Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

3.011

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Agrotrop : Journal on Agriculture Science Zuriat Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Aplikasi Bisnis dan Manajemen (JABM) E-Journal Widyariset Planta Tropika JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Biologi Tropis ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science JOURNAL OF SCIENCE AND SOCIAL RESEARCH axial : jurnal rekayasa dan manajemen konstruksi Menara Perkebunan Sainstek : Jurnal Sains dan Teknologi CORD Jurnal Penelitian Tanaman Industri (Littri) Buletin Palma Widyariset Journal Of Sustainability Perspectives Journal of Sustainability Perspectives Annales Bogorienses

Agus Purwito

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian IPB Jalan Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 350191

Aerospace Engineering Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

Published : 103 Documents Claim Missing Document

Claim Missing Document

Articles

4 5 6 7 8

Effect of Gamma Rays Irradiation and In Vitro Selection on Citrus nobilis (L.) ‘Siam Banyuwangi’ to Huanglongbing (HLB) Disease Dumaris Priskila Purba; Ali Husni; Alina Akhidaya; Mia Kosmiatin; Agus Purwito
AGRIVITA, Journal of Agricultural Science Vol 43, No 2 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v43i2.2887

Siam orange (Citrus nobilis L.) provides great economic value and social importance, despite its susceptibility to Huanglongbing (HLB) disease caused by Ca. Liberabacter sp., classified as specific bacterium phloem vessels marked by the formation of callouses covering plasmodesmata. This research aims to improve the tolerance of Siam orange from Banyuwangi (SB) to HLB disease. The experiment was undertaken by performing a randomized design (CRD) with one factor (irradiation dose). This study consists of two interrelated experiments, which include: induction of embryo mutations with gamma rays irradiation, and in vitro selection of putative mutant. The embryo of SB was irradiated by gamma rays with doses of 0, 45, 50, and 55 Gray. Each treatment was repeatedly undertaken for five times with 20 embryos. After 24 weeks, in vitro selection of putative mutant shoots was screened by HLB pathogen suspension. The observation indicated that treatment of gamma rays in various doses influenced embryo germination. In general, gamma ray irradiation gave significant effects on embryo germination and plant morphological characters. In vitro selection results for putative mutant on doses of 45, 50, and 55 indicated tolerance to HLB pathogen after selection.

Protoplast Fusion between Indonesian Citrus maxima (Burm.) Merr. and Citrus reticulata L.: A Preliminary Report Dyah Retno Wulandari; Agus Purwito; Slamet Susanto; Ali Husni; Tri Muji Ermayanti
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i0.950

Protoplast fusion is a useful technique for citrus genetic improvement. Establishment of protoplast technology could produce triploid seedles citrus and new citrus genetic combination. The aim of this research was to establish protoplast isolation, protoplast fusion between pummelo ‘Nambangan’ (Citrus maxima) and keprok ‘Garut’ (C. reticulata), and its protoplast culture. Protoplasts were isolated from leaves of in vitro seedlings of pummelo ‘Nambangan’ and embryogenic callus of keprok ‘Garut’ with two different compositions of enzymes. The results showed that optimum enzymes compositions for cells mesophyl wall degradation were consisted of 0.5 % Onozuka cellulase RS10, 0.5 % Macerozyme R10 and 0.1 % Pectolyase Y23. Optimum enzymes composition for callus embryogenic cells wall degradation were 0.5 % Onozuka cellulase RS10 added with 0.5 % Macerozyme R10. Protoplast fusion was conducted with PEG-mediated. Protoplast mixture were incubated in 40 % PEG for 8-10 min and after that could be observed 40.6 % of unfused protoplasts, 4.6 % of homofused mesophyl protoplasts, 28.2 % of homofused callus protoplasts, 7.8 % of heterofused and 9.6 % of multifused protoplasts from mesophyl and callus. Cells divided at the first week and began to form cell colonies afterwards in liquid culture.

PENYISIPAN GEN FITASE PADA TANAMAN TEBU cv. PA 175 MELALUI Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC) Susiyanti .; Gustav Adolf Wattimena; Memen Surahman; Agus Purwito; Dwi Andreas Santosa
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACT Sugarcane (Saccharum officinarum L.) is one of importance crop grown in marginal in Indonesia. Phosphorus (P) is critical to the growth and development of plant in the marginal land. P is stored in plant as phytic acid (myo-inositolhexakisphosphate). Phytic acid is hydrolyzed by the activity of phytases to yield inositol and free phosphate. Genetic transformation of sugarcane with phytases gene holds promise to provide enough P during period of rapid cell division and growth of plant. Plant transformation mediated by Agrobacterium tumefaciens, has become the most used method for the introduction foreign genes into plant cells and the sub sequens regeneration of transgenic plant. The selection and regeneration of embryogenic callus of transformed plant was done on MS medium containing kanamycin. The main objective of this study were: (1) To find the best kanamycin concentration for selectable marker; (2) Insert phytase gen into varieties of sugarcane (cv. PA 175) through Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC); and (3) To analyze of intregated transgene into genom of sugarcane using PCR method. Result of the experiment showed: (1) kanamycin selectable marker lethal doses for transformed sugarcane calli: 100 mg l-1; (2) Efficiency transformation of putative transgenic line was cv. PA 175= 24 %; (3) The first culture of transformed calli become 24 (Triton cv.), 18 (PSJT 94-41 cv.), and 30 (PA 175 cv.) putative plants; the second sub cullture of putative eksplant regenerate become new plant: 380 (PA 175 cv.) plants. (4) Analyzed of integrated phytase gene was proven by appearance of 900 bp of PCR band (5) transgenic plants (cv. PA 175) with highest activities respectively: 45 %; with medium phytase activity: 27 %, and low phytase activity: 27 % from total of sample. Non transgenic plants, most of sample show low phytase activity respectively: 100 % , noneh show medium - hight activy of phytase. Key words: Sugarcane, transformation, phytase, Agrobacterium tumefaciens

EFISIENSI MEDIA KULTUR DAN APLIKASI TEMPORARY IMMERSION SYSTEM PADA EMBRIOGENESIS SOMATIK KOPI ARABIKA / Efficiency of Culture Media and Aplication Temporary Immersion Systemon on Somatic Embryogenesis Arabica Coffee Meynarti Sari Dewi Ibrahim; RR. Sri Hartati; Rubiyo Rubiyo; Agus Purwito; Sudarsono Sudarsono
Jurnal Penelitian Tanaman Industri Vol 23, No 1 (2017): Juni, 2017
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

In vitro culture requires sucrose as carbon source and seaweed gel for condensing media. The price of sucrose and agar were quite expensive, causing difficulties in plant propagation using somatic embryogenesis technique. The purpose of this study was to examine the possibility to utilize sugar and commercial agar in somatic embryogenesis of Arabica coffee. The study was conducted in the Agricultural Superior Seed Development Unit, Indonesian Center Estate Crops Research and Development from May 2013 to June 2015.The first stage, calli were transferred into regeneration medium with tested added sucrose 35 g L-1+phytagel 2.5g L-1, and sugar 35 g L-1 +(phytagel 2.5 g L-1 or commercial agar 9 g L-1). In the second one, torpedo stage embryos transfered into media germination with examined sucrose 40 g L-1+ phytagel (2.5g L-1or 1.5g L-1), sugar40 g L-1 + (phytagel 2.5g L-1 or commercial agar 9 g L-1). The third stage,torpedos transferred into Temporary Immersion System (RITA), treatment examined sucrose and sugar.Experiments were arranged in completely randomized design with 10, 20 and 3 replication. The first stage, results showed sugar and commercial agar couldnot substitute sucrose and phytagel on regeneration media because it can reduce calli fresh weight and number of somatic embryos. The germination stage, sugar + phytagel (2.5 and 1.5 g L-1) can still be recommended, but not for sugar + commercial agar. Sugarin RITA may be used because had no significant effect on all parameters observed.Key words : Coffea arabika L., somatic embryogenesis, sugar, commercial agar, RITA. AbstrakPerkembangan dan pertumbuhan embriogenesis somatik memerlukan sukrosa sebagai sumber karbon, dan agar untuk memadatkan media. Harga sukrosa dan phytagel yang mahal merupakan kendala dalam perbanyakan tanaman menggunakan teknik embriogenesis somatik. Penelitian bertujuan untuk mengkaji kemungkinan penggunaan gula pasir dan agar komersial dalam embriogenesis somatik kopi Arabika. Penelitian dilakukan di Laboratorium Kultur Jaringan Tanaman, Unit Pengembangan Benih Unggul Pertanian, Badan Penelitian dan Pengembangan Pertanian Indonesia dari Mei 2013 sampai Juni 2015. Tahap pertama, kalus disubkultur pada media regenerasi. Perlakuan yang digunakan pemberian sukrosa 35 g L-1 + phytagel2,5 g L-1dan gula pasir 35 g L-1 + (phytagel 2,5 g L-1atau agar komersial 9 g L-1). Tahap kedua, embrio fase torpedo disubkultur pada media perkecambahan. Perlakuan yang digunakan pemberian sukrosa 40 g L-1 + phytagel (2,5 g L-1 atau 1,5 g L-1),dan gula pasir 40 g L-1 + (phytagel 2,5 g L-1atau agar komersial 9 g L-1). Tahap ketiga adalah subkultur torpedo ke Temporary Immersion System (RITA). Perlakuan yang digunakan adalah pemberian sukrosa dan gula pasir. Penelitian menggunakan rancangan acak lengkap dengan 10, 20 dan 3 ulangan. Hasil penelitian menunjukkan bahwa gula pasir dan agar komersial tidak dapat menggantikan sukrosa dan phytagel pada media regenerasi kalus kopi Arabika karena dapat menurunkan bobot basah kalus dan jumlah embrio somatik. Pada media perkecambahan pemberian gula pasir + phytagel (2,5 dan 1,5 g L-1) masih dapat direkomendasikan, tetapi tidak untuk penggunaan gula pasir + agar komersial. Pemakaian gula pasir pada RITA dapat digunakan karena tidak memberikan hasil yang berbeda nyata untuk semua peubah yang diamati.Kata kunci : Agar komersial, Coffea arabika L., embriogensis somatik, gula pasir, RITA

RESPON TANAMAN LADA (PIPER NIGRUML) VARIETAS CIINTEN TERHADAP IRADIASI SINAR GAMMA / Respons of Gamma Irradiation on Black Pepper (Piper nigrum L.) Ciinten Variety Nur Laela Wahyuni Meilawati; Nurliani Bermawie; Agus Purwito; Dyah Manohara
Jurnal Penelitian Tanaman Industri Vol 22, No 2 (2016): Juni, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n2.2016.71-80

Pepper is an introduced species and has always been propagated vegetatively, so it has narrow genetic base. High genetic diversity is necessary to produce new varieties, especially for breeding of resistance to foot rot disease. Increasing genetic diversity can be done through gamma ray irradiation. This research aims to evaluate response of black pepper Ciinten variety at seed and radicle emergence phases to gamma ray irradiation. The research was conducted in PAIR BATAN and greenhous e IMACRI from June 2014 to April 2015. The plant material was Ciinten variety at the seed and radicle emergence phases. The experimental design used was completely randomized design (CRD) with one factor which is dose of irradiation with seven levels (0, 25, 50, 75, 100, 125, 150) Gy. Each treatment consisted of three replications, each replication consisted of 60 seeds. Both phases showed significant differences in perfomances between dose in plant height, leaf length, number of leave, number of internode. Radiosensitivity of pepper on radicle emergence phase was higher than the seed phase indicated by LD50 (Lethal Dose 50). LD50 at seed phase was 68.15 Gy, whereas LD50 of the radicle emergence phase was 30 Gy. The higher irradiation dose that given to both treatment phases caused reduction in plant height, leaf length, while the number of leaves and nodes decreasing. Irradiation dose 25 dan 50 Gy in seed phase and 25 Gy in radicle emergence phase significantly increase genetic diversity base on quantitative, qualitative characters, anatomy and leaf resistence to P. capsici infection. Eighteen putative mutants resistant to infection P. capsici. Eighteen mutant putative Ciinten pepper varieties were expected to be high yielding varieties with more research in the greenhouse and in the field to determine the production and quality potential.

Penyebaran Polen Berdasarkan Analisis SSR Membuktikan Penyerbukan SITI HALIMAH LAREKENG; ISMAIL MASKROMO; AGUS PURWITO; NURHAYATI ANSHORI MATTJIK; SUDARSONO SUDARSONO
Buletin Palma Vol 16, No 1 (2015): Juni, 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v16n1.2015.77-92

ABSTRAK Analisis paternitas digunakan untuk mengetahui pola penyebaran serbuk sari pada kelapa (Cocos nucifera L.) tipe Dalam Kalianda. Tujuan penelitian ini adalah untuk (1) mengevaluasi pola penyebaran serbuk sari dan menentukan kisaran jarak penyebaran serbuk sari pada kelapa tipe Dalam Kalianda, (2) menentukan persentase penyerbukan silang (outcrossing) dan penyerbukan sendiri (selfing) yang terjadi pada kelapa tipe Dalam Kalianda, dan (3) menentukan frekuensi pola penyerbukan silang antara kelapa tipe Dalam normal (N) dengan kelapa Dalam Kopyor (K), KxN dan KxK yang terjadi pada populasi campuran antara kelapa tipe Dalam Kopyor dan kelapa Dalam normal Kalianda. Populasi yang digunakan terdiri atas 60 pohon kelapa tipe Dalam dewasa, 21 pohon merupakan kelapa tipe Dalam berbuah normal (homozigot KK) dan 39 merupakan pohon kelapa tipe Dalam Kopyor (heterosigot Kk). Empat belas pohon (5 pohon KK dan 9 pohon Kk) digunakan sebagai tetua betina. Sebanyak 49 progeni dipanen dari 15 induk terpilih dan dikecambahkan untuk sumber DNA dalam analisis paternitas. Enam lokus marka SSR polimorfik, yaitu CnCir_B12, CnCir_86, CnCir_87, CnCir_56, CnZ_51, CnZ_18 dan empat lokus marka SNAP polimorfik, yaitu CnSUS1#14,CnSUS1#3, CnWRKY6#3 dan CnWRKY19#1 digunakan untuk menentukan genotipe seluruh progeni, seluruh kandidat tetua jantan, dan semua tetua betina yang digunakan. Hasil penelitian menunjukkan bahwa serbuk sari kelapa tipe Dalam Kalianda menyebar dengan jarak terjauh 63 m. Jarak penyebaran serbuk sari terbanyak pada jarak 40-50 m, dengan frekuensi sebesar 13 kejadian polinasi (27%). Dari 47 progeni yang dievaluasi, hanya satu (2%) progeni yang berasal dari penyerbukan sendiri (self pollination) dan 48 (98%) berasal dari penyerbukan silang. Dari progeni hasil penyerbukan silang, 24 (49,0%) progeni teridentifikasi sebagai hasil persilangan antara induk dan tetua jantan kelapa tipe Dalam kopyor heterosigot Kk, 11 (22,4%) sebagai hasil persilangan antara induk kelapa tipe Dalam Kopyor heterosigot Kk dan normal homosigot KK, 10 (20,5%) sebagai hasil persilangan antara induk kelapa tipe Dalam normal homosigot KK dan Kopyor heterosigot Kk, serta 3 (6,1%) sebagai hasil persilangan antara induk dan tetua jantan tipe Dalam normal homosigot KK.Kata kunci : Kelapa Dalam Kopyor, kelapa Kopyor Kalianda, tingkat penyerbukan sendiri, tingkat penyerbukan silang. Pollen Dispersal Based on SSR Analysis Proves Kalianda to Kopyor Coconut PollinationsABSTRACT Paternity analysis was applied to determine the pattern of pollen spread among Kalianda Tall coconut (Cocos nucifera L.) in Kalianda, Lampung. The objectives of this research are to (1) evaluate patterns of pollen dispersal and ranges of pollen spread, (2) determine percentage of outcrossing or selfing rates, and (3) determine the frequency of cross pollination among normal (N) to kopyor (K), KxN and KxK in the mix population of Kalianda Tall coconut at Kalianda, Lampung. The population used in this study was 60 palms, consisted of 21 Kalianda Tall Normal coconuts (homozygous KK) and 39 Kalianda Tall Kopyor coconuts (Heterozygous Kk). Fourteen palms out of those were selected as female parents. Progeny arrays (49 nuts) were harvested from 15 female parents and they were germinated. The DNA was isolated from young leaf of all adult palms and germinated coconut seedlings and they were used in paternity analysis. Six polymorphic SSR marker loci used were CnCir_B12, CnCir_86, CnCir_87, CnCir_56, CnZ_51, CnZ_18 and the four polymorphic SNAP markers used were CnSUS1#14, CnSUS1#3, CnWRKY6#1 and CnWRKY19#3. The markers were used to genotype all the progenies, the potential male and the female parents. Results of the experiment indicated pollen of Kalianda Tall Kopyor coconut farthest disperse was 63 m. Distance of the mostpollen dispersal was between 40-50 m,with the frequency of 13 pollination events (27%). Among the evaluated progenies, only one (2%) comes from self pollination event and 48 (98%) comes from cross pollination. Results of the progeny evaluation also indicated 24 progenies (49.0%) are results of outcrossing among Kalianda Tall kopyor heterozygous Kk parents, 11 progenies (22.4%) are outcrossing among kopyor heterozygous Kk female and normal homozygous KK male parents, 10 progenies (20.5%) are outcrossing among normal homozygous KK female and kopyor heterozygous Kk male parents, and 3 progenies (6.1%) are outcrossing among normal homozygous KK female and male parents.Keywords : Tall kopyor coconut, Kalianda Kopyor coconut, self polination, cross pollination rate.

CHARACTERIZATION OF SOME MORPHOLOGY, ANATOMY AND PHYSIOLOGY OF THE WHEAT MUTANT (Triticum aestivum L.) DEWATA AND SELAYAR IN TROPICAL LOWLAND. Laela Sari; Agus Purwito; Didy Sopandie
Widyariset Vol 1, No 1 (2015): Widyariset
Publisher : Pusbindiklat - LIPI

Wheat mutant characterization is a part of the breeding programs to investigate the diversity that can influence in production increased. The aim of this research was to obtain data characterization morphology, anatomy and physiology that could be used as selection criteria and obtain an adaptive wheat mutant in the tropical lowland area. The research was conducted in Seameo-Biotrop Experimental Garden, located on ±250 m above the sea level, from April until November 2013. There were 18 Dewata wheat mutants and two Selayar wheat mutants, which is an M3 derivative resulted from EMS treatment, were used. LC50 Dewata got the 0.3% EMS treatment for 30 minutes, while LC50 Selayar got the 0.1% EMS treatment for 60 minutes. The data analysis used Augmented Designs Method. The results showed that the mutant Dewata that was significantly affected the morphology was indicated by four characters i.e. cooking time (two mutants), grain weight per genotype (seven mutants), leaf area (five mutants), and green leaves (one mutant). There are two characters in mutant Selayar that bring significant influence on morphological factors, those are seed weight per genotype (one mutant) and leaf area (one mutant), while the cooking time and green leaves have no real effect. Anatomical character appearance on leaf thickness and the size of stomata showed different levels of tolerance between Dewata plant mutant (DW-0,3.30-2-13-3), Selayar mutant (SL-0,1.60-2-14-2), and its both controls. As for the physiological character, there were significant differences in the amount of proline and glucose levels. Proline level in Dewata control was 79.29 µg/gBB, while in DW mutant -0,3.30-2-13-3 was 332.37 µg/gBB. Proline levels in Selayar control was 201.53 µg/gBB, while in mutant SL mutant-0,1.60-2-14-2 was 335.79µg/gBB. Likewise, glucose level in Dewata control was 14.32mg/gBB, while in DW mutant 0,3.30-1-15-1 was 29.06 mg/gBB, Selayar controls (5.87 mg /GBB) with SL mutant-0,1.60-2-14-2 (17.68 mg /gBB).

Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana Nurita TORUAN-MATHIUS; . LUKMAN; . AGUS-PURWITO
E-Journal Menara Perkebunan Vol 74, No 2: Desember 2006
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization for Cinchona plantlets from micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of C. ledgeriana QRC clone were used as scions, while of C. succirubra as rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization. Acclimatization consisted of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC for two weeks, followed by aclimatization in a plastic house with 70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan. Pada tanaman kina teknik sambung mikro in vitro belum pernah dilakukan. Tujuan penelitian ini adalah menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup, jumlah daun, berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung mikro yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro. Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur suhu 25 - 27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini 90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal Cinchona sp..

Kompatibilitas sambung mikro Cinchona ledgeriana dengan C. succirubra berdasarkan anatomi dan elektroforesis SDS- PAGE protein daerah pertautan Compatibility of micrografting Chincona ledgeriana and C. succirubra based on anatomy and SDS-PAGE protein electrophoresis of union area Nurita TORUAN-MATHIUS; . LUKMAN; . AGUS - PURWITO
E-Journal Menara Perkebunan Vol 75, No 2: Desember 2007
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

SummaryRootstocks and scions interaction causesdifferent responses within individuals of scionfrom the same clone. The objectives of thisresearch were to determine compatible anduncompatible combinations of micrograftingbetween Cinchona succirubra A, andC. succirubra B with C. ledgeriana QRC205and Cib5 clones, based on anatomy structureand SDS-PAGE protein bands pattern ofstem at union area between rootstock andscion. The research was arranged in aCompletely Randomized Design, withrootstock/scion combinations CSA/QRC205,CSA/Cib5, CSB/QRC205, CSB/Cib5 and as acontrols were the combination of CSA/CSA,CSB/CSB, QRC205/QRC205, Cib5/Cib5, succiand ledger seedlings with the same age. Effectof rootstocks on scion was studied based onanatomy structure of the union area betweenrootsocks and scions and SDS-PAGE proteinbands pattern. The results showed that theunion of stem between rootstocks and scionwas initiated by callus formation, cellsdifferentiation and the vascular vesselsformation. The anatomy of stem union area ofCSA/QRC205 as a compatible combination ofrootsotck and scion was the same asunmicrografted plantlet. At uncompatiblecombination CSB/Cib5 showed the formationof stone cells as a line along stem cyrcle atunion area and heavely callus formation atoutside of rootstock and scion stems. SDS-PAGE protein bands pattern from thecompatible combination was the same asplanlet control. On the otherhand, from theuncompatible combinations CSB/Cib5 werefound protein degradation and the formation ofnew proteins with molecular weight 21 and 30kD.RingkasanInteraksi batang bawah dengan batangatas menimbulkan berbagai keragaman responsantar individu batang atas dari klon yang sama.Tujuan penelitian ini adalah untuk me-netapkan kombinasi yang kompatibel denganyang tidak kompatibel antara kina klonCinchona succirubra A, dan C. succirubra Bdengan C. ledgeriana klon QRC205 danCib5, berdasarkan anatomi dan pola pitaSDS-PAGE protein daerah pertautan antarabatang atas dengan batang bawah. Percobaandisusun dengan Rancangan Acak Lengkapkombinasi yang diuji adalah CSA/QRC205,CSA/Cib5, CSB/QRC205, CSB/Cib5 dengankombinasi kontrol CSA/CSA, CSB/CSB,QRC205/QRC205, Cib5/Cib5, tanaman kinasucci dan ledger tanpa sambungan denganumur yang sama. Pengamatan dilakukanterhadap struktur anatomi daerah pertautanantar batang bawah dengan batang atas danpola pita protein SDS-PAGE batang atas. Hasilyang diperoleh menunjukkan bahwa tahapanpemulihan daerah pertautan penyambunganbatang bawah dengan batang atas diawalidengan pembentukan kalus, diferensiasi sel danterbentuknya jaringan ikatan pembuluhgabungan. Kombinasi antar batang bawahdengan batang atas yang kompatibel yaituCSA/QRC205 memperlihatkan strukturanatomi daerah pertautan yang serupa denganstruktur anatomi batang planlet yang tidakdisambung. Pada kombinasi yang tidak kom-patibel yaitu CSB/Cib5 pada daerah pertautanterbentuk sel-sel batu berbentuk garis yangmemanjang di tengah lingkaran batang. Disamping itu pada daerah pertautan terbentukkalus yang berlebih ke arah luar baik padabatang atas maupun batang bawah. Padakombinasi yang kompatibel pola pita proteinsama dengan planlet kontrol. Pada kombinasiyang tidak kompatibel yaitu kombinasiCSB/Cib5 terjadi degradasi protein danpembentukan protein baru dengan beratmolekul sekitar 21dan 30 kD

In Vitro Induction Of Tetraploid Pummelo ’Nambangan’ (Citrus maxima (Burm.) Merr.) By Colchicine Treatment Using Germinated Seed, Shoot Tip And Cotyledonary Node As Explants Dyah Retno Wulandari; Agus Purwito; Slamet Susanto; Ali Husni; Tri Muji Ermayanti
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Tetraploid citrus are important for interploidal hybridization to create triploid seedless citrus. Colchicine is the most commonly used as antimitotic agent to induce polyploid plants. Tetraploid induction by colchicine in Pummelo ‘Nambangan’ was conducted in vitro using different types of explants. The aim of this research was to induce tetraploid pummelo ‘Nambangan’ by colchicine treatment using germinated seed, shoot tip and cotyledonary node as explants. Tetraploid shoot induction was conducted by soaking germinated seeds, shoot tips and cotyledonary nodes in 0.1% colchicine for 1, 3 and 5 h. Regenerant shoots were grown on MS medium and their growth was observed after four weeks in culture. Ploidy level was determined using flow cytometry analysis. Stomata density, length and width of stomatal guard cell were also recorded. The results showed that shoot elongation was inhibited by colchicine treatment. Soaking of shoot tip explants in 0.1% colchicine for 1 h resulted in 66.66% of putative tetraploid shoots. Compared to diploid shoots, tetraploids had lower stomata density but bigger in guard cell size. Keywords: colchicine, tetraploid, pummelo (Citrus maxima (Burm.) Merr.), flow cytometry, stomata,

Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amrullah, Rizki Abi Anas D Susila Andri Ernawati Anjani, Imas Galuh Antonius Suwanto Arif Satria Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atra Romeida Awang Maharijaya Bambang S. Purwoko Bambang Sapta Purwoko Branco, Luis Manuel Budi Marwoto Budi Marwoto Budi Winarto Catur Herison Chaireni Martasari Dewi Sukma Dian Puji Rahayu Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih Endah Retno Palupi Endang Gunawan ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fathur Rahmi, Atika Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Harti, Heri Herison, Catur Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISKANDAR ZULKARNAEN SIREGAR ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Juang Gema Kartika Julius D. Nugroho Kalsum Yulifar, Andi Sri Ummi Kartiman, Roni Kartiman, Roni Karyanti ,, Karyanti Kikin H Mutaqin Komang Trisna Wirakusuma Kosasih, Akhmad KRISTIANTO NUGROHO, KRISTIANTO Laela Sari Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Maharijaya, dan Awang Marlin MASKROMO, ISMAIL Mastur Mastur Matjik, Nurhayati Anshori MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi MUHAMMAD HERMAN Muhammad Herman Muhammad Mahmud Muhammad Reza Zakie MUHAMMAD SYUKUR Ni Made Armini Wiendi Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie Nurul Khumaida Nurwita Dewi Prasetyawati , Adinda Prima Muklisa PUJI LESTARI Purba, Dumaris Priskila Putra, Heriansyah Putri, Halida Adistya RAGAPADMI PURNAMANINGSIH Ragapadmi Purnamaningsih Ratna Trisnawati Ravenska, Nidya Rd. Selvy Handayani Rd. Zainal Frihadian REFLINUR REFLINUR Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah Roedhy Poerwanto Roni Kartiman Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati RR. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo S. M. Sumaraow S. M. Sumaraw S. Sudarsono Sari, Dewi Citra Sari, Laela SATRIYAS ILYAS Sembiring, Rinawati SIENTJE MANDANG SUMARAW Sientje Mandang Sumaraw SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Slamet, Alim Setiawan Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono, dan SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO Sudirman Yahya Suhartanto , M. Rahmad Suhartanto, Muhammad Rahmad Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syaiful Anwar Syarifah Iis Aisyah TRI JOKO SANTOSO Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Warid Willy Bayuardi Suwarno Winarto, dan Budi Wulandari, Dyah Retno Wulandari, Dyah Retno Yopi Kurniawan

Title

Found 103 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 103 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 103 Documents
Search