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Journal : journal of Basic Medical Veterinary

HISTOPATHOLOGICAL APPEARANCE OF THYMUS ON BROILER UNDER CHRONIC HEAT STRESS Andriani, Ratna; Arimbi, Arimbi; Rahardjo, Dadik; Plumeriastuti, Hani; Legowo, Djoko; Hestianah, Eka Pramyrtha
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19987

Abstract

The aim of this research was to know histopathological change of broiler's thymus which exposed by chronic heat stress. The results revealed that there were negative impact on immune organ (thymus). Broiler that exposed to chronic heat stress showed significantly increased number of heterophil and apoptosis histiocytes and cell depletion. There were 20 broilers that divided into 2 groups, each group consist of 10 broiler. Control group (P0) reared at temperature 24-28oC and humidity 40-55% and Heat Stress group (P1) reared at temperature 36-40oC on the litter housing system at Experimental Animals Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. Before the treatments, broiler had been reared from day-old-chicks to 21-day-old, then continued with heat stress condition from day 22 to 42. Thymus was collected on day 42 to examine histopathological change of thymus and analyzed using Mann-Whitney Test. The results showed significant difference (P<0.05) between Control group and HS (Heat Stress) group by increased number of heterophil, apoptosis histiocytes and cell depletion.
EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Nursadida, Milla; Plumeriastuti, Hani; Dhamayanti, Yeni; Widjiati, Widjiati; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

Abstract

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Jeanita, Winny; Meles, Dewa Ketut; WIdjiati, Widjiati; Hamid, Iwan Sahrial; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

Abstract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.
GAMBARAN PATOLOGI HEPAR IKAN LELE DUMBO (Clarias gariepinus) YANG DIPAPAR LOGAM TIMBAL NITRAT Pb(NO3)2 Dellavia, Yolanda; Widiyatno, Thomas Valentinus; Setiawan, Boedi; Arimbi, Arimbi; Poernomo, Bambang; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21092

Abstract

This research aimed to know the changed damage of liver African catfish (Clarias gariepinus) were exposed by lead nitrat. This study used twenty four of African catfish (Clarias gariepinus) with average weight of 20-25 grams, size 10-12 cm, age ± two months. This research was designed by a completely randomized design (CRD). All member of population of the African catfish were divided into six groups, consist of six repetitions each, namely P1, P2, P3, and P0 as a negative control respectively.  P1 was given with dosage of lead nitrat 7,26 mg/liter, P2 was given with dosage of lead nitrat 14,53 mg/liter, and P3 was given with dosage of lead nitrat 29,06 mg/liter. According to the macroscopic observed, show that liver became swollen and pale.  The histopathological features of hepar were examined under light microscope in 400 times magnification. Scoring method were using Bernet Scoring Method to examined the presence of degeneretion, congesti, necrotic, and infiltration of leukocyte. Then, Kruskal-Wallis test through with Mann-Whitney test of statistical analysis. The statistical analysis showed the median number of P0 (0,05), P1 (0,45), P2 (1,10), and P3 (1,40) respectively. From the result can be concluded lead exposure with dose 29,06 mg/liter severe which was heavily histopatological in hepatocytes cell of african catfish (Clarias gariepinus) liver.
The Efficacy of Putrimalu Root Extract (Mimosa pudica) Against Mortality and Histopathological of Rat Muscles Injected Naja sputatrix Venom Rendragraha, Briantono Willy; Legowo, Djoko; Kuncorojakti, Suryo; Chusniati, Sri; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36486

Abstract

The purpose of this experiment was to know the effect of Mimosa pudica root extract (normal water extraction) on mortality and muscle histopatological in experimental animal (Rattus norvegicus) which injected with LD50 of Naja sputatrix venom. Fresh Mimosa pudica root dried without sunshine and ground into coarse powder with steamroller (mash 3mm). Plant extract were prepared by stirring 4 g of the powder into 200 ml of water for 3 hours at room temperature and filtered with muslin cloth before freezdried. 30 rat divided into five groups, P0(C+), P1(C-) P2, P3, and P4. P0 (positive control group) injected by saline intra muscular and given aquadest peroral after five minutes, P1 (negative control) injected with Naja sputatrix venom and given aquadest peroral after five minutes. P2, P3, and P4 injected by Naja sputatrix venom and given Mimosa pudica extract with 250mg/KgBW, 500mg/KgBW, and 1000mg/KgBW doses. All of the experimental animal observed about six hours and than euthanized, musculus gluteus maximus (location of injection) was taken for histopatological examination. In this experiment observed that the normal water extraction of mimosa pudica can't reduce mortality of experimental animals but it can decrease muscle damage in 1000mg/KgBW dose.
Co-Authors A. Muh. Ashabul Kahfi P. A. Muh. Raehan Abdul Rahman Abdul Rahman Abdul Rahman Ahmad Akbar Ismail Ahmad Dani Ahmad, Susiana Ahyani, Khilda Rahma Aliyah, Nazwa Himatul Andi Atssam Mappanyukki Andi Wirawan Risal Andriani, Ratna Anggraeni M, Andi Sri Dewi Anggraeni. M, Andi Sri Dewi Arfanda, Poppy Elisano Arifuddin Usman Arifuddin Usman Arifuddin Usman Arsal, Ulfah Widyastuti Asfar, A.M.Irfan Taufan Asfar, Andi Muhamad Ikbal Awaluddin Awaluddin Badaru, Benny Bafirman HB Bambang Poernomo Bayu Budi Prakoso Boedi Setiawan Carlos Carlos Chris Jhon Simanungkalit Chusniati, Sri Dadik Rahardjo, Dadik Darsono, Roesno Darul Husnul Dayanti, Efin Windi Dellavia, Yolanda Dian Ayu Permatasari Eka Pramyrtha Hestianah, Eka Pramyrtha Eri Barlian Etno Setyagraha Fahrizal Fahrizal Failasufa, Hayyu Febriyanda, Andini Fitrah Nur Athirah Fitriani Fitriani Fuad Fatkhurrohman, Fuad G, Sarifin G., Sarifin Haeril Hani Plumeriastuti Harelas, Genoveva Callista Hasmyati, Hasmyati Heryanto Nur Muhammad HIKMAD HAKIM Hudain, Muh. Adnan Ians Aprilo Ichsani Ichsani Ichsani, Ichsani Ihsan, Andi Ikhsan Ikhsan IMAM MUSTOFA Irfan Yunus Irvan Irvan Ishak Bachtiar Iwan Sahrial Hamid Jaya, Sonyman Jeanita, Winny Juhanis Juhanis Kuncorojakti, Suryo Legowo, Djoko Lestari, Tia Tri Luqman, Epy Muhammad M Imran Hasanuddin M, Andi Sri Dewi Anggraeni M. Rachmat Kasmad Machmudah, Machmudah Made Agus Wijaya Malik, Farid Giffari Mappaompo, M. Adam Mappaompo, M. Adam - Mardiana Mardiana Marjam Desma Rahadhini Meles, Dewa Ketut Milanda Prihastiwi Moch. Rizky Darmawan Muhamad Alfryan Muhamad Shandy Muhammad Akram Muhammad Kamal Muhammad Nur Muhammad Rhesa Muhammad Thohawi Elziyad Purnama Muhammad Zulfikar Muhammadong Mukrim, Harvina Muriyati Mutmainnah Mutmainnah Nadia Greciana Naufal Naufal Nining Widyah Kusnanik Nita Nita Novitasari, Seline Putri Nukhrawi Nawir Nukhrawi Nawir Nur Fadly Alamsyah Nurliani Nurliani Nurliani Nurliani Nursadida, Milla Oktaviana Bangun Paramita, Chici Ayu Poedji Hastutiek Poppy Elisano Arfanda Poppy Elisano Arfanda Pradana, Febryansah Gilang Aris Praja, Ratih Novita Prameswari, Febriana Hawa Pramudita, Tegar Pratama, Muh. Rama Puspodari, Puspodari Putera, Shidqi Hamdi Pratama Putri Sri Maharani Qibtiyah, Mariyal R. Rusli Rachamat Syarif Hidayatullah Rahmat Adi Jaya Ramli Ramli Rendragraha, Briantono Willy Retno Farhana Nurulita Retno Kusniati Rezkiyanti Mutmainna Abdullah Ricardo Valentino Latuheru Ririn Ririn Risna Y, Dyah Ayu Roro Riva Fani Rochmah Kurnijasanti Rofiatul Muntaha Ruslan Ruslan Rusli Rusli Rusli, Rusli ully Safaruddin Safaruddin Saharullah Saharullah Saharullah, Saharullah Sakinatina, Sintok Nabila Saleh, Muh. Syahrul Sanrebayu Sanrebayu Sanrebayu, Sanrebayu Saqinah, Putri Nurul Sarifin G Sarifin G Sarifin G, Sarifin G Sarifin Sarifin Sarifin, Sarifin Septirahellis Nur Afifah Siti Nur Azizah, Siti Nur Sri Mulyati Sri Pantja Madyawati, Sri Pantja Srimularahmah, Andi Sudirman Sudirman Sumaryanti Sumaryanti Suroto Suroto Surur, Andi Ainun Zulkiah Suwardi Suwardi Suwardi Suwardi Syahruddin Syahruddin Tafaawo Larosa TRI HARTITI Usman, Arifuddin Wahid, Wahyana Mujari Wahyana Mujari Wahid Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Widyastuti Arsal, Ulfah Wirawan R, Andi Yeni Dhamayanti Yudhana, Aditya Yunita, Maya Nurwartanti