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Analisis Pembandingan Pola Keanekaragaman Pisang Introduksi Berdasarkan Penanda Fenotipik dengan Penanda RAPD dan Pendugaan Korelasi antara Keduanya terhadap Komposisi Genomiknya Hanik Rohmah Robi'ah; Sobir Sobir; Memen Surahman
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 3 (2005): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.843 KB) | DOI: 10.24831/jai.v33i3.1266

Abstract

Banana accessions introduced from INIBAP Transit Center (ITC) have been studied their diversity revealed on phenotypic and RAPD markers.  Both of them showed different clustering pattern and not fit to genomic composition. In order to know fitness level of clustering pattern both of them, comparison analysis have done to their similarity coefficient matrix, and followed by partial correlation analysis among phenotypic traits, and genomic composition.  The comparison analysis resulting very poor correlation (r = 0.491). Partially correlation analysis among traits, DNA profile, and Musa genomic composition at 95-99% confidence revealed only OPA-18 line 2 related to accessions possessed pure "A" genome, and they were together associated to green color of petiole margins.  OPD-10 line 3, significantly, associated to accessions with genome dominated "B", but there was no correlation between both of them to any characters. These results suggested that some primers and characters may specifically associated with certain.    Key words:  Comparison analysis, Correlation analysis, Introduction bananas, Musa
Pemilihan Karakter Agronomi untuk Menyusun Indeks Seleksi pada 11 Populasi Kedelai Generasi F6 Desta Wirnas; Imam Widodo; Sobir Sobir; Trikoesoemaningtyas Trikoesoemaningtyas; Didy Sopandie
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 34 No. 1 (2006): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.405 KB) | DOI: 10.24831/jai.v34i1.1270

Abstract

High-yielding variety is one of the targets in soybean breeding program. High-yielding variety could be developed through simultaneous selection. Simultaneous selection  using selection index was more efficient  than selection based on an individual trait only.  Objective of  the research was to select agronomic characters for construction selection index  on 11 soybean populations F6 generation based on correlation analysis, path analysis, and heritability.  The correlation analysis  showed that  number of branch,  number of total nod, number of filled pod, number of unfilled pod, number of total pod, and percentage of filled pod have positively and significantly correlated with  yield per plant.  Based on  path analysis, characters number of branch, number of unfilled pod, and number of filled pod have positive direct on yield per plant.   The result also showed that  number  of filled pod has maximum positive direct effect on yield per plant.  Generally, number of total nod, number of filled pod, number of total pod, and percentage of filled pod have higher heritability  than other characters.   Key words: Soybean, correlation, path analysis, heritability, selection index
Karakter Morfo-Fisiologi Daun, Penciri Adaptasi Kedelai terhadap Intensitas Cahaya Rendah , Kisman; Nurul Khumaida; , Trikoesoemaningtyas; , Sobir; Didy Sopandie
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.917 KB) | DOI: 10.24831/jai.v35i2.1317

Abstract

The main objective of this study was to identify the leaf morpho-physiological characters as markers for adaptation of soybean to low light intensity. The field experiment was conducted using split plot design with 3 replications. First factor was soybean genotype consisted of shade tolerant genotypes (Ceneng, Pangrango) and sensitive genotypes (Godek, Slamet). Second factor was low light intensity consisted of: L0 = control (under full sun light), L1 = 5 days under 50% shading (exposed after 21 days after planting/DAP), L2 = 5 days under dark condition (exposed after 21 DAP), L3 = 3 days 50% shading + 5 days sun light (exposed after 18 DAP), dan L4 = 3 days 50% shading + 3 days sun light + 5 day dark conditions (exposed after 15 DAP). The 50% shading was made artificially using black plastic paranet with 50% light transmitted. Results of this study showed that under the conditions of low light intensity, leaf morpho-physiological characters of soybean that could be used as markers for adaptation were leaf area, specific leaf weight, and chlorophyll content. Leaves of shade tolerant genotypes were wider and thinner and also contained higher chlorophyll especially for chlorophyll b than that of shade sensitive genotypes under low light intensity.   Key words :  Low light intensity, tolerant genotype, leaf area, chlorophyll, specific leaf weight
Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 , Lizawati; Roedhy Poerwanto; , Sobir; Iman Rusmana; Tri Muji Ermayanti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.098 KB) | DOI: 10.24831/jai.v35i2.1321

Abstract

Growth of mangosteen essentially depends on its root system.  Therefore, it needs technology to obtain stringer mangosteen root system.  The use of Agrobacterium rhizogenes bacterium is an alternative.  The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.  The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.  The experiment was arranged in completely randomized design with two factorial treatments.  The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).  The results showed that A. rhizogenes  of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.  Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method.   Key words :  Agrobacterium rhizogenes, Garcinia mangostana, inoculation
Daya Gabung dan Heterosis Ketahanan Pepaya (Carica papaya L) terhadap Penyakit Antraknosa*) Siti Hafsah; Sarsidi S astrosumarjo; Sriani Sujiprihati; , Sobir; Sri Hendrastuti Hidayat
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 3 (2007): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.372 KB) | DOI: 10.24831/jai.v35i3.1331

Abstract

This study was conducted to determine the relative importance of general (GCA) and specific (SCA) combining ability and heterosis effects on resistance to C. gloeosporioides in selected papaya genotypes. A half diallel mating scheme of five genotypes of papaya were evaluated in the field. The combining ability analysis revealed that both the additive and nonadditive gene effects were present.  Crosses between the resistance and susceptible genotypes showed intermediate disease reaction to papaya anthracnose disease (PAD) suggesting a polygenic system of resistance to the disease. Highly resistance heterosis of 51.51% at Tajur and 48.71% at Gunung Geulis were expressed in crosses between IPB5 x PB000174.   Key words: Papaya, anthracnose, GCA, SCA, heterosis
Pola Pewarisan Adaptasi Kedelai (Glycine max L. Merrill) terhadap Cekaman Naungan Berdasarkan Karakter Morfo-Fisiologi Daun , Kisman; , Trikoesoemaningtyas; , Sobir; Nurul Khumaida; Didy Sopandie
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 1 (2008): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.816 KB) | DOI: 10.24831/jai.v36i1.1338

Abstract

The objective of this study was to analyze the inheritance pattern of soybean adaptation to shade stress based on leaf morpho-physiological characters. Genetic materials used in this study consisted of 22 plants of low irradiance (LI)-tolerant genotype (Ceneng) and 22 plants of LI-sensitive genotype (Godek); 21 plants of F1 (Ceneng x Godek); and 114 plants of F2 populations (derived from F1). These populations were planted under shading of paranet 50%. The population was arranged in a Randomized Complete Block Design with two replicates. Analysis of inheritance of soybean adaptation involved estimation of heritability (broad sense) and gene action. Results of this study showed that: adaptation of soybean to low light stress based on characters of yield per plant was highly heritable (68% of broad sense) with partial dominant mode of action. Characters of leaf area and specific leaf weight were highly (68% of broad sense) and moderately (48% of broad sense) heritable, respectively, with additive mode of action. Soybean adaptation based on leaf physiological characters (chlorophyll contents) was highly heritable (70% - 86% of broad sense) in epistatic mode of action.   Key words:  Soybean, leaf morpho-physiological character, heritability, gene action
Development of SCAR Marker for Detection of Sex Expression in Papaya (Carica papaya L.) from Several Genetic Backgrounds , Sobir; Sriani Sujiprihati; Evalina C. Pandia
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.875 KB) | DOI: 10.24831/jai.v36i3.1382

Abstract

Papaya plants are hermaphrodite, pistillate, or staminate. Sex inheritance in papaya is determined by a single gene locus with three alleles of M which is dominant for maleness, MH for hermaphrodites and m which is recessive for femaleness. Only fruits from hermaphrodite plants are marketed since they have the necessary commercial characteristics, i.e., they are pear-shaped and have thicker flesh and a smaller internal cavity. Increased papaya yield has been limited mainly by the ratio of female to hermaphrodite (1: 2) plants normally occurring in orchards. This ratio causes great losses to papaya producers. Identification of seedlings sex during nursery stage is of prime iportance. In order to obtain simple DNA markers to identify sex expression in papaya, five SCAR markers of 20-21 primers were utilized. Examination of these markers into 24 genotypes of papaya from 12 populations of different genetic background revealed that pair of primer PKBT-5 had successfully differentiated male and hermaphrodite plants from female plants. Hence, PKBT-5 pair of primer can be utilized as DNA marker for sex expression character identification in papayas.   Key words: Papaya, sex expression, SCAR marker
The Study of Genetic Diversity and Relationships on Carica sp. by Means of Random Amplified Polymorphic DNA (RAPD) Analysis Ahmad Satori; Sriani Sujiprihati; , Sobir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 30 No. 1 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.417 KB) | DOI: 10.24831/jai.v30i1.1425

Abstract

The genus of Carica comprises 21 species, among them three species have been domesticated and cultivated. They are Carica papaya, Carica candamarcencis and Carica monoica. In Indonesia, two species have been cultivated viz. Carica papaya and Carica candamarcencis. In this study, four accessions of Carica papaya species and one accession of Carica candamarcencis species were analyzed using RAPD technique. We successfully amplified a total I of 40 fragments from these DNA genome by using 6 random primers with GC bases content:::: 60% of each primer. The number of fragments of each primer ranged from 5 to 8 averaged 6.7 fragments per primer. Out of total fragments, 90% showed as polymorphic ranged from 5 to 8 on average 6 fragments per primer. A dendogram based on the UPGMA- link method using Nei and Li similarity and Principal Component Analysis (PCA) plot separated the accessions into two main groups, the Carica papaya species on the one side and the Carica candamarcencis species on the other side. The result demonstrated that RAPD analysis was able to reveal genetic difference between Carica papaya and Carica candamarcencis species, as well as genetic diversity in Carica papaya species.
SCAR (Sequence Characterized Amplified Region) Analysis for Pi-b and Pi-ta genes on 28 Genotypes of Rice , Sobir; , Santoso; Hajrial Aswidinnoor; Mukelar Amir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 1 (2005): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.578 KB) | DOI: 10.24831/jai.v33i1.1508

Abstract

Evaluation to blast disease (Pyricularia grisea) resistance was carried out by using two SCAR (Sequence Characterized Amplified Region) markers of Pi-b and Pi-ta blast resistance genes, and spray-inoculation method with 10 races of P. grisea on 28 paddy genotypes, that consisted six wild genotypes of rice. The results revealed that among 28 paddy genotypes, fifteen genotypes carrying both genes including Oryza rufipogon; six genotypes carrying Pi-b genes including O. alta, two genotypes carrying Pi-ta gene, and five genotypes did not possess both gene including O. glumaepatula, O. officinalis, O. latifolia, and O. malapuzhaensis. Based on infection intensity, the evaluated genotypes were vary in responses to different  ten races of P. grisea, indicated that the evaluated genotypes were  vary in carrying Pi genes. Analysis in detail indicated that existence of Pi-ta gene associated with lower infection intensity caused by P. grisea race 063 C.   Keywords:  SCAR markers, rice blast resistance, Pyricularia grisea
SCAR (Sequence Characterized Amplified Region) Analysis for Blast Resistant Evaluation on 12 Genotypes of Rice , Sobir; Harmi Andrianyta; Mukelar Amir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 31 No. 1 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.829 KB) | DOI: 10.24831/jai.v31i1.1526

Abstract

Resistance evaluation to blast disease (Pyricularia grisea) on 12 paddy genotypes was carried out in the green house by using spray inoculated method with race 033 and 041 of P. grisea , and SCAR (Sequence Characterized Amplified Region) marker by using Pib primer pairs. The results revealed that among 12 paddy genotypes were classified into six resistance groups. The first group comprised two genotypes (Jatiluhur and Asahan) having three resistance genes. The second group comprised two genotypes (Oryza malampuzhaensis and O. punctata) having two resistance genes against race 033 and 041. The third group had one resistance gene against race 033, comprised one genotype (Way Rarem). The fourth group comprised one genotype (Danau Tempe) having two resistance genes against 041 race and Pib. The fifth group comprised three genotypes (Kalimutu, Maninjau and Laut Tawar) having two resistance genes against race 033 and Pib. The sixth group comprised two genotypes (Kencana Bali and Cirata) having no resistance gene to blast race 033 and 041, and Pib.  These results indicated that Pib gene did not confer resistance to race 033 and 041 of Pyricularia grisea.  Resistance to race 033 and 041 might be controlled by different resistant gene.   Key words : SCAR, Blast resistant, Rice
Co-Authors , Kisman , Santoso Achmad Baihaki Agung Wahyu Susilo Agus Purwito Ahmad Dadang Ahmad Satori Ahmad Yunus Aji Hermawan Alex Hartana Alex Hartana ALEX HARTANA ALFRED P. MANAMBANGTUA Alifiya Herwitarahman Anas Dinurrohman Susila Andarwening, Freestina Andika Septiana Suryaningsih Aris Purwanto Arisanti, Tiffani Nindya Astri W Wulandari Astri Windia Wulandari Wulandari Awang Maharijaya Azhari, Andi Baiq Arriyadul Badi'ah Bonjok Istiaji Budi Santosa C Hanny Wijaya Chesaria, Nanda DADANG, AHMAD Deden Derajat Matra Delvi Maretta Desta Wirnas Didy Sopandie Diny Dinarti Diyah Martanti, Diyah DONATA S. PANDIN Edi Santosa Edi Santosa Edi Santosa Efendi, D. Efendi, Darda Eka Fatmawati Tihurua Eko Priyantono Ellina Mansyah Entit Hermawan Eny Widajati Erni Suminar Evalina C. Pandia Fusao Motoyoshi Gunawan Gunawan Gunawan Gunawan Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hanifah Nuryani Lioe Hanik Rohmah Robi'ah Harmi Andrianyta Harti, Heri Haryanti, Dyra Helmi, Susan Herry Suhardiyanto I Wayan Budiastra Ika Roostika Imam Widodo Iman Rusmana Imas Sukaesih Sitanggang Inayah Yasmin Kamila Indah Kurniasari Irvan Faizal Is Helianti Islah Hayati J. K. J. Laisina Joko Prasetiyono Juliati S. Darsa Juliet M.E. Mamahit Ketty Suketi Khairiyah, Hayat Khamidi, Thamrin Kisman Kisman Kristianto Nugroho Kurniawan Rudi Trijatmiko Kuswandi Kuswandi La Ode Safuan La Ode Safuan Laksono Trisnantoro Lilik Pujantoro Lina Herlina Lina Herlina Lina Herlina Lizawati . M. Syamsul Maarif Machfud Machfud Mahat Magandhi MANAMBANGTUA, ALFRED P. Mardiana - MARIA BINTANG Marlin Marlin Marlina Mustafa, Marlina Mas’ud, Zainal Alim MATHIUS, NURITA TOURAN Matra, Deden Derajat Meddy Rachmadi Memen Surahman Mien A. Rifai Miftahudin Miftahudin . Muchlis . Muhamad Achmad Chozin Muhamad Syukur Muhammad Arif Nasution Muhammad Syukur Mukelar Amir Munarti Nadila, Dea Nazli, Rizal Sjarief Sjaiful Nazly Aswani Nettyani, Naipospos Ni Made Armini Wiendi Nining, Euis Nobuo Sugiyama Noorrohmah, Siti Nurita Toruan-Mathius Nurul Khumaida Nurwahyuningsih Nurwahyuningsih Olivia Yofananda, Olivia OTIH ROSTIANA PANDIN, DONATA S. Parjanto Parjanto Poetri Agustine Aryawati PRASETIYONO, JOKO Prawestri, Apriliana Dyah Priyanti Priyanti Priyanti Priyanti PURNAMA HIDAYAT Purwono Purwono Putra, Fiqhri Mulianda Putri, Faradila Danasworo Rahmadara, Gemilang Ramadhani Dwi Santoso Ramadhani Dwi Santoso Rd. Selvy Handayani Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Ridwan Setiamihardja Risa Aryantri Ritonga, Arya W Ritonga, Arya Widura Roberdi ,, Roberdi Roedhy Poerwanto Rosmaina Rosmaina S H Hidayat S Noorrohmah Santosa, Budi Santosa, Budi Sarsidi S astrosumarjo Sekar Wulan Prasetyaningtyas Siti Hafsah Soaloon Sinaga SOLIN, NIDA W.N.M. SRI HENDRASTUTI HIDAYAT Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sudarmono Sudirman Yahya Sujiprihati, Sriani Sulistijorini Sunyoto Sunyoto Suparjo Suprayanti Martia Dewi, Suprayanti Martia Surjono Hadi Sutjahjo Suryo Wiyono SYAFRIDA MANUWOTO Syarifah Iis Aisyah Syukur, M Tamami, Djoko TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATIK CHIKMAWATI Tengku Laila Kamaliah Teuku Tajuddin Tias Arlianti Tony Liwang Toruan-Mathius, dan Nurita Tri Budiyanti Tri Handayani Tri Muji Ermayanti Tri Muji Ermayanti Trijatmiko, Kurniawan Rudi Trikoesoemaningtyas Undang, Undang Utami Prawati Wida W. Khumaero Widodo Widodo Willy Bayuardi Suwarno Winarso D. Widodo Winda Nawfetrias Witjaksono Wulan Septiningtyas Kurniajati Wuriandani, Adinda Yoko Mine Yono, Dwi Yudiwanti Wahyu E. Kusumo Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yusnita Sari, Yusnita Zumaidar Zumaidar