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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Manajemen dan Agribisnis Vegetalika Zuriat Jurnal Floratek Jurnal Keteknikan Pertanian Jurnal Agro Teknologi Tropika Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Plasma Nutfah E-Journal Widya Kesehatan dan Lingkungan AGRIVITA, Journal of Agricultural Science SC (Savana Cendana) : Jurnal Pertanian Konservasi Lahan Kering Jurnal Penelitian Pertanian Terapan Jurnal AGROSAINS dan TEKNOLOGI Caraka Tani: Journal of Sustainable Agriculture Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Biologi Tropis Jurnal Pertanian Agros Indonesian Food Science and TechnologyJournal Jurnal Penelitian Kelapa Sawit Jurnal Riset Akuakultur Journal of Tropical Crop Science Islamic Management: Jurnal Manajemen Pendidikan Islam Molekul: Jurnal Ilmiah Kimia Jurnal Agronomi Tanaman Tropika (JUATIKA) Agrotechnology Research Journal Jurnal Agroplasma Akta Agrosia Rayah Al Islam : Jurnal Ilmu Islam Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Penelitian Tanaman Industri (Littri) PAKAR Pendidikan Jurnal Agrotropika Agroteksos Journal of Applied Agricultural Science and Technology PERTANIAN TROPIK Jurnal Pendidikan Islam Jurnal Kesehatan Komunitas Indonesia Agrotech Journal
Muhamad Syukur
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Jurnal AgroBiogen

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Nucleotide Variations of Gamma Ray-Irradiated Chili Pepper Mutant Genotypes Based on Gene-Specific Primers Related to Fruit Size Character Nugroho, Kristianto; Trikoesoemaningtyas, Trikoesoemaningtyas; Syukur, Muhamad; Lestari, Puji
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p83-94

Abstract

Fruit size is a quantitative character controlled by several genes and mutations in those genes could lead to phenotypic changes in fruits. Single nucleotide polymorphism (SNP) is a molecular marker that could be used to detect mutation in genes at the nucleotide level that could not be done by conventional markers. The aim of this study was to analyze nucleotide variations, primarily SNP, in mutant chili pepper genotypes using gene-specific primers related to fruit size characters to measure the genetic changes occured, identify the SNPs that were associated with fruit size character, and analyze the phylogenetic among the mutant genotypes. Sanger sequencing was conducted on six mutant[K21]  chili pepper genotypes from M2 generation and Lingga as origin variety using five pairs of newly designed gene-specific primers corresponding to fruit size characters. A total of 41 base changes were identified in the gamma ray-irradiated chili pepper mutants, consisting of 22 insertions/deletions (indels) and 19 SNPs. As many as 17 nonsynonymous SNPs which caused amino acid changes, in contrast to the other 2 synonymous SNPs, were found in these mutant genotypes. Association analysis between identified SNPs to fruit length and diameter showed nonsignificant association, therefore the functional marker related to targeted gene could not be developed. The genotype L269 showed a separation from Lingga in dendrogram reflecting a large number of genetic changes, showed its potential as a new germplasm in development of improved variety in the future.
Co-Authors , Deviona, , , Misnen , Widodo , Yudiwanti , Yustiana ,, Rosminah ,, Sopiana ,, Usman . Kusmanto . Rustam . Sobir . UNDANG . Widodo Abdul Hakim Abdul Hakim Abdul Rahman Fajar Sidiq Abdulah Bin Arif Abdullah B. Arif Abdullah Bin Arif Abdullah Bin Arif Abdullah bin Arif Adriwan Siregar, Heri Ady Daryanto Agustina, Ferra Anggita Ahmad Fahrori Al Hasani Akbar Hidayatullah Zaini Akbar Hidayatullah Zaini Ali Djamhuri Amaliah, Wenny Anas Dinurrohman Susila Andi Takdir Makkulawu Andri Kusmayadi Anung Wahyudi Aprilianti, Devi Kurnia Arief Riza Wijaya, Arief Riza Arif Imam Suroso Arum Sholikhah Arya Yuda Pangestu Asep Setiawan Asril Siregar Awang Maharijaya Azahra, Salsabila Azis Rifianto Azmi, Tubagus Kiki Kawakibi Azmi, Yudia Bagas Akmala Putra Bambang S. Purwoko Bambang Sapta Purwoko Bintang, dan Maria Carolina Evelyn Chesaria, Nanda Constantin, Mondjeli Cucu Gunarsih Darmawan Asta Kusumah Dasumiati Desta Wirnas Dewa Ngurah Suprapta Dewi Sukma Diah Setiowati, Retno Dian Rakhmad Didik Ariyanto Didy Sopandie Dimas Purwo Anggoro Dinar Tri Soelistyowati Diny Dinarti Djayadiningrat, Muhamad Hyar Dwi Guntoro Eka Martha Della Rahayu Elza Zuhry Endah Retno Palupi Endang Gati Lestari Endang Gati Lestari ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI Eny Widajati Erin Puspitarini Evriani Mareza, Evriani Fajar Sidiq, Abdul Rahman Faqih Udin Ghaling Achmad Giyanto Giyanto Giyanto, dan Gustiningsih, Dini HADIPOENTYANTI, ENDANG Hapshoh, Siti Hartati, RR. Sri Helfi Eka Saputra Herry Suhardiyanto Hesti Paramita Sari Hesti Paramita Sari Hidayat, dan Purnama Higuchi, Hirokazu HUSNI, ALI Ilham Dzikrillah Indra Gunawan Irma Isnafia Arief Irman Hermadi Irvansyah Irvansyah Iskandar Lubis Isna Tustiyani Iswari S. Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi JAJAH KOSWARA Jatsiyah, Venti Joko Ridho Witono Juharni Karlin Agustina Ketty Suketi Khaerin Nida Khairunnisa Lubis Kisman Kisman Kondo, Tomohiro KRISTIANTO NUGROHO, KRISTIANTO Kusmanto, . Laksono Trisnantoro Lestari, Puji Lestari, Sari Arie Lia Marliyanti Lia Marliyanti Linda Oktaviana Lisa Sentani Lubis, Khairunnisa Luky Adrianto Luluk Prihastuti Ekowahyuni M A Chozin MADE ASTAWAN Maharijaya, dan Awang MARIA BINTANG Marimin Marimin Marlina Mustafa, Marlina Maya Lestari, Maya Maya Melati Mercy Julinda Zebua Meuthia Rachmaniah Mohamad Rahmad Suhartanto Mu'arif, Mu'arif Muhamad Achmad Chozin Muhammad Hidayat Ginanjar, Muhammad Hidayat Muhammad Ridha Alfarabi Istiqlal Muhammad Ridha Alfarabi Istiqlal Muhammad Ridha Alfarabi Istiqlal Muhammad Rifqi Setio Aji Muhammad Syafi’i Muhammad Zairin Jr. Munarti Muthi'ah Khairun Nisa Muthi’ah Khairun Nisa Nabilah, Noorfakhriyah Ahsanti Nadila, Dea Nadiyah Mawaddah Ayuningtyas Nanda Chesaria Nangi, Moh. Guntur Nasution, Amal Ma’rup Nazirwan Nazirwan Neng Neni Neni, Neng Ninuk Purnaningsih Nugroho, Kristianto Nugroho, Rizki Anjal Puji Nugroho, Syarul Nur Wulandari Nura ,, Nura Nurbani, Hilmi Nurul Khumaida Odang Carman Okti Syah Isyani Permatasari Peni Lestari Permatasari, Okti Syah Isyani Perwira, Prajna Jaya Prayudani, Ayu Putri Gitanjali PUJI LESTARI Punjung Medaraji Suwarno PURNAMA HIDAYAT Putra, Bagas Akmala Putri, Astrid Aditya Putri, Faradila Danasworo Qadir, Abdul R Neny Iriany RAGAPADMI PURNAMANINGSIH Rahman Rahman Rahmawati, Rika Sri Rahmi Henda Yani RAHMI YUNIANTI Rahmi Yunianti RAHMI YUNIANTI Raisa Baharuddin Ratna Ningsih rawati ,, rawati Redy Gaswanto, Redy Reswari, Helvi Ardana Ria Ismiatun Ria Putri Ridwani, Sobir Riti, Estriana Ritonga, Arya W Ritonga, Arya Widura Ritonga, Arya Widura Rohim Firdaus ROHIM FIRDAUS ROHIM FIRDAUS Rokhana Faizah Rokhana Faizah Rosa Yunita Rosa Yunita Rosminah, Rosminah ROSSA YUNITA RR. Ella Evrita Hestiandari Rtionga, Arya Widura Rudy Hermanto Sadewi Maharani, Sadewi Sahid, Zulfikar Damaralam Sandra Arifin Aziz Sari Dewi, Okky Tiara Sautomo, Mohammad Wahyu Sayekti, Tri Wahono Dyah Ayu Sentani, Lisa Shandra Amarilis Shelvie Nidya Neyman Siahaan, Gretty Febriola Sianturi, Clarissa Yolanda Sintho Wahyuning Ardie Siti Marwiyah Siti Marwiyah Sobir Sobir Sobir Sobir Sobir Sobir, dan SRI HENDRASTUTI HIDAYAT Sri Nurdiati Sri Sujiprihati Sri Wahyuni Sri Wening Sri Wening, Sri Wening Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati SRIANI SUJIPRIHATI SRIANI SUJIPRIHATI Subandi Subandi Subekti, Isnani Suhaeb, Firdaus W. Suhartini, dan Tintin Suhesti, Sri Sukma, Dewi Sulassih, . Sundari, Fitria Suci Suprayanti Martia Dewi, Suprayanti Martia Surjono Hadi Sutjahjo Suwarto Suwarto Suwarto Syafi’i, Muhammad Syaidatul Rosidah Syaidatul Rosidah Syarifah Iis Aisyah Tatiek Kartika Suharsi Tatiek Kartika Suharsi Tefa, Anna Tefa, Anna Tengku Laila Kamaliah Theresia Dame Angel Lie Manurung Trias Sitaresmi Trikoesoemaningtyas Undang Undang Undang, Undang Verry Riyanto Vicente, Tobias Moniz Vitria Puspitasari Rahadi Wahid Rifan Gustoro Wahyu Widiyono Waras Nurcholis WATTIMENA, G. A. Wenny Amaliah Widodo , Widodo . Widodo Widodo Widodo Widodo Widodo, dan Willy Bayuardi Suwarno Winda Saskia Sijabat Winning Son Ashari Wulan Rahmawati Yesy Mardianawati Yohanes Hendrianto Yudilastari, Tiara Yudiwanti Wahyu E. Kusumo Yuli Rahmadi, Hernawan Yunandra Yundari, Yundari Yursida Yusnita Sari, Yusnita Yusuf Sumaryana Zebua, Mercy Julinda