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All Journal Media Kesehatan Masyarakat Indonesia Jurnal Natur Indonesia JURNAL SAINS DAN TEKNOLOGI INDONESIA Journal of Marine Research Medical Journal of Indonesia Indonesian Journal of Forestry Research Jurnal AgroBiogen Buletin Iptek Tanaman Pangan EDUKASIA MEDISAINS Bioeksperimen: Jurnal Penelitian Biologi Forum Geografi Jurnal Florea JOURNAL ONLINE OF PHYSICS BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Penelitian Karet Jurnal Pendidikan Biologi Science and Technology Indonesia Jurnal Geologi dan Sumberdaya Mineral (Journal of Geology and Mineral Resources) Jurnal Kesehatan Bakti Tunas Husada Metode : Jurnal Teknik Industri ANNALES BOGORIENSES Al-Madrasah: Jurnal Ilmiah Pendidikan Madrasah Ibtidaiyah Jurnal Medik Veteriner Media Keperawatan Indonesia Bioedusiana: Jurnal Pendidikan Biologi JURNAL Al-AZHAR INDONESIA SERI HUMANIORA Jurnal Riset Kesehatan Poltekkes Depkes Bandung International Journal for Educational and Vocational Studies Jurnal Kesehatan Karya Husada REiLA: Journal of Research and Innovation in Language Jurnal Metaedukasi : Jurnal Ilmiah Pendidikan Lahjah Arabiyah: Jurnal Bahasa Arab dan Pendidikan Bahasa Arab Journal of Earth and Marine Technology (JEMT) Euler : Jurnal Ilmiah Matematika, Sains dan Teknologi MAHESA : Malahayati Health Student Journal JoMMiT : Jurnal Multi Media dan IT Journal of Applied Sciences, Management and Engineering Technology (JASMET) Muhammadiyah Journal of Geriatric Indonesian Journal of Earth Sciences Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan QULUBANA: Jurnal Manajemen Dakwah At-Tajdid: Jurnal Ilmu Tarbiyah HEUTAGOGIA: Journal of Islamic Education Tanfidziya: Journal of Arabic Education Makara Journal of Science Jurnal Manajemen, Organisasi, Dan Bisnis Jurnal Abdi Kesehatan dan Kedokteran Investama : Jurnal Ekonomi dan Bisnis Jurnal Pengabdian Pada Masyarakat Jurnal Teknologi Informasi Jurnal Keperawatan Sasindo : Jurnal Pendidikan Bahasa dan Sastra Indonesia Transforma: Jurnal Manajemen Journal Economic Management and Business Kapuas Jurnal Pengabdian Masyarakat Malahayati International Journal of Nursing and Health Science Al-Wasatiyah: Journal of Religious Moderation Jurnal Agro Veteriner Jurnal Geosaintek

Suharsono Suharsono

Balai Penelitian Tanaman Kacang-kacangan dan Umbi-umbian, PO Box 66 Malang Telp. (0341) 801468

Author-ID : 389315

Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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Correlation of Learning Motivation with Self Regulated Learning at SMA Negeri 1 Tasikmalaya City Mustofa, Romy Faisal; Nabiila, Alyaa; Suharsono, Suharsono
International Journal for Educational and Vocational Studies Vol 1, No 6 (2019): October 2019
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/ijevs.v1i6.1750

Self-regulated learning is a constructive process that is active in setting learning goals. This ability should be possessed by every individual to become a successful learner. Another thing that students must have to be successful in the process and learning outcomes are learning motivation. This study aims to determine the correlation between learning motivation with self-regulated learning in grade X MIPA 9 SMA Negeri 1 Tasikmalaya City. This research was conducted in April 2019. The research method used was correlational with a population of all class X MIPA 9 students as many as 30 people. Samples were taken using a saturated sampling technique so that the entire population was sampled. The research instrument used in this study is the Motivated Strategies for Learning Questionnaire (MSLQ), which consists of 2 parts, namely motivational beliefs and self-regulated learning strategies. The data analysis technique used is the bivariate correlation regression test. Based on the results of the study, it can be concluded that there is a strong correlation (R = 0.761; R2 = 0.580) between learning motivation and self-regulated learning. The contribution of learning motivation to self-regulated learning is 58%.

Isolasi Gen Penyandi Peroksidase melalui Penapisan terhadap Pustaka Genom Kedelai Kultivar Slamet Suharsono, Suharsono; Rachman, Faisal; Astuti, Utut Widy
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Screening to genomic library of soybean cultivar Slamet in ë phage to isolate the gene encoding for peroxidase(per) was performed by two steps. cDNA of per gene from Arabidopsis thaliana labeled by alkalin phosphatasewas used as probe. The first screening was done by using 105 recombinant pfu (plaque forming unit). The secondscreening was carried out on 100 recombinant pfu isolated from the first screening. The result of second screeningshowed that all screened clones were supposed containing per gene. The ë phage resulted from second screeningwas infected into E. coli BM 25.8. Due to the Cre recombinase in E. coli BM 25.8, two lox P sites flanking the insertDNA of soybean created recombination. The recombination in loxP sites resulted the excision and formedrecombinant plasmid containing per gene of soybean. To multiply, the recombinant plasmid was introduced into E.coli DH5á. Analysis southern hibridization of recombinant plasmid cut by Bam HI showed that Bam HI 3.7 kbfragmen of S52121R clone, 5,3 kb of S2412R clone, 2,4 and 5,3 kb fragments of S2532R clone were supposed tocontain per genes.

Analisis Keragaman Genetik Temulawak (Curcuma xanthorrhiza Roxb.) Menggunakan Penanda Amplified Fragment Length Polymorphism (AFLP) damayanti, dini; tajuddin, teuku; purwoko, devit; zulaeha, siti; suharsono, suharsono
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Curcuma xanthorrhiza Roxb, which is well-known as Java turmeric, has been extensively used in pharmaceutical industries in Indonesia. In spite of this commercial value, the identity of this species is commonly mistaken from other similar orange rhizomes Curcuma. Correct identity of these species is vital in pharmaceutical industries. The objective of the study was to determine genetic diversity of 32 accession Curcuma xanthorrhiza Roxb. Genomic DNA was extracted from leaf using Sodium Dodesyl Sulphate (SDS) modification. Amplified fragment length polymorphism (AFLP) was carried out according to the protocol ofAFLPTM plant mapping kit and the final polymerase chain reaction (PCR) products were separated using The Agilent 2100 Bioanalyzer. The number of fragment produced by 12 pairs primer combination of AFLP ranged from 42 to 60 with an average of 52. Data obtained was analyzed by the NTSys program. From the AFLP amplification on 32 DNA samples, it was proven that the accession of Curcuma xanthorrhiza Roxb. had a high degree of diversity. Based on analysis of AFLP and unweighted pair group with arithme average (UPGMA) it was shown that the accession of Curcuma xanthorrhiza Roxb. could be grouped into two cluster at relative ecludian distance of 0.10 (10%). Cluster I for accession from Palembang, Pacitan and Ciamis 2. Cluster II for accession from Makale, Pontianak, Kulonprogo, Mataram, Boyolali, Salatiga, Sumberejo, Bali, P. Seram, Sentolo, Purworejo, Samas Bantul, Ciamis1, Blora, Semarang, Poso, Kalsesl, Tagari, Merapi Farm, Salakaria, NTB, Menoreh, Karang Anyar, Mangunan, Medan, Toraja, dan Solok.

ANALISIS MARKA MORFOLOGI DAN MOLEKULER SIFAT KETAHANAN KEDELAI TERHADAP INTENSITAS CAHAYA RENDAH Handayani, Titin; Sastrosumarjo, Sarsidi; Sopandie, Didy; Suharsono, Suharsono; Setiawan, Asep
Jurnal Sains dan Teknologi Indonesia Vol. 8 No. 1 (2006)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Availability of moleculer marker to identify the important agronomic character of plant is needed to accelerate selection activity of plant. Particularly for the complex quantitatively inherited traits - like e.g. shading tolerance of soybean - the use of such a technique will speed up the process to produce adapted genotypes.The objective of this research is to identify the linkage of molecular marker RAPD with character of shading tolerance. The morphological specific characters wich is corelated to shade tolerance is the number of productive branchs. The intensity 75% of artificial shading is optimal level for doing selection of soybean genotypes. The inheritance of shading tolerance of soybean was controlled by gene with full dominant or by two genes pairs with dominant and ressesive epistasis. There was no maternal effect in the inheritance to shade tolerance. Heritability value (0.45 – 0.54) indicated that the proportion variation caused by the genetic factors was moderate. Molecular analysis by using RAPD technique showed that UBC153, ROTH 480.01, and ROTH 480.03 primer have polymorphic band that can be used for inheritance study and linkage analysis. All polymorphisms segregated independently of each ather. Interval mapping with Mapmaker/QTL indicated that the location of the three QTLs on linkage group were at marker tolerance locus of Roth 480.01-8125 , Roth 480.03-1125, and UBC 153-19125 .

EFIKASI CENDAWAN ENTOMOPATOGENIK UNTUK MENGENDALIKAN ULAT BULU Prayogo, Yusmani; Marwoto, Marwoto; Suharsono, Suharsono
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3068

The efficacy of entomopthogenic fungus for Tussock Moth Caterpillars Control. The occurrence of Tussock Moth Caterpillar (TMC) out break reported in some areas in East Java at Probolinggo, Malang, Batu and other districts were worried. The high caterpillar population not only attacking mangos, cherry, and wild watercress, but also migrating to living house before pupation. The available control of these caterpillar is chemical pesticide. However, thechemical will kill the natural enemies and other beneficial insects. A laboratory experiment to study the efficacy of five entomopathogenic fungus against TMC and pupae was conducted at the Entomology Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) in April 2011. Our experiment was arranged using completely randomized design (CRD) with three replicates using five entomopthogenic fungus. The efficacy was determined by the mortality of caterpillar/larva and pupae infected by the fungus. Result showed that these five fungus had not effectively kill the caterpillar, as the dense setae in integument acted as a mechanical barrier to fungus infection. The pupal stage was more susceptible to fungus infection. It was found that Paecilomyces fumosoroseus and Lecanicillium lecanii fungus were more effective than Metarhizium anisopliae, Beauveria bassiana and Nomuraea rileyi repectively. These two fungus could kill 90% of pupae and also were easier to be cultured in a wide range of natural media. Therefore P. fumosoroseus and L. lecanii are potentially used as a biological control agent against TMC out break.Keywords: Entomopathogenic fungus, hairy caterpillar, incubation period, colonization

PENGARUH RADIASI SINAR GAMMA DAN ASAM FUSARAT UNTUK MENINGKATKAN KETAHANAN ABAKA (MUSA TEXTILIS NEE) TERHADAP FASARIURN OXYSPORUM Damayanti, Fitri; Suharsono, Suharsono; Mariska, Ika
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i4.3285

ABSTRACTEffect of gamma radiation and fusaric acid for resistance to wild Fusarium disease onabaca plant (Mum textillis Nee). The problem in abaca production is wilt disease infectioncaused by Fusarium oxysporzim. The resistant variety against the pathogen has not beenavailable yet. The disease resistance character of the species might be improved throughsomaclonal variation and in vitro selection. Different pure toxin of fusaric acid concentration(0, 15,30,45,60, and 75 mg/l) was used as component selection to get new hope numbers ofresistant abaca to wilt Fusarium disease through in vitro selection. Concentration of 45 mgllfusaric acid is lethal for abaca, so we used this concentration as dose of selection to selectshoots from irradiated calli. Gamma irradiation was used as mutagent to increase somaclonalvariation on abaca. Six levels of gamma-ray radiation (0, 0.5, 1, 2, and 3 Krad) were appliedto embriogenic calli. Increasing dose of radiation decreased the viability of calli. LDso wasfound between 1-1.5 Krad of radiation dose. In vitro selection was carried out in two stages.The concentration of selection of hsaric acid in stage I1 was increased one level to theconcentration in stage 1. Stage I selection of shoots from irradiated calli on mediumcontaining pure toxin 45 mg/l fusaric acid, showed that the survival capacity decreasing withthe increasing doses of gamma irradiation. In stage 11, shoots from irradiated calli (at 0.5 and1 Krad) could survive on medium containing 60 mg/l fusaric acid. In medium selectioncontaining 50% filtrate F. oxysporum, fusaric acid resistant shoots were also filtrate resistant.There was a correlation between in vitro fusaric acid and filtrate of F. oxysporum resistantplant and conidia suspension of F. oxysporum resistkt plant in the greenhouse.Keywords: Gamma radiation, in vitro selection, fusaric acid, Musa textilis, Fusariumoxysporum

Pemetaan Kerusakan Terumbu Karang Akibat Kandasnya Kapal Tongkang di Taman Nasional Karimunjawa, Jawa Tengah Munasik, Munasik; Helmi, Muhammad; Siringoringo, Rikoh Manogar; Suharsono, Suharsono
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

ABSTRAK: Pemetaan kerusakan terumbu karang akibat kandasnya kapal tongkang di P. Tengah dan di P. Cilik, Taman Nasional Karimunjawa, Jawa Tengah telah dilakukan 2-3 bulan setelah kejadian dengan cara mengukur luas area kerusakan dan menilai kondisi terumbu karang. Pengukuran area kerusakan menggunakan metode penginderaan jarak jauh berbasis Drone (UAV Drone/pesawat tanpa awak) dan penilaian kondisi ekosistem terumbu karang dengan menggunakan metode Underwater Photo Transect (UPT). Tujuan penelitian ini adalah untuk mengetahui kondisi dan kerusakan terumbu karang akibat kandasnya kapal tongkang di P. Cilik dan P. Tengah, Taman Nasional Karimunjawa. Hasil menunjukkan telah terjadi kerusakan fisik di lereng atas terumbu karang pada kedua pulau tersebut berupa karang mati dan pecahan karang. Luas kerusakan terumbu karang di P. Tengah (1.420,32 m2) lima kali lebih luas daripada luas kerusakan terumbu karang di P. Cilik (267,22 m2). Luasnya kerusakan terumbu karang di P. Tengah kemungkinan akibat perbedaan jumlah kapal tongkang yang kandas, waktu kandas dan dominasi karang yang mudah patah (fragile). Komunitas karang keras yang dominan di lereng terumbu P. Tengah adalah karang bercabang (CB), Acropora bercabang (ACB) dan karang lembaran/foliose (CF). Luasan kerusakan dan kondisi terumbu karang akibat kandasnya kapal tongkang ini dapat digunakan untuk pengelola kawasan konservasi dalam perencanaan perlindungan dan restorasi ekosistem terumbu karang di Taman Nasional KarimunjawaÂ Â ABSTRACT: Mapping damage to coral reefs due to the barges grounding in Cilik Island (P. Cilik) and Tengah Island (P. Tengah), Karimunjawa National Park, Central Java was carried out 2-3 months after the event by measuring the extent of damage and coral reefs assessment. Damage area measurement using Drone (UAV Drone) based remote sensing and reef ecosystem assessment using the Underwater Photo Transect (UPT) method. The aim of this study was to determine the condition and damage of coral reefs due to the wrecking of barges in Cilik and Central P., Karimunjawa National Park The results show that physical damage has occurred on the upper slopes of coral reefs on both islands is dead coral and coral fragments. Damage to extensive coral reefs in P. Tengah (1,420.32 m2) is five times larger than extensive damage to coral reefs in P. Cilik (267.22 m2). Damage to coral reefs in P. Tengah may cause differences in the number of barges that ran aground, time of aground and the dominance of fragile corals the upper slopes of the coral reefs. The dominant hard coral communities on the upper slopes of P. Tengah reefs are generally composed the fragile corals, such as branching corals (CB), branching Acropora (ACB) and foliose corals (CF). Damage to coral reefs and the condition of the ecosystem caused by the aground of the barges can be used to manage conservation areas in the planning of conservation and restoration of coral reef ecosystems in the Karimunjawa National Park in near future.

Groundwater quality in Ponjong Karst, Gunungkidul Regency, Special Region of Yogyakarta Sari, Avellyn Shintya; K, Sari Bahagiarti; Suharsono, Suharsono; Prasetyadi, C
Journal of Earth and Marine Technology (JEMT) Vol 1, No 1 (2020)
Publisher : Lembaga Penelititan dan Pengabdian kepada Masyarakat - Institut Teknologi Adhi Tama Suraba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31284/j.jemt.2020.v1i1.1142

The hydrogeological condition of a region is determined by the type of lithology, morphology, and subsurface conditions where the condition is very helpful in the study of groundwater exploration. Especially when we encounter interesting geological conditions when the groundwater exploration in the karst area in which volcanic rocks are resulting from the activities of the active volcano in the past. Groundwater exploration at Ponjong area and surrounding does have its special characteristics, because it has 2 different rock characteristics where the northern part of the research area in the form of a karst area (limestone of Wonosari Formation) while the southern part is an area of ancient volcanoes with dominant volcanic rocks from Wuni Formation, As for the research on the above and subsurface research area where the research is conducted based on surface geology mapping, geoelectric data collection, and groundwater sampling. Geological mapping includes geomorphological data, stratigraphy, and geological structure. The aquifer system of the research area can be divided into two systems, inter-grain aquifer systems, and fracture aquifer systems. Overall quality groundwater in the research area is quite good. Ponjong District there are mining activities that have resulted in changes in the land use system in the area. This can cause changes in the water surplus in the area due to the changing catchment area.

Hubungan Pengetahuan Dismenore terhadap Sikap Mengatasi Dismenore pada Mahasiswi Pendidikan Biologi Universitas Siliwangi Septiana, Erni; Suharsono, Suharsono; Putra, Rinaldi Rizal
MEDIA KESEHATAN MASYARAKAT INDONESIA Vol 19, No 6 (2020): MKMI
Publisher : Fakultas Kesehatan Masyarakat, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/mkmi.19.6.419-424

ABSTRAKLatar belakang: Penelitian ini bertujuan untuk mengetahui hubungan pengetahuan dismenore terhadap sikap mengatasi dismenore pada mahasiswi Pendidikan Biologi Universitas Siliwangi. Penelitian ini dilaksanakan pada bulan September 2018 sampai dengan bulan Agustus 2020 di Universitas Siliwangi. Dismenore merupakan salah satu gangguan menstruasi yang sering terjadi pada wanita. Mahasiswi Pendidikan Biologi memiliki pengetahuan dasar mengenai materi reproduksi khususnya menstruasi dan gangguannya, sehingga memiliki bekal pengetahuan tentang dismenore. Pengetahuan mahasiswi tentang dismenore dapat berpengaruh terhadap sikap mengatasi dismenore.Metode: Penelitian ini merupakan penelitian kuantitatif yang bersifat noneksperimental dengan metode korelasional. Populasi dalam penelitian ini adalah seluruh mahasiswi Pendidikan Biologi dari angkatan 2016 hingga 2018 sebanyak 299 orang. Sampel dalam penelitian ini adalah mahasiswi Pendidikan Biologi angkatan 2016 yang sudah mengontrak mata kuliah Anatomi dan Fisiologi Tubuh Manusia, sampel diambil menggunakan teknik purposive sampling. Data pengetahuan dismenore diperoleh melalui tes tertutup berupa tes pilihan majemuk 5 opsi sebanyak 16 soal, sedangkan data sikap mengatasi dismenore diperoleh melalui kuesioner. Pengujian hipotesis menggunakan analisis regresi linear sederhana.Hasil: Berdasarkan penelitian yang telah dilakukan, terdapat hubungan antara pengetahuan dismenore terhadap sikap mengatasi dismenore pada mahasiswi Pendidikan Biologi Universitas Siliwangi dengan nilai R sebesar 0,365 dan nilai R Square sebesar 0,133 yang berarti bahwa kontribusi yang diberikan dari aspek pengetahuan dismenore terhadap sikap mengatasi dismenore sebesar 13,3%.Kata Kunci: Dismenore, pengetahuan, sikapÂ ABSTRACTTitle: The Relationship between Knowledge of Dysmenorrhea and Attitudes to Overcome Dysmenorrhea in Biology Education Students at the University of SiliwangiBackgroud: This study aims to determine the relationship between knowledge of dysmenorrhea and attitudes to overcome dysmenorrhea in Biology Education students at the University of Siliwangi. This research was conducted from September 2018 to August 2020 at Siliwangi University. Dysmenorrhea is a menstrual disorder that often occurs in women. Biology Education students have basic knowledge of reproductive material, especially menstruation and its disorders, so they have knowledge of dysmenorrhea. Students' knowledge about dysmenorrhea can influence their attitude to overcome dysmenorrhea. Method: This research is a quantitative research which is non-experimental in nature with a correlational method. The population in this study were all Biology Education students from class 2016 to 2018 as many as 299 people. The sample in this study were students of Biology Education class 2016 who had contracted the Human Body Anatomy and Physiology course, the sample was taken using purposive sampling technique. Dysmenorrhea knowledge data obtained through a closed test in the form of a compound 5 option test as many as 16 questions, while the attitude to overcome dysmenorrhea data obtained through a questionnaire. Hypothesis testing uses simple linear regression analysis. Result: Based on the research that has been done, there is a relationship between knowledge of dysmenorrhea on attitudes to overcome dysmenorrhea in Biology Education students at the University of Siliwangi with an R value of 0.365 and an R Square value of 0.133 which means that the contribution given from the knowledge aspect of dysmenorrhea to attitudes to overcome dysmenorrhea is 13.3%.Keywords: Dysmenorrhea, knowledge, attitude

Resistance Analysis of CRISPR/Cas9 Genome-Edited Chili M2 Mutant Lines against Pepper Yellow Leaf Curl Viral Disease Prasetya, Wandy Murti; Hadiarto, Toto; Enggarini, Wening; Polosoro, Aqwin; Suharsono, Suharsono
Jurnal AgroBiogen Vol 17, No 1 (2021): JUNE
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n1.2020.p1-10

Pepper yellow leaf curl virus (PepYLCV) infection transmitted by silverleaf whitefly (Bemisia tabaci [Gennadius]) can decrease chili pepper yield up to 100%. At this moment, there is no chili pepper variety resistant to PepYLCV available. Genome editing approach through CRISPR/Cas9 is an effort to develop variety resistance to the viral infection. The purpose of this study was to obtain M2 lines developed by CRISPR/Cas9 system on proliferating cell nuclear antigen (PCNA) gene for resistance to PepYLCV. A total of four M2 lines (C47-7, L84-2, L84-23, and L120-19) consisting of 60 chili plants were tested for their resistance to PepYLCV. PCR analysis was performed to detect the presence (infection) of the virus. The results showed that a total of 35 plants derived from the four lines were resistant to PepYLCV. They consisted of 7 plants from C47-7 line, 11 plants from L84-2 line, 9 plants from L84-23 line, and 8 plants from L120-19 line. PCR analysis confirmed that the resistant plants obtained from this study were negatively infected by the virus. Since not all tested plants were resistant to virus infection, the PCNA gene allele in these resistant lines were most likely heterozigotes. Sequencing of PCNA gene of the resistant lines is needed to confirm that the resistance phenotypes obtained was due to mutation of the gene. Therefore, further selection needs to be performed to obtain stable and PepYLCV-resistant lines.

Co-Authors AA Sudharmawan, AA Adi Isworo, Adi Adikara, Tatang Santanu Agung Setiabudi Aini, Kuratul Akhmad Solikhin AKIHO YOKOTA Al arif, Mohammad Anam Al Firah, Al Firah ALEX HARTANA Alimuddin, Khasan Ana Quthratun Nada Angga Sugiarto Anik Herminingsih Apon Zaenal Mustopa Arif Rianto Budi Nugroho Aris Tjahjoleksono Ashadi Ashar N, Novema Aspariansyah, Aspariansyah Asrul Muhamad Fuad, Asrul Muhamad Astanto Kasno Avellyn Shinthya Sari Aviadi, Dedi Badriah, Liah Budianingsih, Budianingsih Cahyadi, Tedy Agung Caswita Caswita Cici Indriani Dalimunthe damayanti, dini Dani Ramdani, Dani Dessy Adelin Destiana, Destiana Didy Sopandie Diki Muhamad Chaidir Diponegoro, Muhammad Djaka Marwasta Eko Teguh Paripurno Elfa Cahya Jamiati Endang Surahman Erawati, Erna Faisal Rachman Febriana Dwi Wahyuni Feri, Zefki Okta Ferry Faisal, Ferry Firdaus, Syarifin Fitri Damayanti Fitri Damayanti Fitri Wibowo Fitri, Novi Aryani Gandhi Sutjahjo Gesa Cantya Primatara Gunawan, Heru Hadiarto, Toto Hamim Hamim Harijani, Nenny Hariyadi, Rahmat Hasan Labiqul Aqil Hermana, Salma Junaina Hidanah, Sri Hutabarat, Madya I, Heriberta Reny Idayati, Tutik Rosita Idha Susanti Iif Rahmat Fauzi, Iif Rahmat Ika Barokah Suryaningsih Ika Mariska IMAM MUSTOFA Irbah, Ariq Nurjannah Ishaq, Rizal Maulana Jayawarsa, A.A. Ketut Karuwal, Ritha Lusian KINYA AKASHI Komul, Marsha Krisdiana Wijayanti Kristanto, Paustinus Edi Kusuma, Amino V.A. Kusumayudha, Sari Bahagiarti Kuswanto, Edi Lulut Handayani Maemonah, Maemonah Mahmudah, Uswatun marwoto marwoto Mochamad Widjanarko Mufti Ali Muhammad Helmi Muhammad Jusuf Muhammad Sirril Wafa Muin, Muhammad Rusli Munasik Munasik Mustafawi, Wike Z. Mustofa, Romy Faisal Mustopa, Apon Z. Mutiara, Frista Mycelia Paradise Nabiila, Alyaa Nainggolan, Adinda Rachel Nasrul, Herni Widiyah Ningrum, Hanis Rachna Noorikhsan, Faisal Fadilla Novriyandi Hanif Nugroho, Arif Rianto Budi Nugroho, Arif Riyanto Budi Nuriyatul Fitriya Nurjanah, Fitri Nuryadin, Egi Oktavianti Oktavianti Palaniappan, Ananda Kumar Pausta Yugianus Pertiwi, Nadira Rasya Polosoro, Aqwin Pramono, Sidik Prasetya, Wandy Murti Prasetyadi, C Prasetyadi, Carolus Prasetyadi, Corolus Pratama, Yudha Agung Pujiastuti, Rr Sri Endang purwoko, devit RADITE TISTAMA RATIH DEWI HASTUTI Rikoh Manogar Siringoringo Rinaldi Rizal Putra, Rinaldi Rizal Rivai, Muhamad Rizal Rochadi Abdulhadi Rohananingsih, Rohananingsih Rondhianto Rondhianto S. Hidayat, S. Sahputra, Ngatno Sahroni, Sahroni Sahupala, Sapiah Samuel Agus Triyanto, Samuel Agus Santika, Wulan Puja Saputra, Muhammad Wahyu Saputri, Niken Anggraini Sri Sari Bahagiarti Kusumayudha Sari Bahagiarti Kusumayudha Sari, Avellyn Shintya Sari, Fatihah Fajar Sarmanu, Sarmanu Sarsidi Sastrosumarjo Septiana, Erni Setiahadiwibowo, Ajimas Pascaning Shohifaha , Salsabila Sirait, Budi Chrismanto Siti Zulaeha, Siti sodiq, alwi mahardhika Soekarta, Rendra Solly Aryza Sri Hidanah Sri Langgeng Ratnasari SRI RAHAYU Sugiyarto, Angga Sulistyaningsih, Yohana C Sulistyowati, Dina Indrati Dyah Supranianondo, Koesnoto Susanto, Abiyyu Naufal Sutarto Sutarto Sutrisno, Sutrisno Suyanta Suyanta Ta'adi, Ta'adi Teuku Tajuddin Tita Damayanti Lestari Titin Handayani Trisnowati, Yuni Dwi Trustinah Trustinah Ulfah Juniarti Siregar Utut Widy Astuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Vita Meylani Wahyu Hidayat Wamiliana Wamiliana Wendry Setiyadi Putranto Wening Enggarini Widya Paramita Lokapirnasari Widyastuti, Arum WIJAYANTO WIJAYANTO Winarso, Widyo Wiwik Misaco Yuniarti YanRiska Venata Sembiring, YanRiska Venata Yasir Arafat, Yasir Yeni Yulistanti Yohana Noradika Maharani Yuniati, Ratna YUSMANI PRAYOGO Zainal Muttaqin zulfitra, Zulfitra

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