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All Journal Media Kedokteran Hewan Aceh Journal of Animal Science Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology LOSARI: Jurnal Pengabdian Kepada Masyarakat Bima Abdi: Jurnal Pengabdian Masyarakat Jurnal Kedokteran Hewan Journal of Fisheries & Marine Jurnal Agro Veteriner
Rahmahani, Jola
Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia 60115
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Social Sciences Transportation Veterinary Other

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Milk quality test using lactose, specific gravity and fat test approaches at the Kertajaya Village Cooperative Unit, Kediri Astuti, Elita Sriwidya; Effendi, Mustofa Helmi; Rahmahani, Jola; Suwarno, Suwarno; Sabdoningrum, Emy Koestanti; Tacharina, Martia Rani
Jurnal Agro Veteriner Vol. 8 No. 2 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i2.74045

Abstract

The quality of fresh milk based on SNI 2011 regarding fresh milk requirements includes physical, chemical and the number of microorganisms. One of the physical properties of milk can be seen from the density test. The chemical properties of milk can be seen from the lactose and fat rate. The lactose test is by means of titration of the filtrate resulting from filtering milk which is free from any content other than lactose. Density test using a lactodensimeter. Fat test using the Gerber method. From the results of research on the quality of cow's milk which was carried out using the lactose test approach, density and fat obtain average results of 3.98% of lactose rate, 1.0259 g/ml of density and 2.7% of the at rate in VUC Kertajaya is good and close to the criteria according to SNI 2011. This can affect the selling price of milk from farmers in VUC Kertajaya.
Impact of different antibiotic treatments on pregnancy maintenance in Holstein Friesian cows infected with foot-and-mouth disease Vensko Zico Dandi Wahyu Pratama; Jola Rahmahani; Herry Agoes Hermadi; Nusdianto Triakoso; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.34-38

Abstract

This study was conducted at Setia Kawan dairy cattle farming cooperative in Nongkojajar, Pasuruan, East Java to evaluate the effects of different antibiotic treatments on pregnancy sustainability in Holstein Friesian (HF) dairy cows infected with foot and mouth disease (FMD). The objective was to identify which antibiotics have minimal impact on pregnancy outcomes in FMD infected cows. A total of 106 pregnant HF cows infected with FMD were included in this study. Samples were selected using a purposive sampling method with inclusion criteria of pregnant HF dairy cows that tested positive for FMD. Four types of antibiotics were assessed, including enrofloxacin, oxytetracycline, amoxicillin, and penicillin-streptomycin. The results showed that among cows treated with enrofloxacin, 88.46% maintained pregnancy while 11.54% experienced abortion. For oxytetracycline, 73.08% of cows did not abort, whereas 26.92% did. In the amoxicillin group, 61.54% maintained pregnancy and 38.46% aborted. For penicillin-streptomycin, only limited data were available, with 23.07% maintaining pregnancy and no recorded cases of abortion. In conclusion, while variations were observed among different antibiotic treatments, statistical analysis indicated no significant association between antibiotic type and abortion rate in HF cows infected with FMD. Further studies with larger sample sizes are recommended to confirm these findings.
First case report of Salmonella spp. infection in Homalopsis buccata snake: Laporan Pertama Kasus Infeksi Salmonella spp. pada Ular Homalopsis buccata Tiara Wandira Hariyanto; Amelia, Ezanti Nur; Tyasningsih, Wiwiek; Rahmahani, Jola; Praja, Ratih Novita; Yudhana, Aditya; Nurwartanti, Maya; Fikri, Faisal
Media Kedokteran Hewan Vol. 34 No. 2 (2023): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v34i2.2023.128-134

Abstract

Reptiles are a source of transmission of pathogenic bacteria to humans. Salmonella spp. are Gram-negative bacteria that are commonly found in the digestive tract of snakes. This study was conducted on Homalopsis buccata snakes with samples obtained by oral swabs and cloacal swabs on snakes. Isolation of Salmonella spp. carried out on Tetrathionate Broth media enrichment and will be inoculated on specific media in the form of Salmonella Shigella Agar media. Identification of bacteria was carried out by Gam staining and Indole, Methyl Red, Voges Proskauer and Citrate test. This study revealed the presence of Salmonella spp. in the snake Homalopsis buccata.
The Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction Pinayungan, Probo Probo Warih Tatag; Aprinda Ratna Lovela; Eduardus Bimo Aksono; Ismudiono; Boedi Setiawan; Kadek Rachmawati; Jola Rahmahani
Media Kedokteran Hewan Vol. 35 No. 1 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i1.2024.21-27

Abstract

Sex identification of Sun Conures (Aratinga solstitialis) is crucial for breeding and preservation, as well as increasing sun conure populations. These birds are sexually monomorphic. Therefore, Determination between male and female carried out by their morphology examination. The Polymerase Chain Reaction (PCR) method, utilizing molecular-based technology, was employed to determine the sex of Aratinga solstitialis in this study. The P2 and P8 primers were utilized in this method, which has been deemed suitable and accurate for sex identification through calamus samples. The research focused on two 28-month-old Aratinga solstitialis birds. Calamus samples were collected and subjected to PCR amplification using the extracted calamus. The resulting PCR products were then visualized using electrophoresis with a 1% agarose gel. In the electrophoresis photo, the presence of two bands indicated a female specimen, whereas a single band indicated a male specimen. The result of the gel electrophoresis research showed that both of the Aratinga solstitialis were male with one band of each bird on ranged from 300-400 base pairs. The result show that the Polymerase Chain Reaction method in terms for sex identification on monomorphic birds, especially Aratinga solstitialis birds is very effective to differentiate the sex of young birds and the adults.
Designing of a Novel Aerolysin-based Multiepitope Vaccine against Aeromonas hydrophila Isolated from Osphronemus goramy Using Reverse Vaccinology: an in Silico Approaches Rozi; Tyasningsih, Wiwiek; Rahmahani, Jola; Aksono, Eduardus Bimo; Yunus , Muchammad; Al-Arif, Mohammad Anam; Kuncorojati, Suryo; Kusdarwati, Rahayu; Sari, Putri Desi Wulan; Amal, Mohammad Noor Azmai; Salleh, Annas; Khanand, Nadeem; Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.62035

Abstract

Graphical Abstract Highlight Research The study aims to develop a multi-epitope vaccine (MEV) against A. hydrophila by targeting the aerolysin toxin, a key virulence factor responsible for infections in fish and humans. Computational methods identified and optimized B-cell and T-cell epitopes, focusing on their ability to trigger immune responses without causing toxicity or allergenicity. In silico simulations demonstrated that the MEV has a strong binding affinity to immune receptors like TLR-4, MHC-I, and MHC-II, indicating its potential to induce robust cellular and humoral immunity. Structural analysis of the MEV showed a stable 3D conformation, with most residues in favorable regions, ensuring stability during immune activation. The MEV could enhance disease control in aquaculture and reduce human infection risks, offering a promising solution to address antibiotic resistance and the absence of effective vaccines. Abstract Aeromonas hydrophila, gram-negative, is a major pathogen responsible for various diseases in mammals, reptiles, amphibia, and vertebrates, including fish and humans. Targeting the specific toxin aerolysin in A. hydrophila is crucial to address antibiotic resistance and the lack of adequate and protective vaccines against this intracellular pathogen. This study aimed to identify a multi-epitope vaccination (MEV) candidate targeting A. hydrophila aerolysin toxin to combat the disease effectively. Standard biochemical characterization methods and sequencing of the 16S rRNA, rpoB, and aerA genes identified the isolate AHSA1 as A. hydrophila. Subsequently, we identified B and T cell epitopes on the aerolysin protein and separately predicted MHC-I and MHC-II epitopes. The epitopes are then evaluated for toxicity, antigenicity, allergenicity, and solubility. The vaccine design integrated multi-epitope-based constructs, utilizing specialized linkers (GPGPG) and EAAAK linkers to connect epitope peptides with adjuvants in the cholera toxin B component, thereby enhancing immunogenicity. Ramachandran plots showed that 85.25% of the residues were located in the most favorable regions, which was followed by the generously allowed zone (1.30%), the additional allowed regions (10.80%), and the forbidden regions (2.65%), thus confirming the feasibility of the modeled vaccine design. Based on docking simulations, MEV had the highest binding and interaction energies with TLR-4, TLR-9, MHC-I, and MHC-II (-1081.4, -723.2, 866.2, -9043.3 kcal/mol). Based on computational modelling, we expect the Aerolysin MEV candidate design to activate diverse immune mechanisms, stimulate robust responses against A. hydrophila, and maintain safety. The significant solubility, absence of toxicity or allergic response, and minimal side effects in animal testing all contribute to the potential clinical utility of this vaccine candidate.  
CALIFORNIA MASTITIS TEST AND IDENTIFICATION OF ANTIBIOTIC-RESISTANT BACTERIA ON DAIRY CATTLE IN "KUD” SEMEN GANDUSARI SUBDISTRICT, BLITAR RAHMAHANI, JOLA; Puspitasari, Yulianna; Suwarno
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 8 No. 1 (2024): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v8i1.2024.096-105

Abstract

Mastitis is an inflammation of the udder that often affects dairy cows in the production phase. The impact of mastitis losses is quite large, including a drastic decrease in milk quantity and quality. Mastitis can be caused by antibiotic-resistant bacteria in dairy cows, including Escherichia coli, Staphylococcus spp or Streptococcus spp. The results of a recent study stated that the prevalence of mastitis in Indonesia reached 83%. The Coliform Mastitis Test (CMT), used worldwide for early detection of mastitis, has a specificity of 80.6% and a sensitivity of 82.4%. Semen Village Unit Cooperative (KUD) is a cooperative that is the center of milk production in Blitar District, which accommodates the milk of dairy cows from smallholder farms in Blitar District. The high incidence of mastitis caused by the presence of bacteria resistant to antibiotics encourages counseling on mastitis and identifying bacteria that cause antibiotic resistance. Extension activities are carried out by presenting material about mastitis caused by the presence of bacteria resistant to antibiotics with lecture techniques (PowerPoint slides), videos, questions, and answers, and demonstrating the California Mastitis Test on milk samples as an early detection test, as well as identifying bacteria that cause antibiotic resistance. This series of counseling activities is an effort to reduce the incidence of mastitis through increased knowledge and understanding of the community.
Application of Spa-Gene as A Marker of Molecular Epidemiology in the Case of Methicillin-Resistant Staphylococcus Aureus (MRSA) Which its from Dairy Cows in Surabaya Region Rizky, Asri; Effendi, Mustofa Helmi; Rahmahani, Jola
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.4817

Abstract

The aim of this study was to isolate and identify the strain of methicillin-resistant Staphylococcus aureus (MRSA) from cows milk in Surabaya and to determine the fragment Spa-Gene in MRSA strains. There were 50 samples of cows milk obtained from four dairy farms. From 50 tested samples, 19 samples (38%) were positive for Staphylococcus aureus. Antibiotic sensitivity test using oxacillin and erythromycin showed that 6 samples were resistant to the antibiotic oxacillin and 6 samples were resistant to erythromycin. MRSA confirmation tests that were conducted on 6 samples revealed 5 positive results for the MRSA strain. Electrophoresis of Polymerase Chain Reaction (PCR) product showed that 4 out of 5 samples were positive for the presence of the Spa-Gene fragment. The research results showed that there were 3 models of Spa-Gene fragments; the first had a length of 90 bp and 140 bp, the second had a length of 140 bp, and third had a length of 90 bp. This study revealed the nature of Spa-Gene polymorphism of MRSA strains isolated from milk samples. It was concluded that the Spa-Gene can be used as a molecular epidemiological marker of the MRSA strain.
Evaluasi Penyuluhan Penyakit Infeksius dan Reproduksi Ternak di Blitar Raya Suwarno, Suwarno; Safitri, Erma; Rahmahani, Jola; Rahmawati, Kadek; Supriyadi, Supriyadi; Pasang, Gabriel Sampe; Irfani, Dyah; Kurniasari, Pitaloka
Bima Abdi: Jurnal Pengabdian Masyarakat Vol. 5 No. 2 (2025): Bima Abdi: Jurnal Pengabdian Masyarakat
Publisher : Yayasan Pendidikan Bima Berilmu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53299/bajpm.v5i2.1578

Abstract

Blitar Raya (Kota dan Kabupaten) merupakan salah satu kantong ternak di Jawa Timur. Merebaknya kasus PMK (Penyakit Mulut dan Kuku) dan LSD (Lumpy Skin Disease) menyebabkan turunnya populasi ternak. Kasus yang dihadapi pemerintah setempat masih adanya kasus PMK dan LSD meskipun vaksinasi dan biosekuriti sudah dilakukan. Dampak lain akibat kedua jenis penyakit tersebut adalah menurunnya produksi susu dan gangguan reproduksi. Pengabdian kepada Masyarakat ini dilakukan untuk memberikan penyuluhan terkait materi tentang Virologi (program vaksinasi dan biosekuriti) dan Reproduksi ternak (deteksi birahi dan repeat breeding). Peserta kegiatan ini adalah para Dokter Hewan dan Paramedis yang ada di wilayah Blitar Raya. Ceramah dan diskusi dilakukan dengan mengundang para peserta untuk mengikuti kegiatan penyuluhan di ruang pertemuan RM Bu Mamik Kota Blitar. Materi disajikan dalam bentuk power point menggunakan LCD. Di akhir diskusi peserta diberikaan soal dalam bentuk kuesioner, dengan topik materi tentang Virologi dan Reproduksi. Topik materi Virologi dibagi lagi menjadi subtopik vaksinasi dan biosekuriti, sedangkan topik materi Reproduksi dibagi menjadi subtopik deteksi birahi dan repeat breeding. Penilaian kuesiononer didasarkan atas jawaban benar-salah dan kemampuan peserta di dalam menganalisis topik dan subtopik yang diberikan. Penilaian didasarkan atas pengetahuan peserta beda strata pendidikan terhadap materi yang diujikan. Kesimpulan dari hasil penilaian adalah strata pendidikan dan pengalaman lapangan memiliki peran penting di dalam menguasai topik materi penyuluhan.
Oil-Adjuvanted Polyvalent Formalin-killed Aeromonas hydrophila Vaccine Enhances Agglutinating Antibodies, Respiratory Burst, and Survival in Giant Gourami Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 18 No. 1 (2026): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i1.82866

Abstract

Graphical Abstract  Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture.   Abstract Motile Aeromonas septicaemia (MAS), predominantly associated with Aeromonas hydrophila, remains a major constraint in giant gourami (Osphronemus goramy) aquaculture. This study evaluated formalin-inactivated A. hydrophila vaccines prepared from MAS-associated field isolates, comparing a monovalent formulation (P2), a non-adjuvanted polyvalent formulation (P3), and an oil-adjuvanted polyvalent formulation (P4) against PBS controls (P1). A total of 240 fish were used (60 per treatment) and assigned to two parallel cohorts (immunology and survival/challenge). Immune endpoints (agglutinating titres, NBT activity, and splenic il-1β and ifn-γ transcription) were assessed on days 7, 14, 21, 35, and 42 post-vaccination. The survival cohort was challenged intraperitoneally at day 21 with a homologous A. hydrophila strain and monitored for 14 days post-challenge. Vaccination was clinically well tolerated and improved survival relative to controls, with P4 showing the highest protection (RPS 81.8%). Agglutinating titres differed by treatment and time; at the peak sampling point (day 35), mean titres in P4 were ~200-fold higher than in P1, and model contrasts indicated significant differences versus controls (p<0.001). Splenic il-1β and ifn-γ transcript levels were higher in vaccinated groups than in controls at later time points. These findings support further evaluation of an oil-adjuvanted polyvalent inactivated A. hydrophila vaccine for gourami, including dose optimisation, extended safety assessment, heterologous challenge, and field validation.
Co-Authors . Suwarno A'la, Rofiqul A. T. Soelih Estoepangestie Adikara, Tatang Santanu Agustono, Bodhi Akbar, Daniswara Irwanza Akhmad Taufiq Mukti Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Ali Agus Amal, Mohammad Noor Azmai Amelia, Ezanti Nur Annas Salleh Aprinda Ratna Lovela Ardhanella, Sellianova Arif Nur Muhammad Ansori Asri Rizky Asri Rizky Astria Novitasari Nidom Astuti, Elita Sriwidya Aswin, Ahmad Azahro, Aisyah Aziz, Githa Nurma Balqis Afifah Bambang Sektiari Lukiswanto Baroroh, Diana Nova Boedi Setiawan BUDI UTOMO Christian Marco Hadi Nugroho Chusniati, Sri Daruti Dinda Nindarwi Dhani Prakoso Didik Handijatno Dita Wisudyawati Diyantoro Diyantoro, Diyantoro Elisa Herina Dimariwu Emy Koestanti Sabdoningrum Erma Safitri Ernawati, Rahayu Fachriza Tri Aprilia Fachrur Rozi Fadlilah, Shinta Levea Ni'matul Faisal Fikri Fedik Abdul Rantam Fidyah Fitrawati, Fidyah Gazali Salim Haeriah Hani Plumeriastuti Helen Susilowati, Helen Herry Agoes Hermadi Hutahaean, Ryan David Pandapotan Imam Mustofa IMAM MUSTOFA Indah Rahmawati Ine Karni Ira Sari Yudaniayanti Irfani, Dyah Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Karo-karo, Desniwaty Khairullah, Aswin Rafif Khanand, Nadeem Kirana, Maha Kuncoro Puguh Santoso Kuncorojakti, Suryo Kuncorojati, Suryo Kurniasari, Pitaloka Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lucia Tri Suwanti, Lucia Tri Meles, Dewa Ketut Mohammad Noor Amal Azmai Mohammad Sofi’ul Anam Muchammad Yunus Muhammad Arief Muhammad Khaliim Jati Kusala Muhammad, Abdul Latif Mustofa Helmi Effendi Nenny Harijani Nidom, Chairul Anwar Nina Nurmalia Dewi Nor Laela Ramadhaniyah Nurwartanti, Maya Nusdianto Triakoso Pasang, Gabriel Sampe Pinayungan, Probo Probo Warih Tatag Pradhita, Dewanggi Kristi Sasmi Praja, Ratih Novita Prestalia Dwi Rachmawati Putih, Nurvita Putri Desi Wulan Sari Putri, Diana Novita Rahaju Ernawati Rahmawati, Anlisia Rahmawati, Kadek Rahmi Sugihartuti Ramandinianto, Sancaka Chasyer Ratna Damayanti Reviany Vibrianita Nidom Rimayanti Rimayanti Rosyta, Pegy Rozi Salleh, Annas Santanumurti, Muhammad Browijoyo Sari, Putri Desi Wulan Setyantini, Woro Hastuti Setyarina Indrasari Siti Komariyah Soeharsono Soeharsono Soeharsono Supriyadi Supriyadi Suwarno Suwarno Suwarno Suwarno Suwarno Tacharina, Martia Rani Tiara Wandira Hariyanto Tita Damayanti Lestari Tyasningsih, Wiwiek Tyasningsih, Wiwiek Venri Novryantoro Vensko Zico Dandi Wahyu Pratama Wibawati, Prima Ayu Widjaja, Nanik Sianita Wijaya, Andi Yasmin Wijaya, Nanik Sianita Wiwiek Tyasningsih Wiwiek Tyasningsih Wiwik Misaco Yuniarti Wudhu, R Zaksara Fero Ali Mutaqin Wulandari, Innah Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Yunus , Muchammad Yunus, Muchammad Zayyin Dinana Zulfanisa, Rizky Asrin Zulianti, Brillia