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All Journal Jurnal Veteriner Media Kedokteran Hewan Universa Medicina AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Bima Abdi: Jurnal Pengabdian Masyarakat Berkala Penelitian Hayati Indonesian Andrology and Biomedical Journal Jurnal Kedokteran Hewan Smart Dedication: Jurnal Pengabdian Masyarakat
Suherni Susilowati
Departemen Reproduksi Veteriner, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Surabaya 60115
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Journal : Jurnal Veteriner

 1 
THE EFFECT OF INSULIN LIKE GROWTH FACTOR – I COMPLEX TO CORELATION MALONDIALDEHIDE CONCENTRATE WITH INTAC MEMBRAN PLASMA OF SPERM Suherni Susilowati
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1580.884 KB)

Abstract

The aim of this research was study of Insulin Like Growth -I Complex to level of malondialdehide (MDA) and percentage of intact membrane plasm. Report of several species suggest that seminal plasma contains factors that may influence sperm viability. Seminal plasma was reported to be important for maintaining spermatozoa motility in bull and ram. For improving ram sperm viability and for increasing the resistance of boar spermatozoa to cold shock damage. IGF-I has been identified in the testis, where secreted by Leydig and Sertoli cells. Collection of semen by using artificial vagina from male goats and then characterized of motility and viability of spermatozoa. Identification of Insulin Like Growth Factor –I Complex were done by Native-PAGE 12% techniques, after several bands were identified and was done isolate of first band protein Insulin Like Growth Factor-I Complex. Insulin Like Growth Factor – I (IGF-I) Complex seminal plasma have molecular weight of 150,288 kDa, respectively. Sperm preparation for this research by centrifugation of semen with Bracket and Oliphan’s medium. These sperm preparation were characterized of motility, viability. Then after sperm preparation divided into three groups. The group I, added with Bracket and Oliphan’t medium, group II added with Bracket Oliphan’t and IGF – I Complex medium, group III added with IGF – I Complex medium and then were exposed 45 minutes for incubation and then percentage of MPU and level of MDA was evaluated. The result of this research showed that percentage of level MDA has significant different between group I,group II and group III, (p<0,05). The percentage of MPU sperm has significant different between groups I, group II and group III (p<0,05). The highest of percentage MPU was found in group III and the lower of level MDA was found in group III. Regresion test showed that negative correlation between level of MDA and percentage of MPU sperm.
Peningkatan Ekspresi Vascular Endothel Growth Factor dan Mitogen Activating Protein Kinase Plasenta Tikus yang Dipapar Carbon Black ((INCREASING EXPRESSION ON VASCULAR ENDOTHELIAL GROWTH FACTOR AND MITOGEN ACTIVATING PROTEIN KINASE IN PLACENTA RATS EXPOSE Viski Fitri Hendrawan; Widjiati Widjiati; Suherni Susilowati; Pudji Srianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.745 KB) | DOI: 10.19087/jveteriner.2017.18.1.59

Abstract

Carbon black is one of the components of particulate matter (PM), known as cytotoxic, causing inflammationin the respiratory and cardiovascular system, and able to pass through the placental barrier, which may affect the fetus. The effects caused by pollutants depend on the intensity and time length of the exposure.The objective of this study is to identify Vascular Endothelial Growth Factor (VEGF) and Mitogen Activating Protein Kinase (MAPK) as an indicator of maternal placental inflammation in pregnant rats (Rattus norvegicus) exposed to carbon black with immunohistochemical technique. This study was a laboratory experiment with three treatment groups and seven replications using pregnant female white rats (Rattus norvegicus). Pregnant female rats were exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 during pregnancy of day 6 up to day 11. The next phase was the observation and examination of the expressions of VEGF and MAPK by immunohistochemical staining toward placenta of the white rats exposed to carbon black. The result of the study showed an increase in the expression of VEGF in the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 per inhale during the mid-period (6-11) of pregnancy. In addition, there was also an increasein the expression of MAPK on the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3and 1064 mg/m3 per inhale during the mid-period (day 6-11) of pregnancy. In conclusion, the expression of VEGF and MAPK are increased along with the increased dosage of carbon black and age of gestation. ABSTRAK Carbon black merupakan salah satu komponen partikulat matter (PM) yang diketahui bersifat sitotoksik serta menyebabkan terjadinya inflamasi pada sistem pernapasan dan kardiovaskular serta dapat melewati barier plasenta fetus sehingga dapat memengaruhi fetus. Efek yang ditimbulkan oleh polutan tergantung dari besarnya paparan dan lama waktu paparan. Penelitian ini bertujuan untuk mengidentifikasi Vascular Endothel Growth Factor (VEGF) dan Mitogen Activating Protein Kinase (MAPK) sebagai indikator imflamasi pada plasenta maternal tikus (Rattus norvegicus) yang dipapar carbon black dengan teknik imunohistokimia. Penelitian ini dilakukan dengan tiga kelompok perlakuan dan tujuh kali ulangan yang menggunakan tikus betina bunting. Tikus betina bunting dipapar dengan carbon black dosis 523 mg/m3 dan 1064 mg/m3 selama kebuntingan hari ke-6 sampai 11. Tahap berikutnya adalah pengamatan dan pemeriksaan ekspresi VEGF dan MAPK dengan pewarnaan imunohistokimia terhadap plasenta tikus putih yang telah dipapar dengan carbon black. Hasil penelitian adalah terjadi peningkatan ekspresi VEGF pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan (6-11) kebuntingan. Selain itu terjadi peningkatan ekspresi MAPK pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan(6-11) kebuntingan. Simpulan penelitian ini adalah ekspresi VEGF dan MAPK meningkat seiring dengan peningkatan dosis carbon black dan umur kebuntingan.
Protein Kasar Plasma Seminalis Sapi Menurunkan Kejadian Nekrosis Spermatozoa Kambing yang Disimpan pada Suhu Dingin (BULL SEMINAL PLASMACRUDE PROTEIN DECREASING THE PERCENTAGE OF NECROSISIN GOATSPERMATOZOA THAT KEPT IN COLD TEMPARATURE) Suherni Susilowati; Hardijanto .; Indah Norma Triana
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.169 KB)

Abstract

The aim of this study was to determine the benefits of bullseminal plasma crude proteinin goatspermatozoa diluent to improve of frozen semen quality.This study consists of three treatments: (i)treatmentcontrol (P0,goat semen + diluents ; (ii) treatment I(PI, diluent + goat semen + bull seminal plasmacrudeprotein (1:1);and (iii)treatment II (PII, diluent + goat semen+ bull seminal plasma crude protein (1:2). Alltreatments were kept in arefrigerator (5oC) andobserved for necrosis of goatspermatozoa, daily. The highestspermatozoa necrosis was observed in the diluter without the bull seminal plasma crude protein eitheratday1, 2, 3, 4orday 5. The necrosis was statistically significant different (p<0,05) between P0, PI and PII.withthe lowest percentagenecrosis at PI. In conclusion, the addition of bull seminal plasma crude proteinin skim milk diluentwill decrease theincidenceof necrosis ingoat spermatozoa which were kept in coldtemperature.
Co-Authors Abdul Samik, Abdul Abuzahra, Mutasem Adiana Mutamsari Witaningrum Aditya Irawan Agustono, Bodhi Akintunde, Adeyinka Oye Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Ali Agus Ali Agus Anas Bad’rosana Prawindi Anugrah, Alvyan Lantang Ardina Sahra Miranda Arini, Mirza Asafaizh Aris Puji Nugroho Ayuti, Siti Rani Azura, Sarah Brahmantya, Chandra Budi Utomo BUDI UTOMO Candika, Yossy Imam Cesaria, Mayrena Ayu Chusniati, Sri Damayanti L, Tita Damayanti, Tita Dani Hesti Savitri Dani Hesti Savitri, Dani Hesti Dewi, Aprilia Kurnia Dian Ayu Permatasari Diba, Lucky DWI WIJAYANTI Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum Erma Safitri ERMA SAFITRI Estoepangestie, A.T.Soelih Fajar Ramadhan Subiyantoro Fania Selfiannisa Hana Eliyani Hani Plumeriastuti Hardijanto . Hardijanto, Hardijanto Hariadi, Mas'ud Hendarti, Gracia Angelina Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri IMAM MUSTOFA Immelda, Kicky Hanis Indah Norma Triana, Indah Norma iqbal dwi warsito Ira Sari Yudaniayanti Irvanto, Rudi Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Khoirunnisa, Itrah Koestanti S, Emy Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Mas'ud Hariadi Mega, Toafan Satrio Meles, Dewa Ketut Mirni Lamid Moh. Sofiul Anam Muhammad Ikmal Robih Mustofa Helmi Effendi Nawangwulan, Tantri Nove Hidajati Nusdianto N Nusdianto N, Nusdianto Nusdianto Triakoso Pitaloka, Dinda Galuh Poedji Hastutiek Portia Sumarsono, Portia Prastika, Zeni Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama Putra Banusiandana Pratama, Abrian Panggalih Indra Prayogo, Danu Pudji Srianto Purnawan, Ahmad Budi Puspita, Satya Alysa Cahya Putri, Cindy Ercha Aulia Qodri, Winda Nurul Lailatil Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Ratnaningtyas, Nurrohmah Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Riza Zainuddin Ahmad Rochmah Kurnijasanti ROHMAH, LAILATUL Rosyada, Zulfi Nur Amrina Sabdoningrum, Emy Koestanti Sabdoningrum, Emy Koestanty Salahuddin, Muhammad Soleh Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Shinta Putri Quraini Soeharsono Soeharsono Soepranianondo, Koesnoto Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Sunarso, Agus Suprihati , Endang Supriyadi Supriyadi Supriyadi Suryaningsih, Hastini Suzanita Utama Swari, Wahyu Retno Syaiful Rizal Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tyasmingsih, Wiwiek Utama, Dirga Januar Surya Viski Fitri Hendrawan Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Widjiati Widjiati Widjiati Widjiati, Widjiati Widya Paramita Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yunita, Maya Nurwartanti