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KEMAMPUAN LEMNA (LEMNA PERPUSILLA TORR.) SEBAGAI FITOREMEDIATOR UNTUK MENYERAP LIMBAH NITROGEN DALAM BUDIDAYA IKAN LELE (CLARIAS GARIEPINUS) DI SISTEM RESIRKULASI Amalia, Febrina; Nirmala, Kukuh; Harris, Enang; Widiyanto, Tri
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 2 (2014)
Publisher : Research Center for Limnology

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Abstract

Penelitian ini bertujuan untuk mengkaji kemampuan lemna (Lemna perpusilla Torr.) sebagai fitoremediator dalam menyerap limbah nitrogen dalam budidaya ikan lele (Clarias gariepinus). Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 3 ulangan. Perlakuan berupa luas tutupan lemna sebesar 14,7%, 29,4%, dan 44,1% dari kolam filter. Padat tebar ikan lele adalah 200 ekor m-3 dengan bobot rata-rata awal 9,67±1,01 g. Hasil penelitian setelah 30 hari menunjukkan bahwa perlakuan luas tutupan lemna 44,1% dapat menyerap limbah N sebesar 4,48±0,04 g N, sedangkan untuk perlakuan luas tutupan lemna 29,4% dan 14,7% dapat menyerap limbah N masing-masing sebesar 4,03±0,02 g N dan 3,50±0,07 g N. Jumlah N dalam biomassa lemna tertinggi juga dicapai oleh perlakuan luas tutupan lemna 44,1% sebesar 28,13±0,74 g N. Sintasan tertinggi 76,33±4,04% juga diperoleh pada perlakuan luas tutupan 44,1%.
PEMANFAATAN Lemna perpusilla SEBAGAI PAKAN KOMBINASI UNTUK IKAN NILA (Oreochromis niloticus) PADA SISTEM RESIRKULASI Ilyas, Anita Prihatini; Nirmala, Kukuh; Harris, Enang; Widiyanto, Tri
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 2 (2014)
Publisher : Research Center for Limnology

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Abstract

Lemna perpusilla adalah suatu makrofit yang hidup terapung di air, terdapat di seluruh dunia dan banyak ditemukan di air tawar yang kaya nutrien. Tumbuhan ini lebih dikenal sebagai gulma yang cenderung sulit untuk dikendalikan karena memiliki produktivitas yang sangat tinggi. Penelitian untuk menganalisis kemampuan ikan nila (Oreochromis niloticus) dalam memanfaatkan L. perpusilla sebagai pakan kombinasi telah dilakukan. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan empat taraf perlakuan dan tiga ulangan. Perlakuan konsentrasi pakan 100% L. perpusilla + 0% pelet, 25% L. perpusilla + 75% pelet, 50% L. perpusilla + 50% pelet, 0% L. perpusilla + 100% pelet. Ikan yang digunakan dalam penelitian ini adalah ikan nila (O. niloticus). Padat tebar ikan 20 individu per waring dengan bobot rata-rata 20±0,01 g per individu. Ikan diberi pakan sebanyak dua kali per hari selama 50 hari. Setiap tujuh hari sekali dilakukan penimbangan bobot tubuh ikan nila. Hasil penelitian menunjukkan bahwa L. perpusilla dapat menggantikan pelet sebagai pakan sebesar 25%. Lemna tidak dapat menggantikan pakan secara keseluruhan karena terkait dengan tingginya serat yang terkandung di dalamnya yang dapat mempersingkat waktu tubuh untuk melakukan proses pencernaan dan penyerapan nutrisi.
STUDI MENGENAI TOKSISITAS SURFAKTAN DETERJEN, ALKYL SULFATE (AS), TERHADAP POST LARVA UDANG WINDU Penaeus monodon Fabr Eddy Supriyono; Berlianti .; Kukuh Nirmala
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 15 No. 2 (2008): Desember 2008
Publisher : Institut Pertanian Bogor

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Abstract

Penelitian ini dilakukan untuk mengetahui toksisitas surfaktan deterjen alkyl sulfate (AS) terhadap post larva udang windu Penaeus monodon Fabr. Pada uji akut, udang windu PL10 dipaparkan pada media yang mengandung AS selama 96 jam dan dihitung nilai Median Lethal Concentration (LC50) pada jam ke 24, 48, 72, dan 96. Pada uji sub kronis udang windu PL15 dipaparkan selama 24 hari dan diamati nilai laju pertumbuhannya. Selama uji toksisitas akut dan sub-kronis juga dilakukan pengamatan perubahan histopatologi pada insang dan hepatopankreas udang. Hasil penelitian ini menunjukkan bahwa nilai LC50 AS pada jam ke- 24, 48, 72, dan 96 yaitu masing-masing sebesar 33.6, 29.4, 24.3, dan 22.8 mg/l. Saat uji sub kronis, perlakuan pemberian AS terhadap media pemeliharaan menurunkan laju pertumbuhan udang windu seiring dengan meningkatnya konsentrasi AS dan secara nyata terlihat mulai konsentrasi 17.11 mg/l. Selama uji akut dan sub kronis terjadi perubahan tingkah laku dan kerusakan pada insang dan hepatopankreas pada udang yang dipaparkan dengan AS mulai terlihat pada konsentrasi 25.58 mg/l jam ke-96 dan 34.99 mg/l jam ke- 72 pada uji akut dan pada uji sub kronis mulai terjadi pada konsentrasi 9.78 mg/l pada pengamatan hari ke- 24. Toksisitas AS terhadap juvenil udang windu semakin meningkat dengan semakin meningkatnya konsentrasi dan waktu pemaparan.Kata kunci: toksisitas, udang windu, alkyl sulfate, LC50, pertumbuhan, histopathologi. This study was done to find out the toxicity of surfactant detergent alkyl suphate (AS) on post larvae of black tiger shrimp. For acute toxicity test, the PL10 shrimps were exposed in seawater containing AS for 96 hours and Median Lethal Concentration (LC50) for 24, 48, 72, and 96 hours were estimated. For sub chronic test, juvenile shrimps at PL15 were exposed in sea water media containing AS for 24 days, and the growth rate of the shrimps were evaluated in order to determine the toxicity effect of AS to juvenile shrimp. During acute and sub-chronic test the histopathological changed of the gills and hepatopanchreas of the shrimp were also examined. This study resulted that LC50 of AS for 24, 48, 72, and 96 hours was estimated to be 33.6, 29.4, 24.3, and 22.8 mg/l of AS. At sub-chronic level, the growth rate of the shrimps was decreased by increasing concentration of AS and significantly affected at 17.11 mg/l of AS. During acute and sub-chronic test the behavior changing and gill epithelium and hepathopancreatic cell damage was common occurred in the shrimps when exposed in AS at 25.58 mg/l of AS for 96 hours and 34.99 mg/l of AS for 72 hours exposed time during acute test and also occurred starting from 9.78 mg/l of exposed concentration of AS for 24 days exposed time. The toxicity of AS to juvenile tiger black shrimp elevated by the increased of exposure time. Key words: toxicity, Alkyl Sulphate, black tiger shrimp, LC50, growth, histopathology.
Identifikasi Dinamika Spasial Penggunaan dan Tutupan Lahan di Kabupaten Indramayu: Identification of Spatial Dynamics of Land Use and Cover in Indramayu Regency Muhammad Firmawan; Widiatmaka; Kukuh Nirmala
Jurnal Ilmu Tanah dan Lingkungan Vol 23 No 2 (2021): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

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Abstract

The increase in population occurs in Indonesia every year. This results in a high demand for space. Indramayu Regency is one of the regencies with high dynamics of land use and cover, which is indicated by an increase in residential area of 14.8 ha year-1 and a decrease in rice fields by an average of 13.9 ha year-1. This study aimed to identify the types of land use and cover in order to become a reference for analyzing the spatial dynamics of land use and cover in Indramayu Regency. Spatial dynamics of land use and cover were identified through Landsat 7 and Sentinel-2A which were then analyzed for overall accuracy, Kappa accuracy, validation using Google Earth, and overlays to produce maps of land use and cover dynamics in 2005, 2011 and 2021. The results showed that there are 8 classes of land use and cover in Indramayu Regency, namely forest, open land, dry land agriculture, dry land agriculture, mixed dry land, rice fields, settlements, ponds, water bodies. Paddy fields have a dominant area along the observation point (>61%) which are spread the central part of Indramayu Regency. The percentage of residential area increased by 0.8% from 33,724 ha (16.2%) to 35,377 ha (17.0%) in the period 2005-2021. Meanwhile, the percentage of pond area increased by 0.5% from 23,221 ha (11.1%) to 24,163 ha (11.6%) during that period. The increase in population and economic level are some of the factors causing the dynamics of land change in Indramayu Regency.
THE ADDITION OF ZEOLITE, ACTIVATED CARBON, CLOVE OIL, AND DIFFERENT SALINITY FOR GLUCOSE RESPONSE AND OSMOTIC PRESSURE ON JUVENILE GIANT PRAWN IN CLOSED TRANSPORT SIMULATION SYSTEM WITH HIGH DENSITY . Humairani; Eddy Supriyono; Kukuh Nirmala
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.896 KB) | DOI: 10.29244/jitkt.v8i1.12749

Abstract

Long distance transportation of prawn juvenile usually use a closed system. Indonesian farmers often face  problem in this juvenile transportation such as a low survival rate due to shrimp stress and changes in water quality during transportation. This study was aimed to determine the effect of salt, cloves oil, zeolite, and activated carbon for maintaining water quality in 24 hours juvenile giant prawn simulation transportation at high density. The study was conducted at laboratory scale with a complete randomized design with 4 treatments and 3 replicates. For each treatments, it was added 4.67 µl/L clove oil, 20 g of zeolite, 10 g of activated carbon within 4 (four) different salt concentration i.e., 0 g/L (A), 4.7 g/L (B), 9.4 g/L(C), 14.1 g/L(D) and K (without salt, clove oil, zeolites and activated carbon). The results showed that the addition of 9.4 g/L of salt, 4.67 µl/L clove oil, 20 g of zeolite, and 10 g of activated carbon produced the best results with the highest survival rate by 88±2% at the end of simulation transportation and 82±2.83% after rearing, water quality and physiological respons of shrimp remains in good condition at the simulation transportation and at rearing. Keywords: juvenile giant prawn, salt, high density, physiological responses
TOTAL HEMOCYTE COUNT AND HEMOLYMPH GLUCOSE CONCENRATION RESPONSE OF SPINY LOBSTER Panulirus homarus ON RATIO OF SHELTER Suhaiba Djai; Eddy Supriyono; Kukuh Nirmala; Kukuh Adiyana
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 1 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.756 KB) | DOI: 10.29244/jitkt.v9i1.17923

Abstract

This research was conducted to assess the physiological response of the lobster Panulirus homarus for the ratio of the shelters. The method used completely randomized design with two replicates of each treatments with shelter ratio (A) 1 : 5, (B) 3 : 5, (C) 4 : 5, (D) 5 : 5. Weight average for 184 lobsters with the stocking density of 23 lobsters for each treatment was 32.64 ± 0.58 g. The experiment was conducted for 60 days. The lobster was fed with trash fish and acclimatized for 7 days before the experiment. Observations on the physiologycal of every 10 days. The physiological responses that observed were total hemocyte count (THC) and hemolymph glucose concentration. The results showed that 4:5 was the best lobster shelter ratio because it could reduce stress levels. This is indicated by the stable values of THC and hemolymph glucose level during the experiment and supported by the growth of 57.28 ± 0.15 g and survival rate of 91.31 ± 2.60%. Keywords: lobster, Panulirus homarus, ratio, shelter, THC, glucose
IMPACT OF NURSERY USING DIFFERENT PHYSICAL FILTRATION SYSTEM ON HEMOLYMPH GLUCOSE LEVEL AND SURVIVAL RATE OF SPINY LOBSTER JUVENILE Panulirus homarus Ega Aditya Prama; Eddy Supriyono; Kukuh Nirmala; Kukuh Adiyana
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.072 KB) | DOI: 10.29244/jitkt.v9i2.19291

Abstract

The problems in culture of early juvenile phase of spiny lobster Panulirus homarus is low survival rate of the seed. One of strategies to improve production is using filtration system during nursery of spiny lobster. The aim of this study to evaluate differentation of physical filtration system on responsse stress and survival rate of juvenile P. homarus. Experimental design of this study consisted of four treatments and two replications. The treatments were using flow through system without protein skimmer and filter (K), filtration system with protein skimmer (SK), filtration system with top filter (F) and filtration system with combinations of protein skimmer and top filter (SKF). P. homarus with initial weight 0.18+0.01 g were cultured for 60 days with density 250 lobster/tank. During maintenance, P. homarus were fed trash fish with feeding rate 20% of body weight. Parameters of glucose hemolymph were evaluated to determine stress response. Stress response was determined at day 0, 6, 20 and 60. Parameters of survival rate were determined at the end of experiment. The result showed that the treatment of filtration system with combination of protein skimmer and top filter (SKF) decreased stress responsse by decreasing glucose hemolymph of juvenile P. homarus during maintenance. Survival rate also higher (P<0.05) in treatment SKF (33.2%) than other treatments. It is concluded that SKF was the best physical filtration system for juvenile period of spiny lobster P. homarus  Keywords: filtration system, glucose hemolymph, spiny lobster, survival rate
PENAMBAHAN SHELTER UNTUK PENGENDALIAN RESPONS FISIOLOGI DAN PRODUKSI KEPITING BAKAU Scylla serrata PADA SISTEM RESIRKULASI AKUAKULTUR Yuni Puji Hastuti; Priyo Handoyo Wicaksono; Wildan Nurusallam; Siska Tridesianti; Yuli Siti Fatma; Kukuh Nirmala; Iman Rusmana; Ridwan Affandi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 12 No. 1 (2020): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (632.563 KB) | DOI: 10.29244/jitkt.v12i1.30753

Abstract

Ketersediaan shelter (tempat perlindungan) pada kepadatan spesifik diharapkan dapat meningkatkan produksi kepiting bakau (Scylla serrata) dalam sistem resirkulasi. Shelter sebagai salah satu faktor abiotik berperan penting dalam mengurangi kematian yang disebabkan oleh kanibalisme kepiting dan menekan tingkat stres biota. Sistem Resirkulasi Akuakultur (RAS) dengan bak kotak kultur berkapasitas 60 L yang didukung oleh shelter diperkirakan menghasilkan respons fisiologis dan pertumbuhan kepiting bakau yang paling baik. Penelitian ini bertujuan untuk mengevaluasi dampak penambahan shelter pada lingkungan dengan sistem resirkulasi terhadap respons fisiologis dan produksi kepiting bakau S. serrata dengan kepadatan 10 kepiting per satu bak kultur yang berisi 60 L air laut. Penelitian ini dilakukan dengan tiga perlakuan penambahan shelter, yaitu 2 shelter (S2), 4 shelter (S4), 6 shelter (S6), dan kontrol tanpa shelter (C). S6 adalah perlakuan terbaik dengan tingkat kelangsungan hidup 73,33±5,8%, laju pertumbuhan spesifik 0,886±0,014%, pertumbuhan lebar karapas 0,024±0,004 cm/hari, dan rasio konversi pakan terendah dibandingkan dengan perlakuan lain. Perlakuan S6 secara signifikan mempengaruhi jumlah hemosit total kepiting pada awal budidaya (P <0,05). Penambahan 6 shelter dapat mengoptimalkan pertumbuhan kepiting lumpur dengan kepadatan 10 kepiting dalam satu bak kotak kultur.
Water Quality and Sediment Profile in Shrimp Culture with Different Sediment Redox Potential and Stocking Densities Under Laboratory Condition Wiyoto Wiyoto; Sukenda Sukenda; Enang Harris; Kukuh Nirmala; Daniel Djokosetiyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 2 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.507 KB) | DOI: 10.14710/ik.ijms.21.2.65-76

Abstract

Sediment quality has been considered as one of the prime factors influencing the environment quality that support maximum shrimp production.The aim of the study was toevaluate the effects of sediment redox potential and shrimp stocking density on the profile of some sediment and water quality parameters. Two factors randomized factorial design was applied, with stocking density (60 and 120 shrimps.m-2) as the first variable and sediment redox potential (-65 mV, -108 mV and -206 mV) as the second variable. Some significant changes in TP, total Mn, and total S concentrations in the sediment were observed after the experimentation (P<0.05). Sediment redox potential significantly affected the dissolved oxygen, TAN, NO2, NO3, and H2S concentrations in the water. Whereas shrimp stocking density affected all water quality parameters except H2S concentration. Significant interactions between redox potential and stocking densities were observed in the nitrite and alkalinity concentrations. The significant effects of both shrimp density and redox potential on the sediment and water parameters in particular those that are known to directly affect the shrimp welfare (e.g. oxygen, ammonia, nitrite and H2S) indicate that these variables are of important aspects in shrimp pond management. Furthermore, the results clearly showed that -206mV redox potential significantly reduced the dissolved oxygen concentration in the sediment-water interface and increased the generation of H2S in water column. Thereby, this redox potential level is not advisable for shrimp culture system. Keywords: redox potential, stocking density.
Organic carbon source and C/N ratio affect inorganic nitrogen profile in the biofloc-based culture media of Pacific white shrimp (Litopenaeus vannamei) Muhammad Hanif Azhar; Eddy Supriyono; Kukuh Nirmala; Julie Ekasari
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 1 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.736 KB) | DOI: 10.14710/ik.ijms.21.1.23-28

Abstract

Organic carbon source and C/N ratio play an important role in aquaculture system with biofloc technology application. Nitrogen control by adding carbohydrates to the water to stimulate heterotrophic bacterial growth by converting nitrogen into bacterial biomass. The study investigated the effect molasses, tapioca, tapioca by product and rice bran as carbon sources in a biofloc media at three different C/N ratios i.e. 10, 15, and 20 on total ammonia reduction in biofloc media. Five liters of biofloc media in a conical tank was prepared for each replicate, which consisted of 500 mL of biofloc suspension collected from a shrimp culture unit with biofloc technology application and 4.5 L seawater. Pacific white shrimp culture was performed in 40L glass aquaria at a shrimp density of 30/aquarium. There was a significant interaction between carbon source and the C/N ratio applied (P<0.05). The use of molasses resulted in the highest reduction rate irrespective to the C/N ratio. Keywords: molasses, tapioca, tapioca by product, rice bran, biofloc, total ammonia nitrogen
Co-Authors . Rasmawan Adianto, Asep Agus Priyadi Agus Waluyo Ahmad Habibie, Ahmad Alexander Burhani Marda, Alexander Burhani Alimuddin Alimuddin Anang Hari Kristanto Ani Widiyati Ani Widiyati Ani Widiyati Ani Widiyati Anwar, Rifky Alwafi Any Widiyati Aras, Annisa Khairani Arfan Afandi Arif Faisal Siburian Arlina Ratnasari Asep Rachmat Pratama Asri, Yuliana Atma Jaya Salman Muin Aziz, Haikal Azril, Muh Berlianti . Berry Juliandi Bianingrum Bianingrum Cecep Kusmana Chandra Yudistira, Chandra Chrismadha, Tjandra D. Djokosetiyanto D. Djokosetyanto Daniel Djokosetiyanto Daniel Djokosetiyanto Daniel Djokosetiyanto DEDI JUSADI Dewi Puspaningsih Dinamella Wahjuningrum Dinar Tri Soelistyowati Dini Islama Dodi Hermawan E. M. Adiwilaga E. Yuniar Eddy Supriyono Enang Haris Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja, Enang Harris Erlania Erlania Erna Yuniarsih Ernik Yuliana Eva Prasetiyono Evi Susanti Farah Diana farida farida Farida Farida Febrina Amalia Fernando Jongguran Simanjuntak Gunawan, Bambang Kusmayadi Hamim Hamim Hamsiah, , Hamzah, Aris Sando Hanif Azhara, Muhammad Haris luthfi Harton Arfah Hastiadi Hasan Humairani, Humairani Hutapea, John Harianto I Nyoman Radiarta Idil Ardi Ilyas, Anita Prihatini Imam Taufik Iman Rusmana Iman Sari Lubis, Vina Imron Imron Imron Imron, Imron Irzal Effendi Izhar Amirul Haq Jannesa Nasmi John Harianto Hutapea John Harianto Hutapea Joni Haryadi Julie Ekasari Kukuh Adiyana Kukuh Adiyana Kuntari, Wahyu Budi Kurnia Faturochman, Kurnia Kurnia Faturrohman Lilis Nurjanah M. Faisol Riza Ghozali M. Zairin Junior Melati, Aulia Firda Mia Setiawati Mochamad Nurdin Moh. Burhanuddin Mahmud Muh Azril Muhamad Yamin Muhammad Amien H Muhammad Firmawan Muhammad Zairin Jr. Munawar Khalil Nafisyah, Eva Nasrul, Muit Novi Susianti Nur Bambang Priyo Utomo Nur Fauziyah Nuradzani, Daffa Nurhidayat Nurhidayat Nurul Taufiqu Rochman Nurul Taufiqu Taufiqu Rochman Prama, Ega Aditya Pras, Eva Prasetiyono Pratama, Asep Rachmat Priyo Handoyo Wicaksono Puji Hastuti, Yuni Purnamawati, , Puspaningsih, Dewi Qorie Astria R. Wulandari Rahmadi Azis, Rahmadi Retnosari Retnosari Revfvi Al Ghaney Rizal Riani Rahmawati RIDWAN AFFANDI Ridwan Affandi Rina Hesti Utami Riri Ezraneti Riri Ezraneti Ris Dewi Novita Riza Purbo Widiasto Rizkiya, Iffi Rizky Armansyah Robin Robin Robin, , Ruku Ratu Borut Ruspindo Syahputra Sabilu, Kadir Sabilu, Murni Saputra, Henry Kasmanhadi Satya, Awalina Setyo Pertiwi Sheny Permatasari Sri Nuryati Sri Nuryati Sri Wahyuni Firman Sudarto Sudarto Sugeng Budiharsono Sugiarti Suhaiba Djai Suharyanto Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sumi Lestari, Fajar Syarif Budiman T. Budiardi Tatag Budiardi Tri Heru Prihadi Tri Heru Prihadi Tri Heru Prihadi Tri Widiyanto Tridesianti, Siska Triheru Prihadi, Triheru Tyas Setioaji Vika Yuniar Wa Iba, Wa Iba Wahyu Wahyu Wardani, Sulistia WIDANARNI WIDANARNI Widiatmaka Widiatmaka widiatmaka Widiyati, Any Wijianto Wijianto Wijianto Wijianto Wildan Nurusallam Wildan Nurusallam, Wildan Wildan Nurussalam Wirantari, Ayu Puspa Wisriati Lasima Wiwin Ambarwulan Wiyoto Wiyoto Y. Hadiroseyani Yogi Himawan yogi Himawan Yosmaniar Yosmaniar Yosmaniar Yosmaniar Yuli Siti Fatma Yuli Siti Fatma Yuliana Asri Yuliana Asri Yuni Puji Hastuti Yuni Puji Hastuti Yuni Puji Hastuti