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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Fitopatologi Indonesia Vegetalika Jurnal Sain Veteriner Jurnal Ilmu Pertanian Jurnal Penelitian Saintek Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Jurnal Natur Indonesia Jurnal Pendidikan Matematika dan IPA Prosiding Seminar Biologi Bioedukasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Jurnal Sains dan Teknologi BUNGAMPUTI Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Hutan Tanaman Jurnal Riset Ekonomi & Bisnis Biogenesis: Jurnal Ilmiah Biologi Teknosains: Media Informasi Sains dan Teknologi Biotropika Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Program Kreativitas Mahasiswa - Penelitian Indonesian Journal of Biotechnology Biota Biology, Medicine, & Natural Product Chemistry Planta Tropika Bioeksperimen: Jurnal Penelitian Biologi Majalah Obat Tradisional Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology INDONESIAN JOURNAL OF PHARMACY Scientiae Educatia: Jurnal Pendidikan Sains BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Ilmiah KOMPUTASI Jurnal Biodjati PROCEEDING ICBS Biosaintifika: Journal of Biology & Biology Education Paradigma POLISTAAT: Jurnal Ilmu Sosial dan Ilmu Politik Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) jurnal Ilmu Pertanian (Agricultural Science) INSIST (International Series on Interdisciplinary Research) Jurnal Teknologi Pertanian Andalas Jurnal Riset Pendidikan Kimia (JRPK) Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Jurnal JKFT Jurnal Pembelajaran dan Biologi Nukleus Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Akuntansi Food ScienTech Journal Sains dan Matematika International Journal of Environment, Engineering, and Education Jurnal Agrotropika Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences International Journal of Environment, Engineering & Education Pasundan International of Community Services Journal (PICS-J) ABDI MOESTOPO: Jurnal Pengabdian pada Masyarakat Proceeding Biology Education Conference Berkala Penelitian Hayati Journal of Innovation in Community Empowerment (JICE) Comsep : Jurnal Pengabdian Kepada Masyarakat Jurnal Jarlit Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Filogeni: Jurnal Mahasiswa Biologi Jurnal Kedokteran Hewan Berkala Ilmiah Biologi Journal of Fisheries & Marine BIO-SAINS | Jurnal Ilmiah Biologi Indonesian Journal of Jamu BIOTROPIC
BUDI SETIADI DARYONO
Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
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Journal : Jurnal Veteriner

 1 
Identifikasi Escherichia coli O157:H7 serta Deteksi Gen Shiga Like Toxin 1 dan 2 Asal Feses Hewan, Daging, dan Feses Manusia I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Daryono
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Escherichia coli O157:H7 with the ability to produce shiga-like toxin was isolated from beef, cattle,chicken, and human feces. Due to its importance to human health, it is necessary to identify the genesencoding the production of shiga-like toxin, stx1 and stx2 respectively to further understand the pathogenesis.Isolation of E. coli was done on Eosin Methylene Blue Agar (EMBA), followed by identification on SorbitolMacConkey Agar (SMAC), latex agglutination test, and H7 antiserum test, respectivelly. The existence ofgenes stx1 and stx2 in E. coli O157:H7 was confirmed molecularly using PCR method with specific primersLP 30/31 and LP 43/44, Stx2 (F)/Stx2 (R) respectively. Escherichia coli O157:H7 was isolated from 22 outof 344 samples (6,4%). Some isolates showed gene stx1 and stx2 was detected in two isolates as indicatedby a 384 bp band (stx1 gene), 584 bp and 1588 bp bands (stx2 gene) respectivelly. The results indicatedthat local isolates E. coli O157:H7 are potential as a zoonoses agent.
Pengembangan Antibodi Poliklonal dari Stadium Oosista, Sporosista, dan Sporozoit Eimeria tenella (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) Galuh Tresnani; Joko Prastowo; Wisnu Nurcahyo; Budi Setiadi Daryono
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The research on developing diagnostic method, vaccine, and drugs for coccidiosis has been focused onthe finding of the immunogenic molecule in Eimeria. The identification of this agent will need the antibodywhich can recognize the biomolecule in the antigen. Antibody that has been developed for this purposeshould be analyzed first, and one of the simple methods for analyzing this antibody is through dot blotanalysis. The objective of this research was to analyze the polyclonal antibody which developed from theoocyst, sporocyst, and sporozoite of  E. tenella using dot blot analysis. The antigen for this polyclonalantibody was made from each of the E. tenella stadium by sonication. Fifteen mice, divided into 3 groups,were then injected subcutaneously with each antigen. The sera from these mice were then collected, analyzedby using ELISA and then it will be used for the dot blot analysis. The research result showed that thepolyclonal antibody which has been developed in mice from each antigen can react with the antigen itself.From this result it can be concluded that the developing of this antibody is successful and it can be used forfurther research in immunoproteomic.
Studi Epidemiologi Agen Zoonosis Escherichia coli O157:H7 melalui Analisis Random Amplification of Polymorphic DNA (RAPD) I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Daryono
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Epidemiological studies of zoonotic agent Escherichia coli O157:H7 have been analyzed pheneticallyand or phylogenetically. In a phenetic classification, micoorganisms are arranged into groups (phena) onthe basis of high overall similarity using both phenotypic and genotypic methods without judgementaspect of its ancestry or evolutionary. Due to its importance to epidemiological aspect, the study of geneticvariation of isolates origin from some sources need to be conducted in order to trace the routes of infection.A total of 20 samples obtained from some sources i.e clinically human feces, non-clinically human feces,cattle feces, chicken feces, and beef feces were used in this study. The study was started by confirming allof the isolates using O157 latex agglutination test and H7 antiserum test, followed by genomic DNAanalysis by random amplification of polymorphic DNA /RAPD methods. RAPD results were analyzed using a simple matching coeficient (Ssm) and alogorhythm unweighted pair group method using arithmeticaverages (UPGMA) programe. Results showed there were range of genetic DNA from local isolates (75.1–99,6%) which was almost similar to ATCC 43894 control isolate. The highest similarity (99.6%) to ATCC43894 control was showed by SM-7(1) isolate obtained from cattle fecal and KL-68(1), isolate obtainedfrom clinically human fecal. In addition, KL-52(7) obtained from clinically human fecal had high similarity(99.6%) to MK-35 isolate obtained from chicken fecal. On the other hand, DS-21(4) and DS-16(2) isolatesthat were obtained from beef had high similarity (84.9%) to other isolates including ATCC 43894 controlisolate. The highest similarity of E. coli O157:H7 isolates that were obtained from cattle feces, beef, andchicken feces to human feces isolate indicated that there were both cattle and chicken were potentialreservoirs of the zoonotic agen which can be transmitted to human.
Profil Protein Stadium Sporozoit Eimeria tenella Isolat Yogyakarta Melalui Analisis Protein SDS-PAGE (PROTEIN PROFILE OF THE SPOROZOITE OF Eimeria tenella ISOLATES FROM YOGYAKARTA USING SDS-PAGE PROTEIN ANALYSIS) Galuh Tresnani; Joko Prastowo; Wisnu Nurcahyo; Budi Setiadi Daryono
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of thedisease is between 80 to 90%. A rapid and prompt diagnosis would be one of the essential steps ineradication and control of the disease. The objective of this study is to determine the protein profile ofsporozoite of Eimeria tenella isolated in Yogyakarta using sodium dodecyl sulphate polyacrilamide gelelectrophoresis (SDS-PAGE) protein analysis. Protein analysis was performed in 12% polyacrilamide geland further electrophoresis at 100 volts and over-staining with Coomasie brilliant blue. The resultsshowed that the sporozoite of E. tenella isolated in Yogyakarta contained five proteins with molecularweights of 15, 26, 32, 80, and 91 kDa, respectively.
Pewarisan Karakter Fenotip Ayam Hasil Persilangan Ayam Pelung dengan Ayam Cemani Budi Setiadi Daryono; Iwan Roosdianto; Hendry Tri Sakti Saragih
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The germ plasm variability of Indonesian local chicken is well known and one species which known as“ayam Pelung” originated from West Java is famous for its meat. The present study aimed to observed thephenotype characters i.e. fur color and body weight in a crossbreed hybrids of Pelung and Cemani chickenas a potential meat type breed. Firstly, the breeding between Pelung (one male) and Cemani (4 Females)was performed in semi intensive cage. Secondly, experiment were undertaken using 4 groups of DOC (eachcontain 5 males DOC) : 1) DOC of broiler Cobb 500 vs broiler Cobb 500 ; 2) DOC of Pelung vs Pelung ; 3)DOC of Pelung vs Cemani ; and 4) DOC of Cemani vs Pelung. The animals were kept for 7 weeks (49 days)every week the animals body weight and fur color were recorded. The result showed that there weredifferences in the phenotype characters of between the different crossbreeds. The fur of 5 F1 (femaleCemani vs male Cemani) was black with average body weight 532 ± 39.294 g at week 7. Whilst the fur colorof majority (4/5) F1 (female Pelung vs male Cemani) was black with brown dots with average body weight570 ± 14.445 g at week 7. When Pelung was cross within the same species their F1 fur color was blendedbetween black, brown and whitish with average body weight 652 ± 33.846 g at week 7. It is concluded that,the parent stock in this case the Cemani play a major role in fur color character whereas there ware nodifferences in the body weight at the F1 between the two different parent stock (ie. Pelung, Cemani).
Co-Authors ., Sudarsono Abiyyayumna Rif’at Chasnaurosyiqoh Abrory Agus Cahya Pramana Achmad Amzeri AGUS HERY SUSANTO Agus Nuryanto Agus Setiawan Ainun Nikmati Laily Ajeng Kusumaningtyas Pramono Ajeng Kusumaningtyas Pramono, Ajeng Kusumaningtyas Aji, Katon Waskito Al-Mughni, Eka Wasi? Alin Liana Anahtadiya Nurfa Shochicha Anak Agung Gede Agung Andra Jausa Salsabila Angellya, Bunga Finovel Anisa Parazulfa Anjar Tri Wibowo Annas, Muhammad Sabri Aprilia Sufi Subiastuti Arfa, Namira Nur Ari Indrianto Arief Budiman Aries Bagus Sasongko, Aries Bagus Arimarsetiowati, Rina Asep Rizal Ibrohim Asep Rizal Ibrohim, Asep Asih, Ni Putu Sri Aurantika, Rindu Avia Purnama Saputri Awik Puji Dyah Nurhayati Ayyu Rahayu Aziz Purwantoro Budy Wiryawan Catur Ahda Darojatun Darmastuti Deris Trian Rahmandhias Desi Oktaviani DIAH RACHMAWATI Diah Rachmawati Diah Rachmawati DIAN ARUNI KUMALAWATI Didi Usmadi Didik Indradewa Dinar Nugroho Pratomo Dwi Umi Siswanti Dwijayanti, Vindi E. Suharyanto Edi Suharyadi Eka Noviana Eka Tarwaca Susila Putra Eka Wasi’ Al-Mughni Eko Agus Suyono Elysia Mutiara Azizah Endah Retnaningrum Endang Semiarti Erfianti, Tia Erwin Prastowo Estiyani Indraningsih Esty Nidianti Fadilah Husnun Feren Putri Sholiha Fitranandan, Charisma Asri Fitri Nadifah Galuh Tresnani Ganies Riza Ariestya Ganies Riza Aristya Gebby Agnessya Esa Oktavia Hajrah Hajrah, Hajrah Hani Christin Yambise Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hendry T.S.S.G. Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti Saragih Hermawan, Vera Hetty Nopianasanti Hidayat, Madyan Akmal Hidzroh , Faridatul Himawan Tri Bayu Murti Petrus Hindarsah, Ida Huda, Muhammad Syafi’atol I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Dharma I Made Yudana I Putu Agus Hendra Wibawa I Wayan Suardana I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika IKA MUSTIKA Ikhsan Nur Huda Ikhsan Nur Huda Indra Lesmana Irma Nofitahesti Ishak, Muhammad Alif Ismail, Ismail Isna Mustafiatul Ummah Issirep Sumardi Iwan Gunawan Iwan Roosdianto Iwan Roosdianto Iwan Satibi Jalil, Muhamad Joko Prastowo Joseph Chohansandhika Karmilah, Karmilah Keiko T. Natsuaki Keiko T. Natsuaki, Keiko T. KENJI WAKUI Kristamtini Kristamtini, Kristamtini Kurniawan, Febri Yuda Kusnanda, Prima Sekti Kyswantoro, Yunita Firdha Latifah, Vida Rahma Lily Arsanti Lestari Linda Oktavianingsih M.Pd. ., PROF. DR. I MADE YUDANA, M.Pd. M.Pd. ., PROF.DR.A.A. GEDE AGUNG, M.Pd. Mahfut Makmur, Kurnia Maria Paristiowati Maryani Maryani Maryani Maryani Masita, Masita Masithoh, Dewi Masriany, Masriany Meitha, Karlia Meriem, Selis Miftahudin . Mochammad Imron Awalludin Muazam, Arif Mudasir Mudasir Muhammad Bima Atmaja Muhammad Dylan Lawrie Mumpuni, Nurpuji Nainggolan, Ananto Puradi Narzassi, Angga Bintang Nia Fararid Askar Niken Satuti Nur Handayani Nizma, Nata Dwi Annisa Nugroho Nofriarno Nugroho, Giri Nurachmad Bagas Indriarto Nuraliyah, Tasya Nurcahyati, Vivi Indah Nurdilfa, Nurdilfa Nurhasanah, Anggun Diyan Ovami, Debbi Chyntia Poerwanto, Seonarwan Hery Polikarpia Wilhelmina Bani Prabawani, Ratri Lila Prastiyanto, Muhammad Evy Pratama, Bernardinus Pratiwi, Arini Dian Priyono, Dwi Sendi Puji Wulandari Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Puti Hana Ramadhani Putri Renata, Nellis Nadinda Putro, Karso Suryo Putu Ayu Damayanti Rahmadani, Wenny Deisshinta Rahmadilla Salsabila Mahdison Rajif Iryadi Rajif Iryadi Ratri Lila Prabawani REFLINUR REFLINUR Restiyanti Restiyanti Retnaningrum, Endah Ridesti Rindyastuti Rina Sri Kasiamdari Rini Etika Ranis Rizko Hadi Rizko Hadi, Rizko Roberdi, Roberdi Rohman, Randi Abdur Rozikin, Rozikin Rugayah Rugayah Sahni Damerianta Salsabila, Tantri Ajeng Salma Sartika, Dian Satria Wahyuni SEDYO HARTONO Setiyobudi, Rizal Hermawan Setyorini Widyayanti, Setyorini Shita Tiara Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto, Sigit Siti Subandiyah Slamet Widiyanto Soenghoe Jang Sonjaya, Rasman Sri Darmawati Sri Wahyuningsih Subiastuti, Aprilia Sufi Sudrajat, Rochmat Tri Sukarti Moeljopawiro Sukirno Sukirno Sulaiman, ‪Teuku Nanda Saifullah Sulaiman, ‪Teuku Nanda Saifulllah Sulaiman, Teuku Nanda Saifullah Sulaiman‬, ‪Teuku Nanda Saifullah Sumarmi Sumarmi Sunusi, Fitriani Auriga Supadmi, Francisca Romana Sri Supriyadi Supriyadi Supriyadi Supriyadi Susamto Somowiyarjo Susilawati Susilawati Sutomo Sutomo Sutomo Sutomo Syamsul Arifin Tara Puri Ducha Rahmani TATIK CHIKMAWATI Taufik Hidayatullah Tety Hartatik Teuku Nanda Saifullah Tjoa, Stanley Evander Emeltan Tony Liwang Tri Rini Nuringtyas Tsani, Salma Mutiara Tuty Arisuryanti, Tuty Umar Hafidz Asy’ari Hasbullah, Umar Hafidz Asy’ari Utin Elsya Puspita Utin Elsya Puspita Utomo, Condro Wahyuni, Satria Wayan Tunas Artama Wayan Tunas Artama Wenny Deisshinta Rahmadani, Wenny Deisshinta Wibowo, Wiko Arif Widya Asmara Widyasari, Adristi Shafa Wiko Arif Wibowo Wiko Arif Wibowo Wisnu Nurcahyo Wiwit Probowati Yasir Sidiq Yekti Asih Purwestri Yohana Theresia Maria Astuti, Yohana Theresia Maria Yuanita Rachmawati Yudhistira Nugraha Yuli Astuti Yuliara, I Made Yusuf, Adib Fakhruddin Zolekhah, Dewi