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Journal : journal of Basic Medical Veterinary

Identification of Cat (Felis catus) Blood Splatter on Cotton Fabric After Periods of Drying Using Leucomalachite Green and Takayama Reagent Charles, Vanessa Ann; Lestari, Tita Damayanti; Legowo, Djoko; Ismudiono, Ismudiono; Hidajati, Nove; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28593

Abstract

Blood-stain or blood splatter analysis when used properly can assist in establishing a chain of events linked to violent crimes (Bevel and Gardner, 2008). The methods used in detecting blood splatters in the field are chemical methods. Leucomalachite green is a colorimetric test which is used to test the presence of blood (Castro and Coyle, 2008). Takayama reagent is a confirmatory test for blood (Strassman, 1922). The aim of this research is to detect the blood splatter on cotton fabric after it has been dried for 1 day, 3 days and 5 days using Leucomalachite green and Takayama reagent. Cotton fabric was specifically chosen for this experiment with 3 different periods of drying. The unstained cotton fabric was cut into squares, and a blood sample was splattered on each piece. The fabrics splattered with blood were then dried for 1 day, 3 days and 5 days. The blood splatter was then tested using Leucomalachite green and Takayama reagent, and the results were noted afterwards. For the control, red food dye was dried for 1 day then tested with Leucomalachite green and Takayama reagent. The image results of the Leucomalachite green test are analyzed using ImageJ software 1.8.0_112 where the red, green and blue pixels are converted to grayscale. The image results of the Takayama test are graded based on the number and pattern of crystal. In conclusion, Leucomalachite green and Takayama reagent are able to detect cat blood splatter on the cotton fabric. Leucomalachite green produced a higher intensity/ darker colour as a result of an older sample, and the lower intensity/ lighter colour as a result of a fresher sample of the Leucomalachite green test. Takayama reagent produced a densely packed pattern of crystals as a result of an older sample, and the loosely packed pattern of crystals as a result of a fresher sample of the Takayama test.
Co-Authors Abdul Samik Adiana Mutamsari Witaningrum Al Arif, M Anam Al arif, Mohammad Anam Ali Agus Amrullah, Muhammad Fajar Annisa Lita Triagil Aryaloka, Suhita Ayuti, Siti Rani Belgania, Ristaqul Husna Berliana, Erprinanda Galuh Boedi Setiawan Budi Utomo BUDI UTOMO Budiarto Budiarto Budiarto Chandramohan, Koshini Charles, Vanessa Ann Chusniati, Sri Cindy Ercha Aulia Putri Dadik Rahardjo Dewi, Aprilia Kurnia Dikman, Dicky Mohammad Dimas Kunto Satrio Djoko Legowo Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri ERMA SAFITRI Faiqoh, Berliana Elok Farah Ghifara Hafidha Frida Aulya Arningdiah Gandul Atik Yuliani Gesa Cantya Primatara Hariadi, Mas'ud Hasib, Abdullah Heni Puspitasari Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Herupradoto, Eduardus Bimo Aksono Hidajati, Nove Hussain, Muhammad Asif Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Joel Jeevan Raj S/O Yogarajah Jola Rahmahani Khairullah, Aswin Rafif Kirana, Sabila Citra Kuncorojakti, Suryo Kurniawan, Muhammad ‘Ahdi Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mafruchati, Maslichah Mirni Lamid Mohammad Sofi’ul Anam Moses, Ikechukwu Benjamin Mufasirin Muhammad Al-Syafiq bin Abdul Halim Mustofa Helmi Effendi M’ Izi Kumala Lazuardi Sultoni Nanda Irsa Meydilasari Nove Hidajati Nuurin Ajrin Karim Pasang, Gabriel Sampe Permatasari, Dian Ayu Poedji Hastutiek Pristian Devon Martyas Wahyuningrum Pudji Srianto Putri, Natalie Lovena Pramono Raharjo, Dadik Raharjo, Hartanto Mulyo Rahmatullah, Aldin Akbar Rahmawati, Mitha Ardila Rahmi Sugihartuti Ratna Damayanti Rehman, Saifur Restiadi, Tjuk Imam Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Saputra, Rakan Mahiid Sari, Aulia Puspita Seagames Waluyo, Seagames Setiawan Koesdarto Shabira, Zahra Sharveihn Sathindren Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suharsono Suharsono Suherni Susilowati Sunaryo Hadi Warsito Supriyadi Supriyadi Supriyadi Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tri Wahyu Suprayogi Trilas Sardjito Viski Fitri Hendrawan Wahyuni, Retno Sri Warsito, Sunaryo Hadi Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wurlina, W Yulianna Puspitasari Yunus, Muchammad Zulfanisa, Rizky Asrin