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Screening of Blood Glucose Concentration in Domestic Cat (Felis Catus) Based on Body Condition Score, Breed, and Sex Using Portable Blood Glucose Meter Billa, Lutfiah Annisa; Triakoso, Nusdianto; Rachmawati, Kadek; Yuniarti, Wiwik Misaco; Aksono, Eduardus Bimo; Yudaniayanti, Ira Sari
Journal of Basic Medical Veterinary Vol. 12 No. 1 (2023): Journal of Basic Medical Veterinary, June 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i1.42246

Abstract

Type 2 diabetes mellitus (T2DM) is a common endocrine disease in domestic cats. Breed, sex, being overweight to an extent of obesity are predisposing factors for developing T2DM. One of the most common laboratory analyses conducted to confirm this disease is blood glucose concentrations. The aim of this research is to determine blood glucose concentration in domestic cats based on BCS, breed, and sex using a portable blood glucose meter. Research samples were obtained by taking blood samples from the marginal ear vein of 131 domestic cats that matched the sample's criteria, took at 2-hours post prandial, and drip onto the Sinocare strip. The results showed that the average blood glucose concentration was in the normal range, with a total of ideal (BCS ≥3/5) domestic cats (n=43) was 75.74 mg/dL, overweight (BCS ≥4/5) domestic cats (n=44) was 78.23 mg/dL, obese (BCS ≥5/5) domestic cats (n=44) was 89.66 mg/dL, male (n=51) was 80.57 mg/dL, female (n=80) was 81.69 mg/dL, Mixed Breed (n= 119) was 81.03 mg/dL, Angora (n=5) was 84.20 mg/dL, Persian (n=6) was 81.50 mg/dL and Ragdoll (n=1) was 92 mg/dL. Pearson correlation showed BCS (r=0.403; p= 0.000) had a moderate, positive, and significant correlation with blood glucose concentration in domestic cats. Meanwhile, the results of Kendall's Tau correlation showed that breed (p=0.740) and sex (p=0.555) had insignificant correlation with blood glucose concentration in domestic cats.
The Effectiveness of Ethanol Extract of Ketapang Leaves (Terminalia catappa L.) as a Larvicidal Against Aedes aegypti Mosquito Larvae Dwi Ananda, Novita; Rachmawati, Kadek; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Hidajanti, Nove; Meles, Dewa Ketut
Journal of Basic Medical Veterinary Vol. 12 No. 1 (2023): Journal of Basic Medical Veterinary, June 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i1.42248

Abstract

This study aimed to determine the effectiveness of the ethanol extract of ketapang leaves (Terminalia catappa) on mortality and histopathology of Ae. aegypti instar III as an effort to control Ae. aegypti as a disease vector without causing resistance to the larvae. The extraction process uses the maceration method. This type of research is a laboratory experiment with research subjects Ae. aegypti instar III of 700 individuals, which were divided into 7 treatment groups (1 negative control group using distilled water and 1% DMSO, 1 positive control group using 1000 ppm abate, ethanol extract of ketapang leaves with concentrations of 2000 ppm, 2500 ppm, 3500 ppm, 5000 ppm, 7000 ppm) and 4 repetitions. The results of this study indicate that the negative control (K-) mortality rate is 0% and the positive control (K+) mortality percentage is 100%. The results of the ethanol extract of ketapang leaves (Terminalia catappa) on the mortality of Ae. aegypti instar III in treatment one (P1) the percentage of mortality was 17%, treatment two (P2) the percentage of mortality was 21%, treatment three (P3) the percentage of mortality was 23%, treatment four (P4) the percentage of mortality was 27%, and treatment five (P5) the percentage of mortality is 42%. Histopathological observations on larvae that died after being exposed to ethanol extract of ketapang leaves (Terminalia catappa) for 24 hours damaged the basement membrane, peritropic membrane and epithelial cells. It can be concluded that the ethanol extract of ketapang leaves (Terminalia catappa) is effective as a larvicidal against Ae. aegypti instar III but can cause damage to the midgut and cause death of the larvae.
The Effect of Giving Gel Combination of Binahong Leaf Extract and Turmeric Rhizome Extract on Histopathological Epithelial Thickness in II B Degree Burn of Rattus norvegicus Indriyanti, Astrid; Rachmawati, Kadek; Maslachah, Lilik; Damayanti, Ratna; Hidayatik, Nanik; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.45753

Abstract

This aim of this research was to study the effect of giving a combination gel of binahong (Anredera cordifolia) leaf and turmeric (Curcuma longa Linn) rhizome extract on histopathology of epithelial thickness which has II B degree burns. Twenty five male white rats (Rattus norvegicus) were divided into five groups. K(-) group was normal skin, K(+) group was skin burns treated with 1% silver sulfadiazine, P1, P2, and P3 was skin burns treated with gel combination of binahong leaf extract and turmeric rhizome extract with increase concentration of binahong leaf extract i.e. 1.25%, 2.5%, and 5%, while the concentration of turmeric rhizome extract i.e. 2% for each treatment. The combination gel of binahong leaf extrac 1.25% and turmeric rhizome extract 2% has potential to be an effective treatment for II B burns as indicated by increased of epithelial thickness. Therefore, this study concluded that 1.25% combination gel of binahong leaf extract and turmeric rhizome extract 2% is the effective dosage to increase epithelial thickness on II B healing degree burns wound healing.
Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro Saraswati, Afif Tasya; Sugihartuti, Rahmi; Puspitasari, Yulianna; Witaningrum, Adiana Mutamsari; Rachmawati, Kadek; Maslachah, Lilik; Raharjo, Hartanto Mulyo; Hisyam, Mirza Atikah Madarina; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51106

Abstract

This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.
The Effect of Red Ginger Extract (Zingiber officinale var rubrum) on The Thickness of The Epitelial and Diameter of Seminiferous Tubules in Albino Rat (Rattus norvegicus) Exposed Monosodium Glutamat Renaldi, Nogi; Rachmawati, Kadek; Widiyatno, Thomas Valentinus; Madyawati, Sri Pantja; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.51158

Abstract

Background: This study examined administration of MSG intraperitoneally to white rats resulted in a decrease in testicular weight, an increase in lipid peroxidase levels The testes, as the site of spermatogenesis, are highly vulnerable to oxidation processes caused by free  radicals,  which  can  disrupt spermatogenesis. Free radicals can be countered by antioxidants. One plant believed to have antioxidant activity is red ginger. Purpose: To determine the effect of red ginger against histopathology seminiferous tubules of white rat (Rattus norvegicus) exposed by monosodium glutamate. Methods: This study used 30 rats which were divided into 5 groups. The positve control group (K+) were given only monosodium glutamate 5g/kgBW and CMC Na orally, the treatments groups P1 were given monosodium glutamte 5g/KgBW + red ginger 50mg/rat/day, P2 were given 5g/KgBW + red ginger 100mg/rat/day, and P3 were given 5g/KgBW + red ginger 200mg/rat/day. All treatments were administered for 42 days. After 42 days of treatments, all rats were sacrificed and seminiferous tubules were observed with microscope from pathology laboratory. Results: Administration of red ginger extract can defend seminiferous tubules epithelial thicknes and diameter from exposure of monosodium glutamate with 100mg/KgBW dosage as the most effective dosage. Seminiferous tubules epitelial thickness and diameter were tested using one-way ANOVA and Duncan's multiple range test. Conclusion: Red Ginger Extract (Zingiber officinale var rubrum) has shown potential effects to counteract Monosodium Glutamat consequences on The Thickness of the epitelial and diameter of seminiferous tubules of white rat (Rattus norvegicus).
Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia Jamaluddin, Indra P.; Musa, Susan H.; Ethica, Stalis N.; Ansori, Arif NM.; Yosephi, Valensa; Atmaja, Peter Y.; Murtadlo, Ahmad AA.; Sahadewa, Sukma; Durry, Fara D.; Rebezov, Maksim; Derkho, Marina; Naw, Sin W.; Zainul, Rahadian; Rachmawati, Kadek
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.774

Abstract

Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identified with molecular identification. Sequencing and BLAST analysis were carried out to determine the similarity of gene identity by comparing sample sequence with other isolate sequences on the Gene Bank. The results of molecular identification showed amplification DNA band of around 934 base pairs (bp) for 16S rRNA and 225 bp for gyrB gene. The BLAST program demonstrated that the sample had 99.89% similarity with P. aeruginosa strain XC4 (accession code ON795960.1) for the 16S rRNA gene. Meanwhile, the gyrB gene exhibited 99.10% similarity with the P. aeruginosa strain PSA-1.2 (accession code KP172300.1).
Porang (Amorphophallus oncophyllus) tuber extract improved the histopathological features of diabetic rat (Rattus norvegicus) testicles Dwi Puspa Sukma Viranda; Rochmah Kurnijasanti; Kadek Rachmawati; Iwan Sahrial Hamid; Pudji Srianto; Hani Plumeriastuti; Lita Rakhma Yustinasari
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.47-57

Abstract

This study aims to determine the effect of porang (Amorphophallus oncophyllus) tuber extract on the histopathological features of the testicle of alloxan induced rats (Rattus norvegicus). Thirty male rats were randomly divided into six groups. Rats in group C- were injected with distilled water, while rats in groups C+, T0, T1, T2, and T3 were injected with 120 mg/kg bw alloxan. Blood glucose was measured three days after alloxan injection. Rats in groups C- and C+ were then administered with 1% Na-CMC, whereas rats in groups T0, T1, T2, and T3 were then administered with 45mg/kg bw metformin, and 100, 200, and 400 mg/kg bw of porang tuber extract, respectively. All solution were given orally once every day for 14 days. Spermatogenic activity was assessed using the Johnsen scoring system and analyzed by Kruskal-Wallis, followed by the Mann-Whitney test. The diameter and epithelial thickness of the seminiferous tubule were measured using image raster software and analyzed using Anova followed by Duncan's test. The results showed that spermatogenesis score, diameter, and epithelial thickness of seminiferous tubule of group C+ were smaller (p <0.05) than group C-. Spermatogenesis scores of groups T0, T1, T2, and T3 were higher (p <0.05) than group C+. Seminiferous tubule diameter and epithelial thickness in groups T0, T1, and T2 were greater (p <0.05) than group C+. It could be concluded that porang tuber extract at a dose of 200 mg/kg bw improved the spermatogenesis score, diameter seminiferous tubule, and thickness of the epithelium of diabetic rats.
The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

Abstract

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.
Mulberry (Morus alba L.) leaf extract enhanced spermatozoa motility, viability, and plasma membrane integrity of rats (Rattus norvegicus) exposed to e-cigarette smoke Ramadhani, Fifi Fauziah; Yulianna Puspitasari; Hermin Ratnani; Budi Utomo; Rochmah Kurnijasanti; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.120-128

Abstract

This study investigates the impact of mulberry leaf extract on the viability, plasma membrane integrity, and motility of spermatozoa from male white rats (Rattus norvegicus) exposed to e-cigarette smoke. A total of twenty-five male rats were divided into five groups: negative control (NC), positive control (PC), T1, T2, and T3. All groups, except for the NC group, were exposed to e-cigarette smoke. Rats in the T1, T2, and T3 groups received mulberry leaf extract in doses of 100, 200, and 400 mg/kg bw, respectively, while the NC and PC groups were given a placebo of 1% Na-CMC. Both the mulberry leaf extract and the placebo were administered daily, beginning three days prior to the start of e-cigarette smoke exposure, which lasted for 28 days. Results showed that spermatozoa motility, plasma membrane integrity, and viability in the experimental groups were significantly lower than those in the NC group (p <0.05). Conversely, rats in the T1, T2, and T3 groups that received mulberry leaf extract demonstrated significantly greater spermatozoa viability, plasma membrane integrity, and motility compared to the PC group (p <0.05). The T3 group exhibited the most pronounced improvements, with significantly enhanced spermatozoa viability, membrane integrity, and motility (p <0.05) relative to the PC group. These results indicate that mulberry (Morus alba L.) leaf extract enhanced spermatozoa viability, plasma membrane integrity, and motility in white rats (Rattus norvegicus) subjected to e-cigarette smoke.
Peningkatan pengetahuan untuk pencegahan stunting melalui sosialisasi dan demo pembuatan makanan balita berbahan dasar protein hewani bagi kelompok istri peternak sapi pedaging rumahan di Desa Lando, Lombok Timur, Nusa Tenggara Barat [Increasing knowledge for stunting prevention through socialization and demonstration for creating animal protein-based toddler food for a group of wives of home-based beef cattle farmers in Lando Village, East Lombok, West Nusa Tenggara] Qurnianingsih, Ema Ema; Rusyda, Aqila sabila; Hamzah, Arshq Mirza; Rachmawati, Kadek; Humairah, Ira Ira; Lukitasari, Lina; Khaerunnisa, St; Wungu, Citrawati Dyah Kencono; Ummah, Fithriyah Cholifatul Ummah; Kholik, Kholik; Riwu, Katty Hendriana Priscilia; Pradana, Munawer; Yansri, Alifianita Anake; Hidayat, Muhammad; Prabowo, Gwenny Ichsan
Buletin Pengabdian Vol 4, No 3 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v4i3.40293

Abstract

Stunting is a condition of failure to thrive in children under five (toddlers) which can hinder the child's physical and mental development. Data from the 2023 Indonesian Health Survey (SKI) shows that the number of stunted toddlers in West Nusa Tenggara (NTB) Province is still quite high, namely 24.6%. Stunting cannot be cured but can be prevented. One of the prevention efforts is to increase the knowledge of mothers or prospective mothers regarding stunting and preventing it by providing food with high nutrition. This Community Service Activity (PKM) was carried out with the aim of educating mothers and wives of home-based beef cattle farmers in Lando Village, East Lombok, NTB about preventing stunting by providing highly nutritious supplementary food (PMT) and stunting detection. The methods used were socialization and demonstration of making toddler food. The results of this PKM activity were that there was a significant increase in participants' knowledge regarding the material being socialized (p0.0001). The conclusion that can be drawn is that There had been an increase in knowledge among PKM participants regarding stunting and preventing stunting through providing food with high protein content.
Co-Authors . Kholik A Ashiila Tsabita Adiana Mutamsari Witaningrum Aditya Irawan Agustina, Firanda Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Amar Ma'ruf Fachrul Mu'minin Anas Bad’rosana Prawindi Anindya, Afifatus Annisa Nur Fitria Rahma Ansori, Arif NM. Anwar Ma'ruf Aprinda Ratna Lovela Ardhanella, Sellianova Atmaja, Peter Y. Bambang Sektiari Lukiswanto Billa, Lutfiah Annisa Bima, Dewangga Arya Boedi Setiawan BUDI UTOMO Bura, Maria Antonia Yersi Dua Chairul Anwar Dadik Rahardjo, Dadik Derkho, Marina Dewi, Fullan Ausati Putri Dini Fadhila Samjaya Durry, Fara D. Dwi Ananda, Novita Dwi Puspa Sukma Viranda Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Emy Koestanti Sabdoningrum Erma Safitri Ethica, Stalis N. Fajar Ramadhan Subiyantoro Febriano, Muhammad Galang Fedik Abdul Rantam Hamzah, Arshq Mirza Hani Plumeriastuti Hermin Ratnani Herry Agoes Hermadi Hidajanti, Nove Hidanah, Sri Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Humairah, Ira Imam Mustofa IMAM MUSTOFA Indira, Sitti Az-Zahra Indriyanti, Astrid Ira Sari Yudaniayanti Ismudiono Iva Nur Silviana Iwan Sahrial Hamid Iwan Sahrial Hamid Jamaluddin, Indra P. Khairullah, Aswin Rafif Kusnoto Kusnoto, Kusnoto Kusuma, Dhio Salhisya Marta Larasati, Anissa Dhea Legowo, Djoko Lilik Maslachah Lilik Maslachah Lita Rakhma Yustinasari Lucia Tri Suwanti, Lucia Tri Lukitasari, Lina Maharani, Disty Putri Meles, Dewa Ketut Mirino, Mario Navyseal Mirni Lamid Moch Juddy Widjadja Mohammad Sukmanadi Muhammad Farid Rizal Muhammad Hidayat Muhammad Ikmal Robih Murtadlo, Ahmad AA. Musa, Susan H. Mustofa Helmi Effendi Naw, Sin W. Nidom, Chairul Anwar Nove Hidajati Nunuk Dyah Retno Lastuti Nurul Azizah Nusdianto Triakoso Permatasari, Roselia Yuliani Pinayungan, Probo Probo Warih Tatag Prabowo, Gwenny Ichsan Pradana, Munawer Pudji Srianto Putri, Desak Ketut Sekar Cempaka Qurnianingsih, Ema Rahadi, Yustisiane Ruth Rahadian Zainul Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmi Sugihartuti Rahmi Sugihartuti, Rahmi Ramadhani, Fifi Fauziah Rara Irbah Fitriana Karimah Ratna Damayanti Ratna Damayanti Rebezov, Maksim Renaldi, Nogi Retno Sri Wahjuni Rimayanti Rimayanti Ristaqul Husna Belgania Riwu, Katty Hendriana Priscilia Rochmah Kurnijasanti Rusyda, Aqila sabila Rusyda, Tsamara Aulia S Ravi, Vishantheni Santoso, Kuncoro P. Santoso, Kuncoro Puguh Saraswati, Afif Tasya Sherina Lashita Candrakirana Siti Khaerunnisa Soeharsono Soeharsono Soeharsono Sri Agus Sudjarwo Sri Hidanah Sri Mulyati Sri Pantja Madyawati Suherni Susilowati Sukma Sahadewa, Sukma Sukmanadi, M Suryaningtyas, Ignasia Friska Amelia Suwarno Suwarno Suwarno Suwasanti, Ni Luh Suzanita Utama Tacharina, Martia Rani Tatik Hernawati Tri Wahyu Suprayogi Tyasningsih, Wiwiek Ummah, Fithriyah Cholifatul Ummah Utami, Prima Sukma Rizky Vensko Zico Dandi Wahyu Pratama Widiyatno, Thomas Valentinus Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wungu, Citrawati Dyah Kencono Wurlina, W Xin, Irene Teh Kai Yeni Dhamayanti Yosephi, Valensa Yulianna Puspitasari Zainal Arifin