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Karakter Agro-morfologi dan Periode After-ripening Benih Padi Lanras Lokal Potensial asal Timor-Leste Branco, Luis Manuel; Ilyas, Satriyas; Purwoko, Bambang Sapta; Agus Purwito; Palupi, Endah Retno
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 3 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.498 KB) | DOI: 10.24831/jai.v50i3.43102

Abstract

Benih padi yang baru dipanen umumnya tidak berkecambah walaupun dikecambahkan pada kondisi optimum yang disebut after-ripening, dan secara alamiah akan hilang setelah waktu tertentu. Penelitian ini bertujuan melakukan karakterisasi agro-morfologi dan menetapkan periode after-ripening benih 5 lanras potensial di Timor-Leste. Penelitian dilaksanakan pada bulan April 2018-November 2019. Penanaman dan pengamatan karakter agro-morfologi 5 lanras potensial Timor-Leste, yaitu Hare R-oitu, Fos Mean, Hare Belit, Hare Nona Portu, dan Ale Mamea Ula Lesa, dilaksanakan di Desa Caibada Distrik Baucau, Timor-Leste. Pengamatan periode after ripening dan mutu benih ke lima lanras tersebut dilaksanakan di Laboratorium Pengujian dan Penyimpanan Benih, Departemen Agronomi dan Hortikultura, IPB University, dan dirancang dalam rancangan acak lengkap (RAL) dua faktor dengan empat ulangan. Faktor pertama adalah 5 lanras tersebut di atas dan faktor kedua adalah periode simpan yang terdiri atas 2, 3, 4, 5, 6, 7, dan 8 minggu setelah panen (MSP). Hasil penelitian menunjukkan bahwa 5 lanras potensial di Tinmor-Leste mempunyai keragaan tanaman yang pendek (76.3-105.50 cm), tergolong berumur sedang (129-141 HSS), dan jumlah anakan produktif tinggi (17.5-53.50 anakan), kecuali Ale Mamea Ula Lesa. Lima lanras tersebut mempunyai potensi produktivitas yang tinggi berkisar 8.1-9.8 ton ha-1. Periode after-ripening lanras Hare Belit dan Hare Nona Portu berakhir pada 2 MSP, Hare R-oitu pada 6 MSP, sedangkan Ale Mamea Ula Lesa dan Fos Mean pada 8 MSP. Semakin lama benih disimpan rasio kandungan ABA/GA3 semakin menurun dari 0.53-1.22 ppm pada 2 MSP menjadi 0.45-0.93 ppm pada 4 MSP. Lanras Hare Belit dan Hare Nona Portu merupakan lanras yang potensial dikembangkan menjadi varietas nasional di Timor-Leste, masing-masing untuk pengembangan di dataran rendah dan dataran sedang. Kata kunci: karakterisasi, intensitas dormansi, persentase perkecambahan, mutu benih
Determination of anthracnose (Colletotrichum gloeosporioides) resistance group in shallot (Allium cepa var. aggregatum) Rizki Abi Amrullah; Maharijaya, Awang; Purwito, Agus; Wiyono, Suryo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 3 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v51i3.47429

Abstract

Shallot anthracnose, caused by Colletotrichum gloeosporioides, is a devastating disease in a tropical country with high humidity and rainfall. Chemical control of anthracnose is neither economical nor eco-friendly, and genetic resistance is considered an efficient management method. This study aimed to determine the resistance groups of several shallot varieties and predict resistance heritability characteristics. In this study, a total of 13 Indonesian shallot varieties were evaluated for anthracnose resistance and separated into two groups, resistance and susceptible, based on K-means clustering developed by using disease resistance/susceptibility characteristics such as incubation period, disease incidence, disease severity, and spot diameter. The results indicate that the Agrihorti, Maja Cipanas, Batu Ijo, and Rubaru varieties were identified as resistant groups based on lower disease severity and incidence, smaller spot diameter, and longer incubation period. Maja Cipanas and Rubaru were more consistent in all variables, which is recommended as a source of genetic resistance genotypes. On the other hand, the Biru Lancor, Bima Brebes, Srikayang, Violetta, Slupu Merah, Pancasona, Sakato, Katumi, and Kuning varieties were identified as susceptible groups based on high disease severity and incidence, large spot diameter, and short incubation period. Keywords: susceptibility characteristics, genetic resistance, k-means clustering, disease severity
Seed tuber production of potato from stem cuttings, planting densities, and paclobutrazol concentrations Kalsum Yulifar, Andi Sri Ummi; Maharijaya, Awang; Purwito, Agus; Gunawan, Endang; Harti, Heri; Suhartanto , M. Rahmad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 1 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i1.52685

Abstract

Rapid multiplication of potato seed using cuttings is a pivotal strategy for ensuring seed availability. This study aimed to elucidate the interactive effects of node number, paclobutrazol concentration, and planting density on seed potato and cutting productions. The study consisted of two experiments. The first experiment evaluated two genotypes (Granola and PKHT-6) with one or two nodes per cutting. The second experiment investigated planting density (one, three, or five plants per polybag) and paclobutrazol concentration (0, 15, 30, and 45 mg L-1). Notably, node number did not significantly influence cutting success, despite genotype-specific and interactive effects on vegetative parameters like plant height, stem diameter, leaf number, and root length. Planting density had no statistically significant impact on all vegetative parameters but demonstrably affected all production parameters. Conversely, paclobutrazol concentration significantly affected all vegetative parameters and influenced all production parameters except tuber weight per plant. From the results of this research, the use of single cutting is more recommended because it will obtain more plant material compared to two-node cuttings. Thus, in a more global context of potato development, this has the potential to increase the production of potato seeds from cuttings twice as much as the current seed production. The use of three plants/polybags is more recommended since it will produce more tuber but not different with five plants, and the use of paclobutrazol 15 mg L-1 is also recommended to increase the number of tubers. In this way, the production costs of G0 potato seeds can be reduced so that the availability of G0 potato seeds will be more guaranteed and affordable. Keywords: Granola; PKHT-6; rapid multiplication; tuber production
Transformation of Ponkan Mandarin (Citrus reticulata Blanco) by CRISPR/Cas-9-gRNA-CsCS to increase plant resistance to huanglongbing disease Dian Puji Rahayu; Purwito, Agus; Kosmiatin, Mia; Husni, Ali
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 2 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i2.53465

Abstract

The gene that regulates callose could be modified by CRISPR/Cas9 technology. This research aimed to insert the CRISPR/Cas9-CsCS gene into Ponkan orange genome using Agrobacterium tumefaciens. The explants were soaked in a bacterial suspension for 20 minutes and incubated for 2-3 days. In vitro acceleration growth was conducted with a two-factor completely randomized design. The first factor is the type of explant with three levels (nucellar embryo, zygotic embryo, cotyledon node), and the second factor is the type of media with 6 levels (VMW, MT, MSK0, MSK1, MSK2, MSK3). The results showed of all explant types, the highest plant height average and number of leaves were obtained in a media combination of MS + Kinetin 2 mg L-1, MT, and MS + Kinetin 3 mg L-1. The highest transformation efficiency was in the nucellar embryo explant, while the highest regeneration efficiency was in the zygotic embryo explant. The highest shoot tip grafting percentage was achieved in the cotyledon node explant at 100%. In the grafting phase, the putative transformants before and after artificial bacterial inoculation showed that Ponkan 606 and Ponkan 597 had the highest plant heights, respectively. The intensity of Huanglongbing attacks after bacterial inoculation showed that three genotypes did not show HLB symptoms in the 24th week of observation, namely genotypes 598, 606, and 607. This study concluded that gene transformation in citrus plants produced three genotypes that did not show HLB symptoms. Keywords: transgenic; Agrobacterium tumefaciens; Citrus Vein Phloem Degeneration (CVPD)
Pengembangan Metode Devigorasi dengan Pengusangan Cepat untuk Menduga Vigor Daya Simpan Benih Jagung (Zea mays L.) Anjani, Imas Galuh; Suhartanto, Muhammad Rahmad; Purwito, Agus
Buletin Agrohorti Vol. 11 No. 3 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i3.48432

Abstract

Penurunan mutu benih atau deteriorasi merupakan salah satu penghambat dalam produksi benih jagung dan umumnya terjadi selama penyimpanan benih yang ditandai dengan turunnya viabilitas dan vigor benih sehingga mengakibatkan jeleknya pertanaman serta menurunnya hasil. Pengusangan cepat secara kimia merupakan salah satu metode yang digunakan untuk mengetahui percepatan penurunan kualitas benih dengan memanfaatkan kinerja uap etanol. Pengusangan ini dilakukan oleh APC IPB 77-1 MM yang berfungsi melakukan devigorasi benih baik secara fisik dengan uap panas maupun secara kimia dengan etanol. Hasil dari pengujian ini akan digunakan untuk menduga vigor daya simpan (VDS) benih jagung sehingga pemanfaatan dan pengolahan benih jagung dapat dilakukan secara efektif dan efisien. Penelitian dilaksanakan di Laboratorium Biologi dan Biofisika Benih, Laboratorium Penyimpanan dan Pengujian Mutu Benih, Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor dari bulan Maret 2019 hingga Agustus 2019. Penelitian ini terdiri dari dua percobaan. Percobaan pertama yaitu metode pengusangan dengan menyimpan benih pada kelembaban 98% dan suhu 40 oC selama 10 hari. Rancangan yang digunakan adalah Rancangan Kelompok Lengkap Teracak (RKLT) dengan satu faktor yaitu varietas jagung. Percobaan kedua yaitu pengusangan cepat benih jagung secara kimia dengan etanol 96% menggunakan APC IPB 77-1 MM. Rancangan yang digunakan adalah RKLT satu faktor varietas jagung. Hasil percobaan menunjukkan alat pengusangan cepat IPB 77-1 MM dapat digunakan untuk menduga penurunan vigor daya simpan pada benih jagung. Pengujian pengusangan cepat secara kimiawi lebih efektif dan efisien dalam menentukan nilai vigor daya simpan karena membutuhkan waktu yang relatif lebih singkat. Hasil perbandingan secara deskriptif menunjukkan bahwa APC IPB 77-1 MM dapat menunjukkan urutan nilai vigor daya simpan benih kelima varietas jagung yang sama dengan percobaan pengusangan dengan metode penyimpanan pada suhu dan kelembaban tinggi. Kata kunci: Alat Pengusang Cepat IPB 77-1 MM, etanol, kemunduran benih, penyimpanan terkonrol
Pengelolaan Pemupukan di Perkebunan Pisang Cavendish Klon DM2 Lampung Timur, Lampung Anwar, Syaiful; Dinarti, Diny; Purwito, Agus
Buletin Agrohorti Vol. 11 No. 3 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i3.48440

Abstract

Pisang merupakan buah tropis yang sangat diminati oleh masyarakat Indonesia, sehingga dibutuhkan peningkatan produksi buah pisang yang tinggi dengan kualitas yang baik melalui manajemen pemupukan. Kegiatan penelitian bertujuan mempelajari pengelolaan pemupukan pisang Cavendish klon DM2 di Lampung Timur. Penelitian dilaksanakan pada Januari hingga Mei 2019. Pengamatan pengelolaan pemupukan meliputi konsep 5T (tepat jenis, tepat dosis, tepat waktu, tepat cara, dan tepat tempat) dan evaluasi produksi. Analisis data hasil penelitian menggunakan Uji t-student. Hasil pengamatan di areal dibandingkan dengan standar dari Departemen Research and Development. Pemupukan di kebun telah memenuhi kriteria tepat kerja. Ketepatan realisasi dosis pupuk Urea, Kiesrite, dan ZnSO4 telah tepat dosis, sedangkan pupuk KCl dan Petro cas kurang tepat dosis. Realisasi waktu pemupukan belum memenuhi standar kebun. Ketepatan cara pemupukan Urea, KCl, dan Petro cas telah memenuhi kriteria, namun Kiestrite dan ZnSO4 belum memenuhi standar kebun. Semua jenis pupuk belum tepat tempat saat aplikasi kecuali pupuk ZnSO4. Umur buah rata-rata yang dipanen pada sampel blok pengamatan belum maksimal dengan lebar buah yang masih di bawah standar kebun. Jumlah sisir dan hasil bobot tandan juga masih lebih rendah dibandingkan standar kebun. Kata kunci: pisang, pupuk, produksi, tandan
Produksi Tanaman Anyelir (Dianthus chinensis L.) sebagai Tanaman Hias Pot dan Hamparan di Cianjur, Jawa Barat Prasetyawati , Adinda; Kartika, Juang Gema; Purwito, Agus
Buletin Agrohorti Vol. 12 No. 1 (2024): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v12i1.48533

Abstract

Pembibitan tanaman hias biasanya menghasilkan banyak varietas anyelir. Keragaman morfologi antar varietas (dalam satu spesies) memberikan keleluasaan bagi konsumen dalam memilih produk yang akan dibeli. Penelitian bertujuan untuk memperoleh informasi mengenai produksi, kriteria panen komoditas anyelir serta mengevaluasi kelayakan usaha tani dari komoditas tersebut. Penelitian dilakukan di Cianjur, Jawa Barat, Indonesia, pada bulan Januari hingga April 2020. Terdapat 2 percobaan, yaitu (1) mengamati perbedaan 2 varietas anyelir yang diproduksi sebagai tanaman hias pot; varietas anyelir yang diamati memiliki kode DNT 417 dan DNT 917; dan (2) mengamati perbedaan 2 varietas anyelir yang diproduksi dalam polibag untuk digunakan sebagai tanaman hamparan, varietas anyelir yang diamati memiliki kode DNT 103 dan DNT 123. Secara umum keempat varietas yang diamati (untuk anyelir pot dan polibag) yang diproduksi dengan menggunakan prosedur standar perusahaan dapat memenuhi standar mutu yang ditetapkan perusahaan. Nilai R/C ratio pada tanaman anyelir pot dan anyelir polibag yaitu 3.47 dan 2.53. Nilai R/C ratio pada komoditas anyelir pot dan anyelir polibag menunjukkan hasil >1 yang berarti usahatani layak dijalankan dan bersifat menguntungkan. Kata kunci: analisis usaha tani, Caryophyllaceae, kriteria panen, R/C ratio
Analisis Ketahanan Klonal Anggrek Phalaenopsis Terhadap Bakteri Dickeya Dadantii Humaira, Mira; Purwito, Agus; Sudarsono, Sudarsono; Sukma, Dewi
JOURNAL OF SCIENCE AND SOCIAL RESEARCH Vol 8, No 4 (2025): November 2025
Publisher : Smart Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54314/jssr.v8i4.4857

Abstract

Abstract: Phalaenopsis orchids are one of the ornamental plants that are very popular with the public because they have attractive flower colors and shapes. One of the obstacles in the success of orchid cultivation is the attack of soft rot disease caused by Dickeya dadantii. Therefore, efforts are needed to find Phalaenopsis clones that are resistant to soft rot disease. This study aims to identify the resistance response of the hybrid orchid genotype Phal. Salu Spot Phal. bellina no. 1102-44. The plant material used was the hybrid orchid Phal. Salu Spot Phal. bellina no. 1102-44 inoculated with Dickeya dadantii bacteria. Identification of the resistance response was carried out through the infection method using the leaf disk technique. The results showed that the clone Phal. Salu Spot Phal. bellina no. 1102-44 was successfully grouped into five resistance classes at 24 JSI (hours after inoculation). A total of three accessions were identified as having resistance to soft rot disease, with two accessions originating from the 22.19 M media treatment (T2-15 and T2-17) and one other accession originating from the 44.39 M media treatment (T4-23).Keywords: orchid, Dickeya dadantii, plant resistance, leaf disk technique Abstrak: Anggrek Phalaenopsis merupakan salah satu tanaman hias yang sangat diminati masyarakat karena memiliki warna dan bentuk bunga yang menarik. Salah satu hambatan dalam keberhasilan budidaya tanaman anggrek adalah serangan penyakit busuk lunak yang disebabkan oleh Dickeya dadantii. Oleh karena itu, diperlukan upaya untuk menemukan klon Phalaenopsis yang memiliki ketahanan terhadap penyakit busuk lunak. Penelitian ini bertujuan mengidentifikasi respons ketahanan genotipe anggrek hibrida Phal. Salu Spot Phal. bellina no. 1102-44. Bahan tanaman yang digunakan berupa anggrek hibrida Phal. Salu Spot Phal. bellina no. 1102-44 yang diinokulasi dengan bakteri Dickeya dadantii. Identifikasi respons ketahanan dilakukan melalui metode infeksi menggunakan teknik leaf disk. Hasil penelitian menunjukkan bahwa klon Phal. Salu Spot Phal. bellina no. 1102-44 berhasil dikelompokkan ke dalam lima kelas ketahanan pada 24 JSI (jam setelah inokulasi). Sebanyak tiga aksesi teridentifikasi memiliki ketahanan terhadap penyakit busuk lunak, dengan dua aksesi berasal dari perlakuan media 22,19 M (T2-15 dan T2-17) dan satu aksesi lainnya berasal dari perlakuan media 44,39 M (T4-23).Kata kunci: Anggrek, Dickeya dadantii, ketahanan tanaman, tekhnik leaf disk
Studi In Silico Sekuen NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) pada Jeruk yang Terkait dengan Resistensi Huanglongbing Kristianto Nugroho; Tri Joko Santoso; Mia Kosmiatin; Dewi Sukma; Agus Purwito
Buitenzorg: Journal of Tropical Science Vol 2 No 2 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i2.25

Abstract

The evaluation of citrus resistance to Huanglongbing (HLB) disease is still challenging due to the incapability of the bacteria to be cultured purely in artificial medium, the complexity of inoculation methods, and the long duration required for phenotypic observation. Thus, the use of molecular markers is one of the alternatives to solve this problem. The focuses of this study were to perform in silico analysis of the nucleotide variations in NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) gene sequence among several citrus genotypes whose resistance information to HLB have been known previously and to employ phylogenetic analysis among them. The NPR1 gene sequences from 20 genotypes which consisted of 14 citrus and six relative genotypes were collected in silico from Citrus Genome Database and analyzed using multiple sequence alignment program. A total of six interesting SNPs that could distinguish between susceptible and resistant citrus genotypes were detected in this study. As many as five SNPs were non-synonymous, while only one synonymous SNP that did not cause the amino acid change was identified in this study. The phylogenetic analysis also revealed the separation between susceptible and tolerant/resistant citrus genotypes in two main clusters. The SNPs found in this study are expected to be useful for designing new functional markers as a selection tool in future studies. Keywords: in silico, molecular marker, phylogenetic analysis, selection tool, SNP
Molecular diversity of citrus genotypes using callose synthase 7 gene markers linked to Huanglongbing resistance Kristianto Nugroho; Agus Purwito; Dewi Sukma; Mia Kosmiatin; Tri Joko Santoso; Ali Husni; Chaireni Martasari; Puji Lestari
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.64952

Abstract

Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus, is a notable disease affecting citrus plantations globally. Several studies showed that the callose synthase 7 gene is crucial for the citrus defense system against this pathogen. The study aimed to analyze the nucleotide variations and genetic diversity among several citrus genotypes using specific gene primers designed from the callose synthase 7 gene sequence. Genomic DNA from eleven citrus genotypes was amplified using the specific primers, and Sanger sequencing was employed to identify the nucleotide sequence of the PCR products. The results revealed a total of 66 single-nucleotide polymorphisms (SNPs), 10 insertions, and 11 deletions were detected in callose synthase 7 gene fragment sequences. Of these, one out of five noteworthy SNPs identified at a position of 200 bp downstream of the START codon showed distinguishing features between susceptible and resistant/tolerant genotypes. Phylogenetic analysis clearly discriminated the eleven citrus genotypes into two clusters at a dissimilarity coefficient of 0.05, with all genotypes grouped in the first cluster, except for the Chinese box orange and orange jasmine. The identification of notable SNPs in this study can aid in developing new markers for the rapid selection of genotypes with enhanced HLB resistance in citrus breeding programs.   Keywords: Candidatus Liberibacter asiaticus; nucleotide variations; Sanger sequencing; phylogenetic analysis; SNP
Co-Authors , Asnawatr , Nurhasanah , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Akhmad Kosasih Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alim Setiawan Slamet Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amrullah, Rizki Abi Anas D Susila Andri Ernawati Andri Martin Anjani, Imas Galuh Antonius Suwanto Arif Satria Arif Satria Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Asep Setiawan Atra Romeida Awang Maharijaya Bambang S. Purwoko Bambang Sapta Purwoko Branco, Luis Manuel Budi Marwoto Budi Marwoto Budi Marwoto Budi Winarto Chaireni Martasari Chaireni Martasari Darda Efendi Dewi Sukma Dewi Sukma Dian Puji Rahayu Didy Soepandi Didy Soepandi, Didy Didy Sopandie Dini Dinarti Diny Dinarti Dwi Andreas Santosa Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih ENDAH RETNO PALUPI ENDAH RETNO PALUPI Endah Retno Palupi Endang Gunawan ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fathur Rahmi, Atika Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Halida Adistya Putri Hartati, Raden Roro Sri Harti, Heri Heriansyah Putra Herison, Catur Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur Iskandar Z Siregar ISKANDAR ZULKARNAEN SIREGAR Ismail Maskromo Ismail Maskromo ISMAIL MASKROMO Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu JANE KATHRYNE JOLANDA LAISINA Juang Gema Kartika Julius D. Nugroho Kalsum Yulifar, Andi Sri Ummi Kartiman, Roni Kartiman, Roni Karyanti ,, Karyanti Kikin H Mutaqin Komang Trisna Wirakusuma Kosasih, Akhmad Kristianto Nugroho Kristianto Nugroho Kristianto Nugroho KRISTIANTO NUGROHO, KRISTIANTO Laela Sari Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH LUIS MANUEL BRANCO M. Herman M. Machmud Marlin Marlin MASKROMO, ISMAIL Mastur Matjik, Nurhayati Anshori MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu MELATI MELATI Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mira Humaira Mohamad Prayogi Mohamad Rahmad Suhartanto Muhamad Herman Muhammad Alwi MUHAMMAD H. ANUGRAH MUHAMMAD HERMAN Muhammad Herman Muhammad Mahmud Muhammad Reza Zakie MUHAMMAD SYUKUR Ni Made Armini Wiendi Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie Nurul Khumaida Nurwita Dewi Prasetyawati , Adinda Prima Muklisa Puji Lestari Purba, Dumaris Priskila Putra, Heriansyah Putri, Halida Adistya Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Trisnawati Ravenska, Nidya Rd. Selvy Handayani Rd. Zainal Frihadian REFLINUR REFLINUR Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati RIZKI ABI AMRULLAH Rizki Abi Amrullah Roedhy Poerwanto Roni Kartiman Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati RR. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rudiyanto Rudiyanto Rustikawati S. M. Sumaraow S. M. Sumaraw S. Sudarsono Sari, Dewi Citra Sari, Laela SATRIYAS ILYAS SATRIYAS ILYAS Sembiring, Rinawati SIENTJE MANDANG SUMARAW Sientje Mandang Sumaraw SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Sobir Sobir SOBIR SOBIR Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono, dan SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO Sudirman Yahya Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syaiful Anwar Syarifah Iis Aisyah Tri Joko Santoso Tri Joko Santoso Tri Joko Santoso TRI JOKO SANTOSO Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Warid Willy Bayuardi Suwarno Winarto, dan Budi Wulandari, Dyah Retno Yopi Kurniawan