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Identification of Drought-tolerant Local Cowpea Varieties of Southwest Maluku (Indonesia) Karuwal, Ritha Lusian; Suharsono, Suharsono; Tjahjoleksono, Aris; Hanif, Novriyandi
Makara Journal of Science Vol. 22, No. 4
Publisher : UI Scholars Hub

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Abstract

Cowpeas grown in Southwest Maluku have local potential due to their diversity and ability to adapt to drought stress conditions that otherwise cause low productivity. The purpose of this study was to identify of local cowpea varieties of Southwest Maluku that show tolerance to drought. The experimental samples were seven local varieties and threereference cultivars. The drought stress treatment was altered watering periods, i.e., every two (P0) and ten days (P1) from growth to harvest. The results of this research showed that a ten-day watering period significantly decreased plant height, number of leaves, number of root nodules, root and shoot dry weight, photosynthesis rate, stomatal conductance, transpiration rate, relative water content, media water content, chlorophyll content, and number and weight of seeds perplant. Drought stress treatment increased proline content and root length. Correlation analysis showed a significant correlation between plant height with all variables, except for root length, proline content, and weight of seedsper plant. The correlation and cluster analyses showed that the KM7 variety is a drought tolerant genotype among the local cowpea varieties from Southwest Maluku. Therefore, the KM7 variety can be used as plant material in future breeding programs.
ISOLASI DAN PENGKLONAN FRAGMEN cDNA DARI GEN PENYANDI MULTIDRUG RESISTANCE ASSOCIATED PROTEIN DARI Melastoma affine Suharsono, Suharsono; Firdaus, Syarifin; Suharsono, Utut Widyastuti
Makara Journal of Science Vol. 12, No. 2
Publisher : UI Scholars Hub

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Abstract

Isolation and Cloning of cDNA Fragment of Gene Encoding for Multidrug Resistance Associated Protein from M. affine. M. affine can grow well in acid soil with high level of soluble aluminum. One of the important proteins in the detoxifying xenobiotic stress including acid and Al stresses is a multidrug resistance associated protein (MRP) encoded by mrp gene. The objective of this research is to isolate and clone the cDNA fragment of MaMrp encoding MRP from M. affine. By reverse transcription, total cDNA had been synthesized from the total RNA as template. The fragment of cDNA MaMrp had been successfully isolated by PCR by using total cDNA as template and mrp primer designed from A. thaliana, yeast, and human. This fragment was successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5α. Nucleotide sequence analysis showed that the lenght of MaMrp fragment is 633 bp encoding 208 amino acids. Local alignment analysis based on nucleotide of mRNA showed that MaMrp fragment is 69% identical to AtMrp1 and 63% to AtMrp from A. thaliana. Based on deduced amino acid sequence, MaMRP is 84% identical to part of AtMRP13, 77% to AtMRP12, and 73% to AtMRP1 from A. thaliana respectively. Alignment analysis with AtMRP1 showed that MaMRP fragment is located in TM1 and NBF1 domains and has a specific amino acid sequence QCKAQLQNMEEE
ISOLASI DAN PENGKLONAN FRAGMEN cDNA DARI GEN PENYANDI MULTIDRUG RESISTANCE ASSOCIATED PROTEIN DARI Melastoma affine Suharsono, Suharsono; Firdaus, Syarifin; Suharsono, Utut Widyastuti
Makara Journal of Science Vol. 11, No. 2
Publisher : UI Scholars Hub

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Abstract

Isolation and Cloning of cDNA Fragment of Gene Encoding for Multidrug Resistance Associated Protein from M. affine. M. affine can grow well in acid soil with high level of soluble aluminum. One of the important proteins in the detoxifying xenobiotic stress including acid and Al stresses is a multidrug resistance associated protein (MRP) encoded by mrp gene. The objective of this research is to isolate and clone the cDNA fragment of MaMrp encoding MRP from M. affine. By reverse transcription, total cDNA had been synthesized from the total RNA as template. The fragment of cDNA MaMrp had been successfully isolated by PCR by using total cDNA as template and mrp primer designed from A. thaliana, yeast, and human. This fragment was successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5α. Nucleotide sequence analysis showed that the lenght of MaMrp fragment is 633 bp encoding 208 amino acids. Local alignment analysis based on nucleotide of mRNA showed that MaMrp fragment is 69% identical to AtMrp1 and 63% to AtMrp from A. thaliana. Based on deduced amino acid sequence, MaMRP is 84% identical to part of AtMRP13, 77% to AtMRP12, and 73% to AtMRP1 from A. thaliana respectively. Alignment analysis with AtMRP1 showed that MaMRP fragment is located in TM1 and NBF1 domains and has a specific amino acid sequence QCKAQLQNMEEE
ISOLATION, CLONING AND CHARACTERIZATION OF ACTIN-ENCODING cDNAs FROM Jatropha curcas L. IP-2P Yuniati, Ratna; Widyastuti, Utut; Sopandie, Didy; Yokota, Akiho; Akashi, Kinya; Suharsono, Suharsono
Makara Journal of Science Vol. 15, No. 2
Publisher : UI Scholars Hub

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Abstract

Actin is a major component of the plant cytoskeleton, so all cells contain this protein. Actin is expressed constitutively and is involved in basic housekeeping functions required for cell maintenance. Because of this, it has been frequently used as an internal control to normalize changes in gene expressions analysis. Actually, the information of nucleotide sequence of actin gene of Jatropha curcas L. population IP-2P from Indonesia is not available yet. The objective of this research was to isolate, clone and characterize cDNA of actin genes of J. curcas IP-2P. Three partial actin gene sequences had been successfully isolated by PCR using total cDNA as template, and actin primer designed from conserved region of Arabidopsis thaliana. Nucleotide sequence analysis showed that the length of JcACT fragment is 610, 534, and 701 bp encoding 203, 177, and 234 amino acids respectively. Local alignment analysis based on mRNA sequences shows that JcACT fragment shares 98% similarity with actin mRNA of Hevea brasiliensis and 99% with actin mRNA of Ricinus communis. Based on deduced amino acid sequence, JcACT is 100% identical to actins from Prunus salicina, Gossypium hirsutum, and Betula luminifera. Even though these clones of cDNA are not completed yet, they can be used as reference in J. curcas L. gene expression analysis.
ISOLASI DAN PENGKLONAN FRAGMEN cDNA DARI GEN PENYANDI MULTIDRUG RESISTANCE ASSOCIATED PROTEIN DARI Melastoma affine Suharsono, Suharsono; Firdaus, Syarifin; Suharsono, Utut Widyastuti
Makara Journal of Science Vol. 12, No. 2
Publisher : UI Scholars Hub

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Abstract

Isolation and Cloning of cDNA Fragment of Gene Encoding for Multidrug Resistance Associated Protein from M. affine. M. affine can grow well in acid soil with high level of soluble aluminum. One of the important proteins in the detoxifying xenobiotic stress including acid and Al stresses is a multidrug resistance associated protein (MRP) encoded by mrp gene. The objective of this research is to isolate and clone the cDNA fragment of MaMrp encoding MRP from M. affine. By reverse transcription, total cDNA had been synthesized from the total RNA as template. The fragment of cDNA MaMrp had been successfully isolated by PCR by using total cDNA as template and mrp primer designed from A. thaliana, yeast, and human. This fragment was successfully inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into E. coli DH5α. Nucleotide sequence analysis showed that the lenght of MaMrp fragment is 633 bp encoding 208 amino acids. Local alignment analysis based on nucleotide of mRNA showed that MaMrp fragment is 69% identical to AtMrp1 and 63% to AtMrp from A. thaliana. Based on deduced amino acid sequence, MaMRP is 84% identical to part of AtMRP13, 77% to AtMRP12, and 73% to AtMRP1 from A. thaliana respectively. Alignment analysis with AtMRP1 showed that MaMRP fragment is located in TM1 and NBF1 domains and has a specific amino acid sequence QCKAQLQNMEEE
Analysis of Groundwater Biological Parameters in Ponjong District, Gunung Kidul Regency, Special Region of Yogyakarta Sari, Avellyn Shinthya; Kusumayudha, Sari Bahagiarti; Suharsono, Suharsono; Prasetyadi, Corolus
Journal of Applied Sciences, Management and Engineering Technology Vol 4, No 2 (2023)
Publisher : Institut Teknologi Adhi Tama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31284/j.jasmet.2023.v4i2.5034

Abstract

This study shows that the condition of the well water tested based on physical, chemical and biological parameters to determine the feasibility of the water used by the surrounding community still meets the required quality standard thresholds. Although somewhat polluted because of the mining. Underground water in the karst area of Gunungkidul Regency is safe as raw water for drinking by residents and meets the standards set by the Indonesian Ministry of Health and Government Regulation Number 82 of 2001 concerning Water Quality Management and Water Pollution Control. If used as drinking water, it still has to go through water treatment or be heated to a certain boiling point, because it contains bacteria that may be harmful to humans.
Pengaruh Zakat Terhadap Sustainable Development Goals di Indonesia (Studi pada Seluruh Provinsi di Pulau Jawa) Muttaqin, Zainal; Suharsono, Suharsono; Alimuddin, Khasan
QULUBANA: Jurnal Manajemen Dakwah Vol. 4 No. 1 (2023): Qulubana: Jurnal Manajemen Dakwah
Publisher : STAI Terpadu Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54396/qlb.v4i1.939

Abstract

In its development, zakat has become one of the Islamic philanthropic institutions that work for the welfare of society and as an effort to assist in realizing the Sustainable Development Goals (SDGs) program. In fact, the contribution of zakat can not be said to be maximal to the SDGs in Indonesia. This is because the collection and distribution of zakat has not been optimal. This study aims to describe the effect of zakat on the SDGs in Indonesia (a study of all provinces on the island of Java). Of the 17 SDGs programs, only 5 programs were tested, namely (1) economic growth (GRDP), (2) poverty-free (JPM), (3) healthy and prosperous life, quality education, and decent work as measured by the Human Development Index ( HDI), (4) reduced inequality (GI), and (5) gender welfare (GPI). The method used is the Panel Vector Error Correction Model (PVECM). The results of this study prove that (1) Zakat in the short and long term has a negative and significant effect on GRDP, (2) Zakat in the short and long term has no effect on JPM, (3) Zakat in the short and long term has no effect on HDI, (4) Zakat in the short term has no effect on GI, but zakat in the long term has a positive and significant effect on GI, (5) Zakat in the short and long term has no effect on GPI.
Praktik Baik dalam Pengajaran Tata Bahasa BIPA Suharsono, Suharsono
Sasindo : Jurnal Pendidikan Bahasa dan Sastra Indonesia Vol 12, No 1 (2024): Januari 2024
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/sasindo.v12i1.18418

Abstract

Salah satu kompetensi yang perlu dikuasai oleh pemelajar BIPA dalam belajar bahasa Indonesia adalah kompetensi linguistik. Kompetensi linguistik adalah kemampuan untuk menggunakan kode linguistis secara akurat. Salah satu kemampuan yang perlu dikuasai dalam kompetensi linguistis adalah kompetensi yang berhubungan dengan gramatikal atau tata bahasa. Kemampuan gramatikal atau tata bahasa ini sering dipandang oleh kebanyakan pemelajar BIPA sebagai kemampuan yang lebih sulit dikuasai dibandingkan dengan kemampuan yang lain, seperti membaca, berbicara, menulis, dan menyimak, padahal kemampuan dalam tata bahasa menjadi dasar bagi kemampuan empat keterampilan berbahasa tadi. Begitu juga bagi pengajar BIPA, tata bahasa sering dianggap sebagai bidang yang lebih sulit diajarkan daripada empat keterampilan berbahasa. Tidak jarang pengajar BIPA menghindari penjelasan yang berkaitan dengan aspek ketatabahasaan apalagi jika yang dihadapi adalah pemelajar BIPA yang berada pada tingkat (level) lanjut. Makalah ini hendak menyajikan praktik baik dalam pengajaran tata bahasa BIPA. Diharapkan bahasan ini dapat memberikan wawasan dan inspirasi bagi pengajar BIPA yang mengajarkan tata bahasa atau yang karena tugasnya harus menjelaskan aspek-aspek ketatabahasaan BIPA. Yang dimaksud dengan praktik baik di sini adalah bagaimana cara memahamkan konsep tata bahasa bahasa Indonesia kepada pemelajar BIPA dengan mudah. Pembahasannya akan ditinjau dari segi sifat linearitas bahasa, sifat bahasa yang berpola, konteks, dan teknik penyajian
PENGARUH GARANSI PRODUK DAN KUALITAS PELAYANAN JASA TERHADAP KEPUTUSAN PEMBELIAN PRODUK ELEKTRONIK PADA PT.BHAKTI IDOLA TAMA Suharsono, Suharsono; Sahputra, Ngatno; Al Firah, Al Firah
Journal Economic Management and Business Vol 2, No 2 (2023)
Publisher : Universitas Dharmawangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46576/jfeb.v2i2.4177

Abstract

Penelitian ini bertujuan untuk menganalisis apakah garansi produk dan kualitas pelayanan jasa berpengaruh secara simultan terhadap keputusan pembelian produk elektronik PT. Bhakti Idola Tama, menganalisis apakah variabel garansi berpengaruh signifikan terhadap keputusan pembelian, dan menganalisis apakah variabel kualitas pelayanan jasa berpengaruh signifikan terhadap keputusan pembelian. Metode penelitian yang digunakan adalah kuantitatif. Pengumpulan data melalui kuesioner dananalisisdatamenggunakanujiregresi linier berganda. Hasil penelitian menunjukkanbahwa variabel garansi produk secara parsial berpengaruh tidak signifikan terhadap keputusan pembelian berdasarkan analisis uji t diperoleh nilai thitung< ttabel sebesar 1,565< 1,65 berarti jika variabel garansi produk ditingkatkan sebesar satu-satuan maka keputusan pembelian naik sebesar 0,116.Variabel kualitas pelayanan jasa secara parsial berpengaruh signifikan terhadap keputusan pembelian dengan nilai thitung> ttabel sebesar 8,105> 1,65 berarti H0 ditolak dan Ha diterima, artinya jika variabel kualitas pelayanan jasa ditingkatkan sebesar satu-satuan maka keputusan pembelian naik sebesar 0.594. Pada hasil uji F menghasilkan nilai Fhitung> Ftabelsebesar 51,616 >3,08 berarti H0 ditolak dan Ha diterima,artinya jika uji F variabel bebas yaitu garansi produk dan kualitas pelayanan jasa secara simultan dan signifikan berpengaruh terhadap keputusan pembelian produk elektronik pada PT. Bhakti Idola Tama. Nilai R Square = 0.486 berarti garansi produk dan kualitas pelayanan jasa mempengaruhi keputusan pembelian sebesar 48,6%. Sedangkan sisanya 51,4% dipengaruhi oleh variabel lain yang tidak termasuk dalam penelitian ini.
Peran Guru Agama dalam Penanaman Nilai-Nilai Multikultural di Sekolah Dasar Suharsono, Suharsono
Al-Madrasah: Jurnal Ilmiah Pendidikan Madrasah Ibtidaiyah Al-Madrasah Vol. 8, No. 1 (Januari 2024)
Publisher : Sekolah Tinggi Ilmu Al-Qur'an (SIQ) Amuntai Kalimantan Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35931/am.v8i1.3160

Abstract

Pendekatan multikultural dalam pendidikan Islam bertujuan untuk mengeksplorasi dan menghargai perbedaan, serta mempromosikan pemahaman, toleransi, dan kerja sama antar umat beragama dalam konteks sosial melalui peran guru Agama. Penelitian ini menggunakan pendekatan kualitatif dengan jenis penelitian studi kasus. Adapun metode pengumpulan data menggunakan metode observasi, interview serta dokumentasi. Teknik analisis data merujuk pada teori Miles dan Huberman dengan tahapan pengumpulan data, reduksi data, display data dan melakukan penarikan kesimpulan, Teknik pengecekan keabsahan temuan dilakukan dengan Teknik triangulas, member check, perpanjangan keikutsertaan, diskusi dengan teman. Hasil penelitian ini menunjukkan bahwa konsep yang dikembangkan melalui kegiatan di dalam kelas tersusun dalam RPP yang di dalamnya terdapat nilai toleransi dan saling menghormati, untuk kegiatan pembelajaran diluar kelas terkonsep dalam rapat mingguan, rapat bulanan dan tahunan. Proses pelaksanaan kegiatan di dalam kelas dengan metode ceramah dan tanya jawab, kegiatan diluar kelas melalui upacara bendera dan rutinitas keagamaan. Hasil penanaman nilai-nilai multikultural melalui kegiatan di dalam kelas adalah toleransi yang tercermin dalam sikap menghormati dan menghargai perbedaan keyakinan, persamaan hak dalam berbagai kegiatan di dalam kelas, melalui kegiatan diluar kelas kerukunan antar siswa muslim dan nasrani, saling bekerja sama dalam berbagai kegiatan, pembentukan identitas agama yang kuat.
Co-Authors AA Sudharmawan, AA Adikara, Tatang Santanu Agung Setiabudi Agung Setiabudi, Agung Aini, Kuratul Akhmad Solikhin AKIHO YOKOTA Al arif, Mohammad Anam Al Firah, Al Firah ALEX HARTANA Alimuddin, Khasan Ana Quthratun Nada Anik Herminingsih Apon Zaenal Mustopa Arif Rianto Budi Nugroho Aris Tjahjoleksono Ashadi Aspariansyah, Aspariansyah Asrul Muhamad Fuad, Asrul Muhamad Astanto Kasno Avellyn Shinthya Sari Aviadi, Dedi Badriah, Liah Baehaqi Bambang Yulianto Budianingsih, Budianingsih Cahyadi, Tedy Agung Caswita Caswita Cici Indriani Dalimunthe damayanti, dini Dani Ramdani, Dani Dessy Adelin Destiana, Destiana Didy Sopandie Diki Muhamad Chaidir Dina Indrati Dyah Sulistyowati Diponegoro, Muhammad Djaka Marwasta Egi, Egi Nuryadin Eko Teguh Paripurno Elfa Cahya Jamiati Endang Surahman Ernanda, Melania Faisal Rachman Febriana Dwi Wahyuni Feri, Zefki Okta Ferry Faisal, Ferry Firdaus, Syarifin Fitri Damayanti Fitri Damayanti Fitri Wibowo Fitri, Novi Aryani Fitriya, Nuriyatul Gandhi Sutjahjo Gesa Cantya Primatara Gunawan, Heru Hadiarto, Toto Hamim Hamim Hariyadi, Rahmat Hasan Labiqul Aqil Hermana, Salma Junaina Hidanah, Sri Hutabarat, Madya Idayati, Tutik Rosita Idha Susanti Iif Rahmat Fauzi, Iif Rahmat Ika Mariska IMAM MUSTOFA Irbah, Ariq Nurjannah Ishaq, Rizal Maulana Jayawarsa, A.A. Ketut Kagoya, Tundani Kartawan, Kartawan Karuwal, Ritha Lusian KINYA AKASHI Kristanto, Paustinus Edi Kusuma, Amino V.A. Kusumayudha, Sari Bahagiarti Kuswanto, Edi Lulut Dwi Sulistyaningsih Maemonah, Maemonah Mahmudah, Uswatun marwoto marwoto Miftahudin . Mochamad Widjanarko Muhammad Helmi Muhammad Jusuf Muhammad Sirril Wafa Muin, Muhammad Rusli Munasik Munasik Mustafawi, Wike Z. Mustofa, Romy Faisal Mustopa, Apon Z. Mutiara, Frista Mycelia Paradise Nabiila, Alyaa Nainggolan, Adinda Rachel Nasrul, Herni Widiyah Nenny Harijani Ningrum, Hanis Rachna Noka, Isara Abda Noorikhsan, Faisal Fadilla Novriyandi Hanif Nugroho, Arif Rianto Budi Nugroho, Arif Riyanto Budi Nuriyatul Fitriya Nurjanah, Fitri Nuryadin, Egi Oktavianti Oktavianti Palaniappan, Ananda Kumar Pausta Yugianus Pertiwi, Nadira Rasya Polosoro, Aqwin Prasetya, Wandy Murti Prasetyadi, C Prasetyadi, Carolus Prasetyadi, Corolus Pratama, Yudha Agung purwoko, devit RADITE TISTAMA Rasyidina, Sumayya Ainur RATIH DEWI HASTUTI Rikoh Manogar Siringoringo Rinaldi Rizal Putra, Rinaldi Rizal Rivai, Muhamad Rizal Rochadi Abdulhadi S. Hidayat, S. Safari, Neng Ayu Mustika Sahputra, Ngatno Sahroni, Sahroni Samuel Agus Triyanto, Samuel Agus Santika, Wulan Puja Saputra, Muhammad Wahyu Sari Bahagiarti Kusumayudha Sari Bahagiarti Kusumayudha Sari, Avellyn Shintya Sari, Fatihah Fajar Sarmanu, Sarmanu Sarsidi Sastrosumarjo Septiana, Erni Setiahadiwibowo, Ajimas Pascaning Shohifaha , Salsabila Sholihin, Achmad Sirait, Budi Chrismanto Sirojuddin, Wildan Siswahyudianto Siti Zulaeha, Siti sodiq, alwi mahardhika Soekarta, Rendra Solly Aryza Sri Hidanah Sri Langgeng Ratnasari SRI RAHAYU Sulistiani, Erina Sulistyaningsih, Yohana C Supena, Ence Darmo Jaya Supranianondo, Koesnoto Sutarto Sutarto Sutrisno, Sutrisno Teuku Tajuddin Tita Damayanti Lestari Titik Sudartinah Titin Handayani Trisnaningrum, Niken Trisnowati, Yuni Dwi Trustinah Trustinah Ulfah Juniarti Siregar Utut Widy Astuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Vita Meylani Wahyu Hidayat Wamiliana Wamiliana Wendry Setiyadi Putranto Wening Enggarini Widya Paramita Lokapirnasari WIJAYANTO WIJAYANTO Winarso, Widyo Wiwik Misaco Yuniarti YanRiska Venata Sembiring, YanRiska Venata Yohana Noradika Maharani Yulianto, Bernawan Yuniati, Ratna YUSMANI PRAYOGO Zainal Muttaqin zulfitra, Zulfitra