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Suppementation of Kelor Leaf (Moringa oleifera) Aqueous Extract Increase on Post-Thawed Limousin Bull Sperm Quality Wajdi, Syuhuud Arumbinang; Utomo, Budi; Rimayanti, Rimayanti; Safitri, Erma; Suprayogi, Tri Wahyu; Wurlina, Wurlina
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.249-255

Abstract

The purpose of this research was to determine the best dosage of Moringa oleifera Aqueous extract in egg yolk skim milk extender for post thawed Limousin Bull sperm quality on aspect viability, and the level of. The treatment was divided into five groups: egg yolk and skim milk diluter (P0), 2,5% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P1), 5% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P2), 10% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P3), 20% M. oleifera aqueous ekstract in 4 ml egg yolk skim milk (P4). The sperm quality was observed post thawing. The data were analyzed using ANOVA and Duncant Test. The best sperm motility showed on P2 with 43b ± 5,70, the best sperm viability showed on P3 with 58,20b ± 8,72 and than the lowest level of malondialdehyde showed on P4 with 5,434a ± 1,034. In conclusion addition of M. oleifera on dose 10% can increase quality of Limousin Sperm Post Thawed.
Efficacy of Grass and Concentrate Ratio on Milk Urea Nitrogen, Progesterone, and Pregnancy Status in Friesian Holstein Hardaningtyas, Enggar; Mustofa, Imam; Madyawati, Sri Pantja; Rimayanti, Rimayanti; Safitri, Erma; Hermadi, Herry Agoes
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.207-212

Abstract

This study was conducted to know the influence of grass to concentrate ratio to milk urea nitrogen (MUN) level, progesterone level and pregnancy status in Friesian Holstein. A total of 18 dairy cows were randomly selected from the population. The cows were grouped based on feed and MUN and pregnancy. Milk sample collection was carried out once on the seventh day after estrus (D7) and tested with the Barthelot method for measuring urea nitrogen levels. Blood samples were taken three times, on the day of estrus (D0), seven days after estrus (D7), and twenty-two days after estrus (D22), and were tested using Immuno-Sorbent Assay (ELISA). Pregnancy examination was carried out at D90 after artificial insemination through rectal palpation. Based on the analysis, grass to concentrate ratio, progesterone level and MUN level showed no significant difference (p>0.05) but it showed significantly different on milk yield (p<0,05). Based on pregnancy status, progesterone level at D22 showed significantly different whereas at D0 and D7 showed insignificantly different. MUN level and grass to concentrate level also showed significantly different. It can be concluded that the ratio of grass and concentrate did not increase MUN level, progesterone level and pregnancy rate.
Estrogen Concentration on Friesian Holstein Crossbred with Supplementation Feed Cassava Peel Zulfarniansyah, Akhmad Baihaqi; Safitri, Erma
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.94-97

Abstract

The purpose of this study was to determine the ratio of estrogen concentrations given cassava peel feed supplementation. A total of 24 cows of Friesian Holstein, weighing 250-300 kg were divided into two groups and each group contains 12 replications. The first group is Friesian Holstein breeds with the condition that they are given elephant grass feed ad libitum and 25 kg of tofu dregs. The second group is Friesian Holstein breeds with the condition that they are fed ad libitum elephant grass, 25 kg of tofu dregs, and 25 kg of cassava peel. The milk samples were then taken to be tested for estrogen levels using the direct competitive ELISA. Analysis of estrogen concentration data was then carried out by normality test using Shapiro Wilk test then followed by the Paired T-test with a significance level of 5%. Based on the Paired T sample correlation test was 0.072 (p>0.05), which means that the feed gave the cassava peel and not the cassava peel was negatively associated. Based on the Paired T-test is 0.193 (p>0.05), which means there is no significant relationship between the 2 test groups. It can be concluded that there is no significant difference in the estrogen concentration of Friesian Holstein cattle fed with and without cassava peel supplementation.
The Potential of Kaliandra Honey (Calliandra sp.) on Sertoli Cell Counts in Malnourished Albino Rats Erlangga, Made Bagus; Srianto, Pudji; Safitri, Erma; Rimayanti, Rimayanti; Hernawati, Tatik; Hermadi, Herry Agoes
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.88-92

Abstract

This study was performed to investigate the effect of calliandra honey (Calliandra sp.) on the number of sertoli cells in malnourished male albino rats. A total of 24 male rats were divided into four groups, i.e. (K) without treatment, (P0) fasted, (P1 and P2) respectively fasted and administered honey concentrations of 30% and 50% for 10 days after fasting. The results showed that administration calliandra honey with a concentration of 30% and 50% significantly increased the number of sertoli cells. It can be concluded that administration calliandra honey can have a positive effect on malnourished male rats, especially in improving the number of sertoli cells.
Evaluation of Motility, Viability, and Integrity Plasma Membranes of Frozen Semen in Friesian Holstein with Storage Periods of 33, 30, 27, and 24 Years Purnawan, Ahmad Budi; Rimayanti, Rimayanti; Susilowati, Suherni; Mustofa, Imam; Hernawati, Tatik; Safitri, Erma
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.162-172

Abstract

The quality of frozen semen is one of the components of the artificial insemination program. The freezing method, kind of diluent, handling of frozen semen, and thawing process all affect the quality of frozen semen. This study aimed to determine the motility, viability, and plasma membrane integrity of frozen semen of Friesian Holstein cattle at storage periods of 33, 30, 27, and 24 years. The samples used were stored for 33 years (production 1987), 30 years (production 1990), 27 (1993 production), and 24 years (1996 production) stored in Singosari Artificial Insemination Center, Malang. Data were statistically analyzed using One-Way ANOVA followed by Duncan's Multiple Range Test (p < 0,05). Based on the study's findings, it can be concluded that the motility, viability, and plasma membrane integrity of frozen semen that had been preserved for 33, 30, 27, and 24 years were still acceptable and in good condition in comparison to the SNI quality criteria for bull frozen semen in Indonesia.
Protective Effects of Apis Dorsata Honey Supplementation on Kidney Histopathology in Mice with Monosodium Glutamate Exposure Rao, Sherbrina Bai Seenivasa; Widjiati, Widjiati; Lokapirnasari, Widya Paramita; Triakoso, Nusdianto; Safitri, Erma; Kuncorojakti, Suryo; Proboningrat, Annise
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.63-69

Abstract

This study was conducted to investigate the protective effects of Apis dorsata honey on the histopathological changes of the kidney in mice exposed to monosodium glutamate. This study used 25 male mice as the experimental animal which were divided into five groups with five replication, respectively i.e. (C-) was administered basal diet, (C+) was administered 4 mg/g BW MSG, (T1) was administered A. dorsata honey of 53.82 mg/20g BW followed by MSG 4 mg/g BW, (T2) was administered A. dorsata honey of 107.64 mg/20g BW followed by MSG 4 mg/g BW, and (T3) was administered A. dorsata honey of 161.46 g/20g BW followed by MSG 4 mg/g BW. All treatments were carried out orally for 52 days. This study was observed tubular epithelial hydropic degeneration, epithelial necrosis, and glomerular necrosis. Data were analyzed using Kruskal-Wallis test followed by the Mann-Whitney test. As a result, for the epithelial and glomerular necrosis variables, the T3 group showed significant results when compared with C+, T1, and T2 groups. Moreover, T3 was not significantly different from the C- group. It can be concluded that A. dorsata honey in T3 group could protect mice kidneys from the damaging effect of MSG.
PENGARUH PEMBERIAN PROPOLIS LEBAH TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT (Mus musculus) BETINA YANG DIPAPAR LOGAM BERAT PB ASETAT [Pb(C2H3O2)2] Rachmani, Sartika Dewi; Safitri, Erma; Darsono, Roesno; Hestianah, Eka Pramyrtha; Plumeriastuti, Hani; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19988

Abstract

Lead acetate in the hepatobiliary system may cause peroxidation catalysis of unsaturated fatty acids, reduce nitrogenoxide and increase hydroxyl radical. Lead acetate produces oxidative stress characterized by free radical formation and inhibits lipid peroxidation. Giving antioxidants can neutralize free radicals from the detrimental effects that arise on the process or excess oxidation reactions. The purpose of this research was to find out how the effect of bee propolis on histopathologic images of hepatic mice (Mus musculus) of females exposed to lead acetate [Pb(C2H3O2)2]. The subjects were 25 mice (Mus musculus) mushulus of BALB/C strain with average weight 25-30 gram and 8 weeks old, divided into 5 treatment groups, each consisting of 5 heads per group. The K- group was given a Tween 80 solution at a doses of 0.5 mg/kgBW for 20 consecutive days. The K+ group, which was given only lead acetate at a doses of 10 mg/kgBW orally for 10 days. P1, P2, and P3 were given 10 mg/kgBW lead acetate solution orally for 10 days. The following 10 days were given bee propolis with doses of P1 200 mg/kgBW, P2 400 mg/kgBW, and P3 800 mg/kgBW. On the 21th days the mice were dissected, to observe the extent of the damage. All data were performed using a statistical test with Kruskal Wallis test and if there was a marked difference between treatment groups (p<0.05), then the Mann-Whitney test was followed. The results obtained that bee propolis can repair hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate. Increased dose of bee propolis is ineffective in repairing hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate.
Ovariohysterectomy in Cat as An Alternative Handling of Fetus Mummification Cases Ercha Aulia Putri, Cindy; Safitri, Erma; Syafitri, Winda
Media Kedokteran Hewan Vol. 36 No. 1 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i1.2025.50-67

Abstract

Fetal mummification is a rare and challenging condition in cats that requires immediate intervention to mitigate potential risks to the mother and future pregnancies. This case study presents the diagnosis, treatment, and outcomes of Pawpaw, a cat diagnosed with fetal mummification. Ovariohysterectomy was performed as an alternative treatment method to address the critical condition of the cat. The diagnosis was established based on various factors, including indications, anamnesis, clinical symptoms, clinical examination, clinical findings, and investigative results. Diagnostic confirmation was achieved through blood haematology tests and ultrasound examinations. The therapeutic approach consisted of administering a combination of normal saline fluid therapy, glycerin, ondansetron, ceftriaxone, intramox, doxycycline, acetylcysteine, sakatonic liver, vitamins, ventolin, Tripura, meloxicam, impacting, and furosemide. Anesthesia during the ovariohysterectomy procedure involved the use of atropine sulfate, xylazine, and ketamine. Despite the extensive medical intervention, the cat, Bimbim, succumbed to its critical condition during the surgery. This case study highlights the importance of early diagnosis and prompt intervention in cases of feline fetal mummification. Ovariohysterectomy proved to be a viable alternative treatment option, although the critical condition of the cat posed significant challenges to its successful outcome. Further research and enhanced understanding of fetal mummification in cats are essential to improve the management and prognosis of this condition.
The Correlation between the Weight of Ovary, Number of Follicles, and Quality of Oocytes of Culling Female Bovine in Slaughterhouse Chandramohan, Koshini; Utama, Suzanita; Lestari, Tita Damayanti; Rimayanti; Safitri, Erma; Hestianah, Eka Pramyrtha; Mulyati, Sri; Damayanti, Ratna; Khairullah, Aswin Rafif; Hasib, Abdullah
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.206-220

Abstract

The purpose of this research was to determine the correlation between the weight of the ovary, the number of follicles, and the quality of the oocytes from the culling female bovine   obtained from the slaughterhouse. The variation may affect the potential of the ovary to produce follicles. Paired ovaries were obtained from female bovines.  Follicle numbers were recorded and divided into three size categories (small: 3 mm, medium: < 3 mm-8 mm, and large: > 8 mm). Oocytes were aspirated and the number of oocytes was recorded and graded into four categories (grades A, B, C, and D). There was a positive correlation between the weight of the ovary and the number of follicles, which is 0.560 with the regression equation y = 3.52 + 0.501 x. There was a positive correlation between the number of follicles and the number of oocytes, which is 0.546 with the regression equation y =2.48 + 1.204 x. There was a positive correlation between the number of oocytes and the grade of oocytes, which is 0.520, with the regression equation y = 0.93 + 0.800 x. There was no correlation between the weight of the ovary and oocyte grade A, which is 0.013.
Probiotic Lactobacillus sp. as a Substitute for Growth Antibiotic (AGP): Its Effects on Production Performance and Contribution Margin Value in Broilers Infected with Escherichia coli Firdaus, Muhammad Aviv; Soeharsono, Soeharsono; Lokapirnasari, Widya Paramita; Hidanah, Sri; Kusnoto, Kusnoto; Safitri, Erma; Silfia, Himatul Ilma; Lisnanti, Ertika Fitri
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 3 (2024): December 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.03.2

Abstract

This study aimed to show that the probiotic Lactobacillus sp. could increase production performance (feed consumption, feed conversion, feed efficiency, body weight gain and body weight) and the contribution margin value in broiler chickens infected with Escherichia coli. This experimental study utilized a completely randomized design and involved 40 male broilers. The broilers were infected with Escherichia coli at the age of 21 days, and the study lasted for 35 days. The study consisted of 4 treatment groups, with 10 chickens in each group. The treatment groups were as follows: P0 (negative control; no feed additive; no E. coli infection), P1 (positive control; no feed additive; E. coli infection), P2 (AGP (zinc bacitracin) added at 1 g/kg mixed through commercial feed; no probiotic Lactobacillus sp.; E. coli infection), and P3 (commercial feed no AGP; probiotic Lactobacillus sp. added at 5 mL/L through drinking water; E. coli infection). The data were analysed via a general linear model (repeated measures) test of ANOVA followed by Duncan's multiple distance test. The results revealed significant differences (p<0,05) in production performance, including feed efficiency and final body weight. The highest contribution margin value shown by the P3 treatment group (administering probiotic Lactobacillus sp. in drinking water) was IDR 81,570, and the lowest value shown by the P2 treatment group (administering AGP to feed) was IDR 35,468. In conclusion, the addition of the probiotic Lactobacillus sp. at a concentration of 5 mL/L through drinking water improved production performance and increased the contribution margin value in broilers infected with E. coli.
Co-Authors Abdullah Abdullah Agustono, Bodhi Al arif, Mohammad Anam Alfanindya, Evita Aisyah Alfina Azkiana Amrullah, Muhammad Fajar Annise Proboningrat Boedi Setiawan Budi Utomo Chandramohan, Koshini Damayanti L, Tita Damayanti, Tita Darsono, Roesno Dikman, Dicky Mohammad Djoko Legowo Eha Renwi Astuti Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Ercha Aulia Putri, Cindy Erlangga, Made Bagus Ernie Maduratna Setiawati Febrianila, Rosiana Firdaus, Muhammad Aviv Haditanojo, Wiyanto Hana Eliyani Hani Plumeriastuti Hardaningtyas, Enggar Hardijanto, Hardijanto Hartono Hartono Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Husni Anwar Iis Aprinawati, Iis Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Imam R., Tjuk Inggriani, Fauzia Irfani, Dyah Irvanto, Rudi Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Kuncorojakti, Suryo Kurniasari, Pitaloka Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Kusuma, Agung Jati Lisnanti, Ertika Fitri Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mafruchati, Maslichah Mayola, Dilla Aranda Meydilasari, Nanda Irsa Mirni Lamid Mufasirin Nawangwulan, Tantri Nunuk Dyah Retno Lastuti Nusdianto Triakoso Paramita L., Widya Pasang, Gabriel Sampe Praja, Ratih Novita Pramudita, Kautsar Pandu Prastika, Zeni Prastiya, Ragil Angga Prastyorini, Vina Ari Pratomo, Andrika Indra Prayogo, Danu Pudji Srianto Purnawan, Ahmad Budi Putri, Natalie Lovena Pramono Rachmani, Sartika Dewi Raharjo, Dadik Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmawati, Kadek Rahmi Sugihartuti, Rahmi Rao, Sherbrina Bai Seenivasa Ratna Damayanti Ratnaningtyas, Nurrohmah Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Sabdoningrum, Emy Koestanti Salahuddin, Muhammad Soleh Santoso, Kuncoro Puguh Saputro, Amung Logam Seagames Waluyo, Seagames Shabira, Zahra Silfia, Himatul Ilma Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati, Sri Pantja Sri Wigati Mardi Mulyani Suherni Susilowati Supranianondo, Koesnoto Supriyadi Supriyadi Susilowati, Suheni Suwarno Suwarno Suzanita Utama Swari, Wahyu Retno Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Trisnawati, Sharfina Habibah Tyasningsih, Wiwiek Umama, Ayu Riza Utama, Dirga Januar Surya Viora, Dwi Viski Fitri Hendrawan Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widya Paramita Widya Paramita Lokapirnasari Winda Syafitri, Winda Wurlina, W Yasmidas, Yasmidas Yudhana, Aditya Yunita, Maya Nurwartanti Yunus, Muchammad Zulfarniansyah, Akhmad Baihaqi