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Chemical Profiles and Biological Activities of Microalga Chlorella sp. from Southeast Sulawesi Haslianti, Haslianti; Asnani, Asnani; Fristiohady, Adryan; Sadarun, Baru; Sutariati, Gusti Ayu Kade; Sundowo, Andini; Yodha, Agung Wibawa Mahatva; Baharum, Syarul Nataqain; Idin, Sahidin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 2 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.2.233-240

Abstract

Microalgae Chlorella sp. grows in the seas of South-East Sulawesi, Indonesia. However, information on the chemical and pharmaceutical aspects of this species from this region is still limited. Therefore, this research aims to explore the chemical contents and biological activities of Chlorella sp. The sample was collected from the Kali Biru and Nambo waters, Kendari. The microalgae were isolated by the agar plating technique and extracted by ethanol. Chemical content was analyzed by phytochemical screening, Liquid Chromatography-tandem Mass Spectroscopy (LC-MS/MS), Total Phenolics Content (TPC) and Total Flavonoids Contents (TFC). Biological activities evaluation includes antioxidant, toxicity, cytotoxicity and antimicrobial. Antioxidant potency was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and ABTS (2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid). Toxicity and cytotoxicity properties were analyzed by BSLT (Brine Shrimp Lethality Test) and MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays against Breast cancer (MCF-7) cell lines. Antimicrobial potency was tested towards E. coli, S. aureus and C. albicans. The result showed that ethanol extract of Chlorella sp. (EEC) contained phenolics, flavonoids, alkaloids and terpenoids/steroids which were supported by LC-MS/MS data, TPC and TFC with value 150.33 and 33.18 mgGAE.g-1 extract. Seven of ten identified major compounds caused this extract to have antioxidant and anticancer including xanthin, 1,1-Diethyl-3-oxo-2- triazanolate, Azelaic acid, ruspolinone, 6-keto prostaglandin F1a and glycol stearate. In general, the biological activity of EEC is less active than the positive controls for each assay. However, the EEC is more potential to be developed as antioxidant such as sunscreen and anticancer especially breast cancer with IC50 value 151-200 ppm and 100-500 ppm, respectively. 
PENGARUH PROPORSI TEPUNG TEMPE, TEPUNG SAGU DAN DAGING UDANG TERHADAP NILAI ORGANOLEPTIK GIZI NUGGET Lakawa, Stefiana Yunisa; Ansharullah, Ansharullah; Asnani, Asnani
Jurnal Sains dan Teknologi Pangan Vol 9, No 2 (2024):
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jstp.v9i2.47850

Abstract

Penelitian ini bertujuan untuk mengetahui proporsi tepung tempe, tepung sagu dan daging udang terhadap nilai organoleptik dan nilai gizi dalam pembuatan produk nugget. Rancangan yang digunakan yaitu Rancangan Acak Lengkap (RAL) terdiri dari 4 perlakuan daging udang, tepung terigu dan tepung tapioka yaitu P0 (150 g : 75 g : 75 g ), P1 (210 g : 45 g : 45 g), P2 (180 g : 60 g : 60 g) dan P3 (150 g : 75 g : 75 g). Data analisis menggunakan sidik ragam (Analysis of Variance), jika berpengaruh nyata maka dilanjutkan dengan uji Duncan’s Multiple Range Test (DMRT) pada taraf kepercayaan 95% (α=0,05). Hasil penelitian menunjukkan bahwa panelis menyukai produk nugget P1 dengan nilai organoleptik deskriptif warna 3,27 (kuning keemasan), aroma 2,87 (agak beraroma udang), tekstur 3,77 (lembut), rasa 3,13 (agak terasa tempe), organoleptik hedonik warna 4,23 (suka), aroma 3,63 (suka), tekstur 3,87 (suka), rasa 3,97 (suka). kadar air 41,77%, kadar abu 2,05%, kadar protein 12,27%, kadar lemak 13,90% dan kadar karbohidrat 30,00%. Proporsi tepung tempe, tepung sagu dan daging udang beda nyata terhadap analisis kadar air, kadar abu, kadar protein, kadar lemak dan kadar karbohidrat dan memenuhi syarat SNI 6683-2014 terhadap kadar air, kadar protein, kadar lemak dan kadar karbohidrat.
Misperception of the benefits and working mechanism of the National Health Protection in the District of Sumba Timur, Indonesia Asnani; Windi, Yohanes Kambaru; Harnani, Baiq Dewi
International Journal of Public Health Excellence (IJPHE) Vol. 4 No. 1 (2024): June-December
Publisher : PT Inovasi Pratama Internasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55299/ijphe.v4i1.838

Abstract

The National Health Insurance (NHI) of Indonesia covers the health care of nearly the whole population of Indonesia. However, misunderstandings of the benefits and the healthcare access mechanism are still evident. The study aims to identify misperceptions regarding the benefits and working mechanisms of the NHI in Waingapu, Sumba Timur District, Indonesia. A qualitative inquiry identifies the misperception about NHI's benefits and working mechanism. Thirty residents were recruited using the consecutive procedure to participate in the study. The data collections are semi-structured interviews, casual conversations, and inductively analyzed. The qualitative analysis identifies that people perceive that the administrative procedure of health care under NHI is complicated, has unlimited drugs and health care coverage, and the premium contribution is claimable. People perceive the management of NHI to treat them as debtors when they delay paying their contributions. People feel they are being treated unfairly because NHI does not provide all health services, but they ask for a guarantee if NHI does not cover health services. It is concluded that the people are underinformed about NHI's benefits and working mechanism. The management of NHI and associated stakeholders should implement ongoing educational programs and promotional efforts to increase awareness and understanding of the NHI system.
Karakteristik Gelatin Sebagai Alternatif Sumber Gelatin Halal Dari Teripang Pasir (Holothuria scabra) Husnawati; Asnani, Asnani; Wahyuni, Sri; Asjun, Asjun
JSIPi (JURNAL SAINS DAN INOVASI PERIKANAN) (JOURNAL OF FISHERY SCIENCE AND INNOVATION) Vol 8 No 2 (2024): JURNAL SAINS dan INOVASI PERIKANAN
Publisher : Pascasarjana Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jsipi.v8i2.488

Abstract

Tujuan penelitian ini yaitu untuk mengetahui komposisi proksimat, profil asam amino teripang pasir, rendemen dan karakteristik gelatin halal dari teripang pasir yang dihasilkan menggunakan HCl 5% dan asam asetat 5% dengan waktu perendaman yang berbeda. Karakteristik gelatin halal yang dievaluasi meliputi kadar air, abu, protein, pH, viskositas, kekuatan gel, dan gugus fungsi. Jenis penelitian ini yaitu eksperimen laboratorium dengan jumlah sampel penelitian rendemen sebanyak 18 unit perlakuan. Pengujian perlakuan terpilih gelatin yaitu kadar air, abu, protein, pH, viskositas, dan kekuatan gel masing-masing sebanyak 6, sedangkan gugus fungsi sebanyak 2. Hasil pengujian gelatin terpilih yaitu nilai proksimat gelatin yaitu kadar abu,protein, pH, kekuatan gel perlakuan asam asetat dengan lama perendaman 12 jam berbeda nyata dengan perlakuan asam klorida dengan lama perendaman 24 jam. Pada parameter kadar air dan viskositas tidak berbeda nyata. Sedangkan puncak serapan yang teridentifikasi pada perlakuan asam asetat 5% lama perendaman 12 jam yaitu pada panjang gelombang 1236,41 cm-1, 1423,51 cm-1, 1654,98 cm-1, 1701,27 cm-1 dan 3493,20 cm-1. Sedangkan pada perlakuan asam klorida lama perendaman 24 jam yaitu pada panjang gelombang 1236,41 cm-1, 1429,30 cm-1, 1653,05 cm-1, 1701,27 cm-1 dan 3510,56 cm-1.
Penerapan PMR Berbantuan Alat Peraga Obibul Terhadap Hasil Belajar Siswa Pada Materi Operasi Hitung Usman, Kartin; Takaendengan, Bertu Rianto; Asnani, Asnani
Jambura Journal of Mathematics Education Vol 5, No 1: Maret 2024
Publisher : Department of Mathematics, Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jmathedu.v5i1.24365

Abstract

The aim of this research is to determine the differences in student learning outcomes taught using the realistic mathematics approach (PMR) assisted by Obibul teaching aids and the learning outcomes of students taught using the direct learning model. The research method used is the experimental method, with a design namely pretest-posttest control group design. Sampling used a random sampling technique from 3 classes, namely classes VII.A, VII.B and VII.C, two classes were selected as samples with a total of 25 students as respondents for each class. Based on the results of the lottery drawing, class VII.B was selected as the experimental class which used a realistic mathematics approach (PMR) assisted by Obibul teaching aids while class VII.A was selected as the control class which used a direct learning model. The instrument used for research is in the form of a description/essay. Hypothesis testing uses the ANCOVA test which produces Fcount = 5.73, which is compared with the value of 𝐹table = 3.94 so that 𝐻0 is rejected. The results of this research concluded that there were differences in the learning outcomes of students who were taught using the realistic mathematics approach (PMR) assisted by Obibul teaching aids and the learning outcomes of students who were taught using the direct learning model.
TOPONIMI DESA DIKECAMATAN TILONGKABILA BERDASARKAN FAKTOR GEOGRAFI Eraku, Sunarty Sully; Asnani, Asnani; Koem, Syahrizal
Jurnal Swarnabhumi : Jurnal Geografi dan Pembelajaran Geografi Vol. 8 No. 2 (2023): Jurnal Swarnabhumi : Jurnal Geografi dan Pembelajaran Geografi
Publisher : Geography Education Study Program, Faculty of Teacher Training and Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/swarnabhumi.v8i2.8184

Abstract

Tilongkabila is one of the districts in Bone Bolango regency. which consists of 14 villages. Every village possesses different toponyms and historical backgrounds, i.e., Berlian, Bongohulawa, Bongoime, Bonggopini, Butu, Iloheluma, Lonuo, Motilango, Moutong, Permata, Tamboo, Toto Utara, Tungulo, and south Tungulo village.  This research aimed to study toponym geography in TilongkabilaDistrict.It employed a qualitative descriptive method and spatial approach analysis. The data was collected through observation, interviews, documentation, and library research. The result showed two factors related to the basis of village toponymy in Tilongkabiladistrict. First, non-physical geographic factors (social and cultural aspects)were measured by 78%. Further, physical factors such as the embodiment aspect are influenced by 27%. All in all, the study concluded that non-physical geographic factors influenced the village toponymy in TilongkabilaDistrict.
Model niat berperilaku melibatkan citra merk dan kepercayaan dalam niat pembelian produk fashion secara online pada Generasi Milenial di Kota Batam Asnani, Asnani; Riki, Riki
Bahtera Inovasi Vol 8 No 1 (2024): Jurnal Bahtera Inovasi
Publisher : Program Studi Manajemen FEBM UMRAH

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31629/bi.v8i1.7032

Abstract

This study aims to analyze the role of brand image on purchase intention mediated by trust. This research uses quantitative research methods. The population used in this study is the millennial generation who have purchased fashion products online in Batam City. The technique used in sampling this research is purposive sampling, namely the selection of samples selected based on consideration of convenience sampling techniques, where the sampling technique by sampling by chance which is considered as a sample member is people who are easy to meet, are at the right time, and are easy to reach. The criteria are the millennial generation who have purchased fashion products through e-commerce platforms in Batam who were born around 1980 to 1995 when technology was advanced. Data analysis used descriptive analysis, outer model analysis, inner model, and mediating hypothesis testing through the SmartPLS programme. The results showed that brand image has a significant effect on trust, trust has a significant effect on purchase intention, while brand image has no significant effect on purchase intention, but trust is able to mediate between brand image and purchase intention
Contributory Factors to the Inability of the Poor to Access National Health Protection: A Case Study in Waingapu, Sumba Timur District, Indonesia Windi, Yohanes Kambaru; Harnani, Baiq Dewi; Asnani
Journal of Community Medicine and Public Health Research Vol. 5 No. 2 (2024): Journal Community Medicine and Public Health Research
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jcmphr.v5i2.57065

Abstract

Millions of poor Indonesians still need access to Indonesia's National Health Protection (NHP). The study investigated barriers making NHP inaccessible for the poor in Waingapu, Sumba Timur, Indonesia.  We used a qualitative approach to identify the contributing factors to why NHP was inaccessible for some populations. A total of 30 residents of Waingapu who attended healthcare in two hospitals and five Community Health Centers were recruited using convenience technic. Twenty residents participated in the semi-structured interview, while ten engaged in casual conversations. We also interviewed three heads of community groups and three health cadres for comparative information. Questions have been developed to guide the interviews and casual conversation. The interviews were audio-taped and transcribed while intensive note-taking was performed during the informal discussion or the conversation was narrated immediately after the talks ended. The information was inductive, analyzed, and coded to produce common information among the participants. The data analysis revealed the factors that contributed to the failure of the resident to enroll in the NHP, such as lack of information about the registration, poor socialization of the program, the ignorance of village officers, unaffordability, and confusion about the parties responsible for the registration. The study concluded that poor people were still vulnerable to access free coverage and healthcare from the NHP. The stakeholders of the NHP, especially the local management of NHP and local government, need to urgently and effectively educate the commoners about the mechanism, benefits, and contribution to the NHP to ensure equity and equality in accessing quality healthcare.
Bimbingan Teknis Produk Olahan Ikan Menjadi Cemilan Sehat dan Kekinian Sebagai Peluang Wirausaha serta Untuk Mencegah Stunting Bagi Anak Nelayan di Desa Wawobungi Kabupaten Konawe Asnani; Andi Besse Patadjai; Azis, Nurdiana A; Wa Ode Piliana; Hasnia Arami; Wellem H Muskita; Baru Sadarun
Jurnal Pengabdian Meambo Vol. 2 No. 2 (2023): Jurnal Pengabdian Kepada Masyarakat MEAMBO
Publisher : PROMISE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56742/jpm.v2i2.66

Abstract

Pengabdian ini dilakukan berdasarkan data survei bahwa sebagian besar para wanita nelayan/istri nelayan di Desa Wawobungi hanya melakukan aktivitas domestik dalam rumah tangga saja, tanpa melakukan kegiatan yang bisa membantu meningkatkan perekonomian keluarga. Selain itu, sebagian besar ibu rumah tangga juga belum mendapatkan informasi dan pengetahuan tentang bagaimana cara mengolah sumberdaya ikan menjadi panganan yang bergizi tinggi untuk mencegah stunting anak nelayan serta berpeluang untuk dikomersialkan dilingkungan desa maupun di swalayan di Kota Kendari. Hal tersebut menjadi permasalahan khususnya mitra sasaran, diantaranya rendahnya atau kurangnya informasi dan pengetahuan oleh mitra sasaran dan keterampilan dalam pengolahan hasil perikanan yang bernilai gizi tinggi untuk mencegah stunting bagi anak nelayan, kemudian guna menumbuhkan jiwa wirausaha dan peningkatan kreatifitas wanita nelayan serta meningkatkan penghasilan keluarga. Hasil pengabdian menperlihatkan respon dan partisipasi sangat baik dari mitra sasaran, hal tersebut dibuktikan dengan tingginya antusias dari peserta bimtek. Sehingga kedepannya perlu pendampingan lanjutan dalam hal pendampingan perizinan usaha dan kemasan produk serta manajemen usaha yang berkaitan dengan pembukuan usaha keluarga, sehingga diharapkan kesejahteraan masyarakat pesisir dapat meningkat serta menciptakan generasi anak nelayan yang sehat dan cerdas
Literasi Blockchain untuk Industri Halal dan Pendampingan Investasi Keuangan Digital Kadir, Syahruddin; Helty; Asnani; Rosmaeni
Edusight Jurnal Pengabdian Masyarakat Vol. 2 No. 1 (2025): Edusight Jurnal Pengabdian Masyarakat
Publisher : Yayasan Meira Visi Persada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69726/edujpm.v2i1.72

Abstract

Literasi adalah kegiatan sosialisasi dan pembelajaran tentang blockchain sebagai teknologi masa depan yang dilakukan oleh Prodi Manajemen Universitas Sipatokkong Mambo kepada masyarakat umum dan civitas akademi. Dalam kegiatan memberikan literasi, Prodi Manajemen Universitas Sipatokkong Mambo bekerjasama dengan Rumah EKIS (Forum Mahasiswa Ekonomi Islam). Tujuan penulisan ini yang pertama adalah untuk membahas bagaimanakah literasi blockchain yang diberikan kepada masyarakat di kabupaten Bone. Tujuan yang kedua adalah menjelaskan sistem blockchain, dan peluang penggunaannya pada industri halal. Metode penelitian yang dipakai pada penelitian ini menggunakan ceramah dan pendampingan dengan sumber data dari studi literatur artikel jurnal, beberapa situs terkait, platform dan aplikasi. Hasil dari kegiatan ini menunjukkan bahwa pentingnya literasi tentang teknologi blockchain untuk industri halal dan potensinya sebagai teknologi transformasi di masa depan. Selain itu, masyarakat dan mahasiswa ekonomi Islam di Kabupaten Bone tergolong ke dalam less literate dan less of trust karena sebagian masyarakat dan mahasiswa ekonomi Islam di Kabupaten Bone hanya mengerti terhadap blockchian yang digunakan untuk Bitcoin atau decentralized finance dan hanya mempertimbangkan aspek keutamaan fasilitas layanan keuangan kripto tersebut. Dengan terlaksananya kegiatan ini mampu meningkatkan pemahaman yang lebih baik terkait penggunaan blockchain pada industri halal, dan masyarakat melek digital untuk melakukan investasi aset kripto. Implikasi kegiatan ini diharapkan membuka ruang diskusi selanjutnya untuk pembahasan mendalam tentang potensi dan peluang pemanfaatan teknologi blockchain pada sektor-sektor lain.
Co-Authors Abd. Rasyid Syamsuri Abigael Kabe Agung Nugraha Ahmad Mustafa Ahsoni, Aziz Amin Hidayat Anak Agung Istri Sri Wiadnyani Anastasia Wiwik Swastiwi Andi Besse Patadjai Andi Zakiah Aini Maftuha Andini Sundowo Angelia Sabina Sinaga Ansharullah ansharullah Arami, Hasnia Arby'in Pratiwi Arini, Jenny Arzul Rahmat Asdar Asis, Zalsabila Reskyta Asjun Asjun, Asjun Astri Rahayu Azis, Nurdiana A Baharum, Syarul Nataqain Baiq Dewi Harnani R Balubi, Abdul Muis Bartoven Vivit Nurdin Baru Sadarun Baru Sadarun Battu, Sutrisno Prasetyo Bertu Rianto Takaendengan Betty Sri Laksmi Jenie Bunga Lestari Chairunnisa, Dita Daniningsih, Tri Date, Yana Meutia Yunus Devi Pratiwy Diki, Muhammad Ihsan Dina Dina, Dina Dwi Budi Santoso Dzulzahabiyyah, Afifah Azhari Effendi, Wa Ode Nilda A. Eraku, Sunarty Sully Faisal Faisal Danu Tuheteru Fatimah Yunus, Andi Fatimah, Shalawatun Amaliah Fatmaliza Harahap Febrianto, Fandi Fera, Feti Firda Julia Fristiohady, Adryan Gusti Ayu Kade Sutariati Hadjar, Nurhayati Hamzah, Nurnaningsih Haslianti Haslianti, Haslianti Hasnia Arami Helty Hestiani, Hestiani Hidayati Hidayati Husna Husna Husna Husnawati I Nyoman Sujana Ibrahim, Moh Nuh Ibrahim, Mohammad Nuh Idin, Sahidin Ima Wijayanti Indayani, Mimin Kaidah Indriani Indriani Iralistari, Waode Iralistar Irdam Riani, Irdam Isamu, Kobajashi Togo Israwati Jali, Wa Jaya, Makmur Johan, Arniati Juliarto, Dion Kadir, Muh. Ridho Syafarullah Kadir, Syahruddin Kaneko, Masanori Karamoi Karamoi Karimuna, Siti Rabbani Karmin Karmin, Karmin Kartin Usman Kartini Kartini Kasim, Ma'ruf Kasmiati Kristi, Elsa La Ode Alirman Afu, La Ode Alirman La Ode Baytul Abidin La Sara Lakawa, Stefiana Yunisa Laksanany, Satya Agustina Lolopayung, Selin M. Manugeren, M. Ma’ruf, Khaidhar Maftuha, Andi Zakiah Aini Mardiansyah Mardiansyah, Mardiansyah Maulana, Fazirul Maulana, Mey Sri Mekuo, Febrianto Nur Miftahul Hasanah Mila Angraeni Mita Gebriella Inthe Mohd Fauzi, Mohd Muh Aksa Azis Muh. Fitra Annaba Muhaimin Hamzah Muhammad Fajar Purnama Muhammad Trial Fiar Erawan Muhtar, Nur Inayah Muskita, Wellem Hendrik Nancy D. Yuliana Nanda Visca Pradiny Naslina Alimina Nasution, Ali Napiah Niaga, Muh. Syawal Akbar Novi Damayanti Nur Asyik, Nur Nur, Indriyani Nuraeni, Febrianti Nurfitri Ekantari Nurhawa Oce Astuti Pairulsyah Pairulsyah Pardi Pardi Pardi Pardi, Pardi Patadjai, Andi Besse Pertiwi, Gadis Andista Piliana, Wa Ode Pratiwy, Devi Putri, Rizki Amelia Eka Rahmah, Alfiyyah Nur Ramadhan, Muhammad Rahmad Raup, Syahril Abd. Resi, Resi RH. Fitri Faradilla Ridhowati, Sherly Riki RIKI RIKI, RIKI Rizky Ayu Lestari Rosmaeni Rosnawintang Rosnawintang Rusmianur, Wa Ode Sadimantara, Muhammad Syukri Saediman, Haji Safitra, Safitra Safitri Hariani Safitri, Lia Safitri, Wa Ode Mustika Ayu Safitri Sahlan Sahlan Saleh, Arum Ayu Gustiara Absyah Sali, Fitriani Salsabila, Febi Nur Sari, Ifaty Fadliliana Sari, Wilda Purnama Sarinah Sarinah Setiawati Anggraini Siti Rabbani Karimuna SITTI HAJAR Sri Rejeki Sri Wahyuni Sri Wahyuni Sri Wulan, Sri Suadi Suadi Sukma, Asca Rahayu Suriani Suriani Surni, Surni Suwarjoyowirayatno, Suwarjoyowirayatno Syahrizal Koem T. Thyrhaya Zein Tamrin Tamrin Tanjung, Dinda Andini Thesalonica, Denysha Turki, Syifaa Sabianova Addina Vatma, Ona Sri Wa Ratmi Wellem H Muskita Wellem H. Muskita Winandari, Alzagita Trisazena Windi, Yohanes Kambaru Winiati P. Rahayu Wiwin Rahmawati Nurdin Yodha, Agung Wibawa Mahatva Yundari, Yundari Yuni Ratnasari Yusnaini Yusnaini Zainun, Mariana