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All Journal Jurnal Veteriner Media Kedokteran Hewan Aceh Journal of Animal Science Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology LOSARI: Jurnal Pengabdian Kepada Masyarakat Bima Abdi: Jurnal Pengabdian Masyarakat Jurnal Kedokteran Hewan Journal of Fisheries & Marine Jurnal Agro Veteriner
Rahmahani, Jola
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia 60115
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Social Sciences Transportation Veterinary Other

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Isolation and Identification of Salmonella Spp. on Broiler Meat at Banyuwangi and Blambangan Traditional Markets, Banyuwangi Regency Putri, Diana Novita; Rahmahani, Jola; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.219-224

Abstract

Salmonella spp. is one of the major causes of the world's foodborne disease that can spread through meats, eggs, or milk. Salmonella spp. is an enteropathogenic bacteria that can cause gastrointestinal infections. This study aimed to identify the contaminations of Salmonella spp. bacteria on the broiler meats at Banyuwangi and Blambangan traditional markets Banyuwangi district. The method of this study used 24 samples of broiler meat bought from Banyuwangi and Blambangan traditional markets in the Banyuwangi District. The samples were collected using the purposive sampling method. The results found that 5 out of 24 samples (21%) were tested positive for Salmonella spp. contamination and 19 were tested negative. This study was indicated that broiler meats at Banyuwangi and Blambangan traditional markets Banyuwangi district have not suitable with SNI (Indonesia's National Standard) 3924:2009 that requires at least 25 gram of fresh chicken meat to be free of or tested negative for Salmonella.
Characterization of Gene Coding Fusion Protein of Newcastle Disease Virus Infected in Native Chicken in Surabaya Kirana, Maha; Ernawati, Rahaju; Rahmahani, Jola; Rantam, Fedik Abdul
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.103-108

Abstract

This study aimed to discover the homology of nucleotide sequence, homology percentage, and those relations phylogenetic of protein Fusion (F) gene coding of Newcastle disease in domestic chicken (Gallus gallus domesticus) in Surabaya using some comparison isolate from GenBank. Samples were scoured of digestive organs from native chicken, that was collected from a traditional market in Wonokromo, Surabaya. Samples were tested using RT-PCR with primer forward and reverse with target 976bp, a positive sample which is continued with sequencing then homology and nucleotide analysis which is done and which is translated into amino acid. The result of homology chc/SBY/2018 sample has a similarity of 88% with references sequence, while with Lasota vaccine has a similarity of 87%, and the highest result of homology showed by the comparison with various isolates in Indonesia 90-95%. Translation results from nucleotide alignment into amino acid showed shifts in amino acid structure, which is amino acid shifts could be affected by many things like nutrition, wheater, environment, etc. The conclusion was chc/SBY/2018 sample has a quite high similarity with Indonesian isolates and undergoes mutation on nucleotide structure on amino acid and phylogenetic analysis. This study related to some isolates of vaccine and some isolates in Indonesia.
Retrospective Study of Feline Panleukopenia Risk Factors in Pet Cats in Madiun Baroroh, Diana Nova; Tyasningsih, Wiwiek; Praja, Ratih Novita; Rahmahani, Jola; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.114-119

Abstract

This study analyzed the association between risk factors and Feline panleukopenia at the Ichiyo Veterinary Clinic, Madiun using a retrospective method. A total of 2037 data used was secondary data obtained from cat medical and ambulatory reports from January 2018-March 2021. The risk factors analyzed included age, gender, vaccination status, comorbid diseases, housing system, number of cats, and season. Data was presented descriptively and analyzed using Chi-Square analysis. The results showed that the risk factors for Feline panleukopenia were cats aged ≤6 months, cats that were not vaccinated, and cats with comorbid diseases. It can be concluded that prevention of Feline panleukopenia can focus on age, vaccination and treatment of potential comorbid diseases in cats.
Comparison of Antigenicity Between Local and Massachusetts Strains of Infectious Bronchitis Virus using Indirect ELISA Test Rahmahani, Jola; Akbar, Daniswara Irwanza; Suwarno, Suwarno
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.28-33

Abstract

The aim of this study was to compare the antigenicity between the whole virus and protein S of both local strain I-147 and Massachusetts strain of Infectious bronchitis virus with indirect ELISA. We used 10 positive serums from post vaccinated chickens for further reaction with four groups of antigens in this study that are: P1) S protein (spike glycoprotein) antigen of local strain IB virus; P2) S protein (spike glycoprotein) antigen of Massachusetts strain IB virus; P3) whole virus antigen of local strain IB virus; P4) whole virus antigen of Massachusetts strain IB virus. The results of this research are Optical Density (OD) values of each treatment, then to be analyzed using One Way ANOVA and followed by Duncan Multiple Range Test 5%. The results show that there are differences between the antigenicity of the whole virus and S protein in both local strain and Massachusetts strain of IB virus. The whole virus antigen of both strains has a significant difference (p<0.05) with the S protein antigen in both strains. Specifically, the whole virus antigen of Massachusetts strain has the highest OD among the other groups.
Immunogenicity of Local and Massachusetts Strains Infectious Bronchitis Virus Rahmahani, Jola; Wudhu, R Zaksara Fero Ali Mutaqin; Suwarno, Suwarno; Tacharina, Martia Rani
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.98-102

Abstract

This study aimed to compare the immunogenicity between the whole virus and protein S of both local strain I-147 and Massachusetts strain of Infectious bronchitis virus with indirect ELISA. 24 healthy mice are divided into four groups with six mice in each group that are: P1) a group of mice that are vaccinated with S protein (spike glycoprotein) of local strain Infectious Bronchitis virus; P2) a group of mice that are vaccinated with S protein (spike glycoprotein) of Massachusetts strain Infectious Bronchitis virus; P3) a group of mice that are vaccinated with the whole virus of local strain Infectious Bronchitis virus; P4) a group of mice that are vaccinated with the whole virus of Massachusetts strain Infectious Bronchitis virus. The results of this research are Optical Density (OD) values of each treatment, then to be analyzed using One Way ANOVA and followed by Duncan Multiple Range Test 5% and T-test. The results show that there are differences in immunogenicity between the whole virus and S protein in both the local strain and Massachusetts strain of Infectious bronchitis virus. The OD value of the whole virus in the two strains has a significant difference from the OD value of the protein S antigen in both strains, both in the serum taken at week 2 and week 4 post-vaccination.
Serological Study of Newcastle Disease in Ducks (Anas javanicus) Slaughtered in East Surabaya Traditional Market Ardhanella, Sellianova; Damayanti, Ratna; Suwarno, Suwarno; Rantam, Fedik Abdul; Rachmawati, Kadek; Khairullah, Aswin Rafif; Rahmahani, Jola
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.131-137

Abstract

The purpose of this study was to determine the serological study of Newcastle Disease that infects slaughtered ducks (Anas javanicus) at the East Surabaya Traditional Market. This study was conducted from December 2018 to January 2019. A total of 122 samples were collected from four traditional markets in East Surabaya. The Haemagglutination Inhibition (HI) test was used to determine a positive result with an antibody titer of 4 or more. Samples for the HI test were collected from duck serum that had been treated with chicken red blood cells. The results showed 13 (10.65%) of the 122 samples were positive for ND.
Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method Rahmahani, Jola; Kirana, Maha; Rantam, Fedik Abdul; Suwarno, Suwarno
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.145-148

Abstract

This study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and extracted, then the Polymerase Chain Reaction (PCR) test was performed using Primers Hexon A (5'-CAARTTCAGRCAGACGGT-3') and Hexon B ( 5'-TAGTGATGMCGSGACATCAT-3' ) with 897 bp length. The results showed that the broiler chicken case sample from Banten was positively 100% infected by the IBH Virus.
Detection of Foot and Mouth Disease Virus in Cattle in Lamongan and Surabaya, Indonesia Using RT-PCR Method Dinana, Zayyin; Rantam, Fedik Abdul; Suwarno, Suwarno; Mustofa, Imam; Rahmahani, Jola; Kusnoto, Kusnoto
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.191-196

Abstract

Foot and mouth disease (FMD) virus was reported as an outbreak in Indonesia in April 2022 and belonged to serotype O/ME-SA/Ind-2001e is spread in the country. This study aimed to detect the causative agent based on clinical symptoms in cattle that have the vesicle in the mouth and hooves. A total of 25 samples were collected during August 2022 from Lamongan and Surabaya, Indonesia. FMD was identified in 58% (7/12) using reverse transcription polymerase chain reaction (RT-PCR), respectively. The samples were performed using universal primers with 328 bp length for primary diagnosis of FMD. These findings indicate that the spread of FMD viruses is highly contagious, so rapid and accurate diagnosis is needed as an effort to control and monitor FMD viruses.
Risk factors of pyometra in cat at several clinics in Kediri, East Java, Indonesia Rosyta, Pegy; Rimayanti Rimayanti; Budi Utomo; Jola Rahmahani; Yulianna Puspitasari; Wiwik Misaco Yuniarti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.115-119

Abstract

Pyometra is a reproductive disease that most often affects cats which is caused by bacterial infection of the uterus. This study aims to determine the risk factors that could influence the occurrence of pyometra in cats. The study was conducted at the Four Leg Pet Care, Asa Animal Clinic, and K-5 Animal Care in Kediri regency in December 2021 to February 2022. This retrospective cohort study took secondary data for the period 2020 to 2021 as research material and found 35 cats diagnosed with pyometra. The odds ratio was calculated to analyze the associations to some factors including age, body weight, use of hormonal contraception, frequency of administration of hormonal contraception, hormonal contraceptive drugs used, dose of hormonal contraception given.  Analysis of the relationship between risk factors for pyometra showed that the factors age, use of hormonal contraception, and frequency of giving hormonal contraceptives each have p values ​​of 2.17, 2.13, and 2.13, respectively. It could be concluded that age, use of hormonal contraception, and frequency of administration of hormonal contraception influenced or had an association with the risk of pyometra in cats.
Molecular Detection of FIPV among Imported Felines through Soekarno Hatta Airport, Indonesia Fitrawati, Fidyah; Suwarno; Yudaniayanti, Ira Sari; Rahmahani, Jola; Yunus, Muchammad; Soeharsono; Karo-karo, Desniwaty; Haeriah
Media Kedokteran Hewan Vol. 36 No. 1 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i1.2025.40-49

Abstract

One of the viruses that can cause disease in cats is feline coronavirus (FCoV). This virus is often divided into type I and type II. Type I is a highly pathogenic strain, feline infectious peritonitis virus (FIPV). Type II is a milder strain, feline enteric coronavirus (FECV). The FIPV variant is said to be a result of a mutation from FECV. FIP disease is responsible for 0.3%-1.4% of cat deaths in veterinary clinics. This study aims to determine if there is an FIPV in imported cats at Soekarno Hatta Airport, Indonesia. Samples were taken from 15 imported cats from Russia and Vietnam. These two countries were chosen based on previously unreported cases. The samples, consisting of blood and rectal swabs, were tested molecularly using RT-PCR. Four samples from rectal swabs showed positive results with a single band at 677 bp. Two positive samples, namely 123v and 682v, were further sequenced. The study results indicate that the FCoV virus can be found in asymptomatic imported cats. Further research is needed to better understand the mechanism causing genetic diversification of FCoV or FIPV and its impact on the pathogenesis of FIP. Furthermore, the application of FIP vaccines from other countries should be tested for compatibility with the FIPV strains present in Indonesia.
Co-Authors A'la, Rofiqul A. T. Soelih Estoepangestie Adikara, Tatang Santanu Agustono, Bodhi Akbar, Daniswara Irwanza Akhmad Taufiq Mukti Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Ali Agus Amal, Mohammad Noor Azmai Amelia, Ezanti Nur Annas Salleh Aprinda Ratna Lovela Ardhanella, Sellianova Arif Nur Muhammad Ansori Asri Rizky Asri Rizky Astria Novitasari Nidom Astuti, Elita Sriwidya Aswin, Ahmad Azahro, Aisyah Aziz, Githa Nurma Balqis Afifah Bambang Sektiari Lukiswanto Baroroh, Diana Nova Boedi Setiawan BUDI UTOMO Christian Marco Hadi Nugroho Chusniati, Sri Daruti Dinda Nindarwi Dewi, Nina Nurmalia Dhani Prakoso Didik Handijatno Didik Handijatno Dita Wisudyawati Diyantoro Diyantoro, Diyantoro Elisa Herina Dimariwu Erma Safitri Ernawati, Rahaju Ernawati, Rahayu Fachriza Tri Aprilia Fachrur Rozi Fadlilah, Shinta Levea Ni'matul Faisal Fikri Fedik Abdul Rantam Fidyah Fitrawati, Fidyah Gazali Salim Haeriah Hani Plumeriastuti Harijani, Nenny Helen Susilowati, Helen Herry Agoes Hermadi Hutahaean, Ryan David Pandapotan Imam Mustofa IMAM MUSTOFA Indah Rahmawati Ine Karni Ira Sari Yudaniayanti Irfani, Dyah Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Karo-karo, Desniwaty Khairullah, Aswin Rafif Khanand, Nadeem Kirana, Maha Kuncoro Puguh Santoso Kuncorojakti, Suryo Kuncorojati, Suryo Kurniasari, Pitaloka Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lucia Tri Suwanti, Lucia Tri Meles, Dewa Ketut Mohammad Noor Amal Azmai Mohammad Sofi’ul Anam Muchammad Yunus Muhammad Arief Muhammad Khaliim Jati Kusala Muhammad, Abdul Latif Mustofa Helmi Effendi Nidom, Chairul Anwar Nor Laela Ramadhaniyah Nurwartanti, Maya Nusdianto Triakoso Pasang, Gabriel Sampe Pinayungan, Probo Probo Warih Tatag Pradhita, Dewanggi Kristi Sasmi Praja, Ratih Novita Prestalia Dwi Rachmawati Putih, Nurvita Putri Desi Wulan Sari Putri, Diana Novita Rahaju Ernawati Rahaju Ernawati Rahmawati, Anlisia Rahmawati, Kadek Rahmi Sugihartuti Ramandinianto, Sancaka Chasyer Ratna Damayanti Reviany Vibrianita Nidom Rimayanti Rimayanti Rosyta, Pegy Rozi Sabdoningrum, Emy Koestanti Salleh, Annas Santanumurti, Muhammad Browijoyo Sari, Putri Desi Wulan Setyarina Indrasari Siti Komariyah Soeharsono Soeharsono Soeharsono Supriyadi Supriyadi Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Tacharina, Martia Rani Tiara Wandira Hariyanto Tita Damayanti Lestari Tyasningsih, Wiwiek Tyasningsih, Wiwiek Venri Novryantoro Vensko Zico Dandi Wahyu Pratama Wibawati, Prima Ayu Widjaja, Nanik Sianita Wijaya, Andi Yasmin Wijaya, Nanik Sianita Wiwiek Tyasningsih Wiwiek Tyasningsih Wiwik Misaco Yuniarti Woro Hastuti Setyantini Wudhu, R Zaksara Fero Ali Mutaqin Wulandari, Innah Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Yunus , Muchammad Yunus, Muchammad Zayyin Dinana Zulfanisa, Rizky Asrin Zulianti, Brillia