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Analisis aktivitas antioksidan ekstrak etanol daun jeruk kuok (Citrus nobilis L.) dengan metode DPPH (1,1-Diphenyl-2-Picrylhydrazyl) Nurul Hasanah; Rafita Yuniart; Haris Munandar Nasution; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.204

Abstract

When the body is excessively exposed to free radicals, the need for antioxidants increases as the body lacks sufficient protection. Some secondary metabolites in plants that can act as antioxidants include flavonoids, alkaloids, and tannins. This research aims to identify secondary metabolite compounds present in the raw material and extract of Citrus nobilis L. (kuok orange) leaves and evaluate antioxidant activity by determining IC50 values. This study employs several methods, including analyzing crude characteristics, phytochemical screening, and antioxidant activity testing using the DPPH (1,1-Diphenyl-2-picrylhydrazyl) method with spectrophotometry UV-Visible. The characterization results of the natural material indicate a water content of 6.66%, a water-soluble compound content of 19.64%, an ethanol-soluble compound content of 24.67%, a total ash content of 4.44%, and an acid-insoluble ash content of 0.66%. These findings align with values documented in the existing literature on MMI. Phytochemical screening results reveal that the crude material and ethanol extract of Citrus nobilis L. (kuok orange) leaves contain various chemical compounds, including alkaloids, flavonoids, saponins, tannins, glycosides, and steroids. The IC50 value for the ethanol extract of Citrus nobilis L. leaves is approximately 40.4587 g/mL, categorizing it as very strong. This indicates that the ethanol extract of Kuok orange leaves possesses secondary metabolite compounds with a highly potent antioxidant capability.  
Deteksi cemaran Staphylococcus aureus pada ayam krispy lokal di sekitar salah satu universitas kota Medan Yayuk Putri Rahayu; D. Elysa Putri Mambang; Haris Munandar Nasution; Adelia Ramadani
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.222

Abstract

Chicken meat is more in demand by consumers because it is easy to process, can be accepted by all levels of society and has an economical price. Food poisoning can be caused by enterotoxin contamination caused by Staphyococcus aureus. Management of chicken meat that is not up to standard and unhygienic can affect human health. The purpose of this study was to identify the presence of pathogenic bacteria S. aureus in crispy fried chicken meat from roadside carts and local fast food at one of Medan city University and to analyze the contamination value of S. aureus bacteria whether it meets the Indonesian National Standard (SNI) < 1X102 CFU/ml. The research method is employed to detect the presence of S. aureus in samples using both Baird Parker Agar Base (BPA) and Egg Yolk media simultaneously. Quantification of S. aureus contamination is carried out through the application of the Total Plate Count (TPC) method. Findings from two samples of local fried chicken, namely samples A and B, indicate a positive result for the presence of S. aureus, with contamination levels exceeding the threshold of 1 x 102 CFU/ml. This leads to non-compliance with the established SNI standards. Conversely, in the case of local crispy fried chicken samples C and D, the results indicate a negative presence of the S. aureus pathogenic bacteria, with contamination levels not exceeding the threshold of 1 x 102 CFU/ml. Therefore, these results comply with the stipulated SNI standards.
Uji aktivitas antibakteri fraksi n-heksana dan etil asetat daun kecombrang (Etlingera elatior) terhadap Staphylococcus aureus dan Escherichia coli Alfi Wahyudi Nasution; Haris Munandar Nasution; Minda Sari Lubis; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.228

Abstract

Infectious diseases caused by microorganisms are diseases that are commonly found in society. The therapy used to treat infections today by administering antibiotics. However, many cases of bacterial resistance to antibiotics are caused by the irrational use of antibiotics, so is necessary to develop alternatives to antibiotics derived from plants. glycosides that can function as antimicrobials. This study aims to determine the activity of the n-hexane and ethyl acetate fractions from kecombrang leaves against Staphylococcus aureus and Escherichia coli. This research was conducted experimentally, the independent variables consisted of the ethanol extract of kecombrang leaves, n hexane and ethyl acetate fractions. The dependent variable consisted of simplicia characteristic test, kecombrang leaf phytochemical screening, antibacterial activity test of kecombrang leaf fraction against S.aureus and E.coli. Antibacterial test using n-hexane and ethyl acetate fractions made with concentrations of 10%, 30%, 50% and 70%. the positive control used the antibiotic chloramphenicol and the negative control used DMSO, and the method used was agar diffusion using paper discs.The results of the antibacterial test showed that kecombrang leaves had an inhibitory effect on Staphylococcus aureus and Escherichia coli bacteria. The inhibition power of the ethyl acetate fraction was stronger than n hexane. The strongest inhibition was found in the ethyl acetate fraction with a concentration of 30.50 and 70% against Staphylococcus aureus, namely 10.9 mm, 12.6 mm and 14.15 mm. whereas in Escherichia coli bacteria, namely 10.5 mm, 12.3 mm. and 13.9mm. and based on the CLSI inhibition zone category, 2020, the concentration fraction of 70% is in the intermediate category, concentrations of 50, 30, and 10% are in the resistant category. While the positive control is categorized as sensitive to both bacteria.  
Uji aktivitas antibakteri formulasi sediaan sabun padat transparan ekstrak daun belimbing wuluh (Averrhoa bilimbi L.) terhadap bakteri Cutibacterium acnes Siti Aisyah Jamil; Yayuk Putri Rahayu; Minda Sari Lubis; Haris Munandar Nasution
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.234

Abstract

Acne is a skin disease that begins with an increase in sebum production which is exacerbated by an attack by the Cutibacterium acnes bacteria. Currently, there are many anti-acne preparations, one of which is in the form of soap, but only a few contain natural herbs. One plant that has an active substance or agent that has the potential to prevent and treat acne leaves belimbing wuluh (Averrhoa bililmbi L.) with its antibacterial properties. Besides having the potential to prevent and treat acne, this plant is also very easy to find in Indonesia. This research was conducted to see whether starfruit leaf extract could be formulated as soap preparation and whether it had antibacterial properties against Cutibacterium acnes. The research method for fresh starfruit leaves was made into Simplicia and extracted using 96% ethanol, phytochemical screening was carried out on fresh starfruit leaves and extracts. Transparent solid soap formulation with belimbing wuluh leaf extract with concentrations of 2.5%, 5%, and 7.5%. Evaluation preparations included physical quality tests, pH tests, foam height and stability tests, skin moisture tests, irritation tests, Panelist preference tests, and antibacterial activity against Cutibacterium acnes. The results and conclusions show that fresh starfruit leaves and extracts contain compounds belonging to class alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids, and glycosides. Belimbing wuluh leaf extract can be formulated into transparent solid soap preparation, has a distinctive aroma extract, is homogeneous, pH 9-11, with spreadability 26-29mm. Antibacterial activity included the insensitive category at all concentrations. The panelist's preferred formula was preparation with a concentration of 2.5%
Penetapan Kadar Kafein Dari Ekstrak Daun Kopi Robusta (Coffea canephora Pierre ex A. Froehner) Dan Daun Kopi Arabika (Coffea arabica L.) Dengan Metode Kromatografi Cair Kinerja Tinggi. Tri Damaiyanti; Muhammad Amin Nasution; Haris Munandar Nasution; Rafita Yuniarti
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.257

Abstract

Coffee is a plant that contains caffeine and can be processed into a delicious drink. Currently, it has achieved very high popularity throughout the world, ranking second after water and tea in the list of most preferred drinks. Coffee drinks are popular with various groups, from teenagers to adults, and in Indonesia, coffee is even the most giant drink consumed, only behind water in consumption levels. On average, people consume coffee around 3-4 times a day, reflecting how popular this drink is among the Indonesian population. Coffee has relatively high levels of caffeine, and continued excessive consumption can increase the risk of developing several types of diseases, such as hypertension, heart disease, and stroke. According to the Food and Drug Monitoring Agency (BPOM), the maximum limit for caffeine consumption in food and drinks is 150 mg per day and 50 mg per serving. Excessive and continuous coffee consumption can increase the risk of developing certain diseases such as hypertension, heart disease and stroke. This research aimed to determine the secondary metabolite content of robusta coffee leaves and arabica coffee leaves and to determine the caffeine content in robusta coffee leaves and arabica coffee leaves using high-performance liquid chromatography. The stages of this research include processing plant material, characterisation, making ethanol extract, phytochemical screening, and determining caffeine content in robusta coffee leaf extract and Arabica coffee leaves using high-performance liquid chromatography. Extracts from Robusta coffee leaves and Arabica coffee leaves are made using the maceration method using 96% ethanol. The resulting extract was then concentrated using a rotary evaporator, and qualitative testing of caffeine was carried out using the Parry method. Caffeine levels were determined using high-performance liquid chromatography, using the regression equation y = ax+b, where y is the area, a= 49.668, and b= - 16.536. The caffeine content in Robusta coffee leaf extract was measured at around 51.42916 mg/g, while Arabica coffee leaf extract had a caffeine content of around 29.97927 mg/g.
Uji Aktivitas Antibakteri Fraksi N-Heksan Kulit Bawang Merah (Allium cepa L.) Terhadap Bakteri Streptococcus mutans Ika Yeni Siahaan; Haris Munandar Nasution; Muhammad Amin Nasution; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.258

Abstract

Caries, or cavities, is a disease caused by damage to the enamel layer caused by the activity of bacteria in the mouth, one of which is the Streptococcus mutans bacterium. One of the plants that is useful as an antibacterial is the red onion (Allium cepa L.). The objective of this research was to determine the content of secondary metabolites in shallot skin and the antibacterial activity of the n-hexane and ethyl acetate fractions of shallot skin against Streptococcus mutansbacteria. An antibacterial activity test was carried out using the Kirby-Bauer diffusion method using paper discs. Empty Kirby-Baurer disks were dipped for 15 minutes into each solution of the n-hexane fraction and ethyl acetate fraction of shallot skin in various concentrations of 10%, 30%, 50%, and 70%. The positive controls used were amoxicillin disks, and the negative controls used DMSO. The content of secondary metabolites in the ethyl acetate fraction of shallot skins, which are semi-polar, is alkaloids, flavanoids, and tannins. Meanwhile, the n-hexane fraction of shallot skin contains nonpolar compounds, namely saponins and steroids or triterpenoids. The antibacterial test results of the ethyl acetate fraction of shallot skin obtained the diameter of inhibition at a concentration of 10% (7.33 ± 1.52), a concentration of 30% (9.67 ± 1.52), a concentration of 50% (13.33 ± 1, 52), and a 70% concentration (15.67 ± 1.52). While the n-hexane fraction of shallot skin obtained the diameter of the inhibition zone at a concentration of 10% (1.00 ± 1.00), a concentration of 30% (8.67 ± 1.52), a concentration of 50% (9.67 ± 1.52), and a 70% concentration (11.33 ± 1.52). The n-hexane fraction and the ethyl acetate fraction of shallot skin (Allium cepa L.) had antibacterial activity against Streptococcus mutans, with the best inhibition being the ethyl acetate fraction at a concentration of 70% classified as intermediate.
Skrining fitokimia dan uji aktivitas antibakteri ekstrak etanol daun kayu Jawa ((Lannea coromandelica) (Houtt) Merr terhadap bakteri Propionibacterium acnes dan Staphylococcus epidermidis Munaziatul Jannah; Haris Munandar Nasution; M. Pandapotan Nasution; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.241

Abstract

Jawa wood (Lannea coromandelica) (Houtt.) Merr. is a tropical plant that has potential as a medicinal plant. Almost all parts of this plant can be used in traditional medicine. This wild plant can be found easily in various locations such as home gardens, roadsides and community gardens. This study aims to evaluate the potential antibacterial activity of ethanol extract from the leaves of Kayu Jawa (Lannea coromandelica) (Houtt.) Merr. Against the growth of Propionibacterium acnes and Staphylococcus epidermidis bacteria. This research used experimental methods involving sample collection, macroscopic examination, microscopic examination, simplicia making, characteristic examination, Phytochemical Screening, and Antibacterial Activity Test using ethanol extract from Kayu Jawa ((Lannea coromandelica) (Houtt) Merr) against the bacteria Propionibacterium acnes and Staphylococcus epidermidis, using the agar diffusion method. Macroscopic examination results show that the leaves of Kayu Jawa have a tapered shape with a total of 7-11 strands. The flowers produced are single and yellowish-green in color, while the fruit has seeds of a certain length. Microscopic examination reveals the upper epidermis, palisade fragments, coral flowers, and wooden vessels with thickened spirals and stairs. The characteristics of simplicia show a water content of 4%, soluble essence content in water 21%, soluble essence content in ethanol 23%, total ash content of 4.8%, and insoluble ash content of 0.4% acid. The results of phytochemical screening on simplicia powder and ethanol extract of Kayu Jawa leaves showed contents such as alkaloids, tannins, flavonoids, steroids/triterpenoids, saponins and glycosides. The antibacterial activity test showed that Jawa wood showed potential as an antibacterial agent, with different inhibitory abilities depending on the concentration used. At a concentration of 20%, an inhibition zone of 6.85 mm was observed against Propionibacterium acnes and 5.75 mm against Staphylococcus epidermidis. Meanwhile, at a concentration of 80%, the area of inhibition increased to 14.15 mm against Propionibacterium acnes and 11.75 mm against Staphylococcus epidermidis.
Uji aktivitas antibakteri ekstrak etanol asam sunti terhadap bakteri Streptococcus mutans Novika Utami; Haris Munandar Nasution; Rafita Yuniari; Yayuk Putri Rahayu
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.286

Abstract

Asam sunti is belimbing wuluh, which is processed into a special food additive from Aceh Province because it becomes dregs and interferes. Belimbing is processed into asam sunti for seasoning or additional dishes. Belimbing contains active compounds such as alkaloids, triterpenoids, saponins, tannins, and flavonoids. Because Belimbing has an antibacterial pharmacological effect, it has been shown to fight bacteria that cause dental caries. The main cause of dental caries is the activity of Streptococcus mutans bacteria. The researcher took the research problem regarding the test of the inhibitory power of asam sunti extract. They know the antibacterial activity of 96% ethanol extract. The method used in this study was an experimental method with independent variables, namely asam sunti simplicia and asam sunti concentration. The main variables were the secondary characteristics and the metabolite content of the asam sunti ethanol extract. The tests included inhibiting asam sunti's ethanol extract against bacteria. The results of plant identification showed that the antibacterial inhibition zone of the ethanol extract of asam sunti belimbing wuluh ethanol extract with a concentration of 20% (18.54) mm, 40% (20.79) mm, and concentration of 60% (21.48) mm including Thus is known that the concentration of ethanol extract of asam sunti Belimbing wuluh on 20%-60% is an effective concentration to inhibit Streptococcus mutans bacteria The concentration of the ethanol extract has very strong antibacterial powder, causing a large inhibition zone.
Skrining fitokimia dan isolasi senyawa flavonoid dari ekstrak etanol kulit buah menteng (Baccaurea racemosa (Reinw.) Müll.Arg) Muhammad Ari Mukhtizar; Haris Munandar Nasution; M. Pandapotan Nasution; Rafita Yuniarti
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.299

Abstract

The menteng plant, which belongs to the Euphorbiaceae family, is a subtropical plant worldwide. Flavonoids are one of the groups of phenolic compounds found in plant tissues that can act as antioxidants. This research aimed to determine the class of chemical compounds found in the Menteng fruit peel and determine the characteristics of fruit peel flavonoids by UV Spectrophotometry and IR Spectrophotometry. %. Phytochemical screening showed positive for alkaloids, flavonoids, and steroids/triterpenoids, while tannins, saponins, and glycosides were negative from 400 grams of simplisia produced 39.1 grams of viscous extract. Characterization of crystal isolates of UV spectrophotometry showed a wavelength of 280 nm, inferred to be a flavanoid. The results of IR spectrophotometry show the O-H, C-H aliphatic, C=C aromatic, C=O, C-H, and C-O groups.
Penetapan kadar fenolik total ekstrak kayu bajakah (Spatholobus littolaris Hassk.) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri Uv-Vis Indah Triutami Harahap; Anny Sartika Daulay; Fathur Rahman; Haris Munandar Nasution
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.302

Abstract

The root of the Bajakah tampala (Spatholobus littolaris Hassk.) is one of the plants that is empirically used by the people of the interior of Central Kalimantan as a traditional medicine. According to the preliminary tests conducted, Bajakah Tampala contains phenolics. Phenolic compounds are compounds that have antioxidant activity. The objective of this research was to determine the class of compounds contained in the macerated extract and the total phenolic content and yield of the macerated extract in Bajakah roots. The stages of this research included the processing of plant materials, preparation of macerated and pirated root extracts, characterization examination, phytochemical screening, and the effect on the yield of total phenolic content of Bajakah roots using the UV-Vis spectrophotometry method. The results showed that the simplicia powder of the root of the Bajakah wood contained alkaloids, glycosides, steroids and triterpenoids, flavonoids, phenolics, saponins, and tannins, while the ethanol extract of the roots of the Bajakah wood showed the presence of alkaloids, steroids and triterpenoids, tannins, saponins, flavonoids, and phenolic compounds. The determination of total phenolic content was carried out by determining the maximum wavelength of gallic acid and calculating the total phenolic content based on the difference in ethanol concentration using the UV-Vis spectrophotometry method. The results for determining total phenolic content in 96% ethanol extract were 33.872 ± 0.0420 mg GAE/g, 70% ethanol extract was 29.345 ± 0.2149 mg GAE/g, and 50% ethanol extract was 18.512 ± 0.1355 mg GAE/g. So it can be concluded that the total phenolic content of 96% ethanol extract is higher than that of 70% ethanol extract and 50% ethanol extract.
Co-Authors Adelia Ramadani Adjelie, Tiara Adyani Maulinda Ahmad Rasyid Ridho Alaina, Nadila Alfi Wahyudi Nasution Amaliana, Amaliana Ananda , Nadia Andjelie, Tiara Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Anny Sartika Daulay Apmarja, Sarah Utami Aris Munandar Aryunda, Divia Asep Trizaldi Asmarani, Asmarani Aulia Fitri, Aulia Aulia Sari Auza , Maharani Ayu , Tri Indah Pagar Azhar, Gustika Barutu, Liza Anisa Shevia Basaniah Basaniah BJ Sutrisna Cici Andriani Cindy Priscilia Cindy Widya Rosa Br Simarmata Cut Dian Mala Luthfia Cut Erika Maulydya D Elysa Putri Mambang D Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang D. Elysa Putri Mambang Daeng Elysa Putri Mambang Daulay , Anny Sartika Delvi Amira Hutagaol Dikki Miswanda Dina Agustia Parlin Dina Suciati Saragih Dinda Sari Utami Dini Rahmadani Ellyza, Chella Emmy Wiriandini Ercha Minanda Farida Yani Fathur Rahman Fatimah, Cut Fatma Dwi Jati fatur Rahman, fatur Febiyani, Rica Fithri Pulungan, Ainil Fitri Mulyani, Fitri Fitri, Nurul Fitri, Raissa Friyani, Lilah Gabena Indrayani Dalimunthe Gultom, Syarifah Elena Hafizha, Putri Hasanah, Qori Hasibuan , Nabila Hasim Hasibuan, Supia Indah Hastri Kholifah Hutagalung , Septi Ratna Cempaka Hutagalung, Riris Br Hutagaol, Delvi Amira Huzeila Nisa Siregar Ihsan Fadhilah Ika Yeni Siahaan Ilmi Widya Sari Imelda Natasia Br S Meliala Indah Nasution Indah Triutami Harahap Indrayani, Gabena Junia Fitri Hayani Khofifah , Nur Kiki Rawitri Krisna Juniharta Napitupulu Leni Safriani Lestari, Sri Indah Linda, Rosa Lubis, Minda Sari Lubis, Nadya Frestika Luthfianida, Arifna M Pandapotan Nasution M Pandapotan Nasution M Pandapotan Nasution M. Alfaruqi M. Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution M. Pandapotan Nasution Mambang , D.Elysa Putri Mambang, D. Elsya Putri Mambang, Elysa Putri Maya Syafira Meutia, Cut Miftahul Jannah Minda Sar Lubis Mohd. Bilal Muhammad Amin Nasution Muhammad Amin Nasution Muhammad Ari Mukhtizar Muhammad Hizbullah Muhammad Pandapotan Nasution Muhammad Wahyu Ikhsan Muhammad Wahyu Ikhsan Munaziatul Jannah Munthe, Herlina Nasution , Muhammad Amin Nasution, Kartika Zsaskia Nasution, M. Pandapotan Nasution, Nur Sahadah Natasya, Siti Adinda Nazirah, Nazirah Nazuhra, Oca Nelsa Fahira Ningtias, Anggitha Niswa, Khairun Nofa Lismandaria Novika Utami Nur Hanifah Nuraida Nuraida Nuratika, Eli Nurliansyah, Nurliansyah Nurul Hasanah Ovalina Sylvia Br. Ginting Pertiwi, Rahma Prasetyo , Healthy Aldriany Purba , Intan Sabila Putri Puteri, Cut Intan Annisa Putri , Adilla Putri Intan Sari Putri Khairani Putri Mambang, D. Elysa Putri Rahayu, Yayuk Putri, Nadya Iwani Rafika, Intan Maya Rafita Yuniari Rafita Yuniart Rafita Yuniarti Rafliza , Rista Rahayu , Yayuk Putri Rahmadani , Adevika Rapita Yuniarti Rati , Yuliandini Rati Satri Situmorang Rawitri, Kiki Ridho, Ahmad Rasyid Ridwanto Ridwanto Ridwanto Ridwanto Ridwanto Rina Azhari Ritonga , Septi Ani Riza Syafira RR. Ella Evrita Hestiandari Rukmana, Siti Sabila , Anisa Safira , Liyuza Safitri , Nurhaliza sahrianti, Hikmah Sari, Syilvi Rinda Sarmadansyah Sarmadansyah Sartika Daulay, Anny Sasnita, Merida Selfia Lestary Sepriani, Ananda Sinaga, Novita Yulianti Siregar , Anisah Siti Aisyah Jamil Situmorang, Lusi Grasia Situmorang, Rati Satri Sony , Suyefri Sri Harti Sri Natalia Saragih Sri Ria Ranti Sri Wahyuni Sri Wahyuni Sri Wahyuni Sulwiyatul K Sani Supiyani, Supiyani Syafira, Nona Syalsabila Putri Syara, Nurdiani Syarifah Elena Gultom Tawir Rina Rezeki Tri Damaiyanti Ummi Khairani Rambe Wahdaniati , Tri Widya Sari Widya Sari Widya, Rizka Yanti, Rahma Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yayuk Putri Rahayu Yuli Apriani Br.Lubis Yuniarti , Rafita Yuniarti, Rafita Zannah , Muhamad Zikra Maqfirah Ziza Putri Aisyia Fauzi Zulmai Rani