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All Journal Media Kesehatan Masyarakat Indonesia Jurnal Natur Indonesia JURNAL SAINS DAN TEKNOLOGI INDONESIA Journal of Marine Research BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Forestry Research Jurnal AgroBiogen Buletin Iptek Tanaman Pangan EDUKASIA MEDISAINS Bioeksperimen: Jurnal Penelitian Biologi Forum Geografi Jurnal Florea JOURNAL ONLINE OF PHYSICS Jurnal Bioedukasi BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Penelitian Karet Jurnal Pendidikan Biologi SASINDO Science and Technology Indonesia Journal of Language and Literature Jurnal Geologi dan Sumberdaya Mineral (Journal of Geology and Mineral Resources) Jurnal Kesehatan Bakti Tunas Husada Metode : Jurnal Teknik Industri ANNALES BOGORIENSES Al-Madrasah: Jurnal Ilmiah Pendidikan Madrasah Ibtidaiyah Jurnal Medik Veteriner Media Keperawatan Indonesia Bioedusiana: Jurnal Pendidikan Biologi JURNAL Al-AZHAR INDONESIA SERI HUMANIORA Jurnal Riset Kesehatan Poltekkes Depkes Bandung International Journal for Educational and Vocational Studies Jurnal Kesehatan Karya Husada Jurnal Abdimas Madani dan Lestari (JAMALI) Jurnal Geofisika REiLA: Journal of Research and Innovation in Language Jurnal Metaedukasi : Jurnal Ilmiah Pendidikan Lahjah Arabiyah: Jurnal Bahasa Arab dan Pendidikan Bahasa Arab Bioeduca : Journal of Biology Education Journal of Earth and Marine Technology (JEMT) Euler : Jurnal Ilmiah Matematika, Sains dan Teknologi MAHESA : Malahayati Health Student Journal JoMMiT : Jurnal Multi Media dan IT Journal of Applied Sciences, Management and Engineering Technology (JASMET) Muhammadiyah Journal of Geriatric Indonesian Journal of Earth Sciences Journal of Applied Nursing and Health Jurnal Ilmiah Kesehatan (JIKA) Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan QULUBANA: Jurnal Manajemen Dakwah At-Tajdid: Jurnal Ilmu Tarbiyah HEUTAGOGIA: Journal of Islamic Education Tanfidziya: Journal of Arabic Education Makara Journal of Science Jurnal Manajemen, Organisasi, Dan Bisnis Jurnal Abdi Kesehatan dan Kedokteran Investama : Jurnal Ekonomi dan Bisnis Eduvest - Journal of Universal Studies Jurnal Pengabdian Pada Masyarakat Jurnal Teknologi Informasi Jurnal Keperawatan Innovative: Journal Of Social Science Research Sasindo : Jurnal Pendidikan Bahasa dan Sastra Indonesia Transforma: Jurnal Manajemen Journal Economic Management and Business Kapuas Al Itimad: Jurnal Dakwah dan Pengembangan Masyarakat Islam Jurnal Pengabdian Masyarakat Al-Wasatiyah: Journal of Religious Moderation Jurnal Agro Veteriner Jurnal Geosaintek Annales Bogorienses Al-I'timad: Jurnal Dakwah dan Pengembangan Masyarakat Islam
Suharsono Suharsono
Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian, PO Box 66 Malang Telp. (0341) 801468
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Journal : ANNALES BOGORIENSES

 1 
Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz) Susanti, Idha; Suharsono, Suharsono; Widyastuti, Utut; Siregar, Ulfah Juniarti; Tjahjoleksono, Aris
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v21i2.223

Abstract

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media.  This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment.  Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and  half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”.  All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos
Constitutive Expression of Candida antarctica Lipase B (CALB) in Pichia pastoris Using pGAPZα Vector Wahyuni, Febriana Dwi; Fuad, Asrul Muhamad; Suharsono, Suharsono
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.586 KB) | DOI: 10.14203/ab.v20i1.225

Abstract

The CalBsyn gene was previously constructed synthetically to encode Candida antarctica lipase B (CALB). Lipase from CalBsyn gene is slightly different from that of wild type CALB (CALB-wt) where it has three amino acids substitutions at different positions, i.e. V210I, A281E, and V221D, to improve enzyme’s thermostability and catalytic efficiency. The CalBsyn gene was isolated from pJ912-CalBsyn vector by digestion using XhoI restriction enzyme. The CalBsyn gene then was ligated to pGAPZα expression vector and transformed into E. coli TOP10F’ in order to obtain recombinant vector pGAPZα-CalBsyn. The result showed that pGAPZα-CalBsyn recombinant vector was successfully transformed into E. coli TOP10F’ with transformation efficiency of 4.11 x 103 cfu/µg plasmid DNA. The pGAPZα-CalBsyn recombinant plasmid was successfully introduced into Pichia pastoris SMD1168H using electroporation method with transformation efficiency of 1.01 x 102 cfu/µg DNA. Qualitative lipase activity assays showed that transformed P. pastoris secreted recombinant lipase (CALB) and has lipolytic activity; while quantitative lipase activity assays showed that the lipase activity was 63.5 Units/ml in 48 hours. Analysis using SDS-PAGE showed that CALB protein was expressed successfully and the recombinant protein’s molecular size was approximately 45 kDa.
Partial Purification, Characterization, and Application of Extracellular Aspartic Protease from Lactobacillus casei WSP in Producing the Bioactive Peptides with Antibacterial and Antioxidant Activity Solikhin, Akhmad; Mustopa, Apon Zaenal; Suharsono, Suharsono; Putranto, Wendry Setiyadi
ANNALES BOGORIENSES Vol 22, No 2 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2018.v22.n2.47-56

Abstract

   Lactobacillus casei WSP-derived an aspartic protease was sequentially purified by using chromatography gel filtration sephadex G-50. It resulted in a 22.81-fold increase of specific activity (51.5 U/mg) with a final yield of 1.9%. The estimated molecular weight of the purified enzyme was 37 kDa and showed gelatinolytic activity in zymogram assay. The enzyme exhibited optimum activity at 40ºC and pH 6 with casein as the substrate. Enzyme activity was significantly inhibited by pepstatin A (0.5 mM and 1 mM), confirming that this enzyme is a group of aspartic proteases, while other inhibitors such as EDTA, PMSF and iodoacetic acid showed no inhibition effect on the activity of enzyme. The addition of metal ion to the enzyme decreased enzyme activity, indicating the proteolytic enzyme was metal ion- dependent. Denaturant such as DDT tended to increase caseinolytic activity. Furthermore, this enzyme was capable of generating the new peptides from skimmed milk with the size 8 kDa, 10 kDa and 15 kDa. These peptides have potential as antibacterial and antioxidant agents.
Co-Authors AA Sudharmawan, AA Adi Isworo, Adi Adikara, Tatang Santanu Agung Setiabudi Aini, Kuratul Akhmad Solikhin AKIHO YOKOTA Al arif, Mohammad Anam Al Firah, Al Firah ALEX HARTANA Alimuddin, Khasan Ana Quthratun Nada Angga Sugiarto Anik Herminingsih Apon Zaenal Mustopa Arafat, Yasir Arif Rianto Budi Nugroho Aris Tjahjoleksono Arwani Arwani Ashadi Ashar N, Novema Aspariansyah, Aspariansyah Asrul Muhamad Fuad, Asrul Muhamad Astanto Kasno Avellyn Shinthya Sari Aviadi, Dedi Badriah, Liah Baehaqi Bambang Sarwono Bambang Yulianto Budianingsih, Budianingsih Cahyadi, Tedy Agung Caswita Caswita Cici Indriani Dalimunthe damayanti, dini Dani Ramdani, Dani Dessy Adelin Destiana, Destiana Didy Sopandie Diki Muhamad Chaidir Diponegoro, Muhammad Djaka Marwasta Egi, Egi Nuryadin Eko Teguh Paripurno Elfa Cahya Jamiati Endang Surahman Erawati, Erna Fachry Abda El Rahman Faisal Rachman Febriana Dwi Wahyuni Feri, Zefki Okta Ferry Faisal, Ferry Firdaus, Syarifin Fitri Damayanti Fitri Damayanti Fitri Wibowo Fitri, Novi Aryani Gandhi Sutjahjo Gesa Cantya Primatara Gunawan, Heru Hadiarto, Toto Hamim Hamim Hapsari, Agustina Retno Harijani, Nenny Hariyadi, Rahmat Hasan Labiqul Aqil Hermana, Salma Junaina Hidanah, Sri Hutabarat, Madya I, Heriberta Reny Idayati, Tutik Rosita Idha Susanti Iif Rahmat Fauzi, Iif Rahmat Ika Barokah Suryaningsih Ika Mariska IMAM MUSTOFA Irbah, Ariq Nurjannah Ishaq, Rizal Maulana Jayawarsa, A.A. Ketut Kagoya, Tundani Kartawan, Kartawan Karuwal, Ritha Lusian KINYA AKASHI Komul, Marsha Krisdiana Wijayanti Kristanto, Paustinus Edi Kusuma, Amino V.A. Kusumayudha, Sari Bahagiarti Kuswanto, Edi Lulut Dwi Sulistyaningsih Lulut Handayani Maemonah, Maemonah Mahmudah, Uswatun marwoto marwoto Meta, Raharni Rosfiyanti Miftahudin . Mochamad Widjanarko Muhammad Helmi Muhammad Jusuf Muhammad Sirril Wafa Muin, Muhammad Rusli Munasik Munasik Mustafawi, Wike Z. Mustofa, Romy Faisal Mustopa, Apon Z. Mutiara, Frista Mycelia Paradise Nabiila, Alyaa Nainggolan, Adinda Rachel Nasrul, Herni Widiyah Ningrum, Hanis Rachna Noor Faidah, Noor Noorikhsan, Faisal Fadilla Novriyandi Hanif Nugroho, Arif Rianto Budi Nugroho, Arif Riyanto Budi Nur Izzati, Aisyah Nuriyatul Fitriya Nurjanah, Fitri Nuryadin, Egi Oktavianti Oktavianti Palaniappan, Ananda Kumar Pausta Yugianus Pertiwi, Nadira Rasya Polosoro, Aqwin Pramono, Sidik Prasetya, Wandy Murti Prasetyadi, C Prasetyadi, Carolus Prasetyadi, Corolus Pratama, Yudha Agung Pujiastuti, Rr Sri Endang purwoko, devit RADITE TISTAMA Rasyidina, Sumayya Ainur RATIH DEWI HASTUTI Rikoh Manogar Siringoringo Rinaldi Rizal Putra, Rinaldi Rizal Rivai, Muhamad Rizal Rochadi Abdulhadi Rohananingsih, Rohananingsih Rojabtiyah, Rojabtiyah Rondhianto Rondhianto S. Hidayat, S. Safari, Neng Ayu Mustika Sahputra, Ngatno Sahroni, Sahroni Sahupala, Sapiah Samuel Agus Triyanto, Samuel Agus Santika, Wulan Puja Saputra, Muhammad Wahyu Saputri, Niken Anggraini Sri Sari Bahagiarti Kusumayudha Sari Bahagiarti Kusumayudha Sari, Avellyn Shintya Sari, Fatihah Fajar Sarmanu, Sarmanu Sarsidi Sastrosumarjo Septiana, Erni Setiahadiwibowo, Ajimas Pascaning Shohifaha , Salsabila Sholihin, Achmad Simanjuntak, Veronica Sirait, Budi Chrismanto Sirojuddin, Wildan Siti Zulaeha, Siti sodiq, alwi mahardhika Soekarta, Rendra Solly Aryza Sri Hidanah Sri Langgeng Ratnasari SRI RAHAYU Sri Sumarni Sugiyarto, Angga Sulistiani, Erina Sulistyaningsih, Yohana C Sulistyowati, Dina Indrati Dyah Sunarko Sunarko Supena, Ence Darmo Jaya Supranianondo, Koesnoto Susanto, Abiyyu Naufal Sutarto Sutarto Sutrisno, Sutrisno Suyanta Suyanta Ta'adi, Ta'adi Teuku Tajuddin Tita Damayanti Lestari Titik Sudartinah Titin Handayani Trisnaningrum, Niken Trisnowati, Yuni Dwi Trustinah Trustinah Ulfah Juniarti Siregar Utut Widy Astuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Vita Meylani Wahyu Hidayat Wamiliana Wamiliana Wendry Setiyadi Putranto Wening Enggarini Widya Paramita Lokapirnasari Widyastuti, Arum WIJAYANTO WIJAYANTO Winarso, Widyo Wiwik Misaco Yuniarti YanRiska Venata Sembiring, YanRiska Venata Yeni Yulistanti Yohana Noradika Maharani Yulianto, Bernawan Yuniati, Ratna YUSMANI PRAYOGO Zainal Muttaqin zulfitra, Zulfitra