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Journal : journal of Basic Medical Veterinary

IDENTIFIKASI BAKTERI Escherichia coli PENGHASIL Extended Spectrum β-Lactamase DARI SWAB RECTAL SAPI PERAH MENGGUNAKAN METODE VITEK-2 DI KUD TANI WILIS SENDANG KABUPATEN TULUNGAGUNG Syah Putra, Akyun Rozaqi; Effendi, Mustofa Helmi; Koesdarto, Setiawan; Suwarno, Suwarno; Tyasningsih, Wiwik; Soelih Estoepangestie, Agnes Theresia
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20414

Abstract

Antibiotic resistance in animals and humans has become a global problem that needs attention. The use of antibiotics in inappropriate on food-producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). The aim of this study was to isolate and identified ESBL- E. coli isolate from dairy cow rectal swabs in Sendang, Tulungagung district using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The result of the study showed that from all of the samples could be isolated and indentified E. coli, based on the colony characteristics on EMBA and biochemical test. Based on the double disc synergy test method using antibiotic disc amoxicylyn-clavulanate, ceftriaxone, aztreonam, ceftazidime and cefotaxime, 10 isolates could be identify els ESBL- E. coli. furthermore 3 out of 10 isolates DDST positives were confirmed ESBL- E. coli using Vitek-2 method.
Duodenum Histomorphology of Bacillus subtilis and Bacillus coagulan Isolate in Laying Hen Strain Isa Brown Muzhaffar, Adam Maulana; Hamid, Iwan Sahrial; Effendi, Mustofa Helmi; Wibawati, Prima Ayu; Sukmanadi, Mohammad; Solfaine, Rondius
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.48030

Abstract

Probiotics are live microbes that are used as supplementary feed that work to increase the good bacteria that live in the digestive tract by suppressing pathogenic microorganisms in the intestine so that good bacteria can grow can optimize the feed absorption process. This study aims to determine the effect of adding probiotics as feed additive Against the height and width of the duodenal villi of the laying chicken in early production. Probiotics can nourish the digestive tract and increase nutrient digestibility so that nutrient intake is met for livestock. Bacillus subtilis and Bacillus coagulans as a prophylaxis against bacterial diseases by balancing intestinal microbes, increasing digestibility, and secreting protease, lipase, and amylase enzymes. The purpose of this study was to determine the effect of probiotics Bacillus subtilis and Bacillus coagulans on duodenal histomorphology. Twenty-four layers were randomly divided into 4 groups. The control group was not given probiotics, groups P1, P2, and P3 were given probiotics of 2 ml, 4 ml, and 6 ml. Probiotics were given for 14 days. One-way ANOVA showed a significant difference in each group (P<0.05) and continued with the DUNCAN test. The average length and width of the duodenal villi in each treatment showed that the control group was significantly different from the P1, P2, and P3 groups. The P3 group had the highest average length of duodenal villi. The increase in villi height and villi width is due to the increase in short-chain fatty acids induced by probiotics. An increase in the height and width of villi indicates the absorption capacity of the small intestine, the work of digestive enzymes on the intestinal mucosa is more maximal in the intestine with a wider morphological size, so that absorption and growth are better. The conclusion from the research that has been done is that the use of Bacillus subtilis and Bacillus coagulans as probiotics has an effect on the layer duodenal villi which is characterized by an increase in the average length of the duodenal villi according to the increase in the probiotic dose.
Co-Authors A. T. Soelih Estoepangestie Adiana Mutamsari Witaningrum Adrenalin, Sruti Listra Al Arif, M Anam Amri, Indah Amalia Anam Al Arif Anas Bad’rosana Prawindi Annisa, Suwaibatul Annise Proboningrat Arifah, Sitti Asri Rizky Asri Rizky Astuti, Elita Sriwidya Bambang Sektiari Lukiswanto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Benjamin Christoffel Tehupuring, Benjamin Christoffel Boedi Setiawan BUDI UTOMO Budiarto Budiarto Bura, Maria Antonia Yersi Dua Chusniati, Sri Dadik Rahardjo, Dadik Dadik Raharjo Dameanti, Fidi Nur Aini Eka Puji Dewi, Aprilia Kurnia Dian Ayu Permatasari Dian Ayu Permatasari Didik Handijatno Didik Handijatno Didik Handijatno Elisa Herina Dimariwu Endah, Tri Endang Suprihati Eriza Rosalina Rochmah Estoepangestie, Agnes Theresia Soelih Fadlilah, Shinta Levea Ni'matul Fatmawati, Mira Fatmawati Fauziah, Ima Fedik Abdul Rantam Habibi, Muhammad Afif Hani Plumeriastuti Harijani, Nenny Hasib, Abdullah Hernanda, Ary Setya Herry Agoes Hermadi Istiana, Izzatul Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Khoiru Indana Kinasih, Kurnia Nisa Kurniawan, Muhammad ‘Ahdi Kusala, Muhammad Khaliim Jati Kusriningrum Rochiman Lailatul Maghfiroh Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luthfiana, Nurul Masdiana C Padaga Maudika Pamela Fiorenza Mirni Lamid Mohammad Sukmanadi Moses, Ikechukwu Benjamin Mufasirin Muhammad Ikmal Robih Mustika, Yolla Rona Muzhaffar, Adam Maulana Nenny Harijani Nunuk Dyah Retno Lastuti Nusdianto Triakoso Permatasari, Dian Ayu Pradika, Adhitya Yhoga Purbowati, Tri Endah Putri, Mariana Febrilianti Resilinda Rahaju Ernawati Raharjo, Dadik Rahmahani, Jola Rahmandari, Dina Agylia Raissa, Ricadonna Ramandinianto, Sancaka Chasyer Residiwati, Gretania Rochmah Kurnijasanti Sabdoningrum, Emy Koestanti Safitri, Devi Ayu Saputra, Rakan Mahiid Sari, Fifin Kurnia Sarudji, Suryanie Setiawan Koesdarto Sevitasari, Adelita Putri Sheila Marty Yanestria Sitti Arifah Soeharsono Soeharsono Soelih Estoepangestie, Agnes Theresia Sri Hidanah Sri Mulyati Sri Mumpuni Sosiawati Sri Pantja Madyawati Subagyo, Safira Fauziyyah Suherni Susilowati Sukmanadi, Mohammad Supriyadi Suwarno Suwarno Syah Putra, Akyun Rozaqi Syah, Ali Akram Tacharina, Martia Rani Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati Tri Untari Tri Untari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Tyasningsih, Wiwiek Tyasningsih, Wiwik Veena Vasheene Vijaya Kumar Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Wibawati, Prima Ayu Wibisono, Freshinta Jellia Widi Nugroho Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Zahro, Vida Seanita