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All Journal ACTA VETERINARIA INDONESIANA Jurnal Pendidikan Vokasi Jurnal Veteriner Jurnal Ilmu Ternak International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Farmasi Klinik Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Edik Informatika : Penelitian Bidang Komputer Sains dan Pendidikan Informatika Jurnal Bisnis, Manajemen, dan Informatika JETT (Jurnal Elektro dan Telekomunikasi Terapan) JURNAL ILMIAH PETERNAKAN TERPADU Creative Research Journal ARSHI Veterinary Letters J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan ZIRAA'AH MAJALAH ILMIAH PERTANIAN At-Tajdid : Jurnal Pendidikan dan Pemikiran Islam Media Kontak Tani Ternak Jurnal Farmasi dan Sains Indonesia (JFSI) Journal of Science and Education (JSE) Jurnal PTI (Jurnal Pendidikan Teknologi Informasi) PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Masyarakat Bestari (JPMB) Arcitech: Journal of Computer Science and Artificial Intelligence Journal of Applied Business and Technology Jurnal Kedokteran Hewan Farmers: Journal of Community Services The Indonesian Journal of Computer Science Jurnal Agripet ORAHUA : Jurnal Pengabdian Kepada Masyarakat Journal of Social and Community Development Journal of Medicine and Health

Rini Widyastuti

Laboratorium Reproduksi Dan Inseminasi Buatan, Departemen Produksi Ternak, Fakultas Peternakan, Universitas Padjadjaran

Author-ID : 2699294

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Acute Iron Dextran Injection Increases Liver Weight and Reduces Glycerol Kinase Expression in Liver Ramdan Panigoro; Fadhal M. Ahmad; Uni Gamayani; Neni Anggraeni; Rini Widyastuti; Mohammad Ghozali; Mas Rizky A.A Syamsunarno
Indonesian Journal of Clinical Pharmacy Vol 7, No 4 (2018)
Publisher : Universitas Padjadjaran

Iron is essential and needed in a very small amount. When iron exceeds normal need, metabolic alteration occurs, causing hepatosteatosis. The mechanism of iron inducing hepatosteatosis remains unclear. Glycerol kinase, the enzyme responsible in triglyceride synthesis initiation, is assumed to have a role in the pathomechanism of hepatosteatosis. This study aimed to investigate the gene expression of glycerol kinase in an acute iron overload condition. This study was conducted in Animal Laboratory Faculty of Medicine and Central Laboratory Universitas Padjadjaran from May to June 2017. Three groups of mice were divided by the dose of iron dextran injection (0, 0.1, 0.3 mg/day/mice). After 19 days, mice were terminated, liver weight was measured and glycerol kinase gene expression in the liver was determined by semi-qualitative PCR. Quantification of PCR result was calculated by ImageJ software. There was a significant change in liver weight of the mice in a dose-dependent manner of iron injection. The expression of glycerol kinase tended to decrease, but statistically insignificant. Acute iron dextran injection increases liver weight and tends to reduce glycerol kinase gene expression in mice liver.Keywords: Glycerol kinase, hepatosteatosis, iron overload Efek Zat Besi Dosis Tinggi Akut dalam Meningkatkan Berat Organ dan Menurunkan Ekspresi Gliserol Kinase HeparAbstrakZat besi merupakan nutrien esensial dan diperlukan dalam jumlah yang sangat kecil. Ketika kadar zat besi melebihi kadar normal dalam tubuh, terjadi perubahan metabolisme yang menyebabkan hepatosteatosis. Mekanisme zat besi dalam menyebabkan hepatosteatosis masih belum diketahui secara pasti. Gliserol kinase, enzim yang menginisiasi sintesis trigliserida, diduga berperan dalam patomekanisme hepatosteatosis. Penelitian ini bertujuan untuk meneliti ekspresi gen gliserol kinase pada hepar pada kondisi tinggi zat besi akut. Penelitian ini dilakukan di Laboratorium Hewan Fakultas Kedokteran dan Laboratorium Sentral Universitas Padjadjaran dari bulan Mei sampai dengan Juni 2017. Tiga kelompok mencit dibagi berdasarkan dosis injeksi iron dextran intraperitoneal (0, 0,1, 0,3 mg/hari/ekor). Setelah 19 hari, mencit diterminasi, berat hepar ditimbang dan ekspresi gen gliserol kinase diukur dengan metode semi-kualitatif PCR. Kuantifikasi hasil PCR dilakukan dengan menggunakan aplikasi ImageJ. Terdapat peningkatan berat hepar secara signifikan yang sejalan dengan dosis ijeksi zat besi. Ekspresi gen gliserol kinase cenderung menurun, meskipun secara statistik tidak signifikan. Keadaan tinggi kadar zat besi yang akut meningkatkan berat hepar dan cenderung menurunkan ekspresi gen gliserol kinase pada hepar mencit.Kata kunci: Gliserol kinase, hepatosteatosis, zat besi berlebih

Tampilan Kesuburan Sapi Bali Induk yang Dikawinkan Langsung dengan Pejantan dan Inseminasi Buatan Ketika Estrus Hasil Sinkronisasi Menggunakan PGF2α Petrus Kune; Rini Widyastuti; Takdir Saili
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 2 (2019): JITRO, Mei
Publisher : Universitas Halu Oleo

ABSTRAKAngka kebuntingan sapi bali yang mengalami sinkronisasi estrus dan diinseminasi pada saat estrus muncul masih di bawah 50%. Akan tetapi, persentrasi gejala estrus yang muncul setelah sinkronisasi selalu di atas 85%. Oleh karena itu, penelitian untuk mengevaluasi tingkat kebuntingan sapi bali setelah sinkronisasi, deteksi estrus dan perkawinan baik kawin alam maupun inseminasi telah dilakukan dengan menggunakan 67 ekor sapi bali induk umur 4-6 tahun yang mempunyai corpus luteum periodik. Penelitian dilakukan sebanyak empat tahap dengan masing-masing tahap selama 22 hari. Semua sapi percobaan dipelihara secara intensif pada 5 pedok yang terpisah dan setiap pedok ditempatkan 4 ekor sapi bali induk dan seekor pejantan. Hormon estron digunakan untuk sinkronisasi induk sapi percobaan dengan dosis 5 ml/ekor secara intra muscular, selanjutnya dilakukan induk sapi dikawinkan pada hari pertama sampai kelima setelah sinkronisasi dan deteksi estrus. Deteksi estrus diperpanjang sampai dengan akhir siklus estrus. Hasil penelitian menunjukkan bahwa 91,05% sapi percobaan mengalami estrus setelah sinkronisasi sedangkan gejala estrus muncul mulai hari pertama sampai hari kelima (rata-rata hari ke 3) setelah sinkronisasi. Angka kebuntingan yang diperoleh rata-rata 27,87%. Estrus alami (terjadi setelah estrus sinkronisasi) mulai muncul setelah 6-13 hari (rata-rata 8 hari) estrus hasil sinkronisasi muncul.Kata Kunci: estrus, kebuntingan, PGF2α Therefore, the experiment related to evaluation of conception rate of bali cow following synchronization, estrus detection and natural mating or artificial insemination was conducted using 67 bali cow aged 4-6 y.o. that has periodic corpus luteum. The experiment was conducted in four sequent periods (22 days each). All animal was kept intensively in 5 separate paddocks where each paddock was allocated five animals (4 cows+1 bull). Estron contained PGF2α was injected intramuscular (5 ml/head) to all cows for synchronization while mating (natural mating and artificial insemination) was conducted during 1-5 days following synchronization and estrus detection. Estrus detection was prolonged up to the end of estrus cycle. The results showed that 91.05% of synchronized cow was estrus, while the estrus sign occurred on day 1-5 (day 3 in average) after synchronization. Conception rate gained in this experiment was 27.87%. Natural estrus that occurred following synchronized estrus varied between 6-13 days (8 days in average) after synchronized estrus occurred.Keywords: conception, estrus, PGF2α, synchronization

Anemia and Eosinophilia in Traditional Goat Farmers: Early Markers of Strongyle Zoonoses (ANEMIA DAN EOSINOFILIA PADA PETERNAK KAMBING TRADISIONAL: PENANDA AWAL ZOONOSIS STRONGYLUS) Insy Nafisah Taufik; Henhen Heryaman; Rini Widyastuti; Mas Rizky A. A. Syamsunarno; Mohammad Ghozali
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The spread of zoonoses between humans and animals can increase with the presence of specific contacts between the two. As well as intensive contact between farmer and goat. Anemia is a common hematologic change encountered in infection and zoonoses so it can be used as an early indicator for zoonoses. Nevertheless, anemia itself cannot lead to a specific etiology thus differential leukocyte count and goat fecal examination can be a good supporting test. Understanding the etiologies of the disease is critically important in preventing the decline in human’s quality of life as well as economic losses. This cross-sectional study included 30 farmers who were recruited by purposive sampling method. They agreed to do a CBC examination with 22 parameters. From the test, 37% of them showed mild anemia. There were 45% farmers with microcytic hypochromic anemia while the rest had normocytic normochromic anemia. There was an increased number in eosinophil and segmented neutrophil from anemic farmers, 18% and 9% respectively. Besides, 13% of goats physical examination indicated as below normal conditions characterized by lean bodies, lymph node swelling, and pale mucosa. Whereas 38% goat’s fecal test showed evidence of Strongyle eggs. The research suggests a possible interrelation between animals, humans, and the environment with an increased risk of zoonoses. Anemia and eosinophilia that occur can be an early marker which will be decisive not only for the success of the disease progression reduction but also the long term suppression of the economic loss.

Low Concentration of Ethylene Glycol Improved Recovery Rate of Human Spermatozoa After Vitrification (ETILEN GLIKOL KONSENTRASI RENDAH MENINGKATKAN RECOVERY RATE SPERMATOZOA MANUSIA PASCAVITRIFIKASI) Rini Widyastuti; Sony Heru Sumarsono; Arief Boediono; Siti Darodjah Rasad
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The use of cryoprotectants for the cryopreservation of human spermatozoa, oocytes, zygote, earlycleavage stage of embryos and blastocyst is an integral part of almost every human In Vitro Fertilizationprogram. Moreover, the cryopreservation of these types of cells by direct plunging into liquid nitrogen (-196°C) usually requires a high concentration of cryoprotectant with a consequent of cytotoxic effect. Theaim of this study was to observe the effect of ethylene glycol concentration on the spermatozoa recoveryrate following vitrification process. Earle’s balanced salt solution + 0.25 M sukrosa + 1 % human albumineserum as basic solution supplemented with some different concentrations of etylene glycol (ie: 36.25%;18.25%; 9.12%; 4.56%; 1.14% and 0.57%) were used to evaluate the motility and viability of spermatozoafollowing vitrification. Human’s spermatozoa from ejaculates with progressive motility and viability above50% were used as samples. Samples were mixed with vitrification solution and then loaded into 0.25 mLstraws, equilibrated for 10 minutes at room temperature before plunged into liquid nitrogen. Spermatozoathawing was done at 24 hours after the vitrification. The results showed that, the decrease of spermatozoamotility and viability were observed at the highest (100%, 96.70%, respectively) in the samples that wereadded with vitrification medium contained 36.25% of ethylene glycol. On the other hand, the decrease ofthe spermatozoa motility and viability were found at the lowest (14.11%, 43.81 %, respectively) in thesamples without ethylene glycol supplementation. It can be concluded that the highest spermatozoa recoveryrate was obtained from the vitrification using a low concentration of ethylene glycol.

Mice Oocytes Respond after Vitrification Followed by Artifical Activation Using a Various Concentration of Strontium Chloride and Cytochalasin B Muhammad Rosyid Ridlo; Rini Widyastuti; Alkaustariyah Lubis; Mokhamad Fahrudin; Arief Boediono
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Oocyte vitrification is the important part of gamete preservation for further purpose. The objective of this study was to evaluate the response or development of vitrified-mice oocyte following activation using various concentrations of Strontium Chloride (Sr Cl2). Oocytes were collected from superovulation-induced female mice. Oocytes vitrification was then performed using a gradual equilibration of 2 M Ethylene Glycol in 0.25 M sucrose and 7 M Ethylene Glycol on 0.5 sucrose. Subsequently, the vitrified oocytes were thawed and activated using various Strontium Chloride concentration in each group. Control 1 is unvitrified oocyte and without Sr Cl2. Control 2 is unvitrified oocyte then activated by 20 mM Sr Cl2. Zero (0) mM Sr Cl2 is vitrified oocyte without Sr Cl2. Group Ten (10) mM is vitrified oocyte then activated by 10 mM Sr Cl2. Group Twenty (20) mM is vitrified oocyte then activated by 20 mM Sr Cl2. The viability of vitrified-thawed oocytes was observed based on ooplasm integrity. Whereas the oocytes respond to artificial activation was observed based on pronucleus formation after 10 hours of activation. The result showed that 39% of oocyte degenerated following vitrification. The respond of vitrified-thawed oocytes following artificial activation using Strontium Chloride was significantly lower compared to fresh oocytes (p<0,05). Interestingly the highest percentage of activated oocytes (36.36%) was present in a group achieved 20 mM Strontium Chloride. As conclusion is Strontium Chloride 20mM has a best result (36,36%) to activate vitrified oocyte than 0 mM and 10 mM of Strontium Chloride.

Derajat Pemulihan dan Persentase Spermatozoa X dan Y Kambing Peranakan Etawah Setelah Separasi dengan Gradient Percoll (RECOVERY RATE AND PERCENTAGE OF SPERMATOZOA X AND Y OF ETAWAH CROSSBREED GOAT AFTER SEXING WITH GRADIENTT PERCOLL) Siti Darodjah Rasad; Rangga Setiawan; Nurcholidah Solihati; Rini Widyastuti; Ilham Nugraha
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Aim of the research is to find out recovery rate and percentage spermatozoa X and Y after sexing with gradientt Percoll in Etawah Crossbreed Goat. Extender was used TRIS Egg Yolk. The semen sample from five male Etawah Crossbreed Goat were used in this research. Gradientt percoll performed 10 fraction layer with centrifugation time 10 minute at 2500 rpm. The data were analyzed descriptively. Result of the research shows that average recovery rate of spermatozoa X is 50.96±26.07 % and spermatozoa Y is 44.13±6.11 %. Average percentage of X sperm is 78.00±7.06 % and Y sperm is 76.10±5.95 % .

In-vivo Mice Pre-Implantation Embryo Development after Oral Administration Ethanolic Extract of Cogon Grass Roots (Imperata cylindrica L) Jaqueline Sudiman; Rini Widyastuti; Madeline Priscilla; Alkaustariyah Lubis; Mas Rizky Anggun Adipurna Syamsunarno; Sony Heru Sumarsono
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Cogon grass (Imperata cylindrica L) is known as a medicinal plant that is scattered almost worldwide. Despite its role that inhibits another plant’s growth, cogon grass possesses several benefits in health. This research has to identify the effect of short-term gavage ethanolic extract of cogon grass roots (CGG) to in-vivo mice preimplantation embryo development. A total of 60 female mice were divided into control and treatment groups, dosages at 90 and 115 mg/kg of body weight of CGG, orally gavage for 20 days. The superovulation of mice was done at the end of the CGG treatment by injecting 5 IU Pregnant Mare Serum Gonadotropin (PMSG) and after 48 hours, followed by 5 IU Human Chorionic Gonadotropin (hCG) injection and directly the mice were mated. The mating rate was checked by the appearance of the vaginal plug 12 hours after hCG injection. Mice were sacrificed, the oviducts and cornua of uteri were isolated to collect the oocytes and embryonic cells by flushing the oviducts and cornua uteri with Phosphate-buffered saline (PBS). The effects of CGG as an antifertility were evaluated by measuring the number of oocytes, fertilization, and in-vitro embryo development rates. The results showed significantly reduced about half of the mating rate in the 115 mg/kg BW group (p<0.05) compared to control. However, the 90 mg/kg BW dose reduced 20% mating rate compared to control, and not significant (p>0.05). In all treatment groups, only half oocytes fertilized. The cleavage and blastocyst rate in 115 mg/kg BW group were significantly lower compared to the control group (p<0.05). In conclusion, oral gavage of cogon grass root ethanolic extract disrupts the mating process and development of in-vivo mice preimplantation embryo development.

Oral Administration of Cogongrass (Imperata cylindrica L) Root Ethanol- Extract causes Mouse Epididymal Sperm Abnormality (PEMBERIAN EKSTRAK ETANOL AKAR ALANG-ALANG (IMPERATA CYLINDRICA L) SECARA ORAL MENYEBABKAN ABNORMALITAS SPERMA EPIDIDYMIS MENCIT) Rini Widyastuti; Jaqueline Sudiman; Tyagita Tyagita; Mas Rizky Adipurna Anggun Syamsunarno; Sony Heru Sumarsono
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Sperm morphology is an important parameter to be observed in the male fertility. Some of the bioactive compounds of cogongrass root such as alkaloid and terpenoid, affect male fertility by interference the spermatogenesis. The objective of the study was to observe the effect of cogongrass root ethanol extract on mouse sperm morphology. This study was carried out by oral administration of two different doses i.e 90 and 115 mg/kg body weight of cogongrass root ethanol extract into 8-10 weeks old DDY strain mice for 14 days to evaluated the acute effect due to the administration of cogongrass root ethanol extract on mouse sperm morphology. The results showed that treatment with cogongrass root ethanol extract significantly increased sperm abnormalities followed a dose depending pattern (p<0.05). Interestingly, the administration of cogongrass root extract did not affect sperm head morphology but tailless, folded and bent sperm increased linearly with the administration dose of cogongrass root ethanol extract. In conclusion, cogongrass root ethanol extract causes secondary sperm abnormalitties on mouse sperm.

The Effect of Ethanol Extract of Cogon Grass Root (Imperata cylindrica) to Estrus Cycle and Metabolite Profile in Female Mice Sondi Robianto; Mas Rizky Adipurna Anngun Syamsunarno; Alkaustariyah Lubis; Madeleine Priscilia; Neni Anggraeni; M. Ghozali; Mulyanusa Ritongga; Rini Widyastuti
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The use of herbs as contraception has become the focus of current contraceptive development.. In the previous study, the extract ethanol of cogon grass roots (Imperata cylindrica) has the effect of decreasing sperm production and changing metabolite profiles in male mice. However, the effect of this Indonesian well-known herb is still indefinite in female.The purpose of this study was to investigate the effect of ethanol extract of cogon grass roots to the fertility of female mice. The study was conducted at Animal Laboratory of Faculty of Medicine and Central laboratory Universitas Padjadjaran from November to December 2017. Mice were given ethanol extract of cogon grass roots 90 and 115 mg/kg body weight per oral. After three weeks, vaginal cytology was observed and metabolite profiles were measured. The results showed a shortening of the estrus phase in 90 and 115 mg/kg treatment group. However, the level of glucose and cholesterol in serum were not different among groups. This study indicated the extract ethanol of cogon grass roots has potential effect as contraceptive agent and the effect is independent from metabolite profiles. Keywords: Cogon Grass, Imperata cylindrica, Estrus Cycle Phase, Metabolite Profile

Pregnancy Rate after Intrauterine Insemination with the Presence or Absence of Leukocytospermia in Sperms Prepared using Density Gradient Method Rini Widyastuti; Julius Pangayoman; Aida Riyanti; Alkaustariyah Lubis; Mas Rizky Anggun Adipurna Syamsunarno
International Journal of Integrated Health Sciences Vol 6, No 2 (2018)
Publisher : Faculty of Medicine Universitas Padjadjaran

Objective: To examine the association between different concentrations of leukocyte and sperm recovery rate after sperms are prepared using density gradient method and pregnancy rate after intrauterine insemination (IUI). Increased leukocytes in semen have been associated with increased reactive oxygen species (ROS) that reduces sperm quality.Methods: Semen samples that were collected from 31 male partners of couples undergoing infertility investigation were analyzed for sperm concentration, motility, and leucocytes concentration. Semen samples were then divided in two groups based on their leucocytes concentrations (category A: >0 to <1 × 106/mL; category B: >1 x 106/mL. Semen samples were processed using density-gradient centrifugation technique. Results: There was a significant difference in the number of sperms harvested and sperm motility after preparation. Interestingly, pregnancy rate after IUI was higher (p<0.05) in non-leukocytospermia semen (39%) when compared to leukocytospermia semen (30%).Conclusions: Seminal leukocytes (PMNL) concentration affects pregnancy rate after intrauterine insemination. Keywords: Density gradient method, sperm recovery rate, intrauterine insemination, pregnancy rate DOI: 10.15850/ijihs.v6n2.1318

Co-Authors Abdullah, MT, Dr. Rijal Ade Fitri Rahmadani Ade Irma Suryani Ade Riki Kartana Aida Riyanti Alam, Sri Rahayu Alda Prayoga, Rama Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alvin Yusuf An An Nurmeidiansyah, An An An An Yulianti Andi Hiroyuki Anggareni, Neni Annisa Yusrina Apriana, Diwi Arief Boediono Asarina, Shinta Ashabul Khairi Asmar Yulastri Asri Wulansari, Asri Bayani, Matni Syifa Dahlia Setiawan Dani Garnida Daud, Andre Rivianda Deru Indika Deru R Indika Desi Yunita Desti Ranihusna Diah Nugrahani Pristihadi Diding Latipudin Diky Ramdani dudi, dudi Dumono DWI ANDI NURMANTRIS Dwi Cipto Budinuryanto Dwi Wahyudha Wira Edianingsih, Primiani Elizamiharti Emini, Emini Fadhal M. Ahmad Fadjeri, Indrayati Fahmy Avicenna Faradana, Najma Rika Farel Fairuz Fauziah, Nisa Fauziah, Nisa Fauziati, Popi Firmansyah, Indra Fitri Rahmadani, Ade Ganefri Gea, Yufen Yuliandri Gita Widya Pradini Habibullah, Hafizh Henhen Heryaman Heryawan Kemal Mustafa Husna, Afifah Iin Susilawati Ilham Nugraha Iman Hernaman Inna Kholidasari Insy Nafisah Taufik Ismiraj, Muhammad Rifqi Jaqueline Sudiman Julius Pangayoman Karisma Mardatillah Karmila Suryani Karmila Suryani Kaseri, Kaseri Kasihani, Ni Nyoman Khairudin Khairudin Khoirinaya, Candrani Khotimah, Devi Syahrilia Kikin Winangun Kikin Winangun Krismadinata Krismadinata, Krismadinata Kurnia A Kamil Lubis, Alkaustariyah M. Dyon M. Ghozali M. Rosyid Ridlo Madeleine Priscilia Madeline Priscilla Mansyur . Mas Rizky A. A. Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A. Syamsunarno Mas Rizky Adipurna Anngun Syamsunarno Mohamad Ghozali Mohammad Ghozali Mohammad Ghozali, Mohammad Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muhammad Rifqi Ismiraj Muhammad Rosyid Ridhlo Muhammad Rosyid Ridlo Mulyanusa Ritongga Murlena, Murlena Mushawir, Andi Nahak, Martinus Nelfira Nena Hilmia Neni Anggraeni Neni Anggraeni Nizwardi Jalinus Novi Mayasari Novi Mayasari Novrita, Sri Zulfia Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurfitri Handayani, Nurfitri Nurul Ikhwan Pangestu, Muhammad Syahid Panji Petrus Kune Pisriwati, Siti Atwano Pradini, Gita W Priscilla, Madeleine Radial Anwar, Radial Rahmadani, Ade Fitri Rahmat Hidayat Ramdan Panigoro Rangga Setiawan Rangga Setiawan Refdinal, Refdinal Ridhlo, Muhammad Rosyid Ridwan Riska Amelia Rizka Amelia Rizki, Yovan Robianto, Sondi Romi Zamhir Ronnie Permana SATRIYAS ILYAS Sefriani, Rini Sigit Prastowo Sigit PRASTOWO Sigit Prastowo Siswanto, Deny Hadi Siti Darodjah Rasad Siti Darodjah Rasad Siti Darodjah Rasad Siti Darodjah Rasad Soeparna Soeparna Soeparna Soeparna, Soeparna Sondi Robianto Sony Heru Sumarsono SONY HERU SUMARSONO Sony Heru Sumarsono SONY HERU SUMARSONO Sony Heru Sumarsono Sri Restu Ningsih Sri Suwarni Sudiman, Jaqueline Suhaida, Jihan Sukamtono Sukamtono Syamsunarno, Mas Rizky A. A Syamsunarno, Mas Rizky A. A. Syamsunarno, Mas Rizky AA TAKDIR SAILI Toha . Tya Gita Tyagita Hartady Tyagita Tyagita Uni Gamayani Wahyudin Wandi Syahindra Wibowo, Agus Wahyu Winangun, Kikin Winsa, Larasati Yulcherlina Yusuf, Alvin Zein, Syaikha Dziyaulhaq

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