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Manajemen Panen dan Pasca Panen Edible Flower di Cidadap, Kota Bandung Muhammad Reza Zakie; Diny Dinarti; Agus Purwito
Buletin Agrohorti Vol. 11 No. 1 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i1.46577

Abstract

Kegiatan penelitian telah dilaksanakan di Kecamatan Cidadap, Kota Bandung dari 27 Januari hingga 25 Mei 2019. Tujuan dari pelaksanaan penelitian ini adalah mengetahui permasalahan panen dan pasca panen edible flower di perusahaan dan dapat menemukan cara penanganan pasca panen yang baik. Edible flower memerlukan aspek pasca panen yang tepat agar tetap terjaga kesegaran dan penampilan bunga hingga sampai di tangan konsumen. Aspek teknis yang dilakukan adalah pemasangan instalasi NFT, pembuatan media tanam, persemaian, penanaman, pemeliharaan, panen, dan pasca panen. Aspek yang dipelajari bagian pengemasan akhir edible flower. Pengujian daya simpan pada tiga jenis edible flower Pansy (Viola x wittrockiana), Viola (Viola tricolor), dan Dianthus (Dianthus caryophillus) pada suhu 10 ℃ dan 20 ℃. Ketiga jenis bunga ini dapat mempertahankan kualitas hingga tujuh hari di penyimpanan suhu 10 ℃ hari dibanding dengan suhu 20 ℃ yang hanya bertahan hingga hari ketiga setelah panen. Kata kunci : hidroponik NFT, kualitas edible flowers, penyimpanan
Induksi Proliferasi Tunas Bawang Dayak (Eleutherine americana Merr.) melalui Organogenesis dengan Penambahan IAA dan BAP Ratna Trisnawati; Ni Made Armini Wiendi; Agus Purwito
Buletin Agrohorti Vol. 11 No. 1 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i1.46585

Abstract

Bawang Dayak (Eleutherine americana Merr.) merupakan tumbuhan khas Kalimantan Tengah yang digunakan sebagai obat tradisional oleh suku Dayak. Teknologi kultur jaringan dapat digunakan untuk keperluan budidaya Bawang Dayak dalam menghasilkan kualitas benih yang baik dan ketersediaan benih yang berkelanjutan. Penelitian ini bertujuan mempelajari respon eksplan mata tunas vegetatif dari umbi Bawang Dayak dengan penambahan IAA dan BAP. Penelitian ini diharapkan dapat memperoleh komposisi zat pengatur tumbuh IAA dan BAP yang optimal untuk perbanyakan tanaman Bawang Dayak secara in vitro. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan II, Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor pada bulan Oktober 2018 sampai Juni 2019. Penelitian ini terdiri dari 3 percobaan terpisah yang masing-masing menggunakan jenis eksplan dan perlakuan berbeda setiap percobaan. Percobaan I, proliferasi tunas aseptic umbi Bawang Dayak. Percobaan II, induksi proliferasi tunas dengan penambahan IAA dan BAP. Percobaan III, induksi tunas dari umbi ex vitro Bawang Dayak. Percobaan I, eksplan ditanam pada media perbanyakan KC2 dan MS13K. Percobaan II dan III.a disusun menggunakan rancangan perlakuan faktorial disusun dalam Rancangan Kelompok Lengkap Teracak (RKLT), terdiri dari 2 faktor, yaitu IAA (0.0, 0.5, 1.0, 1.5 mg L-1) dan BAP (0.0, 1.0, 2.0, 3.0 mg L-1). Percobaan III.b terdiri atas 3 faktor, yaitu IAA (0.0, 0.5, 1.0, 1.5 mg L-1), BAP (0.0, 1.0, 2.0, 3.0 mg L-1), dan GA3 (0.0, 1.0, 2.0, 3.0, 4.0 mg L-1). Media perlakuan KC2 memiliki rataan jumlah tunas lebih tinggi sebesar 1.93 tunas per eksplan dibandingkan dengan media MS13K sebesar 1.42 tunas per eksplan. Media perlakuan IAA 1.0 mg L-1 dan media perlakuan BAP 3.0 mg L-1 berpengaruh sangat nyata dalam pembentukan tunas sebesar 6.5 tunas per eksplan. Subkultur kedua, media perlakuan IAA 1.5 mg L-1 berpengaruh sangat nyata dalam pembentukan tunas sebesar 3.3 tunas per eksplan. Media perlakuan IAA 1.5 mg L-1 + BAP 3.0 mg L-1 berpengaruh sangat nyata dalam pembentukan daun sebesar 5.7 daun per eksplan dibandingkan dengan media perlakuan yang lain. Kata kunci: Benzil Amino Purin, eksplan, Indole Acetic Acid, in vitro
Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi Laela Sari; Agus Purwito; Didy Sopandie; Ragapadmi Purnamaningsih; Enny Sudarmonowati
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6612

Abstract

The purpose of this study was to identify the selection criteria to obtain a superior mutant derived from the wheat plants of such varieties as Dewata, Selayar and Alibey, adaptive in medium land. The analysis of agronomic growth characters showed a significantly effect on a growth percentage of the initial growth (8 mutants), flowering time (1 mutant), panicle stem length (15 mutants), number of panicles (7 mutants), the number of grains per panicle (8 mutants), grain weight observed (8 mutants), grain weight per genotype (6 mutants), leaf area (2 mutants) and leaf greenness (5 mutants). The effects on the characters of ripe time, harvest, panicle length and plant height were not significant. The mutants of Dewata, Selayar and Alibey could be selected based on the characters of panicle stem length, number of grains per panicle and grain weight per observation because these characters generated more mutants than the other characters. The correlation analysis between the characters of growth and yield components of wheat mutants showed that the number of grains per panicle was positively correlated with the grain weight observed, while the length of panicle stem was positively correlated with grain weight per genotype, number of panicles and leaf area. Hopefully some mutants produced could adapt to the tropical medium land, thus adding to the diversity of wheat germplasm in Indonesia, thereby reducing the import of wheat to Indonesia.How to CiteSari, L., Purwito, A., Sopandie, D., Purnamaningsih, R. Sudarmonowati, E. (2016). Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi. Biosaintifika: Journal of Biology Biology Education, 8(3), 353-361. 
Etiology of Anthracnose Disease on Shallots Caused by Colletotrichum gloeosporioides Amrullah, Rizki Abi; Wiyono, Suryo; Maharijaya, Awang; Purwito, Agus
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.206-214

Abstract

Penyakit antraknosa dapat terjadi pada seluruh fase pertumbuhan tanaman bawang merah (Allium cepa var. aggregatum) dan belum diketahui proses perkembangannya serta karakteristik penyebabnya. Penelitian dilakukan untuk mempelajari etiologi penyakit antraknosa yang meliputi perkembangan gejala, periode inkubasi dan karakteristik patogen melalui pengujian secara in vivo dan in planta. Awal gejala ialah munculnya bercak putih yang kemudian membesar hingga menyebabkan jaringan mati. Gejala khas yang ditimbulkan ialah adanya pola konsentris berwarna jingga kecokelatan pada bercak dan memiliki periode inkubasi 2-4 hari. Karakteristik morfologi koloni patogen berwarna putih, konidium berbentuk silindris, hifa bersekat. Karakter lain ditandai dengan keberadaan aservulus dan setae. Patogen yang diidentifikasi secara molekuler menunjukkan 99.51% homologi dengan Colletotrichum gloeosporioides aksesi KT390192.1 dari China yang berasal dari tanaman tembakau.
Campus Setting as Living Labs: Lessons from IPB University, Bogor, Indonesia Satria, Arif; Slamet, Alim Setiawan; Kosasih, Akhmad; Purwito, Agus; Siregar, Iskandar Z; Putra, Heriansyah
Journal of Sustainability Perspectives Vol 3: Special Issue 2023
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jsp.2023.20712

Abstract

IPB University in Bogor, Indonesia, has developed its campuses as living laboratories for education, research, community outreach, innovation, and business. The university's infrastructure under the living lab concept, such as forestlands, farmlands, wetlands, teaching factories, and business units, offer real-life opportunities for students and staff to create greater impacts to societies from their academic activities. This paper presents the lessons learned from IPB's campus setting as a living lab, focusing on how students and stakeholders can learn from the existing infrastructure and facilities, how research can benefit from a natural setting, and how the campus can serve as a model for sustainable living. Implementing living lab concept faces challenges as other new initiatives, particularly on the aspects of effective interdisciplinary works, formalizing the community of practices in which pool of experts are organized and knowledge management system is developed. In conclusion, in the era of emancipated learning implementation in Indonesia, IPB University's campus setting could provide a platform for facilitating activities related to science-practices/policy interface.
Campus Setting as Living Labs: Lessons from IPB University, Bogor, Indonesia Satria, Arif; Slamet, Alim Setiawan; Kosasih, Akhmad; Purwito, Agus; Siregar, Iskandar Z; Putra, Heriansyah
Journal of Sustainability Perspectives Vol 3: Special Issue 2023
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jsp.2023.20712

Abstract

IPB University in Bogor, Indonesia, has developed its campuses as living laboratories for education, research, community outreach, innovation, and business. The university's infrastructure under the living lab concept, such as forestlands, farmlands, wetlands, teaching factories, and business units, offer real-life opportunities for students and staff to create greater impacts to societies from their academic activities. This paper presents the lessons learned from IPB's campus setting as a living lab, focusing on how students and stakeholders can learn from the existing infrastructure and facilities, how research can benefit from a natural setting, and how the campus can serve as a model for sustainable living. Implementing living lab concept faces challenges as other new initiatives, particularly on the aspects of effective interdisciplinary works, formalizing the community of practices in which pool of experts are organized and knowledge management system is developed. In conclusion, in the era of emancipated learning implementation in Indonesia, IPB University's campus setting could provide a platform for facilitating activities related to science-practices/policy interface.
Inoculation Methods to Determine Resistance of Phalaenopsis amabilis (L.) Regeneratded from Irradiated Protocorms to Dickeya dadantii Putri, Halida Adistya; Sukma, Dewi; Sudarsono, Sudarsono; Purwito, Agus
PLANTA TROPIKA Vol 12, No 1 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i1.16731

Abstract

Soft-rot disease (SRD) in Phalaenopsis, caused Dickeya dadantii, has resulted in significant losses in the orchid sector in Indonesia. This study aimed to evaluate the inoculation method of Dickeya dadantii and identify the resistance response of individual regenerated plantlets of Phal. amabilis from irradiated protocorm. A detached leaf assay was used to evaluate the inoculation method and resistance response of SRD. Based on the results of this study, Dickeya dadantii bacteria could only infect the leaves through wounding tissue. The density of bacteria that could infect leaf tissue was OD600 = 0.2. All dilution factors tested caused soft rot symptoms in P. amabilis. On the other hand, Vanilla planifolia only showed symptoms at a dilution factor of 10-0. Four accessions of regenerated plantlets from irradiated protocorms were resistant to SRD. They were from irradiation 5 Gy (IP 05 Gy-23, IP 05 Gy-31, and IP 05 Gy-33) and one accession from the control treatment or without irradiation (IP 0 Gy -1). These results showed that 5 Gy irradiation increased plant resistance to SRD in Phalaenopsis. A dose of 5 Gy can potentially produce mutant lines resistant to SRD in Phalaenopsis or other plants, too.
Pollen Dispersal and Pollination Patterns Studies in Pati Kopyor Coconut using Molecular Markers Larekeng, Siti Halimah; Maskromo, Ismail; Purwito, Agus; Matjik, Nurhayati Anshori; Sudarsono, S.
International Coconut Community Journal Vol 31 No 1 (2015): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.111 KB) | DOI: 10.37833/cord.v31i1.70

Abstract

Parentage analysis has been used to evaluate pollen dispersal in Kopyor coconut (Cocos nucifera L.). Investigations were undertaken to elucidate (i) the dispersal of pollen, (ii) the rate of self and out-crossing pollination, and (iii) the distance of pollen travel in Pati kopyor coconut population. The finding of this activities should be beneficial to kopyor coconut farmers to increase their kopyor fruit harvest and to support breeding of this unique coconut mutant. As many as 84 progenies were harvested from 15 female parents. As many as 95 adults coconut provenances surrounding the female parents were analyses as the potential male parents for the progenies. The adult coconut palms were mapped according to their GPS position. All samples were genotyped using six SSR and four SNAP marker loci. Parentage analysis was done using CERVUS version 2.0 software. Results of the analysis indicated that evaluated markers were effective for assigning candidate male parents to all evaluated seedlings. There is no specific direction of donated pollen movement from assigned donor parents to the female ones. The donated pollens could come from assigned male parents in any directions relative to the female parent positions. Cross pollination occured in as many as 82.1% of the progenies analyzed. Outcrossing among tall by tall (TxT), dwarf by dwarf (DxD), hybrid by hybrid (HxH), TxD, DxT, TxH, DxH, and HxD were observed. Self-pollination (TxT and DxD) occurred in as many as 17.9% of the progenies. The dwarf coconut was not always self pollinated. The presence of DxD, TxD, and HxD outcrossing was also observed. The donated pollens could come from pollen donor in a range of at least 0-58 m apart from the evaluated female recipients. Therefore, in addition to the wind, insect pollinators may have played an important role in Kopyor coconut pollination.
Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana Nurita TORUAN-MATHIUS; . LUKMAN; . AGUS-PURWITO
Menara Perkebunan Vol. 74 No. 2: 74 (2), 2006
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v74i2.103

Abstract

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization  for Cinchona plantlets from  micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of  C. ledgeriana  QRC clone were used as  scions, while of C. succirubra as  rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of  combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were  the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived  plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization.  Acclimatization  consisted  of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC  for two weeks,  followed by aclimatization in a plastic house with  70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan  Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan.  Pada tanaman kina teknik sambung mikro  in vitro belum pernah dilakukan. Tujuan penelitian ini adalah  menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung  mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi  medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet  C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu  kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan  dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup,  jumlah daun,  berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung  mikro  yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro.  Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur  suhu 25 -     27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini  90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal   Cinchona sp..
Kompatibilitas sambung mikro Cinchona ledgeriana dengan C. succirubra berdasarkan anatomi dan elektroforesis SDS- PAGE protein daerah pertautan Compatibility of micrografting Chincona ledgeriana and C. succirubra based on anatomy and SDS-PAGE protein electrophoresis of union area Nurita TORUAN-MATHIUS; . LUKMAN; . AGUS - PURWITO
Menara Perkebunan Vol. 75 No. 2: 75 (2), 2007
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v75i2.147

Abstract

SummaryRootstocks and scions interaction causesdifferent responses within individuals of scionfrom the same clone. The objectives of thisresearch were to determine compatible anduncompatible combinations of micrograftingbetween Cinchona succirubra A, andC. succirubra B with C. ledgeriana QRC205and Cib5 clones, based on anatomy structureand SDS-PAGE protein bands pattern ofstem at union area between rootstock andscion. The research was arranged in aCompletely Randomized Design, withrootstock/scion combinations CSA/QRC205,CSA/Cib5, CSB/QRC205, CSB/Cib5 and as acontrols were the combination of CSA/CSA,CSB/CSB, QRC205/QRC205, Cib5/Cib5, succiand ledger seedlings with the same age. Effectof rootstocks on scion was studied based onanatomy structure of the union area betweenrootsocks and scions and SDS-PAGE proteinbands pattern. The results showed that theunion of stem between rootstocks and scionwas initiated by callus formation, cellsdifferentiation and the vascular vesselsformation. The anatomy of stem union area ofCSA/QRC205 as a compatible combination ofrootsotck and scion was the same asunmicrografted plantlet. At uncompatiblecombination CSB/Cib5 showed the formationof stone cells as a line along stem cyrcle atunion area and heavely callus formation atoutside of rootstock and scion stems. SDS-PAGE protein bands pattern from thecompatible combination was the same asplanlet control. On the otherhand, from theuncompatible combinations CSB/Cib5 werefound protein degradation and the formation ofnew proteins with molecular weight 21 and 30kD.RingkasanInteraksi batang bawah dengan batangatas menimbulkan berbagai keragaman responsantar individu batang atas dari klon yang sama.Tujuan penelitian ini adalah untuk me-netapkan kombinasi yang kompatibel denganyang tidak kompatibel antara kina klonCinchona succirubra A, dan C. succirubra Bdengan C. ledgeriana klon QRC205 danCib5, berdasarkan anatomi dan pola pitaSDS-PAGE protein daerah pertautan antarabatang atas dengan batang bawah. Percobaandisusun dengan Rancangan Acak Lengkapkombinasi yang diuji adalah CSA/QRC205,CSA/Cib5, CSB/QRC205, CSB/Cib5 dengankombinasi kontrol CSA/CSA, CSB/CSB,QRC205/QRC205, Cib5/Cib5, tanaman kinasucci dan ledger tanpa sambungan denganumur yang sama. Pengamatan dilakukanterhadap struktur anatomi daerah pertautanantar batang bawah dengan batang atas danpola pita protein SDS-PAGE batang atas. Hasilyang diperoleh menunjukkan bahwa tahapanpemulihan daerah pertautan penyambunganbatang bawah dengan batang atas diawalidengan pembentukan kalus, diferensiasi sel danterbentuknya jaringan ikatan pembuluhgabungan. Kombinasi antar batang bawahdengan batang atas yang kompatibel yaituCSA/QRC205 memperlihatkan strukturanatomi daerah pertautan yang serupa denganstruktur anatomi batang planlet yang tidakdisambung. Pada kombinasi yang tidak kom-patibel yaitu CSB/Cib5 pada daerah pertautanterbentuk sel-sel batu berbentuk garis yangmemanjang di tengah lingkaran batang. Disamping itu pada daerah pertautan terbentukkalus yang berlebih ke arah luar baik padabatang atas maupun batang bawah. Padakombinasi yang kompatibel pola pita proteinsama dengan planlet kontrol. Pada kombinasiyang tidak kompatibel yaitu kombinasiCSB/Cib5 terjadi degradasi protein danpembentukan protein baru dengan beratmolekul sekitar 21dan 30 kD
Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amrullah, Rizki Abi Anas D Susila Andri Ernawati Anjani, Imas Galuh Antonius Suwanto Arif Satria Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atra Romeida Awang Maharijaya Bambang S. Purwoko Bambang Sapta Purwoko Branco, Luis Manuel Budi Marwoto Budi Marwoto Budi Winarto Catur Herison Chaireni Martasari Dewi Sukma Dian Puji Rahayu Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih Endah Retno Palupi Endang Gunawan ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fathur Rahmi, Atika Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Harti, Heri Herison, Catur Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISKANDAR ZULKARNAEN SIREGAR ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Juang Gema Kartika Julius D. Nugroho Kalsum Yulifar, Andi Sri Ummi Kartiman, Roni Kartiman, Roni Karyanti ,, Karyanti Kikin H Mutaqin Komang Trisna Wirakusuma Kosasih, Akhmad KRISTIANTO NUGROHO, KRISTIANTO Laela Sari Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Maharijaya, dan Awang Marlin MASKROMO, ISMAIL Mastur Mastur Matjik, Nurhayati Anshori MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi MUHAMMAD HERMAN Muhammad Herman Muhammad Mahmud Muhammad Reza Zakie MUHAMMAD SYUKUR Ni Made Armini Wiendi Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie Nurul Khumaida Nurwita Dewi Prasetyawati , Adinda Prima Muklisa PUJI LESTARI Purba, Dumaris Priskila Putra, Heriansyah Putri, Halida Adistya Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Trisnawati Ravenska, Nidya Rd. Selvy Handayani Rd. Zainal Frihadian REFLINUR REFLINUR Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah Roedhy Poerwanto Roni Kartiman Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati RR. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo S. M. Sumaraow S. M. Sumaraw S. Sudarsono Sari, Dewi Citra Sari, Laela SATRIYAS ILYAS Sembiring, Rinawati SIENTJE MANDANG SUMARAW Sientje Mandang Sumaraw SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Slamet, Alim Setiawan Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono, dan SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO Sudirman Yahya Suhartanto , M. Rahmad Suhartanto, Muhammad Rahmad Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syaiful Anwar Syarifah Iis Aisyah TRI JOKO SANTOSO Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Warid Willy Bayuardi Suwarno Winarto, dan Budi Wulandari, Dyah Retno Wulandari, Dyah Retno Yopi Kurniawan