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Journal : Journal Microbiology Science

ETHNOPHARMACY STUDY OF PLANTS WITH MEDICINAL PROPERTIES IN NORTH MALUKU PROVINCE Adrias, Cici Rahmayanti; Herwin, Herwin; Jamaluddin, Jamaluddin; Kosman, Rachmat
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.61 KB) | DOI: 10.56711/jms.v1i1.819

Abstract

North Maluku Province, especially in Joubela Village, Morotai Island Regency, South Morotai District, is one of the remote areas located in the eastern part of Indonesia. The tribes that still thrive on the island are the Galela and Tobelo, these tribes are some of the largest tribes in North Maluku, recorded to still preserve many plants as traditional medicine. The research aimed to find out the ethnopharmaceutical plants in the village of Joubela. This present study was conducted through direct observation to people who were known to understand the use of traditional medicinal plants such as shamans, community leaders and the public who have used plants for treatment which were selected through purpose sampling method. The results obtained 15 orders, 20 families, 26 genera and 26 species of plants that have empirical potential as medication and the most widely used plant as traditional medicine was garden shrub (Graptophyllum pictum L.), with the efficacy as an ulcer medicine.
IDENTIFICATION OF ACTIVE CHEMICAL COMPONENTS OF ETHANOL EXTRACT OF PAINTED NETTLE LEAVES (COLEUS SCUTELLARIOIDES L. BENTH) AGAINST GASTROINTESTINAL INFECTION BACTERIA USING TLCBIOAUTOGRAPHY AND AGAR DIFFUSION METHOD Junaedi, Fhadillah; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.004 KB) | DOI: 10.56711/jms.v3i1.869

Abstract

Infectious diseases are one of the public health problems caused by the transmission and multiplication of microorganisms. The research on the antibacterial activity of painted nettle leaves (Coleus scutellarioides L. Benth) extracts was carriedout to examine the activity and chemical components that provide inhibition against gastrointestinal infection bacteria. The extraction of painted nettles leaves performed maceration methd using a solvent of 96%. The extraction results were examined to determine the antibacterial activity at 1% concentration of the test bacteria: Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The results showed that 96% ethanol extract of painted nettle leaves inhibited Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The research conducted using agar diffusion indicated that painted nettleleaf extract showed activity against bacteria and had highly strong potential with diameters of 6-20 mm. The results of Thin Layer Chromatography (TLC) Bioautography test of ethanol extract of painted nettle leaves using chloroform:methanol eluent (3:1) showed inhibition of Escherichia coli, Shigella dysenteriae, and Salmonella thypi at Rf values of 0.90, 0.80, 0.56. Therefore, the identification test confirmed that the chemical componentsproviding antibacterial are from flavonoids, phenols, terpenoids, alkaloids, and steroidsKey words: Identification of chemical components, painted nettle leaves, gastrointestinal bacteria, TLC-Bioautography and Agar diffusion
OPTIMIZATION OF TEMPERATURE CELLULOSE PRODUCING BACTERIAL ISOLATES FROM DRAGON FRUIT (Hylocereus polyrhizus) Nurung, Ayyub Harly; Widowati, Fairuz Dyah; Herwin, Herwin; Fitriana, Fitriana
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.796 KB) | DOI: 10.56711/jms.v1i1.820

Abstract

Isolation of bacterial cellulose from fruits in traditional market of Makassar have been done. This objective of this research was to determine optimum temperature of isolate bacteria that produce cellulose from Dragon fruit (Hylocereus polyrhizus) using dilution agar method. Isolation of bacteria produced cellulose using Hestrin-Schramm agar (HSA) obtain 5 (five) isolate NB02, NB03, NS01 and NS02. The result of screening activity showed that isolate NB04 has a potential as bacteria produce cellulose. The temperature optimize of NB04 has been done at 25oC and 40oC. Based on research results showed that optimum temperature for NB04 was at 25oC and has highest cellulose content at 1,29 g.
IDENTIFICATION OF ENDOPYTIC FUNGI COMPOUND ON WHITE WEED LEAVES (Ageratum Conyzoides L.) CONTAINING THE POTENTIAL TO PRODUCE ANTIBIOTICS BY TLC-BIOAUTOGRAPHY. Rofika, Evi; Fitriana, Fitriana; Herwin, Herwin
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.835 KB) | DOI: 10.56711/jms.v3i1.894

Abstract

White weed (Ageratum conyzoides L.) is known to have antibacterial elements containing chemical compound such as saponin and flavonoids. This study aimed to examine the antibiotic activity of endophytic fungi isolates of white weed leaves by the TLC-Bioautography method. The results of endophytic fungi isolation of white weed leaves obtained 10 isolates consisting of IFDP 1, IFDP 2, IFDP 3, IFDP 4, IFDP 5, IFDP 6, IFDP 7, IFDP 8, IFDP 9, and IFDP 10. The results of the macroscopic examination of the ten isolates of endophytic fungi found different characteristics. The results of the screening using 9 samples obtained isolates that found activity showing high inhibitory power, such as IFDP 1, IFDP 2, and IFDP 4 isolates. Isolates of IFDP 1, IFDP 2 and IFDP 4 were fermented on MYB medium for 14 days. Then filtering and evaporation were carried out to produce an extract. Isolate fermentate extracts were identified using Thin Layer Chromatography with chloroform eluent: methanol (4:2). The antibiotic activity test was done using TLC-Bioautography method, and obtained an Rf value in isolate 1, at Rf 0.74 on Vibrio cholerae. Isolate 2 resulted rf value of 0.67 on Salmonella thypi, Vibrio cholerae, Eschericia coli, disentriae, Staphylococcus epidermis. Isolate 4 generated an Rf value of 0.74 on Pseudomonas aeruginosa and Vibrio cholerae. While the group of active chemical components contained in the endophytic fungi isolates of white weed leaves was lavonoids by using AlCl3 reagents, AICI3 reagent alkaloids, and Sulfuric acid for saponin identification
ISOLATION AND ACTIVITY ANTIBACTERIAL OF ISOLATES ENDOPHYTE FUNGI OF JATROPH MULTIFIDA L. STEM Herwin, Herwin; Kosman, Rachmat; Wahyuni, Sri
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.923 KB) | DOI: 10.56711/jms.v1i1.815

Abstract

Endophytic fungi that live on plant tissue has the potential to produce secondary metabolites, same as its host. This study aims to isolation endophyte fungi and antibacterial activity of isolate endophyte fungi of Jatropha multifida L. stem to Escheriacia coli and Staphylococcus aureus bacteria. The preliminary research was performed by isolation endophyte fungi of Jatropha multifida L. stem use PDAC medium and the result of isolation endophyte fungi Jatropha multifida L. stem obtained 6 isolates. Isolates endophyte fungi done examination morphology by microskopic method obtained that islolates have different carasteristic. Based on examination of antibacterial activity by agar diffusion method showed that isolates endophyte fungi that potential as antibacterial is isolate IFJT1 code where isolate IFJT1 code active to Escheriacia coli bacteria obtained inhibitory zone diameter of 17,7 mm and to Staphylococcus aureus bacteria.obtained inhibitory zone diameter of 16,7 mm
LITERATURE STUDY OF ANTIBACTERIAL ASSAY OF AVERRHOA BILIMBI L. AGAINST GRAM POSITIVE BACTERIA Sari, Erwika Dwika; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 2, No 1 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.392 KB) | DOI: 10.56711/jms.v2i1.822

Abstract

Averrhoa bilimbi L. is one of the traditional medicines that people empirically use as medicine to treat various diseases such as cough, diabetes, rheumatism, mumps, canker sores, toothache, bleeding gums, acne, diarrhea to high blood pressure. The research aimed to determine the antibacterial activity of Averrhoa bilimbi L. against gram-positive bacteria. This research applied a literature study method in which the references were obtained from 3 data bases, namely google scholar, pubmed and mdpi, then selected journals that metthe criteria in the literature study.  From the journal exploration, 7 journals were obtained. Then the antibacterial activity of Averrhoa bilimbi L. against gram-positive bacteria was observed. The results confirmed that the fruit and leaves of Averrhoa bilimbi L. had an antibacterial activity against Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, and Propionibacterium acnes due to the presence of secondary metabolites, namely saponins, tannins, alkaloids and flavonoid.
LITERATURE STUDY OF ANTIBACTERIAL ACTIVITY OF THE FAMILI RUTACEA Nurfadila, Nurfadila; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 2, No 1 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.376 KB) | DOI: 10.56711/jms.v2i1.823

Abstract

Indonesia has one of potential plants as an herbal medicine, called Lime (Citrus aurantifolia) and Kaffir Lime (Citrus hystrix DC), both used for different purposes such as cooking, seasoning and traditional medicine. Both plants are derived from the family of Rutaceae which belongs to shrubs that have many branches and twigs. This study aimed to carry out the antibacterial assay of the Rutaceae family. This study used a literature study approach, which references were obtained through 3 Journal sources, namely Google scholar, pubmed, and thscience direct. Furthermore the selected research article which met the criteria was reviewed. Based on the search done, it obtained 7 articles and identified antibacterial activity of lime and Kaffir Lime. The result confirmed that both have antibacterial compounds against bacteria Escherichia coli, and Staphylococcus aureus because they contain secondary metabolite compounds consisting of alkaloids, flavonoids, tannins, and essential oils.
ISOLATION OF SKY ROOT ENDOPHITE (Helminthostachys zeylanica (L.) Hook) PRODUCING ANTIBIOTICS DIFFERENTLY AGAR Mitra, Andi; Herwin, Herwin; Nuryanti, Siska
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.907 KB) | DOI: 10.56711/jms.v3i1.952

Abstract

IDENTIFIKASI KOMPONEN KIMIA EKSTRAK ETANOL DAUN PACAR KUKU (Lawsonia inermis L.) SEBAGAI ANTIBAKTERI DAN ANTIOKSIDAN Herwin, Herwin; Nurung, Ayyub Harly; Ambon, Nur Intan; Naid, Tadjuddin
Journal Microbiology Science Vol 2, No 1 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.637 KB) | DOI: 10.56711/jms.v2i1.824

Abstract

Prevention of the transmission of covid 19 can be done by implementing the health protocol, namely 3M (wearing masks, washing hands and social distance). Based on research, the use of masks can reduce the risk of transmission of COVID-19 by 45 percent as well as hand washing reduces the risk by 35 percent. WHO recommends washing hands with soap/antiseptic for 20-30 seconds and applying the right steps. Another effort is to maintain a distance of at least 1 meter and avoid crowds. To assist the government in preventing the transmission of covid 19, socialization of the 3M activity was carried out at the Darul Istiqamah Maros Islamic Boarding School which is located on the Maros-Makassar Street, Mandai District, Maros Regency. This location was chosen because Maros was the third highest area with the number of people who are positive for the corona virus in South Sulawesi. Islamic boarding school students are a partner group that is expected to apply the 3M health protocol so as to prevent the transmission of covid 19. The activity is carried out by providing counseling about the importance of implementing 3M, how to implement 3M and also by distributing masks, soap and disinfectants needed to implement health protocols. The activity was carried out well and received a good response from the participants with 33 participants from various classes who were students of the Darul Istiqamah Islamic Boarding School Makassar.
Antibacterial Activity of Compound TL-4 IFATL-05 Isolate Endophytic Fungi from Tunjuk Langit Root (Helminthostachys zeylanica (L.) Hook) Against Bacterial Gastrointestinal Tract Infection Yuridu, Fatur Rahmansyah; Herwin, Herwin; Fitriana, Fitriana
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.983

Abstract

Tunjuk Langit Plant (Helminthostachys zeylanica (L.) Hook) contains a lot of secondary metabolite compounds, one of which is found in the root which contain the flavonoids ugonin and quercetin. The purpose of this study was to determine the antibacterial activity of compound 4 isolates IFATL-05 endophytic fungi from Tunjuk Langit (Helminthostachys zeylanica (L.) Hook) root against bacterial gastrointestinal tract infection. The results of purification of endophytic fungi with IFATL-05 code on macroscopic examination showed blackish-white fungus color, round with scalloped colony shapes, wavy edges and drop-like elevation angles. The process of separating the compounds using KLTP with n-hexane:ethyl acetate (1:1) as an eluent resulted in four bands of active compounds in UV lamps of 254 and 366 nm (TL-1, TL-2, TL-3 and TL-4). Test results for compound 4 isolates of IFATL05 endophytic fungi from Tunjuk Langit root (Helminthostachys zeylanica (L.) Hook) using the Bioautographic TLC method using n-hexane:ethyl acetate (6:1) as eluent on Escherichia coli, Salmonella thypi, Shigella dysenteriae and Vibrio cholerae with Rf value = 0.90
Co-Authors -, Dwi Haryono Abadi, Ferryal Abdul Alim Achmad Rosyadi Yusuf Hibai, Achmad Rosyadi Yusuf Adrias, Cici Rahmayanti Afdal Afdal, Afdal Agustin Agustin Agustin Ahsan, Miftahul Khair Alfahral, Reza Ambon, Nur Intan Andesa, Khusaeri Andika, Ilham Angeline angeline Anggraini, Subekti Anggraini, Subekti Wining Anwar, Fozi Arifa, Noorul Asan, Adrianus Kia Askara, Ghanisa Anggit Awaluddin, M Awan Hariono Bahtiar Bahtiar Bantulu, Ananta Rahayu Bely, Bely Budi Aryanto Budiharti Budiharti Candra Tri Utami Chandra Kusuma Wibawa Chintya, Ora Christina Fajar Sriwahyuniati Dahalan, Shakila Che Dwi Haryono Edwar Ali Enny Zubaidah Erlin Ernawati Ernawati Eva Johannes Fadli Ihsan Fahirah, Jihan Fahzri, Muhamad Faidillah Kurniawan Farida Agus Setiawati Feri Dwi Astuti, Feri Dwi Firna Anggriani Mile, Firna Anggriani Fitriana Fitriana Fransiskus Zoromi Fransiskus Zoromi, Fransiskus Gultom , Polter Gultom, Iwan J.P Gusmanida, Gugun Haidir, Muhammad Hamdani - Handika, Marezi hanudin, sub Haryani Haryani Hasan Hasan Helda Yenni, Helda hikma, Nurhikma Hikmah, Annafi'atul Ilham Wahyudi Indriana Rahmawati Jamaluddin Jamaluddin Jhon, Winarni Julianti, Selly Junadhi Junadhi Junadhi, Junadhi Junaedi, Fhadillah Karhani, Muna Karmini Karmini Karyati Karyati Kasyfillah, Muhammad Haidarsyah Khusaeri Andesa Khusaeri Andesa Koko Harianto Kurniawanto, Eko Kurniawati Kurniawati Kusumawati, Tri Harum Letisia, Maria Lydia Surijani Tatura M Destiani, Deassy M. Wino Adi Putra Mardainis Mardianis , Mardianis Mariah Mariah, Mariah Meilani Meilani, Meilani Mitra, Andi Monika, Arum Muhammad Said Husin Muhammad, Farhan Muldin, Nurul Insani Muzakkir Baits, Muzakkir Nabillah, Rokhmatun Naim, Nurul Khatimah Nawan Primasoni nirwana rasyid, nirwana Noviana Noviana Novitasari, Welly Nugroho, Alnico Imam Nur Faizin - Nur, Wardah Rafidah Nurfadila Nurfadila, Nurfadila Nurjayadi Nurjayadi Nurung, Ayyub Harly Nurwirhanuddin, Nurwirhanuddin Pasaribu, Rechman Pramesti , Selviana Dewi Purwaningsih, Arifah Yuli Putra, Jean Riko Kurniawan Putra, Rahman Pranovri PUTRI WULANDARI R. Rusli Rachmat Kosman, Rachmat Rafael Billy Leksono, Rafael Billy Rahayu Condro Murti Rahmanto, Ihwan Rahmayanti, Hani Rahmayanti Ramadhana, Evi Rahma Rasul, A. Rasul Ratna Budiarti Rendi Rendi, Rendi Renyaan, Anastasya Ricki, Ricki Ririn Ririn Rita Eka Izzaty, Rita Eka Rizki Ramadhani Rizky, Deo Alfa Roehan, Muhammad Sani Rofika, Evi Rometdo Muzawi, Rometdo Rudy Tandra Samudera, Arung Saputra, Richard Sari, Erwika Dwika Sari, Felisa Ananda Selpida Handayani, Selpida Selviati, Ratmi Septianingtias, Arina Siga, Adolfus Siktus Siska Nuryanti, Siska Siti Rohmah Nurhayati Sjafaraenan Sjafaraenan, Sjafaraenan Sompa, Putri Tenri Sri Wahyuni Subagyo Irianto Sugiono Sugiono Suhendar Suhendar, Suhendar Sujati, Hironimus Supartinah Supartinah Susandri, Susandri Susanti, Susanti Syamsiar, Syamsiar Tadjuddin Naid Tandukar, Kritika Torkis Nasution Tri Hadi Karyono Trisakti, Jhon Citro Triyani Arita Fitri Tuwu, Darmin Umafagur, Dea Andela Vina Meliana, Vina Vincent Vincent, Vincent Waruwu, Jefnita Sain Widiyanto Widiyanto Widowati, Fairuz Dyah Wirawan, Alif Yusra Wuri Wuryandani Yahya, Fifi Adelia Yoel Yoel Yoga Yuniadi Yuridu, Fatur Rahmansyah