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All Journal Media Kedokteran Hewan Prosiding Seminar Nasional Pakar Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Indian Journal of Forensic Medicine & Toxicology journal of Basic Medical Veterinary Narra J Buletin Pengabdian Journal of Fisheries & Marine Jurnal Agro Veteriner
Kadek Rachmawati
Division Of Basic Veterinary Faculty Of Veterinary Medicine Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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The Effectiveness Level of Gel Combination Binahong Leaf and Turmeric Rhizome Extract on The Mature Collagen of IIB Degree Burns in Rats Suryaningtyas, Ignasia Friska Amelia; Rachmawati, Kadek; Lukiswanto, Bambang Sektiari; Sudjarwo, Sri Agus; Soeharsono; Yuniarti, Wiwik Misaco
Media Kedokteran Hewan Vol. 34 No. 2 (2023): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v34i2.2023.102-115

Abstract

The aim of this research was to evaluate the effect of administering a combination gel of binahong leaf and turmeric rhizome extracts on mature collagen of IIB degree Burns in rat. A total of 25 male rats, 3 months of age, 150-200 gram body weight were used. This research consists of five treatments. P0 were normal skin, P1 were used 1% silver sulfadiazine, P2, P3, and P4 were gel combination of binahong leaf and turmeric rhizome extracts with increase concentration of binahong leaf extract i.e. 1.25%, 2.5%, and 5%, while turmeric rhizome extract i. e 2% for each treatment. Treatments have been given topically for 14 days, twice a day, started after burn wound application. At the end of the treatment period, skin excision was carried out, then the histopathological examination was performed. Microscopic observation on the wound healing process on mature collagen density showed that P0 was not a significant difference with P3 and P4, but P0 was significant difference with P1 and P2. The better burn healing process on P3 is allegedly because of the activity of saponin, and tannin, contained in the binahong leaf extract 2.5%, and curcumin in turmeric rhizome extract 2% have been proven to be effective for topical burns therapy.
The Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction Pinayungan, Probo Probo Warih Tatag; Aprinda Ratna Lovela; Eduardus Bimo Aksono; Ismudiono; Boedi Setiawan; Kadek Rachmawati; Jola Rahmahani
Media Kedokteran Hewan Vol. 35 No. 1 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i1.2024.21-27

Abstract

Sex identification of Sun Conures (Aratinga solstitialis) is crucial for breeding and preservation, as well as increasing sun conure populations. These birds are sexually monomorphic. Therefore, Determination between male and female carried out by their morphology examination. The Polymerase Chain Reaction (PCR) method, utilizing molecular-based technology, was employed to determine the sex of Aratinga solstitialis in this study. The P2 and P8 primers were utilized in this method, which has been deemed suitable and accurate for sex identification through calamus samples. The research focused on two 28-month-old Aratinga solstitialis birds. Calamus samples were collected and subjected to PCR amplification using the extracted calamus. The resulting PCR products were then visualized using electrophoresis with a 1% agarose gel. In the electrophoresis photo, the presence of two bands indicated a female specimen, whereas a single band indicated a male specimen. The result of the gel electrophoresis research showed that both of the Aratinga solstitialis were male with one band of each bird on ranged from 300-400 base pairs. The result show that the Polymerase Chain Reaction method in terms for sex identification on monomorphic birds, especially Aratinga solstitialis birds is very effective to differentiate the sex of young birds and the adults.
Effect of the Extract of Dayak Onions (Eleutherine palmifolia) on the Sperm Quality of Mice (Mus musculus) Induced with Monosodium Glutamate Rahmatullah, Aldin Akbar; Sugihartuti, Rahmi; Susilowati, Suherni; Hamid, Iwan Sahrial; Suprayogi, Tri Wahyu; Rachmawati, Kadek; Safitri, Erma; Mustofa, Imam; Mulyati, Sri
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.113-122

Abstract

This study aims to determine the effect of the extract of Dayak onions (Eleutherine palmifolia) on the motility, viability, and concentration of spermatozoa in mice (Mus musculus) induced with monosodium glutamate (MSG). This study involved 25 male mice aged 11 weeks and weighing approximately 20 g. The mice were divided into five groups, namely C- (CMC-Na 0.5%), C+ (4 mg/g BW of MSG), T1 (4 mg/g BW of MSG and 30 mg/kg BW of Dayak onion extract), T2 (4 mg/g BW of MSG and 60 mg/kg BW of Dayak onion extract), and T3 (4 mg/g BW of MSG and 120 mg/kg BW of Dayak onion extract). All treatments were administered for 52 days. The results showed no significant differences in the motility and concentration of spermatozoa between the C- (81.50 ± 4.18; 7.21 ± 0.37) and T3 (77 ± 2.74; 7.04 ± 0.13) groups (p < 0.05). In addition, the results showed significant differences in the viability of spermatozoa between the C- (83.30 ± 3.40) and T3 (77.20 ± 4.06) groups. Therefore, it can be concluded that the administration of Dayak onion extracts orally to mice induced with MSG can maintain the motility, viability, and concentration of spermatozoa.
Virgin coconut oil protected the diameter and the epithelium thickness of seminiferous tubules of mice (Mus musculus) from oral ethanol induction S Ravi, Vishantheni; Soeharsono, Soeharsono; Plumeriastuti, Hani; Rachmawati, Kadek
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.34-40

Abstract

This study investigated the preventive effect of virgin coconut oil (VCO) on the epithelium thickness and the diameter of the seminiferous tubules induced by ethanol in mice (Mus musculus). This study used 20 male mice as the experimental animal which were divided into five groups with four mice in each group. Negative control (C-) was given 2% Tween and aquadest, while positive control (C+) was given 2% Tween and 33% ethanol. T1, T2, and T3 were respectively given 0.09, 0.19, and 0.37 mL/kg bw VCO and 33% ethanol (0.2 mL/kg bw). VCO was given orally for 39 days, and ethanol was given orally seven days later for 32 days. Ethanol was administered two hours after the VCO administration. Analysis of variance followed by Tukey's range test on the epithelium thickness and the diameter of the seminiferous tubules showed significant differences (p <0.05) of C+ group from the other groups. Whereas, there was no significant difference (p >0.05) was found among C-, T1, T2 and T3 group. The result concluded that VCO could protect the testis of mice from the damage caused by ethanol.
Effect of taurine on histopathological features of spermatogenesis in seminiferous tubules of mice (Mus musculus) induced by paraquat Rahadi, Yustisiane Ruth; Tri Wahyu Suprayogi; Rahmi Sugihartuti; Kadek Rachmawati; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 2 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i2.2022.66-71

Abstract

This study aimed to determine the effect of taurine on the enhancement of the spermatogenetic process in male mice (Mus musculus) induced by paraquat (PQ). Twenty-five male mice (Mus musculus) aged 2-3 months with a bodyweight of around 35 grams were divided randomly into five groups. The K + and the treatment group (P1, P2, and P3) mice were induced using PQ. PQ was given intraperitoneally (IP) twice a week for 21 consecutive days at a dose of 30 mg/kg BW. Two hours after the administration of PQ, P1, P2, and P3 groups were given taurine at a dose of 250, 500, and 1000 mg/kg BW/day for three weeks (Heidari et al., 2019). K- group was given distilled water (IP) only. On day-29, mice were sacrificed for testicles histopathological preparations with hematoxylin-eosin staining. Results showed that the mice exposed to PQ only (the K+ group) had a reduced spermatogenesis score compared to those of the K- group (p <0.05).  Taurine treatment on PQ-exposed mice was followed by an increase spermatogenesis score. The optimal curative dose of taurine was 500 mg/kg (P2 group). However, a higher dose (1000 mg/kg BW) of taurine resulted in a decline in the spermatogenesis score than those of at the 500 mg/kg. It could be concluded that treatment with taurine could enhance the spermatogenetic process of male mice (Mus musculus) induced by PQ.
Peningkatan nilai produk olahan hasil laut dan gerakan gemar makan ikan bagi kelompok istri dan anak nelayan guna mencegah stunting [Increasing the value of processed sea products and the fish-eating movement for groups of fisherman wives and children to prevent stunting] Rachmawati, Kadek; Mulyati, Sri; Utama, Suzanita; Wurlina, W; Santoso, Kuncoro P.; Suwasanti, Ni Luh
Buletin Pengabdian Vol 4, No 1 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpengmas.v4i1.35366

Abstract

This Community Service Activity is carried out to educate fishermen's wives on how to process marine products such as fish into processed foods that children like so that children will be more fond of eating fish, processed innovations using raw materials from marine products into dry products and wet products with high-quality nutritional value with high selling value, and increasing the love of eating fish among fishing families to increase intelligence and prevent stunting. The methods used are education, training, and mentoring for innovations in processed seafood which are popular with children and have high selling value, apart from that, education is also carried out about stunting and how to prevent stunting. The result of this PKM activity is that fishermen's wives can make various foods and snacks made from marine products that are popular with children and have value for sale so that they can provide income for fishermen's wives, as well as increase the understanding of fishermen's wives about stunting to prevent the incidence of stunting in fishermen's children and increase the movement for eating fish among fishermen's families.
Comparative effects of epigallocatechin-3-gallate supplementation in skim milk, egg yolk-citrate, and combined extenders on the spermatozoa quality of Ettawah crossbred bucks during storage at 5 °C Muhammad Ikmal Robih; Anas Bad’rosana Prawindi; Tatik Hernawati; Budi Utomo; Suherni Susilowati; Tri wahyu Suprayogi; Sri Pantja Madyawati; Kadek Rachmawati; Mustofa Helmi Effendi; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 3 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i3.2025.147-158

Abstract

This study aimed to determine the quality of Ettawah crossbred buck semen diluted in skim milk, egg yolk-citrate extenders, or their combination, supplemented with epigallocatechin-3-gallate (EGCG). Semen was collected from Ettawah crossbred bucks using an artificial vagina. Six ejaculates were used for replication and divided into three extender treatments: T1, skim milk; T2, egg yolk-citrate; and T3, a combination of both, each supplemented with 1.5 µg/mL EGCG. The extended semen was stored in a refrigerator at 5 °C. Diluted samples were evaluated every 24 hours until spermatozoa motility declined to 30%, the minimum requirement for artificial insemination. The results showed that spermatozoa motility, viability, plasma membrane integrity, and morphological abnormality decreased during six days of storage at 5 °C. Spermatozoa motility remained above 30% for five days in T1 (36.50±1.22%) and T2 (41.67±2.06%), and for six days in T3 (43.33±1.03%). On day six, semen in the combined extender supplemented with EGCG (T3) showed significantly higher motility, viability, and membrane integrity, and lower morphological abnormalities (p <0.05) than the other treatments. In T2, all spermatozoa quality parameters were higher (p <0.05) than in T1. It can be concluded that the combined skim milk-egg yolk-citrate extender containing 1.5 µg/mL EGCG best maintained the spermatozoa quality of Ettawah crossbred bucks during six days of storage at 5 °C. Based on spermatozoa motility, the semen remained suitable for artificial insemination.
The effect of epigallocatechin 3-gallate (EGCG) on Sertoli and Leydig cells counts and spermatozoa quality of rats (Rattus norvegicus) exposed to monosodium glutamate Indira, Sitti Az-Zahra; A Ashiila Tsabita; susilowati, Suherni; Sri Pantja Madyawati; Yudaniayanti, Ira Sari; kusnoto, kusnoto; Legowo, Djoko; Hidajati, Nove; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 15 No. 1 (2026): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v15i1.2026.56-64

Abstract

This study aimed to determine the preventive effect of epigallocatechin 3-gallate (EGCG) on the number of Sertoli and Leydig cells in white rats (Rattus norvegicus) exposed to monosodium glutamate (MSG). This study used 25 male rats which were divided into five groups. The C– group was given only 1% Na-CMC, while the C+ group received 1% Na-CMC and 120 mg/kg BW of MSG. The T1, T2, and T3 groups were administered EGCG at doses of 4, 8, and 16 mg/kg BW, respectively, along with 120 mg/kg BW of MSG. All treatments were administered orally for 28 days. After the treatment period, the rats were sacrificed on day 29. Their testes were collected and processed into histological specimens using hematoxylin eosin (H&E) staining. The results showed that MSG exposure significantly decreased (p <0.05) the number of Sertoli and Leydig cells in the C+ group compared to the C– group. In contrast, the T1, T2, and T3 groups showed increased cell counts following EGCG administration. Epigalocathecin-3-gallate at a dose of 8 mg/kg BW showed the best potential in maintaining spermatozoa motility, viability, and plasma membrane integrity in rats exposed to MSG. EGCG, as an antioxidant, can reduce lipid peroxidation in the spermatozoa plasma membrane, thereby improving spermatozoa viability, motility, and plasma membrane integrity. In conclusion, the administration of EGCG mitigates the harmful effects of MSG on Sertoli and Leydig cell counts in white rats, with an optimal dose of 8 mg/kg BW.
Co-Authors . Kholik A Ashiila Tsabita Adiana Mutamsari Witaningrum Aditya Irawan Agustina, Firanda Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Amar Ma'ruf Fachrul Mu'minin Anas Bad’rosana Prawindi Anindya, Afifatus Annisa Nur Fitria Rahma Ansori, Arif NM. Anwar Ma'ruf Aprinda Ratna Lovela Ardhanella, Sellianova Atmaja, Peter Y. Bambang Sektiari Lukiswanto Billa, Lutfiah Annisa Bima, Dewangga Arya Boedi Setiawan BUDI UTOMO Bura, Maria Antonia Yersi Dua Chairul Anwar Dadik Rahardjo, Dadik Derkho, Marina Dewi, Fullan Ausati Putri Dini Fadhila Samjaya Durry, Fara D. Dwi Ananda, Novita Dwi Puspa Sukma Viranda Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Emy Koestanti Sabdoningrum Erma Safitri Ethica, Stalis N. Fajar Ramadhan Subiyantoro Febriano, Muhammad Galang Fedik Abdul Rantam Hamzah, Arshq Mirza Hani Plumeriastuti Hermin Ratnani Herry Agoes Hermadi Hidajanti, Nove Hidanah, Sri Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Humairah, Ira IMAM MUSTOFA Imam Mustofa Indira, Sitti Az-Zahra Indriyanti, Astrid Ira Sari Yudaniayanti Ismudiono Iva Nur Silviana Iwan Sahrial Hamid Iwan Sahrial Hamid Jamaluddin, Indra P. Khairullah, Aswin Rafif Kusnoto Kusnoto, Kusnoto Kusuma, Dhio Salhisya Marta Larasati, Anissa Dhea Legowo, Djoko Lilik Maslachah Lilik Maslachah Lita Rakhma Yustinasari Lucia Tri Suwanti, Lucia Tri Lukitasari, Lina Maharani, Disty Putri Meles, Dewa Ketut Mirino, Mario Navyseal Mirni Lamid Moch Juddy Widjadja Mohammad Sukmanadi Muhammad Farid Rizal Muhammad Hidayat Muhammad Ikmal Robih Murtadlo, Ahmad AA. Musa, Susan H. Mustofa Helmi Effendi Naw, Sin W. Nidom, Chairul Anwar Nove Hidajati Nunuk Dyah Retno Lastuti Nurul Azizah Nusdianto Triakoso Permatasari, Roselia Yuliani Pinayungan, Probo Probo Warih Tatag Prabowo, Gwenny Ichsan Pradana, Munawer Pudji Srianto Putri, Desak Ketut Sekar Cempaka Qurnianingsih, Ema Rahadi, Yustisiane Ruth Rahadian Zainul Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ramadhani, Fifi Fauziah Rara Irbah Fitriana Karimah Ratna Damayanti Ratna Damayanti Rebezov, Maksim Renaldi, Nogi Retno Sri Wahjuni Rimayanti Rimayanti Ristaqul Husna Belgania Riwu, Katty Hendriana Priscilia Rochmah Kurnijasanti Rusyda, Aqila sabila Rusyda, Tsamara Aulia S Ravi, Vishantheni Santoso, Kuncoro P. Santoso, Kuncoro Puguh Saraswati, Afif Tasya Sherina Lashita Candrakirana Siti Khaerunnisa Soeharsono Soeharsono Soeharsono Sri Agus Sudjarwo Sri Hidanah Sri Mulyati Sri Pantja Madyawati Suherni Susilowati Sukma Sahadewa, Sukma Sukmanadi, M Suryaningtyas, Ignasia Friska Amelia Suwarno Suwarno Suwarno Suwasanti, Ni Luh Suzanita Utama Tacharina, Martia Rani Tatik Hernawati Tri Wahyu Suprayogi Tyasningsih, Wiwiek Ummah, Fithriyah Cholifatul Ummah Utami, Prima Sukma Rizky Vensko Zico Dandi Wahyu Pratama Widiyatno, Thomas Valentinus Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wungu, Citrawati Dyah Kencono Wurlina, W Xin, Irene Teh Kai Yeni Dhamayanti Yosephi, Valensa Yulianna Puspitasari Zainal Arifin