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All Journal ACTA VETERINARIA INDONESIANA Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Ilmiah Mahasiswa Veteriner ZOO INDONESIA Tadarus: Jurnal Pendidikan Islam Studia Religia: Jurnal Pemikiran dan Pendidikan Islam Zoo Indonesia Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Jurnal Medika Veterinaria Jurnal Agripet
Muhammad Hambal
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Jurnal Agripet

 1 
Motilitas Ascaridia galli Dewasa dalam Larutan Ekstrak Etanol Biji Palem Putri (Veitchia merrillii) Ummu Balqis; Darmawi Darmawi; Maryam Maryam; Muslina Muslina; Abdullah Hamzah; Razali Daud; Muhammad Hambal; Rinidar Rinidar; Abdul Harris; Muttaqien Muttaqien; Azhar Azhar; Eliawardani Eliawardani
Jurnal Agripet Vol 16, No 1 (2016): Volume 16, N0. 1, April 2016
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v16i1.3022

Abstract

ABSTRAK. Tujuan penelitian ini untuk mengetahui motilitas Ascaridia galli dewasa dalam ekstrak etanol biji Veitchia merrillii. Ekstrak etanol V. merrillii dianalisis fitokimia. Sebanyak 16 ekor cacing A. galli dewasa dibagi kedalam empat kelompok. Cacing pada kelompok pertama adalah kelompok tanpa perlakuan. Cacing pada kelompok kedua diberi 0,6 mg/ml levamisole. Cacing pada masing-masing kelompok ketiga dan keempat diberi 50 dan 100 mg/ml crude ekstrak biji V. merrillii. Motilitas A. galli ditentukan dalam skor persentase setelah 12, 24, 36 jam dengan menggunakan kriteria: 3 (badan bergerak), 2 (hanya sebagian badan bergerak), 1 (tidak bergerak tetapi masih hidup), 0 (mati). Hasil fitokimia V. merrillii mengandung alkaloids, saponins, tannins, flavonoids, terpenoids. Ekstrak biji V. merrillii dosis 100 mg/ml secara in vitro dapat mempersingkat selama 12 jam waktu motilitas cacing A. galli dewasa. Penelitian ini mengindikasikan potensi anthelmintik berbasis herbal untuk pengendalian A. galli.(Motility of Ascaridia galli adult worms in vitro in ethanolic extracts of Nuts Veitchia merrillii)ABSTRACT. The purpose of this research was to know the motility of Ascaridia galli adult worms in aqueous ethanolic extracts of nuts Veitchia merrillii. The ethanolic extract of the V. merrillii was analyzed. Amount of sixteen head A. galli adult worms were divided into four groups. The first group, worms were left as un-treated normal controls. The second group, worms were treated with concentrations of 0,6 mg/ml levamisole. The third and fourth group, worms were treated with crude aqueous ethanolic extract of 50 and 100 mg/ml concentrations nuts of the V. merrillii, respectively. Motility of A. galli were determined after 12, 24, 36 hour by mean of persentage scored using the following criteria: 3 (moving whole body), 2 (moving only parts of the body), 1 (immobile but alive), and 0 (died). The result of phytochemical V. merrillii contains alkaloids, saponins, flavonoids, tannins, and terpenoids. V. merrillii nuts extract concentrations of 100 mg/ml in vitro can shorten the time motility A. galli adult worms for 12 hours. The study indicated the potential for developing herbal-based anthelmintics to control A. galli.
Peningkatan Titer Antibodi Terhadap Avian Influenza Dalam Serum Ayam Petelur yang Divaksin Dengan Vaksin Komersial Ummu Balqis; Muhammad Hambal; Mulyadi Mulyadi; Samadi Samadi; Darmawi Darmawi
Jurnal Agripet Vol 11, No 1 (2011): Volume 11, No. 1, April 2011
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v11i1.647

Abstract

Increasing of antibody titre against avian influenza in serum of vaccinated laying hens with commercial vaccineABSTRACT. The advantages of vaccination are that it reduces the risk of infection, and concurrently reduces morbidity, mortality and shedding of virus. The goal of the present study was to evaluate efficacy of Avian Influenza commercial vaccine based on humoral immunity responses of laying hens. Totally, 20 breakel silver layer hens were used in this research. The laying hens were vaccinated using Avian Influenza commercial vaccine (H5N1). Blood samples were collected from the axilaris vein (left or rigt) one time at prevaccination and repeated three times with an interval of one month at postvaccination. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that Avian Influenza commercial vaccine (H5N1) was a good protection because the vaccine was able to trigger protective humoral immunity of laying hens indicated by increasing of antibody titre in serum of vaccinated laying hens during three months.
Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli Darmawi Darmawi; Ummu Balqis; Risa Tiuria; Muhammad Hambal; Samadi Samadi
Jurnal Agripet Vol 10, No 2 (2010): Volume 10, No. 2, Oktober 2010
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v10i2.638

Abstract

Purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli L3 larvae stageABSTRACT. The main immunoglobulin fraction of poultry is called IgY, in order to distinguish it from the mammalian IgG. This article focus on purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli larvae to obtained purity IgY. Active vaccinations with excretory/secretory antigen were applied intra muscularly of chickens with an initial dose of 80 g. The vaccinations were repeated three times with dose of each 60 g with an interval of one week. The first vaccinations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by agar gel precipitation test (AGPT). The chickens eggs were collected from 49 day after vaccinations. IgY was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ekscretory/secretory IgY protein was determined by Bradford method ( = 280 nm). The result showed that antibody in yolk was begun detect with AGPT at four weeks after vaccination. IgY concentration after purification was 0,875 0.251 mg/ml. This study has shown that the product released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing antibody through yolk as biologic manufacturer could be a good choice.
Kajian Titer Antibodi Pada Yolk dari Ayam yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli Darmawi Darmawi; Ummu Balqis; Risa Tiuria; Muhammad Hambal; Samadi Samadi
Jurnal Agripet Vol 8, No 2 (2008): Volume 8, No. 2, Oktober 2008
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v8i2.611

Abstract

Evaluation of antibody titre in yolk from immunized chickens with excretory/secretory antigen of L3 stage of Ascaridia galliABSTRACT. The purpose of the present study was to trigger humoral immunity of chickens egg yolk exposed to excretory/secretory released in vitro by L3 stage of Ascaridia galli. Amount of 6 head chickens were divided into two groups. First group, the chickens were not immunized. Second group, the chickens were immunized with excretory/ secretory. Active immunizations with excretory/ secretory antigen were applied intra muscularly of chickens with an initial dose of 80 g. The immunizations were repeated three times with dose of each 60 g with an interval of one week. The first immunizations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by enzyme linked immunosorbant assay (ELISA). The result showed that antibody in yolk was begun detect with ELISA increased at two weeks after immunization, This study has shown that the excretory/secretory released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing IgY in yolk.
Perubahan Patologi Anatomi Hati dan Saluran Empedu Sapi Aceh Yang Terinfeksi Fasciola gigantica Ummu Balqis; Darmawi Darmawi; Siti Aisyah; Muhammad Hambal
Jurnal Agripet Vol 13, No 1 (2013): Volume 13, No. 1, April 2013
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v13i1.554

Abstract

Pathology anatomy changes in liver and bile duct of Aceh cattle infected with fasciola giganticABSTRACT. This study was conducted in Banda Aceh Municipality, Aceh Province to report the pathological changes in liver of Aceh cattle infected with Fasciola gigantica. In this study, livers of male aceh cattles were collected from slaughter area when Qurban in Idul Adha during two years (2011 to 2012). A total of 15 aceh cattle livers were examined. Establishment of F. gigantica were observed, and pathological changes of liver were identified. The result showed that out of 15 male Aceh cattle livers 6 (40%) were found infected with F. gigantica. The common pathological changes found in this study were the hepatic damage, and the bile ducts were changes in size and shape with the dilated lumen. The bile ducts were found filled with blackish brown exudate, which contained adult F. gigantica. In conclusion, fascioliasis is greatly responsible for hepatic damage, and currently expanding as an important veterinary public health problem.
Co-Authors . Azhar . Darmawi . Darmawi . Ismail . Muttaqien . Rasmaidar A, Aulanniam Abdul Harris Abdul Harris Abdul Harris Abdullah Hamzah Abu Hassan Ahmad Adil Umara Adistya Putra Rizki Agus Dermawan Ahmad Khamsa Fardan Al Azhar Amalia Sutriana Amiruddin A Amiruddin Amiruddin Amiruddin Amiruddin Andi Novita Anugrah Septian Anwar A Anwar A, Anwar Aprilia Wardana Arman Sayuti Arman Sayuti Arman Sayuti Atika Agusty Audi Maldini Aulanni'am, Aulanni'am Azhar Azhar Azhari A Azhari A Azhari Azhari Basuki B. Purnomo Basuki B. Purnomo Cut Dahlia Iskandar Cut S. Utami Cut Syeila Utami Darmawi D Darmawi Darmawi Dasrul Dasrul Denny Irmawati Hasan Dwina Aliza Dwinna Aliza Dwinna Aliza Elia Wardani Eliawardani Eliawardani Eliawardani Eliawardani, Eliawardani Ellyawardani Ellyawardani Elsa Mariane Ramadani Erdiansyah Rahmi Erwin Erwin Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Fadli A. Gani Faisal Mustafa Farida Athailah Farida Athaillah Farida Athaillah Farida Farida Febiola Rama Sari Fitra Aji Pamungkas Fitra Aji Pamungkas, Fitra Gholib G, Gholib Gholib Gholib Hamdani Budiman Hamdani Budiman Hamdani Budiman Hamny H, Hamny Hamny Sofyan Hasan, Denny Irmawati Henni Vanda Henni Vanda Ikwan Jamil Iraidi Muharrir Asy’ari Joharsyah J Joharsyah J, Joharsyah Khaira, Arini Ulfa Khoiruddin, Muhammad Fathul M. Aris Widodo M. Aris Widodo M. Nur Salim M. Yogi Riyantama Isjoni Maryam Maryam Muhammad Hanafiah Muhammad Hanafiah Muhammad Jalaluddin Muhammad Parwis Mulya Fahmi Mulyadi Adam Mulyadi Adam Mulyadi Adam, Mulyadi Mulyadi Mulyadi Murhaban Murhaban Muslim Akmal Muslim Akmal Muslina Muslina, Muslina Mustafa Sabri Muttaqien Bakri Muttaqien Bakri Muttaqien Bakrie Muttaqien Muttaqien, Muttaqien Nuzul Asmilia Nuzul Asmilia Qomariah, Annisa Nurul Rahmi Sulastri Mukhtar Rastina Rastina Razali Daud Razali Daud Razali Daud Razali R Razali R Razali Razali Razali Razali Resty Fauzana Rahman Retno Damayanti Soejoedono Riandi, Lian Varis Rinidar R Rinidar R, Rinidar Rinidar Rinidar Risa Tiuria Rizka Ayuni Rizki Efriyendi Roslizawaty r Roslizawaty R Ruskhanidar R, Ruskhanidar Rusli Rusli Rusli Rusli Saddat Nasution Saddat Nasution, Saddat Samadi Samadi Sari, S.Si, M.Si, Wahyu Eka Sari, Wahyu Eka Sembiring, Rinawati Siti Aisyah Siti Aisyah Sri Wahyuni Sri Wahyuni Sugito - Sugito Sugito Sugito Sugito Suriadi S Suriadi S, Suriadi Sutiman B. Sumitro Syafruddin s Syafruddin S Syafruddin S Syifa Salsabila T Reza Ferasyi T. Armansyah T. Armansyah T. Fadrial Karmil T. Reza Ferasyi Tapielaniari - Taufikah Rahmah Teuku Reza Ferasyi Teuku Reza Ferasyi, Teuku Tongku Nizwan Siregar Triva Murtina Lubis Ummu Balqis Winaruddin Winaruddin Wulan Windasari Yolanda Sari Yudha Fahrimal Yudha Fahrimal Zainuddin Z Zainuddin Z Zainuddin Zainuddin Zakiah Heryawati Manaf Zakya Ardi Zamzami, Rumi Sahara Zuhrawati Z Zuhrawaty NA Zuhrawaty Zuhrawaty Zulfayanto, Indri Zulkifli, Baidillah