Article Per Year (5 Year)
p-Index From 2020 - 2025
5.907
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences EDUKASIA Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Kodifikasia: Jurnal Penelitian Islam Jurnal Agroteknos Eksplorium : Buletin Pusat Pengembangan Bahan Galian Nuklir Jurnal Penelitian Pertanian Terapan Sinkron : Jurnal dan Penelitian Teknik Informatika International Journal of Artificial Intelligence Research Acta Aquatica: Aquatic Sciences Journal Jurnal Agrotek Tropika Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Riset Inspirasi Manajemen dan Kewirausahaan Dialogia: Jurnal Studi Islam dan Sosial JURNAL KAJIAN TEKNIK MESIN International Journal of Economics, Business and Accounting Research (IJEBAR) Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) Dinasti International Journal of Digital Business Management Jurnal Agrotropika BAKTI BANUA : JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Mandala Pengabdian Masyarakat Jurnal Manajemen Informatika Jayakarta Journal of Agriculture (JoA) Research Review: Jurnal Ilmiah Multidisiplin JPNM : Jurnal Pustaka Nusantara Multidisiplin Amal Ilmiah: Jurnal Pengabdian Kepada Masyarakat Jurnal Pendidikan Profesi Guru Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Asian Journal of Environmental Research ISLAMITSCH FAMILIERECHT JOURNAL International Journal of Technology and Education Research Economics Development Analysis Journal JURNAL REKAYASA KIMIA & LINGKUNGAN CJPP Jurnal Proteksi Agrikultura Jurnal Ilmiah Manajemen Profetik Servqual: Jurnal Ilmu Manajemen
Muhammad Nurdin
Jurusan Teknik Elektro, ITB, Bandung
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

Published : 48 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp. Darmawan, Akhmad; Maulidiyah; Megawati; Ariani, Novita; Aisya, Sitti; Sukirno; Randy, Ahmad; Primahana, Gian; Hendra, Medi; Nurdin, Muhammad
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.14

Abstract

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability
Cytotoxic of Usnic Acid Isolated from Ramalina sp.: Cytotoxic of Usnic Acid Isolated from Ramalina sp. Darmawan, Akhmad; Maulidiyah; Megawati; Ariani, Novita; Aisya, Sitti; Sukirno; Randy, Ahmad; Primahana, Gian; Hendra, Medi; Nurdin, Muhammad
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.14

Abstract

Ramalina sp. (Ramalinaceae) is a type of lichen known to contain many active secondary metabolite compounds that have the potential to be used as medicine or medicinal raw materials. One of the biological activities possessed by Ramalina sp. lichen is its anticancer activity. This research aims to isolate and identify active secondary metabolite compounds from the methanol extract of the Ramalina sp. lichen and to find out the cytotoxic activity of the isolated compound against MCF7 breast cancer cells. Compound 1 (usnic acid) was successfully isolated from fraction A. The isolation and purification process was carried out starting with a maceration process using acetone solvent, followed by silica gel column chromatography using a gradient solvent system consisting of n-hexane, n-hexane:EtOAc, EtOAc, EtOAc:MeOH, and MeOH with 5% increment of polarity to obtain 17 fractions (F-1 to F-17). From the 17 fractions obtained, fraction 3 (F-3) and fraction 4 (F-4) (eluted with n-hexane:EtOAc 30%), which had the same TLC profile, were combined and named as fraction A. Compound 1 (50 mg) is a yellow needle crystal that was formed in a bottle of fraction A, which was obtained after the process of combining fractions F-3 and F-4 and solvent evaporation process. The crystals were then separated and purified with CHCl3 and MeOH. Compound 1 was then characterized based on spectroscopic data. Various spectroscopic analysis data, including FTIR, 1D- and 2D-NMR, and LC-ESI-MS, show that Compound 1 is a dibenzofuran derivative compound with 18 carbons (3 from carbonyl groups (C=O) and 3 from methyl groups) and 2 hydroxyl (-OH). Cytotoxicity assay showed that at a low concentration of 18.75 ug/mL, Compound 1 caused a 67.06% decrease in MCF7 viability
Co-Authors Abdul Muhaimin, Abdul Abdul Rachman Aceng Haetami, Aceng Achmad Noerkhaerin Putra Agus M. Hariri Agus Salim, La Ode Ahmad Randy Aisya, Sitti Akhmad Darmawan Alfiannor, Alfiannor Alvianna, Stella Ansharullah ansharullah Arham, Zul Ariani, Novita Arief Budi Nugroho, Arief Arwanto, Arwanto Asnawi Asnawi Bambang Setiawan Bijang, Catherina M. Cipta Ginting, Cipta Devina Hutabarat, Yessica Dewanti, Elsa Mayorita Diana, Ulfa Djohan, M Widda Dodik Jatmika Eka Fitrianto, Eka Elsa Sely Rahmayani Eni Sumarminingsih Ermawati Ermawati Evizal, M.S., Dr. Ir. Rusdi Faishol, Faris Fauziah Fauziah Fembriarti Erry Prasmatiwi Fernandes, Adji Fredita, Silva Yumi Ginting , Cipta Halim, Levin HALIMATUSSADIYAH -, HALIMATUSSADIYAH Handayani, Ridha Hariyanto, Nanang Hasriadi Mat Akin Helina, Selvi Hendri, Afrizal Hery Syaeful I GEDE SWIBAWA, I GEDE Imran Imran Indriani, Annisa Inrianti, Inrianti Irwan Irwan Ivayani, Ivayani Jahri, Muhammad Jamika, Dodik Joko Prasetyo Kadir, La Ode Abd Kasiono, Roy Khouruh, Umu Khusmayudi, Khusmayudi Kurnia Setiawan Widana Kurniawan Kurniawan Kus Hendarto, Kus Kusuma, Arvi Yuniar La Ode Santiaji Bande Liandari, Rizky Fatma Lita Norfiana M. Natsir Maindi, Andri Jahir Mat Akin, Hasriadi Maulidiyah MAULIDIYAH -, MAULIDIYAH Maulidiyah Maulidiyah Medi Hendra, Medi Megawati Melati Meulisa, Ade Irma Muh. Edihar Muhammad Darwis Meyandie Nasution Muhammad Thoyib Muhammad Yahya Mustapa, Faizal Muzakkar, Muhammad Zakir Muzakki, M Harir Nasriadi, Nasriadi Ngadenin Ngadenin Nohong Nohong, Nohong Nur Aeny, Titik Nur Aini, Yuliani Nur, Hasanah Nurhikmah Nurhikmah Nursaibah Primahana, Gian Priyanto, Imansyah Putri, Syifa Oktaviani Putri, Viola Augustia R, Halimatussaddiyah Rachmadina, Najma Fadya Ratih Titi Komalasari Ratna Ratna Refdinal Nazir Rismiati, Rismiati RR. Ella Evrita Hestiandari Rusdi Evizal Saddam, Muhamad Safitri, Annisa Saidah, Andi Salim, La Ode Agus Saragih, Delima Sembiring, Rinawati Shanty Komalasari, Shanty Solimun SRI RAHAYU Sudi Pramono . Sufal Diansyah Sugiatno Sugiatno SUKIRNO Sumiyati Tuhuteru Suparman Suparman Suskandini Ratih Dirmawati Sutoyo Sutoyo Taufik Hidayat THAMRIN AZIS Titien Agustina Titik Nur Aeny Tri Maryono Tundo, Tundo Tusiana Dewi Ullah, Muhammad Vidya Selasdini Wahyu Ningsih, Eka Yusep Purwana Muslih Zakir Muzakkar, Muhammad