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All Journal Politika: Jurnal Ilmu Politik ELKHA : Jurnal Teknik Elektro Hunafa: Jurnal Studia Islamika EXPOSURE JOURNAL Jurnal Dimensi BERITA BIOLOGI Anglo-Saxon : Jurnal Ilmiah Program Studi Pendidikan Bahasa Inggris JPPUMA: Jurnal Ilmu Pemerintahan dan Sosial Politik Universitas Medan Area AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat MANAJERIAL Risâlah, Jurnal Pendidikan dan Studi Islam JTERA (Jurnal Teknologi Rekayasa) Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Akuatiklestari Jurnal Media Elektro : Journal of Electrical Power, Informatics, Telecommunication, Electronics, Computer and Control System Journal of Humanities and Social Studies Musawa: Journal For Gender Studies Journal of Education, Humaniora and Social Sciences (JEHSS) DIKEMAS (Jurnal Pengabdian Kepada Masyarakat) JEECAE (Journal of Electrical, Electronics, Control, and Automotive Engineering) JIKAP PGSD: Jurnal Ilmiah Ilmu Kependidikan Jurnal Fokus Elektroda : Energi Listrik, Telekomunikasi, Komputer, Elektronika dan Kendali Edum Journal Dinasti International Journal of Management Science PERSPEKTIF Jurnal Akuakultura Universitas Teuku Umar SOSIOEDUKASI : JURNAL ILMIAH ILMU PENDIDIKAN DAN SOSIAL Strukturasi: Jurnal Ilmiah Magister Administrasi Publik Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences G-Couns: Jurnal Bimbingan dan Konseling Journal La Sociale Waniambey: Journal of Islamic Education JCP (Jurnal Cahaya Pendidikan) Fakultas Keguruan dan Ilmu Pendidikan GALENICAL : Jurnal Kedokteran dan Kesehatan Mahasiswa Malikussaleh International Journal of Contemporary Islamic Law and Society Altifani : Jurnal Pengabdian Masyarakat Ushuluddin, Adab, dan Dakwah Jurnal TLM Blood Smear Journal of Public Representative and Society Provision Proceeding Applied Business and Engineering Conference Jurnal Ilmiah Administrasi Publik dan Pemerintahan E-JRM PharmaCine : Journal of Pharmacy, Medical and Health Science Inovbiz: Jurnal Inovasi Bisnis Seri Manajemen, Investasi dan Kewirausahaan Journal of Law, Education and Business FASKES : Jurnal Farmasi, Kesehatan, dan Sains Cakrawala Ekonomi dan Keuangan Journal of Leadership, Management and Policy in Education Deka in Medicine International Journal of Research in Counseling JOURNAL OF ISLAMIC COMMUNITY AND DEVELOPMENT Jurnal Integrasi Manajemen Pendidikan Panicgogy International Journal Journal of Multidiscipline and Collaboration Research TANJAK : Jurnal Pengabdian Kepada Masyarakat Auxilium : Jurnal Pengabdian Kesehatan Frontiers in Sustainable Science and Technology
Adam Adam
Penangkapan Ikan, Politani Negeri Pangkep
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Journal : BERITA BIOLOGI

 1 
EFEK FERRI SITRAT TERHADAP KEMAMPUAN KHAMIR Candida tropicalis DALAM MEREDUKSI 3-(4,5-DIMETHYLTHIAZOL-2-YL)- 2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) [Effect of ferric citrate on 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) reduction in yeast Candida tropicalis] Julistiono, Heddy; Muthmainah, Resti Siti; Adam, Adam
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.416 KB) | DOI: 10.14203/beritabiologi.v11i2.488

Abstract

Effect of iron (ferric citrate) on 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) reduction in yeast Candida tropicalis was investigated. Reduction of MTT in yeast grown in YMB media containing 5 mM of ferric citrate decreased significantly compared to that of yeast grown containing 0, or 1.25 or 2.5 mM ferric citrate after 24 h incubation. However, there was no difference in cell density among cultures treated with 0 mM, 1.25 mM, and 5 mM ferric citrate. Ferric citrate of 5 mM caused smaller colony when cells were grown on YPDG media. Reduction of MTT in smaller colony cells was weaker than that of with normal size colony. An antioxidant, Epigallocatechin Gallate (EGCG) of 0.01 % could not reverse MTT reduction caused by 5 mM ferric citrate. Since enzymes responsible in MTT reduction are usually located in mitochondrion, the data suggested that, in the condition of 5 mM ferric citrate might cause mitochondrion disorder without killing the yeast cells. The data was in concordance with other studies on other yeast or human cells. However, this study does not show role of free radicals provoked by high level of iron concentration.
Co-Authors . Zulfan A.R. Pratiwi Hasanuddin AA Sudharmawan, AA Abubakar Abubakar Ainal Mardhiah Akmal, Andi Al-Adawiyah, Rabi'ah Albert Efendi Pohan Alfa Nelwan Alkhalefi, Dhani Amir, Muh. Ikhsan Aniati Aniati Ardhi, Mega Aulia Ariyanti, Anita Asdinar Asra, Yunelly Asrawati Asrawati, Asrawati AT. Israel, Siti Fitriani Atun, Muslih Awwaliyah, Rahmah Nada El Baci, Muhammad Ihsan Bambang Santosa, Bambang Santosa Bastomi, Mohamad Budi Hartono Budiman, Ismail Custer, Johny Deliana, Marlina Deluma, Ridwan Desi Surlitasari Dewi Dewi Yana, Dewi Dhita, Ferlin Ayp Doni Subrata Eka Wilany Ependi, Rustam Fachrurrezza, Mochammad Fahrul Fahrul Faisal Rahman, Faisal Faizi, M. Nur Faizin, Syuura Annisa Fatma, Ummi Fauziyah, Zakin Fauziyah Gusnarib, Gusnarib Hajar, M. Abduh Ibnu Hamdani Hamdani Handarly, Dolly Hasmira Said, Hasmiras Said Hasniati, Hasniati Hasriani Hasriani, Hasriani HEDDY JULISTIONO Herbowo, Dono Heri Kusmanto, Heri Herlian, Herlian Hermanto Hermanto Hikmatul Amri Hutabarat, Berni Arnita Ibrahim, Siti Fadillah Idward Resha Indiarto Iis Dahlia, Iis Ilham Jaya Ishak Bagea Islawati Isman, Isman Isnaini Isnaini Jumsurizal Jumsurizal Junierissa Marpaung, Junierissa Juwita Boneka Sinaga Kaharuddin Kamaruddin Kamaruddin Kurnia, Muhammad Lianda, Jefri Lilianti, Lilianti Lubis, Maksum Syahri Lukman S. Thahir Lutfi, Furqan Maharani, Sindy Putri Matondang, Emmil Mohd Arief Maulana, Hutomo Atman MAULIDIYAH, NUR Miransyah, Noor Wahyu Mujiati Mujiati Muliyani Muliyani Musbir Musbir Muslimah Muslimah Muthmainah, Resti Siti Nainggolan, Frans Rosaveld Hamonangan Nasaruddin Nasaruddin Nasution, Decy Erni Nasution, Syafriansyah Nurhaya, Nurhaya NURLAELA NURLAELA Nurlaili Nurlaili Nurwahida Alimuddin Nurzaima, Nurzaima Oktaviyani , Tariska Inka Pardomuan, Basrah Pohan, Albert Effendi Prasetyo, Bagas Puliwarna, Tunggul Putra, Hari Rahman, Ahmad Zaky Rahman, Fathul Rahmat Hidayat Rahmi Inayati Rakhmadhan, Iraky Ramadhan, Faridun Ramadhani, Riska Rasul, Mat Reyhan , Muhammad Riana Siahaan Rina Pratiwi, Rina Pratiwi Ririk, Ririk Risman Risman Rudi Salam Sinaga, Rudi Salam Ruslan Ruslan Safnidar Siahaan, Safnidar Safrudin Safrudin Sahil, Muhammad Sahil Rizky Bin Saicindo, Shita Aulia salamah salamah Salnus, Subakir Saragih, Triana Shalehoddin Shalehoddin, Shalehoddin Sidik Sidik Silaban, Royani Singarimbun, Dameka Putra Siti Rahmi, Siti Sitti Nadirah Situmorang, Paulus R Suhardiman Suhardiman Sulastri Manurung, Sulastri Sunandar, Ikbal Sungkono Sungkono Syamsuariadi, Syamsuariadi Syifa Mustika Tarigan, Tariolan Tawil, Muh. Fadhli Tuti Herawati Warjio Warjio, Warjio Yunita Br Sitepu, Sri Yurial Arief Lubis, Yurial Arief Zainal Abidin Zulmita, Zulmita