p-Index From 2021 - 2026
7.272
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Kodifikasia: Jurnal Penelitian Islam Jurnal Agroteknos Eksplorium : Buletin Pusat Pengembangan Bahan Galian Nuklir Jurnal Penelitian Pertanian Terapan Sinkron : Jurnal dan Penelitian Teknik Informatika International Journal of Artificial Intelligence Research Acta Aquatica: Aquatic Sciences Journal JMM (Jurnal Masyarakat Mandiri) Jurnal Agrotek Tropika Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Riset Inspirasi Manajemen dan Kewirausahaan Dialogia: Jurnal Studi Islam dan Sosial JURNAL KAJIAN TEKNIK MESIN International Journal of Financial, Accounting, and Management International Journal of Economics, Business and Accounting Research (IJEBAR) Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) Jurnal Ilmiah Manajemen Kesatuan Dinasti International Journal of Digital Business Management Jurnal Ekonomi Bisnis Manajemen Prima Jurnal Agrotropika BAKTI BANUA : JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Mandala Pengabdian Masyarakat Jurnal Manajemen Informatika Jayakarta Journal of Multidisciplinary Applied Natural Science Jurnal Pendidikan Teknik dan Vokasional Journal of Agriculture (JoA) Research Review: Jurnal Ilmiah Multidisiplin JPNM : Jurnal Pustaka Nusantara Multidisiplin Amal Ilmiah: Jurnal Pengabdian Kepada Masyarakat Jurnal Pendidikan Profesi Guru Journal of Islamic Education Students Jurnal Ilmu Tarbiyah dan Keguruan Hut Publication Business and Management Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Management Studies and Business Journal Asian Journal of Environmental Research ISLAMITSCH FAMILIERECHT JOURNAL International Journal of Technology and Education Research JURNAL REKAYASA KIMIA & LINGKUNGAN CJPP Jurnal Vokasi Keteknikan Jurnal Proteksi Agrikultura Jurnal Ilmiah Manajemen Profetik Servqual: Jurnal Ilmu Manajemen Eksplorium : Buletin Pusat Pengembangan Bahan Galian Nuklir Journal of The Physical Society of Indonesia
Claim Missing Document
Check
Articles

Found 2 Documents
Search

Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp. Darmawan, Akhmad; Maulidiyah; Megawati; Ariani, Novita; Aisya, Sitti; Sukirno; Randy, Ahmad; Primahana, Gian; Hendra, Medi; Nurdin, Muhammad
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.14

Abstract

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAcĀ 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability
Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp. Darmawan, Akhmad; Maulidiyah; Megawati; Ariani, Novita; Aisya, Sitti; Sukirno; Randy, Ahmad; Primahana, Gian; Hendra, Medi; Nurdin, Muhammad
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.14

Abstract

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAcĀ 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability
Co-Authors A.R., Rasmawati Abdul Haris Watoni Abdul Muhaimin, Abdul Abdul Rachman Abidin, Muhammad Aceng Haetami, Aceng Achmad Noerkhaerin Putra Adhika Junara Karunianto Afriliyanti, Rizki Agus M. Hariri Agus Salim, La Ode Ahmad Kamil Ahmad Randy Ahmad Zatnika Purwalaksana, Ahmad Zatnika Ahmad Zulfan, Ahmad Aisya, Sitti Akhmad Darmawan Al Farizy, Firnas Aldi Dermawan, Mukhlis Alfiannor, Alfiannor Alvianna, Stella Anisah Ansharullah ansharullah Arham, Zul Ariani, Novita Arief Budi Nugroho, Arief Arif Efendi A.S. Arwanto, Arwanto As'adi, Muhammad Asmawi Asnawi Asnawi Azmi, Khusnul Ulul Bambang Setiawan Bedy Purnama Bijang, Catherina M. Catur Nugroho, Tommy Kristian Cipta Ginting, Cipta Devina Hutabarat, Yessica Dewanti, Elsa Mayorita Diah Widiastuti, Diah Diana Diana Diana, Ulfa Djohan, M Widda Dodik Jatmika Eka Fitrianto, Eka Elsa Sely Rahmayani Ermawati Ermawati Erwansyah, Ferli Evizal, M.S., Dr. Ir. Rusdi Faishol, Faris Fanlia Prima Jaya Fatimah Zahro Fauziah Fauziah Fembriarti Erry Prasmatiwi Fredita, Silva Yumi Ginting , Cipta Hadi Suntoko Halim, Levin HALIMATUSSADIYAH -, HALIMATUSSADIYAH Handayani, Ridha Hariyanto, Nanang Hasanah Nur Hasriadi Mat Akin Helina, Selvi Hendri, Afrizal Heri Syaeful Hery Syaeful Husna Fii Karisma Jannah I GEDE SWIBAWA, I GEDE Ifa, Rista Putri Nur Imam Hamzah Imran Imran Indriani, Annisa Inrianti, Inrianti Irwan Irwan Ivayani, Ivayani Jahri, Muhammad Joko Prasetyo Kadir, La Ode Abd Kasiono, Roy Khaliq, Muh. Idham Khouruh, Umu Khusmayudi, Khusmayudi Kurnia Setiawan Widana Kurniawan Kurniawan Kus Hendarto, Kus Kusuma, Arvi Yuniar La Ode Santiaji Bande Liandari, Rizky Fatma Lita Norfiana M. Agphin Ramadhan M. Natsir Maindi, Andri Jahir Mat Akin, Hasriadi Maulidiyah MAULIDIYAH -, MAULIDIYAH Maulidiyah Maulidiyah Medi Hendra, Medi Megawati Melati Meulisa, Ade Irma Muh. Edihar Muhammad Darwis Meyandie Nasution Muhammad Thoyib Muhammad Yahya Muhammad Yahya Muhammad Zakir Muliadi Muliyadi Muliyadi Mustapa, Faizal Muzakkar, Muhammad Zakir Muzakki, M Harir Nasriadi, Nasriadi Ngadenin Ngadenin Niti Lestari Nohong Nohong, Nohong Nur Aeny, Titik Nur Aini, Yuliani Nur Hidayanti, Fitry Nur, Hasanah Nurhikmah Nurhikmah Nursaibah Prima Jaya, Fanlia Primahana, Gian Priyanto, Imansyah Putri, Pinkan Amanda Putri, Syifa Oktaviani Putri, Viola Augustia R, Halimatussaddiyah Rachmadina, Najma Fadya Ratih Titi Komalasari Ratna Ratna Refdinal Nazir Repi, Viktor Vekky Ronald Rismiati, Rismiati RR. Ella Evrita Hestiandari Rusdi Evizal Saddam, Muhamad Safitri, Annisa Saidah, Andi Salim, La Ode Agus Sampurnawati Sampurnawati Sanjaya, Purba Saputra, Adam Saragih, Delima Sartapa Sartapa, Sartapa Sembiring, Rinawati Shanty Komalasari, Shanty Siti Mulasih Slamet Sudarto, Slamet SRI RAHAYU Sri Suryani Sudi Pramono . Sufal Diansyah Sugiatno Sugiatno Suharji Suharji SUKIRNO Sumiyati Tuhuteru Suparman Suparman Suskandini Ratih Dirmawati Sutoyo Sutoyo Syafril Syafril Syamsuddinnor, Syamsuddinnor Taufik Hidayat THAMRIN AZIS Titien Agustina Titik Nur Aeny Tri Maryono Tundo, Tundo Tusiana Dewi Umiatin, Umiatin Vidya Selasdini Wahab, Abd Wahyu Ningsih, Eka Yarianto Sugeng Budi Susilo, Yarianto Sugeng Budi Yusep Purwana Muslih