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All Journal MEDIA MEDIKA INDONESIANA International Journal of Science and Engineering (IJSE) Biosfer: Jurnal Tadris Biologi Jurnal Kesehatan Andalas Jurnal Berita Ilmu Keperawatan Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Dentino: Jurnal Kedokteran Gigi Jurnal Keperawatan Padjadjaran Inovasi : Jurnal Ekonomi, Keuangan, dan Manajemen Journal Of Holistic Nursing Science Pendas : Jurnah Ilmiah Pendidikan Dasar Jurnal Kreativitas PKM Jurnal Ilmu Kedokteran dan Kesehatan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Literasi : Jurnal Ilmu Pendidikan Interdental Jurnal Kedokteran Gigi (IJKG) Kumawula: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmu Kesehatan Jurnal Kebidanan Mutiara Mahakam PLAKAT : Jurnal Pelayanan Kepada Masyarakat Jurnal Kesehatan Pasak Bumi Kalimantan MAHESA : Malahayati Health Student Journal Jurnal Pengabdian Kepada Masyarakat Mulawarman Jurnal Medika: Karya Ilmiah Kesehatan Prosiding University Research Colloquium Jurnal Pengabdian Kepada Masyarakat: Kesehatan (JPKMK) Journal of Indonesia Vascular Access WASATHON TRIAGE MOTORIK INVOLUSI Mulawarman Dental Journal Prosiding Seminar Nasional Unimus (Medical Scientific Journal) MESINA Journal of Tropical Pharmacy and Chemistry Jurnal Sains dan Kesehatan Medical Journal of Soeradji
Endang Sawitri
Fakultas Kedokteran Universitas Mulawarman
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Journal : MEDIA MEDIKA INDONESIANA

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Pengaruh Pemberian Ekstrak Phyllanthus niruri Linn Terhadap Infiltrasi Limfosit dan Ekspresi Perforin pada Kanker Kolon Tikus Sprague-Dawley Endang Sawitri; Ign. Riwanto; Tjahjono Tjahjono; Edi Dharmana
MEDIA MEDIKA INDONESIANA 2013:MMI VOLUME 47 ISSUE 1 YEAR 2013
Publisher : MEDIA MEDIKA INDONESIANA

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ABSTRACTThe effect of Phyllanthus niruri Linn extract on lymphocytes infiltration and perforin expression in colon cancer of Sprague-Dawley RatBackground: Colon cancer treatment currently involves immunotherapy that aims to improve the quality of life and survival of patients. Phyllanthus niruri Linn (P. niruri L) may act as an immunomodulator, but its potency in antitumor immune responses has not been revealed. The study was conducted to evaluate the differences between the immunological status of rats suffering colon cancer which were not given to those given the extract of P. niruri L.Methods: The study was randomized posttest-only control group design. Samples were 30 Sprague-Dawley male rats, bodyweight 170-220 gr which induced by 1.2 DMH 30 mg/kgBW subcutaneously. On the weeks 9, 11 and 13, four induced rats each week were sacrified to detect the development of colon cancer. On the weeks of 13th all of 4 rats were developed colon cancer, so the induction were stopped. The rest of 18 induced rats were randomly into two groups: without P.  niruri L or positive control (K+)=9 rats and  given P. niruri L extract 13.5 mg/kg orally or  X  group=9  rats.  After 19th week all of rats were then terminated and tumor lesion of colon were processed hystophatologically. The tissues of colon cancer were stained by H&E for evaluate the lymphocytes infiltration and immunohistochemistry monoclonal antibody anti-perforin for perforin expression. Non pairs t-test was used with considered significant if p<0.05.Results: The mean of lymphocytes infiltration of the group X was 401.89±70.19, it was higher compared to K+ 191.89±50.68 (p=0.000). The mean percentage of perforin expression of group X was 39.00±1.80%, it was higher compared to K+ 23.00± 3.00% (p=0.000). Conclusion: The extract of P. niruri L increases immunologic status through mechanism of lymphocytes infiltration and perforin expression elevation of colon cancer in animal mode.Keywords : Phyllanthus niruri Linn, colon cancer, lymphocytes infiltration, perforinABSTRAKLatar belakang: Penanganan kanker kolon saat ini melibatkan imunoterapi yang bertujuan untuk meningkatkan kualitas hidup dan survival penderitanya. Phyllanthus niruri Linn bekerja sebagai imunomodulator, tetapi potensinya dalam respons imun antitumor belum banyak diungkap. Penelitian bertujuan untuk mengevaluasi perbedaan status imunologis antara tikus coba yang menderita kanker kolon yang tidak diberi dengan yang diberi ekstrak P. niruri L.Metode: Penelitian ini merupakan randomized posttest-only control group design. Sampel berupa 30 ekor tikus Sprague-Dawley jantan, yang diinduksi 1,2 DMH 30 mg/kgBB subkutan sekali setiap minggu. Pada minggu ke-9, 11 dan 13 masing-masing empat ekor tikus dimatikan untuk melihat perkembangan tumor. Minggu ke-13 pada kolon empat tikus telah tumbuh kanker kolon, sehingga induksi dihentikan dan sisa 18 tikus dirandom alokasi menjadi 2 kelompok. Kelompok kontrol positif (K+) tanpa pemberian P. niruri L (9 tikus) dan kelompok diberi P. niruri L. 13,5 mg/kg per hari melalui sonde (9 tikus). Minggu ke-19 semua tikus diterminasi, lesi tumor pada kolon diproses menjadi sediaan histopatologik, kemudian dipulas dengan H&E untuk memeriksa infiltrasi limfosit dan pulasan imunohistokimia menggunakan antibodi monoklonal anti-perforin untuk melihat ekspresi perforin. Analisis data menggunakan uji t tidak berpasangan dengan derajat signifikansi p<0,05.Hasil: Rerata jumlah infiltrasi limfosit kelompok X adalah 401,89±70,19 lebih tinggi dibanding kelompok K+ yaitu191,89±50,68 (p=0,000). Persentase rerata ekspresi perforin kelompok X sebesar 39,00±1,80%, lebih tinggi dibandingkan dengan K+ yakni 23,00±3,00% (p=0,000).Simpulan: Ekstrak P. niruri L meningkatkan status imunologi melalui mekanisme peningkatan infiltrasi limfosit dan ekspresi perforin untuk melawan kanker kolon pada tikus coba
Ekstrak Phyllanthus niruri Linn, Pertumbuhan Tumor dan Proliferasi Sel Kanker Kolorektal: Studi Eksperimental pada Tikus Sprague-Dawley yang Diinduksi 1,2 DMH Endang Sawitri; Ign. Riwanto; Tjahjono Tjahjono; Edi Dharmana
MEDIA MEDIKA INDONESIANA 2012:MMI VOLUME 46 ISSUE 1 YEAR 2012
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

Effect of Phyllanthus niruri Linn extract on tumor growth and cell proliferation of colorectal cancer: experimental study in 1.2 DMH-induced Sprague-Dawley ratsBackground: The development of chemotherapy for colorectal cancer (CRC) is so very advanced, however, the survival of patients has not been satisfactory, therefore, the current therapy also involve immunotherapy. Phyllanthus niruri Linn (P. niruri L) may act as an immunomodulator and anticancer, but its potency has not been revealed. Study was conducted to confirm the effects of P. niruri L extract in the treatment of CRC.Method: The study was randomized posttest-only control group design. Samples were Sprague-Dawley male rats, bodyweight 170- 220 gr, were divided into two groups: non induced or negative control (K-) consisted of 9 normal rats, and induced by 1.2 DMH 30 mg/kgBW subcutaneously group consisted 30 rats. On the weeks 9, 11 and 13, four induced rats each week were sacrified to detect the development of CRC. On the weeks of 13th all of 4 rats were developed CRC, so the induction were stopped. The rest of 18 induced rats were randomly into two groups: without P. niruri L or positive control (K+)=9 rats and given P. niruri L extract 13.5 mg/kg orally or X group=9 rats. After 19th week all of rats were then terminated and tumor lesion of colon were examined macroscopically and hystophatologic tissues were stained with AgNORs for evaluate the cells proliferation. Oneway Anova and Post Hoc LSD test for the growth of colon tumor and non pairs t-test for cell proliferation were used. Considered significant if p was <0.05. Result: There was no tumor growth on K- group, while for K+ was 83.33±14.34% and on X was 40.44±13.23% (p=0.000). The mean of AgNORs on K+ was 4.60±0.55 while on X was 2.25±0.39 (p=0.000).Conclusion: The extract of P. niruri L suppress the tumor growth and cell proliferation of CRC.Keywords: Phyllanthus niruri Linn, colorectal cancer, tumor growth, proliferationABSTRAKLatar belakang: Perkembangan kemoterapi untuk kanker kolorektal (KKR) sangat maju, tetapi kelangsungan hidup penderitanya belum memuaskan, sehingga penanganan multimodalitas juga melibatkan imunoterapi yang bertujuan untuk meningkatkan kualitas hidup. Phyllanthus niruri Linn dapat bekerja sebagai imunomodulator sekaligus antikanker, tetapi potensinya belum banyak diungkap. Penelitian bertujuan untuk menganalisis pengaruh pemberian ekstrak P. niruri L terhadap pertumbuhan tumor danproliferasi sel KKR.Metode: Penelitian ini merupakan randomized posttest-only control group design. Sampel berupa tikus Sprague-Dawley jantan, dibagi 2: kelompok tanpa induksi 1,2 DMH dan tanpa P. niruri L (K-: kelompok kontrol negatif) sebanyak 9 ekor dan kelompok induksi 1,2 DMH 30 mg/kgBB subkutan setiap minggu sebanyak 30 ekor. Pada minggu ke-9, 11 dan 13 masing-masing empat ekor tikus dibunuh untuk melihat perkembangan tumor. Pada minggu ke-13 keempat tikus telah berkembang menjadi KKR, induksidihentikan dan sisa 18 tikus dirandom alokasi menjadi 2 kelompok. Kelompok kontrol positif (K+) tanpa pemberian P. niruri L (9 tikus) dan kelompok diberi P. niruri L. 13.5 mg/kg per hari melalui sonde (9 tikus). Minggu ke-19 semua tikus diterminasi, diperiksa lesi tumor makroskopik pada kolon. Jaringan histopatologik diwarnai AgNORs untuk memeriksa proliferasi sel. Analisis data meliputi analisis deskriptif dan uji Oneway Anova, dilanjutkan dengan post hoc test LSD untuk pertumbuhan tumor kolon dan uji ttidak berpasangan untuk proliferasi sel. Derajat signifikansi yang dipilih adalah p<0,05. Hasil: Tidak ditemukan pertumbuhan tumor pada kelompok K-. Persentase rerata pertumbuhan tumor kelompok X sebesar40,44±13,23%berbeda sangat signifikan (p=0,000) dibanding K+ sebesar 33±14,34%. Rerata bercak AgNORs kelompok K+sebesar 4,60±0,55 dan menurun pada kelompok X (2,25±0,39), terdapat perbedaan yang sangat signifikan pada kedua kelompok (p=0,000). Simpulan: Ekstrak P. niruri L menekan pertumbuhan tumor dan proliferasi sel kanker kolorektal.
Co-Authors - Setianingsih Achmad, Andika Adi Saputra Ade Tutti Rokhayati Rosa Agus Murtana Agus Sudaryanto Agustina Rahayu Magdaleni Agustiningrum, Ratna Ahmad Fikri Akbar Alvina Hafidiati Anggita Maharani Anik Puji Rahayu Anjani, Azzahra Primadivta Anna Uswatun Qoyyimah Annisa Muhyi Apriana Apriana Apriana, Yuyun Apriani T Arie Ibrahim Arius, Yudhy Aryani Dwi Kesumawardani Atika, Andi Rafika Auralia, Alifia Averina Dwie Larensia Azi Surya Kurniawan Bakhtiar, Rahmat Boenjamin, Abdul Azis Boyke Rorimpandey Boyke Soebhali, Boyke Chory Elsera Cicih Bhakti Purnamasari Damayanti, Anissa Danial - Daryani Devi Permata Sari Dewi Setyowati Dewi Suryandari Dewi, Januartha Kisari Diastuti, Denti Dynamurti Wintoro, Piscolia Edi Dharmana Edi Dharmana Elsera, Chori Endang Wahyuningsih Enden Sirojudin Enggarita Widyahapsari Erwin Ginting Esmaya R. A. Adaniyah Esri Rusminingsih - Evi Fitriany Falah, Irdiansyah Al Farida Nugrahani Fatimah Nur Lintang Fauzan, Muh . Febriati, Desi Fiani, Raicha Okta Fikriah, Ika Fitri Suciana Fitriana Noor Khayati Hadiyanti Hafidhuddin, M. Abdul Aziz Hafidhuddin, Muhammad Abdul Aziz Hajari, Siti Hamdi Mayulu Hanis Kusumawati Rahayu Hardianto, Djoen Hary Nugroho Hary Nugroho Hazami, Lathifah Nur Islami B. Ida Ayu Kade Sri Widiastuti Idris, Fagil Rananda Ign. Riwanto Ign. Riwanto Ilham Akbar Choirul Umam Indra Sukmana Putra Indri Septiana Irawiraman, Hadi Irsan Tricahyadinata Iwan Samsugito Khumaidi Khumaidi Kombong, Aldri Yithream Krispinus Duma Kusumaningrum, Puput Risti Kusumawati , Ema Lilies Anggarwati Astuti Listiyawati Magdaleni Agustina Rahayu Magdaleni, Agustina Rahayu Maghfiroh, Qonita Yaumil Maharani, Kinanti Nur Cantika Maina, Annin Dya Mangara Tua, Ivan Joalsen Martalina, Elliana Marwanti Masyudhi Masyudhi Maulida, Nabila Mawardi , Mawardi Moriko Pratiningrum Muhammad Aminudin Muhammad Fachrian Akbar Muhammad Furqan, Muhammad Muhammad Rezwan Qhuzairi Musnar Munir Muthi’ah, Nisa Mu’ti, Abdul Nabiela An’nisa Putri Nabilah, Nur Alya Nadhifa Reynanda Nanda, A. Luthfiah Novia Fransiska Ngo Nur Indranettasa, Anes Nur Khoma Fatmawati Nur Khoma Fatmawati Nur Wulan Agustina Nur ‘Azah Nurul Budiana Nurul Fadhila Lestari Nurul Hasanah Nurul Hasanah Nurul Hasanah Nuryanni Dihin Utami, Nuryanni Dihin Oktaviani, Uus Seila Poppy Desra S Nasution Pramasari, Cristiani Nadya Pramesti, Nadya Ashiilah Syifa Pramulia Yudia, Riries Choiru Pratiningrum, Moriko Prinadira, Sarah Puput Risti Kusumaningrum Purnamasari, Cicih Bhakti Putra, Indra Sukmana Putri, Nabiela Annisa Putri, Zeni Evilya Qhuzairi, Muhammad Rezwan Rachman, Syarifah Awwaliyyah Rahma, Rosyida Muthia Rahmat Bakhtiar Ramadhan, Muhammad Aidil Aulia Retnaningrum, Yuliana Rahmah Ridwan M Ridwan, Muhammad Thoyib Rimayanti, A. Annisa Aulia Riries Khoiru Rizky Setya Tama Rohmawati, Wiwin Romadhani Tri Purnomo Safitri, Nurvita Salsabila, Naila Cantika Salsabila, Salma Nadya Sanjaya, Regina Anastasia Saputra, Rudi Saputri, Ni Made Maharani Indira Sari, Aulia Novita Sari, Vivi Arsi Arsi Rahmania Sari, Vivi Arsi Rahmania Sembiring, Rinawati Setianingsih Setianingsih, S Silfia Purnawati Sinar Yani Sinar Yani Siti Khotimah Siti Khotimah Siti Nur Azizah Sjarif Ismail Sofia Apriyanti Sri Wahyuni Sri Wahyunie Subagio, Kiki Jayapratama Sudarso, Sulistiawati Sulistiani, Dewi Sulistiani, Dewi Arsih Sulistiawati Sudarso Sulistiawati Sulistiawati Sulistiawati Sulistiawati Sulistiyawati Sulistiyawati Supardi Supardi Supardi Suwarto Suwarto Swandari Paramita Syaiful Yanwar Syifa Fitria Anggraeny Taufan Purwokusumaning Daru, Taufan Purwokusumaning Tetrine Prameswari Tia Mutia Tjahjono Tjahjono Tjahjono Tjahjono Tua, Ivan Joalsen Mangara Tumenggu, Auliya Rizky Ulyah, Rochimah Thul Virginia Uspa, Astria Wafi, Viky Hibatu Wati, Cindy Ira Wati, Eka Rina Wijaya, Joseffine Valentina Wiranti, Patih Atma Wirayuda, M. Ade Wiryawan, I Nyoman Bagus Putra Wiwin Rohmawati Yani, Sinar Yasmiin Khoirul Bariyyah Yoga Bowo Leksono Yudia, Riries Choiru Pramulia Yuniati Yuniati ‪Arlina Dhian Sulistyowati‬