Claim Missing Document
Check
Articles

The Abnormality of Spermatozoa Goat after Freezing on -80°C Using Tris Diluent Added Combination Hatching Egg Yolk and Amnion Fluid Liza Choirun Nisa; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1051.185 KB) | DOI: 10.21776/ub.jels.2018.008.03.01

Abstract

This study aims to determine the combination of the hatching egg yolk (HEY) and amniotic fluid (AF) in Tris diluent to the type of goat spermatozoa abnormalities after frozen at temperature -80°C. This study used four treatments and 6 replications. The semen is diluted using tris extender and added treatment, there are control group (T0) (Tris egg yolk 20%), T1 (15% HEY + 5% AF), T2 (10% HEY + 10% AF), and T3 (5% HEY + 15% AF). After dilution, the semen was equilibrated for 2 hours, then stored using Mr. Frosty and frozen at temperature -80°C in the ultra-freezer for 24 hours. The type of spermatozoa abnormalities type was evaluated on fresh semen and after thawing. The data were analyzed using ANOVA (P<0.05). The results showed that there is no significant difference among treatments. The primary abnormalities in fresh semen, there were macro-cephalic, amorphous, and the secondary spermatozoa abnormalities such as detached head, mid-piece defect, coiled tail, loosehead, shoe-hook tail, and a broken tail. The secondary abnormalities in post-thawing were found both in the control group and treatment, which are detached head, loosehead, coiled tail, shoe-hook tail, and broken tail. The conclusion of this study was the percentage of secondary abnormalities after thawing was increasing both in the control and treatment groups. Keywords: Amniotic Fluid, Hatching Egg Yolk, Mr. Frosty, Type of Spermatozoa Abnormality.
The Effect of Morinda citrifolia L. Fruit Extract Supplemented in to Diluent Tris-Egg Yolk Toward the Abnormality of Goat's Spermatozoa after Freezing at -80°C Apriani Herni Rophi; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1116.681 KB) | DOI: 10.21776/ub.jels.2018.008.03.03

Abstract

The aim of this research is to know the effect of diluent Tris-egg yolk medium added with Morinda citrifolia L. extract towards the sperms abnormality after storage at -80°C for 24 hours. The extracts added in diluent Tris-egg yolk medium were 0% (P0), 10% (P1), 20% (P2), and 30% (P3). The result showed that the percentage of sperms abnormality in P0, P1, P2, and P3 were respectively 8.67%, 6.67%, 5.67%, and 10%. P2 was the lowest percentage, while P3 was the highest percentage of sperms abnormality. In addition, this research found twelve kinds of sperms abnormality divided into categories: major abnormality included the broke tail, absent head, detached head, dag defect, tapered head, proximal droplet, round head and abaxial; and minor abnormality included the coiled tail, shoe hook tail, bent tail, and distal droplet. It has been concluded that the allotment of 20% Morinda citrifolia L. extracts in Tris-egg yolk medium able to maintain the sperm morphology at -80°C storage for 24 hours. Keywords: goat, Morinda citrifolia L., post-thawing, spermatozoa.
Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study Siska Nanda Widhaningrum; Septiawan Putranto; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.06

Abstract

Water clover (Marsilea crenata) is one of the herbal plants that has been using in alternative medicine. It possesses pharmacologically active compounds like flavonoid, which has cellular activities such as antioxidant and estrogenic activity. This study aimed to evaluate the impact of water clover ethanol extract (WCE) at different concentrations on the growth of follicles and oocytes based on follicles and oocytes diameter, respectively, after six days of culture. This experimental study used 24 isolated antral follicles (2.5-3.2 mm), which were randomly divided into four groups including control (without supplemented WCE) and experimental groups that supplemented with different concentrations of WCE (21.6, 43.2, and 86.4 µg ml-1) in culture medium for six days culture. The diameter of follicles was measured on days 0, 3, and 6. Additionally, oocytes diameter was also measured on day 6. The results indicate that the mean diameter of antral follicles and oocyte diameter of WCE 43.2 µg ml-1 was significantly increase compared to the other groups (P≤0.05). According to our results, WCE exerts its effect on the growth of the antral follicle and oocyte based on follicles and oocytes diameter respectively in a dose-dependent manner after six days of the antral follicle cultured.  Keywords: antral follicle, flavonoid, in vitro, oocyte, water clover.
Study Motility of Ongole Crossbred Semen with Soybean-Based Diluents Stored at Ambient Temperature (24-27 0C) Dian Ratnawati; Gatot Ciptadi; sri rahayu; trinil susilawati
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 1 (2023): April 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.01.011

Abstract

Artificial insemination with fresh semen is an alternative that can be done if there is a limitation of liquid nitrogen, bull, and facilities for processing liquid and frozen semen (costly). This study aimed to determine the optimal diluent formula based on CEP-3 (modified/m) and aqueous soybean extract (ASE) to support spermatozoa motility at ambient temperature storage. The research design was completely randomized, with eight treatments and six replications. The diluent formula treatments were as follows: T1 (CEP-3(m)), T2 (CEP-3(m)+10% Egg Yolk/EY), T3 (CEP-3(m)+ASE 1%), T4 (CEP-3 (m)+ASE 2%), T5 (CEP-3(m)+ASE 3%), T6 (CEP-3(m)+ASE 4%), T7 (CEP-3(m)+ASE 5%), and T8 (CEP-3(m)+ASE 6%). The parameters were measured, i.e., the motility of spermatozoa and its kinetic parameters. Data were analyzed using ANOVA with SPSS 16 program. The result showed that the progressive motility of spermatozoa at day one was more than 50% in diluents CEP-3(m)+ASE 1-2%. Diluent CEP-3(m) and aqueous soybean extract 1-2% can support spermatozoa's motility and kinetic parameters during storage at ambient temperature, including progressive motility, motility, motility, velocity, and BCF.
Rancang Bangun Rumah Pengering Kopi Hybrid Guna Meningkatkan Efektivitas Pengeringan Kopi Di Kecamatan Pinogu, Provinsi Gorontalo Bambang Susilo; Gatot Ciptadi; Kiki Fibrianto; Hendrix Yulis Setyawan; Aris Subagyo; Rizki Putra Samudra; Roy Ardy Colas Napitupulu
Journal of Innovation and Applied Technology Vol 9, No 1 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2022.009.01.1

Abstract

Kopi telah menjadi produk unggulan Kecamatan Pinogu dengan cita rasa yang khas dengan pengolahannya yang alami membuat kopi ini dikategorikan sebagai kopi organik sehingga memiliki nilai jual yang tinggi. Permasalahannya, proses pengeringan kopi dengan sinar matahari sering terkendala cuaca sehingga produk yang dihasilkan kurang maksimal. Oleh karena itu, perlu dikembangkan teknologi tepat guna berupa rumah pengering hybrid. Rancang bangun rumah pengering berbahan baja dan polikarbonat diharapkan dapat mempercepat waktu pengeringan. Hasilnya didapatkan efisiensi waktu pengeringan menjadi 5,6 kali lebih cepat daripada metode konvensional, dan juga dapat menghemat ruang, serta mampu mengeringkan kopi sebanyak 240 kg sekali proses. Selain itu, pengeringan menggunakan metode ini juga dapat mengurangi kontaminasi pada kopi, sehingga dapat meningkatkan kualitas kopi.
ANALISIS KEMIRIPAN GENETIKA ANTARA KAMBING PERANAKAN ETTAWA HASIL KAWIN ALAM DENGAN INSEMINASI BUATAN BERDASARKAN RAPD Mudawamah M; I.D. Retnaningtyas; M.F. Wadjdi; Badriyah B; S. Susilowati; Aulanni’am A; Gatot Ciptadi
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.01 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2636

Abstract

Analisis genetika menggunakan random amplified polymorphic DNA (RAPD) untuk mengetahui kemiripan genetika antara kambing peranakan Ettawa (PE) hasil inseminasi buatan (IB) dan kawin alam (KA). Hasil penelitian menunjukkan bahwa dari 20 primer OPA hanya 10 primer yang bisa digunakan untuk mengamplifikasi fragmen deoxyribonucleic acid (DNA) dalam kelompok kambing PE, yang terdiri atas 21 kambing hasil KA dan 19 kambing hasil IB. Berdasarkan profil DNA yang diperoleh dan hasil analisis dengan software numerical taxonomy and multivariate analysis system (NTSYS) disimpulkan bahwa hubungan genetika antara kambing PE hasil kawin alam dengan inseminasi buatan adalah sebesar 48% dan dikategorikan rendah.
KOMPETENSI AKTIVASI PROTEIN EKSTRAK SPERMATOZOA PADA OOSIT M-II KAMBING BERDASARKAN ANALISIS PROFIL INTENSITAS KALSIUM (Ca+2) Gatot Ciptadi; Sri Rahayu; Budi Siswanto; Eva Ari Wahyuni; Aulanni’am A; Nur Ihsan
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.164 KB) | DOI: 10.21157/j.ked.hewan.v7i2.928

Abstract

Penelitian ini bertujuan mengkarakterisasi profil ekstrak spermatozoa (ES) dengan sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) elektroforesis dan mempelajari potensi ES dalam aktivasi M-II oosit. Kedua isolat protein total ES (2,5 µg/ml) serta protein spesifik 100 kD (1,98 µg/ml) disuplemensikan dalam medium aktivasi tissue culture medium (TCM)-199 stok. Intensitas Ca+2 diamati dengan fluo-3 menggunakan confocal laser scanning microscope (CLSM). Hasil penelitian menunjukkan bahwa suplementasi ES dan 100 kD protein menghasilkan oosit teraktivasi (cleavage) masing-masing 43,75 dan 2,00%. Intensitas Ca+2 menunjukkan adanya variasi dan pola yang berbeda, yakni intensitas fluoresen lebih tinggi pada oosit teraktivasi.
Phenotypic Similarities and Differences of Genetic Characteristic Boer and PE Goat Yield Crossbreeding 1st and 2nd Generation Rosidi Azis; Gatot Ciptadi; S. Suyadi
Chalaza Journal of Animal Husbandry Vol 5, No 2 (2020): Chalaza Journal of Animal Husbandry
Publisher : UNIVERSITAS SEMBILANBELAS NOVEMBER KOLAKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (968.76 KB) | DOI: 10.31327/chalaza.v5i2.1364

Abstract

This research aimed to analyze the phenotypic similarity and genetic characteristics of 1st and 2nd generation (G1 and G2) goat of Boer and PE goat yield crossbreeding. The number of offsprings of G1 and G2 was46 and 28 goats, respectively. Data analysis of phenotypic characteristics was estimated by calculating the opportunities of the number of the appearance of G1 and G2 offspring. The result showed that the percentages of the G2 of  W11 similarity (67.86%) more significant than G1 (26.09%).  The W21was the G1 (45.65%) higher than G2 (10.71%), and W31 was G1 (28.26%) higher than G2 (21.43%). It concluded that G2 had phenotypic similarities of the characteristic (color) in total progeny. The G2 goat displays the phenotypic characters of inheritance (color)patterns that had more significant similarity than the results of the G1, as well as similarities in morphological characters that were different from the G1. The characteristics (colors) in the G2 had dark brown head color greater than G1, and body conformation approaches to Boer goat. Other colors like light brown, plain white, black, and straight black on G1 greater than G2.
EVALUASI PERFORMA BOBOT BADAN DAN STATISTIK VITAL SAPI MADURA BERDASARKAN TAHUN KELAHIRAN Sulistyoningtyas I.; V.M. Ani Nurgiartiningsih; Gatot Ciptadi
JURNAL ILMIAH PETERNAKAN TERPADU Vol 5, No 2 (2017)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.063 KB) | DOI: 10.23960/jipt.v5i2.p40-43

Abstract

This research was done to compare performance of Madura cattle born in 2015 and 2016 at Unit of Technical for Animal Breeding and Animal Health Madura. Two hundred tails of Madura cattle that were one day old, 205 days old, and 365 days old were used in this research. They were offsprings of 12 tails of male cattle and 200 tails of female cattle. Variables observed were body weight, body height, body length, and heart girth. Data collected were analyzed by t test. Result of this research indicated that performance of Madura cattle that was one day old and 205 days old born in 2015 and in 2016 were not different (P>0.05). Performance of Madura cattle that was 365 days old born in 2015 and in 2016 were different (P<0.05). The performance of cattle born in 2015 versus in 2016 were 113.00±12.19 kg vs 120.00±10.86 kg for body weight (yearling weight),  110.00±6.81 cm vs 114.00±2.46 cm for body height, 103.00±6.70 cm vs 106.00±4.71 cm for body length, and 118.00±8.05 cm vs 120.00±5.91 cm for heart girth. It could be concluded that performance of Madura cattle that was 365 days old born in 2016 were higher than that was in 2015. Keywords: Madura cattle, Body weight, Bidy height, Body length, Heart girth
Environmentally Friendly Livestock Waste Management in High-Population Areas in Karang Ploso Malang Regency East Java Muhammad Arwani; Abdul Hakim; Agus Budiarto; Gatot Ciptadi; Yudit Oktanella
Jurnal Pengabdian Masyarakat Vol 4 No 2 (2023): Jurnal Pengabdian Masyarakat
Publisher : Institut Teknologi dan Bisnis Asia Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32815/jpm.v4i2.480

Abstract

Purpose: This abstract explores the need for integrated development in the agricultural and livestock sectors of Ngenep Village, Karangploso, Malang Regency, focusing on utilizing livestock waste effectively. Method: The study details the steps to develop a system for producing organic fertilizer and animal feed from agricultural and livestock waste. It highlights the successful implementation within the "Langgeng Mulya" dairy cattle farming community, emphasizing the utilization of waste for organic fertilizer in chili and tomato cultivation. Practical Applications: The research findings showcase the positive response of local farmers to the utilization of livestock waste, addressing the scarcity of organic fertilizer, particularly for tomato and chili growers. This sustainable approach fosters self-sufficiency and offers insights into waste-to-resource strategies. Conclusion: This study underscores the importance of integrating the agricultural and livestock sectors for sustainable waste management and resource utilization. It provides valuable lessons for enhancing the efficiency of rural development programs.
Co-Authors A, Aulanniam Abdul Hakim Abdul Rahem Faqih Achmad Iklilul L.L. As’ad Adelina Ari Hamiyanti Adelina Ari Hamiyanti Adriani Sunuddin Agung Pramana W.M Agung Pramana W.M Agus Budiarto Agus Budiarto Agus Budiarto Agus Budiarto Agus Susanto Akhiroh, Puji Ali Harris Andik Isdianto Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Apriani Herni Rophi Ardyah Ramadhina Irsanti Putri Ardyah Ramadhina Irsanti Putri Aris Subagyo Asma Naili Salsabila Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Aulia Puspita Anugra Yekti Azis, Rosidi Badriyah B Badriyah B, Badriyah Badrus Sholeh Bambang Semedi Bambang Susilo Bilqis Bilqis Brehaspati, Eka Wara Budi Siswanto Budi Siswanto Darmawan, Drajad Azis Dewi, Citra Satrya Utama Dian Ratnawati Diani, Khonita Rahma Doni Herviyanto Dyah Kinasih Wuragil Putu Raharjo Eko Wahyudi Enni Mutiati Eva Ari Wahyuni Eva Ari Wahyuni Fadillah Putra Fahmi, Mohammad Bachtiar Fitra, Ardian Hagus Tarno Harsuko Riniwati Hemiyanti, Adelina Ari Herandarudewi, Sekar Mira I.D. Retnaningtyas I.D. Retnaningtyas, I.D. Insafitri, I Irawati Dinasari Retnaningtyas Karim Khalifa Zamuna Khonita R. Diani Khonita Rahma Diani KIKI FIBRIANTO Kuswati Kuswati Laili Windah Fauziah Lilik Eka Radiati Liza Choirun Nisa M, Nur Ihsan M. Ruslin Anwar M.F. Wadjdi M.F. Wadjdi, M.F. Marjono Marjono Marjuki Marjuki Meyya L. Zain Mirsa Ita Dewi Moch. Aris Sunaryo Moch. Nasich Mohammad Bisri Mohammad Nur Ihsan Mubarak Z, Sultan Mudawamah Mudawamah Mudawamah Mudawamah Mudawamah Mudawamah Muhammad Arwani Muhammad Musa Muhammad Nur Ihsan Muhammad Nur Ihsan Muhammad Rizar Zakaria Muhammad Sumsanto Nail, Rory Abduh Nico Rahman Caesar Nuhfil Hanani Nur Ihsan Nur Ihsan Nurgiartiningsih, V. M. A. Nurhabib, Asro Nurul Jadid Mubarokati Nuryadi Nuryadi Putranto, Septiawan Putri, Ardyah Irsanti R. R. P. Putra Retnaningtyas, irawati Dinasari Ria Dewi Andriani Rizka Gitta Almaida Rizki Putra Samudra Roy Ardy Colas Napitupulu S. Chuzaemi S. Susilowati S. Susilowati Sambou, Omar Samudra, Rizki Putra Septi Anitasari, Septi Septiawan Putranto Setyawan, Hendrix Yulis Simbolon, Rosdiana Siska Nanda Widhaningrum Slamet Wahyudi Sony Susanto Sri Hindrawati Sri Rahayu Sri Rahayu Sri Rahayu SRI RAHAYU Sri Rahayu Sri Rahayu Sri Rahayu sri rahayu Sri Wahjuningsih Sucik Maylinda Sukandar Sukandar Sulistyoningtyas I. Sunaryo Sunaryo Sunaryo, Moch. Aris Sutiman B. Sumitro Sutiman Bambang Sumitro Sutopo, Bima Prakasa Dermawan Suyadi Suyadi Suyadi Suyadi Syafrizal Muhammad Syahrul Kurniawan Syamsul Arifin Teguh Wicaksono Tri Eko Susilorini Trinil Susilawati Trinil Susilawati V.M. Ani Nurgiartiningsih Wardani, Nezya Pramudya Wiadnya , Dewa Gede Raka Wike, Wike Wiwik Handayani Woro Busono, Woro Wresti Listu Anggayasti Wulandari, Eka Shinta Yanuhar, Uun Yudit Oktanella, Yudit Zulvado Satria Putra Yuwono