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AKTIVITAS ANTIBAKTERI KULIT DAUN, EKSUDAT, DAN DAGING DAUN LIDAH BUAYA (Aloe vera (L.) Burm.f.) TERHADAP BAKTERI Staphylococcus epidermidis Sri Ria Ranti; Minda Sari Lubis; Haris Munandar Nasution; Rafita Yuniarti
FARMASAINKES: JURNAL FARMASI, SAINS, dan KESEHATAN Vol. 3 No. 1 (2023): FARMASAINKES: JURNAL FARMASI, SAINS dan KESEHATAN
Publisher : Fakultas Farmasi Universitas Muslim Nusantara Al Washliyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32696/farmasainkes.v3i1.2374

Abstract

Lidah buaya (Aloe vera (L.) Burm.f.) merupakan tanaman yang memiliki banyak manfaat karena semua bagian dari tanaman ini dapat dimanfaatkan baik untuk perawatan tubuh maupun untuk mengobati berbagai penyakit yang saat ini banyak digunakan sebagai antibakteri untuk melawan bakteri penyebab infeksi pada kulit salah satunya bakteri Staphylococcus epidermidis. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kulit daun, eksudat dan daging daun lidah buaya (Aloe vera (L.) Burm.f.) terhadap bakteri Staphylococcus epidermidis. Metode Penelitian ini adalah metode eksperimental. Uji aktivitas antibakteri dilakukan dengan metode difusi cakram yang meliputi penyiapan sampel, identifikasi bakteri, pembuatan larutan uji, dan uji aktivitas antibakteri kulit daun, eksudat, dan daging daun lidah buaya (Aloe vera (L.) Burm.f.) terhadap bakteri Staphylococcus epidermidis. Data yang diperoleh dianalisis secara deskriptif kualitatif berupa diameter zona hambat. Hasil penelitian menunjukkan bahwa kulit daun lidah buaya pada konsentrasi 6,25%, 12,5%, 25% dan 50% termasuk kategori resistant (lemah). Eksudat lidah buaya pada konsentrasi 6,25% tidak ada zona hambat, dan konsentrasi 12,5%, 25%, 50% termasuk kategori resistant (lemah), sedangkan daging daun lidah buaya konsentrasi 6,25%, 12,5%, 25% dan 50% termasuk kategori resistant (lemah). Dapat disimpulkan bahwa kulit daun, eksudat dan Daging daun lidah buaya memiliki aktivitas antibakteri terhadap bakteri Staphylococcus epidermidis.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN SEDINGIN (Kalanchoe pinnata (Lam) Pers) TERHADAP BAKTERI Staphylococcus aureus dan Salmonella typhimurium Nofa Lismandaria; D. Elysa Putri Mambang; Haris Munandar Nasution; Yayuk Putri Rahayu
FARMASAINKES: JURNAL FARMASI, SAINS, dan KESEHATAN Vol. 3 No. 1 (2023): FARMASAINKES: JURNAL FARMASI, SAINS dan KESEHATAN
Publisher : Fakultas Farmasi Universitas Muslim Nusantara Al Washliyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32696/farmasainkes.v3i1.2378

Abstract

Daun sedingin (Kalanchoe Pinnata (Lam) Per) merupakan tanaman obat yang sering digunakan oleh masyarakat di Sumatra, di Aceh. Daun sedingin memiliki khasiat sebagai antibakteri. Tujuan dari penelitian ini untuk mengetahui aktivitas antibakteri ekstrak etanol daun sedingin (Kalanchoe Pinnata (Lam) Per) terhadap bakteri Staphylococcus aureus dan Salmonella typhimurium. Penelitian ini dilakukan dengan metode eksperimental dengan meliputi pengumpulan sampel, pemeriksaaan makroskopik, pemeriksaan mikroskopik, pembuatan simplisia, pemeriksaan karakteristik, skrining fitokima, pembuatan ekstrak etanol, selanjutnya dilakukan pengujian aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Salmonella typhimurium dengan metode difusi agar. Hasil pemeriksaan karakteristik makroskopik memberikan hasil bentuk daun bersudut empat, permukaan luas, warna hijau, bau khas dan rasa agak pahit. Hasil pemeriksaan mikroskopik, ditemukan fragmen hablur kalsium oksalat bentuk prisma, perenkim dengan hablur kalsium oksalat, parenkim dengan berkas pembuluh, epidermis bawah dengan stomata, dan epidermis atas dengan stomata. Hasil karakteristik simplisia diperoleh kadar air 2%, kadar sari larut dalam air 59,9%, kadar sari larut dalam etanol 13,5%, kadar abu total 3,6%, dan kadar abu tidak larut asam 1,33%. Skrining fitokimia serbuk simplisia dan ekstrak menunjukkan adanya kandungan alkaloid, flavonoid, tanin, saponin, steroid/triterpenoid, dan glikosida. Hasil uji aktivitas antibakteri ekstrak etanol daun sedingin pada bakteri Staphylococcus aureus memiliki diameter zona hambat lebih besar pada konsentrasi 100% dan 75% dibandingkan pada kosentrasi 25% dan 50% yaitu 6,35, dan 7,35 mm. Salmonella typhimurium pada konsentrasi 100%, dan 75% memiliki zona hambat lebih besar dari pada konsentrasi 25, dan 50% yaitu 7,58 dan 14,19 mm. Hasil penelitian dapat disimpulkan bahwa ekstral etanol daun sedingin memberikan aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Salmonella typhimurium.
PENGARUH METODE EKSTRAKSI TERHADAP EFEK SITOTOKSISITAS DAUN JAMBLANG (Syzygium cumini L.) pada LARVA UDANG Artemia salina Leach DENGAN METODE BRINE SHRIMP LETHALITY TEST Leni Safriani; Muhammad Pandapotan Nasution; Haris Munandar Nasution; Yayuk Putri Rahayu
FARMASAINKES: JURNAL FARMASI, SAINS, dan KESEHATAN Vol. 3 No. 1 (2023): FARMASAINKES: JURNAL FARMASI, SAINS dan KESEHATAN
Publisher : Fakultas Farmasi Universitas Muslim Nusantara Al Washliyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32696/farmasainkes.v3i1.2381

Abstract

Daun Jamblang (Syzygium cumini L) adalah tumbuhan yang banyak di budidayakan oleh masyarakat dan mempunyai banyak khasiat salah satunya sebagai obat antikanker karena mengandung senyawa flavonoid, alkaloid, saponin, tanin, fenol, dan steroid. Penelitian ini bertujuan untuk mengetahui nilai LC50 dari pengaruh perbedaan ekstraksi secara dingin dan panas terhadap efek sitotoksisitas ekstrak daun jamblang menggunakan pelarut etanol 96% pada larva Artemia salina Leach. Pada penelitian ini dilakukan pengujian skrining fitokimia dan karakteristik daun jamblang. Pengujian sitotoksisitas ekstrak maserasi dan soxhletasi daun jamblang menggunakan metode Brine Shrimp Lethality Test dilakukan pada konsentrasi: 100 µg/mL, 200 µg/mL, 300 µg/mL, 400 µg/mL, 500 µg/mL, 600 µg/mL, 700 µg/mL, 800 µg/mL, 900 µg/mL, 1000 µg/mL. Hasil uji fitokimia terhadap ekstrak maserasi dan soxhletasi daun jamblang mengandung alkaloid, flavonoid, saponin, tanin, steroid dan glikosida. Hasil pengujian karakterisasi daun jamblang pada kadar air 6,66 %, kadar sari larut air 19,64 %, kadar sari larut etanol 24,67 %, kadar abu total 4,44 %, dan kadar abu tidak larut asam 0,66 %. Efek sitotoksisitas ekstrak daun jamblang terhadap Artemia salina Leach pada ekstrak maserasi memperoleh nilai LC50 152,2942 µg/mL sedangkan ekstrak soxhletasi memberikan nilai 60,3905 µg/mL dan termasuk kategori sangat toksik berpotensi sebagai antikanker, sehingga yang paling efektif menghasilkan hasil maksimal ialah cara ekstraksi soxhletasi.
Penetapan kadar flavonoid total ekstrak etanol, fraksi etil asetat dan n-heksan pada daun kakao (Theobroma cacao L.) dengan metode spektrofotometri uv-vis Zikra Maqfirah; Muhammad Amin Nasution; M. Pandapotan Nasution; Haris Munandar Nasution
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.43

Abstract

Cocoa (Theobroma cacao L.)  is a plant that is cultivated on plantations in Indonesia. One part of the cacao plant that has the potential to be developed as a traditional medicine is the cacao leaf because it contains secondary metabolites of flavonoids. Flavonoids have many properties, including acting as antioxidants, protecting cell structures, anti-inflammatories, preventing osteophoresis, and antibiotics. The purpose of this study is to determine the content of secondary metabolites and total flavonoid levels in ethanol extract, ethyl acetate fraction and n-hexane in cocoa leaves. In this study, the initial steps taken are sample collection, sample processing into simplicia, simplicia characterization test then preparation of cocoa leaf ethanol extract by maceration method using 70% ethanol solvent, followed by partitioning process with liquid-liquid extraction method to obtain ethyl acetate fraction and n-hexane and phytochemical screening is carried out. Determination of total flavonoid levels using the UV-vis spectrophotometric method at a wavelength of 437 nm with quercetin as a comparison.The results of this study showed that a cocoa leaf ethanol extract contains alkaloid compounds, flavonoids, tannins, saponins, steroids and glycosides, the ethyl acetate fraction contains alkaloid compounds, flavonoids, tannins, steroids and glycosides, while the n-hexan fraction contains alkaloid compounds, flavonoids and steroids. Total flavonoid levels in cocoa leaves from an ethanol extract amounted to 39.1422±0.0540 mg QE/g, ethyl acetate fraction 45.274±0.0629 mg QE/g and n-hexane fraction 21.4812±0.7048 mg QE/g. The highest flavonoid levels are obtained in the ethyl acetate fraction.
Penetapan Kadar Kafein Ekstrak Daun Teh Hijau Dan Putih Camellia Sinensis (L.) Dengan Kromatografi Cair Kinerja Tinggi Selfia Lestary; Muhammad Amin Nasution; Ridwanto Ridwanto; Haris Munandar Nasution
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.199

Abstract

Tea, a widely popular and frequently consumed beverage, is integral to daily routines. Despite the potential long-term risks associated with excessive caffeine consumption, such as hypertension, heart disease, and stroke, caffeine is a prominent choice among a diverse spectrum of individuals in society. The objective of this research endeavour is to discern the caffeine content present in extracts derived from green and white tea leaves by applying the high-performance liquid chromatography (HPLC) method. The research procedure encompasses a series of stages, encompassing the preparation of botanical materials, extraction employing ethanol solvent, characteristic analysis, phytochemical screening, and quantification of caffeine levels within the green and white tea leaf extracts utilising the HPLC technique. The extraction process of green and white tea leaves entails maceration utilising 96% ethanol, followed by concentration via a rotary evaporator. The determination of caffeine concentrations is executed using the high-performance liquid chromatography method. A phytochemical screening analysis of ethanol extracts derived from green and white tea leaves unveiled a myriad of chemical compound classes, including alkaloids, flavonoids, saponins, tannins, and steroids. The quantification of caffeine content within the samples is ascertained by calculating the area under the curve (AUC) on the chromatograms of green and white tea extracts. The quantification results for caffeine content in the green tea extract reveal a value approximately equivalent to 98.4278069571 mg/g. The white tea extract demonstrates a content value of approximately 136.131488681 mg/g.
Penetapan kadar flavonoid total ekstrak etanol daun bunga melati (Jasminum sambac (L.) Sol. ex Aiton) dengan spektrofotometri Uv-Vis Hastri Kholifah; M. Pandapotan Nasution; Anny Sartika Daulay; Haris Munandar Nasution
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.201

Abstract

Traditional medicine uses plants with a natural ingredient content as its raw material. The bioactive compounds found in plants are secondary metabolites such as alkaloids, flavonoids, tannins, glycosides, steroids, and saponins. One of the plants containing secondary metabolite compounds is jasmine. This study aims to determine the chemical compounds contained in the ethanol extract and the total flavonoid content of the ethanol extract of jasmine leaves using the UV-Vis spectrophotometric method. The stages of this research included processing plant simplicia, making ethanol extract, examining characteristics, screening phytochemicals, and determining the total flavonoid content of the ethanol extract of jasmine leaves using the UV-Vis spectrophotometric method. Extract jasmine flower leaves by maceration method using 96% ethanol solvent; the extract obtained was concentrated using a rotary evaporator; then the total flavonoid content was determined using the UV-Vis spectrophotometry method. The research shows that the ethanol extract of jasmine flower leaves (Jasminum sambac (L.) Sol. ex Aiton) contains several secondary metabolite compounds, such as flavonoids, glycosides, alkaloids, saponins, steroids, and tannins. A number of steps were taken to find out how many flavonoids were in the ethanol extract as a whole. These included finding the maximum wavelength of quercetin, the operational time, making a quercetin calibration curve, and using UV-Vis spectrophotometry to determine how many flavonoids were in the extract. The analysis results reveal that the total flavonoid content in the ethanol extract of jasmine flower leaves is approximately 40.10911 ± 0.5878 mg QE/g.
Analisis aktivitas antioksidan ekstrak etanol daun jeruk kuok (Citrus nobilis L.) dengan metode DPPH (1,1-Diphenyl-2-Picrylhydrazyl) Nurul Hasanah; Rafita Yuniart; Haris Munandar Nasution; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.204

Abstract

When the body is excessively exposed to free radicals, the need for antioxidants increases as the body lacks sufficient protection. Some secondary metabolites in plants that can act as antioxidants include flavonoids, alkaloids, and tannins. This research aims to identify secondary metabolite compounds present in the raw material and extract of Citrus nobilis L. (kuok orange) leaves and evaluate antioxidant activity by determining IC50 values. This study employs several methods, including analyzing crude characteristics, phytochemical screening, and antioxidant activity testing using the DPPH (1,1-Diphenyl-2-picrylhydrazyl) method with spectrophotometry UV-Visible. The characterization results of the natural material indicate a water content of 6.66%, a water-soluble compound content of 19.64%, an ethanol-soluble compound content of 24.67%, a total ash content of 4.44%, and an acid-insoluble ash content of 0.66%. These findings align with values documented in the existing literature on MMI. Phytochemical screening results reveal that the crude material and ethanol extract of Citrus nobilis L. (kuok orange) leaves contain various chemical compounds, including alkaloids, flavonoids, saponins, tannins, glycosides, and steroids. The IC50 value for the ethanol extract of Citrus nobilis L. leaves is approximately 40.4587 g/mL, categorizing it as very strong. This indicates that the ethanol extract of Kuok orange leaves possesses secondary metabolite compounds with a highly potent antioxidant capability.  
Deteksi cemaran Staphylococcus aureus pada ayam krispy lokal di sekitar salah satu universitas kota Medan Yayuk Putri Rahayu; D. Elysa Putri Mambang; Haris Munandar Nasution; Adelia Ramadani
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.222

Abstract

Chicken meat is more in demand by consumers because it is easy to process, can be accepted by all levels of society and has an economical price. Food poisoning can be caused by enterotoxin contamination caused by Staphyococcus aureus. Management of chicken meat that is not up to standard and unhygienic can affect human health. The purpose of this study was to identify the presence of pathogenic bacteria S. aureus in crispy fried chicken meat from roadside carts and local fast food at one of Medan city University and to analyze the contamination value of S. aureus bacteria whether it meets the Indonesian National Standard (SNI) < 1X102 CFU/ml. The research method is employed to detect the presence of S. aureus in samples using both Baird Parker Agar Base (BPA) and Egg Yolk media simultaneously. Quantification of S. aureus contamination is carried out through the application of the Total Plate Count (TPC) method. Findings from two samples of local fried chicken, namely samples A and B, indicate a positive result for the presence of S. aureus, with contamination levels exceeding the threshold of 1 x 102 CFU/ml. This leads to non-compliance with the established SNI standards. Conversely, in the case of local crispy fried chicken samples C and D, the results indicate a negative presence of the S. aureus pathogenic bacteria, with contamination levels not exceeding the threshold of 1 x 102 CFU/ml. Therefore, these results comply with the stipulated SNI standards.
Uji aktivitas antibakteri fraksi n-heksana dan etil asetat daun kecombrang (Etlingera elatior) terhadap Staphylococcus aureus dan Escherichia coli Alfi Wahyudi Nasution; Haris Munandar Nasution; Minda Sari Lubis; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.228

Abstract

Infectious diseases caused by microorganisms are diseases that are commonly found in society. The therapy used to treat infections today by administering antibiotics. However, many cases of bacterial resistance to antibiotics are caused by the irrational use of antibiotics, so is necessary to develop alternatives to antibiotics derived from plants. glycosides that can function as antimicrobials. This study aims to determine the activity of the n-hexane and ethyl acetate fractions from kecombrang leaves against Staphylococcus aureus and Escherichia coli. This research was conducted experimentally, the independent variables consisted of the ethanol extract of kecombrang leaves, n hexane and ethyl acetate fractions. The dependent variable consisted of simplicia characteristic test, kecombrang leaf phytochemical screening, antibacterial activity test of kecombrang leaf fraction against S.aureus and E.coli. Antibacterial test using n-hexane and ethyl acetate fractions made with concentrations of 10%, 30%, 50% and 70%. the positive control used the antibiotic chloramphenicol and the negative control used DMSO, and the method used was agar diffusion using paper discs.The results of the antibacterial test showed that kecombrang leaves had an inhibitory effect on Staphylococcus aureus and Escherichia coli bacteria. The inhibition power of the ethyl acetate fraction was stronger than n hexane. The strongest inhibition was found in the ethyl acetate fraction with a concentration of 30.50 and 70% against Staphylococcus aureus, namely 10.9 mm, 12.6 mm and 14.15 mm. whereas in Escherichia coli bacteria, namely 10.5 mm, 12.3 mm. and 13.9mm. and based on the CLSI inhibition zone category, 2020, the concentration fraction of 70% is in the intermediate category, concentrations of 50, 30, and 10% are in the resistant category. While the positive control is categorized as sensitive to both bacteria.  
Uji aktivitas antibakteri formulasi sediaan sabun padat transparan ekstrak daun belimbing wuluh (Averrhoa bilimbi L.) terhadap bakteri Cutibacterium acnes Siti Aisyah Jamil; Yayuk Putri Rahayu; Minda Sari Lubis; Haris Munandar Nasution
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.234

Abstract

Acne is a skin disease that begins with an increase in sebum production which is exacerbated by an attack by the Cutibacterium acnes bacteria. Currently, there are many anti-acne preparations, one of which is in the form of soap, but only a few contain natural herbs. One plant that has an active substance or agent that has the potential to prevent and treat acne leaves belimbing wuluh (Averrhoa bililmbi L.) with its antibacterial properties. Besides having the potential to prevent and treat acne, this plant is also very easy to find in Indonesia. This research was conducted to see whether starfruit leaf extract could be formulated as soap preparation and whether it had antibacterial properties against Cutibacterium acnes. The research method for fresh starfruit leaves was made into Simplicia and extracted using 96% ethanol, phytochemical screening was carried out on fresh starfruit leaves and extracts. Transparent solid soap formulation with belimbing wuluh leaf extract with concentrations of 2.5%, 5%, and 7.5%. Evaluation preparations included physical quality tests, pH tests, foam height and stability tests, skin moisture tests, irritation tests, Panelist preference tests, and antibacterial activity against Cutibacterium acnes. The results and conclusions show that fresh starfruit leaves and extracts contain compounds belonging to class alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids, and glycosides. Belimbing wuluh leaf extract can be formulated into transparent solid soap preparation, has a distinctive aroma extract, is homogeneous, pH 9-11, with spreadability 26-29mm. Antibacterial activity included the insensitive category at all concentrations. The panelist's preferred formula was preparation with a concentration of 2.5%
Co-Authors Adelia Ramadani Adjelie, Tiara Adyani Maulinda Ahmad Rasyid Ridho Alaina, Nadila Alfi Wahyudi Nasution Amaliana, Amaliana Ananda , Nadia Andjelie, Tiara Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Apmarja, Sarah Utami Aris Munandar Aryunda, Divia Asmarani, Asmarani Aulia Fitri, Aulia Aulia Sari Auza , Maharani Ayu , Tri Indah Pagar Azhar, Gustika Barutu, Liza Anisa Shevia Basaniah Basaniah Bilal, Mohd. BJ Sutrisna Cici Andriani Cindy Priscilia Cindy Widya Rosa Br Simarmata Cut Dian Mala Luthfia Cut Erika Maulydya D Elysa Putri Mambang D Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang Daeng Elysa Putri Mambang Daulay , Anny Sartika Delvi Amira Hutagaol Dikki Miswanda Dina Agustia Parlin Dina Suciati Saragih Dinda Sari Utami Dini Rahmadani Dwi Jati, Fatma Ellyza, Chella Emmy Wiriandini Ercha Minanda Fahmi Fahmi Farida Yani Fathur Rahman Fatimah, Cut fatur Rahman, fatur Febiyani, Rica Fithri Pulungan, Ainil Fitri Mulyani, Fitri Fitri, Nurul Fitri, Raissa Friyani, Lilah Gabena Indrayani Dalimunthe Gultom, Syarifah Elena Hafizha, Putri Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Supia Indah Hastri Kholifah Humaira, Yara Hutagalung , Septi Ratna Cempaka Hutagalung, Riris Br Hutagaol, Delvi Amira Huzeila Nisa Siregar Ihsan Fadhilah Ika Yeni Siahaan Ilmi Widya Sari Imelda Natasia Br S Meliala Indah Nasution Indah Triutami Harahap Indrayani, Gabena Junia Fitri Hayani Khofifah , Nur Kiki Rawitri Krisna Juniharta Napitupulu Leni Safriani Lestari, Sri Indah Linda, Rosa Lubis, Minda Sari lubis, muhammad fauzan Lubis, Nadya Frestika Luthfianida, Arifna M Pandapotan Nasution M Pandapotan Nasution M Pandapotan Nasution M. Alfaruqi M. Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution Mambang , D.Elysa Putri Mambang, D. Elsya Putri Mambang, Elysa Putri Maya Syafira Mayani, Nurhikma Meutia, Cut Miftahul Jannah Minda Sar Lubis Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Ari Mukhtizar Muhammad Hizbullah Muhammad Pandapotan Nasution Muhammad Wahyu Ikhsan, Muhammad Wahyu Ikhsan Munaziatul Jannah Munthe, Herlina Nasri Nasri, Nasri Nasution , Muhammad Amin Nasution, Kartika Zsaskia Nasution, M. Pandapotan Nasution, Nur Sahadah Nasution, Wardah Natasya, Siti Adinda Nazirah, Nazirah Nazuhra, Oca Nelsa Fahira Ningtias, Anggitha Niswa, Khairun Nofa Lismandaria Novika Utami Nur Hanifah Nuraida Nuraida Nuratika, Eli Nurliansyah, Nurliansyah Nursyafira, Arini Nurul Hasanah Ovalina Sylvia Br. Ginting Pandapotan Nasution, M. Pertiwi, Rahma Prasetyo , Healthy Aldriany Purba , Intan Sabila Putri Puteri, Cut Intan Annisa Putri , Adilla Putri Intan Sari Putri Khairani Putri Mambang, D. Elysa Putri Rahayu, Yayuk Putri, Nadya Iwani Rafika, Intan Maya Rafita Yuniari Rafita Yuniart Rafliza , Rista Rahayu , Yayuk Putri Rahmadani , Adevika Rapita Yuniarti Rati , Yuliandini Rawitri, Kiki Ridho, Ahmad Rasyid Ridwanto Ridwanto Ridwanto Ridwanto Ridwanto Rifqi, M. Ainur Rina Azhari Ritonga , Septi Ani Riza Syafira Rizki, Ayu Fadillah RR. Ella Evrita Hestiandari Rukmana, Siti Sabila , Anisa Safira , Liyuza Safitri , Nurhaliza sahrianti, Hikmah Sari, Syilvi Rinda Sarmadansyah Sarmadansyah Sartika Daulay, Anny Sasnita, Merida Satri Situmorang, Rati Selfia Lestary Sepriani, Ananda Sinaga, Novita Yulianti Siregar , Anisah Siti Aisyah Jamil Situmorang, Lusi Grasia Situmorang, Rati Satri Sony , Suyefri Sri Harti Sri Natalia Saragih Sri Ria Ranti Sri Wahyuni Sri Wahyuni Sri Wahyuni Suci, Nurul Sulwiyatul K Sani Supiyani, Supiyani Syafira, Nona Syaiful Bahri Syalsabila Putri Syara, Nurdiani Syarifah Elena Gultom Tawir Rina Rezeki Tri Damaiyanti Trizaldi, Asep Try Santi Kisria Darsih Ummi Khairani Rambe Vera Estefania Kaban Wahdaniati , Tri Widya Sari Widya Sari Widya, Rizka Yanti, Rahma Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yuli Apriani Br.Lubis Yuniarti , Rafita Yuniarti, Rafita Zannah , Muhamad Zikra Maqfirah Ziza Putri Aisyia Fauzi Zulmai Rani