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Journal : Jurnal Veteriner

Terapi Fotodinamik Mempercepat Kesembuhan Luka Insisi pada Kulit Tikus Putih (Rattus novergicus) yang Diinfeksi Bakteri Methicilin-Resistant Staphylococcus aureus Yonatan Dimascahyo Budianto; Lucia Tri Suwanti; Wiwik Misaco Yuniarti; Hani Plumeriastuti; Wiwiek Tyasningsih; Boedi Setiawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this research was to determine the effect of photodynamic therapy (PDT) on the healing of incision wounds in rat skin (Rattus norvegicus) that infected with MRSA bacteria by evaluating and give a score (0 – 4) at wound healing factors such as epithelialization, angiogenesis, inflammatory cells, fibroblast cells and collagen density. This study used 25 white rats divided randomly into 5 groups with 5 replications, namely P0 = MRSA infection wound + Povidone Iodine, P1 = MRSA infection wound + Silver Sulfadiazine, P2 = MRSA infection wound + 5-minute PDT irradiation, P3 = MRSA infection wound + 10- minute PDT irradiation and P4 = MRSA infection wound + 15-minute PDT irradiation. Histopathological observations and scoring showed epithelialization and collagen density in groups P2, P3 and P4 increased significantly compared to groups P0 and P1 (p<0.05). The histopathological observations and scoring of angiogenesis, inflammatory cells, fibroblast cells of P0 and P1 groups had a higher score than the treatment group with photodynamic therapy P2, P3 and P4 (p<0.05). This condition showed that in the P0 and P1 groups are still in the inflammatory phase, while in the P2, P3 and P4 groups have entered the remodeling phase which is characterized by high epithelialization scores and collagen density. Giving photodynamic therapy to wounds infected with MRSA can accelerate the wound healing process.
Perbandingan Kadar Elektrolit Serum Pascaenterektomi Ekstensif 75 % pada Anjing yang Diterapi dengan Laktoferin Boedi Setiawan; Sudarminto -; Hartiningsih -
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A severe and extensive intestine damages can occur in dogs suffering from volvulus , strangulation,intersuception and neoplasia. Extensive enterectomy is the most common medical treatment for dogssuffering from such disorder. A study was therefore conducted to compare the serum electrolyte level ofextensively enterectomyzed dogs after being treated with different level (0%, 0.05% and 0.5 mg/bodyweight) of lactoferin. As many as 9 dogs at 3-4 moth-old and with the body weights ranging from 4-5 kgwere used. After being enterectomized to the extent of 75% out of the total length of the intestine, the dogswere divided randomly into 3 groups (I, II and III) each of which consisted of 3 dogs. The three groups weretreated for 30 days respectively with 0.0 mg/body weight, 0.05mg/kg body weigth and 0.5mg/kg bodyweight of lactoferin. The electrolyte levels (Na, K and Cl ion) of dogs were determined at days 1, 15 and 30of during the treatment. The data collected from this study were analysed by Analysys of Variance (Anova)proceded by Duncan Multiple Range test (DMRT). The result showed that at days 15 and 30 of thetreatment, the electrolyte levels of the dogs with lactoferin (0.05 and 0.5 mg/body weight) were significantlyhigher than in dogs without lactoferin (0mg/bodyweight). The level of Na ion of dogs with 0,5, 0,05, dan 0mg/kg bw lactoferin were 143, 143,4, dan 141,7 mEq/L respectively at day 15 and 147, 150, dan 137,7mEq/L respectively at day 30. The levels of K ion for those dogs were 5,17, 4,97, dan 3,83 mEq/Lrespectively at day 15 and 30: 5,1, 5,13, 3,73 mEq/L respectively at day 30. Meanwhile, their Cl levelswere 113,7, 114,3, 104 mEq/L respectively at day 15 and 115,3, 117,3, dan 91,3 mEq/L respectively atday 30. Hasil penelitian ini menunjukkan bahwa memperian lactoferin memperbaiki kadar elektrolitserum anjing yang dienterektomi secara ekstensif. It was evidence that lactoferin treatment can improvedthe electrolyte profiles of extensively enterotomized dogs.
Co-Authors A'la, Rofiqul Abdul Samik, Abdul Agus Wijaya Agustono, Bodhi Ahmad Herdianto Wicaksono Aksono HP., Eduardus Bimo Aldin Akbar Rahmatullah Alfina Azkiana Ana Rohmatul Laili Aprinda Ratna Lovela Arimbi Arum Puspitasari Aswin, Ahmad Azami D Azinar Azizah Bilqis Nurkarimah Bambang Poernomo Bambang Sektiari Lukiswanto Budi I Santoso Budi Santoso Budi Utomo Dadik Rahardjo, Dadik Daniel Leonardo Darmawan, Moch. Rizky Dellavia, Yolanda Diyantoro Diyantoro, Diyantoro Djoko Legowo Djoko Legowo Doohan Mahendra Edward Yonas Kristijanto Eighty M Kurniawati Eka Pramyrtha Hestianah, Eka Pramyrtha Eka Pramyrtha Hestinah Emmanuel Djoko Poetranto Endang Suprihati Endang Suprihati Epy Muhammad Luqman Erma Safitri Faisal Fikri Faizah, Ayu Nur Febriyatin, Fierdausi Nur’Aini Fedik A Rantam Fedik Abdul Rantam Fianti Nufinda Rachma Gatut Hardianto, Gatut Ghifari Lutfi Fauzi Hani Plumeriastuti Hari Paraton Hartiningsih - Helen Susilowati, Helen Hendarti, Gracia Angelina Heni Puspitasari Hidajati , Nove Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Indasari, Elly Nur Indriyanti, Astrid Ira Sari Yudaniayanti Ismudiono Ismudiono Ismudiono, Ismudiono Istiana, Izzatul Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Kamilah Nurizza Kartika Aditiya Amelia Khairunnisa, Anindita Koesdarto , Setiawan Kuncorojakti, Suryo Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lastuti , Nunuk Dyah Retno Legowo, Djoko Lestari, Dewi Sri Lilik Maslachah LINA SUSANTI Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Melda Mufidatul Ifqiyyah Mochamad Lazuardi Mohamad Safri Sauqi Mufasirin Mustofa Helmi Effendi Nidom, Chairul Anwar Niken Hastuti, Apriliwiyani Nove Hidajati Nur AR Widiatmoko Nusdianto Triakoso Pinayungan, Probo Probo Warih Tatag Poedji Hastutiek Praja, Ratih Novita Prastika, Dinda Dwi Prastiya, Ragil Angga Pratama, Ponasari Galuh Primarizky, Hardany Pudji Srianto Puput Ade Wahyuningtyas Purbowati, Tri Endah Rachmanita, Rona Taufiqul Rahaju Ernawati Rahmahani, Jola Rahmandari, Dina Agylia Ramadhanty, Miladhiyah Nabila Ratna Damayanti Ratna Damayanti Retno Wulansari Rochmah Kurnijasanti Saputro, Amung Logam Sari, Fifin Kurnia Sesa Puput Febriyanti Setiawan Koesdarto Soeharsono Soeharsono Soeharsono Soeharsono Sofyan, Miyayu Soneta Sri Hidanah Sri Mulyati Srimaryanto, Leonardo Reza Sudarminto - Sudjono, Budi Santoso Sugihartuti , Rahmi Sunarso, Agus Syahraini Adhiya Lathifah Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri HS Hadi Tri Lestari Tri Wahyu Suprayogi Triagil, Annisa Lita Tyasningsih, Wiwiek Tyasningsih, Wiwiek Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati Wijaya, Andi Yasmin Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Yeni Dhamayanti Yeni Dhamayanti Yonatan Dimascahyo Budianto Yudhana, Aditya Yulianto, Rangga