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PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP FOLIKULOGENESIS MENCIT (Mus musculus) Abdullah, Abdullah; Restiadi, Tjuk Imam; Lastuti, Nunuk Dyah Retno; Damayanti, Tita; wurlina, wurlina; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.711 KB) | DOI: 10.20473/ovz.v7i2.2018.102-108

Abstract

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subject of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatment were K0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are number of primary, secondary, tertiary and de Graff follicles. During the treatment the estrus cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affect (p<0,05) on increasing of the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicle significantly (p<0,05).
EFISIENSI REPRODUKSI SAPI PERANAKAN LIMOUSIN AKSEPTOR INSEMINASI BUATAN DI KECAMATAN TIKUNG, KABUPATEN LAMONGAN TAHUN 2016 Prastyorini, Vina Ari; Supranianondo, Koesnoto; Safitri, Erma; Restiadi, Tjuk Imam; wurlina, wurlina; Hernawati, Tatik
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.747 KB) | DOI: 10.20473/ovz.v7i2.2018.160-163

Abstract

The purpose of this study was known the difference of reproductive performans Limousine Cross Breed that haved artificial insemination from diffrence straw from BBIB Singosari and BBIB Lembang covering Conception Rate, Service per Conception, and Calving Rate in period 2016th. This study uses survey research which data from primary and secondary. Primary data were obtained from interview with Limousine Cross Breed breeders and secondary data were obtained from artificial insemination officer's record. The observed variables are Conception rate (CR),  Service per conception (S/C) and Calving rate  (CvR). Data were analyzed descriptive that average and Chi Square.Result of research showed no significant difference (p>0.05) on CR, S/C and CvR between Limousin cross breed cows that haved artificial insemination from BBIB Singosari and BIB Lembang.
EFEK PEMBERIAN L-ARGININ TERHADAP GAMBARAN HISTOLOGI JUMLAH SPERMATOSIT PRIMER PADA MENCIT (Mus musculus) SETELAH TERPAPAR SUHU PANAS Utama, Dirga Januar Surya; Susilowati, Suherni; Hernawati, Tatik; safitri, Erma; Mulyati, Sri; Nurhajati, Tri
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.798 KB) | DOI: 10.20473/ovz.v8i1.2019.22-25

Abstract

The purpose of this study was to know the effect of L-arginine on the histology of primary spermatocyte count in mice (Mus musculus) after high temperature exposed. The subjects of this study were 20 adult male mice, 8 weeks old with an body weight range from 20-40 grams. This research conducted by using Complete Randomized Design (RAL) with 4 treatments and 5 replications. The treatments consisted of, P0- = treatment with 1ml of aquabidest without high temperature exposed, P0 + = treatment with 1ml of aquabidest after high temperature exposed for 1hour per-day for 35 days, P1 = 1.3mg / day L-arginine dissolved in 1ml aquabidest and given orally after high temperature exposed for 1hour per-day for 35 days and P2 = 2.6mg / day L-arginine dissolved in 1ml aquabidest given orally after high temperature exposed for 1hour per-day for 35 days. Observations done by making histologic preparations of testicular organs and then calculated the total number of primary spermatocyte per treatment. The data of primary spermatocyte calculated and analyzed by using Analysis of Variance (ANOVA), followed by Duncan test. The result from data analysis showed that there was a significant difference (p<0.05) between P1 and P2 with control,between P1 and P2 the analysis did not show any significant  difference (p> 0.05).
KORELASI ANTARA LINGKAR DADA, PANJANG BADAN DAN TINGGI GUMBA TERHADAP VOLUME SEMEN DAN KONSENTRASI SPERMATOZOA SAPI MADURA Mayola, Dilla Aranda; Rimayanti, Rimayanti; Warsito, Sunaryo Hadi; Utama, Suzanita; Eliyani, Hana; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.57 KB) | DOI: 10.20473/ovz.v8i2.2019.105-109

Abstract

The aim of this research was to know the correlation between heart girth, body length, and wither's height with semen volume and sperm concentration of Madura Bulls. Ten Madura bulls aged three to four years from Madura  were measured. Variables observed were heart girth, body length, wither's height, semen volume and sperm concentration. Data were analyzed by multiple linear regression analysis. The result of this research showed that there was no correlation between heart girth, body length, and wither's height with semen volume Y = 25,212 + 0,190 X1+ (-0,131) X2 + 0,136 X3, so as there was no correlation between heart girth, body length and wither's height with sperm concentration Y = 8739,559 + (-36,663) X1+ (19,714) X2 + 6,2960 X3. And than was no correlation between semen volume and sperm concentration Y = 7,303 + (-0,002) X1.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM KUNING TELUR TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Swari, Wahyu Retno; Sabdoningrum, Emy Koestanti; Wurlina, Wurlina; Susilowati, Suherni; Kurnijasanti, Rochmah; Safitri, Erma
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.407 KB) | DOI: 10.20473/ovz.v8i2.2019.122-126

Abstract

The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,5% green tea extract in skim milk and egg yolk diluent, 0,1% green tea extract in skim milk and egg yolk diluent, and 0,15% green tea extract in skim milk and egg yolk diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after being diluted. The data obtained was analyzed with the Analysis of Variant (ANOVA), followed by multiple range test duncan. The results showed that the highest percentage of motility, viability and intact plasma membrane derived from green tea extract 0,15% on skim milk and egg yolk diluent  for 1 day storage is 86.66b ± 2.58, 92.00b ± 1.78, and 74.16b ± 3.25. The lowest percentage of motility, viability and intact plasma membranes was obtained from the skim milk and egg yolk diluent for 5 days storage is 43.33a ± 6.05, 56.50a ± 3.08 and 28.33a ± 2.80. The conclusion of this study is the addition of 0.15% green tea extract in a skim milk and egg yolk diluent can maintain the quality of sperm for up to 5 days of storage at cold temperatures.
Hubungan antara skor hasil bacaan heat detector dengan gambaran sitologi vagina sebagai parameter penentuan estrus pada kambing Meydilasari, Nanda Irsa; Hernawati, Tatik; Lestari, Tita Damayanti; Safitri, Erma; Sardjito, Trilas; Ismudiono, Ismudiono; Hidanah, Sri
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.361 KB) | DOI: 10.20473/ovz.v9i1.2020.7-11

Abstract

This study aimed to determine the relationship between heat detector reading results and the feature of vaginal swab preparations as a parameter for determining estrus in Peranakan Etawa goats. Five healthy non-pregnant goats weighing 20-30 kg, were synchronized using PGF2α (7.5 mg Dinoprost, Enzaprost) intramuscularly twice with 11 days interval. Vaginal cytology examination began the day after the second PGF2α injection. The heat detector (Draminski®, DramiÅ„ski SA, Poland) is an estrus detecting device based on the amount of electric current flowing through the vaginal mucus. Spearman correlation analysis showed a relationship (p <0.05) between heat detector reading and estrus detection based on vaginal cytology. The heat detector readings were very significantly correlated positively (p <0.01) with the number of epithelial cells (coefficient correlation, r= 0.564), but negatively correlated with the number of cornified cells (r = –0.854), and also significantly correlated positively (p <0.05) to the number of leukocytes (r = 0.487). The estrus stages were significantly correlated positively (p <0.05) only to the number of leukocytes (r = 0.918). Likewise, the number of epithelial cells was highly correlated negatively (p <0.01) only to cornified cells (r = –0,643), and the number of cornified cells was significantly correlated negatively (p <0.05) only to the number of leukocytes (r = –0.472). It could be concluded that the features of the proestrus, estrus and metestrus stages based on the goat vaginal cytology was correlated with the reading results of the heat detector.
Pengaruh injeksi nikotin terhadap motilitas, viabilitas, dan integritas membran spermatozoa mencit (Mus Musculus) Pramudita, Kautsar Pandu; Lokapirnasari, Widya Paramita; Susilowati, Suheni; Ratnani, Hermin; Safitri, Erma; Luqman, Epy Muhammad
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.77-81

Abstract

The process of spermatogenesis is sensitive to nicotine, a main component of cigarette smoke. This study aimed to observe the effect of nicotine injection on motility, viability, and membrane integrity of the spermatozoa of mice (Mus musculus). The mice were 10 weeks old, weighing about 20 grams, and in healthy and fertile conditions. Mice were adapted for 1 week by being given feed in the form of pellets and drinking water every day. Twenty of the mice were divided into four groups. Group K as a control was injected with 0.9% NaCl at 2.5 mg/Kg bw. Group P1 was injected with nicotine at 2.5 mg/Kg bw. P2 was injected with nicotine at 5 mg/Kg bw. Group P3 was injected with nicotine at 10 mg/Kg bw. The injection was carried out subcutaneously as much as 0.5 ml once a day for 35 days. Data analysis used the Analysis of Variances (ANOVA), followed by Duncan's Multiple Range Test. The results showed that the viability, motility, and membrane integrity of the spermatozoa decreased significantly (p <0.05) according to the increase in the dose of nicotine injected. It could be concluded that subcutaneous nicotine injection linearly decreased the motility, viability, and membrane integrity of the spermatozoa of mice.
TRAINING ON ANIMAL FEED PROCESSING AND TREATMENT OF REPRODUCTIVE DISORDERS IN PALM INTEGRATED CATTLE FARMING AREA IN WEST KALIMANTAN Hidayatik, Nanik; Lamid, Mirni; Hermadi, Herry Agoes; Warsito, Sunaryo Hadi; Suprayogi, Tri Wahyu; Safitri, Erma; Pratomo, Andrika Indra
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 8 No. 1 (2024): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v8i1.2024.114-120

Abstract

West Kalimantan Province is one of the provinces that has a large area of oil palm plantations which can become integrated cattle farms. Oil palm plantations provide a source of feed that comes from palm oil processing waste and green grass around the oil palm trees. However, this waste contained not sufficient nutrients if given directly, so needed feed processing to increase the nutrition. The community service team from the Faculty of Veterinary Medicine, Universitas Airlangga carried out activities to increase the knowledge and skills of farmers in processing palm oil waste feed and carried out reproductive examination activities on female cattle. The output target to be achieved is increasing the ability of farmers to make feed from palm oil waste that can sufficient for livestock nutritional needs and increased of reproductive health of cows. This activity held on 20 – 23 July 2023 in Tonang Village, District. Sengah Temila, Kab. Landak, West Kalimantan. The activity was attended by 40 participants consisting of breeders, medical officers and veterinarians from the Dinas Ketahanan Pangan, Pertanian dan Perikanan Kabupaten Landak dan Dinas Perkebunan dan Peternakan Provinsi Kalimantan Barat. The first day's activities were carried out by delivering outreach and training materials on processing palm oil waste feed through silage and fermentation methods, making UMB and UMMB, and carrying out reproductive examinations and treatment of female cattle. The outcomes that have been achieved include increasing farmers' understanding and skills regarding how to make silage and palm frond fermentation, as well as UMB and UMMB as additional feed. Health and reproductive disorders examination of female cattle were carried out on 2 female cattle belonging to the Koperasi Produsen Landak Mandiri and 17 female cattle belonging to the Alam Raya Hijau Group. Based on the examination, data was obtained that 7 cows were pregnant, 1 cow had endometritis, and 11 cows were declared to be in normal reproductive condition. The conclusion that can be drawn from this activity is that the participants' knowledge about the given topic increases and the reproductive health of female cattle can be handled with appropriate examination and treatment.
Evaluasi Penyuluhan Penyakit Infeksius dan Reproduksi Ternak di Blitar Raya Suwarno, Suwarno; Safitri, Erma; Rahmahani, Jola; Rahmawati, Kadek; Supriyadi, Supriyadi; Pasang, Gabriel Sampe; Irfani, Dyah; Kurniasari, Pitaloka
Bima Abdi: Jurnal Pengabdian Masyarakat Vol. 5 No. 2 (2025): Bima Abdi: Jurnal Pengabdian Masyarakat
Publisher : Yayasan Pendidikan Bima Berilmu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53299/bajpm.v5i2.1578

Abstract

Blitar Raya (Kota dan Kabupaten) merupakan salah satu kantong ternak di Jawa Timur. Merebaknya kasus PMK (Penyakit Mulut dan Kuku) dan LSD (Lumpy Skin Disease) menyebabkan turunnya populasi ternak. Kasus yang dihadapi pemerintah setempat masih adanya kasus PMK dan LSD meskipun vaksinasi dan biosekuriti sudah dilakukan. Dampak lain akibat kedua jenis penyakit tersebut adalah menurunnya produksi susu dan gangguan reproduksi. Pengabdian kepada Masyarakat ini dilakukan untuk memberikan penyuluhan terkait materi tentang Virologi (program vaksinasi dan biosekuriti) dan Reproduksi ternak (deteksi birahi dan repeat breeding). Peserta kegiatan ini adalah para Dokter Hewan dan Paramedis yang ada di wilayah Blitar Raya. Ceramah dan diskusi dilakukan dengan mengundang para peserta untuk mengikuti kegiatan penyuluhan di ruang pertemuan RM Bu Mamik Kota Blitar. Materi disajikan dalam bentuk power point menggunakan LCD. Di akhir diskusi peserta diberikaan soal dalam bentuk kuesioner, dengan topik materi tentang Virologi dan Reproduksi. Topik materi Virologi dibagi lagi menjadi subtopik vaksinasi dan biosekuriti, sedangkan topik materi Reproduksi dibagi menjadi subtopik deteksi birahi dan repeat breeding. Penilaian kuesiononer didasarkan atas jawaban benar-salah dan kemampuan peserta di dalam menganalisis topik dan subtopik yang diberikan. Penilaian didasarkan atas pengetahuan peserta beda strata pendidikan terhadap materi yang diujikan. Kesimpulan dari hasil penilaian adalah strata pendidikan dan pengalaman lapangan memiliki peran penting di dalam menguasai topik materi penyuluhan.
Erythrocyte, Hemoglobin, and Hematocrit Profiles in Indonesian Simental and Limousin in Highlands and Lowlands in Banyuwangi Trisnawati, Sharfina Habibah; Safitri, Erma; Agustono, Bodhi; Luqman, Epy Muhammad; Saputro, Amung Logam; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.78209

Abstract

This study aimed to evaluate the differences in blood profile of erythrocytes, hemoglobin, and PCV in Indonesian simental and limousin beef cattle in highlands and lowlands. Banyuwangi is one of the beef cattle contributors in East Java with its highland and lowland topography. This research was conducted from July to August 2023. The research was conducted in 3 locations namely highlands (Kalibaru), lowlands (Rogojampi) and sample examination in Airlangga University Banyuwangi. Animals used in this study were Indonesian simental and limousin beef cattle with 1-2 years of age as many as 15 heads in the highlands and 15 heads in the lowlands. The research method used was purposive sampling. Blood samples used in this study used Indonesian simental and limousin beef cattle blood taken using venoject and inserted in EDTA tubes and examined using a Hematology Analyzer. The variables observed in this study were the number of erythrocytes, hemoglobin, and PCV. The data obtained were processed with Independent T-test data analysis. The results showed no significant difference in erythrocytes and PCV, but there was a significant difference in hemoglobin levels. The conclusion of this study is that Indonesian simental and limousin beef cattle in the lowlands experience mild anemia.
Co-Authors Abdullah Abdullah Agustono, Bodhi Al arif, Mohammad Anam Alfanindya, Evita Aisyah Alfina Azkiana Amrullah, Muhammad Fajar Annise Proboningrat Boedi Setiawan Budi Utomo Chandramohan, Koshini Damayanti L, Tita Damayanti, Tita Darsono, Roesno Dikman, Dicky Mohammad Djoko Legowo Eha Renwi Astuti Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Ercha Aulia Putri, Cindy Erlangga, Made Bagus Ernie Maduratna Setiawati Febrianila, Rosiana Firdaus, Muhammad Aviv Haditanojo, Wiyanto Hana Eliyani Hani Plumeriastuti Hardaningtyas, Enggar Hardijanto, Hardijanto Hartono Hartono Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Husni Anwar Iis Aprinawati, Iis Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Imam R., Tjuk Inggriani, Fauzia Irfani, Dyah Irvanto, Rudi Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Kuncorojakti, Suryo Kurniasari, Pitaloka Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Kusuma, Agung Jati Lisnanti, Ertika Fitri Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mafruchati, Maslichah Mayola, Dilla Aranda Meydilasari, Nanda Irsa Mirni Lamid Mufasirin Nawangwulan, Tantri Nunuk Dyah Retno Lastuti Nusdianto Triakoso Paramita L., Widya Pasang, Gabriel Sampe Praja, Ratih Novita Pramudita, Kautsar Pandu Prastika, Zeni Prastiya, Ragil Angga Prastyorini, Vina Ari Pratomo, Andrika Indra Prayogo, Danu Pudji Srianto Purnawan, Ahmad Budi Putri, Natalie Lovena Pramono Rachmani, Sartika Dewi Raharjo, Dadik Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmawati, Kadek Rahmi Sugihartuti, Rahmi Rao, Sherbrina Bai Seenivasa Ratna Damayanti Ratnaningtyas, Nurrohmah Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Sabdoningrum, Emy Koestanti Salahuddin, Muhammad Soleh Santoso, Kuncoro Puguh Saputro, Amung Logam Seagames Waluyo, Seagames Shabira, Zahra Silfia, Himatul Ilma Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati, Sri Pantja Sri Wigati Mardi Mulyani Suherni Susilowati Supranianondo, Koesnoto Supriyadi Supriyadi Susilowati, Suheni Suwarno Suwarno Suzanita Utama Swari, Wahyu Retno Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Trisnawati, Sharfina Habibah Tyasningsih, Wiwiek Umama, Ayu Riza Utama, Dirga Januar Surya Viora, Dwi Viski Fitri Hendrawan Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widya Paramita Widya Paramita Lokapirnasari Winda Syafitri, Winda Wurlina, W Yasmidas, Yasmidas Yudhana, Aditya Yunita, Maya Nurwartanti Yunus, Muchammad Zulfarniansyah, Akhmad Baihaqi