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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Indonesia Medicus Veterinus Farmaka Majalah Kedokteran Bandung Jurnal Kesehatan Masyarakat Althea Medical Journal Journal of Biomedicine and Translational Research Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Kesehatan Andalas Folia Medica Indonesiana Indonesian Journal of Pharmaceutical Science and Technology Jurnal Farmasi Klinik Indonesia WMJ (Warmadewa Medical Journal) Global Medical and Health Communication Indonesian Journal of Science and Technology Jurnal Kebidanan The Indonesian Biomedical Journal IDJP (Indonesian Journal of Pharmaceutics) Jurnal Kedokteran Gigi Universitas Padjadjaran Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) Journal of Medicine and Health Molecular and Cellular Biomedical Sciences (MCBS) JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Pharmacology and Clinical Pharmacy Research PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Informatika Kaputama (JIK) Jurnal Ners Jurnal Ilmiah Farmako Bahari Jurnal Ilmu Faal Olahraga Indonesia Narra J Jurnal Pengabdian Olahraga Masyarakat Journal of Applied Nursing and Health JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Molekul: Jurnal Ilmiah Kimia Journal of Tropical Pharmacy and Chemistry Journal of Medicine and Health

Ronny Lesmana

Department Of Anatomy, Physiology And Biology Cell, Faculty Of Medicine, Universitas Padjadjaran, Sumedang 45363, Indonesia

Author-ID : 716099

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Determination and Purification of Ricin Protein from Ricinus communis L. Seeds using CLC (Column Liquid Chramotagoraphy) and FPLC (Fast Protein Liquid Chramatography) Irma Erika Herawati; Ronny Lesmana; Jutti Levita; Anas Subarnas
Indonesian Journal of Pharmaceutical Science and Technology Vol 8, No 3 (2021)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v8i3.35454

Proteins found in all biological systems, from prokaryotes to eukaryotes. Ricin, one of the most toxic substances known isolated from Ricinus communis L. seeds, is a heterodimeric two-domain polypeptide protein that includes chain A (30 kDa) and chain B (35 kDa) linked by a disulfide bond. Ricin binds to cells by the B chain and is then internalized. Ricin has been reported as a potential chemical for cancer treatment. The measurement of protein concentration in an aqueous sample is an important assay in biochemistry research. However, so far, the quantification of ricin protein is not much reported. In this study, the quantification ricin protein extracted from R. communis L. seeds originated from Nganjuk, East Java, Indonesia. The techniques used column liquid chromatography (CLC) and fast protein liquid chromatography (FPLC), followed by quantification protein content used Bradford method. Results showed that all techniques positively confirm the presence of ricin protein. Ricin protein content were 0.171±0.021 mg/mL and 0.382±0.023 mg/mL using CLC and FPLC respectively. This study might contribute to understanding the biological and chemical properties of the ricin protein of R. communis L. seeds. Ricin protein content measured with FPLC were higher than CLC. Keywords: Ricin, Ricinus communis,CLC, FPLC.

Efek Astaxanthin dan Latihan Teratur terhadap Pola Stres Oksidatif Pria Setelah Aktivitas Berat Nova Sylviana; Hanna Gunawan; Ronny Lesmana; Ambrosius Purba; Ieva B. Akbar
Indonesian Journal of Clinical Pharmacy Vol 6, No 1 (2017)
Publisher : Universitas Padjadjaran

Aktivitas fisik berat meningkatkan senyawa oksigen reaktif dalam tubuh yang diketahui dengan mengukur kadar malondialdehid (MDA) dari hasil proses lipid peroksidase yaitu kerusakan oksidatif pada biomolekul lipid akibat reaktivitas senyawa oksigen reaktif (SOR), namun pola perubahan kadar MDA plasma seseorang setelah beraktivitas fisik berat masih belum dipahami. Antioksidan potensial seperti astaxanthin dan latihan teratur diduga dapat memengaruhi pola perubahan kadar MDA tersebut. Untuk itu dilakukan penelitian eksperimental dengan subjek 15 orang pria terlatih dan 15 orang pria tidak terlatih anggota sebuah pusat kebugaran di Bandung, usia 18–25 tahun yang terbagi menjadi kelompok yang mendapatkan suplemen astaxhantin atau plasebo selama satu minggu secara buta ganda. Setelah pemberian suplemen atau plasebo, setiap kelompok melakukan tes aktivitas anaerobik berat. Dilakukan pengukuran MDA rerata (mmol/mL) sebelum dilakukan tes, langsung setelah tes, 6 jam setelah tes, dan 24 jam setelah tes. Data yang dianalisis menggunakan uji ANOVA diikuti uji Duncan menunjukkan bahwa sebelum tes keempat kelompok mempunyai rerata yang hampir sama, sementara rerata MDA yang diperiksa pada setelah tes pada kelompok pria terlatih dengan suplementasi astaxanthin memiliki rerata MDA terendah, sementara yang tertinggi pada kelompok pria tak terlatih dengan plasebo (p<0,05). Perubahan rerata MDA pada setiap kelompok menunjukkan pola dinamis sama yaitu meningkat tajam langsung setelah tes latihan fisik, mulai mengalami penurunan pada jam ke–6 dan kembali ke rerata awal pada jam ke–24 (p<0,05), kecuali pada kelompok pria tak terlatih dengan plasebo meningkat 2 kali dari nilai awal. Hal ini menunjukkan astaxanthin dan latihan mempunyai efek positif terhadap peningkatan kadar MDA namun tidak memengaruhi pola perubahannya setelah aktivitas fisik berat.Kata kunci: Astaxanthin, latihan, malondialdehid The Effect of Astaxanthin and Regular Training on Dynamic Pattern of Oxidative Stress on Male under Strenuous ExerciseAbstractStrenuous physical activity will induce higher Reactive Oxygen Species (ROS) level in human body that can be measured by serum malondialdehyde (MDA) level. Malondialdehyde is product of lipid peroxidation process that defined as oxidative damage of lipid biomolecule by reactivity of reactive oxygen species. Still, the dynamic pattern of malondialdehyde (MDA) level under strenuous exercise is not fully understood. Potent antioxidant such as astaxanthin and training may altered the level of MDA. Thus, the purpose of this study is to understand the effect of astaxanthin to MDA dynamic pattern on training male after strenuous physical activity. It was a double blind, experimental study, conducted on thirty young male age, divided into untrained and trained groups. Supplement astaxanthin was given to 15 subject as well as plasebo for one week. After supplementation, subjects were tested with anaerobic strenuous physical activity. The values were analyzed with ANOVA test followed by Duncan test showed that in every group, mean of MDA before test was similar, started increasing significantly after test, began decreasing at 6th hour post test and back to baseline at 24th hour post test (p<0.05), except for group of untrained male with plasebo, the value still increase twice from baseline. The lowest mean of MDA was found on group of trained male with astaxanthin supplementation and the highest was found on group of untrained male with placebo (p<0.05). These findings support that astaxanthin and training might have positive effect to oxidative stress condition without altered its dynamic pattern in male after strenuous physical activity.Keywords: Astaxanthin, malondialdehyde, training

Role of Herbal Plants on Autophagy Susanti Husen; Ronny Lesmana; Hanna Goenawan; Setiawan Setiawan
Indonesian Journal of Clinical Pharmacy Vol 9, No 2 (2020)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15416/ijcp.2020.9.2.179

Autophagy is a biomolecular system known to play an important role in cell growth, development as well as defense mechanisms, and is also a physiological approach towards homeostasis maintenance. This phenomenon is an intermediary pathway in the pathogenesis and therapy of various disease types, and is currently a new treatment route in the medical world. Hence, it is necessary to deeply understand the regulations, to prepare a treatment strategy in the future. Conversely, there is a high utilization of drugs, in line with the numerous disease cases in the community. Some limited chemical forms have large but necessary side effects, including anticancer agents. The high incidence of resistance and polypharmacy has been implicated in adverse drug interactions, and exorbitant costs, thus the initiative to return back to natural means. Furthermore, herbal medicines have been used in treatments for thousands of years, prior to the discovery of modern medicine. The various types previously studied have been highly beneficial in various disease cases. This review on researches within the last 5 years identified some herbal research articles in the aspect of autophagy, which was then summarized in a table form. Meanwhile, autophagy has been used for cancer therapy, and many plants have the potential to be therapeutic for various diseases through this pathway. Hence, improving related knowledge adds a new dimension to herbal medicine application in the pharmaceutical industry.Keywords: Autophagy, herbal, cancer, metabolic diseases, liver injury, neurological disorderPeran Berbagai Tanaman Herbal pada AutofagiAbstrakAutofagi adalah sistem biomolekuler sel yang berperan penting dalam pertumbuhan sel, perkembangan dan mekanisme pertahanan, dan merupakan sebuah cara fisiologis untuk mempertahankan homeostasis. Autofagi digunakan sebagai jalur perantara untuk patogenesis dan terapi berbagai jenis penyakit, dan saat ini autofagi telah menjadi jalan pengobatan baru di dunia medis sehingga regulasi jalur autofagi harus dipelajari lebih dalam untuk mempersiapkan strategi pengobatan di masa depan. Di sisi lain, tingginya pemanfaatan obat-obatan sejalan dengan banyaknya kasus penyakit yang muncul di masyarakat. Beberapa obat kimia memiliki efek samping yang besar tetapi jumlahnya terbatas, misalnya pada obat antikanker. Selain itu, tingginya kasus resistensi obat dan polifarmasi menyebabkan interaksi obat yang merugikan dan biaya obat yang tinggi. Oleh karena itu, pengobatan kini telah kembali ke alam. Herbal telah digunakan sebagai pengobatan selama ribuan tahun sebelum pengobatan modern ditemukan. Berbagai jenis herbal telah dipelajari dan memiliki manfaat yang baik dalam berbagai kasus penyakit di masyarakat. Pada artikel review ini, ditelaah artikel-artikel penelitian herbal yang mengambil jalur autofagi dan hasilnya dirangkum dalam bentuk tabel. Studi yang dilakukan dalam kurun waktu 5 tahun terakhir dimasukkan dalam review ini. Sampai sekarang, autofagi telah lebih banyak digunakan untuk terapi kanker dibandingkan yang tanpa kanker. Banyak tanaman herbal yang memiliki potensi untuk menjadi terapi berbagai jenis penyakit melalui jalur autofagi. Pengetahuan tentang autofagi menambah dimensi baru pada pengobatan herbal dan dapat diterapkan dalam industri farmasi.Kata kunci: Autofagi, gangguan metabolisme, gangguan saraf, herbal, kanker, kerusakan hati

Pengaruh Krim Asam Traneksamat terhadap Pembentukan Keriput dan Kadar Matriks Metaloproteinase-1 pada Mencit (Mus Musculus) Jantan Galur Balb/c yang Dipajan Sinar Ultraviolet B Andrew Citrawan; Oki Suwarsa; Hendra Gunawan; Sudigdo Adi; Ronny Lesmana; Achadiyani Achadiyani; Sunaryati S. Adi
Indonesian Journal of Clinical Pharmacy Vol 8, No 2 (2019)
Publisher : Universitas Padjadjaran

Pemberian asam traneksamat (AT) per oral telah terbukti dapat mengurangi keriput yang diinduksi oleh kulit kering pada mencit. Namun, dosis oral yang setara terlalu besar untuk digunakan pada manusia dalam jangka panjang karena dapat menimbulkan toksisitas, sehingga perlu dicari sediaan alternatif lain secara topikal seperti sediaan krim. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian krim AT terhadap pembentukan keriput pada kulit mencit jantan galur Balb/c yang dipajan sinar ultraviolet B (UVB). Penelitian dilakukan terhadap 24 ekor mencit di Laboratorium Farmakologi dan Terapi serta Laboratorium Sentral Universitas Padjadjaran pada bulan Februari hingga Mei 2018. Mencit dibagi secara acak ke dalam empat kelompok, yaitu satu kelompok kontrol (P0) hanya mendapatkan pajanan sinar UVB tanpa krim AT dan tiga kelompok perlakuan (P1, P2, dan P3) mendapat pajanan sinar UVB dan diberikan krim AT dengan konsentrasi masing-masing 3%, 4%, dan 5%. Setelah 10 minggu, dilakukan penilaian kondisi keriput pada kulit punggung mencit berdasarkan metode Bisett, dilanjutkan dengan biopsi kulit punggung mencit untuk pemeriksaan kadar matriks metaloproteinase-1 (MMP-1) dengan teknik western blot (WB). Diperoleh perbedaan nilai rata-rata skor keriput yang bermakna sebesar 2,1±0,105 pada kelompok P0, 1,1±0,167 pada P1 dan P2, serta 1,3±0,211 pada P3 (p=0,005). Diperoleh pula nilai rata-rata kadar MMP-1 yang bermakna, yakni sebesar 0,75±0,08 pada kelompok P0, serta 0,54±0,033, 0,40±0,052, dan 0,54±0,072 pada P1, P2, dan P3 secara berturut-turut (p=0,008). Berdasarkan hasil tersebut, dapat disimpulkan bahwa pemberian krim AT mampu memperlambat pembentukan keriput dan menurunkan kadar MMP-1 pada kulit punggung mencit jantan galur Balb/c yang dipajan sinar UVB.Kata kunci: Keriput, krim asam traneksamat, matriks metaloproteinase-1, sinar ultraviolet B The Effect of Tranexamic Acid Cream on Wrinkle Formation and Matrix Metalloproteinase-1 Levels on Male Balb/c Mice Skin Exposed to Ultraviolet B RadiationsAbstractOral tranexamic acid (TXA) has been proven to ameliorate wrinkle induced by skin dryness in hairless mouse. However, the equal human oral dose is too high and can induce toxicity if used in long term, and study of topical preparations for wrinkle treatment is limited. Therefore, this study was conducted using topical preparations as an alternative for oral treatment to examine the effects of TXA cream in wrinkle formation. Four weeks old of twenty-four male Balb/c mice, divided into four groups, then 3%, 4% and 5% TXA cream were administered on the back skin of mice in each group shortly after ultraviolet B (UVB) exposure, except for control group that only exposed to UVB lights without given any TXA creams. Wrinkle formation and matrix metalloproteinase (MMP-1) level were observed after 10 weeks of treatments. There were significant differences of wrinkle score, with mean value were 2.1±0.105 for control group, 1.1±0.167 for 3% and 4% groups, and 1.3±0.211 for 5% group (p=0.005). There were also significant differences of MMP-1, with mean value were 0.75±0.08 for control group, 0.54±0.033, 0.40±0.052, and 0.54±0.072 for 3%, 4% and 5% group, respectively (p=0.008). Based on significant differences of wrinkle score and MMP-1 level, it can be concluded that administration of TXA cream may able to delay wrinkle formation and reduce the MMP-1 level on the mice skin exposed to ultraviolet B.Keywords: Matrix metalloproteinase-1, tranexamic acid, ultraviolet B, wrinkle

Efek Penggunaan Probiotik Topikal Terhadap Ekspresi MMP-13 dan Kolagen III pada Lapisan Dermis Tikus yang Dipapar Sinar Ultraviolet-B Vita M. Tarawan; Anissa Anissa; Endang Sutedja; Ronny Lesmana
Indonesian Journal of Clinical Pharmacy Vol 5, No 2 (2016)
Publisher : Universitas Padjadjaran

Proses penuaan kulit terjadi karena kombinasi penurunan kapasitas proliferasi sel-sel kulit, berkurangnya sintesis matriks dermis, dan peningkatan ekspresi enzim yang mendegradasi matriks kolagen. Penelitian ini merupakan eksperimental laboratorik, menggunakan 24 ekor tikus jantan galur Sprague-Dawley sebagai objek penelitian. Pada kelompok perakuan, kulit dorsal kedua kelompok dicukur, kemudian diberikan paparan sinar UVB seminggu tiga kali selama 4 minggu dengan dosis total penyinaran 840 mJ/ cm2. Kulit kelompok perlakuan diolesi krim Lactobacillus plantarum FNCC 0020 yang tidak bereplikasi, sehari 2 kali dengan komposisi aplikasi topikal koloni 247,27x107, sedangkan kelompok kontrol tidak diolesi apapun. Hasil penelitian diperoleh, baik intensitas, distribusi, maupun histoskor MMP-13 antara kelompok perlakuan dan kelompok kontrol tidak menunjukkan ada perbedaan yang bermakna (p>0,05). Intensitas kolagen III baik pada kelompok perlakuan maupun kontrol semua mempunyai derajat kuat, sedangkan distribusi kepadatan kolagennya paling rendah 20–50% dan tertingginya di atas 80%. Perbandingan distribusi kepadatan kolagen III secara statistik sangat bermakna (p<0,01). Pemberian krim topikal L. plantarum FNCC 0020 meningkatkan ekspresi distribusi kepadatan kolagen III namun tidak menurunkan ekspresi MMP-13. Kata kunci: Lactobacillus plantarum, kolagen, (matrix metalloproteinases) MMP13 Effect of Topical Probiotic on MMP-13 and Collagen III Expression in The Dermis Layer of Male Rats Irradiated with Ultraviolet-B Abstract Nowadays, there is a big interest in the use of topical probiotic preparations for skin health. One of the probiotics therurapeutic benefits is used as anti-aging. During aging, there is stimulation of activator protein-1 (AP-1) which is a transcription factor that inhibits the production of collagen and AP-1 supports the breakdown of collagen by enzymes called matrix metalloproteinases (MMPs). As administration of oral Lactobacillus plantarum could inhibit skin aging by lowering the activity of MMP, so the collagen degradation can be derived so probably topical use of Lactobacillus plantarum may give more prominent effects. We used 24 male rats Sprague-Dawley strain as research objects. This study was divided into two groups, the treatment and control groups. The shaved dorsal skin of rats were irradiated with UVB three times a week for 4 weeks with total irradiation dose of 840 mJ/cm2. Skin cream, containing 247.27x107 CFU non-replicating Lactobacillus plantarum FNCC 0020, was smeared on the treatment group, two times daily, whereas the control group did not receive any treatment. Skin biopsies were done at the end of the study for examination of MMP-13 and collagen III expressions. Intensity, distribution, and histoscore of MMP-13 between the treatment and the control group showed no significant difference (p>0.05). The treatment group showed a significant different in the intensity of collagen III with the density distribution of 20–50% and the highest density was 80% (p<0.01). Administration of topical cream L. plantarum FNCC 0020 increased the expression of collagen III density distribution, but not the MMP-13 expression.Keywords: Collagen, Lactobacillus plantarum, (matrix metalloproteinases) MMP13

Ethionamide Modulates of Thyroid Stimulating Hormone, Thyroxine, and Triiodothyronine Levels in White Ratstimulating Hormone (TSH), Thyroxine (T4), and Triiodothyronine (T3) Serum are potentially modulated Level by Ethionamide in Rats ronny lesmana; Fani Rahma Yenita; Prayudi Santoso; Hanna Goenawan; Arto Yuwono Soeroto; Yuni Susanti Pratiwi; Nova Sylviana; Iwan Setiawan; Vita Murniati Tarawan; Unang Supratman
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.585 KB)

Multidrug-Resistant Tuberculosis (MDR-TB) infection is not solely caused serious impact for patient and cause long recovery process. Multidrug-Resistant Tuberculosis needs specific treatment approach using second-line TB drugs. Ethionamide as one of medication used in MDR-TB are known to cause greater side effects compared to the first-line drugs. One of side effect like hypothyroidism is remain unclear. There is limited study about role of ethionamide cause hypothyroidism. In this present study, 24 male, wistar rats were divided into three groups: ethionamide group; positive control positive (propylthiouracil) and negative control group (received distilled water only) and treated accordingly for 12 week. Under ketamine and xylazine anesthesia, blood sample were collected from retroorbital vein at 8 and 12 week, put in EDTA tube and stored -20Ë%C until use. After last sampling, rat were sacrificed using CO2 chamber. Our data showed that thyroid stimulating hormone (TSH) levels in ethionamide group is increased from 8 weeks to 12 weeks significantly, but there is no significant changes in control groups. Thyroxine (T4) levels is trend decreased from 8 weeks to 12 weeks compared to control groups. There is no change of Triiodothyronine (T3) levels. Effect of ethionamide on thyroid hormone (T3 and T4) levels was not prominent and there were no significant changes observed. Interestingly, we observed an increase of TSH level after ethionamide treatment. Taken together, 12 week treatment of ethionamide tablet might potentially modulate thyroid hormone (T3 and T4) levels that is reflected from an increase of TSH levels in the plasmas feed back negative mechanism.

Effect of Specific Formulated Feed to Alter the Glucose, Triglycerides and Total Cholesterol Level in Rat (EFEK FORMULASI PAKAN TIKUS TERHADAP PERUBAHAN KADAR GLUKOSA, TRIGLISERIDA DAN KOLESTEROL TOTAL) Jihan Mudrika Rahmi; Ronny Lesmana; Hana Goenawan; Setiawan Setiawan; Vita Murniati Tarawan; Okta Wismandanu; Unang Supratman
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Understanding the molecular mechanism of type 2 diabetes mellitus using human sample is very difficult, therefore utilization of animal model can be the solution for studying diabetes mellitus. However, there is still limited option of the animal model that can describe the process of type 2 diabetes mellitus. The aim of this study is to develop a formulated feed with increased glucose, triglycerides, and total cholesterol as characteristics of type 2 diabetes mellitus. This study was done using a descriptive method and experimental study design for 8 weeks. Total 9 male and 12 female Wistar rats were randomly divided into 3 groups: 1 control group and 2 treatment group (formula 1: high carbohydrate, formula 2: high fat). The increase of delta mean body weight was higher in the group given formula 1 for both male and female rats (f: 52g, m: 71.67g). The glucose level was increase from 4th- 8th week in both gender of rats and higher in the group given formula 1 of female rats (4W: 221.13 mg/dl; 8W: 249.83 mg/dl). The triglycerides level was increased from 4th to 8th week and higher in group given formula 2 either male (4W: 37.42 mg/dl; 8W: 58.92 mg/dl) but not in female rats (4W: 76.78 mg/dl; 8W: 71.22 mg/dl). The cholesterol total level also increased in male (4W: 93.05 mg/dl; 8W: 282.5 mg/dl) and female (4W: 101.58 mg/dl; 8W: 227.17 mg/dl) rats and higher in the group given formula 1. In conclussio, either high carbohydrate or high fat pellet showed potential capability to increase the glucose, triglyceride and total cholesterol level especially in female rats.

DMBA-induced Modulate Estrogen Receptors α and β Breast Cancer’s Animal Model Aziiz Mardanarian Rosdianto; Ahmad Kurniawan; Julia Windi Gunadi; Isa Mahendra; Iwan Setiawan; Hanna Goenawan; Nova Sylviana; Yuni Susanti Pratiwi; Mas Rizky Anggun Adipurna Syamsunarno; Roro Wahyudianingsih; Unang Supratman; Ronny Lesmana
Majalah Kedokteran Bandung Vol 54, No 1 (2022)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v54n1.2479

The high incidence of breast cancer cases in the world requires the use of applicative methods. The 7,12-dimethylbenz(a)anthracene (DMBA) induced breast cancer animal model is a widely used chemical-induced animal models for research on breast cancer. However, the molecular mechanism related to DMBA induction remains unclear. Good understanding on DMBA-induced animal models is crucial for studies related to future breast cancer treatments as animal models will provide a deeper understanding of anticancer medication, specifically those aimed for treating breast cancer. The aim of this study was to develop an DMBA-induced animal model for breast cancer. This study used female Wistar rats injected subcutaneously with DMBA as a carcinogen-induced agent (20 mg/kg) to induce tumor. Rat tumors were then evaluated and breast appearance was observed weekly, starting from day 28th after DMBA injection. Breast cancer tissue was then sampled and stored at -80°C until it was used for western blot and histological study. This study indicated that DMBA induced cancer in female Wistar rat’s breasts, and cytoplastic cells and lung metastatic was identified macroscopically and histopathologically. The metabolic sign was observed in the lung and breast sections. Interestingly, the DMBA induction in this study does not only induce organ cancers but also induces estrogen receptors and stimulates signaling of estrogen receptors α (ERα), ERβ, and Akt.

Effect of Dietary Sodium on α, β, and γ Epithelial Sodium Channel (ENaC) Gene Expression in Kidney Tubules of Wistar Rats Ronny Lesmana; Genta Syaifrin Laudza; Trianing Tyas Kusuma; Hanna Goenawan; Yulia Sofiatin; Yuni Susanti Pratiwi; Nova Sylviana; Setiawan Setiawan
Majalah Kedokteran Bandung Vol 51, No 4 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Hypertension is a condition of persistently high blood pressure. It is currently a big health issue as its prevalence is high in Indonesia and its complications are numerous and deadly. Salt intake is one of the modifiable factors of hypertension. According to a study by Indonesian Ministry of Health, salt consumption in Indonesia is almost two times greater than the recommended salt diet from WHO. Sodium reabsorption in kidney plays a role in regulating blood pressure. Epithelial sodium channel (ENaC) is one of the structures that function in sodium reabsorption in kidney tubules. This study was conducted at the Central Laboratory and Animal Laboratory of the Faculty of Medicine Universitas Padjadjaran from June to Desember 2018. The aim of this study was to analyze the effect of high sodium diet on the expression of ENaC gene in kidney tubules of rats. Twelve Rattus norvegicus wistar rats were divided into two groups of control and treatment. Treatment group was given daily 2 mL NaCl solution treatment using gavage for 8 weeks. The expression of ENaC α, β, and γ was obtained by running tissue samples from kidney cortex and medulla in polymerase chain reaction and electrophoresis. The result showed that there was an insignificant decrease in ENaC α, β, and γ gene expressions in both kidney cortex and medulla of the treatment group when compared to control groupThis study concludes that ENaC gene expression is not significantly affected by high sodium diet.Pengaruh Diet Tinggi Natrium terhadap Perubahan Ekspresi Gen Epithelial Sodium Channel (ENaC) pada Tubulus Ginjal TikusHipertensi merupakan kondisi tekanan darah tinggi dalam waktu lama. Prevalensi dan komplikasi hipertensi menyebabkan hipertensi menjadi isu kesehatan yang cukup besar. Jumlah asupan garam merupakan faktor hipertensi yang dapat dimodifikasi. Berdasar studi Kementrian Kesehatan Indonesia, konsumsi garam di Indonesia rerata dua kali lipat lebih banyak daripada rekomendasi WHO. Reabsorpsi natrium pada ginjal berperan penting pada regulasi tekanan darah. Fungsi ini diperankan oleh epithelial sodium channel (ENaC) yang berfungsi untuk reabsorpsi natrium. Penelitian ini dilakukan di Laboratorium Sentral dan Laboratorium Hewan Fakultas Kedokteran pada Juni–Desember 2018. Tujuan penelitian ini menganalisis efek diet tinggi natrium pada ekspresi gen dari ENaC di tubulus ginjal pada tikus. Duabelas ekor tikus Rattus norvegicus dibagi menjadi dua kelompok, yaitu kontrol dan perlakuan. Kelompok perlakuan diberikan 2 mL larutan NaCl setiap hari selama 8 minggu. Larutan diberikan melalui paksa (gavage). Ekspresi gen ENaC α, β, γ dari korteks dan medula ginjal diamplifikasi dengan PCR dan dideteksi dengan elektroforesis. Pita protein dari gel elektroforesis dinilai intensitasnya dengan software ImageJ. Hasil dari elektroforesis menunjukkan penurunan ekspresi gen ENaC α, β, γ di korteks dan medulla pada kelompok perlakuan dibanding dengan kontrol. Studi ini menyimpulkan ekspresi gen ENaC pada korteks dan medulla ginjal tidak signifikan dipengaruhi oleh diet tinggi natrium.

Effect of Exercise Duration on Klotho and GluN2B Gene Expressions in Hippocampus of Wistar Rats with Moderate Intensity Exercise Hanna Goenawan; Bela Ita Karina; Titing Nurhayati; Julia Windi Gunadi; Setiawan Setiawan; Ronny Lesmana
Majalah Kedokteran Bandung Vol 52, No 3 (2020)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v52n3.2026

Exercise could delay the degeneration process by increasing Klotho, which is an endogen antioxidant. Klotho protects against oxidative damages, regulates hippocampal synaptic plasticity, and enhances GluN2B expression. This study aimed to analyze the effect of 14-day and 8-week moderate-intensity exercise on Klotho and GluN2B mRNA expressions in rat hippocampus. This study was an animal experimental study using Wistar rats that was performed from June to September 2019 at the Central Laboratory, Universitas Padjadjaran, Indonesia. Rats were divided into 4 groups: control (14-day control and 8-week control) and exercise group (14-day exercise and 8-week exercise). The exercise groups ran at a moderate intensity (20m/min) for 30min/day, 5x/week. Hippocampus was then subjected to klotho and GluN2B gene expression analysis. The exercise groups showed a higher Klotho expression, both in 14 days (0.844±0.124 vs. 1.057±0.013) and 8 weeks (0.897±0.072 vs. 1.380±0.168) although statistically insignificant (p=0.24). No difference in GluN2B gene expression was observed between control and exercise groups on 14 days (1±0.09 vs. 1.22±0.09) and 8 weeks (1.24±0.03 vs. 1.38±0.05; p=0.11). In conclusion, 14-day and 8-week moderate-intensity exercise did not alter Klotho and GluN2B expressions in rat hippocampus. Pengaruh Durasi Olahraga terhadap Perubahan Ekspresi Gen Klotho dan GluN2B di Hipokampus Tikus Galur Wistar yang Mendapatkan Perlakuan Lari Intensitas SedangOlahraga dapat menghambat proses degenerasi saraf dengan meningkatkan salah satu antioksidan endogen, yaitu Klotho. Klotho dapat melindungi sel neuron dari kerusakan oksidatif dan meningkatkan sinaptik plasticity pada hipokampus. Penelitian ini bertujuan untuk melihat pengaruh olahraga lari pada hewan coba pada 14 hari dan 8 minggu terhadap perubahan ekspresi gen Klotho and GluN2B di hipokampus. Penelitian ini merupakan penelitian eksperimental dilaksanakan pada bulan Juni hingga September 2019 di Laboratorium Sentral Universitas Padjadjaran. Penelitian ini menggunakan tikus jantan galur wistar. Tikus dibagi menjadi 4 kelompok perlakuan dan kontrol. Olahraga dilakukan dengan treadmill tikus, kecepatan 20m/min, 30 menit perhari selama 5 kali perminggu. Kemudian, mRNA otak digunakan untuk PCR dengan menggunakan primer klotho dan GluN2B. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan yang bermakna (p=0.24) pada ekspresi gen Klotho pada 14 hari (0.844±0.124 vs 1.057±0.013) dan 8 minggu (0.897±0.072 vs 1.380±0.168) antara kelompok kontrol dan kelompok lari. Ekspresi gen dari GluN2B juga tidak menunjukkan perbedaan yang signifikan (p=0.11) pada 14 hari (1±0.09 vs 1.22±0.09) dan 8 minggu (1.24±0.03 vs 1.38 ± 0.05). Simpulan, bahwa 8 minggu olahraga tidak meningkatan ekspresi Klotho dan GluN2B yang signifikan

Co-Authors . SETIAWAN . Susianti Abd. Rasyid Syamsuri Abdul Hadi Hassan Ace Tatang Hidayat Achadiyani Agus Muji Santoso Agus Syahrani Agus Syahrani Ahmad Kurniawan Ahmad Kurniawan Ahmad Ramdan Ajeng Diantini Aldo Hartono Aldo, Mochammad Alia Bihrajihant Raya Alia Bihrajihant Raya Ambrosius Purba Ambrosius Purba Anang Wahid M.Diah Anas Subarnas Anas Subarnas Andrew Citrawan Anggraeni Dyah S Anggraeni Dyah Sulistiowati Anissa Anissa Anissa, Anissa Annisa F. Dewi Arief Budiono Arief Cahyanto Arief S. Kartasasmita Arifin Dwi Saputro Arto Yuwono Soeroto Astrid Feinisa Khairani Astrid, Stephanie Aulina, Datin Zahira Aziis Mardanarian Rosdianto Aziiz Mardanarian Rosdianto Aziiz Mardanarian Rosdianto Aziiz Rosdianto Azis Mardanarian Azmi, Mohamad Nurul Bachtiar, Willy Bahjah, Firda Hanan Baniasih, Akbar I. Baniasih, Akbar I. Bashari, Muhammad H. Bela Ita Karina Daba, Mariel Dani Sujana Darmayanti, Meita Decky Gunawan, Decky Dewi, Annisa F. Diana K Jasaputra Diana Krisanti Jasaputra Diana Krisanti Jasaputra Diandra Syifa Dimpuulina Erna Mariati Dwi Putri Larasati Edi Sukmana Edi Sukmana Edward J Hadi Endang Sutedja Endang Sutedja Enny Rohmawaty Euis Julaeha Evi Rinata Fajar Ari Widyatmoko Fani Rahma Yenita Fathurachman Fathurachman Fatona Suraya, Fatona Febriana Kurniasari Ferdyan Efza Fernandez, Veronica Fifi Veronica Filza Yulina Ade Finda Meyditia Finda Meyditia Fitri Fadhilah Galuga Sinalusur Sari Genta Syaifrin Laudza Gilang Muhamad Nur Iqbal Goenadi, Julia Windi Gunadi, Julia Windi Gunawan, Hanna Hadi Hadi Hana Goenawan Hanna Goeanawan Hanna Goenawan Hanna Goenawan Hanna Goenawan Hanna Gunawan Hanom Husni Syam Hasbilla, Raihan Fathurrahman Hendra Gunawan Hendy Setiawan Hendy Setiawan Herlina, Lien Herry Herman Hilmi Al Wahid Huda, Fathul Huda, Fathul I Made Joni Ida Ayu Putu Sri Widnyani Ieva B. Akbar Ieva Baniasih Akbar Ika M. Silviana Imam Megantara Indahwati, Dwiwahju Dian Irma Erika Herawati Irma Erika Herawati Irma Melyani Puspitasari Irma Ruslina Defi Isa Mahendra Ivan Christian Channel Ivanna Valentina Iwan Setiawan Iwan Setiawan Iwan Setiawan Iwan Setiawan Jatnika, Danti Dja Jihan Mudrika Rahmi Julia W Gunadi Julia Windi Gunadi Julia Windi Gunadi Jutti Levita Kanakarajah, Sarmmila Kartika Hajar Kirana, Kartika Hajar Kastella, Faysal Keiichi Motoyama Khairinisa, Miski A. Krisnadi, Sofie Riyani Kusmawan, Steven Zerin Putra Lazuardhi Dwipa Leonardo Lubis M.T., Vita Madhani Pradiptha Nugroho Maharani, Julia Maimun Syukri, Maimun Mariel Daba Mas Rizky A.A. Syamsunarno MEGANTARA, SANDRA Meita Dhamayanti Muchtaridi Muchtaridi Mulyanaga, William Giovanni Mutakin Mutakin Mutiarahmi, Citra Nur Nabilah Muhamad Naini, Al Arofatus Nanang Wahyudin Nanang Wahyudin Nanny N. N. M. Soetedjo Nasrul Wathoni Norisca Aliza Putri Nova Sylviana Nova Sylviana Nuraini Yasmin Nuroh Najmi Nurul Fadhilah Oki Suwarsa Okta Wismandanu Oktorina, Lenny Pangestuti, Balqist Sriprobo Pardede, Akim Manaor Hara Penny Setyawati Martioso Permadi, Andieni Faqhira Permana, Noah Jefferson Permana, Shenny S.S Pinkan Al Shabrina Pitu Wulandari Prasetyo, Wibowo Budi Prayudi Santoso Putri Tessa Putri, Aufa A.D Qiantori, Adziqa Ammara Radhiyanti, Putri Teesa Rahdiyanti, Putri Teesa Ramadhanti, Julia Ramdani Hamzah Ray, Hamidie Ronald Daniel Rejeki, Purwo Sri Renaldi Prasetia Reni Farenia Reni Farenia rima siburian Riska A Safira Risna Agustina Risna Agustina Roro Wahyudianingsih Rosdianto, Aziis Mardanarian Rosdianto, Aziiz M. Rosdianto, Aziiz Mardanarian Rurini Retnowati Rusdin, Agus Ruswana Anwar Safriansyah, Wahyu Salam, Supriyanto Sandra Amalia Riyadi Santi Sofiyanti Sarmmila Kanakarajah Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Shelly Iskandar Silviana, Ika M. Simanungkalit, Jayne Mary Siti Baitul Mukarromah Siti Mukarromah Siti Nuriyatus Zahrah Slamet Imron Sofie R. Krisnadi Sri Adi Sumiwi Steffi Liem Sudigdo Adi Sudrajat, Ratu Z.A.P sugianli, Adhi Sukmayani, Jasmine Y. Sunarjati Soedigdoadi Sunarjati Sudigdo Adi Sunarjati Sudigdo Adi Sunarjati Sudigdoadi Sunaryati S. Adi Supriatno Salam Susanti Husen Susianti Susianti Susianti Susianti Susianti, Susianti Susiarno, Hadi Tarawan, Vita M. Tarawan, Vita M. Teresa Lucretia, Teresa Teti Madiadipoera TIANA MILANDA Tina Rostinawati Titing Nurhayati Tjahjono D. Gondhowiardjo Tri Mayanti Trianing Tyas Kusuma Tyagita Hartady Unang Supratman Vita M. Tarawan Vita Muniarti Tarawan Vita Murniati Tarawan Vita Murniati Tarawan Vita Murniati Tarawan Lubis Vita Mutia Tarawan Wahyudianigsih, Roro Wahyuni, Indah S. Willy Bachtiar Yanuar Dwi Prastyo Yasmin, Nuraini Yenny Noor Yudi Feriandi Yulia Sofiatin Yumilia, Hoo Yunas, Aisya Astri Yuni Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi, Yuni Susanti Yusuf, Muchamad R.A Zaini, Lia M. Zakiyah, Neily Zulhendri, Felix

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