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All Journal Jurnal Sain Veteriner Buletin Veteriner Udayana Jurnal Veteriner Jurnal Kedokteran Brawijaya Media Kedokteran Hewan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Narra J Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Journal of Fisheries & Marine Jurnal Agro Veteriner

Poedji Hastutiek

Division Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 278480

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Detection of Goat Digestive Tract Protozoa Through Feces Examination in Kwanyar Sub-District, Bangkalan District Meyreta Doti Alcaterana; Poedji Hastutiek; Nunuk Dyah Retno Lastuti; Endang Suprihati; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence and species of protozoa that infect theÂ digestive tract of goats in Kwanyar Sub-District, Bangkalan District. This study was conducted in June-July 2019 with 100 samples of stool examination in the laboratory of the Division of Parasitology, Faculty of Veterinary Medicine, Airlangga University. On examination found two types of protozoa, which were: Eimeria sp and Blastocystis sp. The results of this study showed the prevalence of 48% digestive tract protozoa. The results of statistical analysis using the Chi-Square test showed significant differences in age under one year and over one year in goats (p <0.05).

Prevalence of Gastrointestinal Protozoa on Bali Cattle in Lopok Sub-District, Sumbawa District Aldi Hamdani; Nunuk Dyah Retno Lastuti; Yeni Dhamayanti; Setiawan Koesdarto; Agus Sunarso; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence of gastrointestinal protozoa on Bali cattle in Lopok Sub-District, Sumbawa District. The study was conducted from January to April 2021 by taking 100 samples of Bali cattle feces from 7 villages in Lopok Sub-District, Sumbawa District. Samples were examined by sedimentation and floating methods. Species identification was determined by the morphology of the protozoa. A total of 62 (62%) samples were positively infected with protozoa. There were 4 species of gastrointestinal protozoa found infecting Bali cattle, namely Eimeria sp., Blastocytis sp., Entamoeba sp., and Balantidium sp. Most of the protozoa found in this study were Eimeria sp. (54%) and Blastocytis sp. (5%). This study found 59 samples (59%) were infected by one type of protozoa, 3 samples (3%) were infected by two types of protozoa. Cattle aged 0-6 months (81%) had a higher prevalence rate and were more susceptible to protozoal infections than cows aged 7 months â€“ 2 years (70.3%) and more than 2 years (45.2%).

Ectoparasite Infestation on Beef Cattle (Bos Indicus) in Kendit Sub-District, Situbondo District Nizar Bachrudin Prihandono; Endang Suprihati; Lilik Maslachah; Poedji Hastutiek; mufasirin mufasirin
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to determine the type and percentage of ectoparasites attacking the cattle cow in the Kendit Sub-District, Situbondo District. The research was conducted from July to September 2020 with 100 samples of cow taken. The identification of ectoparasites was carried out at The Laboratory of the Animal Health Center of Situbondo Regency. This study was survey with a cross-sectional design. This study found several ectoparasites of the type of flies that infested the cattle cow: Musca domestica 29.20%, Haematobia irritans 58.96%, Stomoxys calcitrans 2.82%, Tabanus rubidus 0.42%, Tabanus megalops 0.56%, Hippobosca maculata 0.14%. In addition, this study also found ectoparasites of fleas that attacked the cattle cow, among others Bovicola bovis 2.12%, and Haematopinus eurysternus 5.78%. In this study, the dominant type of fly ectoparasite was Haematobia irritans 58.96% and the dominant ectoparasite from flea was Haematopinus eurysternus 5.78%. The suggestions for this research were this research should be continued with a longer time, the fishing method must be more varied because the more fishing methods used, the higher diversity results and it is advisable to maintain cage sanitation, improve maintenance management, and the cleanliness of cattle sheds surroundings to maintain cattle condition.

Identification and Prevalence of Blood Protozoa on Culling Layer Hen Sold in Wonokromo Traditional Market Surabaya City Moch. Rizky Darmawan; Poedji Hastutiek; Arimbi Arimbi; Sunaryo Hadi Warsito; Dian Ayu Permatasari; Ririn Ririn
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

The most common types of blood protozoa in chickens are from the phylum of Apicomplexa, including Plasmodium, Leucocytozoon and Haemoproteus. Blood protozoan diseases can cause decreased egg production, stunting and death in poultry farms. This study aims to identify and calculate the prevalence of blood protozoan infection in casted off laying chickens sold at Wonokromo Market, Surabaya City. One hundred blood samples from casted off laying chickens were taken by purposive sampling method. Blood samples were collected in Ethylene Diamine Tetra Acetate (EDTA) tubes, and then taken to the Parasitology Laboratory to make a blood smear which was then examined using a microscope with a magnification of 1000x. The results showed that the single infection was 43% positive for Plasmodium sp., 3% positive for Leucocytozoon sp. and 3% for mixed infections (Plasmodium sp. and Leucocytozoon sp.), with a total prevalence of positive samples infected with blood protozoa was 49%.

Identification of Ectoparasites and Gastrointestinal Parasites in Eurasian Tree Sparrow (Passer montanus) Moh. Ilham Rizkul Ulum; Mochamad Lazuardi; Nunuk Dyah Retno Lastuti; Poedji Hastutiek; Soeharsono Soeharsono; Amirul Muslim Amrullah
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence and identify ectoparasite and gastrointestinal parasites in Eurasian tree sparrow (Passer montanus) in Kediri Regency, East Java, Indonesia. The research was conducted in January 2021 with 100 samples of body swabs and feces of Eurasian tree sparrow. The examination was done in the Anugerah Satwa Veterinary Clinic in Kediri Regency using direct smear and flotation methods. The observations in this study showed that from 100 samples of body swabs, 38 samples (38%) were infested with ectoparasites which are Dermoglyphus sp. and Strelkoviacarus sp. and from 100 feces samples, 47 samples (47%) were infected with helminthic eggs with the single infections by Ascaridia sp. as much as 12%, Dispharynx sp. as much as 1% and mixed infections of Ascaridia sp. and Dispharynx sp. were 25%. Assessed by examination for the protozoa also found that Isospora sp. was present with prevalence of 100% of the 100 sparrow feces samples examined.

Prevalence of Helminthiasis in Cattle through Fecal Examination in Magetan Regency Firda Shafa Salsabila; I Komang Wiarsa Sardjana; Suryanie Sarudji; Budiarto Budiarto; Poedji Hastutiek; Ririn Ririn
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence of helminthiasis and find various kinds of egg worms in cattle through feces examination in Magetan Regency. A hundred cattle feces samples were collected from six animal markets in Magetan Regency. The Faecal examination was performed by native, sediments, and Flotation methods. The results of this study showed that there were 40 samples positively infected by a helminth, which indicated that the prevalence of helminthiasis in cattle was 40%. The kind of helminth were Oesophagostomum sp. (14%), Bunostomum sp. (8%), Mecistocirrus sp. (8%), Haemonchus sp. (4%), Toxocara vitulorum (3%), Trichuris sp. (2%), Strongyloides sp. (2%), and Fasciola sp. (11%).

Culling Layer Hen Ectoparasite Detection Sold at Wonokromo Market Surabaya City Nashihatus Sholihah; Sherbina Bai; Sri Hidanah; Dhandy Koesoemo Wardhana; Agus Sunarso; Poedji Hastutiek; M.Gandul Atik Yuliani
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of the research were to identify the species, prevalence, and predilection of ectoparasite in Culling Layer Hen at Wonokromo Market, Surabaya City. The research was conducted from March to May 2021 with total of 100 sample of chickens were examined by the skin and feather body region. Identification of ectoparasite was carried out at The Laboratory Parasitology, Faculty of Veterinary Medicine Universitas Airlangga using permanent mounting methods. The results showed, the species of ectoparasite that infest the culling layer hen were Menopon gallinae 21%, Lipeurus caponis 10%, Ornythonissus bursa 10%, and Dubininia melopsitacci 70%. Predilection of ectoparasite shows that M. gallinae were found in the head-neck, back, wings, and legs region. L. caponis was found in the head-neck, wings, and back region. O. bursa was found in the legs and tail region, and D. melopsitacci was found in the entire body of chicken, the head-neck, back, wings, abdomen, legs, and tail.

Identifikasi Molekuler Blastocystis sp. pada Monyet Ekor panjang di Taman Nasional Alas Purwo Edward Yonas Kristijanto; Wiwik Misaco Yuniarti; Mufasirin Mufasirin; Poedji Hastutiek; Endang Suprihati; Boedi Setiawan; Puput Ade Wahyuningtyas; Doohan Mahendra
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p05

Zoonotic diseases can be transmitted through close interactions between long-tailed monkeys. Blastocystis sp. is one of the parasites that can attack mammals and is most commonly found in the intestinal tract. This study aims to analyze the presence of infection and the phylogenetic tree of Blastocystis sp. in long-tailed monkeys in Alas Purwo National Park, Banyuwangi, East Java. Identification of Blastocystis sp. in this study using morphological and molecular methods. A total of 100 stools were examined microscopically using the floating method, showing that 61 samples were positive, followed by a PCR test with a target of 600bp. PCR results obtained three positive samples followed by squencing. Sequences processed in BLAST isolate samples TNAP2 and TNAP9 having homology with Blastocystis sp. Subtype 3 was 98-99%, while the TNAP7 isolate had a lower homology level of 78-79% and the level of phylogenetic analysis of the TNAP2 and TNAP9 isolates was related to Blastocystis sp. from the Philippines (KY610153.1) and Egypt (OP942294.1) and the TNAP7 isolate is related to Blastocystis sp. from Thailand (MH197670.1, MH197668.1). Isolate Blastocystis sp. from Alas Purwo National Park has high homology analysis results to Blastocystis type hominis from Rep. Czech and Chinese by 80-99%, it is possible to have a connection with the zoonotic problem.

Effect of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic cells and Leydig cells of gentamicin-induced rats Nailul Ngizzah; Wurlina Wurlina; Poedji Hastutiek; Iwan Sahrial Hamid; Eka Pramyrtha Hestianah; Lita Rakhma Yustinasari
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.99-106

This study aimed to determine the effect of the administration of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic and Leydig cells of gentamicin-induced rats (Rattus norvegicus). This study used 25 white male rats divided randomly into five groups. The rats were injected with Gentamicin 5 mg/kg bw/day subcutaneously for 14 days, except for the negative control group (NCG). During the next 14 days, the rats were given oral moringa leaf extract of 200, 316, and 500 mg/kg bw/day respectively for E200, E316, and E500 groups. The data were analyzed with one-way ANOVA followed by Duncan's test. The results showed that the number of spermatogonia, spermatocytes, and spermatids in NCG was higher (p <0.05) than in the other groups. The number of spermatogonia, spermatocytes, and spermatids in the E316 group was higher (p <0.05) than in PCG. The number of spermatogonia in the E500 group was higher (p <0.05) than in the E316 group, but the number of spermatocytes and spermatids in the E316 group was similar (p >0.05) to the E500 group. The number of PCG Leydig cells was the smallest (p <0.05) compared to the other groups. The administration of Moringa leaves extract in the E200 and E316 groups increased (p <0.05) the number of Leydig cells compared to PCG. The number of Leydig PCG cells in the E500 group was not significantly different (p >0.05) from that of the NCG. It could be concluded that administration of Moringa oleifera leaves extract restored the number of spermatogenic and Leydig cells of gentamicin-induced rats.

Gambaran Patologi Hepar Ikan Lele Dumbo (Clarias gariepinus) yang Diinfeksi Bakteri Edwardsiella tarda [Featuring Liver Pathology of Clarias gariepinus Infected By Edwardsiella tarda] Arimbi Arimbi; Poedji Hastutiek2; Ratu Meidiza
Jurnal Ilmiah Perikanan dan Kelautan Vol. 9 No. 1 (2017): Jurnal Ilmiah Perikanan dan Kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v9i1.7632

AbstrakPenelitian ini bertujuan untuk mengetahui perubahan kerusakan hepar dari Clarias gariepinus yang diinfeksi dengan Edwardsiella tarda. Dua puluh empat Clarias gariepinus dengan berat rata-rata 20-25 gram, ukuran 10-12 cm dibagi secara acak menjadi 4 kelompok perlakuan (P0, P1, P2, dan P3). Masing-masing perlakuan dilakukan enam kali pengulangan dengan dosis Edwarsiella tarda 3 x 106 CFU/ ml kecuali kontrol (P0). Pada pengamatan mikroskopis menunjukkan bahwa hepar telah mengalami pembengkakan dan pucat. Pengamatan histopatologi menggunakan mikroskop cahaya dengan 400 kali pembesaran. Metode penilaian Bernet's Scoring digunakan untuk menentukan adanya degenerasi, nekrosis, dan kongesti dan infiltrasi leukosit. Kruskal-Wallis digunakan untuk pengujian statistika diikuti dengan pengujian Mann-Whitney. Hasil penelitian menunjukkan bahwa terjadi perubahan pada hepar Clarias gariepinus. Kerusakan parah terjadi pada perlakuan P2. Hal ini disebabkan pada pengamatan histopatologi menunjukkan adanya lesi seperti nekrosis dengan pembengkakan sel dan meningkatnya infiltrasi eritrosit pada pembuluh darah dibandingkan dengan P1 dan P3. AbstractThis research aimed to know the changed damage of liver Clarias gariepinus were infected by Edwardsiella tarda. Twenty four of Clarias gariepinus with an average weight of 20-25 gram, size 10-12 cm were randomly divided into four groups of treatment (P0, P1, P2, and P3). Six repetitions each those are given with the dosage of Edwardsiella tarda 3x106 CFU/ml except P0 (control). At the macroscopic observed, show that the liver becomes swollen and pale. The histopathological features of hepar were examined under light microscope in 400 times magnification. Scoring method were using Bernet Scoring Method to examine the presence of degeneration, necrotic, congestion, and infiltration of leucocytes. Then, Kruskal-Wallis test followed with Mann-Whitney test of statistical analysis. The result showed a change in liver of Clarias gariepinus. The most severe damage occurred on P2. It causes in histopathology examination showed lesion such necrotic area with infiltration of inflammatory cells and there was an increased of erythrocyte infiltration in blood vessels compared with P1 and P3.

Co-Authors Abdul Samik Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Agnes Theresia Soelih Estoepangestie Agus Wijaya Agustono, Bodhi Al arif, Mohammad Anam Alasrorik, Muhammad Hizbulloh Aldi Hamdani Amelia Dwita Safitri Amirotul Azhimah Amirul Muslim Amrullah Ana Amaliah Anastasya, Jelita Arimbi Arimbi Arimbi Arimbi Arsin, Andi A. Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azzahra, Farah Nabila Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan Budiarto Budiarto Choirul Oktavian Setiyadin Nayiron Dadik Rahardjo, Dadik Dadik Raharjo David Mohamad Qadafi Dewi Mahartina Dhandy Koesoemo Wardhana Dhimar Maulud Dyahningrum Dian Ayu Permatasari Diyah Ayu Candra Djoko Legowo Doohan Mahendra DWI PUTRI RAHMAWATI Eduardus Bimo Aksono Herupradoto Edward Yonas Kristijanto Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Ellza Agatha Damayanti Elok Apriliawati Emy Koestanti Sabdoningrum Endang Suprihati Endang Suprihati ERMA SAFITRI Fadila Zikri Amanda Fakhryyah Maharani Deviyanti Fania Selfiannisa Febrina Dian Permatasari Firda Shafa Salsabila Firman Hadi Fanani Gandul Atik Yuliani Gemini Alam Ghifari Lutfi Fauzi Hana Eliyani Hani Plumeriastuti Hasanuddin Ishak Hasib, Abdullah Hayuning Nurrodhiya Heni Puspitasari Herry Agoes Hermadi Herupradoto, Eduardus Bimo Aksono Ihda Hanny, Khurun'In Fadia Indah Norma Triana Indasari, Elly Nur Ira Sari Yudaniayanti Isra Wahid Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Jihaan Haajidah Kartika Aditiya Amelia Kasman Kasman Khairullah, Aswin Rafif Kiki Amalia Rama Kurniawan, Muhammad 'Ahdi Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lianny Nangoi Lilik Maslachah Lilik Maslachah Lita Rakhma Yustinasari Loeki Enggar Fitri Lucia Tri Suwanti, Lucia Tri Marchelia Arifiandani Margaretha, Josephira Intan Mega, Toafan Satrio Melani Anggraini Meles, Dewa Ketut Meta Aprilia Meyreta Doti Alcaterana Mirni Lamid Moch. Rizky Darmawan Mochamad Lazuardi Moh. Ilham Rizkul Ulum Mohamad Safri Sauqi Mohammad Sukmanadi Moses, Ikechukwu Benjamin Mufasirin Muhammad Hambal Muhammad Hambal Muhammad Ridwan Nailul Ngizzah NASHIHATUS SHOLIHAH Nenny Harijani Nila Murodah Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nove Hidayati Nunuk Dyah Retno Lastuti Nusdianto Triakoso Panjaitan, Cahaya Permata Sari, Dian Ayu Permatasari, Dian Ayu Praja, Ratih Novita Pratama, Bima Putra Pratama, Ponasari Galuh Prawasa, Ramadhiniyanti Putri Alif Primarizky, Hardany Primarizky, Hardany Puput Ade Wahyuningtyas Rahaju Ernawati Rahmi Sugihartuti Ramadhanty, Miladhiyah Nabila Ratna Damayanti Ratu Meidiza Retno Bijanti Retno Wulansari Ridha, Muhammad R. Ridwan Amiruddin Ririn Ririn Riwu, Katty Hendriana Priscilia Rochmah Kurniasanti Rochmah Kurnijasanti Rohmah, Miftahur Rohmah, Siti Nur Sandy, Berliana Dwi Nandita Santoso, Kuncoro Puguh Saputro, Amung Logam Sardjana, I Komang Wiarsa Sari, Aulia Puspita Setiawan Koesdarto Sherbina Bai Soeharsono Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Mumpuni Sosiawati Sudirman Nasir Suherni Susilowati Suherni Susilowati Sulaeman, Nur Sa'adah Sunarso, Agus Sunaryo Hadi Warsito Suroiyah, Fifi Anik Suryanie Sarudji Talita Yuanda Reksa Tamaulina Br Sembiring Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Utami, Kinanti Putri Venti Safitri Warda Nafalizza Efendi Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widodo, Oky Setyo Wiwik Misaco Yuniarti Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad

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