Kartika Julius D. Nugroho Kalsum Yulifar, Andi Sri Ummi Kartiman, Roni Kartiman, Roni Karyanti ,, Karyanti Kikin H Mutaqin Komang Trisna Wirakusuma Kosasih, Akhmad Kristianto Nugroho Kristianto Nugroho Kristianto Nugroho KRISTIANTO NUGROHO, KRISTIANTO Laela Sari Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH LUIS MANUEL BRANCO M. Herman M. Machmud Marlin Marlin MASKROMO, ISMAIL Mastur Matjik, Nurhayati Anshori MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu MELATI MELATI Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mira Humaira Mohamad Prayogi Mohamad Rahmad Suhartanto Muhamad Herman Muhammad Alwi MUHAMMAD H. ANUGRAH MUHAMMAD HERMAN Muhammad Herman Muhammad Mahmud Muhammad Reza Zakie MUHAMMAD SYUKUR Ni Made Armini Wiendi Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie Nurul Khumaida Nurwita Dewi Prasetyawati , Adinda Prima Muklisa Puji Lestari Purba, Dumaris Priskila Putra, Heriansyah Putri, Halida Adistya RAGAPADMI PURNAMANINGSIH Ragapadmi Purnamaningsih Ratna Trisnawati Ravenska, Nidya Rd. Selvy Handayani Rd. Zainal Frihadian REFLINUR REFLINUR Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati RIZKI ABI AMRULLAH Rizki Abi Amrullah Roedhy Poerwanto Roni Kartiman Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati Rr. Sri Hartati RR. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rudiyanto Rudiyanto Rustikawati S. M. Sumaraow S. M. Sumaraw S. Sudarsono Sari, Dewi Citra Sari, Laela SATRIYAS ILYAS SATRIYAS ILYAS Sembiring, Rinawati SIENTJE MANDANG SUMARAW Sientje Mandang Sumaraw SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Sobir Sobir SOBIR SOBIR Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono, dan SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO Sudirman Yahya Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syaiful Anwar Syarifah Iis Aisyah Tri Joko Santoso Tri Joko Santoso TRI JOKO SANTOSO Tri Joko Santoso Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Warid Willy Bayuardi Suwarno Winarto, dan Budi Wulandari, Dyah Retno Yopi Kurniawan