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All Journal Jurnal Veteriner Media Kedokteran Hewan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Medik Veteriner Darmabakti Cendekia: Journal of Community Service and Engagements Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology GUYUB: Journal of Community Engagement Mitra Mahajana: Jurnal Pengabdian Masyarakat LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Bima Abdi: Jurnal Pengabdian Masyarakat

Tri Wahyu Suprayogi

Study Program Of Veterinary Paramedic, Department Of Health, Faculty Of Vocational Studies, Universitas Airlangga, Surabaya-Indonesia Department Of Veterinary Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya-Indonesia

Author-ID : 2866056

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The addition of duck egg yolk in skim milk – fructose extender maintains sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage Isti Annisa Ramahtia; Tatik Hernawati; Tri Wahyu Suprayogi; Tjuk Imam Restiadi; Djoko Legowo; Samuel Inioluwa Akeju
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.1-8

This study aimed to determine the addition of duck egg yolk in skim milk – fructose extender on sperm motility and viability of Muscovy duck (Cairina moschata) at 5°C storage. Ejaculates of Muscovy duck were divided into four portions equally. The first portion was for the control group (diluted in skim milk – fructose extender), and the second, third, and fourth portions were for the treatment groups of T1, T2, and T3 (diluted in skim milk – fructose extender added with 2.5, 5.0, and 7.5% v/v duck egg yolk). Sperm motility and viability were evaluated two hourly until the motility percentage reached 40%. The data were analyzed using ANOVA and Duncan test. The result showed that the highest percentage of spermatozoa motility and viability (p<0.05) was in the T2 group compared to the other groups. It could be concluded that the Muscovy duck semen extended in skim milk - fructose with 2.5 to 7.5% egg yolk of duck qualified for artificial insemination at storage up to 10 hours in 5°C storage.

The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.

Coconut water combined with purebred chicken egg yolk as an alternative semen extender for Madura Pote bucks semen preservation Tatik Hernawati; Susilowati, Suherni; Tri Wahyu Suprayogi; Widya Paramita Lokapirnasari; Zulfi Nur Amrina Rosyada
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.96-104

One of the many efforts to increase the quality of livestock genetics is through artificial insemination (AI). Other than increasing it, AI can be conducted to preserve semen. A successful AI is determined by seminal quality, therefore, a method to preserve semen for a longer storage time is needed. The method used is adding an extender that fulfills prerequisites for a semen extender such as coconut water combined with egg yolk citrate extender. Coconut water is rich in carbohydrates, protein, vitamins, and antioxidants while egg yolk contains lecithin. This study aims to find out the Pote buck spermatozoa quality stored in coconut water and egg yolk extender. This study uses three groups of treatments (T0: 0.1 ml semen + 0.9 ml egg yolk citrate, T1: 0.1 ml semen + 0.9 ml coconut water, and T2: 0.1 ml semen + egg yolk citrate (20%) + coconut water). All three of these are stored at 5oC and evaluated every day until day 5 of their motility, viability, intact plasma membrane, abnormality, and MDA level. Data analysis used is ANOVA and a further test called BNT is conducted if a significant difference is determined. No significant difference was found between T0 and T1 (p>0.05). The highest progressive motility, viability, and intact plasma membrane (%) among the three groups of treatments happened to be from T2. Meanwhile, a low percentage of spermatozoa abnormality and MDA level were also found in T2 with its extender being coconut water combined with egg yolk citrate. To conclude, the best extender for storing Pote buck semen is stored at 5oC is coconut water combined with egg yolk citrate extender.

Spermatozoa motility and viability of turkey (Meleagris gallopavo) semen in egg yolk nanoparticles-phosphate buffered saline extender at 3-5°C storage Nabila Ayu Saraswati; Herry Agoes Hermadi; Tri Wahyu Suprayogi; Tatik Hernawati; Eka Pramyrtha Hestianah
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.129-138

This study aimed to evaluate the spermatozoa motility and viability of turkey semen extended with egg yolk nanoparticles-PBS extender at various concentrations, stored at 3-5°C. Semen was collected from two turkeys and divided into four treatment groups. Group T0 consisted of fresh turkey semen extended with 20% (v/v) fresh egg yolk in phosphate-buffered saline (PBS). Groups T1, T2, and T3 consisted of fresh turkey semen extended with 5, 10, and 20% (v/v) egg yolk nanoparticles in PBS, respectively. Extended semen was stored in a refrigerator (3-5°C), and progressive spermatozoa motility and viability were evaluated every two hours until spermatozoa motility decreased to 40%. The results showed that turkey semen in T3 extender containing 20% egg yolk nanoparticles in PBS was able to maintain spermatozoa motility for up to eight hours, better than T0 and T2 extenders which supported spermatozoa motility for six hours. In contrast, T1 extenders only support spermatozoa motility for four hours. T3 extenders were also able to maintain spermatozoa viability for up to eight hours, while T0, T1 and T2 extenders could only support viability for 6 hours. The extender with 20% egg yolk nanoparticles in PBS was significantly better (p <0.05) in maintaining spermatozoa quality compared to those with 5 and 10% egg yolk nanoparticles, as well as the extender with 20% fresh egg yolk. In conclusion, 20% egg yolk nanoparticles in PBS semen extender was effective in maintaining the motility and viability of turkey (Meleagris gallopavo) spermatozoa stored at 3-5°C for up to eight hours.

Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.

Ethanolic extract of Dayak onion (Eleutherine palmifolia) prevented sperm membran damage in mice exposed to monosodium glutamate Syahraini Adhiya Lathifah; Aldin Akbar Rahmatullah; Boedi Setiawan; Chairul Anwar Nidom; Nove Hidajati; Sri Mulyati; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.153-160

Monosodium glutamate (MSG) could cause increased production of reactive oxygen species (ROS), causing oxidative stress in the testicles, which adversely affected sperm quality. Dayak onion extract which is known for its high antioxidant content, could help alleviate oxidative stress caused by MSG. This research examined the effect of Dayak onion (Eleutherine palmifolia) on sperm abnormalities and plasma membrane integrity in mice (Mus musculus) exposed to MSG. Twenty-five male mice, 8 weeks old with a body weight approximately 20g, were divided into five groups, C– (received 0.5% Na-CMC), C+ (received 4 mg/g bw MSG), and three treatment groups (T1, T2, T3) which were given 4 mg/g bw MSG accompanied by Dayak onion extract at doses of 30, 60, and 120 mg/kg bw respectively. All treatments lasted for 52 days. Significant differences (p <0.05) in sperm abnormalities and plasma membrane integrity were observed among the groups. Sperm abnormalities found were (12.3 ± 1.92), (61.1 ± 3.10), (41.6 ± 2.87), (30.4± 1.91), and (18.2 ± 2.10) % respectively for C–, C+, T1, T2 and T3. Meanwhile, sperm plasma membrane integrity found were (33.0 ± 3.24), (69.3 ± 2.32), (41.8 ± 2.42), (55.4 ± 3.11), and (64.2 ± 1.27) % respectively for C–, C+, T1, T2 and T3. These results indicate that Dayak onion extract could help reduce sperm abnormalities and maintain plasma membrane integrity in mice exposed to MSG.

CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI PERANAKAN LIMOUSIN DAN SAPI PERANAKAN ONGOLE HASIL IB DI KECAMATAN PILANGKENCENG KABUPATEN MADIUN Bhaskara, Alfiutha Propha; Suprayogi, Tri Wahyu; Darsono, Roesno; Srianto, Pudji; Hariadi, Mas'ud; Sardjito, Trilas
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The study was conducted to know conception rate and service per conception of Limousin Cross breed and Ongole Cross Breed in Pilangkenceng, Madiun. Data were collected for the periode of Oktober 2016 to February 2017 from inseminators in Pilangkenceng district. The data were taken by primary and secondary. Primary data retrieval were done by direct observation, which includes several variables, where the variables include: the identity of the breeder, feeding and drinking. The secondary data were obtained by recording the farmer's artificial insemination card. All data was analyzed to find a percentage of conception rate (CR) and find number of service per conception (S/C). Then S/C were analyzed with T-test adn CR were analyzed with chi-square test. The results of this research showed that the service per conception's average of limousin cross breed and ongole cross breed is 1,61 and 1,72. The average of conception rate limousin cross breed and ongole cross breed is 53% % and 59 %. The result showed no significant difference (p>0,05) on CR and S/C between limousin cross breed and ongole cross breed. The conclusion of this research showed that service per conception and conception rate of ongole cross breed in Pilengkenceng Madiun is better than limousin cross breed but there's no significant difference (p>0,05).

PENGARUH PEMBERIAN EKSTRAK KULIT BAGIAN DALAM SEMANGKA (Citrullus lanatus) TERHADAP KEUTUHAN MEMBRAN PLASMA DAN ABNORMALITAS MORFOLOGI TIKUS JANTAN (Rattus norvegicus) SETELAH DIPAPAR SUHU PANAS Reisinta, Dinda; Paramita L, Widya; Hernawati, Tatik; Meles, Dewa Ketut; Madyawati, Sri Pantja; Suprayogi, Tri Wahyu
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

This study aims to determined the effect of watermelon's inner rind extract (Citrullus lanatus) towards the integrity of the plasma membrane and morphological abnormalities of male Norway rats's spermatozoa (Rattus norvegicus) after being exposed to hot temperature. The content of lycopene in the inner rind of watermelon serves an antioxidant counteracting free radicals. A total of 20 male Norway rats (Rattus norvegicus) were used in this study with an average age of 2 months. The study was divided into five treatments and four replications, consisting of: K(-) as negative control given CMC Na 0.5% as much as 0.5 ml, P0 as positive control was exposed to 40° C for 1 hour and CMC Na 0.5% of 0.5 ml. P1, P2 and P3 are exposed to 40° C for 1 hour and inner rind extracts of watermelon with a multilevel dose of P1: 20mg/head/day, P2: 40mg/head/day, and P3: 80mg/head/day that each dose was dissolved in CMC Na 0.5% of 0.5 ml. The results showed that giving inner rind extract of watermelon to the rats increased the integrity of the plasma membrane and decreased morphological abnormalities of male rat's spermatozoa (Rattus norvegicus). The result of increased the integrity of the plasma membrane and highest decreased morphological abnormality was shown by P3 group given a dose of 80 mg/head/day.

PENGARUH PENEMBAKAN LASERPUNKTUR PADA TITIK REPRODUKSI ITIK CAMPBELL (Anas platyrhynchos domesticus) BETINA TERHADAP PRODUKTIVITAS TELUR Renata, Sena Sangga; Suprayogi, Tri Wahyu; Estoepangestie, A.T Soelih; Adikara, R.T. Santanu; Tehupuring, Benjamin Chr.; Hidanah, Sri
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The technology of laserpuncture in livestock is an acupuncture technique using Laser (Light Amplification by Stimulated Emission of Radiation) that is fired at acupuncture points as biological receptors that are related to organs that can provide stimulatory effects. This research was conducted in the laboratory of Airlangga University for 30 days using a sample of 18 cambell ducks divided into 3 treatments and laser shot with 3 days interval. Treatment 0 as control, treatment 1 got laser treatment dose 0,2 joule and treatment 2 got laser treatment dose 0,5 joule. On the final result of the eggs that have been in the production of duck research for 29 days. P2 given laser shootings with a dose of 0.5 joule yielded more eggs with 158 grains compared to P1 given laser shootings with a dose of 0.2 joules yielding 126 grains and P0 with inactivated laser yielding a total of 86 grains. The conclusion of this study is the shooting of laserpuncture dose of 0.2-0.5 joule can increase egg productivity

HUBUNGAN ANTARA BODY CONDITION SCORE (BCS) DENGAN PRODUKSI SUSU SAPI PERAH FRIESIAN HOLSTEIN (FH) Netika, Melsa; Darsono, Roesno; Utomo, Budi; Mustofa, Imam; Ismudiono, Ismudiono; Suprayogi, Tri Wahyu
Ovozoa: Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The aim of this research was to observe the Body Condition Score (BCS) and milk production of Friesian Holstein (FH) dairy cattles. 144 of dairy cattles divided into 3 groups based on BCS 1-9 scale. The results showed that Y = 6.62 + 5.54X - 5.78X2. Coefficient of determination (R2) of 20.6 this mean any increase in milk production by 20.6% is affected by BCS. It can be concluded that there is a correlation between Body Condition Score (BCS) and milk production. The higher BCS (BCS 7-8) the lower milk production, while in ideal BCS production (5-6) the production of milk is optimize.

Co-Authors Abdul Samik Abdul Samik, Abdul Adikara, R.T. Santanu Aditya Irawan Aditya Sukma Pawitra Agung Budianto Achmad Agus Widodo Agustono, Bodhi Ahmad Thoriqul Firdaus Al Arif, M Anam Aldin Akbar Rahmatullah Aliyah, F. Himmatul Amaliya, Anny Amrullah, Muhammad Fajar Ani Wijayanti Anisa, Nabila Berlianny Nur Annisa Nur Fitria Rahma Anwar Ma'ruf Anwar Ma'ruf Ardina Sahra Miranda Ardiyansyah, Septiana Alafia Yusi Arini, Mirza Asafaizh Astrid Annisya Adhy Putri Aswin Rafif Khairullah Ayudani, Saskia Nadhira Az'zahroh, Na'ilah Putri Azhari, Akbar Maulana Belgania, Ristaqul Husna Bhaskara, Alfiutha Propha Boedi Setiawan Brahmantya, Chandra Brillianti, Febby Fairy BUDI UTOMO Budi Utomo Candika, Yossy Imam Dadik Rahardjo, Dadik Darsono, Roesno Deskayanti, Asih Dewi, Aprilia Kurnia Dewita Dewita Dikman, Dicky Mohammad Djoko Legowo Djoko Legowo Dwi Novita Sari Eduardus Bimo Aksono Herupradoto Eduardus Bimo Aksono Herupradoto Eka Pramyrtha Hestianah Epy M. Luqman Erma Safitri Estoepangestie, A.T Soelih Fajar Ramadhan Subiyantoro Febriani, Nur Rahma Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Heni Puspitasari Herinda Pertiwi Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidanah, Sri Hidayatik, Nanik Himatul Oktavia Imam Mustofa IMAM MUSTOFA Indah Norma Triana, Indah Norma Iskandari, Novia Nur Ismudiono Ismudiono Ismudiono Ismudiono, Ismudiono Isti Annisa Ramahtia Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Khoirunnisa, Itrah Khuliyah Candraning Diyanah Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Lilik Maslachah Listyorini, Linda Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Lusiandari, Ria Masyitoh, Herlina Meles, Dewa Ketut Mirni Lamid Mufasirin Muhammad Ridwan Muhammad Thohawi Elziyad Purnama Muhammad Yunus Mustofa Helmi Effendi Nabila Ayu Saraswati Netika, Melsa Nidom, Chairul Anwar Nove Hidajati Nurdianto S., Ricko Nusdianto Triakoso Nusdianto Triakoso octavia, Arinda Oky Susandani Palupi, Tantri Dyah Whidi Paramita L, Widya Poedji Hastutiek Praja, Ratih Novita Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama, Abrian Panggalih Indra Pratomo, Andrika Indra Pristafania, Triana Pudji Srianto Rahadi, Yustisiane Ruth Rahmatullah, Aldin Akbar Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ratna Damayanti Reisinta, Dinda Renata, Sena Sangga Restiadi, Tjuk Imam Retno Sri Wahjuni Rifky Nugroho Rimayanti Rimayanti Ristiani, Widya Aulia Rochmah Kurnijasanti Rochmi, Siti Eliana Rofiudin, Muhammad Ilham Rosyada, Zulfi Nur Amrina Safitri, Erma - Samuel Inioluwa Akeju Saputra, Ardan Saputra, Rakan Mahiid Saputro, Amung Logam Seagames Waluyo, Seagames Setyawan, Firman Sherina Lashita Candrakirana Sosilowati, Suherni Sri Agus Sudjarwo Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suharti Aryanata Suherni Susilowati Suherni Susilowati Suhita Aryaloka Sulistyowati, Deny Sunarso, Agus Supriyadi Supriyadi Supriyadi Suzanita Utama Syahraini Adhiya Lathifah Syuhuud Arumbinang Wajdi, Syuhuud Arumbinang Tatik Hernawati Tehupuring, Benjamin Chr. Tita Damayanti Lestari Tjuk Imam Restiadi Tri Bhawono Dadi Trilas Sardjito Ulfah, Norma Amalia Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widjiati Widjiati, Widjiati Widodo, Oky Setyo Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yossy Imam Candika

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