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Suharsono Suharsono
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Journal : JURNAL BIOLOGI INDONESIA

 1 
Efikasi Cendawan Entomopatogenik Untuk Mengendalikan Ulat Bulu Prayogo, Yusmani; Marwoto, Marwoto; Suharsono, Suharsono
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3068

Abstract

The efficacy of entomopthogenic fungus for Tussock Moth Caterpillars Control. The occurrence of Tussock Moth Caterpillar (TMC) out break reported in some areas in East Java at Probolinggo, Malang, Batu and other districts were worried. The high caterpillar population not only attacking mangos, cherry, and wild watercress, but also migrating to living house before pupation. The available control of these caterpillar is chemical pesticide. However, thechemical will kill the natural enemies and other beneficial insects. A laboratory experiment to study the efficacy of five entomopathogenic fungus against TMC and pupae was conducted at the Entomology Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) in April 2011. Our experiment was arranged using completely randomized design (CRD) with three replicates using five entomopthogenic fungus. The efficacy was determined by the mortality of caterpillar/larva and pupae infected by the fungus. Result showed that these five fungus had not effectively kill the caterpillar, as the dense setae in integument acted as a mechanical barrier to fungus infection. The pupal stage was more susceptible to fungus infection. It was found that Paecilomyces fumosoroseus and Lecanicillium lecanii fungus were more effective than Metarhizium anisopliae, Beauveria bassiana and Nomuraea rileyi repectively. These two fungus could kill 90% of pupae and also were easier to be cultured in a wide range of natural media. Therefore P. fumosoroseus and L. lecanii are potentially used as a biological control agent against TMC out break.Keywords: Entomopathogenic fungus, hairy caterpillar, incubation period, colonization
Pengaruh Radiasi Sinar Gamma dan Asam Fusarat untuk Meningkatkan Ketahanan Abaka (Musa textilis Nee) terhadap Fasariurn oxysporum Damayanti, Fitri; Suharsono, Suharsono; Mariska, Ika
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.082 KB) | DOI: 10.14203/jbi.v3i4.3285

Abstract

ABSTRACTEffect of gamma radiation and fusaric acid for resistance to wild Fusarium disease onabaca plant (Mum textillis Nee). The problem in abaca production is wilt disease infectioncaused by Fusarium oxysporzim. The resistant variety against the pathogen has not beenavailable yet. The disease resistance character of the species might be improved throughsomaclonal variation and in vitro selection. Different pure toxin of fusaric acid concentration(0, 15,30,45,60, and 75 mg/l) was used as component selection to get new hope numbers ofresistant abaca to wilt Fusarium disease through in vitro selection. Concentration of 45 mgllfusaric acid is lethal for abaca, so we used this concentration as dose of selection to selectshoots from irradiated calli. Gamma irradiation was used as mutagent to increase somaclonalvariation on abaca. Six levels of gamma-ray radiation (0, 0.5, 1, 2, and 3 Krad) were appliedto embriogenic calli. Increasing dose of radiation decreased the viability of calli. LDso wasfound between 1-1.5 Krad of radiation dose. In vitro selection was carried out in two stages.The concentration of selection of hsaric acid in stage I1 was increased one level to theconcentration in stage 1. Stage I selection of shoots from irradiated calli on mediumcontaining pure toxin 45 mg/l fusaric acid, showed that the survival capacity decreasing withthe increasing doses of gamma irradiation. In stage 11, shoots from irradiated calli (at 0.5 and1 Krad) could survive on medium containing 60 mg/l fusaric acid. In medium selectioncontaining 50% filtrate F. oxysporum, fusaric acid resistant shoots were also filtrate resistant.There was a correlation between in vitro fusaric acid and filtrate of F. oxysporum resistantplant and conidia suspension of F. oxysporum resistkt plant in the greenhouse.Keywords: Gamma radiation, in vitro selection, fusaric acid, Musa textilis, Fusariumoxysporum
EFIKASI CENDAWAN ENTOMOPATOGENIK UNTUK MENGENDALIKAN ULAT BULU Prayogo, Yusmani; Marwoto, Marwoto; Suharsono, Suharsono
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3068

Abstract

The efficacy of entomopthogenic fungus for Tussock Moth Caterpillars Control. The occurrence of Tussock Moth Caterpillar (TMC) out break reported in some areas in East Java at Probolinggo, Malang, Batu and other districts were worried. The high caterpillar population not only attacking mangos, cherry, and wild watercress, but also migrating to living house before pupation. The available control of these caterpillar is chemical pesticide. However, thechemical will kill the natural enemies and other beneficial insects. A laboratory experiment to study the efficacy of five entomopathogenic fungus against TMC and pupae was conducted at the Entomology Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) in April 2011. Our experiment was arranged using completely randomized design (CRD) with three replicates using five entomopthogenic fungus. The efficacy was determined by the mortality of caterpillar/larva and pupae infected by the fungus. Result showed that these five fungus had not effectively kill the caterpillar, as the dense setae in integument acted as a mechanical barrier to fungus infection. The pupal stage was more susceptible to fungus infection. It was found that Paecilomyces fumosoroseus and Lecanicillium lecanii fungus were more effective than Metarhizium anisopliae, Beauveria bassiana and Nomuraea rileyi repectively. These two fungus could kill 90% of pupae and also were easier to be cultured in a wide range of natural media. Therefore P. fumosoroseus and L. lecanii are potentially used as a biological control agent against TMC out break.Keywords: Entomopathogenic fungus, hairy caterpillar, incubation period, colonization
PENGARUH RADIASI SINAR GAMMA DAN ASAM FUSARAT UNTUK MENINGKATKAN KETAHANAN ABAKA (MUSA TEXTILIS NEE) TERHADAP FASARIURN OXYSPORUM Damayanti, Fitri; Suharsono, Suharsono; Mariska, Ika
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i4.3285

Abstract

ABSTRACTEffect of gamma radiation and fusaric acid for resistance to wild Fusarium disease onabaca plant (Mum textillis Nee). The problem in abaca production is wilt disease infectioncaused by Fusarium oxysporzim. The resistant variety against the pathogen has not beenavailable yet. The disease resistance character of the species might be improved throughsomaclonal variation and in vitro selection. Different pure toxin of fusaric acid concentration(0, 15,30,45,60, and 75 mg/l) was used as component selection to get new hope numbers ofresistant abaca to wilt Fusarium disease through in vitro selection. Concentration of 45 mgllfusaric acid is lethal for abaca, so we used this concentration as dose of selection to selectshoots from irradiated calli. Gamma irradiation was used as mutagent to increase somaclonalvariation on abaca. Six levels of gamma-ray radiation (0, 0.5, 1, 2, and 3 Krad) were appliedto embriogenic calli. Increasing dose of radiation decreased the viability of calli. LDso wasfound between 1-1.5 Krad of radiation dose. In vitro selection was carried out in two stages.The concentration of selection of hsaric acid in stage I1 was increased one level to theconcentration in stage 1. Stage I selection of shoots from irradiated calli on mediumcontaining pure toxin 45 mg/l fusaric acid, showed that the survival capacity decreasing withthe increasing doses of gamma irradiation. In stage 11, shoots from irradiated calli (at 0.5 and1 Krad) could survive on medium containing 60 mg/l fusaric acid. In medium selectioncontaining 50% filtrate F. oxysporum, fusaric acid resistant shoots were also filtrate resistant.There was a correlation between in vitro fusaric acid and filtrate of F. oxysporum resistantplant and conidia suspension of F. oxysporum resistkt plant in the greenhouse.Keywords: Gamma radiation, in vitro selection, fusaric acid, Musa textilis, Fusariumoxysporum
Co-Authors AA Sudharmawan, AA Adi Isworo, Adi Adikara, Tatang Santanu Agung Setiabudi Agung Setiabudi, Agung Aini, Kuratul Akhmad Solikhin AKIHO YOKOTA Al arif, Mohammad Anam Al Firah, Al Firah ALEX HARTANA Alimuddin, Khasan Ana Quthratun Nada Angga Sugiarto Anik Herminingsih Apon Zaenal Mustopa Arif Rianto Budi Nugroho Aris Tjahjoleksono Arwani Arwani Ashadi Ashar N, Novema Aspariansyah, Aspariansyah Asrul Muhamad Fuad, Asrul Muhamad Astanto Kasno Avellyn Shinthya Sari Aviadi, Dedi Badriah, Liah Baehaqi Bambang Sarwono Bambang Yulianto Budianingsih, Budianingsih Cahyadi, Tedy Agung Caswita Caswita Cici Indriani Dalimunthe damayanti, dini Dani Ramdani, Dani Dessy Adelin Destiana, Destiana Didy Sopandie Diki Muhamad Chaidir Diponegoro, Muhammad Djaka Marwasta Egi, Egi Nuryadin Eko Teguh Paripurno Elfa Cahya Jamiati Endang Surahman Erawati, Erna Fachry Abda El Rahman Faisal Rachman Febriana Dwi Wahyuni Feri, Zefki Okta Ferry Faisal, Ferry Firdaus, Syarifin Fitri Damayanti Fitri Damayanti Fitri Wibowo Fitri, Novi Aryani Fitriya, Nuriyatul Gandhi Sutjahjo Gesa Cantya Primatara Gunawan, Heru Hadiarto, Toto Hamim Hamim Hapsari, Agustina Retno Harijani, Nenny Hariyadi, Rahmat Hasan Labiqul Aqil Hermana, Salma Junaina Hidanah, Sri Hutabarat, Madya I, Heriberta Reny Idayati, Tutik Rosita Idha Susanti Iif Rahmat Fauzi, Iif Rahmat Ika Barokah Suryaningsih Ika Mariska IMAM MUSTOFA Irbah, Ariq Nurjannah Ishaq, Rizal Maulana Jayawarsa, A.A. Ketut Kagoya, Tundani Kartawan, Kartawan Karuwal, Ritha Lusian KINYA AKASHI Komul, Marsha Krisdiana Wijayanti Kristanto, Paustinus Edi Kusuma, Amino V.A. Kusumayudha, Sari Bahagiarti Kuswanto, Edi Lucia Endang Hartati, Lucia Endang Lulut Dwi Sulistyaningsih Lulut Handayani Maemonah, Maemonah Mahmudah, Uswatun marwoto marwoto Meta, Raharni Rosfiyanti Miftahudin . Mochamad Widjanarko Muhammad Helmi Muhammad Jusuf Muhammad Sirril Wafa Muin, Muhammad Rusli Munasik Munasik Mustafawi, Wike Z. Mustofa, Romy Faisal Mustopa, Apon Z. Mutiara, Frista Mycelia Paradise Nabiila, Alyaa Nainggolan, Adinda Rachel Nasrul, Herni Widiyah Ningrum, Hanis Rachna Noka, Isara Abda Noor Faidah, Noor Noorikhsan, Faisal Fadilla Novriyandi Hanif Nugroho, Arif Rianto Budi Nugroho, Arif Riyanto Budi Nur Izzati, Aisyah Nuriyatul Fitriya Nurjanah, Fitri Nuryadin, Egi Oktavianti Oktavianti Palaniappan, Ananda Kumar Pausta Yugianus Pertiwi, Nadira Rasya Polosoro, Aqwin Pramono, Sidik Prasetya, Wandy Murti Prasetyadi, C Prasetyadi, Carolus Prasetyadi, Corolus Pratama, Yudha Agung Pujiastuti, Raden Rara Sri Endang Pujiastuti, Rr Sri Endang Pujiastuti, Rr. Sri Endang purwoko, devit Raden Roro, Brilianti Chrisnajayantie RADITE TISTAMA Rasyidina, Sumayya Ainur RATIH DEWI HASTUTI Rikoh Manogar Siringoringo Rinaldi Rizal Putra, Rinaldi Rizal Rivai, Muhamad Rizal Rochadi Abdulhadi Rohananingsih, Rohananingsih Rojabtiyah, Rojabtiyah Rondhianto Rondhianto S. Hidayat, S. Safari, Neng Ayu Mustika Sahputra, Ngatno Sahroni, Sahroni Sahupala, Sapiah Samuel Agus Triyanto, Samuel Agus Santika, Wulan Puja Saputra, Muhammad Wahyu Saputri, Niken Anggraini Sri Sari Bahagiarti Kusumayudha Sari Bahagiarti Kusumayudha Sari, Avellyn Shintya Sari, Fatihah Fajar Sarmanu, Sarmanu Sarsidi Sastrosumarjo Selfianie, Maulidia Septiana, Erni Setiahadiwibowo, Ajimas Pascaning Shohifaha , Salsabila Sholihin, Achmad Simanjuntak, Veronica Sirait, Budi Chrismanto Sirojuddin, Wildan Siti Zulaeha, Siti sodiq, alwi mahardhika Soekarta, Rendra Solly Aryza Sri Hidanah Sri Langgeng Ratnasari SRI RAHAYU Sri Sumarni Sugiyarto, Angga Sulistiani, Erina Sulistyaningsih, Yohana C Sulistyowati, Dina Indrati Dyah Sunarko Sunarko Supena, Ence Darmo Jaya Supranianondo, Koesnoto Susanto, Abiyyu Naufal Sutarto Sutarto Sutrisno, Sutrisno Suyanta Suyanta Ta'adi, Ta'adi Teuku Tajuddin Tita Damayanti Lestari Titik Sudartinah Titin Handayani Trisnaningrum, Niken Trisnowati, Yuni Dwi Trustinah Trustinah Ulfah Juniarti Siregar Utut Widy Astuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Vita Meylani Wahyu Hidayat Wamiliana Wamiliana Wendry Setiyadi Putranto Wening Enggarini Widya Paramita Lokapirnasari Widyastuti, Arum WIJAYANTO WIJAYANTO Winarso, Widyo Wiwik Misaco Yuniarti YanRiska Venata Sembiring, YanRiska Venata Yeni Yulistanti Yohana Noradika Maharani Yulianto, Bernawan Yuniati, Ratna YUSMANI PRAYOGO Zainal Muttaqin zulfitra, Zulfitra