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All Journal ACTA VETERINARIA INDONESIANA Media Veteriner Jurnal Kedokteran Brawijaya Jurnal Edukasi dan Sains Biologi Jurnal Ilmiah Mahasiswa Veteriner AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh Narra J Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Jurnal Medika Veterinaria Jurnal Agripet
Muslim Akmal
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Role of forkhead box protein 2 (FOXP2) in oral-motor abilities of preterm infants: A brief literature review Mauliza , Mauliza; Dimiati, Herlina; Akmal, Muslim; Imran, Imran
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.237

Abstract

Preterm infants, born before the 37-week gestation period, have limited storage for nutrients at birth and are vulnerable to poor feeding, severe nutritional deficits and growth retardation. The immature gastrointestinal system leads preterm infants to experience a delay in initiating enteral nutrition. Inappropriate feeding can cause acute and long-term morbidity, prolonged hospitalization and increased treatment cost. Generally, preterm infants that are born after 32 weeks of gestation without severe comorbidities do not have dysphagia and should start oral feeding soon after birth. Preterm infants should have well-developed sucking-swallowing-breathing coordination by 32–34 weeks of gestational age. However, some infants take days or weeks to master the skill. The oral feeding development involves forkhead box protein 2 (FOXP2)-expressing neurons that are found in the deep layers of the cortex, basal ganglia, parts of the thalamus and Purkinje cells of the cerebellum. In mammals, these areas belong to the brain network circuits working for motor coordination in learning and acquiring sensorimotor skills. This review aimed to describe the role of FOXP2 in oral-motor skills in preterm infants, including oral feeding, sucking-swallowing-breathing coordination and language development. The oral-motor skills development could be an early predictor for language delay in premature infants, representing a vulnerable group susceptible to such delays.
Effect of black garlic (Allium sativum) on gonadosomatic index, follicle-stimulating hormone level and spermatozoa quality: A study in monosodium glutamate-exposed rat model Bani, Ika I.; Zulkarnain, Zulkarnain; Gholib, Gholib; Syahrizal, Dedy; Nugraha, Taufiq P.; Ramadhan, Akmal; Akmal, Muslim; Maulina, Nora
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.617

Abstract

Infertility rates have risen significantly, one of which is due to monosodium glutamate (MSG) consumption. Recent studies have shown that flavonoids in black garlic (Allium sativum) act as antioxidants. The aim of this study was to assess the effect of black garlic extract (BGE) on gonadosomatic index, follicle-stimulating hormone (FSH) levels, and spermatozoa quality in rats exposed to MSG. Twenty-five healthy rats, aged ten to twelve weeks, were divided equally into five experimental groups: (1) negative control (NC), no intervention; (2) positive control (PC), fed with MSG 8 mg/kg; and (3) fed with MSG + BGE 200 mg/kg; (4) fed with MSG + BGE 400 mg/kg; and (5) fed with MSG + BGE 600 mg/kg. Oral MSG was administered once a day for two weeks before BGE administration was started for two weeks. The measured endpoints were gonadosomatic index, FSH levels, and spermatozoa concentration and quality (spermatozoa motility and abnormality). Analysis of variance (ANOVA) followed by Duncan's post hoc analysis was used to assess the measurement differences. The result suggested that the administration of BGE did not significantly affect the gonadosomatic index (p=0.513). Significant decreases in FSH levels (p=0.005) and spermatozoa concentration were observed in the PC group compared to other groups (p<0.001). Additionally, spermatozoa motility was significantly lower in the PC group compared to NC, BGE200, BGE400, and BGE600 (p<0.001), with higher motility noted in BGE200, BGE400, and BGE600 compared to PC (p<0.001). Furthermore, PC had significantly higher spermatozoa abnormalities compared to NC, BGE200, BGE400, and BGE600 (p<0.001). In conclusion, administration of BGE had a significant effect on the improvement of FSH levels and the quality of spermatozoa in rats exposed to MSG.
Black garlic exhibited hepatoprotective effect against monosodium glutamate-induced hepatotoxicity in animal model Maulina, Nora; Zachreini, Indra; Gholib, Gholib; Suwandi, Abdulhadi; Akmal, Muslim
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.799

Abstract

Monosodium glutamate (MSG) is commonly used as a flavor-enhancing agent in foods, and studies have demonstrated its toxic effects in animal models. Black garlic is known for its antioxidant and anti-inflammatory properties; however, there is a lack of studies on the potential hepatoprotective effect of black garlic ethanol extract (BGE) against MSG-induced hepatotoxicity in rats. The aim of this study was to investigate the hepatoprotective effects of ethanol extract of black garlic against MSG-induced liver damage in animal model. Twenty-five male Wistar rats were randomly assigned to five groups (n=5): negative control, MSG only, and MSG with three different doses of BGE. The MSG only and MSG with BGE groups were orally administered with 8 mg/kg MSG daily. After MSG treatment, the MSG with BGE groups received BGE orally at daily doses of 200, 400, or 600 mg/kg body weight for 16 consecutive days. Subsequently, the levels of serum liver enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), interferon-gamma (IFN-γ), and cyclooxygenase-2 (COX-2) were measured. Our data indicated that the group treated with 200 mg/kg BGE had significant lower levels of AST and ALT significantly compared to the MSG-only group. The MSG-treated group had higher levels of the inflammatory markers COX-2 and IFN-γ, which were lowered by administration of 200 mg/kg BGE. In contrast, higher doses of BGE led to greater levels of COX-2 and IFN-γ compared to those in the MSG-only group. This study suggested that BGE might have hepatoprotective effects at low dose, potentially mitigating MSG-induced liver damage. However, the higher dose of black garlic extract did not alleviate inflammation, as shown by the higher levels of COX-2 and IFN-γ.
Histologi, Histomorfometri, dan Histokimia Usus Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas Bagus Dwijayanti; Erdiansyah Rahmi; Ummu Balqis; Fitriani Fitriani; Dian Masyitha; Dwinna Aliza; Muslim Akmal
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.16012

Abstract

ABSTRACT. Usus adalah salah satu organ pencernaan yang berperan penting pada proses absorpsi nutrisi sebagai sumber energi. Penelitian ini bertujuan mengetahui histologi, histomorfometri dan histokimia sebaran glikogen pada usus ayam buras (Gallus gallus domesticus) sebelum dan setelah menetas menggunakan pewarnaan Haematoksilin-Eosin (HE) dan pewarnaan Periodic Acid Schiff (PAS). Sampel penelitian terdiri atas empat kelompok tingkat umur berbeda, setiap kelompok berjumlah enam usus ayam. Hasil penelitian menunjukkan perbedaan nyata (P0,05) morfometri usus ayam buras secara makroskopis. Secara histologis pada setiap perkembangan sudah terlihat lapisan usus yang terdiri atas tunika mukosa, submukosa, muskularis, dan serosa. Pada tunika mukosa, didapati tiga lamina yaitu lamina epitelia, propria, dan muskularis mukosa yang mulai teramati pada masa inkubasi hari ke-14. Lamina epitelia berupa sel epitel silindris selapis dengan mikrovili, lamina propria berupa jaringan ikat, dan lamina muskularis mukosa berupa berkas otot polos. Pada tunika mukosa dijumpai adanya sel Goblet dan kripta Liberkuhn yang teramati pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. Hasil histomorfometri ketebalan lapisan usus, panjang vili, lebar vili, dan diameter lumen usus ayam buras pada masa inkubasi dan setelah menetas menunjukkan perbedaan nyata (P0,05). Temuan reaksi positif kuat terhadap pewarnaan PAS dijumpai pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7 dan positif lemah pada masa inkubasi hari ke-14. Dapat disimpulkan bahwa terdapat korelasi antara perkembangan usus ayam secara makroskopis, histomorfometri ketebalan tunika dan vili, serta sebaran reaksi positif kuat terhadap pewarnaan PAS pada masa inkubasi hari ke-20 dan setelah menetas hari ke-7. (Histological, histomorphometrical, and histochemical of intestine on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRAK. The intestines are one of the digestive organ that play an important role in the nutrient absorption process as an energy source. The aim of this study was to determine the histomorphometric and histochemical of glycogen distribution in the intestines of native chickens pre and post hatching periods using Haematoxylin-Eosin (HE) staining and Periodic Acid Schiff (PAS) staining. The samples of this study was consisted of four different age groups, each group consisting of six chicken intestines. The results showed a significant difference (P0.05) on macroscopic morphometry of the native chickens intestine. Histologically in each group has been found the intestines consisting of mucosa, submucous, muscularis, and serous tunic. In the mucosal tunic, there were three layers; ephitelial, propria, and muscularial mucous can be observed during the 14th day of incubation period. Ephitelial layer in the form of cylindrical epithelial cells with microvilli, proprial layer in the form of connective tissue, and layer of the muscularis mucosa form smooth muscle thread. In the mucosal tunic, there was Goblet cells and Liberkuhn crypts that can be observed during the 20th day incubation period and 7th day post hatch. Histomorphometry results of intestinal lining thickness, villi length, villi width, and lumen diameter of native chicken intestine during incubation and post hatching showed significant differences (P0.05). The findings of a strong positive reaction to the staining of PAS were found on the incubation period of the 20th day and post hatching the 7th day and positively weak on the incubation period of the 14th day. It can be concluded that there is a correlation between macroscopic development of chicken intestine, histomorphometric of tunica and villous thickness, as well as the distribution of strong positive reactions to the staining of PAS during the incubation period of the 20th day and post hatching the 7th day.
Evaluasi Validitas Human Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit dan Waktu Sentrifugasi Sampel Darah untuk Pengukuran Konsentrasi Hormon Kortisol pada Kambing Kacang Gholib Gholib; Sri Wahyuni; Rahma Melinda; Muslim Akmal
Jurnal Agripet Vol 21, No 1 (2021): Volume 21, No. 1, April 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i1.18153

Abstract

ABSTRAK. Penggunaan human cortisol enzyme-linked immunosorbent assay (ELISA) kit untuk pengukuran hormon kortisol pada hewan dan keterlambatan waktu sentrifugasi sampel darah untuk analisis hormon perlu dievaluasi. Penelitian ini bertujuan untuk mengevaluasi validitas human cortisol ELISA kit (EIA-1887, DRG Instruments GmbH, Germany) untuk pengukuran konsentrasi kortisol dan menguji pengaruh keterlambatan waktu sentrifugasi sampel darah terhadap stabilitas konsentrasi kortisol pada kambing kacang. Sampel darah dikoleksi dari delapan ekor kambing kacang. Uji validitas kit EIA-1887 dilakukan secara: a) analitik (uji parallelism, akurasi, dan presisi), dan b) biologis (pengukuran kortisol sebelum dan setelah transportasi). Uji keterlambatan waktu sentrifugasi terhadap stabilitas konsentrasi kortisol dilakukan dengan 5 perlakuan yaitu disentrifugasi kurang dari 1 jam (P1/kontrol), 6 jam (P6), 12 jam (P12), 18 jam (P18),dan 24 jam (P24) setelah darah dikoleksi. Data uji parallelism dianalisis dengan uji persamaan kemiringan, uji presisi dihitung % CV (coefficient variation) intra-assay dan inter-assay, uji akurasi dihitung % recovery, uji T untuk validasi biologis, dan uji ragam (One Way Anova) untuk pengaruh waktu sentrifugasi. Hasil uji parallelism menunjukkan kurva sampel kambing kacang sejajar/parallel dengan kurva standar kortisol. Akurasi kit EIA-1887 adalah 103,437,85%, dan % CV intra-assay dan inter-assay adalah 10%. Konsentrasi kortisol setelah transportasi secara signifikan lebih tinggi daripada sebelum transportasi (p0,05). Adanya penurunan secara nyata konsentrasi kortisol pada darah yang disentrifugasi 24 jam (P24) setelah koleksi (p0,05). Kesimpulan, human cortisol ELISA kit (EIA-1887) memiliki validitas yang baik secara analitik dan biologis untuk digunakan dalam pengukuran konsentrasi kortisol pada kambing kacang. Keterlambatan waktu sentrifugasi selama 24 jam berpengaruh terhadap konsentrasi kortisol. (Evaluation the Validity of Human Cortisol Enzyme-Linked Immunosorbent Assay (ELISA) Kit and Centrifugation Time of Blood Sample for Measuring the Concentration of Cortisol in Kacang Goats) ABSTRACT. The use of human cortisol ELISA kit for measuring cortisol in animals and delayed to blood centrifugation time for hormone measurement need to be evaluated. This study aimed to evaluate the validity of human cortisol ELISA kit (EIA-1887, DRG Instruments GmbH, Germany) for cortisol measurement and effect of delayed to blood centrifugation time on cortisol concentrations in kacang goats. Blood was collected from eight kacang goats. Validation test of EIA-1887 kit was performed through: a) analytical (parallelism, accuracy, and precision tests), and b) biological validations (measuring cortisol concentrations before and after transportation). Five treatments were performed to test delayed to centrifugation time: blood centrifuged at 1 h (control, P1), 6 h (P6), 12 h (P12), 18 h (P18), and 24 h (P24) after collection. Parallelism data were analyzed by slope equality test, precision and accuracy calculated by % CV of intra-and inter-assay, and % recovery, respectively. Data of biological validation and centrifugation time effects were analyzed by Student t-test, and one way ANOVA, respectively. Results of parallelism showed that serial dilution curve of kacang goat plasma was parallel with cortisol standard curves. Accuracy of EIA-1887 kit was 103.437.85%, and % CV of intra-and inter-assay were 10%. Concentration of cortisol after transportation was significantly higher than before transportation (p0.05). Concentration of cortisol was significantly decreased when blood was centrifuged at 24 h after collection (P0.05). In conclusion, human cortisol ELISA kit (EIA-1887) is a reliable assay for measuring cortisol in plasma of kacang goat. Delayed to blood centrifugation time affect cortisol concentrations.
Histologis Perkembangan Embrio Ayam pada Masa Inkubasi Satu sampai Tujuh Hari Fitriani Fitriani; Husmimi Husmimi; Dian Masyitha; Muslim Akmal
Jurnal Agripet Vol 21, No 1 (2021): Volume 21, No. 1, April 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i1.18449

Abstract

ABSTRAK. Penelitian ini bertujuan melihat perkembangan embrio ayam secara histologi pada waktu 1, 2, 3, 4, 5, dan 7 hari inkubasi. Sampel yang digunakan adalah telur fertil yang didapat dari teaching farm Fakultas Pertanian Jurusan Peternakan Universitas Syiah Kuala. Pengamatan secara makroskopis dilakukan pada setiap sampel embrio ayam, sementara pengamatan mikroskopis struktur embrio ayam dilakukan dengan pembuatan preparat histologi. Hasil penelitian ini menampilkan data perkembangan embrio ayam secara spesifik dari umur inkubasi hari ke-1 sampai hari ke-7. Secara makroskopik dan mikroskopis, gambaran umum perkembangan embrio ayam selaras dengan informasi tabel pertumbuhan embrio ayam. Secara histologis, gambaran perkembangan struktur embrio ayam baru dapat diamati dengan baik yaitu pada umur inkubasi hari ke-3 sampai hari ke-7. Dapat disimpulkan bahwa secara histologi perkembangan embrio ayam baru dapat diamati pada masa inkubasi hari ke-3. Penelitian ini juga dapat dijadikan sebagai bahan acuan dasar untuk penelitian lanjutan terutama terhadap organogenesis pada embrio ayam. (Histological evaluation on the development of chicken embryo during the incubation periodfrom 1st to 7th days) ABSTRACT. The aim of this study was to find out the development of chicken embryos at day 1st, 2nd, 3rd, 4th, 5th, and 7th of incubation by histological method. The samples used were the fertile eggs obtained from the teaching farm of the Faculty of Agriculture, Department of Animal Husbandry, Syiah Kuala University. Macroscopic observations were made on each embryos sample, while microscopic observations of the embryos structure were carried out by histological preparations procedure. The results of this study presented the specific data of chicken embryos development from day 1st to day 7th of incubation. The macroscopic and microscopic data showed the general description of chicken embryos development similar to the information on the chicken embryo growth table. The microscopic results showed the structure description of the chicken embryos can be well observed at day 3rd to the day 7th of incubation. It can be seen the development of chicken embryos can be observed by histology method on day 3rd of incubation. This research can also be used as a basic reference for further research, especially on organogenesis of chicken embryos.
Histologi, Histomorfometri, dan Histokimia Hati Ayam Buras (Gallus gallus domesticus) Selama Periode Sebelum dan Setelah Menetas Siswandy Siswandy; Erdiansyah Rahmi; Dian Masyitha; Fitriani Fitriani; Fadli A. Gani; Zuhrawaty Zuhrawaty; Muslim Akmal
Jurnal Agripet Vol 20, No 2 (2020): Volume 20, No. 2, Oktober 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i2.16011

Abstract

ABSTRAK. Hati adalah kelenjar terbesar yang terdapat di dalam tubuh dan dapat dianggap sebagai organ pusat dalam pemeliharaan suplai energi. Penelitian ini bertujuan untuk mengetahui histologi, histomorfometri, dan histokimia sebaran glikogen pada hati ayam buras sebelum dan sesudah menetas. Sampel penelitian dibagi menjadi empat kelompok tingkat umur berbeda dan masing-masing kelompok berjumlah enam hati ayam. Hasil penelitian menunjukkan perbedaan nyata (P0,05) pertumbuhan hati ayam buras secara makroskopis. Pada setiap perkembangan sudah terdapat hepatosit berbentuk poligon yang memiliki inti sel bulat di tengah hepatosit, terdapat juga vena central yang dilapisi sel endotel pipih selapis serta didapati sinusoid yang berada diantara hepatosit, dan juga ditemukan Kupffers cell hanya pada hati ayam setelah menetas hari ke-7. Hasil pengukuran diameter vena central hati ayam buras hari ke-7, hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 17,70 1,16 m; 60,32 2,22 m; 49,316 1,93 m; dan 129,11 7,43 m. Hasil histokimia sebaran glikogen pada hati ayam buras hari ke-14, hari ke- 20 sebelum menetas dan hari ke-7 setelah menetas berturut-turut adalah 1,44 0,93; 2,27 0,74; dan 2,77 0,15. Dapat disimpulkan, bahwa diameter vena central dan sebaran glikogen meningkat seiring pertumbuhan ayam buras.(Histological, histomorphometrical, and histochemical of liver on native chicken (Gallus gallus domesticus) during pre and posthatch period) ABSTRACT. The liver is the largest gland in the body and considered as a central organ in maintaining energy supplies. The study aimed to determine the histologic, histomorphometric and histochemical of glycogen distribution in the liver of native chicken during pre and posthatching period.. The study sample were divided into four different age groups and each group consisted of six chicken livers. The results showed a significant difference (P0,05) in native chicken liver growth macroscopically. In every development there are already polygonal hepatocytes that have a round cell nucleus in the middle of the hepatocytes, there is also a central vein that is coated with a layer of flat endothelial cells and also sinusoids that are between the hepatocytes, and kupffer's cells also found in chicken liver 7th day posthatching. The results measurements of the central vein diameter in liver native chickens on the 7th, 14th, 20th pre hatching period and 7th post hatching were 17.70 1.16 m; 60.32 2.22 m; 49.32 1.93 m; and 129.11 7,43 m; The measure results of the glycogen distribution in liver native chickens on the 14th, 20th pre hatching period and 7th post hatching were 1,44 0,93; 2,27 0,74; and 2,77 0,15. It can be concluded, that central vein diameter and glycogen distribution were increased by growth of native chickens.
Aplikasi Metode Emersi Fiksatif Berbeda terhadap Morfologi Histologi Testis dan Epididimis Kambing Lokal(Capra sp.) Fitriani Fitriani; Husmimi Husmimi; Muslim Akmal
Jurnal Agripet Vol 18, No 1 (2018): Volume 18, No. 1, April 2018
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v18i1.8848

Abstract

ABSTRAK. Penelitian ini bertujuan untuk melihat morfologi histologi testis dan epididymis kambing lokal(Capra sp.) dengan umur 1-1,5 tahun. Penelitian ini merupakan penelitian eksperimen laboratorik dengan menggunakan 6 testis dan 6 epididimis yang diambil secara acak dan masing-masing di fiksasi dalam fiksatif formalin dan neutral buffered formaline (NBF) 10% dengan waktu fiksasi 15 hari. Pembuatan dan pengamatan jaringan testis dan epididimis dilakukan di laboratorium histologi FKH Unsyiah. Pengamatan mikroskopis secara kualitatif yang diamati pada 10 tubulus testis dan epididimis. Hasil pengamatan dianalisis secara deskriptif. Secara mikroskopik, gambaran struktur jaringan testis dan epididimis kambing terlihat jelas. Ruang antar membran tubulus pada metode emersi fiksatif formalin masih terlihat longgar, sedangkan pada metode emersi fiksatif NBF terlihat padat. Pengerutan sel sangat terlihat pada fiksatif formalin dibandingkan fiksatif NB, namun autolisis sel terlihat tidak nyata pada kedua fiksatif tersebut. Secara umum, kondisi membran tubulus terlihat utuh pada testis dan epididimis dengan fiksatif formalin dan NBF, namun sedikit terlihat degenerasi hidropis pada ruang antar sel dalam tubulus seminiferus. Dari hasil penelitian disimpulkan bahwa perbedaan hasil pada preparat testis dan epididimis dengan menggunakan fiksatif berbeda, kemungkinan dapat dipengaruhi oleh jenis bahan fiksatif, ukuran dan struktur yang berbeda pada kedua organ. Pemilihan metode emersi fiksatif pada jaringan testis dan epididimis sangat penting untuk mempermudah pengamatan morfologi histologi.(Application of different fixative emersi methods for histological morphology of testicular and epididymal local goats (Capra sp.))ABSTRACT. This research was conducted to observe the histological morphology of testicular and epididymal tissues of local goats at the same age (1-1,5 years old). This research is laboratory experimental research using 6 testes and epididymis 6 taken randomly and each fixed in fixative neutral buffered formalin (nbf) and formaline 10% with a 15 day fixation time. Preparation and observation of the epididymis and testicle tissue is performed in the laboratory of histology FKH Unsyiah. Qualitative microscopic observations were observed in 10 testes and epididymal tubules. The observations analyzed descriptively. Microscopically, the picture of tissue structure of the testes and epididymis of kacang goats is evident.The intermediate space of the tubules in the formalin fixation emersy method still looks loose, whereas in NBF fixative emersy method looks solid. Cell shrinking is highly visible in formalin fixation compared with NBF fixation, but cellular autolysis appears to be invisible in both fixation methods. Generally, tubular membrane conditions are seen intact on the testes and epididymis with formalin and nbf fixation. Differences in results on testicular and epididymal preparations using different fixative materials, may be affected by different types of fixation, size and structure in both organs. The selection of a fixative emersi method on testicular tissue and epididiymis is essential to evaluate the histologic morphology.
Rasio Jenis Kelamin Kelahiran Anak Kambing Peranakan Ettawa (PE) Hasil Inseminasi Buatan Menggunakan Spermatozoa Swim Up Sariadi Sariadi; Dasrul Dasrul; Muslim Akmal
Jurnal Agripet Vol 14, No 2 (2014): Volume 14, No. 2, Oktober 2014
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v14i2.1890

Abstract

(The ratio of birth sex of ettawa crossbreed goat by artificial insemination using spermatozoa swim up)ABSTRACT. The aim of this research was to determine the ratio of birth sex of ettawa crossbreed goat by artificial insemination using spermatozoa swim up. This research was at Reproduction Laboratory Faculty of Veterinary medicine Syiah Kuala university and Livestock Group SMD, Bener Meriah Regency. Fifteen female goats with age 8-24 months where used in this study and divided into 3 treatment groups (P0, P1, P2) and 5 replications of each. The first group (P0) inseminated with sperm without swim up; P1 and P2 inseminated with sperm which swim up for 5 minutes (P1) and 10 minutes (P2). The result of conception rates observation and sex ratio of children born tabulated into tables. The data was analyzed by using analysis of variance (ANOVA) one way pattern followed by the multiple test Duncan. The results showed that the conception rate and birth sex ratio of male born had significantly different (P0,05). The segregation of sperm which swim up increased the conception rate and birth sex ratio of male born.
Does FOXP2 gene polymorphism affect the duration of orogastric tube use in moderate to late preterm neonates? A cross-sectional study in Indonesia Mauliza, Mauliza; Dimiati, Herlina; Akmal, Muslim; Imran, Imran
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.933

Abstract

Premature and low birth weight neonates often struggle with oral intake due to immaturity or respiratory distress. Forkhead box protein 2 gene (FOXP2) is predicted to influence oral feeding ability in newborns, but studies assessing the role of this gene in influencing oral feeding ability are limited. The aim of this study was to investigate the role of FOXP2 gene polymorphism, particularly single nucleotide polymorphism (SNP) rs17137124, on the duration of orogastric tube (OGT) use in moderate to late preterm neonates. A multi-center cross-sectional study was conducted in Lhokseumawe, Aceh, Indonesia, from September 2021 to August 2023, involving neonates with 32–36 weeks gestational age. The DNA samples were isolated from the saliva, amplified using polymerase chain reaction (PCR), and the FOXP2 gene was sequenced. The associations between FOXP2 gene polymorphisms and other plausible factors on the duration of OGT use were determined using Chi-squared test, Fisher’s exact test or Pearson correlation as appropriate. Preterm neonates with the CC genotype had the longest OGT use, averaging 3 days (39.1%). TT genotype preterm neonates required OGT use for 4, 5, and 6 days (each 33.3%), while CT genotype neonates predominantly required it for 4 days (41.7%). No significant association was found between FOXP2 genotypes and OGT use duration (p=0.233). Similarly, neonates with C allele required OGT use for 3 and 4 days, while those with T allele mostly required between 4 and 6 days, with no significant association (p=0.110). Analysis using dominant (p=0.109) and recessive models (p=0.481) also showed no significant associations with OGT use duration. However, the study found significant associations between delivery mode (p=0.002) and gestational age (p=0.001) with duration of OGT use in preterm neonates. This study highlights that FOXP2 polymorphisms have limited association with the duration of OGT use among preterm neonates.
Co-Authors Abdul Harris Abdullah, Mohd Agus Nashri Agik Suprayogi Al Azhar Al Azhar Amiruddin . Amiruddin A Amiruddin Amiruddin Andi Novita Arman Sayuti Arman Sayuti Arman Sayuti Aulanni'am, Aulanni'am Awaluddin Awaluddin Bagus Dwijayanti Bani, Ika I. Basuki B. Purnomo Boni Anggara budianto panjaitan Chintya Desfariza Cut Dahlia Iskandar Cut Nila Thasmi Cut Nila Thasmi Darmawi . Darnifayanti, Darnifayanti Dasrul Dasrul Dedy Syahrizal Denny Irmawati Dewi Ratna Sari Dian Masyitah, Dian Dian Masyitha Dian Masyitha Dwinna Aliza Dwinna Aliza Eka Meutia Sari Erdiansyah Rahmi Erwin Erwin Fadli A. Gani Farida Athaillah Ferdian, Riyan Fitriani Fitriani Fitriani Fitriani Fitriani Fitriani Fitriani Fitriani Gholib Gholib Gholib Gholib, Gholib Hafizuddin Hafizuddin Hamdan - Hamdan H Hamdan Hamdan Hamdani Budiman Hamdani H Hamny Sofyan Herlina Dimiati, Herlina Hermawaty Tarigan Husmimi Husmimi Husmimi Husmimi Husnurrizal . Idawati Nasution Idawati Nasution Imran Imran Indra Sitorus Joharsyah J Juli Melia Ketut Adnyane Mudite Listin Handayani M Nur Salim M Nur Salim M. Aris Widodo Mahdi Abrar Mauliza , Mauliza Mauliza, Mauliza Mohd. Agus Nashri Abdullah Morteza Almuthahhar MUHAMMAD AGIL Muhammad Hambal Muhammad Jalaluddin Muhammad Jalaluddin Muhammad Toras Mulyadi Adam Mulyadi Adam Mulyadi Adam, Mulyadi Mulyadi M Mustafa Sabri Nanda Yulian Syah Nazaruddin N Nirmala, Fadya Nora Maulina, Nora Nora Usrina Nuzul Asmilia Patrick Flaggellata Rahma Melinda Rahmandi r Ramadhan, Akmal Rastina Rastina Razali Daud Razali R Rina Aulia Barus Rinidar - Rinidar R Roslizawaty R Rusli - Sariadi Sariadi Satria Tanjung Siswandy Siswandy Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Sugito Sugito Sugito Sugito Sukma, Zahwa Tamara Sulaiman Yusuf Sultan Fadhilla Taqwa Suriadi S Susi Darmayanti Sutiman B. Sumitro Suwandi, Abdulhadi Syafruddin S Syafruddin S Syafruddin Syafruddin Syafruddin Syafruddin Syahrun Nur T. Armansyah T. Armansyah T. Armansyah, TR T. Reza Ferasyi Taufiq P. Nugraha, Taufiq P. Teuku Reza Ferasyi Tongku N Siregar Tongku Nizwan Siregar Tongku Nizwan Siregar Triva Murtina Lubis Triva Murtina Lubis Tuty Laswardi Yusuf Ummu Balqis Wahono Esthi Prasetyaningtyas Wahyu Sihombing Wahyuni, Sri Wibi Riawan Yuza Al Iqwal Zachreini, Indra Zainuddin Z Zainuddin, Zainuddin Zamzami, Rumi Sahara Zuhrawaty Zuhrawaty Zulkarnain Zulkarnain Zulkifli, Baidillah Zulyazaini Zulyazaini Zulyazaini, Zulyazaini