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Efikasi Gen RB pada Tanaman Kentang Transgenik Katahdin SP904 dan SP951 terhadap Empat Isolat Phytophthora infestans dari Jawa Barat Ambarwati, Alberta Dinar; Sumaraw, S M; Purwito, Agus; Herman, Muhammad; Suryaningsih, E.; Aswidinnoor, Hajrial
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p28-36

Abstract

Efficacy of RB gene in transgenic potato Katahdin SP904and SP951 to West Java isolates of Phytophthorainfestans. A. Dinar Ambarwati, S.M. Sumaraw, AgusPurwito, M. Herman, E. Suryaningsih, and HajrialAswidinnoor. Potato late blight, caused by Phytophthorainfestans is one of the most devastating plant disease. Potatoyield losses due to this disease ranged from 47-100%. Amajor late blight resistance gene, called RB, previously wasidentified in the wild potato species Solanumbulbocastanum. RB gene has been integrated into cultivatedpotato Katahdin using Agrobacterium-mediated transformation,and showed durable and broad spectrum resistanceeither in laboratory assay or in confined field trial. Evaluationof transgenic Katahdin SP904 and SP951 was conducted toverify whether the RB gene with broad spectrum to allknown races of P. infestans in the United States and inToluca, Mexico was also effective against P. infestansisolates in Indonesia. Efficacy of RB gene was evaluated forfoliar and tuber resistance to West Java isolates. TransgenicKatahdin were more resistant in foliar than non transgenicplants, at 14 days after inoculation. Diseases intensity oftransgenic Katahdin SP904 and SP951 were 19.8-43.8%,whereas non transgenic Katahdin, Granola, and Atlanticwere 46.9-100%. In contrast to the foliar resistancephenotype, RB-containing tubers in transgenic Katahdin didnot exhibit increased resistance to Lembang, Pangalenganand Galunggung isolates. Tubers of transgenic KatahdinSP904, SP951, and non transgenic Katahdin showed lesionvolume of 0.93, 0.91, and 0.91 cm3, respectively. RB gene intransgenic Katahdin showed efficacy against late blight P.infestans in foliar, but did not showed efficacy in tuber.Transgenic Katahdin RB thus providing a potential source ofresistance for breeding programs.
Karakterisasi Morfologi, Anatomi dan Fisiologi Galur Mutan Gandum yang Ditanam di Dataran Rendah Tropik Sari, Laela; Purwito, Agus; Sopandie, Didy; Purnamaningsih, Ragapadmi; Sudarmonowati, Enny
Jurnal Penelitian Pertanian Tanaman Pangan Vol 35, No 1 (2016): April 2016
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.804 KB) | DOI: 10.21082/jpptp.v35n1.2016.p45-52

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Characterization of mutant wheat (Triticum aestivum L.) lines is a step on the breeding program to determine the beneficial characters for increasing the productivity in tropical lowland. The aim of this research was to obtain information on the variability of morphological, anatomical, and physiological characters that could be used as selection criteria and to obtain adaptive mutant lines of “Alibey” in tropical low altitude land. Research was conducted at the Experimental Farm of SEAMEO-BIOTROP in Bogor 250 m above sea level, from April to December 2013. Mutant lines of “Alibey” consisted of 16 M3 mutants resulted from treatments of EMS. LC50 of “Alibey” at 0.1% EMS for 60 minutes. Results showed that the mutant lines changed their morphological traits significantly, as indicated by the four characters i.e. long stem panicle (8 mutants), grain weight/panicle (1 mutant), weight of 100 seeds (4 mutants) and seed weight/plant (9 mutants). However, the mutant had no significant effect on the nine other characters, including: time of flowering, days to maturing, panicle length, plant height, number of tillers, panicle number, and leaf area. Anatomical characters namely leaf thickness and stomata size showed different values between “Alibey” mutant (AB-0.1.60-1-7-1) and the original Alibey. For the physiological characters there were significant differences among mutants with respect to the amount of proline and glucose levels. Proline level in the control plant was 4.15 ug/g BB, while that in mutant “AB-0.1.60-3-16-1” was 263.47 µg/g BB, and that in “AB-0.1.60-3-3-2” was 235.90 µ/g BB. Likewise, glucose level in control was 132.88 mg/ml, while in mutant “AB-0.1.60-3-16-1” was 181.48 mg/ml, and that in “AB-0.1.60-3-3-2” was 287.41 mg/ml. “Alibey” mutants should be selected based on two characters i.e. stem panicle length and seed weight/plant. Correlation analysis between panicle number and all other characters were not significant. Plant height significantly affected the grain weight/panicle and the grain weight/plant. It is expected that some of the mutants are adaptable to the tropical lowlands, so that the diversity of wheat germplasm in Indonesia is increased.
Pengaruh Umur Embrio dan Jenis Media Dasar Terhadap Keberhasilan Embryo Rescue Aren (Arenga pinnata (Wurmb) Merr.) secara In Vitro ARSYAD, MIRZA ARSIATY; SUDARSONO, nFn; PURWITO, AGUS; DINARTI, DINY
Buletin Palma Vol 14, No 1 (2013): Vol. 14 No. 1, Juni 2013
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v14n1.2013.20-27

Abstract

Tanaman aren (Arenga pinnata (Wurmb) Merr.) adalah salah satu jenis palma yang bernilai ekonomi tinggi. Salah satu aspek penting dalam pengembangan tanaman aren adalah penyediaan bibit. Embryo rescue diharapkan dapat menjadi alternatif solusi penyediaan bibit aren. Penelitian yang dilakukan bertujuan untuk 1) mengetahui pengaruh umur embrio zigotik terhadap perkecambahan serta perkembangan kecambah dalam embryo rescue aren, dan 2) untuk menguji efektifitas dua komposisi media dasar yang digunakan dalam kultur embrio zigotik aren secara in vitro. Penelitian ini dilaksanakan di Laboratorium Biologi dan Molekuler Tanaman, IPB dari Oktober 2011 sampai Oktober 2012. Penelitian disusun dalam bentuk percobaan faktorial menggunakan Rancangan Acak Lengkap (RAL) dengan lima ulangan. Embrio zigotik yang berasal dari buah aren muda (±15 bulan) dan tua (±30 bulan) dikultur pada media dasar Y3 dan WPM tanpa penambahan zat pengatur tumbuh (ZPT). Hasil yang diperoleh menunjukkan bahwa kemampuan hidup dan perkecambahan embrio zigotik aren terbaik diperoleh pada eksplan embrio muda (92%) dibandingkan embrio tua (72%). Komposisi media dasar (Y3 dan WPM) tidak berpengaruh nyata terhadap perkecambangan embrio aren. Haustorium dan apokol dalam kultur in vitroada yang berkembang menjadi bentuk-bentuk abnormal dengan persentase hingga 32% untuk haustorium dan 26% untuk apokol. Perkembangan haustorium dan apokol abnormal tidak berpengaruh terhadap planlet yang terbentuk dari embrio zigotik. Persentase planlet yang terbentuk dari embrio zigotik aren berkisar 6%-25% pada umur embrio zigotik dan jenis media yang diuji. Kata kunci : Aren, embrio muda, embrio tua, WPM, Y3.ABSTRACTInfluence of Embryo Age and Type of Basal Mediumto The Success of In Vitro Embryo Rescue of Sugar Palm (Arenga pinnata (Wurmb) Merr.)Sugar palm is one of the palms that has high economic value. Provision of seedling is one of important aspect to develop plant, include sugar palm (Arenga pinnata (Wurmb) Merr.) for cultivation and breeding program. Embryo rescueis expected tobe an alternative solution on provision of sugar palm seedlings. The objectives of the experiment were 1) to evaluate the effect of zygotic embryo age on germination and development of sugar palm rescued zygotic embryoand 2) to evaluate the effect of basal medium composition on sugar palm embryo culture. The experiment was conducted at Plant Molecular and Biology Laboratory of IPB, from October 2011 to October 2012. The experiment was compiled in the form of factorial experiment using Completely Randomized Design (CRD) with five replications. Zygotic embryos derived from immature (± 15months) and mature sugar palm fruit (± 30 months) were cultured on Y3 and WPM mediumin absence of plant growth regulators. The result showed that the best viability and germination of zygotic embryo obtained on immature zygotic embryo (92%) compare to the mature embryos (72%). The composition of the basal medium (Y3 and WPM) did not significantly affect on embryo germination. There was evidence that haustorium and cotyledonary petiole in in vitro culture that grown into abnormal shapes with percentages up to 32% and 26% respectively. Abnormality of haustorium and cotyledonary petiole development did not affect on plantlets derived zygotic embryos. Percentage of plantlets obtained from zygotic embryos was 6%-25% at the age ofzygotic embryo and types of basal medium that were tested.
Morphological Characteristics of Ectomycorrhizas on Merbau [Intsia bijuga (Colebr.) O. Kuntze] JULIUS DWI NUGROHO; IRDIKA MANSUR; AGUS PURWITO; ENDANG SUHENDANG
HAYATI Journal of Biosciences Vol. 17 No. 2 (2010): June 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.072 KB) | DOI: 10.4308/hjb.17.2.68

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Merbau [Intsia bijuga (Colebr.) O. Kuntze] is one of valuable timber tree in south-east Asia which has been known having ectomycorrhizae (EcM) though being ignored. Identification of the ectomycorrizae is prime important for being basis of further studies. This study investigated the EcM fungi associated with Merbau by using both sporocarp morphology and EcM morphotypes. Morphological characters of sporocarps and basidiocarps of the fungi and EcM morphotypes obtained from seedlings and trees from natural and plantation stands of merbau, as well as from nurseries were compared to the description of those resulted from baiting method. Only one species of ectomycorrhizal fungus was found associated with merbau [Intsia bijuga (Colebr.) Kuntze] which has not been described yet. The fungus formed mycorrhizae with monopodial pinnate branching. The fungus was identified belonging to the genus of Scleroderma. The fungus was more common to occur beneath merbau seedlings than trees. The sporocarps may be yielded under greenhouse condition and could be cultured in MMN agar media, thus it facilitates to the production of inoculums used for further studies.
Variasi Genetik Mutan Anggrek Spathoglottis plicataBlume. Berdasarkan Marker ISSR Atra Romeida; Surjono Hadi Sutjahjo; Agus Purwito; Dewi Sukma; , Rustikawati
Indonesian Journal of Agronomy Vol. 40 No. 3 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.864 KB) | DOI: 10.24831/jai.v40i3.6829

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Mutants of Spathoglottis plicataBlume were obtained from plants treated with Gamma Irradiation at 30-100 Gray. The mutants showed variations in the flower morphology. The objective of this experiment was to identify genetic variations of orchids S. plicataand its mutants using inter-simple sequent repeat (ISSR) markers. The amplified product of 10 ISSR primers produced 360 bands and 71 ISSR of the loci (90.14%) were polymorphic. The coefficient of similarity and principal component analysis produced five major groups with similarity coefficient of 0.68. The goodness of fit correlation matrix value reached 0.91. Therefore the ISSR isa good marker for identification of S. plicatamutants.Keywords: gamma irradiation, ISSR marker, mutant, orchid
Induksi Kalus dan Embrio Somatik Tanaman Jambu Biji Merah (Psidium guajava L.) Reza Ramdan Rivai; Ali Husni; Agus Purwito
Buletin Agrohorti Vol. 2 No. 1 (2014): Januari 2014
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.693 KB) | DOI: 10.29244/agrob.2.1.49-58

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Penelitian ini bertujuan untuk memperoleh protokol yang tepat dalam menginduksi kalus dan embrio somatik tanaman jambu biji merah.  Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan, Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor pada bulan September 2012 sampai dengan bulan Maret 2013. Penelitian tersusun atas dua percobaan yaitu percobaan pertama merupakan induksi kalus dan percobaan kedua merupakan induksi embrio somatik. Hasil penelitian menunjukkan bahwa media dengan perlakuan zat pengatur tumbuh R1 (5 mg l-1 2.4-D), R3 (5 mg l-1 2.4-D + 1 mg l-1 BAP) dan R6 (2.5 mg l-1 2.4-D + 2 mg l-1 2-iP) merupakan perlakuan terbaik untuk menginduksi kalus kompak pada 4 minggu setelah tanam (MST), sedangkan untuk menginduksi kalus remah didapatkan media dengan perlakuan zat pengatur tumbuh terbaik yaitu R5 (5 mg l-1 2.4-D + 2 mg l-1 2-iP). Embrio somatik primer terbentuk pada perlakuan R2E4 (2.5 mg l-1 2.4-D dipindahkan ke 1 mg l-1 2.4-D + 2 mg l-1 2-iP) dan perlakuan R5E4 (5 mg l-1 2.4-D + 2 mg l-1 2-iP dipindahkan ke 1 mg l-1 2.4-D + 2 mg l-1 2-iP) masing-masing pada 12 dan 16 MST.
Keragaan In Vitro dan In Vivo Hibrida Somatik antara Solanum melongena cv. Dourga dengan Solanum torvum Sw. Agus Joko Santoso; Ali Husni; Agus Purwito
Buletin Agrohorti Vol. 1 No. 4 (2013): Oktober 2013
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.092 KB) | DOI: 10.29244/agrob.1.4.65-74

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Terung adalah tanaman yang mudah terserang oleh banyak jenis penyakit. Salah satu usaha yang dapat dilakukan untuk meningkatkan ketahanan terhadap penyakit tersebutadalah dengan mengintroduksi sifat ketahanandari kerabat liarnya melalui fusi protopas. Penelitian ini dilakukan untuk mempelajari keragaan in vitro dan in vivo hibrida somatik hasil fusi protoplas Solanum melongena cv. Dourga dengan Solanum torvum Sw.  Penelitian ini merupakan penelitian faktor tunggal dengan tujuh taraf klon, yaitu Solanum melongena cv. Dourga (SM), Solanum torvum Sw. (ST), dan 5 klon hibrida somatik (SMST1,  SMST2, SMST3, SMST4, and SMST5) yang disusun menggunakan Rancangan Acak Lengkap (RAL). Peubah yang diamati antara lain tinggi tanaman, jumlah daun, panjang daun, panjang tangkai daun, jumlah akar, panjang akar, jumlah buku, warna batang, warna tangkai daun, jumlah duri pada permukaan daun, dan bentuk lobus daun. Pada kondisi in vitro, pertumbuhan Solanum torvum Sw. berbeda sangat nyata dengan kelima klon hibrida somatik. Klon SMST1, SMST2, dan SMST4 menunjukkan pertumbuhan yang lebih baik  dari klon SMST3 dan SMST5. Pada kondisi in vivo, klon-klon yang diuji memiliki kekerabatan yang lebih dekat dengan Solanum torvum Sw.
Kombinasi persilangan dan seleksi in vitro untuk mendapatkan kultivar unggul kentang Agus Purwito; GA Wattimena
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 3 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (720.108 KB)

Abstract

Cultivars Granola  dan Atlantic are the best cultivars so far for farmers, due to it adaptation in Indonesia. Cv Granola is resistant to several important diseases, but the quality is low, thus only produce for vegetable purposes. Cv Atlantic is the best cultivar, in term of productivity and quality. Cv Atlantic is suitable for industry purposes, susceptible to several important diseases. Crossing between both cultivars expected to produce high yielding cultivars and adapted to Indonesian environment. The method used was crossing and germinated the seed then selected in vitro. Each germinated seed was propagated in vitro and considered as clones. In vitro selection was performed through vigour, bacterial wilt and soft rot test, as well as micro tuber production. The result of selectionwas then verified in the field. From thousands of seed germinated producing 12 selected clones, 7 clones of them were showed better then their parent in term of tuberization and micro tuber production. The clones were Atnola 1, Atnola 5, Atnola 3, Atnola 10, Atnola 12, Atnola 24, and Atnola 26. Four clones showed level of resistance better than the parent, namely Atnola 3, Atnola 5, Atnola 8, and Atnola 10. The result of field test showed that the seven clones produced better tuber than the parent Atlantic dan Granola, which were Atnola 5 dan Atnola 10 showed vigour, tuber weight, and better level of resistance, thus will be the candidate of high yielding cultivars.
Pembentukan Umbi Lapis Mikro Dua Kultivar Bawang Merah (Allium cepa var. Aggregatum Group) pada Beberapa Konsentrasi Succunic Acid Daminozide Hydrazide Dini Dinarti; Agus Purwito; Anas D Susila; Iis Rahmawati
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 1 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.205 KB)

Abstract

The objective of this research was to study shallot bulb formation on few concentrations of growth retardant succinic acid daminozid hydrazide (SAOH). Completely Randomized Design with 2 factors were used in this experiment. The first factor was four concentrations of SAOH (0, 30, 60 and 90 ppm) and second was two cultivars of shallot (Bima Juna and Kuning Tablet). The cultivars did not give significant effect to total number of leaf, shoot, root, number and weight of bulb, diameter of bulb, and height of plantlets. While SADH concentrations gave very significant effect to number of leaf, but not significant to number of root, number and weight of bulb, diameter of bulb and height of plantlets. Combinations of the two factors only gave significant effect to number of leaf and shoot but not significant to number of root, number and weight of bulb and height of plantlets.   Keywords: bulb formation, SAOH, shallot
The In Vitro Screening of Drought Tolerant Potatoes (Solanum tuberosum L) of the Center for Tropical Horticulture Studies - IPB collections Jane Kathryne Jolanda Laisina; Awang Maharijaya; Sobir Sobir; Agus Purwito
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 2 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.2.235

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In supporting the replacement of potato varieties with the varieties that are adaptive to drought, effective and fast methods are needed. One of which is through in vitro screening of drought-tolerant genotypes. The aims of this research were to determine the appropriate concentration of sorbitol as a critical limit in the in vitro screening process of drought-tolerant genotypes, as well as to determine vegetative characteristics that could be used as indicators of in vitro selection and also to obtain in vitro drought-tolerant genotypes. To achieve these objectives, we used single-nodal explants of seven genotypes cultured for six weeks on MS media added with four levels of sorbitol concentration. Of the four treatments, sorbitol level that could distinguish susceptible and tolerant plants was 0.2 M sorbitol and at this concentration, the characteristics that showed significant interactions were plant height and root length, so that both characteristics could be used as selection indicators for the analysis of tolerance tests for the seven genotypes. The results of tolerance test using the analysis of relative decline and stress-susceptibility index (SSI) showed that PKHT4 and PKHT6 were the genotypes that were tolerant in vitro. Keywords: drought tolerant, in vitro, Solanum tuberosum, sorbitol
Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amrullah, Rizki Abi Anas D Susila Andri Ernawati Anjani, Imas Galuh Antonius Suwanto Arif Satria Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atra Romeida Awang Maharijaya Bambang S. Purwoko Bambang Sapta Purwoko Branco, Luis Manuel Budi Marwoto Budi Marwoto Budi Winarto Catur Herison Chaireni Martasari Dewi Sukma Dian Puji Rahayu Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih Endah Retno Palupi Endang Gunawan ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fathur Rahmi, Atika Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Harti, Heri Herison, Catur Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISKANDAR ZULKARNAEN SIREGAR ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Juang Gema Kartika Julius D. Nugroho Kalsum Yulifar, Andi Sri Ummi Kartiman, Roni Kartiman, Roni Karyanti ,, Karyanti Kikin H Mutaqin Komang Trisna Wirakusuma Kosasih, Akhmad KRISTIANTO NUGROHO, KRISTIANTO Laela Sari Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Maharijaya, dan Awang Marlin MASKROMO, ISMAIL Mastur Mastur Matjik, Nurhayati Anshori MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi Muhammad Herman MUHAMMAD HERMAN Muhammad Mahmud Muhammad Reza Zakie MUHAMMAD SYUKUR Ni Made Armini Wiendi Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie Nurul Khumaida Nurwita Dewi Prasetyawati , Adinda Prima Muklisa PUJI LESTARI Purba, Dumaris Priskila Putra, Heriansyah Putri, Halida Adistya Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Trisnawati Ravenska, Nidya Rd. Selvy Handayani Rd. Zainal Frihadian REFLINUR REFLINUR Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah Roedhy Poerwanto Roni Kartiman Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri RR. Sri Hartati Rr. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo S. M. Sumaraow S. M. Sumaraw S. Sudarsono Sari, Dewi Citra Sari, Laela SATRIYAS ILYAS Sembiring, Rinawati Sientje Mandang Sumaraw SIENTJE MANDANG SUMARAW SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Slamet, Alim Setiawan Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono, dan SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO Sudirman Yahya Suhartanto , M. Rahmad Suhartanto, Muhammad Rahmad Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syaiful Anwar Syarifah Iis Aisyah TRI JOKO SANTOSO Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Warid Willy Bayuardi Suwarno Winarto, dan Budi Wulandari, Dyah Retno Wulandari, Dyah Retno Yopi Kurniawan