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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Agrotrop : Journal on Agriculture Science Zuriat Jurnal Natur Indonesia Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Aplikasi Bisnis dan Manajemen (JABM) E-Journal Widyariset Planta Tropika JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Biologi Tropis ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science JOURNAL OF SCIENCE AND SOCIAL RESEARCH axial : jurnal rekayasa dan manajemen konstruksi Menara Perkebunan Sainstek : Jurnal Sains dan Teknologi CORD Jurnal Penelitian Tanaman Industri (Littri) Buletin Palma Widyariset Journal Of Sustainability Perspectives Annales Bogorienses

Agus Purwito

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian IPB Jalan Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 350191

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Analisis Ketahanan Klonal Anggrek Phalaenopsis Terhadap Bakteri Dickeya Dadantii Humaira, Mira; Purwito, Agus; Sudarsono, Sudarsono; Sukma, Dewi
JOURNAL OF SCIENCE AND SOCIAL RESEARCH Vol 8, No 4 (2025): November 2025
Publisher : Smart Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54314/jssr.v8i4.4857

Abstract: Phalaenopsis orchids are one of the ornamental plants that are very popular with the public because they have attractive flower colors and shapes. One of the obstacles in the success of orchid cultivation is the attack of soft rot disease caused by Dickeya dadantii. Therefore, efforts are needed to find Phalaenopsis clones that are resistant to soft rot disease. This study aims to identify the resistance response of the hybrid orchid genotype Phal. Salu Spot Phal. bellina no. 1102-44. The plant material used was the hybrid orchid Phal. Salu Spot Phal. bellina no. 1102-44 inoculated with Dickeya dadantii bacteria. Identification of the resistance response was carried out through the infection method using the leaf disk technique. The results showed that the clone Phal. Salu Spot Phal. bellina no. 1102-44 was successfully grouped into five resistance classes at 24 JSI (hours after inoculation). A total of three accessions were identified as having resistance to soft rot disease, with two accessions originating from the 22.19 M media treatment (T2-15 and T2-17) and one other accession originating from the 44.39 M media treatment (T4-23).Keywords: orchid, Dickeya dadantii, plant resistance, leaf disk technique Abstrak: Anggrek Phalaenopsis merupakan salah satu tanaman hias yang sangat diminati masyarakat karena memiliki warna dan bentuk bunga yang menarik. Salah satu hambatan dalam keberhasilan budidaya tanaman anggrek adalah serangan penyakit busuk lunak yang disebabkan oleh Dickeya dadantii. Oleh karena itu, diperlukan upaya untuk menemukan klon Phalaenopsis yang memiliki ketahanan terhadap penyakit busuk lunak. Penelitian ini bertujuan mengidentifikasi respons ketahanan genotipe anggrek hibrida Phal. Salu Spot Phal. bellina no. 1102-44. Bahan tanaman yang digunakan berupa anggrek hibrida Phal. Salu Spot Phal. bellina no. 1102-44 yang diinokulasi dengan bakteri Dickeya dadantii. Identifikasi respons ketahanan dilakukan melalui metode infeksi menggunakan teknik leaf disk. Hasil penelitian menunjukkan bahwa klon Phal. Salu Spot Phal. bellina no. 1102-44 berhasil dikelompokkan ke dalam lima kelas ketahanan pada 24 JSI (jam setelah inokulasi). Sebanyak tiga aksesi teridentifikasi memiliki ketahanan terhadap penyakit busuk lunak, dengan dua aksesi berasal dari perlakuan media 22,19 M (T2-15 dan T2-17) dan satu aksesi lainnya berasal dari perlakuan media 44,39 M (T4-23).Kata kunci: Anggrek, Dickeya dadantii, ketahanan tanaman, tekhnik leaf disk

Embriogenesis Somatik dari Eksplan Daun Anggrek Phalaenopsis sp L. (Somatic Embryogenesis from Leaf Eksplant of Phalaenopsis Orchids) Sri Rianawati; Agus Purwito; Ridho Kurniati; Budi Marwoto; Suryanah Suryanah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Somatic embryogenesis has been recoqnized as one of the process on plant micropropagation techniques. This process occured through regeneration by direct embryo formation and through an intermediary callus phase. This research was conducted through an intermediary callus phase. The experiment was initiated with callus induction from leaf explant on five modifications of MS medium i.e :1/2MS without plant hormone (MI-0); ½ MS containing 1mg/L BA + 0.5 mg/L 2.4-D + 1mg/L NAA (MI-1);1/3 MS containing 2 mg/L 2.4-D (MI-2); ½ MS supplemented with 0.5 mg/L 2.4-D + 0.5 mg/L BAP +0.2 mg/L thidiazuron (MI-3); ½ MS supplemented 2 mg/L thidiazuron and 1 mg/L BAP (MI-4). After the tissues were swollen, the explants were placed on callus proliferation medium ½ MS supplemented with 0.2 mg/L thidiazuron and 0.5 mg/L 2.4-D (MP). After two months, calli were regenerated in regeneration medium ½ MS supplemented with 0.4 mg/L BAP and 0.2 mg/L 2.4-D (MR). The results of this research showed that MI-1 and MI-3 were the best swelling explant mediums before the callus produced in both MP and MR medium. Callus produced was increased in every subculture. However, the level of callii production decreased on the following subculture. Plantlets were regenerated from somatic embryos derived from callii on MR medium. The results of this study may contribute to our advancement of scientific knowledge achievements tissue culture techniques to support inconventional plant improvement. Key words: embryo somatic induction, in vitro, embryogenic callii

Pembentukan Tanaman Cabai Haploid Melalui Induksi Ginogenesis dengan Menggunakan Serbuk Sari yang Diradiasi Sinar Gamma , Suharsono; Muhammad Alwi; Agus Purwito
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 2 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this research was to obtain haploid hot pepper plants by inducing gynogenesis with g-ray irradiated pollen for pollination. Fruits resulted from pollination by irradiated pollen was used as the explants of the in vitro culture in solid MS medium containing BAP, IAA and GA3. The resulted plants then cultivated in MS0 medium. The result of the research showed that hot pepper immature embryo could grow and developed into whole plant when the age of this embryo was 9 days after pollination or more. The haploid hot pepper plants can be obtained by pollinating the pistil with 10 Gy irradiated pollen. The MS medium containing BAP 0.3 + IAA 0.2 + GA3 0.5 mg/l and BAP 0.4 + IAA 0.1 + GA3 0.5 mg/l supported well the development of immature embryo into whole plants. The growth of haploid hot pepper plants was slower than that of diploid ones. Key words: Hot pepper, haploid plant, gynogenesis, g-ray.

Studi RegeDerasi daD Produksi Protoplas Mesofil Daun Beberapa KlOD TanamaD Kentang (Solanum tuberosum L.) . , Asnawatr; G. A. Wattimenal; M. Machmud; Agus Purwito
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 30 No. 3 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objectives of these experiments were to obtain medium composition to enlarge leaf size for protoplast production and to obtain medium composition for plant regeneration. The result showed that the best medium to produce the larger leaves was medium MS with double concentration of macronutrients without hormone supplemented with Morel vitamins, 3% (w/v) sucrose and. 7 g/l agar. This medium produced leaves with diameter of 1.44 cm comparing to control medium MS with 0.67 cm in diameter. Medium MS containing 0.1 mg/l 1AA, 0.5 mg/l Zeatin and 0.5 mg/l GA3 was able to regenerate vigorous shoots of 7 clones. Protoplast isolation of 5 clones using enzyme composition containing 0.5 % cellulase Onozuka RS, 0.05 %, pectolyase Y-23, 0.05 % MES, 9.1 % mannitol and pH 5.5, without CP W medium produced protoplast with variable yield from 10.50 x 1oJ protoplast/g leaves for Atlantic to 46.58x 1oJ protoplast/gforBF15. Keyword: Leaves size, Plant regeneration, Potato, Protoplast i

Transformasi Genetik Tanaman Kentang cv. Atlantik Dengan Mengintroduksikan Gen Hordothionin untuk Mendapatkan Ketahanan terhadap Penyakit Bakteri , Nurhasanah; G. A. Wattimena; Agus Purwito; Ni Made Armini Wiendi; , Suharsono
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 31 No. 2 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Hordothionins are small anti-bacteria proteins present in barley endosperm, To reveal the potential of this proteins for engineering bacterial disease resistance into potato, a semi-synthetic hordothionin gene construct was introduced in potato cv. Atlantic via Agrobacterium tumefaciens strain LBA 4404, under the control of a cauliflower mosaic virus (CaMV) 358 promotor. The in vitro grown stem (internodus) was used in this research. After 6 weeks in regeneration medium and 2 weeks in rooting medium there were 22 regenerated plants that were screened in kanamycine containing medium. PCR analysis using spesific primer from CaMV 358 promotor showed the presence of amplified T-DNA in 4 transgenic lines from 22 putative transgenic plants were tested, The in vitro toxicity against Ralstonia solanacearum tested from transgenic lines showed variation in resistance level, There were only 2 of the transgenic lines were tolerant, while one of them was moderate tolerant even one of them was susceptible. Key words: Potato, Hordothionin gene, Disease resistance

Perbanyakan Tunas Mikro Pisang Rajabulu (Musa AAB Group) dengan Eksplan Anakan dan Jantung Andri Ernawati; Agus Purwito; J. M. Pasaribu
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 2 (2005): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Research on micropropagation of banana cv. Rajabulu (Musa AAB Group) was undertaken. On initiation stage, sucker was best used as explant during rainy season on solid medium containing 7 mg/l BAP + 3 mg/l IAA and average microshoots was 7 shoots/bottle. While inflorescence as explant was best for initiation during dry season on solid medium containing 9 mg/l BAP + 1 mg/l IAA and average microshoots was 18 shoots/bottle. On multiplication stage, sucker produced microshoot on medium containing 4.5 mg/l BAP + 1.0 mg/l kinetin (2.9 shoots). While inflorescense produced microshoot on medium containing 6.0 mg/l IAA + 2.0 mg/l BAP (2.4 shoots). Acclimatization of plantlets produced from sucker and inflorescense was best on medium of compost 75% + soil 25%. Key words: micropropagation, sucker, inflorescense, banana Rajabulu (Musa AAB Group)

Perbanyakan Ruskus (Ruscus hypophyllum L.) secara In Vitro Agus Purwito; Prima Muklisa; Awang Maharijaya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 2 (2005): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

These experiments were aimed to obtain optimum medium for micropropagation of Ruscus. There were two experiments consist of in vitro shoots proliferation, shoot elongation and rooting. The experiment of shoot proliferation performed by inducing adventitious shoots from explant in the Murashige and Skoog (1962)(MS) basal medium supplemented with combination of plant growth regulators BAP (0.0, 0.5, 1.0, 2.0, 4.0 and 6.0 mg/l) and IAA (0.0, 0.1, 0.2 and 0.4 mg/l). The elongation and rooting of plantlets were induced in the different concentration of the MS basal medium (0.5, 1.0 and 2.0 strength) combined with IBA (0.0, 1.0, 2.0, 3.0 and 4.0 mg/l). Both experiments were arranged as completely randomized design with 15 replications. Adventitious shoots were produced in all medium supplemented with BAP with or without IAA. However MS medium supplemented with BAP 1 mg/l or 2 mg/l combined with IAA 0.2 mg/l were the best. The number of adventitious shoots in these medium were 9.2 and 9.4 shoots after 8 weeks cultured respectively. Increasing concentration of BAP more than 4 mg/l decreased number and size of adventitious shoots. The plantlets produced in the proliferation medium were then transferring to the next treatments for elongation and rooting. The best medium for elongation and rooting were medium with half strength of MS with or without IBA. Acclimatization conducted by transferring the rooted plantlets on the medium containing sterilized soil and rice husk charcoal (1:1). After 4 weeks acclimatization, 60-100 percent of plantlets were survived and growth, depend on treatments. Key words: Acclimatization, adventitious shoot, micropropagation, rooting, Ruscus hypophyllum L.

Perakitan Kultivar Kentang Unggul Indonesia secara Cepat dengan Metode Turunan Klonal Biji Tunggal dan Pra - Evaluasi Secara In Vitro G. A. Wattimena; Agus Purwito; H . M. Machmud; , Samanhudi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 29 No. 3 (2001): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

At least ten years needed to abtain new potato cultivar through sexual hybridization, somatic hybridization or through genetic transformation, To short cut this process, Laboratory of Biotechnology, Department of Agronomy, IPB employed a strategy so called single seed in vitro clonal decent ( SSICD) by usings elected parental lines for TPS (True Potato Seed) production. This breeding consist of in vitro pre evaluation for resistance wilt, fusarium wilt, black leg, rot knot nematode and maturity. Using the same number of bacterial cell / (109 cell/ml), there were positive correlation between in vitro test for disease resistance through dripping test or dripping test with greenhouse test through direct inoculation of Ralstonia solanancearum. Resistant clones to fusarium wilt and verticillium were also resistant to bacterial wilt. In vitro tuberization could be use to evaluate maturity of potato cultivar. Key words: Potato, SSICD

ISOLASI DAN REGENERASI PROTOPLAS DARI MESOFIL DAUN KENT ANG (Solanum tuberosum L) DIHAPLOID Agus Purwito; G. A. Wattimena; Antonius Suwanto; Inez H.S. Loeddin Suharsono; Hajrial Aswidinnoor
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 27 No. 1 (1999): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid cultivars of potato BF 15 and SVP 10 leaves used four different enzymes solution. Protoplast were cultured onto four different cultures media to increase plating efficiency. Calli were then transferred to ten different regeneration media. Using cellulose RS 0.5 % and pectolyase Y-23 0.05 % protoplast yield of both cultivars were improved. Medium VKM supplemented with 0.2 mg/l 2, 4-D, 1.0 mg/l NAA and 0.5 mg/l zeatin or 2iP were increase recovery of colonies from protoplast up to 5.9%. Regeneration medium containing zeatin did always produce more shoots than those of 2iP. Genotypes dependant regeneration frequencies have also been showed in this experiments

Uji Ketahanan in Vitro Klon-klon Kentang Hasil Persilangan Kentang Kultivar Atlantic dan Granola terhadap Penyakit Layu Bakteri (Ralstonia solanacearum) dan Busuk Lunak (Erwinia carotovora) Awang Maharijaya; Muhammad Mahmud; Agus Purwito
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 2 (2008): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Several bacterial pathogens can cause diseases of potato. Ralstonia solanacearum and Erwinia carotovora are those of the world’s most important diseases of potato, especially in tropical climates. This experiment was aimed to obtain putative potato cultivars having good tolerance to bacterial wilt caused by R. solanacearum and soft rot caused by E. carotovora. Two adopted potato cultivars in Indonesia, cv. Atlantic (2n=4X=48) and cv. Granola (2n=4X=48), were used as parents. The experiment was arranged in a Randomized Complete Design. Test for tolerance was perfomed in vitro using BF15 as susceptible control and Solanum stenotonum as tolerance control. The result showed that there was high diversity of tolerance level to both R. solanacearum and E. carotovora. Some clones showed good tolerance level as compared to cv. Atlantic and cv. Granola, but none showed that as compared to S. stenotonum.

Co-Authors , Asnawatr , Nurhasanah , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amrullah, Rizki Abi Anas D Susila Andri Ernawati Anjani, Imas Galuh Antonius Suwanto Arif Satria Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atra Romeida Awang Maharijaya Bambang S. Purwoko Bambang Sapta Purwoko Branco, Luis Manuel Budi Marwoto Budi Marwoto Budi Marwoto Budi Winarto Dewi Sukma Dian Puji Rahayu Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih Endah Retno Palupi Endang Gunawan ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fathur Rahmi, Atika Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Harti, Heri Herison, Catur Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISKANDAR ZULKARNAEN SIREGAR ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Juang Gema Kartika Julius D. Nugroho Kalsum Yulifar, Andi Sri Ummi Kartiman, Roni Kartiman, Roni Karyanti ,, Karyanti Kikin H Mutaqin Komang Trisna Wirakusuma Kosasih, Akhmad KRISTIANTO NUGROHO, KRISTIANTO Laela Sari Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Maharijaya, dan Awang Marlin Marlin MASKROMO, ISMAIL Mastur Matjik, Nurhayati Anshori MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi MUHAMMAD HERMAN Muhammad Herman Muhammad Mahmud Muhammad Reza Zakie MUHAMMAD SYUKUR Ni Made Armini Wiendi Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie Nurul Khumaida Nurwita Dewi Prasetyawati , Adinda Prima Muklisa Purba, Dumaris Priskila Putra, Heriansyah Putri, Halida Adistya RAGAPADMI PURNAMANINGSIH Ragapadmi Purnamaningsih Ratna Trisnawati Ravenska, Nidya Rd. Selvy Handayani Rd. Zainal Frihadian REFLINUR REFLINUR Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah Roedhy Poerwanto Roni Kartiman Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati Rr. Sri Hartati RR. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rustikawati S. M. Sumaraow S. M. Sumaraw S. Sudarsono Sari, Dewi Citra Sari, Laela SATRIYAS ILYAS Sembiring, Rinawati SIENTJE MANDANG SUMARAW Sientje Mandang Sumaraw SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Slamet, Alim Setiawan Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono, dan SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO Sudirman Yahya Suhartanto , M. Rahmad Suhartanto, Muhammad Rahmad Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syaiful Anwar Syarifah Iis Aisyah TRI JOKO SANTOSO Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Warid Willy Bayuardi Suwarno Winarto, dan Budi Wulandari, Dyah Retno Wulandari, Dyah Retno Yopi Kurniawan

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