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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Buletin Peternakan Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal Indonesian Journal for Health Sciences (IJHS) Jurnal Teknologi Informasi dan Pendidikan Value: Jurnal Manajemen dan Akuntansi Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Intervensi Komunitas: Jurnal Pengabdian Masyarakat Jurnal Kesehatan: Jurnal Ilmu- Ilmu Keperawatan, Kebidanan, Farmasi dan Analis Kesehatan Jurnal Pendidikan Guru (JPG) Komunika: Journal of Communication Science and Islamic Dakwah Bima Journal : Business, Management and Accounting Journal Educenter: Jurnal Ilmiah Pendidikan Idarah Tarbawiyah: Journal of Management in Islamic Education GENIUS JOURNAL (General Nursing Science Journal) At Tawasul: Jurnal Komunikasi dan Penyiaran Islam Jurnal Komputer Teknologi Informasi Sistem Komputer (JUKTISI) Jurnal VNUS (Vocational Nursing Science) Jurnal Riset Rumpun Ilmu Bahasa (JURRIBAH) Aurelia: Jurnal Penelitian dan Pengabdian Masyarakat Indonesia KOLONI KIAN Journal Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Journal of Educational Management Research Nurul Ilmi : Journal of Health Sciences and Midwifery Kriez Academy Jesocin : Journal of Jabar Economic Society Networking Forum RESLAJ: Religion Education Social Laa Roiba Journal Mimbar Kampus: Jurnal Pendidikan dan Agama Islam Jesocin : Journal of Economics, Accounting, Business, Management, Engineering and Society Jurnal Multidisiplin Indonesia Maneggio Journal of Community Service (Abdisci) JER EDUCENTER JURNAL PENDIDIKAN Jurnal Agripet Animal Production Transformasi Manageria: Journal of Islamic Education Management JIS

Asep Gunawan

Departement Of Animal Production And Technology, Faculty Of Animal Science, IPB University, Jalan Agatis, Kampus IPB Dramaga Bogor 16680, Indonesia

Author-ID : 2627538

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Identification of Single Nucleotide Polymorphism and Pathway Analysis of Apolipoprotein A5 (APOA5) Related to Fatty Acid Traits in Indonesian Sheep A. Gunawan; D. Anggrela; K. Listyarini; M. A. Abuzahra; Jakaria Jakaria; M. Yamin; I. Inounu; C. Sumantri
Tropical Animal Science Journal Vol. 41 No. 3 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was aimed to identify single nucleotide polymorphism (SNP) and pathway analysis of APOA5 with fatty acids traits in sheep. A total of 47 rams consisted of 20 heads of Javanese Fat Tailed (JFT), 17 heads of Javanese Thin Tailed (JTT), and 10 heads of Garut Composite Sheep (GCS) were used in this study. Fatty acids traits were measured at the age of 12 months with the average body weight of 25-30 kg. Identification of polymorphism of APOA5 (g.26929941 C>T) gene were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The pathway analysis of APOA5 gene was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The SNP of APOA5 gene were found polymorphic with three genotypes (CC, CT, and TT). The gene frequency of CC, CT, and TT were 0.83, 0.11, and 0.06, respectively. The chi square test revealed that the locus of APOA5 (g.26929941 C>T) was in Hardy-Weinberg equilibrium, except in thin tailed sheep. The chi-square values of JFT, JTT, and GCS were 0.05, 0.03, and 0.04, respectively. A SNP of APOA5 was associated (P<0.05) with polyunsaturated fatty acids including eicosapentanoic acid (C20:5n3) and docosahexanoic (C22:6n3) and saturated fatty acid lauric acid (C12:0) in combined population (JFT, JTT, and GCS). Furthermore, pathway analysis showed that APOA5 belonged to phagosome and peroxisome proliferator-activated receptors (PPAR) signaling pathway. In conclusion, this analysis has identified APOA5 and related pathway crucial for fatty acid composition and metabolism in sheep, as well as this gene provide molecular marker to select sheepmeat with high unsaturated fatty acid.

Polymorphisms and Associations of the NRAMP-1 and iNOS Genes on Newcastle Disease and Salmonella enteritidis Resistances in SenSi-1 Agrinak Chickens M. Ardiyana; A. Gunawan; S. Murtini; T. Sartika; C. Sumantri
Tropical Animal Science Journal Vol. 43 No. 2 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

NRAMP-1 and iNOS genes were reported to be associated with a defense mechanism against bacteria and virus infections. This study aimed to identify NRAMP-1 and iNOS genes polymorphisms and their associations with the defense mechanisms against Salmonella enteritidis and Newcastle Disease (ND) in SenSi-1 Agrinak chicken. The present study used a total number of 172 SenSi-1 Agrinak chicken. Identifications of NRAMP-1 and iNOS genes polymorphisms were performed by PCR-RFLP method. NRAMP-1 and iNOS genotypes were associated with immunoglobulin Y (IgY) concentration, specific antibodies against S. enteritidis and ND using General Linear Model (GLM). Immunity characteristics were further grouped into high, medium, and low categories. NRAMP-1|SacI exon 11 and iNOS|AluI intron 24 in SenSi-1 Agrinak chickens were polymorphic. TC genotype has a higher immune response to infectious agents compared to TT and CC genotypes. The frequency of C allele was higher than the T allele in the concentration of immunoglobulin Y (IgY), antibodies titers against S enteritidis and ND. The TC genotype of NRAMP-1 gene was significantly associated with ND antibody titers, and the TT genotype of iNOS was significantly associated with S. enteritidis specific antibody. NRAMP-1 and iNOS genes can be used as potential candidate genes for immune traits in SenSi-1 Agrinak chickens.

Genetic Variation of Eight Indonesian Swamp-Buffalo Populations Based on Cytochrome b Gene Marker M. Rusdin; D. D. Solihin; A. Gunawan; C. Talib; C. Sumantri
Tropical Animal Science Journal Vol. 43 No. 1 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Genetic variation is a major concern in animal genetic resources conservation program. This study aimed to analyze genetic variation and phylogeography of eight Indonesian swamp-buffalo populations based on cytochrome b gene marker. A total of 78 DNA fragment samples originating from eight Indonesian swamp-buffalo populations were used in this study, namely Bombana Island, Bombana mainland, Kolaka, Konawe, North Toraja, West Nusa Tenggara, Banten, and Aceh with 11, 10, 13, 14, 10, 10, 5, and 5 samples, respectively. The cytochrome b gene sequence and genetic variation parameters were analyzed in MEGA software (ver 6), and DnaSP software (ver 5.10.01). The results of this study showed that all DNA-fragment samples were successfully amplified by PCR technique with the size target (906 bp). Based on the distribution of all samples, it was found 9 polymorphic sites, and 10 haplotypes with the haplotype diversities were 0.6590. The average of genetic distances between populations ranged from 0.0000-0.002. They were grouped into two main clusters. The first cluster consisted of Aceh, North Toraja, West Nusa Tenggara, Banten, Kolaka, and Konawe populations, meanwhile, the second cluster consisted of Bombana Island, Bombana mainland, Kolaka, and Konawe populations. The results of the study were concluded that eight Indonesian local swamp-buffalo populations were grouped into two main clusters where Bombana Island and Bombana mainland populations were specific breeds because they were only found in the second cluster and also had specific nucleotides sites on the 57 nucleotides which C base changed to T. The results of this study were useful in formulating the program of conservation and utilization of Indonesian buffalo genetic resources, especially in the buffalo population with specific breeds.

Polymorphism of L-FABP (SNP g. 1593 C>T) Gene and Its Association with Fatty Acid Composition, Carcass, and Meat Quality in Cihateup Duck A. S. Mentari; A. Gunawan; R. R. Noor
Tropical Animal Science Journal Vol. 43 No. 2 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Fatty acid composition, carcass, and meat quality are quantitative traits which are controlled by several genes or polygenic. One of the genes that plays important role in the fatty acid in meat quality and fatty acid composition is liver-type fatty acid-binding protein (L-FABP). The aims of this study were to identify single nucleotide polymorphism (SNP) of liver-fatty acid binding protein (L-FABP) gene and its association with fatty acid, carcass, and meat quality traits in cihateup duck. Cihateup ducks were originated from Tasikmalaya, West Java, Indonesia. The study used a total of 98 ducks aged of 12 weeks old with the average body size of 1.4±0.12 kg for PCR-RFLP analyses and 76 ducks for the association analyses. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was used to identify SNP g. 1593 (C>T) of L-FABP gene. The associations of L-FABP genotypes with fatty acid, carcass, and meat quality traits were performed using T-test procedures. The result showed that the SNP of L-FABP gene was polymorphic with three genotypes (CC, CT, and TT). The Chi-square test revealed that the locus of L-FABP (g. 1593 C>T) was in Hardy - Weinberg equilibrium. L-FABP gene was significantly associated (p<0.01) with the carcass portions, providing neck and percentage of neck as well as significantly associated (p<0.05) with saturated fatty acids, i.e., lauric acid (C 12:0) and palmitic acid (C 16:0); polyunsaturated fatty acid, i.e., eicosadienoic acid (C 20:2); carcass portions, i.e. neck and percentage of neck. The SNP g. 1593 (C>T) of L-FABP gene may be a useful marker for selecting and producing duck meat having desirable fatty acids and carcass and meat quality.

Polymorphism of CD1B Gene and Its Association with Yolk Immunoglobulin (IgY) Concentration and Newcastle Disease Antibody Titer in IPB-D1 Chicken M. F. Al-Habib; S. Murtini; A. Gunawan; N. Ulupi; C. Sumantri
Tropical Animal Science Journal Vol. 43 No. 3 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2020.43.3.197

The CD1B gene has an important role in the immune system of poultry by mediating antibody induction. The study aimed to identify the CD1B gene polymorphism and its association with the concentration of IgY and ND antibody titers in IPB-D1 chicken. As many as 111 of IPB-D1 chickens at 21 weeks old were used in this study. Polymorphism identification of the CD1B gene was made using the PCR Sequencing method, while the IgY and ND antibody titers were done using the ELISA and HI test, respectively. The associations of gene polymorphism with IgY and ND antibody titers were analyzed using the General Linear Model (GLM) procedure and Duncan`s Multiple Range test. The results show that there are 4 SNPs in exon 3, i.e., c.550 G>A, c.562 T>A, c.588 A>G, and c.612 C>G. All the SNPs are missense, silent mutations, and polymorphic. The c.550 G>A and c.562 T>A SNPs were in Hardy Weinberg’s equilibrium and heterozygosity (0.054-0.252) condition, while the c.588 A>G and c.612 C>G SNPs were not in equilibrium and their heterozygosity was low (0.072-0.252). The combination of 4 SNPs generated 8 haplotypes, i.e., haplotypes 1, 2, 3, 4, 5, 6, 7, and 8. Haplotypes 1, 2, and 8 had high frequencies (17.6%-23.5%). The c.588 A>G and c.612 C>G mutations were significantly associated (p<0.05) with IgY concentration and c.562 T>A were significantly associated (p<0.05) with ND antibody titers. The haplotypes 2 and 8 with a combination of c.588 A>G and c.612 C>G mutations had higher IgY concentration and ND antibody titers (p<0.05) compared to the other haplotypes. In conclusion, this study has identified the CD1B gene as a polymorphic and is associated with IgY concentration and ND antibody titers in IPB-D1 chicken.

Co-Authors A. Furqon A. S. Mentari Ahmad Furqon Akbanugra Yudhananda Akhmad Solihin Alfadhilah, Azzahra Ali Mulyanto aliyah, siti nur Amri, Fisma Andini, Diong Anneke Anggraeni Astira, Adinda Wanda Ayu Safitri, Nita Bintari Janatunnisa, Meida C Sumantri C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. Talib Cahyo Budiman Cece Sumanri Cece Sumantri Chalid Talib Chandra Anugrah Putra D. Anggrela D. D. Solihin D.A.R, Deni Dedi Duryadi Solihin Dedi Duryadi Solihin Depison Depison Depison Depison Desi Hasbiyah Deswani Deswani DEWI APRI ASTUTI Dewi, Yuli Sartika Yuli Diana Sari, Deuis Dwi Mulyani, Mutia Edi Kusmayadi Elmy mariana F Hasan Falizar Rivani Fatmawati, Ariani Febriyansyah, Hevy Fidha, Rofiqotul Firmansyah, Adit Gushairiyanto Gushairiyanto Hadi Harisuddin, Abdul Hanum Isfaeni Heksa putra , Dadang Heni Marliany Herpina Herpina Himmatul Khasanah I. Inounu Ikhtiono, Gunawan Ima Sukmawati, Ima Indriya Indry Yunizul Pesha Irpan Ali Rahman Iskandar, Rhoma Ismeth Inounu Istikomah, Hana Ita Djuwitaningsih J. P. Munyaneza Jakaria Jakaria Jamil, Seli Nur Fatimah Jaya, Suhana Minah Junaedi Junaedi K. Listyarini K. Listyarini Katrin Roosita Komalasari, Iin KURNIAWAN, SYAHRUL FAUZI Kusnadidi Subekti Linda Oktavianingsih Listyarini, Kasita Luse Tokan, Alvares M. A. Abuzahra M. Ardiyana M. F. Al-Habib M. F. Ulum M. J. Uddin M. Yamin Ma'arif, Naufal MARIA ULFAH Maulidina, Azzahra Mohamad Yamin Mohamad Yamin Mubarak, Zahid Muh. Rusdin Muhamad Azhar Alwahid Muhammad Andi Setiawan Muhammad Suhendra, Muhammad Mulmuttaqin, M. Imam N. Ulupi Nawawi, M. Kholil niati, Rachma Nia Niken Ulupi Noneng Siti Rosidah NOVIANTI, ELIS Novita Nur Anggraeni Nur Amalina, Nadilah Nur Fauziah, Diny Nur Hidayat Nur'apipah, Pipih Palan Taran, Arsyifa Pamungkas, Fandy Pesha, Indry Nirma Yunizul Pratama, Bondan Prayitno, Arief Prayitno, Eka Bagus Priyanto Priyanto Priyono, Dadang Purnama, Dwi Rahmawati Putri , Nendela Resta Angga Putri Ria Angelina Putri, Wulan Cahya Putriani, Siti Nurmah Qhamarullah, Ariefuddin R R Noor R. Iis Arifiantini R. Priyanto R. Putri R. R. Noor R. R. Noor Rahmah, Amelia Azizah Rahmalia, Novita Rahmasuli, Resa Ramadhani, Hendriansah Ranawigena, Ghaitsa Ratna Ratna Ratna Sholatia Harahap Raudah Putri Ekawati RH Mulyono, RH Risman, Helda Rivani, Falizar Robbani, Muhammad Fikri Rofiah Ronnie Permana Ronny Rachman Noor Rudi Afnan Rudi Kurniawan Rudy Priyanto S. Murtini S. Murtini S. Y. Basril Saepudin, Didin Septiyawan, Ganda Adi Shidiq, Fajrin Siti Afifah Solihah, Rosidah SRI RAHAYU Suhanda Suhanda , Suhanda Sulistiarini, Sulistiarini Sutisna Sutisna SYAMSUDAR, BANGBANG Syarifah Gustiawati Mukri Sya’bana, Muhamad Rizki T. Sartika Tajudin, Ahmad Tiara Ningrum, Putri Azizah Tohir Solehudin Tri Rochmadi Tuti Suryati Utama, Muhammad Alif Wicaksono , Yanuar Widya Pintaka Bayu Putra Woki Bilyaro Yanuar, Irfan Yono, Yono Yudi Wahyudin Suwandi Yuliananda, Devi Yulianto Yulianto Yun Alwi Yusuf Safari Yusuf, Salman Zaenal Aripin Zakiyah Harsyah, Fathiyah

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