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Journal : Journal Microbiology Science

Antibacterial Activity of Extra Ethanol Kopasanda Leaves (Chromolaena Odorate L.) Against Pathogenic Bacteria of Urinary Tract Infection by TLC-Bioautography and Agar Diffusion Wirawan, Alif Yusra; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.979

Abstract

Kopasanda  (Chromolaena odorata L.) leaves are one of the plants (herbs) containing various types of secondary metabolites, including saponins, tannins, alkaloids, and flavonoids, which function as antibacterial agents used as wound medicine for the community. This study aims to determine the antibacterial activity of the ethanol extract of Kopasanda (Chromolaena odorate L.) leaves using the TLC-bioautography and diffusion methods. The results of the antibacterial activity screening test obtained the active extract at a concentration of 0.1% against Escherichia coli and Staphylococcus aureus. The results of the antibacterial activity test using the TLC-Bioautography method with the eluent N-Hexane:Ethyl Acetate (4:1) showed results from Staphylococcus aureus bacteria with Rf values = 0.90, 0.78, 0.67, 0.52, 0.38, 0.27, 0.14, 0.05, and Escherichia coli bacteria with Rf values = 0.90, 0.52, 0.38, 0.27, 0.14, 0.05. Based on the results of testing the antibacterial activity with the agar diffusion method, the largest diameter of the inhibition zone at a concentration of 10% was obtained for Escherichia coli bacteria with an inhibition zone diameter of 13.59 mm, and Staphylococcus aureus with an inhibition zone diameter of 15.52 mm.
Antibacterial Activity of Ethanol Extract of Purple Kencana Leaves (Ruellia tuberosa L.) Using TLC-Bioautography and Agar Diffusion Methods Sari, Felisa Ananda; Herwin, Herwin; Handayani, Selpida
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.982

Abstract

Kencana Ungu Leaf (Ruellia tuberosa L.) is a traditional plant that has antibacterial activity. The purpose of this study was to determine the antibacterial activity of the ethanol extract of Kencana Ungu leaves (Ruellia tuberosa L.) using TLC-Bioautography and Agar Diffusion methods. Simplisia from Kencana Ungu leaves (Ruellia tuberosa L.) was extracted by maceration method and then evaporated to obtain a thick extract. Preliminary testing, namely the antibacterial screening test, active extract at a concentration of 0.5% against Escherichia coli, Shigella dysenteriae, and Salmonella thypi. The results of the antibacterial activity test using the TLC-Bioautography method using chloroform: methanol (7:1) as an eluent showed results with an Rf value of 0.89 which inhibited the growth of Escherichia coli, Shigella dysenteriae, and Salmonella thypi bacteria. While the results of testing the antibacterial activity using the Agar Diffusion method obtained the largest diameter of the inhibition zone at a concentration of 20% for Escherichia coli bacteria with an inhibition zone diameter of 12.15 mm, Shigella dysenteriae with an inhibition zone diameter of 11.72 mm, and Salmonella thypi with an inhibition zone diameter of 12.67 mm
Isolation Of Endophytic Fungi From Aloe Vera Against Bacteria That Cause Digestive Tract Infections By TLC-Bioautography And Agar Diffusion Ramadhana, Evi Rahma; Herwin, Herwin; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.993

Abstract

The Aloe vera plant, also known as the Lidah buaya plant, has antibacterial properties due to its secondary metabolites, which include alkaloids, flavonoids, tannins, and saponins. In this study, endophytic fungi from Lidah Buaya (Aloe vera) were isolated against bacteria that cause gastrointestinal tract infections using TLC-Bioautography and Agar Diffusion. In this study, 7 isolates of endophytic fungi were obtained, one of which was IFLB 1 which had potential as an antibacterial, purification of the endophytic fungi was carried out and then macroscopic examination was carried out on the fungi to see the colonies formed. After that, a screening test was carried out for isolates of endophytic fungi. Then the isolates that gave the best activity were reproduced and continued with the fermentation process for 14 days. The extraction results were then evaporated to obtain a thick extract and a Thin Layer Chromatography (TLC) Identification test was carried out in which the eluent used was chloroform: methanol (5:1). Then continued the TLC-Bioautographic Antibacterial Activity Test on Escherichia coli, Salmonella thypi, Shigella dysentriae and Vibrio cholerae bacteria and obtained 2 active spots with Rf1 = 0.78 and Rf2 = 0.30. While the results of testing the antibacterial activity using the Agar Diffusion method obtained the largest diameter of the inhibition zone at a concentration of 4% for Escherichia coli with an inhibition zone diameter of 15.80 mm, Salmonella thypi with an inhibition zone diameter of 14.90 mm, Shigella dysentriae with an inhibition zone diameter of 13 .85 mm and Vibrio cholerae with an inhibition zone diameter of 14.70 mm
Antibacterial Activity of Tunicata Ethanol Extract Polycarpa Aurata gainst Escherichia Coli and Salmonella Typhi by TLC-Bioautography and Agar Diffusion Selviati, Ratmi; Herwin, Herwin; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1040

Abstract

Tunicata Polycarpa aurata contains chemical compounds in the form of peptides and alkaloids  that are cytotoxic and have the ability as antibacterial against several pathogenic bacteria. The aims of this study was to determine the antibacterial activity of the ethanol extract of Tunicata Polycarpa aurata using the TLC-bioautography and diffusion method. Tunicata Polycarpa aurata was extracted by maceration and then evaporated to obtain a thick extract. The results of the antibacterial screening test showed the active extract at a concentration of 0.1% against Escherichia coli and Salmonella typhi. The results of testing for antibacterial activity using the TLC-bioautography with using ethanol: ethyl acetate (1:4) eluent showed results of Salmonella typhi bacteria with an Rf value = 0.27 and Escherichia coli bacteria with an Rf value = 0.27. While the results of testing the antibacterial activity the using the agar diffusion method showed the largest inhibition zona diameter at a concentration of 4% for Escherichia coli bacteria with an inhibition zone diameter of 12.55 mm, and Salmonella typhi with an inhibition zone diameter of 12.3 mm. Phytochemical screening tests on the ethanol extract of Tunicata Polycarpa aurata revealed  the presence of flavonoids, tannins, steroids/terpenoids, alkaloids and saponins.
Antibacterial Activity of Ethanol Extract of Sirih Cina Leaves (Peperomia pellucida) Using TLC-Bioautography and Agar Diffusion Methods Ahsan, Miftahul Khair; Herwin, Herwin; Rusli, Rusli
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1058

Abstract

Sirih cina leaf (Peperomia pellucida) is a plant that contains active compounds of alkaloids, terpenoids, tannins, saponins, flavonoids and phenols that have potential antibacterial activity. This research aimed to determine the antibacterial activity of ethanol extract of sirih cina leaves (Peperomia pellucida) using the TLC-Bioautography and Agar Diffusion methods. Sirih cina leaf (Peperomia pellucida) was extracted by maceration and evaporated to obtain a thick extract. Screening test result showed that the extract was active at a concentration of 0.1% against the bacteria Propionibacterium acnes, Staphylococcus aureus and Pseudomonas aeruginosa. The results of antibacterial activity testing using the TLC-Bioautography method using the eluent methanol: chloroform (1:4) showed results with Rf values = 0.94, 0.80, 0.65, 0.54, 0.40, 0.30, 0.21, 0.10 against the bacteria Propionibacterium acnes, Staphylococcus aureus, and Pseudomonas aeruginosa. In determining the MIC, the value obtained was the same for the three test bacteria at a concentration of 0.5%, while the KBM value was at a concentration of 2% for Propionibacterium acnes bacteria, a concentration of 0.5% for Staphylococcus aureus bacteria, and a concentration of 1% for Pseudomonas aeruginosa bacteria. The results of antibacterial activity testing using the Agar Diffusion method obtained the largest inhibitory zone diameter at a concentration of 16% against Propionibacterium acnes bacteria with an inhibitory zone diameter of 23.20 mm, Staphylococcus aureus bacteria with an inhibitory zone diameter of 23.63 mm, and Pseudomonas aeruginosa bacteria with an inhibitory zone diameter. 24.48mm
Antibacterial Activity of N-Hexan and Ethyl Acetate Fractions of Gaharu (Aquilaria Malaccensis) Leaves against Bacteria that Cause Infection Yahya, Fifi Adelia; Herwin, Herwin; Fitriana, Fitriana
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1139

Abstract

Gaharu (Aquilaria malaccensis) leaves contain alkaloids, terpenoids, flavonoids, saponins, and tannins that can help cure infections. This study aims to determine the antibacterial activity of n-hexane and ethyl acetate fractions of Gaharu leaves against skin infection bacteria such as Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis by Bioautography-TLC. Gaharu leaves symplisia was extracted using the maceration method using ethanol 96% solvent and fractionation with solid-liquid method to obtain n-hexane and ethyl acetate fractions. The result of the screening antibacterial activity of n-hexane and ethyl acetate fractions of Gaharu leaves with concentrations of 0,5% active to 4 bacteria test. The results of antibacterial activity n-hexane and acetaet ethyl fraction by Bioautography-TLC using n-hexane:ethyl acetate (1:4) eluent showed Rf 0.67 and Rf 0,16 value in the n-hexane fraction and an Rf 0.78 value in the ethyl acetate fraction active to 4 bacteria test.  The identification results of the chemical compound of the n-hexane fraction contain flavonoids and tannins (AlCl3 and FeCl3 reagents.), the ethyl acetate fraction contains flavonoids ( AlCl3 reagents).
Antibacterial Activity of N-Hexan and Ethyl Acetate Fractions of Gaharu Leaves (Aquilaria malaccensis) Against Bacteria Causing Skin Infection Sompa, Putri Tenri; Herwin, Herwin; Fitriana, Fitriana
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1147

Abstract

Gaharu (Aquilaria malaccensis) leaves are plants that are useful in the treatment of infectious diseases. This study aims to obtain n-hexane and ethyl acetate fractions of Gaharu leaves that have antibacterial activity against skin infection bacteria by agar diffusion. The results of antibacterial activity screening of the n-hexane fraction and ethyl acetate fraction at a concentration of 0.1% is bacteriostatic. The test results of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) using concentrations of 0.1%, 0.2%, 0.4%, 0.8%, 1.6%, 3.2%, 6.4% and 12.8% obtained MIC value of n-hexane fraction at 0.2% against P. acnes, P. aeurogynes bacteria and 0.4% against S. aureus, S. epidermidis bacteria, MIC value of ethyl acetate fraction was 0.2% against P. aeuroginosa, S. aureus bacteria and 0.4% against P. acnes, S. epidermidis bacteria. MBC value of n-hexan fraction was 0.4% against P. acnes, P. aeuroginosa bacteria, 0.8% against S. aureus bacteria, 1.6% against S. epidermidis bacteria, MBC value of ethyl acetate fraction was 0.4% against P. acnes, P. aeuroginosa, S. aureus bacteria, 0.8% against S. epidermidis bacteria. The results of the antibacterial activity using concentrations of 1.56%, 3.12%, 6.25%, 12.5% and 25% obtained the largest inhibition zone diameter of the n-hexane fraction was 13,99 mm and the ethyl acetate fraction was 27,68 mm against S. epidermidis bacteria at a concentration of 25%. Based on the results, it can be concluded that the antibacterial activity of the ethyl acetate fraction against all test bacteria is stronger than the n-hexane fraction
Antibacterial Activity Test of Ceremai Leaf Ethanol Extract (Phyllanthus acidus (L.) Skeels) Against Bacteria Causing Skin Infections by KLT-Bioautography and Agar Diffusion Naid, Tadjuddin; Herwin, Herwin; Muldin, Nurul Insani
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1204

Abstract

Ceremai leaves (Phyllanthus acidus) are known for their medicinal properties, including the treatment of infectious diseases. This study aims to evaluate the antibacterial activity of ethanol extract from Ceremai leaves against skin infection-causing bacteria using Thin Layer Chromatography-Bioautography (TLC-Bioautography) and agar diffusion methods. The study began with an antibacterial screening test, followed by TLC-Bioautography, and then determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) at various concentrations (0.125% to 16%). The agar diffusion method was used to further assess antibacterial activity. Screening results showed antibacterial activity at a concentration of 1%. TLC-Bioautography with a chloroform: methanol: water (20:2:0.5) eluent revealed active spots with Rf values of 0.8, 0.54, 0.36, and 0.29. The MIC values were 0.25% for P. acnes and S. aureus, and 0.5% for P. aeruginosa and S. epidermidis. The MBC values were 0.5% for P. acnes and S. aureus, and 1% for P. aeruginosa and S. epidermidis. The largest inhibition zone in the agar diffusion test was observed at a 32% concentration against S. aureus, measuring 20.11 mm. These findings suggest that Ceremai leaf extract has significant antibacterial potential and could be considered for developing alternative treatments for skin infections.
Antibacterial Activity of Etanol Extract of Pinang (Areca catechu L.) Clup against Streptococcus mutans and Porphyromonas gingivalis Nuryanti, Siska; Herwin, Herwin; Rahmayanti, Hani Rahmayanti
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1178

Abstract

Areca nut (Areca catechu L.) is a plant that has many uses and can be used for traditional medicine. Areca nut skin can be used to treat indigestion (dyspepsia), edema and beriberi due to low urine output. This study aims to determine the antibacterial activity of ethanol extract of areca nut peel against the test bacteria Streptococcus mutans and Porphyromonas gingivalis. In this research, the areca nut peel simplicia was extracted using the maceration method with 96% ethanol solvent, after which a liquid ethanol extract was obtained and then evaporated using a rotary vacuum evaporator to obtain a thick extract. After the extract is thick, a Minimum Inhibitor Concentration (MIC) test and a Minimum Bactericide Concentration (MBC) test are carried out. The results obtained in the Minimum Inhibitor Concentration (MIC) test and Minimum Bactericide Concentration (MBC) test on Streptococcus mutans and Porphyromonas gingivalis bacteria were 0.5%. Antibacterial activity test using the agar diffusion method was obtained, for Streptococcus mutans bacteria, the largest inhibitory zone diameter at a concentration of 16% was 12.58 mm and for Porphyromonas gingivalis bacteria at a concentration of 16%, namely 10.93 mm. Based on research, ethanol extract of areca nut peel has antibacterial potential.
Characterization of Cellulose-Producing Bacterial Isolates from the Areca Nut (Areca catechu L.) Nuryanti, Siska; Herwin, Herwin; hikma, Nurhikma
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1179

Abstract

Areca nut peel (Arecha catechu L.), a byproduct of areca nut processing, holds potential as a raw material for bioplastics through cellulose utilization. This study aimed to identify bacterial species in areca nut peel capable of producing cellulose. Employing bacterial isolation and identification methods, including morphological and biochemical tests, four bacterial isolates were obtained. Two isolates, identified as gram-negative, were classified under the genus Acetobacter, and two gram-positive isolates were identified as belonging to the genus Lactobacillus. These findings demonstrate the potential use of cellulose from areca nut peel in bioplastic production.
Co-Authors -, Dwi Haryono Abadi, Ferryal Abdul Alim Achmad Rosyadi Yusuf Hibai, Achmad Rosyadi Yusuf Adrias, Cici Rahmayanti Afdal Afdal, Afdal Agustin Agustin Agustin Ahsan, Miftahul Khair Akhmad Akhmad Alfahral, Reza Alrif, Syaharuddin Amaliah, Ema Ambon, Nur Intan Andesa, Khusaeri Andi Mulyadi Andika, Ilham Angeline angeline Anggraini, Subekti Anggraini, Subekti Wining Anshary, Muhammad Ridha Anwar, Fozi Arifa, Noorul Asan, Adrianus Kia Askara, Ghanisa Anggit Awaluddin, M Awan Hariono Bahtiar Bahtiar Bantulu, Ananta Rahayu Bely, Bely Budi Aryanto Budiharti Budiharti Candra Tri Utami Chandra Kusuma Wibawa Chintya, Ora Christina Fajar Sriwahyuniati Dahalan, Shakila Che Dwi Haryono Edwar Ali Enny Zubaidah Erlin Ernawati Ernawati Eva Johannes Fadli Ihsan Fahirah, Jihan Fahrika, A. Ika Fahzri, Muhamad Faidillah Kurniawan Farida Agus Setiawati Feri Dwi Astuti, Feri Dwi Firna Anggriani Mile, Firna Anggriani Fitriana Fitriana Fransiskus Zoromi Fransiskus Zoromi, Fransiskus Gultom , Polter Gultom, Iwan J.P Gusmanida, Gugun Haidir, Muhammad Hamdani - Handika, Marezi hanudin, sub Haryani Haryani Hasan Hasan Helda Yenni, Helda hikma, Nurhikma Hikmah, Annafi'atul Hikmah, Arina Nur Ilham Wahyudi Indriana Rahmawati Jamaluddin Jamaluddin Jhon, Winarni Julianti, Selly Junadhi Junadhi Junadhi, Junadhi Junaedi, Fhadillah Karhani, Muna Karmini Karmini Karyati Karyati Kasyfillah, Muhammad Haidarsyah Khusaeri Andesa Khusaeri Andesa Koko Harianto Kurniawanto, Eko Kurniawati Kurniawati Kusumawati, Tri Harum Letisia, Maria Lukman, Adli Lydia Surijani Tatura M Destiani, Deassy M. Wino Adi Putra M. Yogi Riyantama Isjoni Mardainis Mardianis , Mardianis Mariah Mariah, Mariah Meilani Meilani, Meilani Mitra, Andi Monika, Arum Muhammad Said Husin Muhammad, Farhan Muldin, Nurul Insani Muzakkir Baits, Muzakkir Nabillah, Rokhmatun Naim, Nurul Khatimah Nawan Primasoni nirwana rasyid, nirwana Noviana Noviana Novitasari, Welly Nugroho, Alnico Imam Nuha, Uli Nur Faizin - Nur, Wardah Rafidah Nurfadila Nurfadila, Nurfadila Nurjayadi Nurjayadi Nurung, Ayyub Harly Nurwirhanuddin, Nurwirhanuddin Pasaribu, Rechman Pramesti , Selviana Dewi Purwaningsih, Arifah Yuli Putra, Jean Riko Kurniawan Putra, Rahman Pranovri PUTRI WULANDARI Qureshi, Muhammad Saifullah R. Rusli Rachmat Kosman, Rachmat Rafael Billy Leksono, Rafael Billy Rahayu Condro Murti Rahmanto, Ihwan Rahmayanti, Hani Rahmayanti Ramadhana, Evi Rahma Rasul, A. Rasul Ratna Budiarti Ratna Purwanti, Indah Razak, Syaparuddin Rendi Rendi, Rendi Renyaan, Anastasya Ricki, Ricki Ririn Ririn Rita Eka Izzaty, Rita Eka Rizki Ramadhani Rizky, Deo Alfa Roehan, Muhammad Sani Rofika, Evi Romaida Efrianti Br Situmorang, Yustina Rometdo Muzawi, Rometdo Rosita Endang Kusmaryani Rudy Tandra Rusman, Haerusman Samudera, Arung Saputra, Richard Sari, Erwika Dwika Sari, Felisa Ananda Selpida Handayani, Selpida Selviati, Ratmi Septianingtias, Arina Siga, Adolfus Siktus Siska Nuryanti, Siska Siti Rohmah Nurhayati Sjafaraenan Sjafaraenan, Sjafaraenan Sompa, Putri Tenri Sri Wahyuni Subagyo Irianto Sugiono Sugiono Suhendar Suhendar, Suhendar Sujati, Hironimus Supartinah Supartinah Susandri, Susandri Susanti, Susanti Syamsiar, Syamsiar Tadjuddin Naid Tandukar, Kritika Torkis Nasution Tri Hadi Karyono Trisakti, Jhon Citro Triyani Arita Fitri Tuwu, Darmin Umafagur, Dea Andela Vina Meliana, Vina Vincent Vincent, Vincent Waruwu, Jefnita Sain Wenehenubu, Fitalis Ekhy Widiyanto Widiyanto Widowati, Fairuz Dyah Widyatmike Gede Mulawarman Wirawan, Alif Yusra Wuri Wuryandani Yahya, Fifi Adelia Yoel Yoel Yoga Yuniadi Yuridu, Fatur Rahmansyah