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Growth Analysis of Single Cell Protein (PST) Bacteria Bacillus cereus using Different Media Mutiara Naimi Nasution; Feliatra Feliatra; Irwan Effendi
Jurnal Perikanan dan Kelautan Vol. 26 No. 1 (2021): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.1.47-53

Abstract

The Media is a material consisting of a mixture of nutrients used by microorganisms to grow and breed. Molasses and liquid waste know one of the alternative media which has the least inexpensive carbon source and is easily obtained from the waste food industry that is rich in nutrients and minerals, so it has the potential for bacterial growth of Bacillus cereus. The consetration of molasses used for growth medium is 1%; 1.5%; and 2% while liquid waste knows 8%, 10%, and 12%. Cell growth is measured by the method of Spectrophotometry, total plate count (TPC) and biomass while the incubation period of cell growth is measured every 0, 6, 12, 18, and 24 hours. The results showed from the molasses + skim milk and the tofu liquid waste + skim milk, the best medium of bacterial cell growth of B. cereus is a liquid waste know isolates K 12%. While the best growth concentrations molasses Medium bacterial B.cereus was N2% and K2%.
IMPLEMENTATION OF CONSORTIUM BACTERIES OF Bacillus cereus ISOLATED FROM SEA AS PROTEIN SOURCE FOR PRO COFFEE BEVERAGE Zevira, Bella Permata; Feliatra, Feliatra; Batubara, Ummi Mardhiah
Asian Journal of Aquatic Sciences Vol. 7 No. 3 (2024): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.7.3.378-385

Abstract

Pro coffee is a fermented beverage using Bacillus cereus bacterial consortium with single-cell protein. This study aims to determine the best composition of the B. cereus consortium bacteria mixture in organoleptic aspects that panelists most prefer and the effect of adding B. cereus consortium bacteria on proximate analysis and pro coffee flavor. Organoleptic testing of probiotic coffee was conducted on 20 panelists, then continued with proximate analysis of pro coffee. The composition with the best organoleptic flavor is obtained in code D, with the treatment of adding 5% bacterial consortium B. cereus for 15 days. The analysis of taste obtained has a value of 0.000 <0.05, and the study results show that the significance value is smaller than the significance level. Hence, the treatment results give the effect of adding bacterial consortium to pro-coffee. The addition of bacterial consortium B. cereus to probiotic coffee, in the form of moisture, ash, protein, fat, and carbohydrate content, is 0.000 <0.05. The analysis results show that the significance value is greater than the significance level, so the treatment results do not give the effect of adding bacterial consortium to pro coffee
ISOLATION OF LACTIC ACID BACTERIA FROM VANAME SHRIMP (Litopenaeus vannamei) AND ITS ANTAGONISM TO PATHOGEN BACTERIA Sufaidah, Siti Nur; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol. 7 No. 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Vaname shrimp is one of Indonesia's leading export species and is widely cultivated. The main problem in vaname shrimp pond cultivation is the attack of shrimp diseases caused by bacteria or viruses. This study aims to isolate and identify lactic acid bacteria (BAL) from vaname shrimp (Litopenaeus vannamei) and test their antagonism against pathogenic bacteria. The research was conducted from February to April 2023. The method used in this study is a survey method. Sampling was conducted at the vaname shrimp pond of the Fisheries Service Task Unit (UPT), Tanjung Punak Village, Rupat Utara District, Bengkalis Regency, Riau. The method used in this research was a survey method. BAL was isolated using MRS Agar + CaCO3 media. The Kirby-Bauer method was used to test bacterial antagonism against pathogenic bacteria. Based on the identification of seven isolates, it was found that only four isolates were positively identified as LAB bacteria, namely 47D, 54T1, 73DB1, and 73BD2. The results of the antagonism test against pathogenic bacteria showed that all BAL isolates could not suppress the growth of E. coli bacteria. However, these isolates suppressed the growth of A. hydrophila bacteria, with the widest inhibition zone diameter on isolate 54T1 (4.2 mm). On Vibrio sp. bacteria, the widest inhibition zone of BAL was on isolate 73DB1 (6 mm). These results indicate that the antibacterial activity produced by BAL is still relatively weak.
BIOMASS PRODUCTION AND SINGLE-CELL PROTEIN (SCP) ENCAPSULATION OF BACTERIA Bacillus cereus SN7 Mukramin, Ibnu; Feliatra, Feliatra; Tanjung, Afrizal
Asian Journal of Aquatic Sciences Vol. 7 No. 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Bacillus cereus SN7 is a bacterium with potential applications in producing Single-Cell Protein (SCP). SCP can be an alternative to protein derived from traditional sources like agriculture, fisheries, and animal husbandry. Encapsulation is a process used to stabilize cells, aiming to potentially enhance probiotics' viability and stability throughout production, storage, and handling. This study aims to make capsules from B. cereus SN7 single-cell protein to protect them from contaminants. This study used a survey method, which carried out in-situ processing of bacteria, as well as conducting experiments on the formation of bacterial encapsulation, testing the effectiveness of encapsulation, and observing the viability of B. cereus SN7 bacteria by counting the number of colonies. B. cereus produced biomass with a total dry weight of 1.3708 g/mL. The alginate encapsulation experiments can be formed into capsule beads that protect bacteria. The best encapsulation process is an alginate-based coating, which is indicated by the ability of the alginate to withstand temperature, pH, and salinity.
OPTIMIZATION OF Bacillus cereus GROWTH IN MEDIA WITH DIFFERENT CARBON SOURCES Putra, Muhammad Hendra; Feliatra, Feliatra; Effendi, Irwan
Asian Journal of Aquatic Sciences Vol. 4 No. 3 (2021): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Bacillus cereus is a probiotic bacteria that requires media as a source of nutrients for its growth. This study aims to the media used were tofu liquid waste, sago liquid waste, and molasses. The method used is an experimental method, namely testing two groups of bacterial isolates; B. cereus SN7 and the consortium (combination of five isolates from B.cereus with different strains). Each group of isolates was grown with three replications on each waste medium. bacterial growth every six hours for twenty four hours using two methods; Total Plate Count (TPC) and bacterial cell biomass. There was no significant difference in growth between the two groups of bacterial isolation. The optimal growth of each isolate was found in molasses waste. The growth between two bacterial isolates in the liquid waste media was B. cereus SN7 bacterial isolate.
ABILITY OF AMILOLYTIC BACTERIA (Bacillus paramycoides AND Enterobacter cloacae) IN DEGRADING ORGANIC MATERIALS OF MANGROVE LITTLE Putri, Redila Aprilivia; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol. 4 No. 2 (2021): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This study aims to find out that Bacillus paramycoides and Enterobacter cloacae bacteria can produce amylase enzymes and have the ability to degrade organic matter, especially mangrove litter. From this study it was found that the optimal growth of B.paramycoides and E. cloacae bacteria occurred at 12th hour. The results of measurements and calculations of absorbance values ​​at 630 10.238 x 108 cells/mL (B. paramycoides) and 12.030 x 108 cells/mL (E. cloacae) using the spectrophotometric method. Meanwhile, with the TPC method at 12 hours, the number of bacterial cells was 2.08 x 108 CFU's/mL (B.paramycoides) and 2.44 x 108 CFU's/mL (E. cloacae). The ability to produce the largest amylolytic bacterial amylase enzyme also occurred at 12 hours as much as 0.306 mg/mL (B.paramycoides) with an increase of 0.046 mg/mL and 0.243 mg/mL (E. cloacae) with an increase of 0.028 mg/mL. The bacteria that have the highest amylase enzyme ability is E.cloacae as evidenced by the diameter of the clear zone of 10.10 mm. Testing the ability of amylolytic bacteria in degrading mangrove litter was carried out by adding amylase enzyme as much as 0%, 50% and 100%. Amylolytic bacteria can degrade organic matter by hydrolyzing starch contained in mangrove litter. The most degraded starch content was in the 100% enzyme treatment, which was 1.021 mg/mL (B. paramycoides) and 1.189 mg/mL (E.cloacae).
SECONDARY METABOLITE CHARACTERISTICS OF HETEROTROFIC BACTERIA PRODUCTION AS ANTIMICROBIAL AT DIFFERENT SALINITY Apriyola, Nadya; Feliatra, Feliatra; Nurulita, Yuana
Asian Journal of Aquatic Sciences Vol. 3 No. 2 (2020): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research was conducted from March-June 2019. The purpose of this study was to determine the characteristics of secondary metabolites prduced by of heterotrophic bacterial from sea water Sungai Kayu Ara Village, Siak Regency as an antimicrobial and to determine the storage time of these bacteria by measuring at the optimal growth time. Five bacterial secondary metabolite extracts used were B, C, D, and H (B. cereus) and J (V. fluvialis) obtained from the collection of Marine Microbiology Laboratory, Department of Marine Sciences, Faculty of Fisheries and Marine sciences, University of Riau. phytochemical test showed that extracts of isolates B, D, and H contained saponin compounds, while isolate J contained flavonoid compounds, however, all extracts contained alkaloid compounds. Antimicrobial test indicated that J extract inhibited A. Hydrophila at concentration 500 µg/ml but the extract could not inhibit V. algynolyticus and Pseudomonas sp concentrations determined. In the bacterial storage time test, the optimal growth of each bacterial concentration at was 7th day incubation and decreased on the 14th day.
MOLECULAR IDENTIFICATION OF PROTEOLITIC BACTERIA FROM MANGROVE SEDIMENT IN DUMAI MARINE STATION Zebua, Abdi Hikmat Petra; Nursyirwani, Nursyirwani; Feliatra, Feliatra
Asian Journal of Aquatic Sciences Vol. 3 No. 2 (2020): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Proteolytic bacteria have important role in the degradation of complex compounds to simple compound by the enzyme proteases. Sediment in mangrove ecosystem of Dumai Marine Station may contain the bacteria. This research aims to identify the proteolytic bacteria of mangrove sediments molecularly and to examine the antagonism of pathogenic bacteria. The method used in this research was survey. The results obtained 10 bacterial isolates (AZ1, AZ2, AZ3, AZ4, AZ6, AZ10, AZ11, AZ15, AZ18, and AZ20). Identification using 16S rRNA analysis revealed that 3 isolates showed different results, namely AZ2 had a similarity to Bacillus proteolyticus with strain MCCC 1A00365, AZ6 had a similarity to the bacteria B. monlinensis with strain BL4-6 and AZ20 had similarity to the bacterium B. toyonensis with strain MCCC 1A00365 BCT-7112. Three of the 10 isolates that had the highest inhibition against pathogens. Isolates AZ2 shovued inhibition zone against Pseudomonas aeruginosa with a diameter of 9.65 mm, AZ6 shovued inhibition zone against Escherichia coli bacteria with a diameter of 5.18 mm, and AZ20 shovued inhibition zone against Vibrio alginolyticus bacteria with a diameter of 4, 01 mm.
OPTIMIZATION OF THE GROWTH MEDIA OF Bacillus cereus BACTERIA WITH THE ADDITION OF SKIM MILK AND DIFFERENT CARBON SOURCES Taufan, Muhammad; Feliatra, Feliatra; Effendi, Irwan
Asian Journal of Aquatic Sciences Vol. 5 No. 1 (2022): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The goal of this research was to find the optimal media for growing B. cereus bacteria with various carbon sources. This study used an experimental method with two Bacillus cereus bacterium isolates, namely B. cereus strain SN7 (isolate N) and a consortium of bacteria isolates (a combination of several B. cereus isolates with different strains). The two isolates were grown in sago liquid waste media, tofu liquid waste media, and molasses liquid waste media supplemented with a protein source, skim milk, with three replications in each treatment. Bacterial culture growth was measured every 6 hours for 24 hours using two methods: the TPC method and bacterial cell biomass measurement. According to the results of this study's measurements, molasses waste media was the best modified medium for B.cereus growth. The difference in growth between the B. cereus strain SN7 bacterial isolates and the consortium cultured on molasses media was not significant. The isolates with the largest number of cell growth were B. cereus consortium isolates, with 2,023 x108 CFU/mL in the TPC method and 1,011 g/mL in the biomass measurement method at 24 hours.
Pemberdayaan Kelompok Masyarakat Nelayan untuk Pengembangan Olahan Hasil Tangkapan Ikan di Desa Bukit Batu Kabupaten Bengkalis Nursyirwani, Nursyirwani; Feliatra, Feliatra; Siregar, Yusni Ikhwan; Batubara, Ummi Mardhiah
Journal of Rural and Urban Community Empowerment Vol. 5 No. 2 (2024): April
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (802.33 KB)

Abstract

Desa Buruk Bakul yang terdapat di wilayah pesisir Pulau Sumatera merupakan penghasil ikan laut di daerah Kabupaten Bengkalis Provinsi Riau. Beberapa jenis ikan ekonomis penting seperti ikan terubuk (Tenualosa macrura), tenggiri (Scomberomorus commerson), debuk (Arius thalassinus), biang (Septipina sp), dan lomek (Harpodon nehereus) diperoleh dari hasil tangkapan nelayan di desa tersebut. Ikan-ikan tersebut biasanya dijual dalam bentuk segar, namun jika tidak habis terjual, sebagian hanya diolah menjadi ikan kering dan kerupuk ikan. Kegiatan pengabdian kepada masyarakat (PKM) ini bertujuan untuk meningkatkan keterampilan kelompok Ibu Rumah Tangga (IRT) nelayan di Desa Bukit Batu dalam mengolah ikan-ikan seperti ikan tenggiri menjadi produk yang lebih bervariasi seperti bakso, nugget dan stik ikan. Program ini dilakukan dalam tiga tahapan, yaitu (1) pendekatan dan sosialisasi program, (2) pelatihan dan peningkatan kompetensi, dan (3) pendampingan kelompok, monitoring dan evaluasi. Kegiatan PKM melibatkan 25 IRT nelayan (60%) dan ibu-ibu rumah tangga lainnya. Pelaksanaan kegiatan juga dibantu oleh 10 orang mahasiswa KKN Terintegrasi UNRI 2023. Berdasarkan kegiatan PKM diperoleh hasil adanya peningkatan pengetahuan dan keterampilan peserta (85%) setelah mengikuti pelatihan tentang teknik diversifikasi olahan ikan. Hasil monitoring dan evaluasi menunjukkan bahwa 100% peserta program menyatakan program PKM sangat bermanfaat. Dari analisa keberlanjutan program menunjukkan bahwa terdapat 2 jenis pelatihan yang diinginkan oleh peserta, yaitu (1) pengolahan jenis olahan berbasis ikan yang lain, dan (2) pembuatan rancangan kemasan produk.
Co-Authors ', Baihakki A. Momang Yusuf A.J. Patandean AA Sudharmawan, AA Aan Deni Masrudy Abd. Rasyid Syamsuri Abdul Hadi Abdul Halim Talli Abdul Haris Adid A Hermansyah Adli Abdullah, Muhammad Adolf Hiskia Nainggolan Afifah, Zulfa Afriansyah Anca Afrizal Tanjung Agung EBW Agus Sevtiana Agustinus R. Uria Ahmad Asyrofi Ahmad Asyrofi, Ahmad Ahmad Yani Aini, Vera Nur Ainul Ghurri Alberto Taolin Aldri Frinaldi Alfarisy, M. Phyto Almahmudy, Muhammad Naufal Alviccenia Lailatul Mukaromah Aminatul Farihah Anak Agung Istri Sri Wiadnyani Anas Anas, Anas Anas Zubair, Anas Andi Ahmar Ihwal Ikhsan Andi Dahliaty Andrei Putra Zirma Andrito, Wahyudi Anggi Rohita Anggun Meita Anggraini Anisa Qoriman Anita R Sidabutar Anita Rachmasari S.W. Anto Budi Listyawan Apriliani Indrawati Putri Apriyola, Nadya Ari yanto rama doni Arief Widodo Arif Rahman Bachar, Sutan Bagus Dwi Hari Setyono Batubara, Ummi Mardhiah Bintal Amin Birmanti Setia Utami Buchari Buchari Bustari Hasan Cokorda Istri Raka Marsiti Cut Murzalina Cut Zahara Phoenna Dahliaty, Andy Damar Adhiguna Daulay, Anita Diah Permata Sari Defa Jeni Nariswari, Defa Jeni Desi Anggreni Dessy Yoswaty Dessy Yoswaty Devi Ambarwaty Oktavia Devi Ambarwaty Oktavia Devi Irma Sucia Ningtyas Dewan Pratama Novian Dewi Musawwanah Dewi S. Zilda Dewi Sujani Adithiya Dewi, Shinta Rafida Dharmaputra Palekahelu Dian Agung Wicaksono Dian Iriani Dini Suwandari Diva Cicilya Nunki Arun Sudibya Diva Cicilya Nunki Arun Sudibya, Diva Cicilya Djoko Mursinto, Djoko Dora Darussalam Eka Ahadiyat Suryana Eka Ardhianto Elisa Paramita Ema Komalasari, Ema Epta Dian, Ni Kadek Ayu Erlangga Andi S ERMAN ERMAN Euis Sri Damayanti Euis Sri Damayanti Eza Buana Fatwa Eza Buana Fatwa Fatmanita Fatmanita Ferenza, Oktovalen Fitri Widiantini Fitriyani, Fara Ghazali, Tengku Muhammad Gustina, Julia Gustina, Lenny Hafid Abdullah Hafid Hafid Hana Ilmi Khoiriyah Handayani, Sunita Hanif Muchdatul Ayunda Hanifah Yusuf Hariyono, Widodo Hartaman, Nursaleh Hasan, Fitri Utami Hasyimi, Wahid Hayani Anastasia Hendra Adi Prasetya Heru Noviat Herdata Hilyati Fadhlah I Wayan Hendra Saputra I Wayan Supardi Ibnu Khaldun Ida Ayu Putu Hemy Ekayani Iesje Lukistyowati Iesje Lukistyowati Iesje Lukystiowati Ilham Fajri Pohan Intan R Mutiaz Irfan Dwidja Priyambada, Irfan Dwidja Irvina Nurachmi Irwan Effendi Irwan Irwan Ismail Abdurrahman Siregar Jasanta Peranginangin Joni Dwi Pribadi Joni Dwi Pribadi Junaidi, Muhammad Kaharuddin Arafah Kamsinah Kamsinah Karnila, Rahman Karnila Kartika Indah Permanasari Kartika Yuliari Ketut Aswata Khotibul Umam Kristin Wahyuningsih Kristin Wahyuningsih, Kristin Kumala Dewi Kurniati Laksono Trisnantoro Lamun Batara Layla Tun Nur Lazuardi Lazuardi Lidia Dinawati Lora Lestari Lubis Lora Lestari Lubis Lukystyowati, Isye M, Godras Jati Made Agus Nurjana Made Vivi Oviantari Mardalisa, Mardalisa MARIA BINTANG Mariska Lauterboom Marpaung, Franky Hadinata Masdini, Alvira Mayda Susana Meliza Rahmi Moch. Cahyo Sucipto Mochamad Solikhin Mohamad Thahir Haning Mojang Al Mukaromah Muhammad Agus Muljanto Muhammad Fuad Zaini Muhammad Isa Muhammad Muhammad Muhammad Nur Sahid Muhammad Setya Pratama Muhammad Taufan Mukramin, Ibnu Muliana Muliana MUSLIMIN IBRAHIM Mutiara Naimi Nasution Muttaqin Mansur, Teuku Nadhia Agustina Nanang Tri Prastyo Nasrullah Nasrullah Nasution, Mutiara Naimi Ni Nyoman Veridiana Ni Putu Nita Anggraini Nia M. Kurnia Nittaya Chaiyanate, Nittaya Nobelina Adicondro Nobelina Adicondro Nofriyani Nofriyani Noladhi Wicaksana Nono Carsono Nosilia Fristiani Nur Aisah Amini Nur Indrayati Nur Indar Nurfauziah, Suci Nurmalita Putri Nugrahena Nursit Wijayanto Nursyirwani, Nursyirwani Nursyiwani Nursyiwani Nurzamli Nurzamli Nyoman Wendri Oktovalen Ferenza P Purwanto Paramita, Elisa Pertiwi, Fenti Dewi Pipin Kurnia Pipin Kurnia Popy Wardani Prasetya, Hendra Adi Purwoko, Agus Putra, I Ketut Suanjaya Adi Putra, Muhammad Putra, Muhammad Hendra Putri Ayu Putri, Redila Putri, Redila Aprilivia Rahmanda, Melysa Ranap Junani Sari Rena Feri Wijayanti Rena Feri Wijayanti Rendy Lani Dinata Rezki Aitul Hukmi Rifa Fauzi Rosmerry Rifaldi Rifaldi, Rifaldi Rifardi Rini Darmastuti Rintawati Sandradewi, Rintawati Riski Gusri Utami Rizki Hamdani Rohula Utami Rozi Rudiana Agustini Rumanintya Lisaria Putri Ruslan L S.Pd. M Kes I Ketut Sudiana . Sahrullah Sahrullah Santosa Santosa Santri Chintia Purba sari hodijah Sartika Hutasuhut Seprianto, Seprianto Shaumi, Nurul Shinta M Trivena Shinta Maharani Trivena Sila Silalahi Siswa Setyahadi Sitti Chadijah Situmorang, Adrian Slamet Santoso Sophia Rahmah Saleha Subaer Subaer Sufaidah, Siti Nur Sugiyarto Sugiyarto Sugiyarto Sugiyarto Suharmanto Suharmanto Sulaiman Yusuf Sulaiman, Sulaiman Syafruddin Haris Syafruddin Nasution Syahdan, St Syahril Nedi Syaputry Lita Yanti Syarifah Rohaya Tantri Analisawati Sudarsono Teguh Stiyawan Tengku Muhammad Ghazali Thamrin Wikanta Titania Tjandrawati Nugroho Titik Sumarti Treesia Sujana Tri A. Hadi Tri Emrinelson TRI PRANADJI Tri Winarni Tri Yulistyawati Evelina Tri Yulistyawati Evelina Usman Usman Wahyuda Hidayat Whitea Yasmine Slamet Widantari, Meilya widyaningrum, Astri WULANDARI, APRILIA Y Hutasoit, Anjeli Yayi Suryo Prabandari Yuana Nurulita Yudi Kusnadi Yuni Fitria Yusak Maryunianta Yuslina Octavia Yusni Ikhwan Siregar Yusril Has Yusrizal Yusrizal YUYUN SRIKANDI Zainuri -, Zainuri Zebua, Abdi Hikmat Petra Zevira, Bella Permata Zhilalul Huda