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All Journal VALENSI Teknologi dan Kejuruan: Jurnal teknologi, Kejuruan dan Pengajarannya JURNAL KIMIA SAINS DAN APLIKASI Jurnal Pendidikan Teknologi dan Kejuruan Al-Manahij: Jurnal Kajian Hukum Islam Media Penelitian dan Pengembangan Kesehatan agriTECH Jurnal Pendidikan Ilmu Sosial R.E.M (Rekyasa Energi Manufaktur) Jurnal Manajemen Pendidikan BERITA BIOLOGI E-Dimas: Jurnal Pengabdian kepada Masyarakat Edukasi: Jurnal Pendidikan Jurnal Pengembangan Teknologi Informasi dan Ilmu Komputer Jurnal Biodjati AL-IDARAH: JURNAL KEPENDIDIKAN ISLAM ANNALES BOGORIENSES Justek : Jurnal Sains Dan Teknologi Jurnal Agrotek UMMat SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Journal of Tropical Crop Science IZDIHAR Jurnal Pro Hukum : Jurnal Penelitian Bidang Hukum Universitas Gresik Al - Tadabbur: Jurnal Ilmu Al-Qur'an dan Tafsir GERVASI: Jurnal Pengabdian kepada Masyarakat Teknologi Indonesia JURNAL PANGAN Musamus Journal of Physical Education and Sport (MJPES) Jurnal Ekonomi Manajemen Sistem Informasi APTEK Rayah Al Islam : Jurnal Ilmu Islam Jurnal PORKES Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Teknik Industri Terintegrasi (JUTIN) SUHUF: Jurnal Pengkajian Al-Qur'an dan Budaya International Journal of Social Learning (IJSL) Journal La Lifesci International Journal of Progressive Mathematics Education JUSTIN (Jurnal Sistem dan Teknologi Informasi) Jurnal Agro Dedikasi Masyarakat (JADM) Journal of Office Administration : Education and Practice Jurnal Energi dan Inovasi Teknologi Indonesian Health Issue Tsaqofah: Jurnal Penelitian Guru Indonesia Jurnal Magister Akuntansi Trisakti Jurnal: International Journal of Engineering and Computer Science Applications (IJECSA) INJURITY: Journal of Interdisciplinary Studies Bulletin of Network Engineer and Informatics (BUFNETS) Journal of Educational Management Research PANDU: Jurnal Pendidikan Anak dan Pendidikan Umum Pengabdian Jurnal Abdimas JPMAT Prosiding Seminar Nasional Keperawatan Universitas Muhammadiyah Surakarta Al-Qudwah: Jurnal Studi Al-Qur'an dan Hadis El-Mujtama: Jurnal Pengabdian Masyarakat Cocos Bio Protech Biosystems Journal Journal of Multidisciplinary Inquiry in Science, Technology and Educational Research Jurnal Kefarmasian Indonesia Jurnal Pengabdian Dosen Republik Indonesia (JPDRI) Jurnal Ilmiah Cano Ekonomos Annales Bogorienses Berita Biologi
Ahmad Fathoni
Universitas Darussalam Gontor
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Journal : BERITA BIOLOGI

 1 
SKRINING DAN ISOLASI METABOLIT AKTIF ANTIBAKTERI KULTUR JAMUR ENDOFIT DARI TUMBUHAN Albertisia papuana Becc. [Screening and Isolation of Antibacterial Active Metabolite from the Culture of Endophytic Fungi from Albertisia papuana Becc.] Fathoni, Ahmad; Ilyas, Muhammad; Cahyana, Antonius Herry; Agusta, Andria
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8087.938 KB) | DOI: 10.14203/beritabiologi.v12i3.639

Abstract

Totally 15 isolates of endophytic fungi were obtained from leaves and young stems of a medicinal plant Albertisia papuana Becc.(Menispermaceae). The antibacterial screening of the ethyl acetate extract derived from the fungi cultures in potato dextrose broth (PDB)were performed on a non eluted Thin Layer Chromatography Bioautography assay (TLC bioautography assay). From the screening test, itwas found that the ethyl acetate extract of the fungus Xylaria sp. DAP-KRI-5 culture showed strong antibacterial activity against Staphylococcus aureus. The isolation of active metabolite based on the bioautography guided assay from ethyl acetate extract of the Xylaria sp. DAP-KRI-5, led us to identify phloroglucinol as a main antibacterial compound. The chemical structure of phloroglucinol was deduced from it spectral data, including UV-Vis, 1H and 13C-NMR, GC-MS and published data elsewhere.
SKRINING BEBERAPA JAMUR ENDOFIT TUMBUHAN DARI PULAU ENGGANO, BENGKULU SEBAGAI ANTIBAKTERI DAN ANTIOKSIDAN [Screening of Plant Endophytic Fungi from Enggano Island, Bengkulu for Antibacterial and Antioxidant Activites] wulansari, dewi; Putra, Aldho Pramana; Ilyas, Muhammad; Praptiwi, Praptiwi; Fathoni, Ahmad; Palupi, Kartika Dyah; Agusta, Andria
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3260.703 KB) | DOI: 10.14203/beritabiologi.v15i3.2259

Abstract

Fungal endophytes have been known as sources for bioactives with high chemical structure variability. This study aimed to screen extracts of some endophytic fungi associated with plants from Enggano Island as antioxidant and antibacterial using thin layer chromatography (TLC) bioautography method. Antibacterial ativity was performed against Escherichia coli and Staphylococcus aureus. Seventeen out of 22 extracts showed growth inhibition activity against both bacteria, while 16 extracts were active as DPPH free radical scavenging agents. Further determination on IC50 value of four prominent extracts revealed that fungal endophytes AK3018-1, FC-1, KC-4, and SO-3 have IC50 value of 85, 84, 704, and 347 ?g/ml, respectively in DPPH radical scavenging method. Extracts of fungal endophytes CR-3, CS-2, and SM-2 showed prominent antibacterial activity among other extracts, indicated by wide clear white zone around the spot. Further evaluation on minimum inhibitory concentration (MIC) of those three extracts by microdilution method showed that CR-3, CS-2, and SM-2 have MICs value of 512, >512, and 64µg/ml, respectively against S. aureus.Fungal endophytes AK3018-1, FC-1, KC-4, SO-3, CR-3, CS-2, and SM-2 were respectively isolated from Dioscorea bulbifera tuber, Fibraurea chloroleuca twig, Knema cinerea twig, Smilax odoratissima stem, Cryptocarya sp. twig, Calophyllum soulattri twig, and Smilaxmacrophylla stem.
EVALUASI ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SMILAX spp. DARI PULAU ENGGANO [Evaluation of Antibacterial and Antioxidant of Smilax spp. Extracts Collected from Enggano] Tiwi, Praptiwi; Palupi, Kartika Dyah; Fathoni, Ahmad; Keim, Ary P; Royani, M Fathi; Effendi, Oscar; Agusta, Andria
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3098.315 KB) | DOI: 10.14203/beritabiologi.v15i3.2213

Abstract

Three species of Smilax spp. (Smilax macrophylla, S. odoratissima and S. zeylanica) collected from Enggano island were evaluated for their potential as an antibacterial and antioxidant. Stems and leaves of three species of Smilax spp. were extracted successively with nhexane,chloroform, ethyl acetate and methanol. Antibacterial activity was evaluated by TLC-bioautography against Eschericha. coli InaCC B5 and Staphylococcus. aureus Ina-CC B4. The antioxidant activity was analyzed by DPPH free radical activity by bioautography method. The value of minimum inhibitory concentration (MIC) and IC50 of active extracts were done by serial dilution in 96- well microplate. The results showed that 20 extracts have antioxidant activity, 13 extracts inhibited the growth of S. aureus Ina-CC B4, and 14 extracts inhibited the growth of E. coli Ina-CC B5. MIC values of active extracts against S. aureus Ina-CC B4 were in the range of 128 - > 512 µg / ml, while the values of MIC against E. coli B5 Ina-CC were > 512 µg / ml. IC50 values of extracts that has antioxidant activity were in the range of 184.11-4549.34 mg/L.
EVALUASI AKTIVITAS ANTI-INFLAMASI DAN ANTIOKSIDAN SECARA IN-VITRO, KANDUNGAN FENOLAT DAN FLAVONOID TOTAL PADA TERMINALIA SPP. [Evaluation of In-vitro Anti-inflammatory and Antioxidant Activity, Total Phenolic and Flavonoic Contain on Terminalia spp.] Murningsih, Tri; Fathoni, Ahmad
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3007.604 KB) | DOI: 10.14203/beritabiologi.v15i2.2264

Abstract

Terminalia is the second largest genus of Combretaceae family, consists of 200 species distributed in the tropics and subtropics. Many species of Terminalia are well kown in traditional medicine  in several countries of Africa and Asia. In this study, bark extracts of four species of Terminalia (T. catappa, T. citrina, T. bellirica and T. macadamii) were evaluated for their in-vitro anti-inflammatory and antioxidant activity. Determination of the anti-inflammatory activity was carried out using red blood cell membrane stabilization assay and the antioxidant activity was carried out using DPPH free radical scavenging and ferric reducing power assay. Estimation of total phenolic   content was carried out using Folin-Ciocalteu reagent, while  total flavonoid content using alumminium trichloride reagent. The results showed that extract of T. catappa has the most potent anti-inflammatory activity (IC50= 97,83±0,0100 ?g/mL) and the most powerful  antioxidant activity of DPPH free radical scavenging (IC50= 21,8900 ± 0,0264 ?g/mL) and ferric reducing power (IC50= 87,1533 ± 0,04163 ?g/ mL). The weakest anti-inflammatory activity was T. citrina extract (IC50= 159,35±0,0200 ?g/mL), whereas T. bellirica extract demonstrated the weakest antioxidant activity of  DPPH free radical scavenging (IC50= 44,4867 ± 0,0153 ?g/mL) and ferric reducing  power (IC50= 140,89±0,04 ?g/mL). The extract of T. citrina has the highest content of total phenolic (84,8167 ± 0,53407 mg GAE/g) and flavonoid (23,1200 ± 1,7149 mg RE/g) while the T. macadamii extract has the lowest content of total phenolic (24,3700 ± 0,0173 mg GAE/g) and flavonoid (13,2667 ± 0,3386 mg RE/g).
SKRINING AWAL AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SEMUT (INSECTA: FORMICIDAE) DARI GARUT - JAWA BARAT Efendy, Oscar; Fathoni, Ahmad; Praptiwi, Praptiwi; Royyani, Mohammad Fathi; Wulansari, Dewi; Agusta, Andria
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3621

Abstract

Studies on the therapeutic use of insects and insect products have been neglected compared to the use of other animals or plants.This study aims to determine the antibacterial and antioxidant potential of ants extracts. Preliminary study related to antibacterial and antioxidant screeningof 17 extracts of ant colonies that belongs to 8 species were performed by Thin Layer Chromatography (TLC)-Bioautography. Antioxidant activity was measured by DPPH free radical scavenging method. The antibacterial activity was done against three pathogenic bacteria, i.e Bacillus subtilis InaCC B-1, Staphylococcus aureus InaCC B-4 and Escherichia coli InaCC B-5 were performed by non-eluted TLC-autobiography assay. The minimum inhibitory concentration (MIC) and IC50 values of DPPH radical scavenging activity of active extracts were determined by microdilution in 96-well microplate. The results showed 6 extracts active against B.subtilis, 5 extracts active against S.aureus. The lowest MIC value was 512 µg/ ml.  Ten extracts had the antioxidant activity with various IC50 values. The extracts of ants might be used as bioactive resources for antibacterial and antioxidant.  
Co-Authors -, Munoto Abdul Aziz Abdul Basid Abdul Hakim Abdul Malik Abdul Muhaimin, Abdul Abdul Wahab Rosyidi Adi Gunawan, Adi Adi Supratikto, Adi Ahmad Muhibbin Ahmad Zain Sarnoto Aini, Isnawatul Akbar, Muhammad Aminul Aldy, Aldy Alfan Habib, Muhammad Alfi Rahmi, Alfi Alfiah, Lufita Nur Alpiana, Alpiana Amilia, Nurul AMINUDIN ANDRIA AGUSTA Anjis Sapto Nugroho Anshori, Muhammad Helmi Anton Anton Antonius Herry Cahyana Aprilia, Aqsha Putri Aprizal Arginia, Della Arif Nurkanto Arif Rahman Saleh Arip, Moh Astuti, Tri Dwi Ayuniyyah, Q Azhar, Fachri Azis Hakim Azis, Sri Misykat Azmi Asra, Azmi Azmi, Aulul Baiq Desi Dwi Arianti Basirun, Basirun Dewi Sukma DEWI WULANSARI Dian Kristiawan Nugroho Dody Pratama Marumpe Durinda Puspasari Dwi Septyan , Ridta Efendi, Oscar Efendy, Oscar Effendi, Oscar Eko Supriyanto Ema Damayanti Emilyani, Desty Eri Zuliarso Erik Estrada Ernawati Sinaga Erni Romansyah Euis Ismayati Evana, Evana - Fahmi Sidik, Ali Faisal Mahmoud Adam Farooq, Omar Fatahillah, Ahmad Fathoni, Rois Fitriani Fitriani Fitriyanto, Arief Hafid, Lukman Haq, M. Ziyadul Hard, Rasyd Hariadi Hariadi Hariyanto Hengky Anra Heri Kuswanto Hidayatullah, Tobib Huda, Ade Naelul Huda, Ahmad Akromul Husni Tamim, Muhammad Ida Wahyuni Ikhsan Gunawan Indra Himayatul Asri Irdawati, I Irma Sari Ismatullah, Kholida Ismiyarto Ismitarto Iwan Kurniawan Subagja I’tishom Billah, Muhammad Qudwah Jamaludin . Jeong, Soo Kyoung Jeong, Soo Kyoung Jumesam, Jumesam Junaidin, Junaidin Kamal, Andi Saptaji Kamaludin, Basori Kartika Dyah Palupi Karyanik, Karyanik Keim, Ary P Khairunnisa, Imma Jannati Khoirul Muhtadin Kholisho, Yosi Nur Kim, Joon Kyun Kim, Joong Kyun Legisnal Hakim lina marlina, lina Maksum, M. Syukron Maliq, Nina Marianah, Marianah Markhamah Mawe, Hannatul Mayasti, Nur Kartika Indah Mohammad Fathi Royyani, Mohammad Fathi Mohammad Irfan Molo, Hasanuddin Muanah, Muanah Muhamad Arpan Muhammad Ilyas Muhammad Ramli Muliatiningsih Muliatiningsih Mustafidah, Mabrurotul Mu’iz Widayat, Walid N. Sri Hartati, N. Sri Naelul Huda, Ade Ngadiwiyana M.Si. S.Si. Nofriser, Nofriser Nunung Ariandani Nur Annisa Istiqamah Nur Halifa Nurhayati, Nurhayati Nurhikmah Sasna Junaidi Sasna Junaidi, Nurhikmah Sasna Junaidi Sasna Nurlaela, Lutfiyah Oktiva, Yayuk Dwi Pipit Irawati Prabowo, Ardian Adi Pramono Pramono Praptiwi Praptiwi Prastiwi, Erfa Kurnia Pratiwi Pratiwi Purwo Subekti Puspa, Rahma Putra, Aldho Pramana Putra, Reinaldo Dede Putri, Ade Lia Putu Sudira R. Haryo Bimo Setiarto, R. Haryo Bimo Raden Haryo Bimo Setiarto, Raden Haryo Bimo Rahmawati, Rika Sri Rina Nopiana Risna Rianti Sari Rois Fatoni Royani, M Fathi ryanjani lila anggraita SAKINAH, PUTRI Salma, Aninda Sandra Hermanto Saputra, Andra Sasna Junaidi, Nurhikmah Septiana, Nurwinda Sari Sintho Wahyuning Ardie Slamat, Slamat Sudarsono Sudiyanto, I wayan Suhairin, Suhairin Supari Muslim Suryansah Swahip, Swahip Syafri Edi Syamsiar, Syamsiar Syirril Ihromi, Syirril Tanjung, H Tarso Rudiana, Tarso Tiwi, Praptiwi Toman, Sarah Husain Tri Afirianto, Tri Tri Murningsih, Tri Tri Rijanto Tursina Tursina Uska, Muhammad Zamroni Visto, Yohanes Wahyudi Wahyudi Wariso Wariso, Wariso Wazni, M Khairul Wijoyo, Moh. Noer Tondo Winna Dharmayanti Yeza Febriani Yose Rizal Yuliasri Jamal Zaki Al Fatih, Moh Zakiy, Ahmad Zuliawan, Ikbal Zulkarnain, Lalu Iskandar