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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Agrotrop : Journal on Agriculture Science Zuriat Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Aplikasi Bisnis dan Manajemen (JABM) E-Journal Widyariset Planta Tropika JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Biologi Tropis ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science JOURNAL OF SCIENCE AND SOCIAL RESEARCH axial : jurnal rekayasa dan manajemen konstruksi Menara Perkebunan Sainstek : Jurnal Sains dan Teknologi CORD Jurnal Penelitian Tanaman Industri (Littri) Buletin Palma Widyariset Journal Of Sustainability Perspectives Journal of Sustainability Perspectives Annales Bogorienses

Agus Purwito

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian IPB Jalan Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 350191

Aerospace Engineering Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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PERBAIKAN KUALITAS BERKELANJUTAN PENYELENGGARAAN AKADEMIK PROGRAM SARJANA ILMU KOMPUTER FMIPA IPB Rd. Zainal Frihadian; Agus Purwito; Imas Sukaesih Sitanggang
Jurnal Aplikasi Bisnis dan Manajemen (JABM) Vol. 2 No. 2 (2016): JABM Vol. 2 No. 2, Mei 2016
Publisher : School of Business, Bogor Agricultural University (SB-IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17358/jabm.2.2.207

The quality of a university is not only measured by its academic program inputs but also by its processes, outputs, and outcomes. Performance measurement can be conducted by looking at its achievements at a certain period of time compared with its targets and baseline. It is a fact that the academic implementation of the Undergraduate Program of Computer Science of Faculty of Mathematics and Natural Science IPB is unsatisfactory viewed from the average GPA and study duration during the academic years of 2009-2013. This study aims to to describe the quality of the implementation of the Undergraduate Program of Computer Science and the efforts made by the stakeholders in maintaining and improving the quality and to formulate alternative strategies to obtain better quality. Fishbone analysis method was used to determine the cause of an unsatisfactory average GPA and longer study duration, and descriptive analysis was used to describe the academic quality of the undergraduate program of Computer Science. The results showed the academic quality of the academic year of 2009-2013 has not reached the targets set in the strategic plan, but if seen from the figures, its accomplishments have nearly reached the targets; therefore, it can be said the quality is reasonably good. Constructive efforts in running the academic administration of the study program have also been implemented by the management.Keywords: academic quality, period of study, quality improvement, FMIPA IPB

PENCAPAIAN INDIKATOR KINERJA BIDANG AKADEMIK PROGRAM SARJANA PADA FAKULTAS PERIKANAN DAN ILMU KELAUTAN INSTITUT PERTANIAN BOGOR DALAM PERSPEKTIF PENJAMINAN MUTU Yopi Kurniawan; Agus Purwito; Tri Wiji Nurani
Jurnal Aplikasi Bisnis dan Manajemen (JABM) Vol. 2 No. 3 (2016): JABM Vol. 2 No. 3, September 2016
Publisher : School of Business, Bogor Agricultural University (SB-IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17358/jabm.2.3.258

Faculty of Fisheries and Marine Science, Bogor Agricultural University, has been running a sustainable quality assurance system in both academic and non-academic fields. The success of academic management can be examined through the achievement of established performance indicators. This study aimed to analyze the performance of undergraduate academic program in the period of 2005—2013. Thus, it also aims to investigate the causes of the decline in the achievement of academic performance indicator. The results showed that the achievement of FPIK performance indicator in 2013 was 81.45%. This value decreased compared to that in 2012 i.e. 85.09%. The achievement of academic performance indicators of undergraduate program in 2013 was 7.99%, decreasing from the previous year of 11.2%. For the past decades, FPIK has put efforts to improve its achievement of performance indicator in the perspective of quality assurance. By referring to SWOT analysis, strategies are formulated to improve the qualities of input, process and output. To ensure the continuous improvement of qualities, quality assurance strategy is adopted.Keywords: performance indicator, quality assurance, continuous improvementABSTRAKFakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, telah menjalankan sistem penjaminan mutu yang berkelanjutan dalam bidang akademik dan non akademik. Keberhasilan pengelolaan bidang akademik dapat dilihat pada pencapaian indikator kinerja yang telah ditentukan. Dalam penelitian ini, bertujuan untuk menganalisis pencapaian kinerja bidang akademik program sarjana pada periode 2005–2013. Dalam penelitian ini juga bertujuan mempelajari penyebab penurunan pencapaian indikator kinerja bidang akademik. Hasil penelitian menunjukkan bahwa pencapaian indikator kinerja FPIK tahun 2013 sebesar 81,45%. Nilai ini menurun dibandingkan dengan pencapaian kinerja tahun 2012 sebesar 85,09%. Pencapaian indikator kinerja bidang akademik program sarjana pada tahun 2013 sebesar 7,99%, nilai ini turun dari tahun sebelumnya sebesar 11,2%. FPIK telah melakukan banyak hal untuk meningkatkan pencapaian indikator kinerja dalam perspektif penjaminan mutu untuk dekade terakhir. Dengan analisis SWOT dihasilkan strategi untuk peningkatan kualitas input, proses dan output. Untuk menjamin perbaikan perbaikan mutu yang berkelanjutan digunakan strategi penjaminan mutu. Kata kunci: indikator kinerja, penjaminan mutu, perbaikan berkelanjutan

CHARACTERIZATION OF SOME MORPHOLOGY, ANATOMY AND PHYSIOLOGY OF THE WHEAT MUTANT (Triticum aestivum L.) DEWATA AND SELAYAR IN TROPICAL LOWLAND. Laela Sari; Agus Purwito; Didy Sopandie
Widyariset Vol 1, No 1 (2015): Widyariset
Publisher : Pusbindiklat - LIPI

Wheat mutant characterization is a part of the breeding programs to investigate the diversity that can influence in production increased. The aim of this research was to obtain data characterization morphology, anatomy and physiology that could be used as selection criteria and obtain an adaptive wheat mutant in the tropical lowland area. The research was conducted in Seameo-Biotrop Experimental Garden, located on ±250 m above the sea level, from April until November 2013. There were 18 Dewata wheat mutants and two Selayar wheat mutants, which is an M3 derivative resulted from EMS treatment, were used. LC50 Dewata got the 0.3% EMS treatment for 30 minutes, while LC50 Selayar got the 0.1% EMS treatment for 60 minutes. The data analysis used Augmented Designs Method. The results showed that the mutant Dewata that was significantly affected the morphology was indicated by four characters i.e. cooking time (two mutants), grain weight per genotype (seven mutants), leaf area (five mutants), and green leaves (one mutant). There are two characters in mutant Selayar that bring significant influence on morphological factors, those are seed weight per genotype (one mutant) and leaf area (one mutant), while the cooking time and green leaves have no real effect. Anatomical character appearance on leaf thickness and the size of stomata showed different levels of tolerance between Dewata plant mutant (DW-0,3.30-2-13-3), Selayar mutant (SL-0,1.60-2-14-2), and its both controls. As for the physiological character, there were significant differences in the amount of proline and glucose levels. Proline level in Dewata control was 79.29 µg/gBB, while in DW mutant -0,3.30-2-13-3 was 332.37 µg/gBB. Proline levels in Selayar control was 201.53 µg/gBB, while in mutant SL mutant-0,1.60-2-14-2 was 335.79µg/gBB. Likewise, glucose level in Dewata control was 14.32mg/gBB, while in DW mutant 0,3.30-1-15-1 was 29.06 mg/gBB, Selayar controls (5.87 mg /GBB) with SL mutant-0,1.60-2-14-2 (17.68 mg /gBB).

RESISTANCE EVALUATION ON POPULATIONS OF CROSSES BETWEEN TRANSGENIC POTATO KATAHDIN RB AND NON-TRANSGENIC ATLANTIC AND GRANOLA TO LATE BLIGHT (Phytophthora infestans) IN CONFINED FIELD TRIAL Alberta Dinar Ambarwati; Muhammad Herman; Agus Purwito; Sientje Mandang Sumaraw; Hajrial Aswidinnoor
Indonesian Journal of Agricultural Science Vol 12, No 1 (2011): April 2011
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v12n1.2011.p33-39

Late blight resistance gene (RB gene) isolated from Solanum bulbocastanum, is a broad resistance gene against all races of Phytophthora infestans. The gene was transformed into Katah-din event SP904 and SP951 using Agrobacterium tumefaciens and these transgenic plants have been crossed with susceptible potato cultivars Atlantic and Granola. Populations of the crosses have been molecularly characterized for the integration of the RB transgene. The study aimed to evaluate the resistance of the populations of crosses between transgenic Katahdin RB and susceptible non-transgenic parents (Atlantic and Granola) to late blight in a confined field trial at Pasir Sarongge, Cianjur, West Java. A total of 84 clones originated from four popula-tions were evaluated for resistance to late blight. These included 22 clones of Atlantic x transgenic Katahdin SP904, 16 clones of Atlantic x transgenic Katahdin SP951, 19 clones of Granola x transgenic Katahdin SP904, and 27 clones of Granola x transgenic Katahdin SP951. Observations of the late blight infection were conducted when late blight symptoms were detected, i.e. at 56, 60, 63, 70, and 77 days after planting (DAP). The result showed there were high variations in the resistance level of all the 84 clones tested. Clones of crosses between susceptible parents (Atlantic or Granola) and resistant parents (transgenic Katahdin SP904 or Katahdin SP951) showed a similar pattern based on the area under disease progress curve (AUDPC) value, i.e. 377.2 greater than the AUDPC of the resistant parents (180.1), but smaller than that of the susceptible parents (670.7). Observation at 77 DAP resulted four resistant potato clones having resistance score of 7.0-7.6, higher than the transgenic parents Katahdin SP904 (4.6) and Katahdin SP951 (6.8), i.e. clone B8 (Atlantic x transgenic Katahdin SP951) with resistance score of 7.6 and clones B26 (Atlantic x transgenic Katahdin SP951), C183 (Granola x transgenic Katahdin SP904), and D89 (Granola x transgenic Katahdin SP951) with resistance score of 7. These four transgenic potato resistant clones need to be further developed as promising potato clones to late blight.

DIRECT AND INDIRECT SOMATIC EMBRYOGENESIS ON ARABICA COFFEE (Coffea arabica) Meynarti Sari Dewi Ibrahim; Rr. Sri Hartati; Rubiyo Rubiyo; Agus Purwito; Sudarsono Sudarsono
Indonesian Journal of Agricultural Science Vol 14, No 2 (2013): October 2013
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v14n2.2013.p79-86

Propagation of Coffea arabica L. through direct and indirect somatic embryogenesis technique is promising for producing large number of coffee seedlings. The objectives of the research were to evaluate methods for direct and indirect somatic embryo-genesis induction of C. arabica var. Kartika. The explants were the youngest fully expanded leaves of arabica coffee. The evalu-ated medium was modified Murashige and Skoog (MS) medium supplemented with a combination of 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; 4.52 µM 2,4-D + 4.54 or 9.08 µM thidiazuron; or 9.04 µM 2,4-D + 9.08 µM thidiazuron. Both calli (100 mg) and pre-embryos developed from the edge of leaf explants were subcultured into regeneration medium (half strength MS with modified vitamin, supplemented with kinetine 9.30 µM and adenine sulfate 40 mg L-1). The results showed coffee leaf explant cultured on medium containing 2.26 µM 2,4-D + 4.54 or 9.08 µM thidiazuron to induce direct somatic embriogenesis from explant, while that of 4.52 or 9.04 µM 2,4-D + 9.08 µM thidiazuron to induced indirect somatic embrio-genesis. The medium for calli induction from coffee by explants was medium supplemented with 4.52 or 9.04 µM 2,4-D in combination with 9.08 µM thidiazuron. On the other hand, the best medium for activation of induction of somatic embryos was MS medium supplemented with 9.04 µM 2,4-D + 9.08 µM thidiazuron. Based on this results, the first step for developing micropropagation for coffee has been resolved. The subsequent studies will be directed to evaluate agronomic performance of the derived planting materials.

Pengaruh Iradiasi Sinar Gamma pada Generasi Pertama (M1) untuk Mendapatkan Genotipe Unggul Baru Kedelai Toleran Kekeringan WARID WARID; NURUL KHUMAIDA; AGUS PURWITO; MUHAMMAD SYUKUR
Agrotrop : Journal on Agriculture Science Vol 7 No 1 (2017)
Publisher : Fakultas Pertanian Universitas Udayana

Influence of Gamma Rays Irradiation on First Generation (M1) to Obtain New Promising Drought-Tolerance Soybean Genotype. Breeding to produce drought tolerant varieties is one of main strategies in the soybean production improvement. Some limitations in soybean conventional breeding are that it takes long time and that drought tolerant characters are rarely found. Therefore, the improvement of genetic variability is very important to assist drought tolerant. Mutation through gamma irradiation is one of techniques to improve genetic variability. The aims of this research were to obtain LD50, study the influence of irradiation, and genetic of mutation. To determine the LD50, seeds of soybean cv. Anjasmoro were irradiated with 0-800 Gy (100 interval) gamma rays. LD50 was calculated to be 202.5 Gy base on germination rate. Therefore, in further experiment 200-400 Gy (50 interval) dosage were used to irradiate seeds of soybean cv. Anjasmoro and Burangrang. All irradiation dosages (200-400 Gy) damage protoplast, decrease plant survival, prolong flowering dan harvest time, decrease plant height, number of total and filled pod, number of seeds, and improve the number of empty pod.

Perbanyakan dan Konservasi In Vitro Plasma Nutfah Talas (Colocasia esculenta (L.) Schoot) Nurwita Dewi; Bambang S. Purwoko; Ida Hanarida; Agus Purwito; Iswari S. Dewi
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p105-112

Taro is a potential source of carbohydratefor anticipation of climate change. In vitro technology havenot been widely implemented for tuber crops conservation.Conservation of the crops is mostly conducted in field. Suchconservation is very susceptible to biotic and abiotic stress.The research consisted of two activities i.e: micropropagationand conservation. The objectives were to obtain taro invitro propagation and conservation method. The trial wasarranged in a factorial design with six replications. Five taroaccessions were used as the first factor for each study. Thesecond factor in propagation study was propagation mediumi.e: MS; MS + 2.9 μM IAA + 4.4 μM BA and MS + 2.9 μM IAA+ 22,2 μM BA. Shoot tip from taro sucker was used asexplant. The second factor in conservation study was MSmedium containing mannitol (0, 30, 40, and 50 g/l). Twoleavesin vitro shoots from micropropagation study was usedas explants. The addition of BA in MS medium + 2.9 μM IAAincreased the number of shoot of taro germplasm. The bestmedium for micropropagation of taro germplasm No. 21 andTalas Jahe is MS + 2,9 μM IAA + 4,4 μM BA, whereas thebest medium for No. 503, Talas Jahe and Lumbu Banten isMS + 2,9 μM IAA + 22,2 μM BA. Based on data of plantheight, percentage of leaf life and shelf life, MS medium +manitol 40 g/l was the best medium for taro germplasmconservation with prolong sub-culture interval.

Regenerasi Jeruk Siam melalui Embriogenesis Somatik Ali Husni; Agus Purwito; Ika Mariska; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n2.2010.p75-83

The Regenerated of Siam Tangerin through SomaticEmbryogenesis. Ali Husni, Agus Purwito, Ika Mariska,and Sudarsono. Somatic embryogenesis occurs in mostplants that are cultured on a suitable medium in vitro.Somatic embryo may arise from single cells and the embryogeniccells are widely applicable in plant propagation,genetic manipulation and transgenic technologies. Thepresent study was carried out to develop an effectivesomatic embryogenesis technique to regenerate Siamtangerine plants. Materials used in this study were nucellartissues of young fruits (30-90 days post anthesis). Inductionof embryogenic calli was done by culturing the tissues onthree different basal media (MS, MW and MT). Embryomaturation was done on the MW medium + ABA (0; 0.1; 0.3;dan 0.5 mg/l), while germination to plantlet developmentwas done on WS medium + GA3 (0, 0.1, 0.3, and 0.5 mg/l).The results showed that among the three media, MW wasthe best medium for callus induction from nucellar tissueswith a success rates 98% for Simadu and 100% forPontianak. The best maturation of embryo somatic wasdone on MW medium + ABA 0.5 mg/l with success rates99%, while the best medium for germination and developmentinto planlets was MW medium + 0.5 mg/l GA3 with asuccess rate 58%.

Analisis Integrasi dan Segregasi Gen Ketahanan terhadap Hawar Daun pada Progeni F1 Hasil Persilangan Tanaman Kentang Transgenik dengan Non Transgenik Alberta Dinar Ambarwati; Agus Purwito; Muhamad Herman; S. M. Sumaraow; Hajrial Aswidinnoor
Jurnal AgroBiogen Vol 5, No 1 (2009): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n1.2009.p25-31

Potato late blight, caused by Phytophthora infestans is one of the most devastating plant diseases. Potato yield losses due to this disease ranged from 47-100%. Frequent intervals and high rates of fungicide spray, currently practiced by potato growers to control the disease are expensive. Host resistance is an alternative control measure that is more economically and environmentally sustainable. Development of late blight resistant plants was conducted by crossing RB transgenic Katahdin SP904 andSP951 as male and two susceptible (Atlantic, Granola) varieties as female parents. F1 progenies were molecularly characterized for the integration of the RB transgene and evaluated for their segregations. Crossing data of Atlantic x transgenic Katahdin SP904 and SP951 produced 71 (57.72%)berries with average number of seeds per berry 139.58 and 83 (41.29%) berries with average number of seeds/berry 85.23, respectively. Granola x transgenic Katahdin SP904 and SP951 crosses gave higher results in terms of berry set (79.55 and 84.44%, respectively) than Atlantic x transgenic Katahdin crosses. A total of 554 F1 progenies were analyzed for thepresence of the RB PCR marker. An expected 619-bp and 840-bp band were amplified in the progenies that contain the RB gene. The RB gene was integrated in 65 (45.45%), 77 (47.83%), 47 (45.63%), and 71 (48.30%) F1 progenies of Atlantic x transgenic Katahdin SP904, Atlantic x transgenic Katahdin SP951, Granola x transgenic Katahdin SP904, andGranola x transgenic Katahdin SP951, respectively. Chisquare tests showed that all the four cross combinations followed a 1 : 1 segregation ratio.

Induksi Kalus Embriogenik dan Daya Regenerasi Kopi Arabika Menggunakan 2,4- Dichlorophenoxyacetic Acid dan 6-Benzyladenine Meynarti Sari Dewi Ibrahim; Rr Sri Hartati; Rubiyo Rubiyo; Agus Purwito; Sudarsono Sudarsono
Jurnal Tanaman Industri dan Penyegar Vol 4, No 2 (2013): Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v4n2.2013.p91-98

Embriogenesis somatik kopi Arabika (Coffea arabica L.) masih mengalami kendala dalam meregenerasikan planlet dari eksplan yang dikulturkan. Kemampuan eksplan daun membentuk embrio dalam proses embriogenesis somatik kopi sangat dipengaruhi oleh komposisi media dan zat pengatur tumbuh. Penelitian bertujuan untuk melihat pengaruh pemberian 2,4-Dichlorophenoxyacetic Acid dan 6-Benzyladenine dalam proses pembentukan kalus embriogenik dan daya regenerasi kopi Arabika. Penelitian dilakukan di Unit Pengembangan Benih Unggul Pertanian, Badan Penelitian dan Pengembangan Pertanian pada bulan Juli 2011 sampai Desember 2012. Bahan tanaman yang digunakan adalah daun kopi Arabika varietas S795 koleksi Balai Penelitian Tanaman Industri dan Penyegar. Rancangan perlakuan menggunakan rancangan acak lengkap dengan 6 ulangan, masing-masing ulangan terdiri dari 5 eksplan. Induksi kalus menggunakan 5 kombinasi perlakuan 2,4-D 1 mg/l + BA 0 mg/l; 2,4-D 1 mg/l + BA 1 mg/l; 2,4-D 1 mg/l + BA 2 mg/l; 2,4-D 2 mg/l + BA 1 mg/l; dan kontrol (tanpa penambahan 2,4-D dan BA). Peubah yang diamati meliputi jumlah kalus, persentasi kalus embriogenik, berat basah kalus, dan jumlah proembrio. Hasil penelitian menunjukkan perlakuan 2,4-D 1 mg/l + BA 0 mg/l; 2,4-D 1 mg/l + BA 1 mg/l; 2,4-D 1 mg/l + BA 2 mg/l; dan 2,4-D 2 mg/l + BA 1 mg/l dapat membentuk kalus kecuali perlakuan kontrol. Berat kalus, persentasi kalus embriogenik, dan jumlah proembrio tertinggi diperoleh pada media kombinasi 2,4-D 2 mg/l dan BA 1 mg/l. Kalus yang mampu beregenerasi berasal dari media kombinasi 2,4-D 1 mg/l dan BA 2 mg/l dengan persentasi 16,67% dengan 6 kecambah per 0,2 gram kalus.Kata Kunci: Coffea arabica, 2,4-Dichlorophenoxyacetic Acid, 6-Benzyladenine, embriogenesis somatikRegeneration of planlets from cultured explants has been an obstacle in somatic embryogenesis of arabica coffee (Coffea arabica L.). The ability of leaf explants to generate embryos in somatic embryogenesis process of coffee was affected by composition of media and plant growth regulators. The objectives of the research was to examine the effect of 2,4-Dichlorophenoxyacetic Acid dan 6-Benzyladenine in the process of embryogenic callus and regeneration potential of arabica coffee. The study was conducted at Agricultural Superior Seed Development Unit, Indonesian Agency for Agricultural Research and Development (IAARD) from July 2011 to December 2012. Plant material used was leaves of S795 variety which is collected by Indonesian Industrial and Beverage Crops Research Institute. The research was arranged in completely randomized design with 6 replications, each replication consist of 5 explants. Callus induction used 5 treatments, i.e. 2,4-D 1 mg/l + BA 0 mg/l; 2,4-D 1 mg/l + BA 1 mg/l; 2,4-D 1 mg/l + BA 2 mg/l; 2,4-D 2 mg/l + BA 1 mg/l; and control (without 2,4-D and BA). Variables observed were number of callus, percentage of embryogenic callus, callus fresh weight and number of proembryo. Result showed that all treatments can produce the callus except control. Combination of 2,4-D 2 mg/l and BA 1 mg/l gave the highest of fresh weight of callus, percentage of embryogenic callus, and number of proembryo. Regenerating callus of 16.67% with the number of sprouts of 6 per 0.2 gram only derived from combination of 2,4-D 1 mg/l BA and 2 mg/l.

Co-Authors , Asnawatr , Nurhasanah , Rustikawati , Samanhudi , Suharsono , Supenti . LUKMAN A. Dinar Ambarwati Abimantara, Grandisa Cahya Agus Joko Santoso Agus Joko Santoso Alberta Dinar Ambarwati Alberta Dinar Ambarwati Alberta Dinar Ambarwati Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Ali Husni Alina Akhidaya Ambarwati, Alberta Dinar Ambarwati, Alberta Dinar Amrullah, Rizki Abi Anas D Susila Andri Ernawati Anjani, Imas Galuh Antonius Suwanto Arif Satria Arifin Noor Sugiharto Arifin Noor Sugiharto ARSYAD, MIRZA ARSIATY ARSYAD, MIRZA ARSIATY Asep Setiawan Atra Romeida Awang Maharijaya Bambang S. Purwoko Bambang Sapta Purwoko Branco, Luis Manuel Budi Marwoto Budi Marwoto Budi Winarto Catur Herison Chaireni Martasari Dewi Sukma Dian Puji Rahayu Didy Soepandi Didy Soepandi, Didy Didy Sopandie DINARTI, DINY Dini Dinarti Diny Dinarti DWI ANDREAS SANTOSA Dyah Manohara Dyah Retno Wulandari Dyah Retno Wulandari E. Suryaningsih Endah Retno Palupi Endang Gunawan ENDANG SUHENDANG Enny Sudarmonowati Enny Sudarmonowati Enny Sudarmonowati Eri Sofiari Erni Suminar Fathur Rahmi, Atika Fitri Rachmawati Fitri Yulianti G. A. Wattimena G. A. Wattimenal GA Wattimena GA Wattimena Gustaaf Adolf Wattimena Gustaff Adolf Wattimena Gustav Adolf Wattimena H . M. Machmud HAJRIAL ASWIDINNOOR Hartati, Raden Roro Sri Harti, Heri Herison, Catur Herman, Muhamad Herman, Muhammad I Made Arisudana Putra Ida Hanarida Ika Mariska Ika Mariska Imas Sukaesih Sitanggang Indriati Husain Inez H.S. Loeddin Suharsono Irdika Mansur ISKANDAR ZULKARNAEN SIREGAR ISMAIL MASKROMO Ismail Maskromo Iswari S. Dewi J. K. J. Laisina J. M. Pasaribu Juang Gema Kartika Julius D. Nugroho Kalsum Yulifar, Andi Sri Ummi Kartiman, Roni Kartiman, Roni Karyanti ,, Karyanti Kikin H Mutaqin Komang Trisna Wirakusuma Kosasih, Akhmad KRISTIANTO NUGROHO, KRISTIANTO Laela Sari Laela Sari Laela Sari laela Sari, laela LAREKENG, SITI HALIMAH M. Herman M. Machmud Maharijaya, dan Awang Marlin MASKROMO, ISMAIL Mastur Mastur Matjik, Nurhayati Anshori MATTJIK, NURHAYATI ANSHORI Megayani Sri Rahayu Memen Surahman Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Meynarti Sari Dewi Ibrahim Mia Kosmiatin Mia Kosmiatin Mia Kosmiatin Mira Humaira Mohamad Prayogi Muhamad Herman Muhammad Alwi MUHAMMAD HERMAN Muhammad Herman Muhammad Mahmud Muhammad Reza Zakie MUHAMMAD SYUKUR Ni Made Armini Wiendi Noor Farid Nur Laela Wahyuni Meilawati Nurhajati Ansori Mattjik NURHAYATI ANSHORI MATTJIK Nurhayati Ansori Mattjik NURITA TORUAN-MATHIUS Nurliani Bermawie Nurul Khumaida Nurwita Dewi Prasetyawati , Adinda Prima Muklisa PUJI LESTARI Purba, Dumaris Priskila Putra, Heriansyah Putri, Halida Adistya RAGAPADMI PURNAMANINGSIH Ragapadmi Purnamaningsih Ratna Trisnawati Ravenska, Nidya Rd. Selvy Handayani Rd. Zainal Frihadian REFLINUR REFLINUR Retno Prihatini Reza Ramdan Rivai Reza Ramdan Rivai Ridho Kurniati Ridho Kurniati Rizki Abi Amrullah Roedhy Poerwanto Roni Kartiman Roni Kartiman Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati RR. Sri Hartati Rr. Sri Hartati Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo Rubiyo S. M. Sumaraow S. M. Sumaraw S. Sudarsono Sari, Dewi Citra Sari, Laela SATRIYAS ILYAS Sembiring, Rinawati SIENTJE MANDANG SUMARAW Sientje Mandang Sumaraw SITI HALIMAH LAREKENG, SITI HALIMAH Slamet Susanto Slamet, Alim Setiawan Sobir Sobir Sri Rianawati Sudarmonowati, Enny Sudarsono Sudarsono SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono, dan SUDARSONO, nFn SUDARSONO, nFn SUDARSONO, SUDARSONO Sudirman Yahya Suhartanto , M. Rahmad Suhartanto, Muhammad Rahmad Sukma, Dewi Sumaraow, S. M. Sumaraw, S M Surjono Hadi Sutjahjo Suryanah Suryanah Suryaningsih, E. Suryo Wiyono Susiyanti . Suskandari Kartikaningrum Syaiful Anwar Syarifah Iis Aisyah TRI JOKO SANTOSO Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Tri Wiji Nurani Warid Warid Willy Bayuardi Suwarno Winarto, dan Budi Wulandari, Dyah Retno Wulandari, Dyah Retno Yopi Kurniawan

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