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All Journal Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Lingkungan GRADASI Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat BERITA BIOLOGI Panrita Abdi - Jurnal Pengabdian pada Masyarakat Omni-Akuatika Jurnal Matematika Sains dan Teknologi EnviroScienteae Jurnal Akuakultur Sungai dan Danau Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Mina Sains Jurnal Kelautan Nasional Jurnal Kebijakan Sosial Ekonomi Kelautan dan Perikanan Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Demandia : Jurnal Desain Komunikasi Visual, Manajemen Desain, dan Periklanan JURNAL PERIKANAN TROPIS Jurnal Abdi Insani PELAGICUS: Jurnal IPTEK Terapan Perikanan dan Kelautan JAGO TOLIS : Jurnal Agrokompleks Tolis Buletin Jalanidhitah Sarva Jivitam Clarias : Jurnal Perikanan Air Tawar Jurnal Pusat Inovasi Masyarakat Jurnal Salamata Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan JARI : Jurnal Akuakultur Rawa Indonesia

Eddy Supriyono

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, IPB University, Jalan Raya Dramaga Kampus IPB, 16680, Jawa Barat, Indonesia

Author-ID : 476534

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Performance evaluation of micro bubble generator on physiological response of Nile tilapia Oreochromis niloticus (Linnaeus, 1758) farmed at different densities in recirculating aquaculture system Sri Wahyuni Firman; Kukuh Nirmala; Eddy Supriyono; Nurul Taufiqu Taufiqu Rochman
Jurnal Iktiologi Indonesia Vol 19 No 3 (2019): October 2019
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v19i3.504

Micro-bubble generator is a device to maintain water quality by producing micron-sized bubbles. This study aims to evaluate the application of micro-bubble generator in different density on production performance and physiological response of nile tilapia reared in recirculating system. An experiment was designated by performing 3 densities, namely 15 , 30, and 45 individuals 60 L-1 with 3 replications. Every single unit of experiment applies a 34×42×41 cm3 maintenance container. The length size of fish used in this study was 7.44±2.89 cm and body weight of 10,96 ± 0,53 g. Fish were reared for 42 days in recirculation water system and fed ad satiation. The results of the evaluation of the physiological response showed that fish kept at density up to 45 60 L-1 did not show any changes, indicating that fish was in stress condition. The best production performance was in treatment A (15 individuals 60 L-1) with a specific growth rate of 1.87 ± 0.15% and a feed conversion ratio of 0.95 ± 0.08. Abstrak Pembangkit gelembung mikro merupakan suatu alat yang dapat menghasilkan gelembung udara berukuran mikro. Penelitian ini bertujuan untuk mengevaluasi penggunaan pembangkit gelembung mikro pada kepadatan yang berbeda terhadap performa produksi dan respons fisiologis ikan nila yang dipelihara dalam sistem resirkulasi. Penelitian ini dilakukan dengan metode eksperimental dengan tiga perlakuan kepadatan yaitu 15 ekor, 30 ekor dan 45 ekor 60 L-1 yang diulang sebanyak tiga kali. Wadah pemeliharaan berukuran 34×42×41 cm3. Ukuran ikan yang digunakan dalam penelitian ini memiliki panjang 7,44±2,89 cm, serta bobot 10,96±0,53 g. Aplikasi pembangkit gelembung mikro dibe-rikan pada awal hingga akhir pemeliharaan selama 42 hari dengan sistem resirkulasi dan ikan diberi pakan secara ad satiation. Hasil evaluasi terhadap respons fisiologis menunjukkan bahwa ikan yang dipelihara hingga kepadatan 45 ekor 60 L-1 tidak menunjukkan adanya perubahan yang mengindikasikan ikan mengalami stress. Performa produksi terbaik adalah pada perlakuan A (15 ekor 60 L-1) dengan laju pertumbuhan spesifik 1,87±0,15% dan nisbah konversi pakan 0,95±0,08.

Production performance and physiology response of Anguilla bicolor bicolor rearing with a wet, damp and dry system Eko Harianto; Eddy Supriyono; Tatag Budiardi; Ridwan Affandi; Yani Hadiroseyani
Jurnal Iktiologi Indonesia Vol 20 No 2 (2020): June 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i2.519

Aquaculture is is often constrained by the availability of water, both in quality and quantity. The aim of this study was to determine the optimum maintenance system of elver (Anguilla bicolor bicolor) on a wet, moist and dry system through production performance studies and physiological responses. The research was conducted from August to September 2018 at the Production Technic and Management of Aquaculture Laboratory, Department of Aquaculture, IPB University. A completely randomized design with 5 treatments and 3 replications was used in this study, namely treatment with high water wet system 0% of body height (A), high water wet system 50% of body height (B), high water wet system 100% of body height (C), moist system (D), and dry system (E). The body weight of elver was 18.80 ± 0.62 g which derived from fish cultivator in Bogor, West Java. Fish was fed 3 times per day at satiation with commercial feed which a protein content of 50%. Results showed that treatment C was the best result with survival rate of 100%, the specific growth rate of 0.45% day -1, the absolute growth rate of 0.07 g day 1 and feed conversion ratio of 6.57. The analysis of variance showed that the treatments showed the significant effect (P <0.05). The blood glucose value closest to the eel condition before treatment was found in the treatment C. Abstrak Budi daya ikan sering terkendala terhadap ketersediaan air, baik kualitas maupun kuantitasnya. Ikan sidat (Anguilla bicolor bicolor) merupakan spesies ikan yang dalam sistem pemeliharaannya membutuhkan volume air yang banyak. Tujuan penelitian ini adalah menentukan sistem pemeliharaan terbaik elver ikan sidat (A. bicolor bicolor) pada sistem basah, lembap, dan kering melalui kajian kinerja produksi dan respons fisiologis. Penelitian dilakukan dari bulan Agustus sampai September 2018 di Laboratorium Teknik Produksi dan Manajemen Akuakultur, Departemen Budidaya Perairan, Institut Pertanian Bogor. Penelitian menggunakan rancangan acak lengkap 5 perlakuan dan 3 ulangan, yaitu perlakuan sistem basah tinggi air 0% dari tinggi badan (A), sistem basah tinggi air 50% dari tinggi badan (B), sistem basah tinggi air 100% dari tinggi badan (C), sistem lembap (D), dan sistem kering (E). Ikan yang digunakan adalah elver ikan sidat berukuran 18,80±0,62g yang berasal dari pembudidaya di Bogor Jawa Barat. Pakan yang digunakan adalah pakan komersial dengan kadar protein 50% yang diberikan secara at satiation 3 kali per hari. Hasil penelitian menunjukkan bahwa perlakuan C memberikan hasil terbaik dengan nilai sintasan 100%, laju per-tumbuhan spesifik 0,45 % hari-1, laju pertumbuhan mutlak 0,07 g hari-1 dan rasio konversi pakan sebesar 6,57. Hasil analisis ragam menunjukkan bahwa perlakuan memberikan pengaruh yang nyata (p<0,05). Nilai glukosa darah pada perlakuan C merupakan nilai yang paling mendekati dengan kondisi ikan sidat sebelum perlakuan.

The color quality of Sumatra barb Puntigrus tetrazona (Bleeker, 1855) in different light spectrum exposure Wijianto Wijianto; Kukuh Nirmala; Yuni Puji Hastuti; Eddy Supriyono
Jurnal Iktiologi Indonesia Vol 20 No 3 (2020): October 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i3.534

The color quality of Sumatra barb that cultivated by the farmers are not as good as the fish that collected from the wild. One of the causes is the unsuitable environment for maintaining and breeding Sumatran barb. This research aims to compare color quality of Sumatra barb Puntigrus tetrazona by exposure the different light spectrums on maintenance media. The experiment was completely randomize design with six treatments and three replications consisted of K (control), R (room light), M (red light spectrum), H (green light spectrum), B (blue light spectrum) and P (white light spectrum). The method used to measure Sumatra barb color quality using Photoshop CS 5 software and chromatophore cell calculations. The results of color quality analysis after 28 showed that the (M) treatment had the highest percentage of color quality was 48.81 ± 1.57% for orange color and 32.26 ± 0.07% for black color. The highest number of chromatophore cells was in M treatment with 147 ± 3.7 cells mm-². The red light spectrum (M) treatment showed the best physiological response and improvement of color quality and the glucose level was 23.00 ± 1.00 mg dL−1. The best color quality of the Sumatra barb is produced by exposure to the red light spectrum (M). Abstrak Ikan sumatra hasil budi daya petani belum mencapai kualitas warna yang cukup baik dibandingkan hasil tangkapan di alam. Salah satu penyebabnya adalah lingkungan pemeliharaan serta penangkaran ikan sumatra yang tidak sesuai. Penelitian ini bertujuan untuk mengevaluasi kualitas warna ikan sumatra Puntigrus tetrazona dengan paparan spek-trum cahaya berbeda pada media pemeliharaan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas enam perlakuan dan tiga ulangan yaitu kontrol (K), cahaya ruang (R), spektrum cahaya merah (M), spek-trum cahaya putih (P), spektrum cahaya biru (B), dan spektrum cahaya hijau (H). Metode yang digunakan untuk mengukur kualitas warna ikan sumatra yaitu perangkat lunak Photoshop CS 5 dan perhitungan sel kromatofora. Hasil analisis kualitas warna setelah 28 hari menggunakan perangkat lunak Photoshop CS5 menunjukkan perlakuan spek-trum cahaya merah (M) memiliki kualitas warna terbaik dengan persentase sebesar 48,81±1,57% untuk warna jingga dan 32,26±0,07% untuk warna hitam. Jumlah sel kromatofora tertinggi yaitu pada perlakuan spektrum cahaya merah (M) yaitu sebesar 147,3±3,7 sel mm-2. Spektrum cahaya merah (M) menunjukkan respons fisiologis dan peningkatan kualitas warna yang terbaik. Kadar glukosa pada perlakuan spektrum cahaya merah (M) yaitu sebesar 23,00±1,00 mg dL-1. Kualitas warna ikan sumatra Puntigrus tetrazona terbaik dihasilkan oleh paparan spektrum cahaya merah (M).

Color quality, behavioral response, and blood glucose levels of guppies Poecilia reticulata (Peters, 1859) with the addition of Indian almond leaves (Terminalia catappa) in fish containers Izhar Amirul Haq; Kukuh Nirmala; Yuni Puji Hastuti; Eddy Supriyono
Jurnal Iktiologi Indonesia Vol 22 No 1 (2022): February 2022
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v22i1.581

Guppies are ornamental fish that have economic value and can be cultivated. One of the problems in guppies cultivation is the poor quality of body color. The solution to this problem is to improve the environmental quality in guppy aquaculture. This study evaluates changes in color quality, behavioral response, and blood glucose levels of guppies using water immersion of Indian almond leaves on rearing media. Study was carried out using a completely randomized design consisting of five treatments. The rearing media was filled water with a total volume of 10 L from each treatment with different concentrations, namely, Control treatment (100% freshwater), A (75% freshwater + 25% Indian almond leaf water), B (50% freshwater + 50% Indian almond leaf), C (25% freshwater + 75% Indian almond leaf) and D (100% Indian almond leaf). Each treatment observed several test parameters such as color quality percentage, survival rate, chromatophores cells number, behavior, glucose levels, and the water physical-chemical parameters. Guppies' behavioral responses, in general, experienced an increasing change in each treatment for seven days. The highest color quality percentage by treatment D was 73.93±2.29%, and the highest glucose level was by the control treatment, which was 24.11±0.41 mg dL-1. The results of variance analysis showed that the water immersion of Indian almond leaves was significantly different on color quality, behavioral response, and blood glucose levels through Duncan's test (p<0.05). Abstrak Ikan guppy termasuk ikan hias yang memiliki nilai ekonomis dan dapat dibudidayakan. Salah satu masalah dalam budidaya ikan guppy yaitu kualitas warna tubuhnya yang tidak bagus. Upaya yang dapat dilakukan yaitu memperbaiki lingkungan budidaya ikan guppy. Studi ini bertujuan untuk mengevaluasi perubahan kualitas warna, respons tingkah laku, dan kadar glukosa ikan guppy menggunakan rendaman air daun ketapang pada media pemeliharaan. Evaluasi ini dilakukan dengan menggunakan rancangan acak lengkap yang terdiri atas lima perlakuan. Media pemeliharaan diisi air dengan total volume 10 L dari setiap perlakuan dengan konsentrasi yang berbeda yaitu, perlakuan Kontrol (100% air tawar), A (75% air tawar + 25% air daun ketapang), B (50% air tawar + 50% air daun ketapang), C (25% air tawar + 75% air daun ketapang) dan D (100% air daun ketapang). Setiap perlakuan diamati beberapa parameter uji seperti persentase kualitas warna, tingkat sintasan, jumlah sel kromatofora, tingkah laku, kadar glukosa, dan parameter fisik kimiawi perairan. Respons tingkah laku ikan guppy secara umum mengalami perubahan yang meningkat dalam setiap perlakuan selama tujuh hari. Persentase kualitas warna tertinggi pada perlakuan D yaitu sebesar 73,93±2,29% dan nilai kadar glukosa tertinggi pada perlakuan Kontrol yaitu 24,11±0,41 mg dL-1. Hasil analisis sidik ragam menunjukkan bahwa rendaman air daun ketapang berbeda nyata terhadap kualitas warna, respons tingkah laku dan kadar glukosa darah melalui uji Duncan (p0,05).

Micro/Nano Bubble Technology : Characteristics and Implications Biology Performance of Koi Cyprinus carpio in Recirculation Aquaculture System (RAS) Henry Kasmanhadi Saputra; Kukuh Nirmala; Eddy Supriyono; Nurul Taufiqu Rochman
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Technology for aquaculture is growing with the high demand of fish. Micro / nano bubble is one of technology that is developed to increase dissolved oxygen in water. This technology is used to increase Koi fish production in high density via RAS. The purpose of this study was to determine the characteristics of micro / nano bubble technology and its effect on biology of koi fish (Cyprinus carpio). Lutor was used to introduce micro / nano bubble in the fish tank by using fish length (e.g. 7.49 ± 0.29 cm). This research used completely randomized design with one factor (fish density) and three replications. This factor was three densities of fish at 15 fish.60 L-1 (A), 30 fish.60 L -1 (B), and 45 fish. 60 L -1 (C). The result showed that there was the absolute length of each treatments was (0.52 ± 0.03a) cm for A, treatment B (0.36 ± 0.07ab) cm and C treatment (0.29 ± 0.08b) cm. The best treatment is A treatment (15 fish. 60 L -1) and different significant in statistic with C treatment (45 fish. 60 L-1) but not significant in statistic with B treatment (30 fish. 60 L -1).

The Dynamics of Water Quality During Culture of Snakehead Fish (Channa striata) in The Aquarium Dewi Puspaningsih; Eddy Supriyono; Kukuh Nirmala; Iman Rusmana; Cecep Kusmana; Ani Widiyati
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Intensive culture of snakehead fish used artificial feed with high protein content. The impact of the high use of artificial feed will cause waste that can cause poor water quality and cause fish mortality. The purpose of this research was to know the dynamics of water quality during culture of snakehead fish Channa striata in the aquarium. The test animals used were snakehead fish with a length of 6.90 ± 0.49 cm and weight of 2.57 ± 0.59 g. Fish was maintenanced with a density of 2 fish L-1, 4 fish L-1 and 6 fish L-1. Fish was cultured for four days in an aquarium with size of 60x40x40 cm3 and water volume of 20 L. Water quality were observed, i.e, temperature, pH, dissolved oxygen, TAN, nitrite, nitrate, orthophosphate, hardness and alkalinity. The results showed that pH, temperature, hardness, TAN, nitrite, nitrate and ortophosphate were not significantly different between treatments (P>0.05), while dissolved oxygen and alkalinity were showed different significantly (P<0.05). On the fourth day of culture with a density of 4 fish L-1 and 6 fish L-1 has led to water saturation in the culture media. Dynamic of water quality was started on the third day of culture, whereas nitrites could not be converted to nitrate maximally. High levels of nitrate in the snakehead culture indicated that that this culture could be combined with an integrated aquaculture with plants.

CARBON TO NITROGEN RATIO AND NITROGENOUS WASTE ACCUMULATION IN THE INTENSIVE CATFISH (Clarias gariepinus) CULTURE Bambang Gunadi; Enang Harris; Eddy Supriyono; Sukenda Sukenda; Tatag Budiardi
Indonesian Aquaculture Journal Vol 6, No 1 (2011): (June 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

This experiment was carried out to determine the optimum C/N ratio for heterotrophic bacteria (biofloc) growth in order to control nitrogenous waste accumulation in the catfish (Clarias gariepinus) culture. Twenty fish with an initial individual size of about 50 g were stocked in fiberglass tanks which were filled with 200 L of water. Fish were fed with commercial floating fish feed with a protein level of 31%-33% (manufacturer label). The daily feeding rate was 2.5% of the fish biomass. The inoculation of commercial Bacillus sp. isolates was applied in the first day of the experiment after fish stocking in order to obtain a bacterial density in water of 106 cfu/L. Molases was suplemented daily to the tanks to adjust C/N ratio in water. Four C/N ratios, i.e. 0, 7, 14, and 21, were applied as treatments in this experiment. The results showed that molasses suplementation up to C/N ratio 14 to 21 were able to support the growth of heterotrophic bacteria and to inhibit the accumulation of total ammonia nitrogen (TAN) and nitrite in water therefore increase water quality for better growth of cultured catfish.

TOKSISITAS AKUT NONILPHENOL PADA STADIA AWAL IKAN NILA, Oreochromis niloticus (Linnaeus, 1758) DAN IKAN KOMET, Carassius auratus (Linnaeus, 1758) Muhamad Yamin; Eddy Supriyono; Kukuh Nirmala; Muhammad Zairin Jr.; Enang Haris; Riani Rahmawati
Jurnal Riset Akuakultur Vol 12, No 1 (2017): (Maret 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Ikan nila (Oreochromis niloticus) dan ikan komet (Carassius auratus) adalah komoditas ikan konsumsi dan ikan hias air tawar yang paling banyak dibudidayakan masyarakat Indonesia. Namun keberadaan bahan pencemar seperti nonilphenol dapat mengancam produktivitas kegiatan budidaya ikan tersebut karena dapat menyebabkan gangguan perkembangan bahkan kematian khususnya pada tahap awal perkembangan ikan (early development stage). Penelitian ini bertujuan untuk mengetahui tingkat toksisitas akut median lethal concentration (LC50) nonilphenol pada larva ikan nila dan ikan komet. Penelitian dilakukan di laboratorium Balai Penelitian dan Pengembangan Budidaya Ikan Hias (BPPBIH) Depok. Penelitian terdiri atas uji mencari nilai kisaran (range finding test/RFT) dan dilanjutkan dengan uji akut. Level konsentrasi nonilphenol diatur berdasarkan deret logaritmik di mana untuk RFT menggunakan konsentrasi 0,01; 0,10; dan 1,00 mg/L; sedangkan level konsentrasi nonilphenol untuk uji akut ditentukan dari hasil RFT. Hasil analisis probit menujukkan nilai LC50 nonilphenol pada jam ke-96 pada larva ikan nila dan ikan komet berturut-turut berada pada konsentrasi nonilphenol 0,33 dan 0.10 mg/L. Sementara kematian 100% (LC100) larva ikan nila dan ikan komet pada jam ke-96 masing-masing berada pada konsentrasi 0,61 dan 0,50 mg/L. Merujuk pada kriteria toksisitas bahan dari Komisi Pestisida Departemen Pertanian, maka hasil penelitian ini menunjukkan bahwa nonilphenol tergolong dalam bahan berbahaya dengan daya racun yang sangat tinggi.Nile tilapia (Oreochromis niloticus) and comet goldfish (Carassius auratus) are the major fresh water fish commodities in Indonesia used for both consumption and ornamental fish. However, production of the fish threatened by the presence of nonylphenol which can interfere with early development stage. Research objectives were to evaluate acute toxicity of nonylphenol to larval of nile tilapia and comet goldfish and to compare median lethal concentration (LC50). Research was carried out in the RDIOF, Depok. Experiments consisted of range finding test/RFT and accute test. Nonylphenol concentrations of RFT were 0.01, 0.10, and 1.00 mg/L. The results showed that LC50-96 hours of tilapia and comet were 0.33 and 0.10 mg/L respectivelly. Total mortality (LC100-96 hours) for tilapia and comet were 0.61 and 0.50 mg/L respectivelly. These results of nonylphenol concentrations, according to toxic level criteria by The Department of Agriculture’s Pesticide Commission, is categorized as dangerous goods with very high level of toxicity.

EVALUASI PENGGUNAAN JENIS SELTER BERBEDA TERHADAP RESPONS STRES DAN KINERJA PRODUKSI PENDEDERAN LOBSTER AIR TAWAR Cherax quadricarinatus DALAM SISTEM RESIRKULASI Arif Faisal Siburian; Kukuh Nirmala; Eddy Supriyono
Jurnal Riset Akuakultur Vol 13, No 4 (2018): (Desember 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Sintasan yang rendah pada pembenihan lobster air tawar tidak terlepas dari karakteristik lobster air tawar yang teritorial pada areal yang terbatas, sering menunjukkan sifat agresif pada umur muda, dan memiliki perilaku kanibalisme. Tujuan dari penelitian ini adalah mengevaluasi pengaruh penggunaan selter yang berbeda terhadap respons stres dan kinerja produksi sehingga dapat menentukan jenis selter yang tepat untuk pendederan lobster air tawar Cherax quadricarinatus. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Perlakuan pada penelitian ini adalah penggunaan selter yang berbeda yakni pipa PVC, roster (ventilasi blok), tali rafia, dan tanpa selter (kontrol). Benih lobster air tawar yang digunakan memiliki bobot rata-rata awal berkisar antara 0,60±0,09-0,64±0,02 g dan panjang total rata-rata awal berkisar antara 2,55±0,06-2,61±0,03 cm yang dipelihara dalam sistem resirkulasi selama 60 hari. Perlakuan dengan penggunaan selter ataupun tanpa selter (kontrol) tidak memberikan pengaruh signifikan (P>0,05) terhadap respons stres, namun memberikan pengaruh signifikan (P<0,05) pada kinerja produksi benih lobster air tawar di akhir penelitian. Perlakuan terbaik pada penelitian ini adalah penggunaan selter tali rafia dengan kadar glukosa sebesar 101,00±17,35 mg/dL; protein total sebesar 5,00±0,36 g/dL; sintasan sebesar 86,67± 0,00%; bobot rata-rata akhir sebesar 2,86-3,46 g; panjang total rata-rata akhir sebesar 4,47-5,08 cm; laju pertumbuhan bobot spesifik sebesar 2,92±0,21%/hari; laju pertumbuhan panjang spesifik sebesar 1,15±0,08%/hari; rasio konversi pakan sebesar 2,97±0,05; dan biomassa total sebesar 45,02±1,10 g. Penggunaan tali rafia sebagai selter menjadi perlakuan yang terbaik karena kemampuan tali rafia memisahkan banyak individu sehingga dapat mengurangi kanibalisme dengan cara meminimalkan kontak antarbenih lobster air tawar.The low survival rate in seed production of freshwater crayfish is mainly caused by the territorial behavior of freshwater crayfish which leads to aggressiveness and cannibalism behavior even at a young age. This research aimed to determine the effect of using different nursery shelters on stress responses and production performance of freshwater crayfish Cherax quadricarinatus. This research used a completely randomized design consisted of four treatments, each with triplicate. The treatments used were different shelters made from PVC pipes, ventilation blocks, raffia ropes, and no shelters as controls. The freshwater crayfish seeds had initial weights ranged from 0.60±0.09-0.64±0.02 g, and total length ranged from 2.55±0.06-2.61±0.03 and reared in a recirculation system for 60 days. The results of the research showed that all treatments including controls did not have a significant effect (P>0.05) on stress responses but had a significant effect (P<0.05) on the production performance of freshwater crayfish seed at the end of this research. The seeds reared with raffia ropes shelter had the best production performance indicated by its glucose level of 101.00±17.35 mg dL1, total protein level of 5.00±0.36 g dL1, survival rate of 86.67 ± 0.00%, final average weight of 3.46 ± 0.08 g, final average total length of 5.08 ± 0.12 cm, specific weight growth rate of 2.92±0.21% per day, specific length growth rate of 1.15± 0.08% per day, feed conversion ratio of 2.97±0.05, and total biomass of 45.02±1.10 g. The use of raffia ropes is considered as the best shelter for freshwater crayfish seed as it provides more space to separate individual seeds which can reduce cannibalism behaviour due to minimum contact between individual seeds.

RESPONS FISIOLOGIS DAN KINERJA PERTUMBUHAN IKAN NILA PADA MEDIA RENDAH AMONIA DAN DIBERI SUPLEMEN ASAM GLUTAMAT Titin Kurniasih; Dedi Jusadi; Muhammad Agus Suprayudi; Sri Nuryati; Muhammad Zairin Jr.; Eddy Supriyono
Jurnal Riset Akuakultur Vol 15, No 3 (2020): (September, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Ketika dipapar media tinggi amonia, ikan nila mengalami perubahan metabolisme asam amino yang cukup signifikan, dan suplementasi asam glutamat berguna untuk memperbaiki perubahan yang merugikan akibat paparan amonia. Akan tetapi informasi mengenai aspek metabolisme asam amino pada ikan nila yang dipapar amonia rendah masih sangat terbatas. Penelitian ini dilakukan untuk mengevaluasi pengaruh suplementasi asam glutamat pada ikan nila merah yang dipelihara pada media budidaya rendah amonia terhadap respons fisiologis dan kinerja pertumbuhan. Ikan nila dengan bobot rata-rata 9,97 ± 0,38 g ditebar sebanyak 20 ekor pada setiap akuarium (padat tebar 1,0 g L-1). Empat jenis pakan isoprotein (kadar protein 28%) dan isoenergi (4245 ± 22,48 kkal kg-1) disuplementasi asam glutamat masing-masing sebanyak 0% (Glu 0), 0,75% (Glu 0,75), 1,5% (Glu 1,5) dan 2,25% (Glu 2,25). Setiap perlakuan diberi empat ulangan. Penelitian ini dilakukan selama 60 hari. Hasil penelitian menunjukkan bahwa pemberian pakan yang ditambah glutamat memberi efek pada respon fisiologis ikan. Aktivitas enzim aspartate aminotransferase (AST) pada Glu 2,25 lebih rendah dibandingkan dengan perlakuan lainnya, yang menjadi indikasi penurunan beban kerja hati. Ada kecenderungan peningkatan kadar aspartat, alanin, leusin, isoleusin dan valin pada jaringan hati seiring dengan meningkatnya kadar suplementasi asam glutamat. Di dalam penelitian ini, kinerja pertumbuhan ikan tidak berbeda nyata antar perlakuan. Kesimpulannya adalah bahwa konsumsi pakan yang ditambah asam glutamat 2,25% mampu memperbaiki respons fisiologis ikan akibat menurunnya beban kerja hati yang dicirikan dengan penurunan nilai AST, serta meningkatnya kandungan beberapa asam amino hati, walau belum mampu memperbaiki kinerja pertumbuhan dan pemanfaatan pakan oleh ikan nila. Informasi ini berguna untuk pengembangan riset terkait aspek metabolisme asam amino pada ikan nila yang terpapar media tinggi amonia.When exposed to high ammonia aquatic environment, nile tilapia experienced a significant change in hepatic amino acid metabolism and glutamic acid supplementation can reduce the effects of the adverse change. However, there are no sufficient information on the amino acid metabolisme of tilapia exposed to low environmental ammonia. This research was performed to evaluate the effects of oral supplementation of glutamic acid on the aminotransferase enzymes activity and growth performance of red tilapia reared in low environmental ammonia (LEA) with NH4 concentration of 0.10 mg L-1. Fish with an average weight of 9.97 ± 0.38 g were stocked with an initial rearing density of 1.0 g L-1(20 fish in each aquarium). Four isonitrogenous (crude protein 28%) and isocaloric (4246 ± 22.48kcal kg-1) experimental diets were prepared with supplementation of different ratios of glutamic acid at 0% (Glu0), 0.75% (Glu0.75), 1.5%(Glu1.5) and 2.25 % (Glu2.25) to feed, respectively. All treatment groups were arranged quadruplicate. Fish were fed with the diets for 60 days. The results showed that the supplementation of glutamic acid in the diet affected the physiological response of the fish. The aspartate aminotransferase (AST) activity of Glu2.25 was significantly lower compared to that of the other treatments, which indicated a decrease in liver workload. There is a tendency of increased levels of hepatic free aspartate, alanine, leucine, isoleucine, and valine following the increase of glutamic acid supplementation level. The fish growth performance was insignificantly different between the treatments. It is concluded that a diet supplemented with 2.25% of glutamic acid could improve the physiological response of red tilapia, although no significant growth improvement should be expected. These research finding could serve as an important basic information for future research on amino acid and endogenous ammonia metabolism in nile tilapia exposed to high ammonia aquatic environment.

Co-Authors . Sukenda . Sulistiono Adang Saputra Adang Saputra Adianto, Asep Agustinus Ngaddi Ahmad Ghufron Mustofa Ahmad Maksum Aisyah Lukmini Alexander Burhani Marda, Alexander Burhani Ali Djamhuri Amin Pamungkas Anang Hari Kristanto Ani Widiyati Ani Widiyati Anwar, Rifky Alwafi Any Widiyati Ardyen Saputra, Ardyen Arif Faisal Siburian Aris Darmansah Aris Darmansah Asep Rachmat Pratama Bambang Gunadi Bambang Gunadi Bambang Gunadi Bambang Priyo Utomo Berlianti . Budiyanti Cecep Kusmana Chrisliana, Chrisliana Dadang Shaffruddin Dadang Shafruddin Dadang Shafruddin Dadang Shafrudin Daniel Djokosetianto Daniel Djokosetianto Daniel Djokosetiyanto Darmawan, Ahmad Rumi DEDI JUSADI Dedi Pardiansyah Dewi Puspaningsih Diana Sriwisuda Putri Diana Sriwisuda Putri, Diana Sriwisuda Diki, Diki Dinamella Wahjuningrum Dinar Tri Soelistyowati Dini Wulandari Dody Sihono Donny Prariska Ee Ling, Yong Eka Rosyida Eko Harianto, Eko Enang H. Surawidjaja Enang Haris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja Ernik Yuliana Eva Prasetiyono Failu, Ismail Faturochman, Ilman Fauziah Azmi Ferdinand Hukama Taqwa Fina Lestari Guttifera Hamim Hamim Hamzah, Aris Sando Hanif Azhara, Muhammad Harton Arfah Hendriana, Andri Humairani, Humairani I Wayan Nurjaya Idil Ardi Ima Kusumanti Iman Rusmana Iman Sari Lubis, Vina Imron Imron, Imron Ing Mokoginta Intan Wulandari Irzal Effendi Izhar Amirul Haq I’ana Rahma Salisa Jariyah, I’it Rohmatul Julie Ekasari Kukuh Adiyana Kukuh Adiyana Kukuh Adiyana Kukuh Adiyana Kukuh Adiyana Kukuh Adiyana, Kukuh Kukuh Nirmala Kukuh Nirmala Lesmana, Dudi Lies Setijaningsih Lila Antara, Kadek Lilik Sulistyowati Lina Warlina Listyarini, Sri Liubana, Debora Victoria Lolita Thesiana Lolita Thesiana M. Faisol Riza Ghozali M. Toelihere M. Yusuf Arifin M. Zairin Junior Maman Tocharman Mariam, Susanti Mariska Putri Nur Hidayah Mas Tri Djoko Sunarno Melati, Aulia Firda Mia Setiawati Moh. Burhanuddin Mahmud Muh. Saleh Nurdin Muhamad Dzikri Muhamad Yamin Muhamad Yamin MUHAMMAD AGUS SUPRAYUDI Muhammad Fauzan Isma Muhammad Nabil Muhammad Saifuddin Muhammad Zairin Jr. Muhammad Zairin Jr. Mulyasari Mulyasari MUNTI YUHANA MURIE DWIYANITI1 N Hutomo N. Potalangi Nana Ganda Neltje Nobertine Palinggi Neltje Nobertine Palinggi Nur Fauziyah Nur Hasanah Nuradzani, Daffa Nurul Taufiqu Rochman Nurul Taufiqu Taufiqu Rochman O.D. Subakti Hasan Obed Lepa Saba Kulla Odang Carman Permatasari, Sheny Petrus Rani Pong-Masak Prama, Ega Aditya Pras, Eva Prasetiyono Pratama, Asep Rachmat Puji Hastuti, Yuni Rahma Vida Anandasari, Rahma Vida Rasul Raudhatus Sa'adah Revfvi Al Ghaney Rizal Riandini Riandini Riani Rahmawati Richard Latuny Ridwan Affandi RIDWAN AFFANDI Rifqah Pratiwi Rio Yusufi Subhan Rirojoyo, Gerald P P Ris Dewi Novita Rizki Eka Puteri Rohman Rohman Ruspindo Syahputra S. Hastuti Sabilu, Kadir Sabilu, Murni Santi Febrianti Santosa Koesoemadinata Saputra, Henry Kasmanhadi Selly Ratna Sari Septya, Saka Tirta Setijaningsih, Lies Sihananto, Bambang Siska Mellisa Solly Aryza Sophia N. M. Fendjalang Sri Nuryati Sri Wahyuni Firman Sugeng Budiharsono Sugeng H. Suseno Suhaiba Djai Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sulistiono Supriani Suri Purnama Febri Susanti Mariam Suseno, Sugeng Hari Sutrisno Sutrisno Tatag Budiardi Teuku Fadlon Haser Thomas Nugroho Titin Kurniasih Tri Heru Prihadi Usman Usman Usman Usman Usman Usman Wa Iba, Wa Iba Wahyu Pamungkas Wahyu Wahyu Wasjan Wasjan WIDANARNI WIDANARNI Widiyati, Any Wijianto Wijianto Wildan Nurussalam Wirantari, Ayu Puspa Wisriati Lasima Y. Hadiroseyani Yosmaniar Yosmaniar Yosmaniar Yosmaniar Yosmaniar Yosmaniar Yoyo Wiramiharja Yuni Puji Hastuti Yuni Puji Hastuti Yuni Puji Hastuti

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