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Study of NTF-3 (Neurotrophic Factor 3) Gene Information in Columbidae by In Silico Suprianto, S.; Budiarsa, I Made; Dhafir, Fatmah; Permatasari, Ni Kadek Dewi; Safitri, Gusti Ayu Kadek Yunita
BERKALA SAINSTEK Vol 9 No 3 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i3.24163

Abstract

Neurotrophin Factor 3 (NTF3) is one of the genes that plays an important role in the regulation of the neural systems of vertebrate animals, this gene has a special function in explaining the survival factors of some vertebrate animals. Based on the information obtained from GenBank, the nucleotide sequence of the NTF-3 gene in several vertebrate animals has been known and some of the data obtained have not been studied further for research purposes in adding information related to the molecular character of the NTF-3 gene, such as the NTF-3 gene in Columbidae. Columbidae is a group of birds that have quite diverse species variations, the number of species in columbidae will be very helpful in obtaining data on comparisons of the genetic character of the NTF3 gene. The purpose of this study was to analyze and describe the information on the NTF-3 gene (Neurotrophic Factor 3) in Columbidae through the in silico approach with computational methods. The NTF3 gene nucleotide sequences in Columbidae showed a fairly high level of similarity to the base sequences. This illustrates the fairly close proximity between each species. Geotrygon Montana is a species of Columbidae which has variations of the Base sequence which is quite different from other species. Evaluation of the model structure shows good stability of each target protein, all evaluation results describe a good structure, meaning that the conformation of each target sequence is in accordance with the sequence, so that the structure that is built has high accuracy with the actual model. The results of this research study can be a special description in explaining the genetic characteristics of several Columbidae species for the purposes of conservation measures or efforts to preserve Columbidae species at the molecular and population genetic levels.
Effectiveness of Aloe Vera Extract (Aloe vera L.) Against Colletotrichum capsici Fungi and Its Use as a Learning Media Elza, Tiara; I Nengah Kundera; I Made Budiarsa
Equator Science Journal Vol. 2 No. 3 (2024): Equator Science Journal (ESJ)
Publisher : CV. Dharma Samakta Edukhatulistiwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61142/esj.v2i3.148

Abstract

This study aims to determine the effectiveness of aloe vera extract (Aloe vera L.) against Colletotrichum capsici fungus and determine the optimal concentration of aloe vera extract that effectively inhibits the growth  of Colletotrichum capsici fungus and is used as a learning medium. This study used a laboratory experiment method using a Complete Random Design (RAL) with 5 treatments and 4 replicates. The treatment consisted of a concentration of 0% as a negative control, a concentration of aloe vera extract of 25%, 50%, 75% and 100%. The observed parameter is the diameter of the formed inhibition zone. The results of the study used Variant Analysis (ANOVA) and continued with the smallest real difference (BNT) test. The results showed that the administration of aloe vera extract (Aloe vera L.) could inhibit the growth of Colletotrichum capsici fungus at a maximum concentration of 100%. The higher the concentration of aloe vera extract, the higher its ability to inhibit the growth of Colletotrichum capsici fungus. The results of this study are feasible to be implemented in the form of  a digital flipbook as a learning medium with a feasibility of 83.2%.
Penguatan literasi kesehatan lingkungan melalui sosialisasi resistensi Bakteri Endogen pada kaki Kecoak di Kelurahan Tondo Kundera, I Nengah; Budiarsa, I Made; Mawaddah, Hayyatun; Rauf, Abd
ABSYARA: Jurnal Pengabdian Pada Masayarakat Vol 5 No 2 (2024): ABSYARA: Jurnal Pengabdian Pada Masyarakat
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29408/ab.v5i2.28685

Abstract

The community in RT.05/RW.13, Tondo Village, Mantikulore District, Palu City, shows a low level of awareness in maintaining environmental cleanliness, particularly in managing household waste. This condition contributes to the spread of pathogenic bacteria through cockroach vectors (Periplaneta americana), which can cause various diseases. This community service program aimed to enhance public health literacy regarding environmental cleanliness, the resistance of endogenous bacteria carried by cockroaches, and to establish the sustainability of environmental health literacy. The program applied the Participatory Action Research (PAR) method, consisting of four stages: to know, to plan, to act, and to change. The program involved 24 residents of RT.05/RW.13 and was conducted on July 26-27 and August 3-5, 2024. Results indicated an improvement in public understanding, with questionnaire results showing an 84% score categorized as good. Additionally, an educational poster about the dangers of cockroaches as disease vectors and their bacterial resistance was developed and installed in the community area. This increase in health literacy is expected to motivate behavioral changes among residents in maintaining environmental cleanliness, ultimately supporting the creation of a healthier environment
Problem Based Learning Assisted by the Padlet Application on Critical Thinking Abilities and Collaboration Skills Yuni Masyita Dewi; Astija; I Made Budiarsa
Journal of Education Action Reseach Vol 8 No 1 (2024): February 2024
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jear.v8i1.77566

Abstract

Critical thinking and collaboration skills are essential for students to face the challenges of the 21st century. However, currently, there are still many students who need more critical thinking abilities and collaboration skills, resulting in learning objectives not being achieved optimally. Based on this, this research aims to analyze problem-based learning assisted by the Padlet application on Critical Thinking Abilities and Collaboration Skills. This research is quantitative with a quasi-experimental type of research. This research design uses a non-equivalent control group consisting of experimental and control groups. The sample for this research is the experimental class of 35 students and the control class of 35 students. This research uses data collection techniques, including tests and observations. The data analysis techniques used are the normality test, homogeneity test, and ANOVA test. The results of this research show problem-based learning, assisted by the Padlet application, has a significant influence on critical thinking abilities and collaboration skills. It was concluded that problem-based learning assisted by the Padlet application can improve students' critical thinking abilities and collaboration skills.
Efectiveness of Shallot Root Extract Local Palu Against Candida albicans Fungus and its Utiliation as Learning Media Maharani, Mirfadz; Kundera, I Nengah; Budiarsa, I Made
Equator Science Journal Vol. 3 No. 1 (2025): Equator Science Journal (ESJ)
Publisher : CV. Dharma Samakta Edukhatulistiwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61142/esj.v3i1.149

Abstract

The potential of Palu local shallot roots as an antifungal agent has not been optimally utilized. The aim of this research is to determine the effectiveness of Palu local shallot root extract against the fungus Candida albicans, identify the optimal concentration for inhibiting the growth of Candida albicans, and to use the findings as an educational medium. This research employed a laboratory experimental method with a completely randomized design (CRD) consisting of 5 treatments and 4 replications at concentrations of 0% as a negative control, 25%, 50%, 75%, and 100%. The Candida albicans fungus was obtained from Paramartha Lab Mojokerto with strain code ATCC-14053. The parameter observed was the diameter of the inhibition zone formed. The data were analyzed using Analysis of Variance (ANOVA), followed by a Least Significant Difference (LSD) test. The results of this study showed that the extract of Palu local shallot roots effectively inhibited the growth of Candida albicans* at a concentration of 100%. This research is feasible to be used as an educational medium in the form of a digital flipbook with an eligibility percentage of 87.08%.
THE WORD “SHOOT” ANALYZED BY NATURAL SEMANTIC METALANGUAGE Prabhaswari, Putu Indry; Budiarsa, Made; Puspani, Ida Ayu Made
International Journal of Language and Literature Vol. 5 No. 2 (2021)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijll.v5i2.23979

Abstract

This study is focusing on the mapping of word shoot meanings. The collected data was qualitatively analyzed, it was starting by reading some sources, finding a word to be analyzed, reading carefully the source text and the target text, taking note, analyzed the text by NSM approach by Wierzbicka (1999). The data in this writing was taken from Colour Magazine Garuda Indonesia and used one of the prevalent comic series of Tintin entitled The Adventures of Tintin: Tintin in America and Tintin the shooting star. The technique of this study were three steps, they are finding the word which want to analyzed, read the text carefully, finding the word with its translation which want to discuss, directly noted the word which was according to the topic. The result of this study showed that the findings of three meanings of one word shoot. Some of the translation could convey the meaning therefore the reader in target language is easily read and understood.
MODEL PENGEMBANGAN DESTINASI WISATA NIRWANA GARDEN BALI DI KABUPATEN JEMBRANA BERBASIS GREEN TOURISM DALAM MEWUJUDKAN PARIWISATA BERKELANJUTAN Arya Wahyu Widiantara, I Putu; Budiarsa, Made; Darsana, I Made
Berajah Journal Vol. 4 No. 11 (2025): Berajah Journal
Publisher : CV. Lafadz Jaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47353/bj.v4i11.523

Abstract

The decline in tourist visits and environmental management challenges at Nirwana Garden Bali highlight the need for a more sustainable development strategy. This study aims to analyze tourism development, identify the implementation of Green Tourism, and design a sustainable destination development model to enhance competitiveness and local community involvement. A qualitative approach was used to analyze the green tourism-based development model at Nirwana Garden Bali. The research was conducted in Jembrana Regency, Bali, with primary data collected through in-depth interviews, FGDs, observations, and open-ended questionnaires with managers, tourists, and local communities, while secondary data was obtained from official documents and scientific literature. The research instruments included interviews to explore management strategies, FGDs to assess social, economic, and environmental impacts, as well as observations and documentation analysis related to regulations. The findings indicate that Nirwana Garden Bali has the potential to become a sustainable tourism destination through the development of eco-friendly infrastructure and partnerships with local communities. The implementation of green tourism principles, such as energy efficiency, water management, and other environmentally friendly practices, supports sustainability. This development model adopts the Green Tourism Marketing (GTM) and Penthahelix concepts, which promote collaboration among various stakeholders to enhance destination competitiveness while preserving the natural and cultural heritage of Bali.
Analisis Karakter Morfologi sebagai Penentuan Hubungan Kekerabatan Jenis Tumbuhan Paku di Daerah Perkebunan Kelapa Sawit Mamuang Desa Lalundu Patigu, Rosi Feraningsih; Suleman, Samsurizal M.; Budiarsa, I Made
Journal of Biology Science and Education Vol. 7 No. 2 (2019): Desember
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/jbse.v7i2.1138

Abstract

Penelitian ini bertujuan untuk mendeskripsikan hubungan kekerabatan jenis tumbuhan paku (Pteridophyta) di daerah perkebunan kelapa sawit Mamuang desa Lalundu. Metode yang digunakan adalah metode survei dengan teknik pengambilan sampel yaitu koleksi bebas. Data diolah dengan menggunakan Program File Editor (PFE) kemudian dianalisis dengan program Multivariat Statistical Package (MVSP). Indeks similaritas dihitung menggunakan metode Simple Matching Coefficient dan penentuan hubungan kekerabatan menggunakan UPGMA algorithm. Hasil penelitian menunjukkan bahwa tumbuhan paku yang didapatkan ada 10 jenis di Perkebunan kelapa sawit Mamung desa Lalundu, yaitu Asplenium macrophyllum, Stenochlaena palustris, Pityrogramma calomelanos, Asplenium nidus, Vittaria elongat, Selaginella plana, Asplenium confusum, Drymoglossum piloselloides, Nephrolepis bisserata, dan Displazium esculentum. Kesepuluh spesies tumbuhan paku membentuk 9 klaster. Klaster pertama ditempati oleh Asplenium macrophyllum dan Asplenium confusum sedangkan 8 jenis lainnya masing-masing menempati klaster kedua sampai dengan klaster kesembilan. Hubungan kekerabatan terdekat terjadi antara spesies Asplenium macrophyllum dan Asplenium confusum dengan indeks similaritas 0,725 atau 72,50%, sedangkan hubungan kekerabatan terjauh antara individu dari spesies tumbuhan paku pada node tujuh (Asplenium confusum, Asplenium macrophyllum, Asplenium nidus, Nephrolepis bisserata dan Displazium esculentum) dengan spesies pada node 8 (Stenochlaena palustris, Pityrogramma calomelanos, Selaginella plana, Vittaria elongata dan Drymoglossum piloselloides) dengan indeks similaritas 0,446 atau 44,60%.
Kadar Protein Berbagai Jenis Kacang (Leguminoceae) dan Pemanfaatannya sebagai Media Pembelajaran Trianto, Manap; Budiarsa, I Made; Kundera, I Nengah
Journal of Biology Science and Education Vol. 7 No. 2 (2019): Desember
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/jbse.v7i2.1140

Abstract

Kacang-kacangan (Leguminoceae) merupakan salah satu bahan pangan nabati yang memiliki persentase ekonomis yang tinggi bahkan merupakan sumber protein. Penelitian ini bertujuan untuk mengetahui kadar protein aneka kacang-kacangan (Leguminoceae) dari Pasar Malam di Desa Sumbersari, Kecamatan Parigi Selatan, Kabupaten Parigi Moutong dan penerapannya sebagai media pembelajaran. Jenis penelitian ini adalah penelitian laboratorium eksploratif. Penelitian ini menggunakan analisis Kjedhal Makro dengan tiga bagian yaitu destruksi, destilasi dan titrasi pada empat macam sampel. Hasil penelitian menunjukkan bahwa kadar protein dari seperempat sampel sebesar 24,41%/gram sampel protein yang terdapat pada kacang tanah (Arachis hypogaea L.), kacang hijau (Phaseolus radiatus L.) mengandung 20,31%/gram protein sampel. , kacang merah (Phaseolus vulgaris L.) 16,42%/gram sampel protein dan kacang tunggak (Vigna unguiculata L.) mengandung 21,71%/gram sampel protein. Berdasarkan setelah melalui tahap validasi dari ahli isi, desain dan media dengan diperoleh 30 siswa, penelitian ini dapat dikategorikan sebagai media pembelajaran berupa poster dengan persentase 86,69%.
Prediksi Epitop untuk Desain Vaksin Virus Dengue secara In Silico Marisa, Fajri; Budiarsa, I Made; Kundera, I Nengah
Journal of Biology Science and Education Vol. 8 No. 1 (2020): Juni
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/jbse.v8i1.1158

Abstract

Penyakit akibat infeksi virus dengue menjadi salah satu masalah kesehatan utama di dunia, sehingga diperlukan suatu upaya pencegahan dengan cara pengembangan vaksin. Tujuan penelitian ini adalah untuk mendeskripsikan kandidat epitop yang layak digunakan dalam mendesain vaksin virus dengue secara in silico dan pemanfaatannya sebagai media pembelajaran bioinformatika berupa poster. Data dalam penelitian diperoleh melalui pencarian sekuen protein envelope virus dalam situs NCBI dan RCSB. Prediksi epitop sel T dan sel B dilakukan melalui server MULTIPRED2 dan ElliPro. Pembuktian epitop dilakukan dengan menyusun sekuen vaksin dan homology modeling struktur tiga dimensi vaksin. Evaluasi struktur tiga dimensi vaksin dilakukan dengan mengurangi residu asam amino yang tumpang tindih, menentukan skor QMEAN, nilai Root Mean Square (RMSD), skor Template Modeling (TM) dan menganalisis plot Ramachandran. Validasi vaksin dilakukan melalui server Verify3D. Hasil penelitian menunjukan kandidat epitop yang layak digunakan untuk mendesain vaksin virus dengue terdapat pada urutan asam amino LVTFKTAHA, IQMSSGNLL dan MILMKMKKK. Evaluasi vaksin menunjukkan tidak terdapat residu asam amino tumpang tindih dengan nilai RMSD dibawah 0,1, skor TM 0,999 dan jumlah residu non glisin yang kurang dari 15% total residu sekuen penyusun vaksin. Validasi sekuen dan struktur tiga dimensi vaksin mencapai 91.58% asam amino yang memiliki skor 3D-1D 0,2.
Co-Authors ., Reskiana Abd Hakim Laenggeng, Abd Hakim Abd Rauf Achmad Ramadhan Achmad, Mestawaty As. Ahmad Ramadhan Akram, Akram Amalia Buntu Amiruddin Kasim Anak Agung Putu Agung Suryawan Wiranatha Anak Agung Putu Putra Aro Meko Mbete Aron Meko Mbete Arya Wahyu Widiantara, I Putu Ashari, Abdul Astawa, I Gede Astija Avita, Cindy Dea Bahana Aditya Adnan Bialangi, Mursito S. Biung, Yelsan Kanan Burhanuddin, Alga Patricia Chatarina Umbul Wahyuni Denok Lestari Dewa Ayu Widiasri Dewi Tureni Dwi Lina Sari Tanjung Elza, Tiara Faradila, Ratna Fardini Sabilah Fatmah Dhafir Febri Aspitasari Gede Primahadi Wijaya Rajeg Hasan, Isharyadi Hasanudin, Rezki Hayyatun Mawaddah Herry Arianto Hidyanisa, Dhea Hj. Musdalifah Nurdin I Gde Pitana, I Gde I Gede Putra Nugraha I Gusti Ayu Gede Sosiowati I Gusti Ayu Krisnawati I Ketut Artawa I Ketut Murdana I Ketut Purwata I Ketut Riana I Ketut Wardana I Made Antara I Made Darsana I Made Netra I Made Rai Jaya Widanta I MADE RAJEG I Made Suastra I Nengah Kundera I Nengah Laba I Nengah Sudipa I Nyoman Sudiarta I Nyoman Sunarta I NYOMAN SUPARWA I Nyoman Udayana I WAYAN PASTIKA I Wayan Simpen I Wayan Sugita Ida Ayu Made Puspani Ida Bagus Putra Yadnya Ilahi, Wahyu Abdul Isninar, Isnainar Jesy Valenia Panggabean Kadek Feni Aryati Kadek Purnata Kusumaningati, Lilis Tri Latompai, Nismawati I. Lestari M.P Alibasyah Lilies Lilies Made Antara Made Antara Made Sri Satyawati Maharani, Mirfadz Manap Trianto Manap Trianto MARIA BINTANG Marisa, Fajri Masriani . Messe, Yunita Mohammad Jamhari Muchlis L. Djirimu Muh Annas Abdillah Muhammad F. Rahman Mursito S. Bialangi Nabila, Putri Nasution, Annio Indah Lestari Nasution, Rahmad Kurnia Abdik Ni Ketut Sri Rahayuni Ni Luh Nyoman Seri Malini Ni Luh Sutjiati Beratha Ni Luh Yuniarti Ni Made Dhanawaty Ni Made Suryati Ni Putu Herni Susanti Ni Wayan Sartini Novita Mulyana NOVITASARI Nur Alam Nurwahda, Nurwahda Nyoman Ariana Nyoman Surya Wijaya Patigu, Rosi Feraningsih Permatasari, Ni Kadek Dewi Prabhaswari, Putu Indry Puspitasari, Amira Putu Dewi Merlyna Yuda Pramesti Putu Prasanthi Vahini Putu Sandra Putri Astariani Rachmad Suwardi Rafiqa RAFIQA RAFIQA, RAFIQA Rahayu, Irma Septi rofiqoh rofiqoh Safitri, Gusti Ayu Kadek Yunita Salsabila Husna Ramadhani Amara Samsurizal, M. Sulaeman Saridewi, Uut Shamdas, Hj. Gamar B.N SULFIANTI, SULFIANTI Suprianto Suprianto Suprianto Susanti, Putu Herny Sutrisnawati Mardin Syamsul Alam Paturusi Syech Idrus Syech Zainal Tandi, Jenifa Arisa Thelisa . Tulak, David Pebriyanto Tuna, Anjeli Wayan Darma Sedana Widiastuti . Windarsih, Yulia Yafed Syufi Yafet Syufi Yuni Masyita Dewi Zulaikhah Dwi Jayanti