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Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Bidara Ziziphus Spina-Christi Dan Uji Aktivitas Antibakteri Terhadap Bakteri Staphylococcus Aureus Cut Dian Mala Luthfia; Dikki Miswanda; Haris Munandar Nasution; Minda Sari Lubis
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 1 (2024): Januari : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i1.168

Abstract

Nanotechnology is currently growing rapidly due to its broad application in science and technology. Nanotechnology itself is a science based on nanoparticles, so a more environmentally friendly method is being developed, namely the "green synthesis" method using plant extracts as a bioreductor. Silver nanoparticles have been synthesized using extracts of bidara leaf as a reducing agent. The objective of this research was was to determine the synthesis of silver nanoparticles using bioreductors and their application as antibacterial agents using bidara leaf extract against Staphylococcus aureus bacteria. This research used the green synthesis method to produce silver nanoparticles. The bidara leaf extract used was divided into several concentrations, namely 1 mM, 2 mM, 3 mM and 4 mM. To characterize silver nanoparticles, UV-Vis and PSA spectrophotometers were used. Furthermore, the Kirby-Bauer method was used to test the antibacterial properties of silver nanoparticles against Staphylococcus aureus bacteria. The results of research that has been done show that the formation of silver nanoparticles is characterized by the emergence of maximum absorbance peaks in the wavelength range of 400-450 nm, while the PSA results at concentrations of 3 and 4µm showed nanoparticle sizes of 185 nm. Silver nanoparticles of bidara leaf extract with various concentrations of 1 mM, 2 mM, 3 mM and 4 mM have antibacterial activity that can inhibit the growth of Staphylococcus aureus bacteria with an average inhibition zone diameter of 13.0617 mm, 11.7333 mm, 9.05 mm and 13.19 mm. This shows that silver nanoparticles with bidara leaf extract have antibacterial properties.
Uji Aktivitas Antibakteri Sabun Transparan Putik Saffron (Crocus sativus L) terhadap Bakteri Staphylococcus aureus Emmy Wiriandini; Gabena Indrayani Dalimunthe; Minda Sari Lubis; Haris Munandar Nasution
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 2 (2024): March : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i2.343

Abstract

Transparent soap is a variation of solid bar soap that not only functions to clean dirt but also has a scrubbing function. Transparent solid soap is one of the soap innovations that has become an attractive soap. Saffron also has antibacterial properties due to the presence of flavonoids, tannins and saponins. Flavonoids are compounds that can have anti-inflammatory, anticancer and antioxidant effects, because they can inhibit the formation of free radicals. It has been used traditionally to treat various ailments including depression, cardiovascular disease, menstrual disorders, asthma, insomnia, digestive ailments, bone pain and several other ailments. Saffron pistil juice is made by dissolving in CO₂-free distilled water. Phytochemical screening was carried out on saffron pistil extract. Saffron pistil juice is made in various soap formulas, namely formula I, formula II and formula III. The positive control used was chloramphenicol antibiotic and the negative control was DMSO. There were several tests carried out on Saffron pistil extract in addition to phytochemical screening, namely antibacterial testing on transparent soap with Saffron pistil extract as a soap additive. The results of phytochemical screening showed that the pistil of saffron (Crocus sativus L) contained secondary metabolites, namely: flavonoids, alkaloids, tannins, and saponins. And for the results of the antibacterial activity research also showed that the Saffron pistil extract against transparent soap which can be used as a soap additive, the antibacterial results had a strong inhibition at the average value of formula III which was 20.5 and formula II was 18.4 mm and formula I 16.1 mm, against Staphylococcus aureus bacteria.
Produksi Protein Sel Tunggal dari Kultur Saccharomyces cerevisiae dengan Medium Limbah Kulit Nanas (Ananas comosus L. MERR) Krisna Juniharta Napitupulu; Yayuk Putri Rahayu; Haris Munandar Nasution; Minda Sari Lubis
OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 2 (2024): March : OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/obat.v2i2.346

Abstract

Single cell protein is the term used for peoteins derived from microbes such as fungi. Pineapple waste is one of the alternative media that has easiest source of carbohydrates to obtain, so it has the potential for the growth of Saccharomyces cerevisiae. The purpose of this study was to determine wether pineapple waste could produce single cell protein production by adding nutrients to the fermentation medium. The research used in this research is experimental research. To determine the relationship between the independent variable and the dependent variable. Where the independent variables are the addition of nutrients and fermentation time on days 0,2,4,6 to see the dependant variable, namely the analysis of protein content, dry weight analysis of cells, analysis of glucose levels, pH and temperature analysis. The results of this study showed that the highest protein content was obtained in H4 fermentation by MFKN2, namely 0,43%, cell dry weight 0,851 grams, glucose content 1,3402%, ph 4,5, temperature 27,1 ⁰C. Meanwile, MFKN1 showed the highest protein content in H6 fermentation, namely 0,38%, cell dry weight 0,817 grams, glucose content 1,3397%, pH 4, temperature 26,2 ⁰C. The yield of glucose and pH tended to decrease as the fermentation process progressed until the last day of fermentation. The results of the temperature analysis tend to be stable as the fermentation process progresses until the last day of fermentation. The conclusion of this study is that pineapple waste can produced single cell protein from Saccharomyces cerevisiae culture and has different protein production with the addition of nutrients in the fermentation medium.
Drug Formulasi paper soap ekstrak etanol dedak padi (Oryza sativa L.) dan uji aktivitas antibakteri terhadap bakteri Escherichia coli dan Staphylococcus aureus Zannah , Muhamad; Dalimunthe , Gabena Indrayani; Lubis , Minda Sari; Yuniarti, Rafita
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.229

Abstract

Paper soap is soap in the form of thin sheets resembling paper. Rice bran from rice milling waste contains antibacterial compounds. The aim of this research was to formulate paper soap from rice bran ethanol extract and to determine its antibacterial activity. This research is a type of experimental research carried out in a laboratory. Includes steps such as taking and identifying samples, preparing and making ethanol extract of rice bran (Oryza sativa L.), and testing antibacterial activity against Escherichia coli using the Disc Disc method, where the inhibition zone of the ethanol extract of rice bran and the paper soap preparation is containing rice bran extract (Oryza sativa L.) was observed and measured as a result of the antibacterial activity test. The results of this research show that the ethanol extract of rice bran contains chemical compounds, namely alkaloids, flavonoids, saponins, tannins, triterpenoids/steroids and glycosides. Rice bran ethanol extract has characteristics in accordance with the 1989 MMI requirements. The results of determining the antibacterial activity of rice bran ethanol extract produce an inhibition zone against Escherechia coli bacteria ranging from 5mm - 12.5mm and Staphylococcus aureus ranging from 5.5 mm-16.67 mm. The results of the paper soap evaluation test, organoleptic examination, thickness examination, weight uniformity test, stability test, foam power test met the requirements and in the irritation tests F0, F1, F2 and F3 did not irritate the skin, in the hedonic test the most preferred formula was F1. The results of determining the antibacterial activity of paper soap produced an inhibition zone for Escherechia coli bacteria ranging from 8.16mm - 12.5mm and Staphylococcus aureus ranging from 15mm-14.83mm. From the research results it can be concluded that rice bran ethanol extract can be formulated into paper soap and has antibacterial activity.
Formulasi dan uji aktivitas antibakteri sediaan salep daging daun lidah buaya (Aloe vera L.) terhadap bakteri Staphylococcus aureus pada penyembuhan luka bernanah Dena, Mutia; Dalimunthe, Gabena Indrayani; Lubis, Minda Sari; Rahayu, Yayuk Putri
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.388

Abstract

Staphylococcus aureus is one of the bacteria that can cause infectious diseases of the skin, one of which is purulent wound disease. Aloe vera (Aloe vera L.) belongs to the family Asphodelaceae, which is a plant known to have antibacterial compounds. The study was conducted experimentally. The independent variable is the concentration of aloe vera leaf flesh, 20%, 30% and 40%. The concentration of aloe leaf meat ointment is 20%, 30%, and 40%. The dependent variables are the antibacterial activity of aloe vera leaf meat ointment preparations against staphylococcus aureus bacteria. The results of the antibacterial Zone of Inhibition (ZOI) value of aloe vera leaf meat against staphylococcus aureus of 8.1 mm (20% concentration); 8.5 mm (concentration 30%); and 10.5 mm (concentration 40%). Antibacterial ZOI value of aloe leaf meat ointment against staphylococcus aureus by 10.6 mm (F1 20%), 11.5 mm (F2 30%), and 12.5 mm (F3 40%). The test results on purulent wounds in rat test animals get moderate healing scores on F1, F2, F3 and K + (gentamicin sulfate 0.1%) strong, so it can be said that aloe vera leaf meat ointment preparations can inhibit antibacterial growth in purulent wound healing even with a moderate category compared to Gentamicin ointment 0.1% with a strong (sensitive) category.  
Formulasi sediaan pasta gigi herbal ekstrak etanol bunga telang (Clitoria ternatea L.) dan uji aktivitas antibakteri terhadap bakteri Streptococcus mutans Rati , Yuliandini; Dalimunthe , Gabena Indrayani; Lubis, Minda Sari; Nasution , Haris Munandar
Journal of Pharmaceutical and Sciences JPS Volume 7 Nomor 4 (2024)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v7i4.479

Abstract

Dental and oral disease is a disease experienced by almost half of the world's population (3.58 billion people). Gum (periodontal) disease is the 11th most common disease in the world. One of the dental and oral diseases that has a fairly high prevalence in Indonesia is dental caries. The aim of this research was to evaluate the physical quality of toothpaste preparations with various concentrations (10%, 30% and 50%) and to determine the antibacterial activity of ethanol extract toothpaste preparations from butterfly pea flowers (Clitoria ternatea L.) against the growth of Streptococcus mutans bacteria.The stages of this research included processing plant materials, making ethanol extract of butterfly pea with ethanol 96% solvent, characterization examination, screening of phytochemicals, making herbal toothpaste preparations of butterfly pea extract, preparation evaluation, and testing the antibacterial activity of butterfly pea toothpaste against Streptococcus mutans using the well-diffusion method. Based on the research results, it can be seen that the antibacterial activity of the formula with the greatest zones of bacterial inhibition is F1 and F2, namely 21.85 mm and 21.15 mm with very strong inhibition criteria. The conclusion of this study is that herbal toothpaste with butterfly pea extract can prevent and treat dental caries caused by Streptococcus mutans bacteria.
Pembuatan lip balm nanoekstrak umbi bit (Beta vulgaris L.) dan bunga rosella (Hibiscus sabdariffa L.) sebagai pelembab alami Jannah, Miftahul; Dalimunthe , Gabena Indrayani; Lubis , Minda Sari; Rani , Zulmai
Journal of Pharmaceutical and Sciences JPS Volume 7 Nomor 4 (2024)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v7i4.480

Abstract

One of the most sensitive parts of the outer skin on the face is the lips, as they are prone to extreme weather conditions such as heat and cold, which can cause dryness and chapping. To address this condition, it is essential to use a lip balm. One of the plants used as an active ingredient in lip balm formulations is beetroot and rosella flower. Nanoparticle-based cosmetics have advantages over micro-scale cosmetics. The objective of this research was to determine whether nano extracts of beetroot and rosella flower can be formulated into a lip balm preparation, whether beetroot and rosella flower extracts meet the characteristic requirements as nano extracts, and whether lip balm formulations from beetroot and rosella flower can be used as moisturizers. This research method is true experimental with a post-test control group design, where the results were observed after the treatment. The study uses samples of beetroot and rosella flowers. The stages of this research include sample preparation, characterization of simplicia, phytochemical screening, extraction and nano-extraction, lip balm formulation, physical quality evaluation, and moisture testing. The research results show that beetroot and rosella flower contain alkaloids, flavonoids, saponins, tannins, and anthocyanins. The beetroot and rosella flower extracts meet the characteristics of nanoextracts, with particle sizes of 261.64 nm for beetroot and 143.86 nm for rosella flowers. Beetroot and rosella flower formulated into a lip balm preparation can moisturize the lips, as evidenced by increased moisture and oil content on the lip skin before and after the preparation.
Formulasi sediaan pasta daging lidah buaya (Aloe vera (L.)Burm.f.) dan uji aktivitas antibakteri terhadap bakteri Streptococcus mutans Meutia, Cut; Dalimunthe , Gabena Indrayani; Lubis , Minda Sari; Yuniarti , Rafita
Journal of Pharmaceutical and Sciences JPS Volume 7 Nomor 4 (2024)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v7i4.524

Abstract

Introduction: Aloe vera (Aloe vera (L.) Burm.f.) is a plant that contains 95% water. The rest is in the form of other active ingredients (essential oils, amino acids, minerals, vitamins, enzymes, and glycoproteins). The type of aloe vera used to make herbal aloe vera is the Barbadensis type, which contains substances for human needs, such as Vitamins A, B1, B2, B6, B12, Vitamin E, and Vitamin C. This plant can also cure diabetes, heart disease, and other diseases.  Aloe vera can be formulated into toothpaste preparations because it can improve hygiene and reduce plaque in the mouth. Objective: This study aimed to make a new formulation of (aloe vera (L.) Burm.f.) as a toothpaste preparation and evaluate its antibacterial activity against Streptococcus mutans bacteria. Methods: This study uses an experimental method. In this study, aloe vera was formulated as toothpaste. The physical evaluation carried out included tests for water content, total ash content, water-soluble extract content, ethanol-soluble extract content, acid-insoluble extract content, organoleptic test, oh test, homogeneity test, spreadability test, adhesion test, test viscosity, and foam formation test. Results: Based on the research results, it can be seen that aloe vera flesh has antibacterial activity against Streptococcus mutans bacteria. Conclusion: This research concludes to make a new formulation of (aloe vera (L.) Burm.f.) as a toothpaste preparation and evaluate its antibacterial activity against Streptococcus mutans bacteria.
Formulasi sediaan pelembab bibir mengandung ekstrak kulit buah pisang raja (Musa paradisiaca L) Siregar , Runisya Maugin Utami; Yuniarti, Rafita; Lubis, Minda Sari; Nasution, Muhammad Amin
Journal of Pharmaceutical and Sciences JPS Volume 7 Nomor 4 (2024)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v7i4.674

Abstract

Lip balm is a care product that can keep your lips moist, which is a type of ointment used to treat dry and chapped lips. The benefit of using lip balm for lips is to regulate lip moisture. Lip balms are generally clear or colorless, although recently colored variants have also been created. To find out whether the extract of plantain peel (Musa paradisiaca L) can be formulated in the form of lip balm preparations. The purpose of this study was to determine whether lip balm preparations could be used as moisturizers. To find out lip balm preparations well made and of good physical quality. This research method is experimental. Collection of plants was carried out purposively, ie without comparison with other areas. The sample used was the skin of fresh ripe plantains obtained from the Simpang Limun market in Medan. This study included quality inspection preparations, moisture effectiveness test preparations, irritation tests on formulations and hedonic tests on variations in preparations made. The results of this study indicate that extracts of plantain peel (Musa paradisiaca L) with concentrations of 1%, 3%, 5%, 10%, and 15% can be formulated in the form of lip balm preparations. Formula 5 with a concentration of 15% showed the best lip balm preparation by fulfilling the requirements for physical quality, effectiveness and safety. With a preference value of 5 (like very much), has a melting point of 57'C and has a pH of 5.9 so it does not cause skin irritation and is stable at room temperature storage for 28 days. Lip balm preparations made using cocoa butter base are safe to use and meet the standards..
Formulasi masker wajah serbuk nano teh celup bekas dan aktivitas antibakteri terhadap Propionibacterium acnes dan Staphylococcus epidermidis Matondang , Fikriyah Hafni; Lubis, Minda Sari; Yuniarti , Rafita; Rani, Zulmai
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.685

Abstract

The increase in the cosmetic industry has caused a variety of facial mask products to circulate on the market, making it difficult for consumers to find one that suits their skin condition. Masks from well-known brands are easy to get, but some cause skin problems. Since ancient times, Indonesians have used plants as medicines and cosmetic ingredients for skin care. Tea can be processed into new active ingredients such as antioxidants, antibacterials, and anti-aging for cosmetics. Nanoparticle-based cosmetics are superior to micro-scale cosmetics. The objective of the research was to determine whether used tea bag powder meets the characteristic requirements as nanopowder, to determine whether used tea bag nanopowder in facial mask preparations meets the physical quality requirements, and to determine whether used tea bag nano powder facial mask preparations has antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis. This research method was true experimental with post test only control group design research design. The independent variables are tea bag powder and nanopowder and powder face mask formulation variations. The dependent variables are the characteristics of the simplicia, secondary metabolites, nanopowder characteristics, powder face mask formulation, and antibacterial activity test. The results showed that tea bag powder and nanopowder contained alkaloid, steroid, flavonoid, saponin, and tannin compounds. The inhibitory activity of the used tea bag nano powder mask on Propionibacterium acnes was 11.3 mm on Staphylococcus epidermidis, which was 11.2 mm. The tea bag powder used meets the characteristics of a nanopowder with a particle size of 684 nm. I used a tea bag nanopowder to prepare a face mask that meets the physical quality requirements.
Co-Authors A'dilah, Nur Adani, Bazlina Adela Octi Dwiyani Adjelie, Tiara Afifah, Puan Ibni Alfi Wahyudi Nasution Alma Dhita Shafira Amaliana, Amaliana Andjelie, Tiara Anggraini, Dea Puspa Anny Sartika Daulay Anugrah, Bayu Ariandi Arianti, Sellin Arina Aulia Fitri, Aulia Aulia, Meisyah Ayuningrum, Annisa Bambang Hermanto Chairina Milda Cindy Marlina Tambunan Cut Dian Mala Luthfia D Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D.Elysa Putri Mambang Debi Meilani Dena, Mutia Denni Bahari Dewa, Fasca Dewi, Sri Harti Dikki Miswanda Dinda Sari Utami Efendi, Tania Tiara Ellyza, Chella Emmy Wiriandini Fauziah Zain Fithri Pulungan, Ainil Fitri Febriani Fitri, Nurul Fitri, Raissa Gabena Indrayani Dalimunthe Haris Munandar Nasution Hasibuan , Fika Miah Sasmitha Hendraputra, Hardy Handoko Heru Adha Prayogo Husna, Miftahul Hutagalung , Septi Ratna Cempaka Hutagalung, Riris Br Ika Fitriani Indah Nasution Inna Myesha Junia Fitri Hayani Juwita, Sukma Karismawati Karlina Butar Butar Khoyrill Muttiin Kiki Rawitri Krisna Juniharta Napitupulu Lestari, Seri Lestari, Sri Indah Linda, Rosa Lisda Mayanti Lubis, Nadya Frestika Lubis, Nurazizah Lubis, Zulkifli Ismail M. Arya Wiguna Madani Sirait maghfirah, Suci Mambang, D Elysa Putri Matondang , Fikriyah Hafni Maulana Putri, Yasinta Meutia, Cut Meylisa Pratami Br Sinaga Miftahul Jannah Milwani Harahap Miranza, Nona Misna Rosalinda Hutabarat Mohd. Bilal Muhammad Amin Nasution Muhammad Tegar Tri Rizky Nadia Salsabilla Nasution , Muhammad Amin Nasution, M Pandapotan Nasution, M. Pandapotan Nasution, Vevi Sarah Nia Novranda Pertiwi Ningtias, Anggitha Novi Yuliandari Novita Sari, Helsa Novriani Putri Santia Nur Asia, Nur Nuratika, Eli Nurfadilla, Kiki Nurul Salsa Abya Ritonga Nurwijayanti Pulungan, Anili Fithri Purba , Intan Sabila Putri Purba, Juvantri Fablo Puteri, Cut Intan Annisa Putri , Adilla Rafita Yuliarti Rafita Yuniarti Rafita Yuniarti Rafita Yuniarti Rahma Maulidia Fitri Rahmi, Sofia Ramadhan , Yusril Rambe, Sitiapsah Rapita Yuniarti Rati , Yuliandini Rati Satri Situmorang Rezky , Deswita Ina Riani, Nur Aslin Ridwan Taher Lubis Ridwanto Ridwanto Ritonga , Nur Sakina Ritonga , Septi Ani Ritonga, Oktavia Rohmah, Roihanah Rosalinda Mahdalena Sinaga Rosalinda Mahdalena Sinaga Safitri , Nurhaliza Sagita Marina Simatupang Sandika, M Teguh Sari, Syilvi Rinda Savika, Anggraini Selvicahyani, Selvicahyani Shinta Mida Ariani Harahap Sihotang, Laura Indah Restu silalahi, zahra salsabila Sinaga, Rosalinda Mahdalena Sindi, Sindi Siregar , Misbah Siregar , Runisya Maugin Utami Siregar, Hikmatussabaria Siregar, Rina Paramitha Siti Aisyah Jamil Siti Anisa Sitinjak, Iyustri Ririn Slamet Indarjo Sri Harti Sri Mulia Ningsih Siregar Sri Murni Sri Ria Ranti Sri Wahyuni Sulwiyatul K Sani Supiyani, Supiyani Surendro, Yasmeen Nazhifah Amani Syafa Nadira Ashiilah Syafira, Nona Syafirna, Nada Tia Nazilla Titin Agustina Trisha Wulan Erja Ummu Safura Sirait Vera Kristiana Wan Dian Safina Widya, Rizka Wilda Septia Winda Aulia Yadi, Hervi Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Yuliana Yuni Sartika Yuniarti , Rafita Yuniarti, Rafita Yuniarti, Rapita Zahara, Fitria Siti Zahira Ahmad, Madani Zannah , Muhamad Ziza Putri Aisyia Fauzi Zulhij, Fizrya Zulmai Rani