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Journal : Life Science and Biotechnology

The HEMOLYSIS ACTIVITY OF BACTERIA ISOLATS FROM PELANGI FOREST OF IJEN GEOPARK Arimurti, Sattya; Utarti, esti; sutoyo, sutoyo; siswanto, siswanto; Ayuningtyas, Tantri Raras
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44097

Abstract

Ijen geopark is one of Indonesia's geoparks, which is located in East Java. A total of 153 bacteria have been isolated from Pelangi Forest, which were then given the isolate code IHP. These bacterial isolates can be utilized in industries, including organic matter decomposer agents, plant biocontrol agents, and probiotics. To ensure these bacteria are safe to use in various fields, they must be non-disease-causing (non-pathogenic). Safety evaluations based on the hemolysis reactions offer simple tests to ease the analysis of potential pathogenic bacteria. The study aimed to evaluate the safety of bacterial isolates from Pelangi Forest for their hemolysis reactions. The hemolysis test was conducted using blood agar media, from which isolates with a negative (λ) reaction. Based on the results of hemolysis tests, 30 out of 153 bacterial isolates (19.60%) were found to be negative reactions. These bacteria are safe to proceed with for potential analysis.
Screening Ethanol-Producing Yeast Origin from Pineapple Honey Ananas comosus (L.) Merr var Quenn Sutoyo, Sutoyo; Siswanto, Siswanto
Life Science and Biotechnology Vol. 1 No. 1 (2023): May
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i1.39560

Abstract

The availability of fossil-based fuels will gradually decrease while the amount required increases. This has prompted research to find alternative energy sources, including bioethanol. Bioethanol, as an alternative energy source, can be used as a transportation fuel better than fossil-based fuels because ethanol has a high octane number, laminar velocity and heat of vaporisation. Meanwhile, the level of carbon and hydrocarbon emissions is low so it can increase combustion efficiency in vehicles. In addition, the use of bioethanol as fuel is environmentally friendly and renewable. Ethanol can be produced through a fermentation process using ethanol-producing yeast. Pineapple honey is known for its high sugar and other nutrient content, so it is supposed that many types of yeast can grow well in it. This study aims to isolation and screening of bioethanol-producing yeast from Pineapple Honey. This study was carried out by yeast isolation method, primary yeast screening, secondary yeast screening, and characterisation of selected yeast isolates. Data on ethanol production by yeast isolates were analysed by ANOVA with α=0.05 using the R studio program. This primary isolation and screening phase found eight yeast isolates capable of fermenting glucose and producing acid and gas in the test medium. Test ethanol production in DPE medium with 18% glucose content on all isolates showed that all isolates could produce ethanol with a range of 4.20-10.35%. Isolates of IK4 showed the highest ethanol production ability (10.35%) (p<0.5%) in significant contrast to the other seven isolates. Based on macroscopic and microscopic morphological characterisations, IKM 4 isolates show similarities to the genus Saccharomyces Keywords: ethanol, pineapple, yeasts
Co-Authors A. F., Elisabeth Abdur Rahim, Abdur Agi Ginanjar Agnes Putri Astuti Agustian Agustian Agustina Nurhayati Aimmann J, Revaldy Alexandrea Tasia Benita Widyasari Ama, Karolus Asan Boli Amir Hamzah Amrullah Amrullah Anang Priyanto Anita Trisiana, Anita Aprilia, Miftah Aprizal Zainal Ardiansyah, Bagas Raka Arief Nugroho, Danang Arien Heryansyah Arimbi, Vina Astri Sumiati Astutik Astutik Astutik, Astuttik Aswat, Amarilla Auzar Syarif Ayuningtyas, Tantri Raras Azhar Azhar Azifah, Nur Bambang Siswanto Bambang Widiyatmoko Barus, Robertus Cahyarani, Elvina Chusnul Arif Damayanti, Elok dedi irawan Demus, Niko Devara, Muhammad Ryan Dewi Hayati, PK DEWI SARTIKA Dewi, Amanda Kusuma Dhone, Emiliyani Meo Diah, Muhammad Duka, Ngailu Beko Dulloh, Zenda Junai Asad Dwi Hanto Edyson Indawan Effendy, Fauzan Ema Butsi Prihastari Ratna Widyaningrum, Ema Butsi Prihastari Erizal Erizal Esti Utarti Etti Swasti Fahrur Rozi Farida Sari, Ama FARIDA, AMA Faustina, Grace Safara Ferriawan Yudhanto, Ferriawan Firmansyah, Denny Ghudi, Enos Magho Gustian Gustian Haikal Akhmad, Fadel Haikal Muhammad, Arsya Halimah, Noer Handari, Linda Wahyu Hasdifa, Abu Rizal Hasmiandy Hamid Hendiyani, Dina Hidayat, Cecep Taupik Hidayat, Febrian R Hidayati Karamina I Made Indra Agastya IBNUL QAYIM Indra Dwipa Indri Kusumaningrum, Fitria Irfan, Dhia Kamal Ishak, Yanuar Jalil, Muhamad Jamhari Jamhari Jehani, Hironimus Jumadi Jumadi Kanangahu, Mercy M Kleor, Fransesko Rudianto Koen Irianto Uripan Kuswandi Kuswandi Laksono Trisnantoro Lalo, Yakub Didimus Liong, Octafiandy Christian Listyo Yudha Irawan Lukmanul Hakim Manang, Aleksius Pongu Lima marwoto marwoto Maryanti, Siti Nur Matheus Kamdiono, Matheus Maulana Ibrahim Rau, Maulana Ibrahim Maulana, Eko Megawati, Rina Mentari, Sekar Miftah Tedy gautama, Miftah Tedy Gautama Muhammad Nurdin Muhammad Sahal Murdiono, Jatmiko Musliar Kasim Muzakki, M Harir Nalwida Rozen Nasdeko, Gandha Ndjurumana, Erlon Nofiyanti, Fifi Nome, Erton Nora H. Pandjaitan Novita, Vivi Tria Nur Oktavia, Bella Nuriman Nuriman, Nuriman Oktafiani, Pradyta Galuh P.K. Dewi Hayati Patimah, Siti Patimah Prakoso, Dwi Bangkit Prasetyaningsih, Bernadetta Ika Purwanto, M. Y. J. Putra, Heriansyah Putri Zafira, Aura Qintana, Muhammad Raka Rafi Aji, Prihandoko Rizqi Rahmawan, Rama Zaky Ramadhanis, Zainab Ricky Indri Hapsari Riduansyah, Muhammad Iqbal Rika Puspita Sari Rizal Ibrahim, Muhammad Rudiyanto Rudiyanto, Rudiyanto S Siswanto Sadewo, Titon Safri, Hayanuddin Sagita, Wilda Nurfi Dera Saifudin Saifudin Saktioto Saktioto Salman, Salwa Nabila SANGAD, SANGAD Santika, Cut Santoso, Apri Saptomo, S. K. Sattya Arimurti Setiawan, Ariyono Sholikha, Devita Eka Zulfiatus Simanjorang, Bernando Agung Saputra Siti Supeni Slamet, Alim S Sobang, Adrianus Janga Sri Astuti Sri Hidayati Sri Murniyanti Sri Umi Lestari Sugiaryo, Sugiaryo Suharyono Suharyono Sujatmika, Sujatmika Sumiati, Astri Sumiati, Astri Susilo Ribut Anggarbeni Sutomo Sutomo Sutralinda, Dety Syahbani, Toha Syahruddin Syahruddin Teguh Budi Prasetyo Theresia Sumei, Theresia Titiek Islami Titis Adisarwanto, Titis Tri Sudibyo Tridaryanto, Anto Utami, Anisa D Utomo, Tri Rizki Uyun, Nur Rohmatul Vincent Vincent, Vincent WAZIN WAZIN, WAZIN Widi Ariyawan, Krisna Widowati Wilhelmina Medho Kota, Wilhelmina Medho Yastutik, Yastutik Zainuddin Iba Zuhrotun, Zuhrotun Zulfikar Zulfikar