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All Journal Jurnal Sain Veteriner Buletin Veteriner Udayana Jurnal Veteriner Jurnal Kedokteran Brawijaya Media Kedokteran Hewan JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Narra J Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Journal of Fisheries & Marine Jurnal Agro Veteriner

Poedji Hastutiek

Division Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 278480

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Detection of Blood Protozoa Infecting Broiler Chicken Farms in Tanjung Gunung Village, District Jombang Marchelia Arifiandani; Endang Suprihati; Wiwik Misaco Yuniarti; Nunuk Dyah Retno Lastuti; Poedji Hastutiek; Sunaryo Hadi Warsito
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Broiler chicken farms could be a part of potential source infection Leucocytozoonosis and Plasmodiosis disease due to the death of livestock. Broiler farms in Tanjung Gunung, District Jombang near the fields, water, and trees. This could be increase the population of vectors infection Leucocytozoonosis and Plasmodiosis. The main purpose of this researchis to detect Leucocytozoonosis and Plasmodiosis infections on broiler farms in Tnjung Gunung Village, District Jombang using 50 broiler chickens by Purposive Sampling of 2 bredeers. The result show rhat there are 6 positive samples infected Leucocytozoonosis, while Plasmodiosis infection could not be fond. Need cage sanitation to break life cycles of vector Leucocytozoonosis and Plasmodiosis.

Prevalence of Blood Protozoa Disease on Cattle and Buffalo in Moyo Hilir Sub-District, Sumbawa District West Nusa Tenggara Melani Anggraini; Hardany Primarizky; Mufasirin Mufasirin; Lucia Tri Suwanti; Poedji Hastutiek; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research was to determine the type and prevalence of blood protozoa disease on cattle and buffalo in Moyo Hilir sub-district, Sumbawa District, Wes Nusa Tenggara. This research was taken on july until December 2018. This research used a non-experimental method through an observation study. The samples of this research were made in thin blood smear using 20% Giemsa’s stain and all samples were from 200 blood of cattle and buffalo in Moyo Hilir sub-district, Sumbawa District, West Nusa Tenggara. The results showed that the prevalence of blood protozoa disease was 11.5 Based on the type of blood protozoa, the highest prevalence was Anaplasma sp. (11%), followed by Theileria sp. (0.5%), while Babesia sp. And Trypanosoma sp. Were not found. Based on the livestock types, the prevalence in buffalo was higher (17%)than cttle (6%). Based on sex the prevalence in female was higher (12.6%) than male (10.11%). Based on age of livestock, the prevalance of livestock with age > 2 years old was higher (13.33%) than livestock with age ≤ 2 years old (10.4%) based on the location of the village, blood protozoa disease were only found in Olatrawa village (17.85%) and Serading village (17.82%), while in Kakiang and Moyo village were not found. The conclusion of this research indicate that prevalence of blood protozoa disease was 11.5% and only Anaplasma sp. And Theileria sp. Were found.

The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro Amelia Dwita Safitri; Iwan Sahrial Hamid; Poedji Hastutiek; Setiawan Koesdarto; Rahmi Sugihartuti; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design. There were five treatments of physiological NaCl solution (K-), piperazine sitrate (K+), etanol extract of bitter leaf 0,35% (P1), etanol extract of bitter leaf 1,4% (P2), etanol extract of bitter leaf 4,2% (P3), and each treatment was done in four replications. This research used ten Ascaridia galli in each treatment for all replications. The observation and recording of dead Ascaridia galli was done at 2, 4, 6, 8, 10 and 12 hours. Ascaridia galli were declared dead if there was no movement when disturbed by anatomy tweezer and when dipped in slightly warm water (50°C). The obtained data was analyzed using ANAVA and continued with Duncan Multiple Range Test. The result of this research show that etanol extract of bitter leaf (Vernonia amygdalina) has anthelmintic effects against Ascaridia galli worm in Vitro. In the extract with 4,2% concentration, there is anthelmintic property that almost the same as Piperazine sitrate 10 mg/ml. the higher the concentration of extract, the higher the property of anthelmintic. In probit analysis show that EC50 achieved by concentration 2.093% with the low concentration of .002% and the highest concentration of 3.632%. LT50 of ethanol extract of bitter leaf was 0.35% at 10.323 hours, 1.4% at 9.800 hours, 4.2% at 7.864 hours and Piperazine sitrate 10 mg/ml at 9.013 hours.

The Effectiveness of Ethanol Extract of Red Betel Leaf (Piper crocatum) Againts Mortality of Boophilus microplus Larvae in Vitro Meta Aprilia; Poedji Hastutiek; Rochmah Kurnijasanti; Lucia Tri Suwanti; Mohammad Sukmanadi; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this study is to know the effectiveness of ethanol extract of red betel leaf (Piper crocatum) against mortality of Boophilus microplus Larvae in Vitro, as well as knowing lethal concentration 50 (LC50) and lethal concentration 90 (LC90). Method that used in the research was completely randomized design. There were five treatments and each treatment was done in five replications. This research used ten Boophilus microplus larvae in each treatment for all replication. The observation and recording of mortality was done at 1, 2, 3, 4, and 5 hours. Boophilus microplus larvae were declared dead if there was no movement. The obtained data was analyzed using ANOVA factorial. The result of this research show that ethanol extract of red betel leaf (Piper crocatum) has effectiveness against mortality of Boophlius microplus larvae in Vitro. In the extract 10% concentration, there is effectiveness that almost the same as Noticks®. The higher concentration of the extract, the higher the effectiveness against mortality lavae.In probit analysis show that LC50 achieved by concentration 5,4% or 2.700mg/50ml and LC90 achieved by concentration 11,3% or 5.650mg/50ml.

The Prevalence and Infection’s Degree of Gastrointestinal Worm of Local Chicken (Gallus domesticus) in Kramat Village, District of Bangkalan, Madura, East Java Indonesia Ellza Agatha Damayanti; Poedji Hastutiek; Agnes Theresia Soelih Estoepangestie; Nunuk Dyah Retno Lastuti; Kusnoto Kusnoto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The study was aimed to know the prevalence and level of gastrointestinal worm investation of local chicken (Gallus domesticus) in Kramat Village, district of Bangkalan, Madura East Java province. The samples used in the study were 100 faeces of kampung chicken. using sedimentation and floating method. Faeces samples were tested, than followed by microscopically test to identify the worm is species. The result showed that in 38 out of 100 samples could be indentified some worm species as followed : Capillaria sp.14 (36,8%), Railletina 7 (18,4%), Hymenolepis 16 (42,1%) and Heterakis gallinarum 1 (2,6%). Using Mann Whitney U-test could be differentiated the level of the infection with an average of 45.53 in cock, while in hen with an average of 55.47. There was no significancy (p>0,05) on the level of worm gastrointestinal infection of different sex of kampung chicken.

The Distribution of Goat Gastrointestinal Tractus Worm Egg at Rambon District of Nganjuk Regency Arum Puspitasari; Boedi Setiawan; Setiawan Koesdarto; Kusnoto Kusnoto; Soeharsono Soeharsono; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the distribution of worm tract infection in foat digestion in Sub-district of Prambon, Nganjuk Regency. This research was conducted in Agustus 2018-Januari 2019 with 108 samples of stool examination iin laboratory of Helmintologi Airlangga University Department of Parasitology, were examinated by native, sedimentation. Floatation techniques, and distribution of worm types of feces. The results showed that 66 samples were positive infected by gastrointestinal worm, its indicated that helminthiasis was 61,11%. On examination it was found some kind of worm eggs, which are: Oesophagustomum spp., Bunostomum spp., Haemonchus spp., Trichostrongylus spp., Strongyloides spp., Trichuris spp., Chabertia spp and Moniezia benedini. The results of study showed the most of worm distribution in feces is Haemonchus spp.

Identification of Blood Parasite on Sacrifical Cattle Slaughtered during Idul Adha 1438 H in Surabaya City and Sidoarjo Regency Dhimar Maulud Dyahningrum; Mufasirin Mufasirin; Nenny Harijani; Poedji Hastutiek; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research is to detect the presence of blood parasite that infects sacrificial cattle slaughtered during idul adha 1438 H in Surabaya City and Sidoarjo Regency. This research used 147 blood samples of sacrificial cattle and used two methods, those are thin blood smear stained with Giemsa 20% and Microhematocrit Centrifugation Technique. Based on the result of the examination using a microscope with 1000x magnification, there are positive samples infected with blood parasite, that consist of Babesia sp., and Anaplasma sp.

Prevalence and Nematode Infection Level on Gastrointestinal Tract at Horse (Equus caballus) in Bangkalan Madura Elok Apriliawati; Mufasirin Mufasirin; Wurlina Wurlina; Poedji Hastutiek; Lucia Tri Suwanti; Benjamin Christoffel Tehupuring
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study is to determine the prevalence, infection level, sex and age effect on the infection level of GIT Nematode parasite in a horse. The fecal samples analyzed using the methods of native, sedimentation, floatation, and worm eggs count per gram of feces. Sample examination found 54 positive infected gastrointestinal nematode parasite with prevalence rate of 87% (54 from 62). The prevalence of Trichonema sp., Strongylus sp., and Parascaris equorum are 37.1%, 16.1%, and 1.6%. There were also mixed infestation like Strongylus sp. and Trichonema sp; Strongylus sp. and Parascaris equorum; Trichonema sp. and Parascaris equorum with total prevalence 27.4%, 1.6%, and 3.2%. Sex and age of horse had a very significant and significant effect in prevalence and infection level of Nematode parasite.

Prevalence and Identification of Digestive Tract Protozoa in Goats in Labang District Bangkalan Regency Warda Nafalizza Efendi; Lucia Tri Suwanti; Abdul Samik; Poedji Hastutiek; Mufasirin Mufasirin; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence and to identify gastrointestinal protozoa in goats in Sub-district of Labang, Bangkalan Regency, Madura, Indonesia. The research was conducted in April - July 2019 with 100 samples of goat’s stool. Examination was done in the laboratory of Parasitology in Faculty of Veterinary Medicine, Airlangga University using direct smear, sedimentation and flotation methods. The result showed that goats in Labang, Bangkalan Regency were infected by protozoa Eimeria sp. and Blastocystis sp. and the prevalence was 84%. There were no significant differences in the age and sex of goats (p > 0.05).

Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique Firman Hadi Fanani; Kusnoto Kusnoto; Poedji Hastutiek; Setiawan Koesdarto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 Î¼g/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa.

Co-Authors Abdul Samik Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Agnes Theresia Soelih Estoepangestie Agus Wijaya Agustono, Bodhi Al arif, Mohammad Anam Alasrorik, Muhammad Hizbulloh Aldi Hamdani Amelia Dwita Safitri Amirotul Azhimah Amirul Muslim Amrullah Ana Amaliah Anastasya, Jelita Arimbi Arimbi Arimbi Arimbi Arsin, Andi A. Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azzahra, Farah Nabila Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan Budiarto Budiarto Choirul Oktavian Setiyadin Nayiron Dadik Rahardjo, Dadik Dadik Raharjo David Mohamad Qadafi Dewi Mahartina Dhandy Koesoemo Wardhana Dhimar Maulud Dyahningrum Dian Ayu Permatasari Diyah Ayu Candra Djoko Legowo Doohan Mahendra DWI PUTRI RAHMAWATI Eduardus Bimo Aksono Herupradoto Edward Yonas Kristijanto Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Ellza Agatha Damayanti Elok Apriliawati Emy Koestanti Sabdoningrum Endang Suprihati Endang Suprihati ERMA SAFITRI Fadila Zikri Amanda Fakhryyah Maharani Deviyanti Fania Selfiannisa Febrina Dian Permatasari Firda Shafa Salsabila Firman Hadi Fanani Gandul Atik Yuliani Gemini Alam Ghifari Lutfi Fauzi Hana Eliyani Hani Plumeriastuti Hasanuddin Ishak Hasib, Abdullah Hayuning Nurrodhiya Heni Puspitasari Herry Agoes Hermadi Herupradoto, Eduardus Bimo Aksono Ihda Hanny, Khurun'In Fadia Indah Norma Triana Indasari, Elly Nur Ira Sari Yudaniayanti Isra Wahid Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid Jihaan Haajidah Kartika Aditiya Amelia Kasman Kasman Khairullah, Aswin Rafif Kiki Amalia Rama Kurniawan, Muhammad 'Ahdi Kusnoto Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lianny Nangoi Lilik Maslachah Lilik Maslachah Lita Rakhma Yustinasari Loeki Enggar Fitri Lucia Tri Suwanti, Lucia Tri Marchelia Arifiandani Margaretha, Josephira Intan Mega, Toafan Satrio Melani Anggraini Meles, Dewa Ketut Meta Aprilia Meyreta Doti Alcaterana Mirni Lamid Moch. Rizky Darmawan Mochamad Lazuardi Moh. Ilham Rizkul Ulum Mohamad Safri Sauqi Mohammad Sukmanadi Moses, Ikechukwu Benjamin Mufasirin Muhammad Hambal Muhammad Hambal Muhammad Ridwan Nailul Ngizzah NASHIHATUS SHOLIHAH Nenny Harijani Nila Murodah Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nove Hidayati Nunuk Dyah Retno Lastuti Nusdianto Triakoso Panjaitan, Cahaya Permata Sari, Dian Ayu Permatasari, Dian Ayu Praja, Ratih Novita Pratama, Bima Putra Pratama, Ponasari Galuh Prawasa, Ramadhiniyanti Putri Alif Primarizky, Hardany Primarizky, Hardany Puput Ade Wahyuningtyas Rahaju Ernawati Rahmi Sugihartuti Ramadhanty, Miladhiyah Nabila Ratna Damayanti Ratu Meidiza Retno Bijanti Retno Wulansari Ridha, Muhammad R. Ridwan Amiruddin Ririn Ririn Riwu, Katty Hendriana Priscilia Rochmah Kurniasanti Rochmah Kurnijasanti Rohmah, Miftahur Rohmah, Siti Nur Sandy, Berliana Dwi Nandita Santoso, Kuncoro Puguh Saputro, Amung Logam Sardjana, I Komang Wiarsa Sari, Aulia Puspita Setiawan Koesdarto Sherbina Bai Soeharsono Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Mumpuni Sosiawati Sudirman Nasir Suherni Susilowati Suherni Susilowati Sulaeman, Nur Sa'adah Sunarso, Agus Sunaryo Hadi Warsito Suroiyah, Fifi Anik Suryanie Sarudji Talita Yuanda Reksa Tamaulina Br Sembiring Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Utami, Kinanti Putri Venti Safitri Warda Nafalizza Efendi Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widodo, Oky Setyo Wiwik Misaco Yuniarti Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad

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