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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Manajemen dan Agribisnis Zuriat Jurnal Natur Indonesia Jurnal Ilmiah Aplikasi Isotop Radiasi Jurnal Keteknikan Pertanian Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Buletin Palawija Buletin Penelitian Tanaman Rempah dan Obat Proceeding International Conference on Global Resource Conservation Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Agroteknos Scientific Journal of Informatics Jurnal Agro Bioeksperimen: Jurnal Penelitian Biologi Warta IHP (Warta Industri Hasil Pertanian) BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pertanian Tanaman Pangan Indonesian Food Science and TechnologyJournal Jurnal Penelitian Kelapa Sawit Jurnal Agrotek Tropika Journal of Tropical Crop Science Floribunda Buletin Palma Buletin Penelitian Tanaman Rempah dan Obat Buletin Palawija Agroteksos Jurnal Pusat Inovasi Masyarakat International Journal of Agriculture System Policy Brief Pertanian, Kelautan, dan Biosains Tropika

Sobir Sobir

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB, Darmaga, Bogor 16680, Indonesia

Author-ID : 383388

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 Lizawati, ,; Poerwanto, Roedhy; Sobir, ,; Rusmana, Iman; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Growth of mangosteen essentially depends on its root system. Therefore, it needs technology to obtain stringer mangosteen root system. The use of Agrobacterium rhizogenes bacterium is an alternative. The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root. The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor. The experiment was arranged in completely randomized design with two factorial treatments. The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping). The results showed that A. rhizogenes of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control. Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method. Key words : Agrobacterium rhizogenes, Garcinia mangostana, inoculation

Daya Gabung dan Heterosis Ketahanan Pepaya (Carica papaya L) terhadap Penyakit Antraknosa*) Hafsah, Siti; astrosumarjo, Sarsidi S; Sujiprihati, Sriani; Sobir, ,; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

This study was conducted to determine the relative importance of general (GCA) and specific (SCA) combining ability and heterosis effects on resistance to C. gloeosporioides in selected papaya genotypes. A half diallel mating scheme of five genotypes of papaya were evaluated in the field. The combining ability analysis revealed that both the additive and nonadditive gene effects were present. Crosses between the resistance and susceptible genotypes showed intermediate disease reaction to papaya anthracnose disease (PAD) suggesting a polygenic system of resistance to the disease. Highly resistance heterosis of 51.51% at Tajur and 48.71% at Gunung Geulis were expressed in crosses between IPB5 x PB000174. Key words: Papaya, anthracnose, GCA, SCA, heterosis

Pola Pewarisan Adaptasi Kedelai (Glycine max L. Merrill) terhadap Cekaman Naungan Berdasarkan Karakter Morfo-Fisiologi Daun Kisman, ,; Trikoesoemaningtyas, ,; Sobir, ,; Khumaida, Nurul; Sopandie, Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of this study was to analyze the inheritance pattern of soybean adaptation to shade stress based on leaf morpho-physiological characters. Genetic materials used in this study consisted of 22 plants of low irradiance (LI)-tolerant genotype (Ceneng) and 22 plants of LI-sensitive genotype (Godek); 21 plants of F1 (Ceneng x Godek); and 114 plants of F2 populations (derived from F1). These populations were planted under shading of paranet 50%. The population was arranged in a Randomized Complete Block Design with two replicates. Analysis of inheritance of soybean adaptation involved estimation of heritability (broad sense) and gene action. Results of this study showed that: adaptation of soybean to low light stress based on characters of yield per plant was highly heritable (68% of broad sense) with partial dominant mode of action. Characters of leaf area and specific leaf weight were highly (68% of broad sense) and moderately (48% of broad sense) heritable, respectively, with additive mode of action. Soybean adaptation based on leaf physiological characters (chlorophyll contents) was highly heritable (70% - 86% of broad sense) in epistatic mode of action. Key words: Soybean, leaf morpho-physiological character, heritability, gene action

Development of SCAR Marker for Detection of Sex Expression in Papaya (Carica papaya L.) from Several Genetic Backgrounds Sobir, ,; Sujiprihati, Sriani; Pandia, Evalina C.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Papaya plants are hermaphrodite, pistillate, or staminate. Sex inheritance in papaya is determined by a single gene locus with three alleles of M which is dominant for maleness, MH for hermaphrodites and m which is recessive for femaleness. Only fruits from hermaphrodite plants are marketed since they have the necessary commercial characteristics, i.e., they are pear-shaped and have thicker flesh and a smaller internal cavity. Increased papaya yield has been limited mainly by the ratio of female to hermaphrodite (1: 2) plants normally occurring in orchards. This ratio causes great losses to papaya producers. Identification of seedlings sex during nursery stage is of prime iportance. In order to obtain simple DNA markers to identify sex expression in papaya, five SCAR markers of 20-21 primers were utilized. Examination of these markers into 24 genotypes of papaya from 12 populations of different genetic background revealed that pair of primer PKBT-5 had successfully differentiated male and hermaphrodite plants from female plants. Hence, PKBT-5 pair of primer can be utilized as DNA marker for sex expression character identification in papayas. Key words: Papaya, sex expression, SCAR marker

The Study of Genetic Diversity and Relationships on Carica sp. by Means of Random Amplified Polymorphic DNA (RAPD) Analysis Satori, Ahmad; Sujiprihati, Sriani; Sobir, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 30 No. 1 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The genus of Carica comprises 21 species, among them three species have been domesticated and cultivated. They are Carica papaya, Carica candamarcencis and Carica monoica. In Indonesia, two species have been cultivated viz. Carica papaya and Carica candamarcencis. In this study, four accessions of Carica papaya species and one accession of Carica candamarcencis species were analyzed using RAPD technique. We successfully amplified a total I of 40 fragments from these DNA genome by using 6 random primers with GC bases content:::: 60% of each primer. The number of fragments of each primer ranged from 5 to 8 averaged 6.7 fragments per primer. Out of total fragments, 90% showed as polymorphic ranged from 5 to 8 on average 6 fragments per primer. A dendogram based on the UPGMA- link method using Nei and Li similarity and Principal Component Analysis (PCA) plot separated the accessions into two main groups, the Carica papaya species on the one side and the Carica candamarcencis species on the other side. The result demonstrated that RAPD analysis was able to reveal genetic difference between Carica papaya and Carica candamarcencis species, as well as genetic diversity in Carica papaya species.

SCAR (Sequence Characterized Amplified Region) Analysis for Pi-b and Pi-ta genes on 28 Genotypes of Rice Sobir, ,; Santoso, ,; Aswidinnoor, Hajrial; Amir, Mukelar
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 1 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Evaluation to blast disease (Pyricularia grisea) resistance was carried out by using two SCAR (Sequence Characterized Amplified Region) markers of Pi-b and Pi-ta blast resistance genes, and spray-inoculation method with 10 races of P. grisea on 28 paddy genotypes, that consisted six wild genotypes of rice. The results revealed that among 28 paddy genotypes, fifteen genotypes carrying both genes including Oryza rufipogon; six genotypes carrying Pi-b genes including O. alta, two genotypes carrying Pi-ta gene, and five genotypes did not possess both gene including O. glumaepatula, O. officinalis, O. latifolia, and O. malapuzhaensis. Based on infection intensity, the evaluated genotypes were vary in responses to different ten races of P. grisea, indicated that the evaluated genotypes were vary in carrying Pi genes. Analysis in detail indicated that existence of Pi-ta gene associated with lower infection intensity caused by P. grisea race 063 C. Keywords: SCAR markers, rice blast resistance, Pyricularia grisea

SCAR (Sequence Characterized Amplified Region) Analysis for Blast Resistant Evaluation on 12 Genotypes of Rice Sobir, ,; Andrianyta, Harmi; Amir, Mukelar
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 31 No. 1 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Resistance evaluation to blast disease (Pyricularia grisea) on 12 paddy genotypes was carried out in the green house by using spray inoculated method with race 033 and 041 of P. grisea , and SCAR (Sequence Characterized Amplified Region) marker by using Pib primer pairs. The results revealed that among 12 paddy genotypes were classified into six resistance groups. The first group comprised two genotypes (Jatiluhur and Asahan) having three resistance genes. The second group comprised two genotypes (Oryza malampuzhaensis and O. punctata) having two resistance genes against race 033 and 041. The third group had one resistance gene against race 033, comprised one genotype (Way Rarem). The fourth group comprised one genotype (Danau Tempe) having two resistance genes against 041 race and Pib. The fifth group comprised three genotypes (Kalimutu, Maninjau and Laut Tawar) having two resistance genes against race 033 and Pib. The sixth group comprised two genotypes (Kencana Bali and Cirata) having no resistance gene to blast race 033 and 041, and Pib. These results indicated that Pib gene did not confer resistance to race 033 and 041 of Pyricularia grisea. Resistance to race 033 and 041 might be controlled by different resistant gene. Key words : SCAR, Blast resistant, Rice

Optimasi Pemanfaatan Tanaman Transgenik Sobir, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 29 No. 2 (2001): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Human effort on improving agricultural crops has been expanding very rapidly, and recently by using gene transformation technology it was possible to introduce genes from biologically unrelated organisms into plant genomes. Gene transformation technology, known as transgenic technology, has been successfully introduced gene encoding insecticidal compound from bacteria Bacillus thuringiensis into several crops, for example. Introducing new technology is offering several advantages but also brings several disadvantages however since the technology isImportant for maintaining increase of crop production, it was necessary to optimize utilization of transgenic technology. Key words: Gene transformation, Expression control, Antibiotic resistance

IDENTIFICATION OF CO-DOMINANT DNA MARKER TIGHTLY LINKED TO Tm-2 LOCUS IN TOMATO Sobir, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 27 No. 3 (1999): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Tm-2 adalah gen yang mengendalikan ketahanan terhadap tomato mosaic virus (ToMV) pada tomat (Lycopersicon esculentum) dan berasal dari Lycopersicon peruvianum. Pada penelitian ini digunakan dua galur tomat yang mempunyai latar belakang genetik yang sarna GCR26 dan GCR3 6, akan tetapi keduanya dapat dibedakan oleh adanya gen Tm-2 pada GCR36. Melalui analisis random amplified-polymorphic DNA (RAPD) terhadap dua galur tomat tersebut di atas diketahui adanya primer RAPD yang menghasilkan fragmen spesifik untuk GCR236. Selanjutnya fragmen tersebut sekuennya dianalisis, dan dari hasil analisis sekuen tersebut dibuat sepasang primer sequence characterized amplified region (SCAR) Yang merupakan marka DNA kodominan yang bisa membedakan baik genotipe homozigot gen Tm-2. Oleh karena fragmen dihasilkan terkait dengan Tm-2, maka primer SCAR tersebut merupakan sarana yang dapat digunakan untuk pemulian tanarnan tomat bagi pembentukan varietas tahan virus.

Karakter Fisik dan Kimia Buah Pepaya pada Stadia Kematangan Berbeda Suketi, Ketty; Poerwanto, Roedhy; Sujiprihati, Sriani; Sobir, ,; Widodo, Winarso Drajad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The objective of the experiment was to investigate the physical and chemical characteristics of three stadia of maturity based on a range of peel color from green to yellow or based on percentage of the yellow area of fruit peel (stadium 1 = 25-49 % yellow, stadium 2 = 50-74 % yellow, and stadium 3 = above 75 % yellow) on six genotypes of papaya. Each genotype exhibited different days to maturity for each stadium. The fruits of stadium 1, 2 and 3 for IPB 1 were picked at 130, 135, and 140 days after anthesis (DAA); IPB 10A at 160, 165, and 170 DAA; IPB 1 x PB 174 at 135, 140 and 145 DAA; while PB 174, IPB 1 x IPB 10A and IPB 10A x PB 174 were picked at 140, 145 and 150 DAA, respectively. The results indicated that peel firmness was affected by maturity stage on female fruit of IPB 10A. Maturity stage affected chemical characteristics of papaya included total soluble solids (TSS) content (IPB 10A, female fruit of PB 174, female fruit of IPB 1 x IPB 10A, and female fruit of IPB 1 x PB 174), vitamin C content (hermaphrodite fruit of 10 A, female fruit of IPB 1 x IPB 10A) and juice pH (hermaphrodite fruit of IPB 1). IPB 1 genotype can be harvested at all stadia of maturity stage. Hermaphrodite and female fruit of IPB 10 A, female fruit of PB 174, female fruit of IPB 1 x IPB 10A and female fruit of IPB 1 x PB 174 genotype would be better harvested at stadium 3 of maturity stage. Keywords: Carica papaya, papaya genotype, hermaphrodite fruit, female fruit, fruit quality, fruit maturity stage

Co-Authors , Kisman , Santoso Achmad Baihaki Agung Wahyu Susilo Agus Purwito Ahmad Dadang Ahmad Satori Ahmad Yunus Aji Hermawan ALEX HARTANA Alex Hartana Alex Hartana ALFRED P. MANAMBANGTUA Alifiya Herwitarahman Anas Dinurrohman Susila Andarwening, Freestina Andika Septiana Suryaningsih Aris Purwanto Arisanti, Tiffani Nindya Astri W Wulandari Astri Windia Wulandari Wulandari Awang Maharijaya Azhari, Andi Baiq Arriyadul Badi'ah Bonjok Istiaji Budi Santosa C Hanny Wijaya Chesaria, Nanda DADANG, AHMAD Deden Derajat Matra Delvi Maretta Desta Wirnas Didy Sopandie Diny Dinarti Diyah Martanti, Diyah DONATA S. PANDIN Edi Santosa Edi Santosa Edi Santosa Efendi, D. Efendi, Darda Eka Fatmawati Tihurua Eko Priyantono Ellina Mansyah Entit Hermawan Eny Widajati Erni Suminar Evalina C. Pandia Fadhilah Sitepu, Annisa Fusao Motoyoshi Gunawan Gunawan Gunawan Gunawan Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hanifah Nuryani Lioe Harmi Andrianyta Harti, Heri Haryanti, Dyra Helmi, Susan Hendrastuti Hidayat, Sri Herry Suhardiyanto Hidayat , Sri Hendrastuti I Wayan Budiastra Ika Roostika Imam Widodo Iman Rusmana Imas Sukaesih Sitanggang Inayah Yasmin Kamila Indah Kurniasari Irvan Faizal Is Helianti Islah Hayati J. K. J. Laisina Joko Prasetiyono Juliati S. Darsa Juliet M.E. Mamahit Ketty Suketi Khairiyah, Hayat Khamidi, Thamrin Kisman Kisman Kristianto Nugroho Kurniawan Rudi Trijatmiko Kuswandi Kuswandi La Ode Safuan Laksono Trisnantoro Lilik Pujantoro Lina Herlina Lina Herlina Lina Herlina Lizawati . M A Chozin M. Syamsul Maarif Machfud Machfud Maharijaya, dan Awang Mahat Magandhi MANAMBANGTUA, ALFRED P. Mardiana - MARIA BINTANG Marlin Marlin Marlina Mustafa, Marlina Mas’ud, Zainal Alim MATHIUS, NURITA TOURAN Matra, Deden Derajat Meddy Rachmadi Memen Surahman Mien A. Rifai Miftahudin Miftahudin . Muchlis . Muhamad Syukur Muhammad Arif Nasution Muhammad Syukur Mukelar Amir Munarti Nadila, Dea Nazli, Rizal Sjarief Sjaiful Nazly Aswani Nettyani, Naipospos Ni Made Armini Wiendi Nining, Euis Nobuo Sugiyama Noorrohmah, Siti Nurita Toruan-Mathius Nurul Khumaida Nurwahyuningsih Nurwahyuningsih Olivia Yofananda, Olivia OTIH ROSTIANA PANDIN, DONATA S. Parjanto Parjanto Poetri Agustine Aryawati PRASETIYONO, JOKO Prawestri, Apriliana Dyah Priyanti Priyanti Priyanti Priyanti Priyantono, Eko PURNAMA HIDAYAT Purwono Purwono Putra, Fiqhri Mulianda Putri, Faradila Danasworo Rahmadara, Gemilang Ramadhani Dwi Santoso Ramadhani Dwi Santoso Rd. Selvy Handayani Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Ridwan Setiamihardja Risa Aryantri Ritonga, Arya W Ritonga, Arya Widura Roberdi ,, Roberdi Robi'ah, Hanik Rohmah Roedhy Poerwanto Rosmaina Rosmaina S H Hidayat S Noorrohmah Santosa, Budi Santosa, Budi Sarsidi S astrosumarjo Sekar Wulan Prasetyaningtyas Siti Hafsah Soaloon Sinaga SOLIN, NIDA W.N.M. SRI HENDRASTUTI HIDAYAT SRIANI SUJIPRIHATI Sriani Sujiprihati Sudarmono Sudirman Yahya Sujiprihati, Sriani Sulistijorini Sunyoto Sunyoto Suparjo Suprayanti Martia Dewi, Suprayanti Martia Surjono Hadi Sutjahjo Suryo Wiyono SYAFRIDA MANUWOTO Syarifah Iis Aisyah Syukur, M Tamami, Djoko TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATIK CHIKMAWATI Tengku Laila Kamaliah Teuku Tajuddin Tias Arlianti Tony Liwang Toruan-Mathius, dan Nurita Tri Budiyanti Tri Handayani Tri Muji Ermayanti Trijatmiko, Kurniawan Rudi Trikoesoemaningtyas Undang, Undang Utami Prawati Wida W. Khumaero Widodo Widodo Willy Bayuardi Suwarno Winarso D. Widodo Winda Nawfetrias Witjaksono Wulan Septiningtyas Kurniajati Wuriandani, Adinda Yoko Mine Yono, Dwi Yudiwanti Wahyu E. Kusumo Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yusnita Sari, Yusnita Zumaidar Zumaidar

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