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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Narra J Livestock and Animal Research Buletin Pengabdian Journal of Fisheries & Marine Jurnal Agro Veteriner
Kadek Rachmawati
Division Of Basic Veterinary Faculty Of Veterinary Medicine Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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The Effect of Giving Gel Combination of Binahong Leaf Extract and Turmeric Rhizome Extract on Histopathological Epithelial Thickness in II B Degree Burn of Rattus norvegicus Indriyanti, Astrid; Rachmawati, Kadek; Maslachah, Lilik; Damayanti, Ratna; Hidayatik, Nanik; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.45753

Abstract

This aim of this research was to study the effect of giving a combination gel of binahong (Anredera cordifolia) leaf and turmeric (Curcuma longa Linn) rhizome extract on histopathology of epithelial thickness which has II B degree burns. Twenty five male white rats (Rattus norvegicus) were divided into five groups. K(-) group was normal skin, K(+) group was skin burns treated with 1% silver sulfadiazine, P1, P2, and P3 was skin burns treated with gel combination of binahong leaf extract and turmeric rhizome extract with increase concentration of binahong leaf extract i.e. 1.25%, 2.5%, and 5%, while the concentration of turmeric rhizome extract i.e. 2% for each treatment. The combination gel of binahong leaf extrac 1.25% and turmeric rhizome extract 2% has potential to be an effective treatment for II B burns as indicated by increased of epithelial thickness. Therefore, this study concluded that 1.25% combination gel of binahong leaf extract and turmeric rhizome extract 2% is the effective dosage to increase epithelial thickness on II B healing degree burns wound healing.
Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro Saraswati, Afif Tasya; Sugihartuti, Rahmi; Puspitasari, Yulianna; Witaningrum, Adiana Mutamsari; Rachmawati, Kadek; Maslachah, Lilik; Raharjo, Hartanto Mulyo; Hisyam, Mirza Atikah Madarina; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51106

Abstract

This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.
The Effect of Red Ginger Extract (Zingiber officinale var rubrum) on The Thickness of The Epitelial and Diameter of Seminiferous Tubules in Albino Rat (Rattus norvegicus) Exposed Monosodium Glutamat Renaldi, Nogi; Rachmawati, Kadek; Widiyatno, Thomas Valentinus; Madyawati, Sri Pantja; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.51158

Abstract

Background: This study examined administration of MSG intraperitoneally to white rats resulted in a decrease in testicular weight, an increase in lipid peroxidase levels The testes, as the site of spermatogenesis, are highly vulnerable to oxidation processes caused by free  radicals,  which  can  disrupt spermatogenesis. Free radicals can be countered by antioxidants. One plant believed to have antioxidant activity is red ginger. Purpose: To determine the effect of red ginger against histopathology seminiferous tubules of white rat (Rattus norvegicus) exposed by monosodium glutamate. Methods: This study used 30 rats which were divided into 5 groups. The positve control group (K+) were given only monosodium glutamate 5g/kgBW and CMC Na orally, the treatments groups P1 were given monosodium glutamte 5g/KgBW + red ginger 50mg/rat/day, P2 were given 5g/KgBW + red ginger 100mg/rat/day, and P3 were given 5g/KgBW + red ginger 200mg/rat/day. All treatments were administered for 42 days. After 42 days of treatments, all rats were sacrificed and seminiferous tubules were observed with microscope from pathology laboratory. Results: Administration of red ginger extract can defend seminiferous tubules epithelial thicknes and diameter from exposure of monosodium glutamate with 100mg/KgBW dosage as the most effective dosage. Seminiferous tubules epitelial thickness and diameter were tested using one-way ANOVA and Duncan's multiple range test. Conclusion: Red Ginger Extract (Zingiber officinale var rubrum) has shown potential effects to counteract Monosodium Glutamat consequences on The Thickness of the epitelial and diameter of seminiferous tubules of white rat (Rattus norvegicus).
Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia Jamaluddin, Indra P.; Musa, Susan H.; Ethica, Stalis N.; Ansori, Arif NM.; Yosephi, Valensa; Atmaja, Peter Y.; Murtadlo, Ahmad AA.; Sahadewa, Sukma; Durry, Fara D.; Rebezov, Maksim; Derkho, Marina; Naw, Sin W.; Zainul, Rahadian; Rachmawati, Kadek
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.774

Abstract

Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gyrB gene. The BLAST program demonstrated that the sample had 99.89% similarity with P. aeruginosa strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gyrB gene exhibited 99.10% similarity with the P. aeruginosa strain PSA-1.2 (accession code KP172300.1).
Peningkatan pengetahuan untuk pencegahan stunting melalui sosialisasi dan demo pembuatan makanan balita berbahan dasar protein hewani bagi kelompok istri peternak sapi pedaging rumahan di Desa Lando, Lombok Timur, Nusa Tenggara Barat [Increasing knowledge for stunting prevention through socialization and demonstration for creating animal protein-based toddler food for a group of wives of home-based beef cattle farmers in Lando Village, East Lombok, West Nusa Tenggara] Qurnianingsih, Ema Ema; Rusyda, Aqila sabila; Hamzah, Arshq Mirza; Rachmawati, Kadek; Humairah, Ira Ira; Lukitasari, Lina; Khaerunnisa, St; Wungu, Citrawati Dyah Kencono; Ummah, Fithriyah Cholifatul Ummah; Kholik, Kholik; Riwu, Katty Hendriana Priscilia; Pradana, Munawer; Yansri, Alifianita Anake; Hidayat, Muhammad; Prabowo, Gwenny Ichsan
Buletin Pengabdian Vol 4, No 3 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v4i3.40293

Abstract

Stunting is a condition of failure to thrive in children under five (toddlers) which can hinder the child's physical and mental development. Data from the 2023 Indonesian Health Survey (SKI) shows that the number of stunted toddlers in West Nusa Tenggara (NTB) Province is still quite high, namely 24.6%. Stunting cannot be cured but can be prevented. One of the prevention efforts is to increase the knowledge of mothers or prospective mothers regarding stunting and preventing it by providing food with high nutrition. This Community Service Activity (PKM) was carried out with the aim of educating mothers and wives of home-based beef cattle farmers in Lando Village, East Lombok, NTB about preventing stunting by providing highly nutritious supplementary food (PMT) and stunting detection. The methods used were socialization and demonstration of making toddler food. The results of this PKM activity were that there was a significant increase in participants' knowledge regarding the material being socialized (p0.0001). The conclusion that can be drawn is that There had been an increase in knowledge among PKM participants regarding stunting and preventing stunting through providing food with high protein content.
Fish consumption and dementia prevention in the elderly: Evaluating the "Dudu Galak" program in the coastal community of Bulak, Surabaya [Konsumsi ikan dan pengurangan risiko demensia pada lansia: Implementasi program "Dudu Galak" pada masyarakat pesisir Bulak, Surabaya] Rachmawati, Kadek; Sukmanadi, M; Yudaniayanti, Ira Sari; Wurlina, W; Santoso, Kuncoro P.; Damayanti, Ratna; Putri, Desak Ketut Sekar Cempaka
Buletin Pengabdian Vol 5, No 1 (2025): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v5i1.43101

Abstract

In Indonesia, the elderly population is steadily increasing, and dementia is emerging as a significant public health concern. A lack of awareness regarding dementia prevention, particularly through nutrition-based interventions such as increased fish consumption, remains a major issue. This community service program aimed to educate fishermens wives and elderly residents by promoting the processing of fishery products, especially fish and shellfish, into various nutritious food products as a preventive measure against dementia. The program was implemented in Bulak, a coastal area of Surabaya, Indonesia, targeting fishermens wives and elderly community members through the Dudu Galak Program. The initiative focused on innovating the processing of local marine products into both dry and wet foods that are not only nutritionally rich but also economically valuable. The methods employed included educational outreach, hands-on training, and mentoring in the development of diverse, nutritious, and marketable seafood-based products. Additionally, the program provided education on dementia in older adults and strategies for its prevention. The results of this initiative demonstrated that fishermens wives and elderly residents successfully developed a variety of high-nutrition fish- and shellfish-based foods and snacks with promising market potential. Moreover, participants gained a better understanding of dementia and recognized that increasing fish consumption can serve as an effective preventive strategy. Through this program, it is expected that participants will improve their household income, enhance their knowledge of dementia, and promote greater fish consumption within fishing families as part of dementia prevention efforts among the elderly.
The Effects of Prebiotic Mannan Oligosaccharide (MOS) Supplementation on Hematology Profile of Native Chickens (Gallus gallus domesticus) Dewi, Fullan Ausati Putri; Utami, Prima Sukma Rizky; Rusyda, Tsamara Aulia; Kusuma, Dhio Salhisya Marta; Maharani, Disty Putri; Bima, Dewangga Arya; Rachmawati, Kadek
Journal of Basic Medical Veterinary Vol. 14 No. 1 (2025): Journal of Basic Medical Veterinary, June 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i1.66539

Abstract

Native chickens (Gallus gallus domesticus) are an important source of animal protein due to their adaptability and relatively simple management. However, their productivity remains limited, which poses a significant challenge in poultry farming. One promising strategy to improve the health and productivity of native chickens is supplementation with Mannan Oligosaccharides (MOS), a prebiotic known to enhance gut microflora balance, strengthen immune responses, and support overall health. This study aimed to evaluate the impact of MOS supplementation on the hematological profile of native chickens. A true experimental method with a control group pre-test post-test design was employed. The results demonstrated that MOS supplementation significantly improved the hematological profile of native chickens. Notable increases were observed in the red blood cell (RBC) count to 2.54 × 10⁶ cells/mm³, white blood cell (WBC) count to 20.30 × 10³ cells/mm³, hemoglobin concentration to 11.4 g/dL, and hematocrit value to 31%. These findings highlight the potential of MOS supplementation to enhance the health and productivity of Native chickens through improvements in key hematological parameters.
The effect of ketapang leaf extract (Terminalia catappa L.) on the number and type of leukocytes in broiler chickens Mirino, Mario Navyseal; Maslachah, Lilik; Widya Paramita Lokapirnasari; Rachmawati, Kadek; Tacharina, Martia Rani; Hidayatik, Nanik
Jurnal Agro Veteriner Vol. 8 No. 2 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i2.74193

Abstract

Broiler are popular meat-producing birds in Indonesia because they contain high levels of animal protein. Apart from being used in the treatment of infectious diseases, antibiotics are also used as antibiotic growth promoters (AGP). However, the use of antibiotics as feed additives has been prohibited, a natural substitute for AGP is katapang leaves (Terminalia catappa L.). This study aims to determine the effect of administering katapang leaf extract (Terminalia catappa L.) on the number and type of leukocytes in broiler. This study used 24 Cobb strain broiler which were divided into four treatment groups, each group consisting of six replications. The treatment groups were P0 = only given 1% CMC-Na solution, P1 = Katapang leaf extract solution with 5% concentration, P2 = Ketapang leaf extract solution with 10% concentration, P3 = Katapang leaf extract solution with 15% concentration. The treatment is given orally with a syringe, given to chickens aged 21–35 days. At the end of the study, 2 ml–3 ml of blood is taken from the brachial vein. Data were analyzed using Analysis of Variance and continued with Duncan's Multiple Range Test
Detection of Salmonella sp. in broiler chickens in closed houses using the polymerase chain reaction method Larasati, Anissa Dhea; Sabdoningrum, Emy Koestanti; Nidom, Chairul Anwar; Hidanah, Sri; Rachmawati, Kadek; Puspitasari, Yulianna
Jurnal Agro Veteriner Vol. 9 No. 1 (2025): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v9i1.78646

Abstract

This study aimed to detect the presence of Salmonella sp. in broiler chickens raised in closed house systems using the Polymerase Chain Reaction (PCR) method. Liver and intestinal samples were collected from chickens showing clinical signs such as diarrhea, anorexia, and lethargy, along with pathological lesions observed during necropsy. Bacteriological identification involved isolation using selective media, Gram staining and biochemical tests. Two samples (3A and 3B) showed colony morphology and biochemical characteristics consistent with Salmonella sp. Confirmation using PCR targeting the invA gene (primers 139 and 141, expected amplicon size 284 bp) yielded negative results in both samples, while the positive control successfully amplified the target. The inconsistency between bacteriological and molecular results may be attributed to several factors: absence or mutation of the invA gene in the tested isolates, primer mismatch with local Salmonella strains, or inadequate DNA quality and concentration. These findings suggest that reliance on a single molecular marker may be insufficient for accurate detection and underscore the importance of optimizing PCR conditions. The study highlights the need for locally adapted primers and complementary diagnostic approaches to improve the reliability of Salmonella sp. detection in poultry, particularly in intensive production systems like closed house environments.
The Implementation of Hazard Analysis and Critical Control Point (HACCP) in The Frozen Chicken Meat Distribution Chain in Sikka Regency Dua Bura, Maria Antonia Yersi; Effendi, Mustofa Helmi; Puspitasari, Yulianna; Rahardjo, Dadik; Tyasningsih, Wiwiek; Rachmawati, Kadek
VITEK : Bidang Kedokteran Hewan Vol. 14 No. 2 (2024): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v14i2.289

Abstract

Food safety has become a global issue. Hazard analysis and critical control points (HACCP) is a food safety assurance system in the world that emphasizes prevention efforts. This research is a qualitative descriptive epidemiological study which aims to examine the application of HACCP in the distribution chain of frozen chicken meat in Sikka Regency on the growth parameters of total bacteria, Salmonella, and antibiotic residues through field observations at supplier companies and retailers, as well as laboratory tests on 30 frozen chicken meat samples, and 30 fresh chicken meat samples as a comparison. Laboratory tests found antibiotic residues in 60% of samples (16.7% in fresh chicken meat), and microbial contamination in 33% of samples without Salmonella contamination (40% in fresh chicken meat without Salmonella contamination). Monitoring of antibiotic residues has not been carried out properly by supplier companies because the number of samples is very small, sales in markets without refrigeration facilities for up to five hours together with fresh chicken or near fresh fish allows contamination of chicken meat. HACCP principles have not been properly applied in the frozen chicken meat distribution chain. Guidance and regular monitoring are needed so that the quality of frozen chicken meat is maintained.
Co-Authors . Kholik Adiana Mutamsari Witaningrum Agustina, Firanda Aksono HP., Eduardus Bimo Anindya, Afifatus Ansori, Arif NM. Ardhanella, Sellianova Atmaja, Peter Y. Bambang Sektiari Lukiswanto Billa, Lutfiah Annisa Bima, Dewangga Arya Boedi Setiawan Bura, Maria Antonia Yersi Dua Chairul Anwar Dadik Rahardjo, Dadik Derkho, Marina Dewi, Fullan Ausati Putri Durry, Fara D. Dwi Ananda, Novita Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Ethica, Stalis N. Fedik Abdul Rantam Hamzah, Arshq Mirza Hani Plumeriastuti Hidajanti, Nove Hidanah, Sri Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Humairah, Ira Imam Mustofa IMAM MUSTOFA Indriyanti, Astrid Ira Sari Yudaniayanti Iva Nur Silviana Iwan Sahrial Hamid Jamaluddin, Indra P. Khairullah, Aswin Rafif Kusuma, Dhio Salhisya Marta Larasati, Anissa Dhea Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lukitasari, Lina Maharani, Disty Putri Meles, Dewa Ketut Mirino, Mario Navyseal Mirni Lamid Muhammad Hidayat Murtadlo, Ahmad AA. Musa, Susan H. Mustofa Helmi Effendi Naw, Sin W. Nidom, Chairul Anwar Nunuk Dyah Retno Lastuti Nurul Azizah Nusdianto Triakoso Permatasari, Roselia Yuliani Prabowo, Gwenny Ichsan Pradana, Munawer Putri, Desak Ketut Sekar Cempaka Qurnianingsih, Ema Rahadian Zainul Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Ratna Damayanti Rebezov, Maksim Renaldi, Nogi Ristaqul Husna Belgania Riwu, Katty Hendriana Priscilia Rochmah Kurnijasanti Rusyda, Aqila sabila Rusyda, Tsamara Aulia S Ravi, Vishantheni Sabdoningrum, Emy Koestanti Santoso, Kuncoro P. Santoso, Kuncoro Puguh Saraswati, Afif Tasya Siti Khaerunnisa Soeharsono Soeharsono Soeharsono Sri Mulyati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sukma Sahadewa, Sukma Sukmanadi, M Suryaningtyas, Ignasia Friska Amelia Suwarno Suwarno Suwasanti, Ni Luh Suzanita Utama Tacharina, Martia Rani Tri Wahyu Suprayogi Tyasningsih, Wiwiek Ummah, Fithriyah Cholifatul Ummah Utami, Prima Sukma Rizky Widiyatno, Thomas Valentinus Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wungu, Citrawati Dyah Kencono Wurlina, W Xin, Irene Teh Kai Yeni Dhamayanti Yosephi, Valensa Yulianna Puspitasari Zainal Arifin